Boa oil composition with anti-inflammatory, allergy-relieving and itching-relieving effects and preparation method thereof

Abstract

The invention belongs to the technical field of preparation of python oil compositions, and particularly relates to a python oil composition with anti-inflammatory, anti-allergy and itching-relieving effects and a preparation method of the python oil composition. The python oil, the humifuse euphorbia herb, the felwort, the beet root and the radix ophiopogonis are specifically combined, so that inflammation can be reduced, nutrition required by the body can be provided, and red, swollen and pruritus can be relieved. Therefore, the composition has anti-inflammatory, allergy-relieving and itching-relieving effects, and can be used for preparing anti-inflammatory, allergy-relieving and itching-relieving products.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of python oil compositions, and in particular relates to a python oil composition with anti-inflammatory, allergy-relieving and itching effects and a preparation method thereof. Background Art

[0002] The skin is the largest organ in the human body, covering the surface of the body and having many important functions. It is not only the first line of defense of the human body, but also participates in many physiological processes such as regulating body temperature, sensing external stimuli, secretion and excretion. However, as time goes by, the self-regulation function of the skin declines, and when it is stimulated by external stimuli, it is easy to cause skin inflammation and allergies, and then symptoms such as itching appear.

[0003] Python oil, also known as python fat or python cream, is a natural oil extracted from python fat. It has a long history of medicinal use. The earliest record of its use as medicine is in "Wu Er Bing Fang". It has many effects. For example, Chinese patent CN 116492250B discloses a scheme for using python oil to treat skin sunburn; Chinese patent CN 116617327A discloses the efficacy of using python oil to treat hemorrhoids. However, there are no reports on python oil compositions with anti-inflammatory, soothing and antipruritic effects. Summary of the invention

[0004] The purpose of the present invention is to provide a python oil composition with anti-inflammatory, soothing and antipruritic effects and a preparation method thereof, which has good anti-inflammatory, soothing and antipruritic effects and is green and safe.

[0005] The invention provides a python oil composition with anti-inflammatory, soothing and antipruritic effects. The python oil composition comprises the following raw materials: python oil, herba euphorbiae, gentiana, beetroot and ophiopogon japonicus.

[0006] Preferably, the python oil composition comprises the following raw materials in parts by mass: 15-20 parts of python oil, 10-12 parts of Euphorbia obesa, 10-12 parts of Gentiana scabra, 6-8 parts of beetroot and 0.5-1 part of Ophiopogon japonicus.

[0007] The present invention provides a method for preparing the python oil composition described in the above technical solution, comprising the following steps:

[0008] Washing and cutting the snake fat in turn to obtain cut snake fat;

[0009] The shredded snake fat is smelted to obtain crude oil;

[0010] mixing the crude oil with an antioxidant to obtain a crude oil composite;

[0011] The crude oil complex, Herba Euphorbiae Ichthyophthirius and Herba Gentianae are mixed and subjected to supercritical carbon dioxide extraction to obtain a mixed extract 1;

[0012] The beetroot and ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0013] The crushed product is mixed with water and then subjected to ultrasonic extraction, and the filtrate is filtered to obtain the mixed extract 2;

[0014] The mixed extract 1 and the mixed extract 2 are mixed to obtain a python oil composition;

[0015] The antioxidant is prepared by mixing ectoine and lecithin in a mass ratio of 1:1.2-1.3.

[0016] Preferably, the refining is ended when the amount of crude oil no longer increases;

[0017] The quenching temperature is 90-100°C.

[0018] Preferably, the mass ratio of the crude oil to the antioxidant is 1:0.02-0.03.

[0019] Preferably, the supercritical carbon dioxide extraction includes: a pressure of 40-45 MPa, a temperature of 30-33° C., and a time of 1.5-2 h.

[0020] Preferably, during the ultrasonic extraction, the mass volume ratio of the crushed material to water is 1 g: 8-10 mL.

[0021] Preferably, the ultrasonic extraction includes: time of 2-3h, temperature of 35-45°C, and power of 410-450w.

[0022] The present invention provides an application of the python oil composition described in the above technical solution, and the application includes: preparing one or more of an anti-inflammatory product, a soothing product and an antipruritic product.

[0023] The present invention provides a medicine with anti-inflammatory, allergy-relieving and itching-relieving effects, and the medicine comprises: the python oil composition described in the above technical solution.

[0024] Beneficial effects:

[0025] The present invention provides a python oil composition with anti-inflammatory, soothing and antipruritic effects, and the python oil composition comprises the following raw materials: python oil, Euphorbia humifusa Willd., Gentiana cabra Bunge, beetroot (Beta vulgaris L.) and Ophiopogon japonicus (Linn.f.) Ker-Gawl. The python oil of the present invention has good moisturizing and repairing effects, can promote skin cell regeneration, and reduce skin dryness and chapped skin; beetroot contains rich vitamins and minerals, can provide nutrients required by the skin, and thus both are beneficial to promote skin repair; Euphorbia humifusa Willd., Gentiana cabra Bunge and Ophiopogon japonicus all have antibacterial and anti-inflammatory effects, that is, the three can synergistically reduce skin inflammation, relieve redness, swelling and itching. Therefore, after the python oil, Euphorbia humifusa Willd., Gentiana cabra Bunge, beetroot and Ophiopogon japonicus are specifically mixed, the composition has anti-inflammatory, soothing and antipruritic effects.

[0026] Based on the above technical advantages, the present invention provides a method for preparing the python oil composition described in the above technical solution, which is conducive to fully releasing the anti-inflammatory, soothing and antipruritic effects of python oil, euphorbia pulex, gentian and beetroot by refining, supercritical carbon dioxide extraction or ultrasonic extraction of different raw materials. Therefore, the above composition can be used to prepare anti-inflammatory, soothing and antipruritic products. DETAILED DESCRIPTION

[0027] Unless otherwise specified, the materials, equipment and methods used are conventional ones.

[0028] In order to further illustrate the present invention, the scheme provided by the present invention is described in detail below in conjunction with embodiments, but they should not be understood as limiting the protection scope of the present invention.

[0029] Example 1

[0030] A method for preparing a python oil composition, comprising the following steps:

[0031] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0032] The specific process is:

[0033] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0034] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0035] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.2) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0036] The cleaned and reserved Herba Euphorbiae Herba and Herba Gentianae were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract 1;

[0037] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0038] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL, and then ultrasonically extracted at 450 W and 45 ° C for 2 h. After filtering, the obtained filtrate was the mixed extract 2;

[0039] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0040] Example 2

[0041] A method for preparing a python oil composition, comprising the following steps:

[0042] The python oil composition is composed of the following raw materials in parts by mass: 20 parts of python oil, 10 parts of Euphorbia obesa, 12 parts of Gentiana scabra, 6 parts of beetroot and 0.5 parts of Ophiopogon japonicus.

[0043] The specific process is:

[0044] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0045] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0046] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.2) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0047] The cleaned and reserved Herba Euphorbiae Herba and Herba Gentianae were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract;

[0048] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0049] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL, and then ultrasonically extracted at 450 W and 45 ° C for 2 h. After filtering, the obtained filtrate was the mixed extract 2;

[0050] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0051] Example 3

[0052] A method for preparing a python oil composition, comprising the following steps:

[0053] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0054] The specific process is:

[0055] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0056] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0057] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.25) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0058] The cleaned and reserved Herba Euphorbiae Herba and Herba Gentianae were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 45 MPa and 33° C. for 1.5 h to obtain a mixed extract;

[0059] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0060] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL, and then ultrasonically extracted at 450 W and 37 ° C for 2 h. After filtration, the obtained filtrate was the mixed extract 2;

[0061] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0062] Comparative Example 1

[0063] A method for preparing a python oil composition, comprising the following steps:

[0064] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia thymifolia L., 10 parts of gentian, 8 parts of beetroot and 1 part of ophiopogon.

[0065] The specific process is:

[0066] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0067] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0068] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.2) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0069] The cleaned and reserved gentian grass and gentian grass were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract 1;

[0070] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0071] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL, and then ultrasonically extracted at 450 W and 45 ° C for 2 h. After filtering, the obtained filtrate was the mixed extract 2;

[0072] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0073] Comparative Example 2

[0074] A method for preparing a python oil composition, comprising the following steps:

[0075] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0076] The specific process is:

[0077] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0078] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0079] The crude oil and the antioxidant (the antioxidant is lecithin) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0080] The cleaned and reserved Herba Euphorbiae Herba and Herba Gentianae were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract;

[0081] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0082] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL, and then ultrasonically extracted at 450 W and 45 ° C for 2 h. After filtering, the obtained filtrate was the mixed extract 2;

[0083] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0084] Comparative Example 3

[0085] A method for preparing a python oil composition, comprising the following steps:

[0086] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0087] The specific process is:

[0088] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0089] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0090] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.2) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0091] The cleaned and reserved Herba Euphorbiae Herba and Herba Gentianae were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract;

[0092] The cleaned beetroot and the cleaned ophiopogon japonicus are mixed and then crushed to obtain a crushed product;

[0093] The crushed material was mixed evenly with water at a mass volume ratio of 1 g:10 mL and then boiled for 2 h. After filtering, the obtained filtrate was the mixed extract 2;

[0094] The mixed extract 1 and the mixed extract 2 are uniformly mixed to obtain a python oil composition.

[0095] Comparative Example 4

[0096] A method for preparing a python oil composition, comprising the following steps:

[0097] The python oil composition is composed of the following raw materials in parts by mass: 15 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0098] The specific process is:

[0099] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0100] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0101] The crude oil and the antioxidant (the antioxidant is prepared by mixing tetrahydromethylpyrimidine carboxylic acid and lecithin in a mass ratio of 1:1.2) are uniformly mixed in a mass ratio of 1:0.02 to obtain a crude oil complex;

[0102] The cleaned and reserved Herba Euphorbiae Herba, Gentianae, and Beetroot were mixed with the crude oil complex, and then extracted with supercritical carbon dioxide at 40 MPa and 33° C. for 1.5 h to obtain a mixed extract;

[0103] The mixed extract and Ophiopogon japonicus are evenly mixed to obtain a python oil composition.

[0104] Comparative Example 5

[0105] A method for preparing a python oil composition, comprising the following steps:

[0106] The python oil composition is composed of the following raw materials in parts by mass: 30 parts of python oil, 10 parts of Euphorbia obesa, 10 parts of Gentiana scabra, 8 parts of beetroot and 1 part of Ophiopogon japonicus.

[0107] The specific process is:

[0108] Clean the python fat and cut it into pieces (there is no requirement for the size of the pieces, as long as it is convenient for subsequent simmering), to obtain the cut snake fat;

[0109] The chopped snake fat is boiled at 90°C. When the amount of crude oil boiled no longer increases, the boiling is stopped to obtain crude oil.

[0110] Mix crude oil with an antioxidant (the antioxidant is a mixture of ectoine and lecithin in a mass ratio of 1:1.2) in a mass ratio of 1:0.02 and mix well to obtain a crude oil complex;

[0111] Mix the cleaned and reserved Euphorbia humifusa and Gentiana scabra with the crude oil complex, and then perform supercritical carbon dioxide extraction at 40 MPa and 33 °C for 1.5 h to obtain a mixed extract 1;

[0112] Mix the cleaned beetroot and the cleaned Ophiopogon japonicus, and then pulverize them to obtain a pulverized product;

[0113] Mix the pulverized product with water in a mass-to-volume ratio of 1 g:10 mL and mix well, then perform ultrasonic extraction at 450 w and 45 °C for 2 h. After filtration, the obtained filtrate is the mixed extract 2;

[0114] Mix the mixed extract 1 and the mixed extract 2 evenly to obtain a python oil composition.

[0115] Application Example 1

[0116] Select healthy mice. After raising them in the same environment for one day, establish a mouse itching model. Specifically: depilate the back of the mice to expose the skin, and then use sandpaper to rub the skin part to ensure that the rubbed part of the mice is only red but not bleeding, so as to obtain a wound of 2 cm × 2 cm; then every 5 minutes, drop 0.05 mL of 0.05% histamine phosphate solution on the wound on the back of the mice until the mice show reactions such as scratching the head with the front paws, scratching the trunk with the hind paws, and biting various parts of the body, indicating that the modeling is successful;

[0117] Take 45 mice with successful modeling, randomly divide them into 9 groups evenly, with 5 mice in each group; take the python oil compositions prepared in Examples 1-3 and Comparative Examples 1-5 as test samples; apply the above test samples to the wounds of the mice at a dose of 0.5 g / kg, and use the treatment of applying an equal amount of clear water as a blank control. Administer the drug 2 times a day for 5 consecutive days; 2 days after stopping the drug, count the reaction conditions of each group of mice, and the results are shown in Table 1; among them,

[0118] Cured means: the wound has no redness and swelling and no adverse reactions of itching;

[0119] Effective means: the wound has slight redness and swelling or has slight itching reactions;

[0120] Ineffective means: the wound is red and swollen and has severe itching reactions.

[0121] Table 1 Cure rates of mice in different treatments

[0122]

[0123]

[0124] Combined with the data in Table 1, it can be seen that after using the python oil compositions prepared in Examples 1-3, it can effectively relieve and cure the itching symptoms and wound inflammation of mice, which is beneficial to the recovery of mouse wounds; while in the comparative examples, after changing the preparation raw materials or preparation methods of the python oil compositions, the prepared python oil compositions are not conducive to relieving the inflammation of mouse wounds or are not conducive to the exertion of their anti-itching effects. Therefore, the highest cure rate of mice in the comparative examples is only 60%.

[0125] Application Example 2

[0126] According to the results in Application Example 1, the python oil compositions prepared in Examples 1-3 and Comparative Example 5 were selected for the following allergy experiment. The specific process is as follows:

[0127] Eighteen healthy guinea pigs were selected and randomly divided into 6 groups with 3 in each group. After being raised in the same environment for one day, the backs of the guinea pigs were depilated, and the depilated area was 3 cm × 3 cm. Then, 1% DNCB was applied to the depilated area. After 5 minutes, the python oil compositions prepared in Examples 1-3 and Comparative Example 5 were applied to the depilated area at a dose of 1 g / kg; at the same time, the treatment with the application of the same dose of dexamethasone was used as the positive control group, and the treatment without applying any substance was used as the blank control.

[0128] The status of the depilated area was scored at 24 h, 48 h, and 72 h after applying the drug. Among them, the scoring criteria are shown in Table 2, and the statistical results are shown in Table 3.

[0129] Table 2 Scoring Criteria

[0130] Points Skin Standards 0 No erythema 1 Mild erythema, barely visible 2 Severe erythema, clearly visible 3 Purple-red spots, eschar formation

[0131] Table 3 Scoring Conditions in Different Treatments

[0132]

[0133]

[0134] Combined with the data in Table 3, it can be seen that after applying the python oil compositions prepared in Examples 1-3 to the backs of guinea pigs, it can better improve the delayed allergic reaction of guinea pigs; its effect is equivalent to that of the hormonal drug dexamethasone in the positive control. It can be seen that the prepared python oil composition of the present invention has the effect of relieving sensitivity; while the python oil composition prepared in Comparative Example 5 has an effect equivalent to that of the blank control.

[0135] In summary, after the specific combination of python oil, Euphorbia humifusa, Gentiana scabra, beet root and Ophiopogon japonicus, the present invention can reduce skin inflammation, relieve swelling, redness and itching through antibacterial and anti-inflammatory effects, and has the effects of anti-inflammation, desensitization and itching relief.

[0136] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A python oil composition having anti-inflammatory, soothing and antipruritic effects, characterized in that: The python oil composition comprises the following raw materials: python oil, herba euphorbiae, herba gentianae, beetroot and ophiopogon japonicus.

2. The python oil composition according to claim 1, characterized in that The python oil composition comprises the following raw materials in parts by mass: 15-20 parts of python oil, 10-12 parts of Euphorbia obesa, 10-12 parts of Gentiana scabra, 6-8 parts of beetroot and 0.5-1 part of Ophiopogon japonicus.

3. The method for preparing the python oil composition according to claim 1 or 2, characterized in that: The steps include: Washing and cutting the snake fat in turn to obtain cut snake fat; The shredded snake fat is refined to obtain crude oil; mixing the crude oil with an antioxidant to obtain a crude oil composite; The crude oil complex, Herba Euphorbiae Ichthyophthirius and Herba Gentianae are mixed and subjected to supercritical carbon dioxide extraction to obtain a mixed extract 1; The beetroot and ophiopogon japonicus are mixed and then crushed to obtain a crushed product; The crushed product is mixed with water and then subjected to ultrasonic extraction, and the filtrate is filtered to obtain the mixed extract 2; The mixed extract 1 and the mixed extract 2 are mixed to obtain a python oil composition; The antioxidant is prepared by mixing ectoine and lecithin in a mass ratio of 1:1.2-1.

3.

4. The preparation method according to claim 3, characterized in that: The refining is terminated when the amount of crude oil no longer increases; The quenching temperature is 90-100°C.

5. The preparation method according to claim 3, characterized in that: The mass ratio of the crude oil to the antioxidant is 1:0.02-0.

03.

6. The preparation method according to claim 3, characterized in that: The supercritical carbon dioxide extraction includes: a pressure of 40-45 MPa, a temperature of 30-33° C., and a time of 1.5-2 h.

7. The preparation method according to claim 3, characterized in that: When performing the ultrasonic extraction, the mass volume ratio of the crushed material to water is 1g:8-10mL.

8. The preparation method according to claim 3 or 7, characterized in that: The ultrasonic extraction includes: time of 2-3h, temperature of 35-45°C, and power of 410-450w.

9. The application of the python oil composition according to claim 1 or 2, comprising: One or more of an anti-inflammatory product, a soothing product and an antipruritic product are prepared.

10. A drug with anti-inflammatory, soothing and antipruritic effects, characterized in that: The medicine comprises: the python oil composition according to claim 1 or 2.

Images