Microfluidic device and microfluidic equipment

Abstract

The invention relates to the technical field of medical detection, in particular to a microfluidic device which comprises a base, a control valve and a driving part. The base is provided with a processing cavity and a plurality of liquid storage cavities, each liquid storage cavity is communicated with a first air channel and a first liquid channel, and each first liquid channel is communicated with the processing cavity; the control valve is movably installed on the base and used for enabling one first air channel to be communicated with the outside or closing all the first air channels. The driving part is arranged in the processing cavity and used for driving the liquid in the processing cavity to flow or driving the liquid in the liquid storage cavity communicated with the first air hole to flow. The invention also relates to a microfluidic device. According to the technical scheme, the detection efficiency can be improved.

Description

Technical Field

[0001] This application relates to the field of medical detection technologies, and particularly to a microfluidic device and a microfluidic equipment. Background Art

[0002] Nucleic acid detection has been widely applied in fields such as biomedicine. It plays a crucial role in many fields such as clinical medicine, food safety, and genetic testing. However, in the process of nucleic acid extraction, in the existing technology, manual operation is mostly used to transfer liquids manually. Not only is the operation complex, but also the effective components in the samples are difficult to be transferred efficiently and sufficiently, resulting in low detection efficiency. Summary of the Invention

[0003] Based on this, an embodiment of this application provides a microfluidic device.

[0004] To solve the above technical problems, an embodiment of this application provides a microfluidic device, which adopts the following technical solutions:

[0005] A microfluidic device includes:

[0006] A base provided with a processing chamber and a plurality of liquid storage chambers. Each of the liquid storage chambers is respectively communicated with a first air duct and a first liquid duct, and each of the first liquid ducts is communicated with the processing chamber;

[0007] A control valve rotatably installed on the base for communicating one of the first air ducts with the outside or closing each of the first air ducts;

[0008] A driving member disposed in the processing chamber for driving the liquid flow in the processing chamber or the liquid flow in the liquid storage chamber communicated with the outside.

[0009] Further, the base is provided with a plurality of first air holes, and each of the first air holes is respectively and correspondingly communicated with each of the first air ducts;

[0010] The control valve includes a valve core movably installed on the base. The valve core is provided with a second air hole for communicating one of the first air holes with the outside;

[0011] Wherein, the valve core is a rotary valve core or a sliding valve core; the rotary valve core is rotatably installed on the base; the sliding valve core is slidably installed on the base.

[0012] Further, the base is further provided with an installation groove, and the valve core is rotatably installed in the installation groove; each of the first air holes is located in the installation groove and communicated with the installation groove.

[0013] Further, the control valve further includes a first hydrophobic breathable membrane, and the first hydrophobic breathable membrane is provided between at least one of the first air holes and the valve core.

[0014] Further, the control valve further includes a gasket provided between the valve core and the base;

[0015] The gasket is provided with third air holes corresponding to and communicating with each of the first air holes one by one;

[0016] Wherein, the third air holes are distributed around the rotation axis of the valve core, and the second air hole is used to communicate one of the third air holes with the outside.

[0017] Further, a flow blocking structure for restricting the liquid flow rate in the first liquid channel is provided in the first liquid channel.

[0018] Further, the driving member includes a push rod and a piston portion; the piston portion is slidably installed in the processing cavity; the push rod is fixedly connected to the piston portion and is used to drive the piston portion to slide back and forth in the processing cavity.

[0019] Further, the base is further provided with a detection cavity, and the detection cavity is communicated with a second air channel communicating with the outside and a second liquid channel communicating with the processing cavity;

[0020] A flow channel valve is provided in the second liquid channel, and the flow channel valve is used to open or close the second liquid channel.

[0021] Further, the flow channel valve is a phase change valve, and a phase change material is provided in the phase change valve; and / or,

[0022] The number of the detection cavities is multiple, and the detection cavities are alternately distributed on the base in sequence; and / or,

[0023] The base is further provided with an exhaust hole, and two ends of the exhaust hole are respectively communicated with the second liquid channel and the outside; the microfluidic device further includes a second hydrophobic breathable membrane, and the second hydrophobic breathable membrane is provided on the base and covers the exhaust hole.

[0024] Further, the second liquid channel includes a liquid inlet channel and a bending channel;

[0025] The processing cavity, the liquid inlet channel, the bending channel and the detection cavity are communicated in sequence;

[0026] At least one bending portion of the bending channel is formed with a receiving groove, and the receiving groove is used to receive the phase change material after the phase change reaction.

[0027] To solve the above technical problems, an embodiment of the present application provides a microfluidic device, which adopts the following technical solutions:

[0028] A microfluidic device, comprising:

[0029] The microfluidic device as described above;

[0030] An auxiliary module, provided in the microfluidic device; the auxiliary device is at least one of a heating module, an ultrasonic module, a magnetic suction module, and an optical detection module.

[0031] Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects: By rotating the control valve to selectively connect the first air passage to the outside, the liquid can be transferred between the liquid storage cavity connected to the first air passage and the processing cavity by the driving member, thereby improving the liquid transfer efficiency and further improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the solution of the present application, the drawings required for the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0033] Figure 1 It is a three-dimensional structural schematic diagram of an embodiment of the microfluidic device of the present application;

[0034] Figure 2 It is a three-dimensional structural exploded schematic diagram of an embodiment of the microfluidic device of the present application;

[0035] Figure 3 It is a structural schematic diagram of the base in an embodiment of the microfluidic device of the present application;

[0036] Figure 4 It is a structural schematic diagram of the base from another perspective in an embodiment of the microfluidic device of the present application;

[0037] Figure 5 It is a structural schematic diagram of the valve core in another embodiment of the microfluidic device of the present application;

[0038] Reference numerals:

[0039] 100. Base; 110. Processing chamber; 111. Lysis solution chamber; 112. Magnetic bead solution chamber; 113. Binding solution chamber; 114. First washing solution chamber; 115. Second washing solution chamber; 116. Elution solution chamber; 120. Liquid storage chamber; 121. First air duct; 122. First liquid duct; 123. Flow blocking structure; 130. First air hole; 140. Installation groove; 150. Detection chamber; 151. Second air duct; 152. Second liquid duct; 1521. Liquid inlet channel; 1522. Bending channel; 1523. Accommodation groove; 153. Flow channel valve; 160. Exhaust hole; 170. Cover body; 180. Chip body; 190. Sealing layer; 200. Control valve; 210. Valve core; 211. Second air hole; 220. First hydrophobic breathable membrane; 230. Sealing gasket; 231. Third air hole; 300. Driving member; 310. Push rod; 320. Piston portion; 400. Second hydrophobic breathable membrane. Detailed implementation manners

[0040] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs; the terms used in the description of this application in the specification are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above drawings are intended to cover non-exclusive inclusion. The terms "first", "second", etc. in the specification and claims of this application or the above drawings are used to distinguish different objects and not to describe a specific order.

[0041] Reference to "embodiment" herein means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of this application. The phrase does not necessarily refer to the same embodiment each time it appears in the specification, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0042] Refer to Figures 1 to 4, an embodiment of the present application provides a microfluidic device, including a base 100, a control valve 200, and a driving member 300; the base 100 is provided with a processing chamber 110 and a plurality of liquid storage chambers 120, each of the liquid storage chambers 120 is respectively communicated with a first air passage 121 and a first liquid passage 122, and each of the first liquid passages 122 is communicated with the processing chamber 110; the control valve 200 is movably installed on the base 100 for communicating one of the first air passages 121 with the outside or closing each of the first air passages 121; the driving member 300 is disposed in the processing chamber 110 for driving the liquid flow in the processing chamber 110 or the liquid flow in the liquid storage chamber 120 communicated with the first air hole 130.

[0043] In this embodiment, the liquid storage chamber 120 is used to store the processing liquid, and the processing chamber 110 is used for processing the sample to be tested, such as adding the processing liquid and the sample to be tested for mixing and reacting.

[0044] In practical applications, by operating the control valve 200, one of the first air passages 121 is selected to communicate with the outside. At this time, by operating the driving member 300, the air pressure in the processing chamber 110 can be adjusted to generate negative pressure or positive pressure. When negative pressure is generated, the outside air enters the liquid storage chamber 120 communicated with it through the first air passage 121, thereby driving the processing liquid in the liquid storage chamber 120 to flow to the processing chamber 110 through the first liquid passage 122 communicated with it, thereby realizing the liquid flow in the liquid storage chamber 120; when positive pressure is generated, the gas in the liquid storage chamber 120 is discharged to the outside air through the first air passage 121 communicated with it, so that a pressure difference is formed between the processing chamber 110 and the liquid storage chamber 120, thereby driving the processing liquid in the processing chamber 110 to flow to the liquid storage chamber 120 through the first liquid passage 122 communicated with it, thereby realizing the liquid flow in the processing chamber 110. In this way, by rotating the control valve 200 to select one of the first air passages 121, the liquid storage chamber 120 communicated with the first air passage 121 can be driven by the driving member 300 to transfer the liquid flow between the processing chamber 110, thereby improving the liquid transfer efficiency and further improving the detection efficiency; moreover, the liquids in the processing chamber 110 and each liquid storage chamber 120 in this application are all internally transferred, reducing the interference of external factors and avoiding sample contamination, thereby improving the detection accuracy.

[0045] Exemplarily, the number of liquid storage chambers 120 is 6, namely a lysis solution chamber 111, a magnetic bead solution chamber 112, a binding solution chamber 113, a first washing solution chamber 114, a second washing solution chamber 115, and an elution solution chamber 116. Among them, the lysis solution chamber 111 stores lysis liquid, the magnetic bead solution chamber 112 stores magnetic bead solution, the binding solution chamber 113 stores proteinase K solution, the first washing solution chamber 114 and the second washing solution chamber 115 respectively store washing solution, and the elution solution chamber 116 stores elution solution. The detection steps are specifically as follows:

[0046] (1) Place the sample to be tested in the lysis solution chamber 111 and react with the lysis liquid in the lysis solution chamber 111;

[0047] (2) By operating the control valve 200, make the first air duct 121 communicated with the binding solution chamber 113 communicate with the outside. At this time, apply negative pressure to the binding solution chamber 113 by operating the driving member 300, so that the proteinase K solution in the binding solution chamber 113 enters the processing chamber 110 through the first liquid duct 122 communicated therewith. Then, operate the control valve 200 again to close the first air duct 121 communicated with the binding solution chamber 113, and make the first air duct 121 communicated with the lysis solution chamber 111 communicate with the outside. Apply negative pressure to the lysis solution chamber 111 by operating the driving member 300, so that the liquid in the lysis solution chamber 111 enters the processing chamber 110 through the first liquid duct 122 communicated therewith. Then start the ultrasonic module to apply ultrasonic waves to the processing chamber 110, so that the proteinase K solution is ultrasonically mixed with the lysis solution (with the sample to be tested), and start the heating module to heat the liquid in the processing chamber 110, thereby accelerating the processing of the liquid in the processing chamber 110. After the processing is completed, apply positive pressure to the processing liquid chamber by operating the driving member 300, so that the liquid in the processing chamber 110 is transported into the lysis solution chamber 111 through the first liquid duct 122 communicated with the lysis solution chamber 111;

[0048] (3) By operating the control valve 200, the first air passage 121 communicating with the lysate chamber 111 is closed, and the first air passage 121 communicating with the magnetic bead liquid chamber 112 is communicated with the outside. At this time, a negative pressure is applied to the magnetic bead liquid chamber 112 by operating the driving member 300, so that the magnetic bead liquid in the magnetic bead liquid chamber 112 enters the processing chamber 110 through the first liquid passage 122 communicating therewith. The magnetic suction module is started to apply a magnetic force to the processing chamber 110, so that the magnetic beads in the magnetic bead liquid are adsorbed to the bottom of the processing chamber 110. Then, a positive pressure is applied to the binding liquid chamber 113 by operating the driving member 300, so that the magnetic bead liquid from which the magnetic beads have been separated is transported into the magnetic bead liquid chamber 112 through the first liquid passage 122 of the magnetic bead liquid chamber 112; Then, the control valve 200 is operated again to close the first air passage 121 communicating with the magnetic bead liquid chamber 112 and communicate the first air passage 121 communicating with the lysate chamber 111 with the outside. A negative pressure is applied to the lysate chamber 111 by operating the driving member 300, so that the liquid in the lysate chamber 111 enters the processing chamber 110 through the first liquid passage 122 communicating therewith. Then, the magnetic suction module is closed and the ultrasonic module is turned on. Under the action of ultrasonic waves, the liquid and magnetic beads in the processing chamber 110 are mixed to quickly extract the biological macromolecule extract from the liquid by the magnetic beads;

[0049] (4) By operating the control valve 200, the first air passage 121 communicating with the lysate chamber 111 is closed, and the first air passage 121 communicating with the first washing liquid chamber 114 is communicated with the outside. At this time, a negative pressure is applied to the first washing liquid chamber 114 by operating the driving member 300, so that the washing liquid in the first washing liquid chamber 114 enters the processing chamber 110 through the first liquid passage 122 communicating therewith to perform the first washing on the magnetic beads, and the ultrasonic module is turned on. The ultrasonic waves act on the processing chamber 110 to improve the mixing efficiency to fully wash the magnetic beads adsorbed with the biological macromolecule extract. Then, the magnetic suction module is started to apply a magnetic force to the processing chamber 110, so that the magnetic beads in the magnetic bead liquid are adsorbed to the bottom of the processing chamber 110. Then, a positive pressure is applied to the processing liquid chamber by operating the driving member 300, so that the liquid in the processing chamber 110 is transported into the first washing liquid chamber 114 through the first liquid passage 122 communicating with the first washing liquid chamber 114;

[0050] (5) By operating the control valve 200, the first air passage 121 communicating with the first washing liquid chamber 114 is closed, and the first air passage 121 communicating with the second washing liquid chamber 115 is communicated with the outside. At this time, by operating the driving member 300 to apply a negative pressure to the second washing liquid chamber 115, the washing liquid in the second washing liquid chamber 115 enters the processing chamber 110 through the first liquid passage 122 communicating therewith to perform a second washing on the magnetic beads, and the ultrasonic module is turned on. The ultrasonic acts on the processing chamber 110 to improve the mixing efficiency to further wash the magnetic beads adsorbed with the biological macromolecule extract. Then, the magnetic suction module is started to apply a magnetic force to the processing chamber 110, so that the magnetic beads in the magnetic bead liquid are adsorbed to the bottom of the processing chamber 110. After that, by operating the driving member 300 to apply a positive pressure to the processing liquid chamber, the liquid in the processing chamber 110 is transported to the second washing liquid chamber 115 through the first liquid passage 122 communicating with the second washing liquid chamber 115;

[0051] (6) By operating the control valve 200, the first air passage 121 communicating with the second washing liquid chamber 115 is closed, and the first air passage 121 communicating with the elution liquid chamber 116 is communicated with the outside. At this time, by operating the driving member 300 to apply a negative pressure to the elution liquid chamber 116, the elution liquid in the elution liquid chamber 116 enters the processing chamber 110 through the first liquid passage 122 communicating therewith to elute the biological macromolecule extract adsorbed on the magnetic beads, thereby obtaining a test sample for subsequent formal detection.

[0052] In some embodiments, referring to Figure 1 and Figure 2 , a cover body 170 for opening or closing the lysis chamber is provided on the base 100, so that it is convenient for the operator to open the lysis chamber through the cover body 170 to put the sample to be tested, or close the cover body 170 to process the sample to be tested in the lysis chamber.

[0053] Referring to Figures 2 to 4 , the base 100 is provided with a plurality of first air holes 130, and each of the first air holes 130 is respectively in one-to-one correspondence and communication with each of the first air passages 121; the control valve 200 includes a valve core 210 movably installed on the base 100, and the valve core 210 is provided with a second air hole 211 for communicating one of the first air holes 130 with the outside.

[0054] In this embodiment, by communicating the second air hole 211 on the valve core 210 with one of the first air holes 130 on the base 100, the first air passage 121 communicating with the first air hole 130 is communicated with the outside, and further, the liquid transfer between the liquid storage chamber 120 communicating with the first air passage 121 and the processing chamber 110 is realized.

[0055] In practical applications, by rotating the valve core 210, the second air hole 211 is communicated with one of the first air holes 130 on the base 100, and the valve core 210 blocks the remaining first air holes 130; or, the second air hole 211 is not communicated with any of the first air holes 130 on the base 100, and the valve core 210 blocks all the first air holes 130.

[0056] In some embodiments, referring to Figures 2 to 4 , the valve core 210 is a rotary valve core, and the rotary valve core is rotatably installed on the base 100. At this time, the first air holes 130 are distributed around the rotation axis of the rotary valve core. When the rotary valve core rotates, the second air hole 211 moves around the rotation axis of the rotary valve core, so that the second air hole 211 is communicated with any one of the first air holes 130 on the base 100.

[0057] In other embodiments, referring to Figure 5 , the valve core is a sliding valve core; the sliding valve core is slidably installed on the base 100. At this time, the first air holes 130 are sequentially arranged along the sliding direction of the sliding valve core. When the sliding valve core slides, the second air hole 211 moves along the sliding direction of the sliding valve core, so that the second air hole 211 is communicated with any one of the first air holes 130 on the base 100.

[0058] In some embodiments, referring to Figure 2 and Figure 3 , the base 100 is further provided with an installation groove 140, and the valve core 210 is rotatably installed in the installation groove 140; each of the first air holes 130 is located in the installation groove 140 and communicated with the installation groove 140.

[0059] In this embodiment, by installing the valve core 210 into the installation groove 140, on the one hand, it is used to protect the connection structure between the valve core 210 and the base 100, thereby improving the connection stability between the two; on the other hand, the valve core 210 can match the shape of the installation groove 140, so that after the valve core 210 is installed in the installation groove 140, the sealing performance of the first air holes 130 on the base 100 is better, thereby avoiding the situation where liquid flows simultaneously in multiple processing chambers 110.

[0060] In some embodiments, referring to Figure 1 , the surface of the valve core 210 away from the installation groove 140 is flush with the surface of the base 100. In this way, on the one hand, the flatness of the surface of the base 100 can be ensured, which is more conducive to the use and installation of the base 100; on the other hand, the valve core 210 can be further protected from external wear.

[0061] In some embodiments, referring to Figure 2 and Figure 3, the control valve 200 further includes a first hydrophobic breathable membrane 220, and the first hydrophobic breathable membrane 220 is provided between at least one of the first air holes 130 and the valve core 210. Understandably, while the hydrophobic breathable membrane enables the airflow to pass through between the first air hole 130 and the second air hole 211, on the one hand, it is used to prevent the liquid in the liquid storage cavity 120 from flowing to the outside after passing through the first air passage 121, the first air hole 130, and the second air hole 211 in sequence, and on the other hand, it is used to prevent the liquid or impurities carried in the outside gas from entering the second air hole 211 through the first air hole 130, so as to avoid the contamination of the liquid in the liquid storage cavity 120.

[0062] In some embodiments, referring to Figure 2 and Figure 3 , the switch assembly further includes a gasket 230 provided between the valve core 210 and the base 100. The gasket 230 is provided with a plurality of third air holes 231, and each of the third air holes 231 is respectively in one-to-one correspondence and communication with each of the first air holes 130. The second air hole 211 is used to communicate one of the third air holes 231 with the outside. Understandably, the gasket 230 is used to improve the connection tightness between the valve core 210 and the base 100, so that when one of the first air holes 130 on the base 100 is in communication with the outside, the communication between the other first air holes 130 and the outside is effectively isolated.

[0063] Furthermore, the gasket 230 is a silica gel gasket or a plastic gasket. Both the silica gel gasket and the plastic gasket have the ability of elastic deformation. When the silica gel gasket / plastic gasket is arranged between the valve core 210 and the base 100, the silica gel gasket / plastic gasket can effectively fill the gap between the valve core 210 and the base 100 by using its elastic deformation ability, thereby further improving the sealing effect of the gasket 230 on the connection between the valve core 210 and the base 100.

[0064] In some embodiments, referring to Figure 2 and Figure 4 , a flow resistance structure 123 is provided in the first liquid passage 122, and the flow rate of the liquid in the first liquid passage 122 is restricted by the flow resistance structure 123, so as to meet different infusion requirements.

[0065] Furthermore, the first liquid passage 122 is a curved passage 1522 or a special-shaped liquid passage, so as to form the flow resistance structure 123, increase the flow resistance of the liquid in the first liquid passage 122, elongate the streamline of the first liquid passage 122, and slow down the overall flow rate of the liquid.

[0066] In some embodiments, referring to Figure 2 and Figure 4, the driving member includes a push rod 310 and a piston portion 320; the piston portion 320 is slidably installed in the processing chamber 110; the push rod 310 is fixedly connected to the piston portion 320 and is used to drive the piston portion 320 to slide back and forth in the processing chamber 110.

[0067] In this embodiment, the opposite ends of the piston portion 320 in the sliding direction thereof respectively have a driving end and a mounting end, wherein the driving end is arranged towards the inner end of the processing chamber 110, the mounting end is arranged away from the inner end of the processing chamber 110, and the push rod 310 is installed on the mounting end; moreover, the piston portion 320 is in interference fit with the inner wall of the processing chamber 110. By operating the push rod 310 to drive the piston portion 320 to move back and forth in the processing chamber 110, a negative pressure for making the liquid in the liquid storage chamber 120 flow or a positive pressure for making the liquid in the processing chamber 110 flow is generated.

[0068] In some embodiments, referring to Figure 2 and Figure 4 , the base 100 is further provided with a detection chamber 150, and the detection chamber 150 is communicated with a second air passage 151 and a second liquid passage 152; the second air passage 151 is also communicated with the outside; the second liquid passage 152 is also communicated with the processing chamber 110; a flow path valve 153 is arranged in the second liquid passage 152, and the flow path valve 153 is used to open or close the second liquid passage 152.

[0069] In this embodiment, after the liquid transfer cooperation is carried out between the processing chamber 110 and each liquid storage chamber 120 to obtain a test sample, the control valve 200 is operated to close each first air passage 121, and the second liquid passage 152 is opened through the flow path valve 153. A positive pressure is applied to the processing chamber 110 by operating the driving member 300, so as to make the test sample in the processing chamber 110 flow into the detection chamber 150 through the second liquid passage 152 for detection.

[0070] Exemplarily, freeze-dried balls are stored in the detection chamber 150; after the test sample in the processing chamber 110 flows into the detection chamber 150 through the second liquid passage 152, the freeze-dried balls in the detection chamber 150 react with the test sample, and cooperate with the heating module to carry out a PCR amplification reaction. After the reaction is completed, the optical detection module is turned on to realize the fluorescence detection of the test sample.

[0071] In some embodiments, the shape of the detection chamber 150 is circular or square.

[0072] In some embodiments, the flow channel valve 153 is a phase change valve, and a phase change material is provided in the phase change valve. Understandably, the phase change material can be a solid-liquid phase change material; initially, the solid-liquid phase change material is in a solid state, and the phase change valve is in a state of closing the second liquid channel 152; by heating the phase change material in the phase change valve, when the solid-liquid phase change material reaches the phase change melting point, the solid-liquid phase change material is converted from a solid state to a liquid state, so that the phase change valve switches to a state of opening the second liquid channel 152. At this time, the test sample in the processing chamber 110 can enter the detection chamber 150 through the second liquid channel 152 for detection.

[0073] Further, the solid-liquid phase change material is a paraffin material. The paraffin material is normally in a solid state and melts and is converted into a liquid state after reaching the phase change melting point, thereby realizing the switching of the phase change valve from closing the second liquid channel 152 to opening the second liquid channel 152.

[0074] Further, the shape of the paraffin material is square or spherical.

[0075] In some embodiments, refer to Figure 2 and Figure 4 , the number of the detection chambers 150 is multiple, and the detection chambers 150 are alternately distributed on the base 100 in sequence. In this way, the space on the base 100 is fully utilized, the layout rationality of the detection chambers 150 is improved, and the structure of the base 100 is made more compact.

[0076] In some embodiments, the base 100 is further provided with an exhaust hole 160, and two ends of the exhaust hole 160 are respectively communicated with the second liquid channel 152 and the outside; the microfluidic device further includes a second hydrophobic breathable membrane 400, and the second hydrophobic breathable membrane 400 is arranged on the base 100 and covers the exhaust hole 160. In this way, the second hydrophobic breathable membrane 400 is used to prevent the liquid in the detection chamber 150 from flowing out to the outside after passing through the second air channel 151 and the exhaust hole 160 in sequence, and also prevent the liquid from the outside from entering the detection chamber 150 through the second air channel 151 from the exhaust hole 160.

[0077] In some embodiments, the second liquid channel 152 includes a liquid inlet channel 1521 and a bending channel 1522, and the processing chamber 110, the liquid inlet channel 1521, the bending channel 1522 and the detection chamber 150 are communicated in sequence; at least one bending portion of the bending channel 1522 is formed with a receiving groove 1523, and the receiving groove 1523 is used for receiving the phase change material after the phase change reaction, so as to prevent the phase change material after the phase change reaction from entering the detection chamber 150 or blocking the second liquid channel 152 and affecting the detection.

[0078] Exemplarily, the phase change material is a paraffin material; the paraffin material is initially in a solid state. After undergoing a phase change reaction and converting from a solid state to a liquid state, the liquid paraffin material has relatively poor fluidity compared to a liquid. Therefore, the curved channel 1522 is used to collect the liquid paraffin material to prevent it from entering the detection chamber 150 or blocking the second liquid channel 152, which may affect the detection.

[0079] In some embodiments, a brush structure is provided in the accommodation groove 1523. There are some corners between the brush structures to accommodate the paraffin material after the phase change reaction, and the paraffin material after the phase change reaction will also partially adhere to the brush structure. The brush structure can prevent the paraffin material after the phase change reaction from blocking the second liquid channel 152. The setting of this structure can increase the flow resistance of the paraffin material after the phase change reaction, thereby further preventing it from entering the detection chamber 150.

[0080] In some embodiments, referring to Figure 2 and Figure 4 , the base 100 includes a chip body 180 and a sealing layer 190 that are installed in an opposing manner. The chip body 180 and the sealing film are bonded to form the first liquid channel 122, the second liquid channel 152, the first air channel 121, and the second air channel 151.

[0081] Among them, the chip body 180 and the sealing film can be bonded and sealed by means such as laser welding, ultrasonic welding, and hot pressing welding.

[0082] Furthermore, the sealing layer 190 can be one of double-sided tape, pressure-sensitive adhesive, sealing film, and plastic sheet. Among them, double-sided tape and pressure-sensitive adhesive require strong adhesiveness, water resistance, high temperature resistance (above 95 °C), and good biocompatibility, and will not react with the biological reagents in the liquid storage chamber 120; the sealing film can be a PC material or a PMMA material, and the plastic sheet also needs to be resistant to high temperature of 95 °C, and the material has good biocompatibility and will not react with the reagent during the contact with the reagent.

[0083] The embodiment of the present application further provides a microfluidic device, including the microfluidic device and an auxiliary device as described above. The auxiliary device is at least one of a heating module, an ultrasonic module, a magnetic suction module, and an optical detection module.

[0084] In practical applications, by operating the control valve 200, one of the first air channels 121 is selected to communicate with the outside. At this time, by operating the driving member 300, the air pressure in the processing chamber 110 can be adjusted to generate negative pressure or positive pressure. When negative pressure is generated, the outside atmosphere enters the liquid storage chamber 120 communicating with it through the first air channel 121, thereby driving the processing liquid in the liquid storage chamber 120 to flow through the first liquid channel 122 communicating with it into the processing chamber 110, thus realizing the flow of the liquid in the liquid storage chamber 120. When positive pressure is generated, the gas in the liquid storage chamber 120 is discharged to the outside atmosphere through the first air channel 121 communicating with it, so that a pressure difference is formed between the processing chamber 110 and the liquid storage chamber 120, thereby driving the processing liquid in the processing chamber 110 to flow through the first liquid channel 122 communicating with it into the liquid storage chamber 120, thus realizing the flow of the liquid in the processing chamber 110. In this way, only by rotating the control valve 200 to select one of the first air channels 121, the liquid storage chamber 120 corresponding to the first air channel 121 can be driven by the driving member 300 to transfer the liquid flow between the processing chamber 110, thereby improving the liquid transfer efficiency and further improving the detection efficiency. Moreover, in the present application, the liquids in the processing chamber 110 and each liquid storage chamber 120 are internally transferred, reducing the interference of external factors and avoiding sample contamination, thereby improving the detection accuracy.

[0085] Secondly, after transferring the liquid in the liquid storage chamber 120 to the processing chamber 110, one of the heating module, ultrasonic module, and magnetic suction module can be cooperated to improve the detection efficiency. Moreover, when formally testing the processed test sample, fluorescence detection can be cooperated with the optical detection module.

[0086] Furthermore, the heating module includes at least two constant temperature zones, and the temperature of each constant temperature zone is different and is used to heat the processing chamber 110.

[0087] In this embodiment, with the setting of multiple constant temperature zones with different temperatures, by moving the base 100, the processing chamber 110 can be moved between the constant temperature zones with different temperatures, without waiting for the temperature rise and fall time, shortening the reaction time and improving the detection efficiency.

[0088] Obviously, the embodiments described above are only a part of the embodiments of this application, rather than all embodiments. The accompanying drawings present preferred embodiments of this application, but do not limit the patent scope of this application. This application can be implemented in many different forms. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosed content of this application more thorough and comprehensive. Although this application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing specific embodiments, or perform equivalent replacements for some of the technical features. Any equivalent structures made by using the content of the specification and drawings of this application, directly or indirectly applied in other related technical fields, are equally within the scope of patent protection of this application.

Claims

1. A microfluidic device, characterized in that, Comprising: A base provided with a processing chamber and a plurality of liquid storage chambers. Each of the liquid storage chambers is respectively communicated with a first air duct and a first liquid duct, and each of the first liquid ducts is communicated with the processing chamber; A control valve movably installed on the base for communicating one of the first air ducts with the outside or closing each of the first air ducts; A driving member disposed in the processing chamber for driving the liquid flow in the processing chamber or the liquid flow in the liquid storage chamber communicated with the outside.

2. The microfluidic device according to claim 1, characterized in that, The base is provided with a plurality of first air holes, and each of the first air holes is respectively communicated with each of the first air ducts in one-to-one correspondence; The control valve includes a valve core movably installed on the base. The valve core is provided with a second air hole for communicating one of the first air holes with the outside; Wherein, the valve core is a rotary valve core or a sliding valve core; the rotary valve core is rotatably installed on the base; the sliding valve core is slidably installed on the base.

3. The microfluidic device according to claim 2, characterized in that, The base is further provided with an installation groove, and the valve core is rotatably installed in the installation groove; each of the first air holes is located in the installation groove and communicated with the installation groove.

4. The microfluidic device according to claim 2, characterized in that, The control valve further includes a first hydrophobic breathable membrane, and the first hydrophobic breathable membrane is disposed between at least one of the first air holes and the valve core.

5. The microfluidic device according to claim 2, characterized in that, The control valve further includes a gasket disposed between the valve core and the base; The gasket is provided with a plurality of third air holes, and each of the third air holes is respectively communicated with each of the first air holes in one-to-one correspondence. The second air hole is used for communicating one of the third air holes with the outside.

6. The microfluidic device according to any one of claims 1 to 5, characterized in that, A flow blocking structure for restricting the liquid flow rate in the first liquid duct is provided in the first liquid duct; and / or, The driving member includes a push rod and a piston portion; the piston portion is slidably installed in the processing chamber; the push rod is fixedly connected to the piston portion for driving the piston portion to slide back and forth in the processing chamber.

7. The microfluidic device according to any one of claims 1 to 5, characterized in that, The base is further provided with a detection chamber, the detection chamber is communicated with a second air duct and a second liquid duct, the second air duct is further communicated with the outside, and the second liquid duct is further communicated with the processing chamber; A flow path valve is provided in the second liquid duct, and the flow path valve is used for opening or closing the second liquid duct.

8. The microfluidic device according to claim 7, characterized in that, The flow path valve is a phase change valve, and a phase change material is provided in the phase change valve; and / or, The number of the detection chambers is multiple, and the detection chambers are sequentially and alternately distributed on the base; and / or, The base is further provided with an exhaust hole, and two ends of the exhaust hole are respectively communicated with the second liquid duct and the outside; the microfluidic device further includes a second hydrophobic breathable membrane disposed on the base and covering the exhaust hole.

9. The microfluidic device according to claim 8, characterized in that, The second liquid duct includes a liquid inlet channel and a bending channel; The processing chamber, the liquid inlet channel, the bending channel and the detection chamber are sequentially communicated; At least one bending portion of the bending channel is formed with a receiving groove for receiving the phase change material after the phase change reaction.

10. A microfluidic device, characterized in that, Comprising: The microfluidic device according to any one of claims 1 to 9; An auxiliary module disposed on the microfluidic device; the auxiliary device is at least one of a heating module, an ultrasonic module, a magnetic suction module and an optical detection module.

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