Application of cubebolide and Platycladus orientalis leaf extract containing cubebolide in promoting hair growth and preventing hair loss

Abstract

The present invention provides the use of cubebone and a Platycladus orientalis leaf extract containing cubebone for promoting hair growth and preventing hair loss. This relates to the technical field of daily chemical products. The inventors employed network pharmacology to systematically analyze the material basis and key targets for the hair growth / hair loss prevention effects of Platycladus orientalis leaf, providing a theoretical basis for the hair loss prevention / hair growth promotion effects of Platycladus orientalis leaf and its active ingredient, cubebone. The 5α-reductase inhibition experiment explored the anti-hair loss / hair growth potential of cubebone, an active ingredient in Platycladus orientalis leaf extract, providing a theoretical basis for the application of Platycladus orientalis leaf extract and cubebone.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, in particular to application of cubebone and a Platycladus orientalis leaf extract containing cubebone in promoting hair growth and preventing hair loss. Background Art

[0002] Alopecia is a common skin disease that affects patients' quality of life and has a psychological impact. The cause and mechanism of alopecia are not yet fully elucidated, but it is generally believed that it may be caused by a combination of genetics and environmental stimuli.

[0003] Modern medical research has demonstrated that genetic susceptibility and abnormal androgen metabolism in the scalp are the primary factors in the development of androgenic alopecia. 5α-reductase controls androgen metabolism, converting the androgen testosterone (T) into dihydrotestosterone (DHT). As a potent androgen, DHT has a higher affinity for androgen receptors than testosterone. High levels of DHT in the body contribute to androgenic alopecia. Clinically, 5α-reductase inhibitors, such as finasteride and dunasteride, are used to treat androgenic alopecia by inhibiting 5α-reductase activity, selectively blocking DHT synthesis and reducing androgen levels in the body. However, these drugs are often associated with significant side effects.

[0004] Platycladus orientalis leaf is widely used in traditional Chinese medicine to treat hair loss. It is believed to cool the blood and stop bleeding, clear away heat and detoxify, effectively inhibit scalp inflammation, and promote healthy hair follicles. For hair loss caused by excessive oil secretion or clogged hair follicles, Platycladus orientalis leaf shampoo can help improve the scalp environment, promote hair growth, and thus reduce hair loss. Platycladus orientalis leaf also regulates scalp oil secretion. It can help control excessive oil production, thereby reducing greasiness and dandruff. This is very useful for those who often experience scalp discomfort due to excessive oil secretion or who need to wash their hair frequently. As a natural ingredient, Platycladus orientalis leaf is relatively safe and suitable for most people. However, the active ingredients in traditional Chinese medicine are complex and diverse, making standards difficult to control and their targets difficult to define.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] One of the objects of the present invention is to provide a use of cubeb lactone and a Platycladus orientalis leaf extract containing cubeb lactone in the preparation of a product for promoting hair growth and / or preventing hair loss, so as to at least solve one of the technical problems existing in the above-mentioned prior art.

[0007] A second object of the present invention is to provide a use of cubeb lactone in inhibiting 5α-reductase or preparing a product that inhibits 5α-reductase.

[0008] The third object of the present invention is to provide the use of Platycladus orientalis leaf extract containing cubeb lactone in promoting the growth of dermal papilla cells.

[0009] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are adopted:

[0010] In a first aspect, the present invention provides the use of cubeb lactone and a Platycladus orientalis leaf extract containing cubeb lactone in the preparation of a product that promotes hair growth and / or prevents hair loss.

[0011] In a second aspect, the present invention provides the use of cubeb lactone in inhibiting 5α-reductase or preparing a product for inhibiting 5α-reductase.

[0012] In a third aspect, the present invention provides the use of a Platycladus orientalis leaf extract containing cubeb lactone in promoting the growth of dermal papilla cells.

[0013] Furthermore, the Platycladus orientalis leaf extract is obtained by alcohol extraction.

[0014] Furthermore, the preparation method of the Platycladus orientalis leaf extract includes uniformly mixing Platycladus orientalis leaf crude powder and 95% ethanol in a volume ratio of 1:12-17, soaking, ultrasonicating, and filtering to obtain the Platycladus orientalis leaf extract.

[0015] Furthermore, the crude arborvitae leaf powder and the solvent are evenly mixed in a volume ratio of 1:15.

[0016] Furthermore, the soaking time is at least 1 hour.

[0017] Furthermore, the ultrasonic time is 20 to 50 minutes.

[0018] Furthermore, the soaking, ultrasonication and filtration are performed at least twice, and the filtrates are combined after filtration to obtain the Platycladus orientalis leaf extract.

[0019] Furthermore, the particle size of the Platycladus orientalis leaf coarse powder is 40-60 mesh.

[0020] The present invention provides the use of cubebone and a Platycladus orientalis leaf extract containing cubebone in the preparation of products that promote hair growth and / or prevent hair loss. The inventors used network pharmacology to systematically analyze the material basis and key targets of Platycladus orientalis leaf's hair growth promotion / anti-hair loss effects, providing a theoretical basis for the anti-hair loss / promoting effects of Platycladus orientalis leaf and its active ingredient, cubebone. 5α-reductase inhibition experiments were conducted to explore the anti-hair loss / hair growth potential of cubebone, an active ingredient in Platycladus orientalis leaf extract, providing a theoretical basis for the application of Platycladus orientalis leaf extract and cubebone. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 A Venn diagram of active ingredients of Platycladus orientalis leaf extract and hair loss targets provided in Example 1 of the present invention;

[0023] Figure 2 This is a network diagram of core targets of the Platycladus orientalis leaf extract active in preventing hair loss / promoting hair growth provided in Example 1 of the present invention;

[0024] Figure 3 The inhibition rate of cubeb lactone on 5α-reductase provided in Example 2 of the present invention;

[0025] Figure 4 The results of the effects of the Platycladus orientalis leaf alcohol extract and Platycladus orientalis leaf water extract on the viability of human dermal papilla cells provided in Example 3 of the present invention;

[0026] Figure 5 This is a diagram showing changes in the growth of hair on the back skin of mice promoted by the alcohol extract of Platycladus orientalis leaves provided in Example 3 of the present invention. DETAILED DESCRIPTION

[0027] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meaning and scope of the terms should be clear; however, in the event of any potential ambiguity, the definitions provided herein take precedence over any dictionary or external definitions. In this application, the use of "or" means "and / or" unless otherwise stated. In addition, the use of the term "including" and other forms is non-limiting.

[0028] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] On one hand, the present invention provides the use of cubeb lactone and a Platycladus orientalis leaf extract containing cubeb lactone in the preparation of a product for promoting hair growth and / or preventing hair loss.

[0030] The inventors used network pharmacology to systematically analyze the material basis and key targets of Platycladus orientalis leaf's hair growth-promoting / anti-hair loss effects, providing a theoretical basis for the effectiveness of Platycladus orientalis leaf and its active ingredient, cubebone, in preventing and promoting hair growth. 5α-reductase inhibition experiments were conducted to explore the potential of cubebone, an active ingredient in Platycladus orientalis leaf extract, to prevent and promote hair growth, providing a theoretical basis for the application of Platycladus orientalis leaf extract and cubebone.

[0031] Among them, cubeb lactone is the active ingredient of Platycladus orientalis leaf extract, and its CAS number is 26543-89-5.

[0032] According to another aspect of the present invention, there is also provided the use of cubeb lactone in inhibiting 5α-reductase or preparing a product for inhibiting 5α-reductase.

[0033] According to another aspect of the present invention, there is also provided the use of a Platycladus orientalis leaf extract containing cubebolide in promoting the growth of dermal papilla cells.

[0034] Cubebone is a fat-soluble component that is insoluble in water. The content of fat-soluble components such as cubebone in water extracts is limited. Alcohol-extracted Platycladus orientalis leaf extract is more effective than water-extracted Platycladus orientalis leaf extract in improving dermal papilla cell viability. In some specific embodiments, the Platycladus orientalis leaf extract containing cubebone is prepared by alcohol extraction. In some specific embodiments, the Platycladus orientalis leaf extract is prepared by a method comprising uniformly mixing Platycladus orientalis leaf coarse powder with 95% ethanol in a volume ratio of 1:12 to 17, soaking, sonicating, and filtering to obtain the Platycladus orientalis leaf extract.

[0035] In some specific embodiments, the crude Platycladus orientalis leaf powder and the solvent are evenly mixed in a volume ratio of 1:15.

[0036] In some specific embodiments, the soaking time is at least 1 hour. In some specific embodiments, the ultrasonication time is 20 to 50 minutes. In some specific embodiments, the soaking, ultrasonication, and filtration are performed at least twice, and the filtrates are combined after filtration to obtain the Platycladus orientalis leaf extract. In some specific embodiments, the particle size of the Platycladus orientalis leaf coarse powder is 40 to 60 mesh.

[0037] The present invention is further described below by way of examples. Unless otherwise specified, the materials in the examples were prepared according to existing methods or directly purchased from the market.

[0038] Preparation of Platycladus orientalis leaf alcohol extract containing cubeb lactone: dry Platycladus orientalis leaf coarse powder crushed to 40-60 mesh size was mixed with 95% ethanol at a solid-liquid ratio of 1:15, stirred evenly, soaked at room temperature for 1 hour, placed in an ultrasonic instrument for 30 minutes, filtered and the residue was centrifuged and then repeated the extraction twice. The filtrate was combined and concentrated on a rotary evaporator to obtain the Platycladus orientalis leaf alcohol extract, and stored at -20°C.

[0039] Preparation of Platycladus orientalis leaf water extract: The difference from the preparation of Platycladus orientalis leaf alcohol extract is that deionized water is used instead of 95% ethanol to prepare the Platycladus orientalis leaf water extract.

[0040] Example 1 Network pharmacology analysis of Platycladus orientalis extract

[0041] 1. Network pharmacology approach for Platycladus orientalis leaf extract

[0042] (1) Screening of active ingredients and prediction of active ingredient targets of Platycladus orientalis leaf extract

[0043] Using Platycladus orientalis leaves and its Latin name Platycladus orientalis as search terms, all its chemical components were retrieved by TCMSP. Chemical components that simultaneously met the requirements of molecular weight <500 mol mass and drug-likeness (DL) ≥0.18, as well as active components of Platycladus orientalis leaf extract obtained by liquid chromatography-mass spectrometry, were screened as potential active ingredients. Unpredicted active ingredients were supplemented based on literature reports to construct a Platycladus orientalis leaf active ingredient dataset.

[0044] The 3D structure and SMILES format of the active components of Platycladus orientalis leaves were obtained from the Pubchem database. These structures were then imported into the Swisstargetprediction database to obtain the corresponding predicted targets. Targets with a probability greater than 0 were selected. The Uniport database was used to convert target names to unified gene names, and the species was restricted to Homosapiens.

[0045] (2) Prediction of hair loss disease targets

[0046] We searched for genes associated with hair loss by entering the keyword "alopecia" and its synonym "hair loss" into the GeneCard, OMIM, and TTD databases. We screened for targets with a score greater than 10 in the GeneCard database. We also retrieved all disease targets from the OMIM and TTD databases. The results were then integrated and deduplicated to identify targets for hair loss diseases.

[0047] (3) Construction of PPI network of active ingredients of Platycladus orientalis leaves and screening of core ingredients for hair loss targets

[0048] The target genes of the active ingredients of Platycladus orientalis leaves were intersected with target genes associated with hair loss, and a Venn diagram was constructed to identify common gene targets of Platycladus orientalis leaves for treating hair loss. The identified intersection targets were uploaded to the String database, with Homo sapiens selected as the study species. The "tsv" format file containing the protein interaction relationships was downloaded. The results were then imported into Cytoscape 3.10.0 software for visualization and analysis, followed by topological analysis using the CytoNCA plug-in. The core components of the drug were identified based on the "Degree" values ​​in the software analysis results.

[0049] The intersection targets identified by mapping were uploaded to the String database, "Homo sapiens" was selected as the research species, and the "tsv" format file of the protein interaction relationship was downloaded. The results were then imported into Cytoscape 3.10.0 software for visualization and analysis. The Centiscape plug-in tool was then used for analysis. The key core targets of Platycladus orientalis leaf extract for preventing hair loss and promoting hair growth were screened based on the "Betweenness," "Closeness," and "Degree" values ​​in the software analysis results.

[0050] 2. Analysis of network pharmacology results of Platycladus orientalis leaf extract

[0051] (1) Screening of active ingredients and prediction of active ingredient targets of Platycladus orientalis leaf extract

[0052] A total of 63 active ingredients were screened from Platycladus orientalis leaf extract. The targets of these 63 active ingredients were merged and duplicates were removed, resulting in a total of 630 potential targets for the active ingredients of Platycladus orientalis leaf extract.

[0053] (2) Prediction of hair loss disease targets

[0054] The hair loss-related targets were summarized by combining Genecards, OMIM, and TTD databases. After merging, duplicate values ​​were deleted, and finally 1,648 hair loss-related targets were obtained.

[0055] The active ingredient target set of the screened Platycladus orientalis leaf extract and the hair loss disease target set were used to draw a Venn diagram of Platycladus orientalis leaf extract ingredients and hair loss targets, as shown in Figure 1 As shown, 124 common targets for Platycladus orientalis leaf extract and hair loss were obtained. These overlapping genes are important genes for Platycladus orientalis leaf extract to promote hair growth and prevent hair loss.

[0056] (3) Construction of PPI network and screening of core components of Platycladus orientalis leaf extract active ingredients for hair loss targets

[0057] Using Cytoscape 3.10.0 software, a drug-active ingredient-target-disease interaction network was constructed. Further analysis of the resulting network revealed 124 nodes and 1,726 edges, each representing the relationship between Platycladus orientalis leaf extract and the active ingredient, the active ingredient and the target, and the target and the disease.

[0058] Degree (degree centrality) is closely related to the target of hair loss disease. The larger the Degree value corresponding to the active ingredient, the closer its relationship with hair loss prevention. After sorting the characteristic parameters of the network nodes of the potential active ingredients of Platycladus orientalis leaves by Degree (Degree>20), it was found that the Degree of cubeb lactone was the largest (30). Therefore, cubeb lactone may play a leading role in the treatment of hair loss by Platycladus orientalis leaves.

[0059] The target protein interaction network shows that 26 potential core targets of Platycladus orientalis leaf extract for treating hair loss / promoting hair growth were screened out, namely: STAT3, TP53, ERBB2, EGFR, BCL2, AKT1, CTNNB1, MAPK1, IL2, CCND1, EP300, APP, ALB, MTOR, JUN, TNF, AR, ESR1, SRC, SNCA, ACE, PPARG, CXCL8, ESR2, SHH, CYP19A1. The core target information of Platycladus orientalis leaf extract active ingredients for preventing hair loss / promoting hair growth is shown in Table 1. The core target network diagram of Platycladus orientalis leaf extract active ingredients for preventing hair loss / promoting hair growth is shown in Table 1. Figure 2 shown.

[0060] Table 1

[0061]

[0062] Example 2 Study on the Anti-hair Loss / Hair Growth-Promoting Effect of Cubeb Lactone

[0063] 1. Research Methods for the Anti-Hair Loss / Hair Growth Effects of Cubeb Lactone

[0064] Cubeb lactone: CAS 26543-89-5, purchased from TargetMol.

[0065] (1) Preparation of experimental samples: Dissolve cubeb lactone in anhydrous ethanol to prepare cubeb lactone stock solutions with concentrations of 20 μmol / L, 40 μmol / L, 80 μmol / L, 160 μmol / L, and 320 μmol / L, respectively.

[0066] (2) 5α-reductase inhibition rate test

[0067] ① The test sample stimulates the standard enzyme

[0068] A. Prepare standardized enzyme, dilute to a concentration of 80 U / L, and spread on a cell culture plate with a volume of 100 μL added to each well.

[0069] B. Prepare the sample (the experimental sample in (1)). Based on the pre-experimental results, set the test sample concentration to a certain concentration point to stimulate the standard enzyme. Sterile enzyme-free water or anhydrous ethanol were used as the blank control for stimulation at a concentration of 100 μL. The reaction was continued for 6 hours. The supernatant was then transferred to an EP tube and centrifuged to obtain the supernatant for testing.

[0070] C. Use 5α-reductase kit to detect enzyme amount.

[0071] ② Kit detection

[0072] A. Sample Addition: Accurately add 50 μL of the standard and sample to the ELISA coated plate. Add the sample to the bottom of the plate well, avoiding touching the well wall as much as possible, and gently shake to mix.

[0073] B. Incubation: Seal the plate with sealing film and incubate at 37°C for 30 min.

[0074] C. Liquid preparation: Dilute the 30-fold concentrated washing solution with 30-fold distilled water and set aside.

[0075] D. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing solution, let it stand for 30 seconds, then discard, repeat this 5 times, and pat dry.

[0076] E. Add enzyme: Add 50 μL of enzyme-labeled reagent to each well, except for the blank well.

[0077] F. Incubation: Same operation as B.

[0078] G. Washing: Same operation as D.

[0079] H. Color development: Add 50 μL of color developer A to each well, then add 50 μL of color developer B, gently shake to mix, and develop the color at 37°C in the dark for 15 minutes.

[0080] I. Stop: Add 50 μL of stop solution to each well to terminate the reaction (the blue color will immediately turn yellow).

[0081] J. Measurement: Using a blank as the zero value, measure the absorbance (OD) of each well at 450 nm. Measurement should be performed within 15 minutes after adding the stop solution.

[0082] ③The calculation formula of the enzyme activity of the sample to be tested is as follows:

[0083] .

[0084] 2. Research results on the effects of cubeb lactone on preventing hair loss and promoting hair growth

[0085] The inhibition rate of cubeb lactone on 5α-reductase is shown in Table 2 and Figure 3 As shown, the inhibition rate of cubebone against 5α-reductase increases with increasing concentration, indicating that the inhibitory activity of cubebone against 5α-reductase exhibits a clear dose-response relationship within a certain concentration range. The IC50 of cubebone against 5α-reductase is 127.938 μmol / L. Cubebone can prevent hair loss and promote hair growth by inhibiting 5α-reductase.

[0086] Table 2

[0087]

[0088] Example 3 Evaluation of the Anti-hair Loss / Hair Growth Promoting Effects of Platycladus orientalis Leaf Alcohol Extract

[0089] 1. Evaluation Method

[0090] (1) Effects of Platycladus orientalis leaf alcohol extract and Platycladus orientalis leaf water extract on the proliferation of human dermal papilla cells

[0091] ① Cell seeding plate: human dermal papilla cells in logarithmic growth phase were taken and the cell density was adjusted to 5×10 4 100 μL / well was inoculated into a 96-well plate (the edge wells were filled with sterile PBS) and cultured at 37°C with 5% CO2.

[0092] ② Grouped drug administration: After the cells adhered to the wall, the culture medium containing different concentration gradients of Platycladus orientalis leaf ethanol extract and different concentrations of Platycladus orientalis leaf water extract were replaced for cultivation. The concentration gradient included 5μg / mL, 10μg / mL, 50μg / mL, 100μg / mL and 200μg / mL, and a blank control group (Control) and a positive control group (Minoxidil) were set up respectively. Each treatment had 7 replicates, and minoxidil was used in the positive control group.

[0093] ③CCK8 assay: After 48 hours of drug treatment, aspirate the supernatant and add 110 μL / well of diluted CCK8 working solution. Gently shake the culture plate several times and continue incubation at 37°C, 5% CO2 for 2 hours. Measure the absorbance of each well at 450 nm and calculate the cell proliferation rate.

[0094] (2) Effect of Platycladus orientalis leaf alcohol extract on hair growth in mice

[0095] C57BL / 6 mice are used to establish an animal model for hair loss. Hair growth in mice consists of three phases: telogen, catagen, and anagen, known as the hair growth cycle. Hair loss occurs when hair follicles transition from the telogen phase to the anagen phase. C57BL / 6 mouse hair follicles contain melanocytes. During the anagen phase, melanin production is high, resulting in a dark skin appearance. During the catagen phase, melanin production decreases, resulting in a grayish skin appearance. During the telogen phase, no melanin production occurs, resulting in a pinkish skin appearance. Hair follicles in 6-8-week-old C57BL / 6 mice enter the telogen phase, resulting in slow hair growth and a darker skin appearance on the back. The skin color and hair coverage of the mice's backs after drug administration can be used to assess whether the drug has stimulated the onset of hair growth in mice.

[0096] ① Experimental grouping

[0097] C57BL / 6 (SPF grade) male mice were randomly divided into 4 groups, with 6 mice in each group. They were kept in cages and numbered, and divided into blank group, model group, positive group and Platycladus orientalis leaf group. The model mice in the positive group were administered minoxidil, and the model mice in the Platycladus orientalis leaf group were administered Platycladus orientalis leaf alcohol extract. Deionized water was used instead of the drug in the blank group and model group, respectively.

[0098] ② Modeling

[0099] After 7 days of adaptive feeding, when the mice entered the telogen phase, C57BL / 6 mice in all groups except the blank group were anesthetized with an intraperitoneal injection of chloral hydrate (4%, w / v). The hair on the back was shaved with a razor, leaving a blank area of ​​2*2 cm to establish the model.

[0100] ③Administer medication

[0101] After establishing the model, 0.32 ml of the corresponding drug solution was applied to the backs of the mice once daily for 20 days. After drug administration, the backs of the mice were covered with plastic wrap of appropriate size to prevent drug loss. The mice were anesthetized during the application period. Hair growth on the backs of each group of mice was observed and recorded every five days during the drug administration period.

[0102] ④ Determine the coverage rate of new hair

[0103] On the 5th, 10th, 15th and 20th day of administration, the hair coverage area on the back of the mice was calculated using Image J software, and the hair coverage (%) and promotion effect EE (hair coverage) were calculated according to the following formula:

[0104] ;

[0105] .

[0106] 2. Results Analysis

[0107] (1) Analysis of human dermal papilla cell proliferation results

[0108] The results are as follows Figure 4 As shown, A shows the effect of Platycladus orientalis leaf alcohol extract on the viability of human dermal papilla cells, and B shows the effect of Platycladus orientalis leaf water extract on the viability of human dermal papilla cells, where *P<0.05, **P<0.01, ***P<0.001 represent comparisons with the blank control group; ##P<0.01, ###P<0.001 represent comparisons with the positive drug group.

[0109] It can be seen that both the alcohol extract and the water extract of Thuja orientalis leaves can significantly promote the proliferation of dermal papilla cells, which are important dermal cells for hair follicle growth. However, the proliferation effect of the alcohol extract of Thuja orientalis leaf is higher than that of the water extract of Thuja orientalis leaf at a concentration of 50µg / mL. The cell proliferation ability of the alcohol extract of Thuja orientalis leaf reaches the highest vitality at 100 µg / mL, while the cell proliferation ability of the water extract of Thuja orientalis leaf reaches the highest vitality at 200µg / mL. Moreover, the maximum activity value of the cells treated with the alcohol extract of Thuja orientalis leaf is greater than the maximum activity value of the cells treated with the water extract of Thuja orientalis leaf. Compared with the water extract of Thuja orientalis leaf, the alcohol extract of Thuja orientalis leaf has a stronger proliferation ability on dermal papilla cells.

[0110] (2) Analysis of mouse hair growth results

[0111] The changes in hair growth on the back of mice in each group on the 5th, 10th, 15th and 20th days after administration are shown in Figure 2. Figure 5 As shown in the figure, after the fifth day of treatment, there was no significant hair growth on the backs of the mice in all groups, but skin color changed. After 10 days, hair growth became more pronounced, with different groups showing varying degrees of hair growth. The Platycladus orientalis leaf group showed the most pronounced effect, indicating that the Platycladus orientalis leaf alcohol extract significantly shortens the transition time from the resting phase to the anagen phase, and the time from entering the anagen phase to hair emergence from the skin. After 20 days, compared to the model group, all groups showed hair growth on the backs of the mice, and the hair growth in the Platycladus orientalis leaf group was comparable to that in the minoxidil group. Therefore, the Platycladus orientalis leaf alcohol extract has a hair growth-promoting effect.

[0112] The back hair coverage of mice in each group on the 5th, 10th, 15th and 20th days is shown in Table 3.

[0113] Table 3

[0114]

[0115] As shown in Table 1, on day 5 of drug administration, no hair grew in the shaved areas in either the positive or the Platycladus orientalis leaf group. On day 10 of drug administration, the back skin color of the mice in the Platycladus orientalis leaf group changed. On day 15, the backs of the mice in the positive group began to darken. By day 20, most of the backs of the mice in all groups were covered with hair. The Platycladus orientalis leaf group showed a greater effect in promoting hair growth, as measured by the area of ​​newly formed hair covered. The hair coverage percentage was calculated by dividing the area of ​​newly formed hair covered by the shaved area in each group. The effect of each drug on hair restoration was evaluated by comparing the hair coverage percentage of each drug group to that of the model group on day 12 (EE). The Platycladus orientalis leaf group showed a significantly greater effect than the positive group (minoxidil group).

[0116] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Use of cubeb lactone and a Platycladus orientalis leaf extract containing cubeb lactone in the preparation of products for promoting hair growth and / or preventing hair loss; The product promotes hair growth and / or prevents hair loss by inhibiting 5α-reductase.

2. The use according to claim 1, characterized in that The Platycladus orientalis leaf extract is prepared by alcohol extraction.

3. The use according to claim 2, characterized in that The preparation method of the Platycladus orientalis leaf extract comprises the following steps: uniformly mixing Platycladus orientalis leaf coarse powder and 95% ethanol in a volume ratio of 1:12-17, soaking, ultrasonicating, and filtering to obtain the Platycladus orientalis leaf extract.

4. The use according to claim 3, characterized in that The crude arborvitae leaf powder and 95% ethanol are evenly mixed in a volume ratio of 1:

15.

5. The use according to claim 3, characterized in that The soaking time is at least 1 hour.

6. The use according to claim 3, characterized in that The ultrasonic time is 20 to 50 minutes.

7. The use according to claim 3, characterized in that The soaking, ultrasonication and filtration are performed at least twice, and the filtrates are combined after filtration to obtain the Platycladus orientalis leaf extract.

8. The use according to claim 3, characterized in that The particle size of the Platycladus orientalis leaf coarse powder is 40-60 meshes.

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