Online liquid transfer interface structure for centrifugal micro-fluidic chip

Abstract

The invention discloses a liquid online transfer interface structure for a centrifugal micro-fluidic chip. The liquid transfer interface structure comprises a static part, a rotating part and an elastic sealing ring, the static part comprises a channel connector and a first sealing ring. A sample introduction channel and a sampling channel are arranged in the channel interface; the sampling channel is positioned in the middle of the channel interface; an annular mounting groove is formed in the bottom face of the channel connector, and the bottom face of the elastic sealing ring and the top face of the first sealing ring are installed in a matched mode. The rotating component comprises a supporting layer, a second sealing ring and a fixing clamping ring. The supporting layer is arranged at the bottom of the channel interface; the second sealing ring is arranged at the top of the supporting layer and is in contact with the bottom surface of the first sealing ring; a limiting step is arranged on the outer ring of the channel interface; the bottom of the fixed clamping ring is connected with the supporting layer, and the top is lapped on the top of the limiting step; a sample introduction groove and a sampling groove are formed in positions, corresponding to the sample introduction channel and the sampling channel, of the supporting layer; a sample inlet and a sampling port are formed in the surface of the chip. The interface structure provided by the invention can be used for sampling in a centrifugal process, and can avoid sample pollution.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and more specifically, to a liquid on-line transfer interface structure for a centrifugal microfluidic chip. Background Art

[0002] Since the microfluidic technology was proposed in the 1990s, it has gradually developed towards integration and automation. Traditional microfluidic technology relies on external pumps and valves (such as air pump drive, syringe pump drive, etc.), and has problems such as large equipment volume, complex fluid control, and poor reagent compatibility, making it difficult to meet the requirements of point-of-care testing (POCT) and complex sample processing. The centrifugal microfluidic chip drives the fluid by centrifugal force without external pumps and valves. The radially distributed microchannel design enables the fluid to flow directionally from the center to the edge, realizing pulse-free and high-throughput sample processing. The centrifugal microfluidic chip has been successfully applied in the fields of platelet detection, nucleic acid extraction, coagulation analysis, digital PCR, etc.

[0003] At present, the centrifugal microfluidic chip, with its advantages of pump-free drive, high integration and low cost, has penetrated into the fields of precision medicine, biofabrication, environmental monitoring, etc. The centrifugal microfluidic chip uses centrifugal force as the driving force, and the chip rotates continuously during operation. When sampling or injecting samples into the chip, centrifugation needs to be stopped, and the sampling and injection operations are realized manually or by a robotic arm. Such operations have the following problems: ① The sampling needle is directly in contact with the air before entering the chip, and microorganisms or aerosols in the air may contaminate the sample; ② If the chip needs to be sampled or injected multiple times, the centrifugation program needs to be started and stopped multiple times, resulting in too long a sample processing time; ③ If the product after centrifugal stratification needs to be extracted, the stratified product may be remixed when centrifugation stops for sampling.

[0004] Therefore, it is an urgent problem for those skilled in the art to develop a liquid on-line transfer interface structure for a centrifugal microfluidic chip that can sample during centrifugation and avoid sample contamination. Summary of the Invention

[0005] In view of this, the present invention provides a liquid on-line transfer interface structure for a centrifugal microfluidic chip that can sample during centrifugation and avoid sample contamination.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A liquid on-line transfer interface structure for a centrifugal microfluidic chip, comprising:

[0008] Stationary component, the stationary component includes: a channel interface, a first sealing ring, and an elastic sealing ring; an injection channel and a sampling channel are provided inside the channel interface; the injection channel is located in the middle of the channel interface, the sampling channel is annular and located outside the injection channel; an annular installation groove is provided on the bottom surface of the channel interface; the first sealing ring and the elastic sealing ring are both installed in the installation groove; the elastic sealing ring is located on top of the first sealing ring, and the bottom surface of the elastic sealing ring is fitted and installed with the top surface of the first sealing ring.

[0009] Rotating component, the rotating component is arranged at the bottom of the stationary component and on top of the chip, and rotates together with the chip; the rotating component includes: a support layer, a second sealing ring, and a fixing snap ring; the support layer is placed at the bottom of the channel interface; the second sealing ring is arranged on top of the support layer and is in contact with the bottom surface of the first sealing ring; a limiting step is provided on the outer ring of the channel interface; the bottom of the fixing snap ring is connected to the support layer, and its top is lapped on top of the limiting step; sampling grooves are provided at positions corresponding to the injection channel and the sampling channel on the support layer; an injection port and a sampling port are provided on the surface of the chip, and the injection channel is communicated with the injection port through the injection groove; the sampling channel is communicated with the sampling port through the sampling groove.

[0010] The beneficial effects of adopting the above technical solution are that in the present invention, through the cooperation of the stationary component and the rotating component, the transfer of liquid can be realized during the centrifugation process, and multi-step injection and sampling operations can be completed without stopping the centrifugation, greatly shortening the time and process of chip sample processing; the sealing ring design between the stationary component and the rotating component ensures that the injection and sampling processes are carried out in a closed environment, avoiding the contact of samples and products with the external environment, thereby preventing sample contamination; this structure allows continuous operation during centrifugation, reducing the inconvenience and time waste caused by starting and stopping the centrifugation program, and improving the flexibility and efficiency of the overall operation.

[0011] Preferably, a limiting block is provided on the top of the channel interface.

[0012] Preferably, a connecting pipe is provided between the bottom of the injection channel and the injection groove. The setting of the connecting pipe makes the connection between the injection channel and the injection groove smoother, avoiding the direct contact of the liquid sample with the elastic sealing ring, the first sealing ring, the second sealing ring, etc. during injection, thereby preventing the contamination of the sample caused by these sealing materials and ensuring the purity of the sample and the accuracy of the detection results.

[0013] Preferably, a first annular channel cover and a second annular channel cover are provided between the bottom of the sampling channel and the sampling tank, and the second annular channel cover is arranged outside the first annular channel cover. A channel for the sample liquid to pass through is formed between the first annular channel cover and the second annular channel cover. The arrangement of the first annular channel cover and the second annular channel cover provides an additional isolation layer between the sampling channel and the sampling tank, so that the product obtained during sampling will not directly contact with the elastic sealing ring, the first sealing ring, the second sealing ring, etc., thereby preventing the contamination of the product caused by these sealing materials and ensuring the purity of the product and the effect of subsequent processing.

[0014] Preferably, the outer wall of the first annular channel cover is provided with protrusions.

[0015] Preferably, the bottoms of the first annular channel cover and the second annular channel cover are in an arc shape extending outward.

[0016] Preferably, the first sealing ring is a graphite sealing ring, and its main material is phenolic resin impregnated graphite material; the second sealing ring is a ceramic sealing ring, and its main material is zirconia ceramic. The first sealing ring has self-lubricity, and the second sealing ring has high mechanical strength and wear resistance. During the use of this structure, when the first sealing ring and the second sealing ring are in contact and cooperate with each other, they will rub against each other, and the mutual friction between the two sealing rings will make the sealing performance better.

[0017] Preferably, pipelines are connected to the top channel openings of the sample injection channel and the sampling channel, and an in-sampling pump is used at the end of the pipeline to perform sample injection / sampling operations into the chip.

[0018] A usage method of a liquid on-line transfer interface structure for a centrifugal microfluidic chip. By pressing down the channel interface, the elastic sealing ring deforms, so that only the first sealing ring and the second sealing ring are in contact between the stationary part and the rotating part; sample liquid is injected into the sample injection channel through the pipeline, and the sample liquid enters the chip; the position of the stationary part is limited, the rotating part rotates with the chip, and then the sample piece is processed by the chip to obtain a product. The product is taken out and collected through the pipeline at the sampling channel, passing through the sampling port, the channel between the first annular channel cover and the second annular channel cover, and the sampling channel in sequence.

[0019] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a liquid on-line transfer interface structure for a centrifugal microfluidic chip, and its beneficial effects are as follows:

[0020] (1) The present invention can perform multi-step sample injection and sampling operations under centrifugation, shortening the sample processing time and process of the chip; solving the problem that the current centrifugal microfluidic chip cannot perform sample injection and sampling operations during centrifugation;

[0021] (2) Through the design of the sealing ring between the stationary part and the rotating part, and the setting of structures such as the connecting pipe and the annular channel cover, the dynamic sealing of the sampling pipeline and the chip is realized, avoiding the pollution of the sample / product by the environment during the sampling / sampling process, ensuring the purity of the sample and the product, and improving the accuracy of the detection result;

[0022] (4) It can be further derived into single-channel, two-channel, four-channel or even more liquid transfer dynamic sealing interfaces. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to the provided drawings.

[0024] Figure 1 Schematic diagram of the internal structure of the interface structure in Embodiment 1 provided by the present invention;

[0025] Figure 2 Exploded view of the structure of the interface structure in Embodiment 1 provided by the present invention;

[0026] Figure 3 Schematic diagram of the internal structure of the connection between the interface structure and the chip in Embodiment 1 provided by the present invention;

[0027] Figure 4 Schematic diagram of the connection between the interface structure and the chip in Embodiment 1 provided by the present invention;

[0028] Figure 5 Schematic diagram of the internal structure of the connection between the interface structure and the chip in Embodiment 2 provided by the present invention;

[0029] Figure 6 Schematic diagram of the connection between the interface structure and the chip in Embodiment 2 provided by the present invention;

[0030] Figure 7 Schematic diagram of the structure of the array chip in Embodiment 3 provided by the present invention;

[0031] Figure 8 Schematic diagram of the structure of the array chip in Embodiment 4 provided by the present invention.

[0032] Among them, in the figure,

[0033] 1 - Channel interface;

[0034] 11 - Sampling channel; 12 - Sampling channel; 13 - Installation groove; 14 - Limiting step;

[0035] 2 - First sealing ring;

[0036] 3 - Chip;

[0037] 31 - Sampling port; 32 - Sampling port;

[0038] 4 - Support layer;

[0039] 41 - Sampling groove; 42 - Sampling groove;

[0040] 5 - Second sealing ring; 6 - Fixed clamping ring; 7 - Elastic sealing ring; 8 - Limiting block; 9 - First annular channel cover; 10 - Second annular channel cover; 011 - Connecting pipe. Detailed implementation mode

[0041] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0042] Embodiment 1:

[0043] Embodiment 1 of the present invention discloses a liquid on - line transfer interface structure for a centrifugal microfluidic chip, including:

[0044] Stationary components, which include: channel interface 1, first sealing ring 2 and elastic sealing ring 7; the inside of the channel interface 1 is provided with a sampling channel 11 and a sampling channel 12; the sampling channel 11 is located in the middle of the channel interface 1, the sampling channel 12 is annular, and is located outside the sampling channel 11; the bottom surface of the channel interface 1 is provided with an annular installation groove 13; both the first sealing ring 2 and the elastic sealing ring 7 are installed in the installation groove 13; the elastic sealing ring 7 is located on the top of the first sealing ring 2, and the bottom surface of the elastic sealing ring 7 is fitted and installed with the top surface of the first sealing ring 2;

[0045] The rotating component is arranged at the bottom of the stationary component and on top of the chip 3, and rotates together with the chip 3. The rotating component includes: a support layer 4, a second sealing ring 5, and a fixing snap ring 6. The support layer 4 is placed at the bottom of the channel interface 1. The second sealing ring 5 is arranged on top of the support layer 4 and is in contact with the bottom surface of the first sealing ring 2. A limiting step 14 is provided on the outer ring of the channel interface 1. The bottom of the fixing snap ring 6 is connected to the support layer 4, and its top is lapped on top of the limiting step 14. Sampling grooves 41 and 42 are provided at positions of the support layer 4 corresponding to the sample injection channel 11 and the sampling channel 12. An injection port 31 and a sampling port 32 are provided on the surface of the chip 3. The sample injection channel 11 communicates with the injection port 31 through the sampling groove 41. The sampling channel 12 communicates with the sampling port 32 through the sampling groove 42. The channel interface 1 can be made of polymer materials such as PMMA, PC, ABS, etc.

[0046] To further optimize the above technical solution, the elastic sealing ring 7 cooperates with the first sealing ring 2. When the channel interface 1 is subjected to a downward pressure, the elastic sealing ring 7 can deform so that the first sealing ring 2 is subjected to a uniform downward pressure and closely cooperates with the second sealing ring 5, making the sealing between the two more tight and further preventing the sample and the product from being contaminated during the transfer process. The main material of the elastic sealing ring 7 is elastic materials such as silicone rubber.

[0047] To further optimize the above technical solution, one sample injection channel 11 is provided, and two sampling channels 12 are provided. As Figure 1 shown, the three channels are independent of each other.

[0048] To further optimize the above technical solution, a limiting block 8 is provided at the top of the channel interface 1.

[0049] To further optimize the above technical solution, a connecting pipe 011 is provided between the bottom of the sample injection channel 11 and the sampling groove 41. The provision of the connecting pipe 011 can prevent the liquid sample from directly contacting the elastic sealing ring 7, the graphite sealing ring, and the ceramic sealing ring during sample injection, thereby contaminating the sample.

[0050] To further optimize the above technical solution, a first annular channel cover 9 and a second annular channel cover 10 are provided between the bottom of the sampling channel 12 and the sampling groove 42, and the second annular channel cover 10 covers the outside of the first annular channel cover 9. A channel for the sample liquid to pass through is formed between the first annular channel cover 9 and the second annular channel cover 10. The provision of the first annular channel cover 9 and the second annular channel cover 10 can prevent the product obtained during sampling from directly contacting the elastic sealing ring 7, the first sealing ring 2, and the second sealing ring 5, thereby contaminating the product.

[0051] To further optimize the above technical solution, protrusions are provided on the outer wall of the first annular channel cover 9. These protrusions can ensure that after the first annular channel cover 9 and the second annular channel cover 10 are sleeved, an annular channel can be formed between the two, and the formed annular channel is continuous, ensuring that liquid can pass through this annular channel.

[0052] To further optimize the above technical solution, the bottoms of the first annular channel cover 9 and the second annular channel cover 10 are in an arc shape extending outward. The annular channel formed by the first annular channel cover 9 and the second annular channel cover 10 is designed as an arc structure to ensure that product samples in the support layer 4 can be extracted during centrifugation.

[0053] To further optimize the above technical solution, the first sealing ring 2 is selected as a graphite sealing ring; the second sealing ring 5 is selected as a ceramic sealing ring. The main material of the graphite sealing ring is phenolic resin impregnated graphite material; the main material of the ceramic sealing ring is zirconia ceramic material.

[0054] To further optimize the above technical solution, under the action of the downward pressure, the only contact and friction between the stationary part and the rotating part occur between the graphite sealing ring of the stationary part and the ceramic sealing ring of the rotating part. The relative friction between the graphite sealing ring and the ceramic sealing ring ensures the sealing performance of the entire dynamic rotary sealing structure. The ceramic sealing ring can maintain the flatness of the sealing surface under high-speed and high-friction working conditions. When rubbing with the graphite sealing ring, the self-lubricity of graphite can enhance the sealing performance of the rotating contact surface.

[0055] To further optimize the above technical solution, pipelines are connected to the top channel openings of the sample injection channel 11 and the sampling channel 12, and a sample injection / sampling pump is used at the end of the pipeline to perform sample injection / sampling operations on the chip 3. The sample injection / sampling pump can be a syringe, an injection pump, a peristaltic pump, a pressure pump, etc.

[0056] A usage method of a liquid on-line transfer interface structure for a centrifugal microfluidic chip. By pressing down on the channel interface 1, the elastic sealing ring 7 deforms, so that only the first sealing ring 2 and the second sealing ring 5 are in contact between the stationary part and the rotating part; sample liquid is injected into the sample injection channel 11 through a syringe, and the sample liquid enters the chip 3; the position of the stationary part is limited, the rotating part rotates with the chip 3, and then the sample is processed by the chip 3 to obtain a product. The product is taken out and collected through the syringe at the sampling channel 12 after passing through the sampling port 32, the channels of the first annular channel cover 9 and the second annular channel cover 10, and the sampling channel 12 in sequence from the chip 3.

[0057] As Figure 3 and Figure 4As shown in the figure, the bottom of the support layer 4 structure and the top of the chip 3 are glued together with glue as a whole, and the sample injection groove 41 of the support layer 4 is connected to the sample injection port 31 of the chip 3, and the two sampling grooves 42 are respectively connected to the two sampling ports 42 of the chip 3. Place the whole structure on the centrifugal platform. The stationary part of the rotary sealing structure can be fixed by the limit block 8 on the channel interface 1. Apply a certain downward pressure to the channel interface 1, and the sample injection channel 11 is communicated with the sample injection port 31, and the two sampling channels are communicated with the sampling port 32. At this time, the centrifugal platform starts to rotate centrifugally. The stationary part and the connected syringe do not rotate, and the rotating part and the chip 3 rotate driven by the centrifugal platform. Push the syringe to inject the sample from the sample injection channel 11 and the connecting tube 011 into the sample injection port 31 of the chip 3. Then the sample is processed by the centrifugal microfluidic chip to obtain product A and product B. Pull the syringe connected to the sampling channel 12 at the product A position to extract product A from the chip 3 through the sampling port 32 at the product A position and the sampling channel 12 at the product A position into the syringe for collection; similarly, pull the syringe connected to the sampling channel 12 at the product B position to extract product B from the chip 3 through the sampling port 32 at the product B position and the sampling channel 12 at the product B position into the syringe for collection. At this time, the centrifugation has not stopped and the sample injection and product extraction have been completed. During the sample injection process, the sample and the product are carried out in a closed environment. In this embodiment, the rotary sealing structure is used to realize the operation of injecting samples from one port and sampling from two ports during the centrifugation process.

[0058] Embodiment 2:

[0059] As Figure 5 、 6 shown in the figure, the rotary sealing structure has two channels, that is, it has a sample injection channel 11 and a sampling channel 12. The sample injection channel 11 is connected to the sample injection port 31 of the chip 3, and the sampling channel 12 is connected to the sampling port 42 of the chip 3. Place the whole structure on the centrifugal platform. The stationary part of the rotary sealing structure can be fixed by the limit block 8 on the channel interface 1. Apply a certain downward pressure to the channel interface 1, and the sample injection channel 11 is communicated with the sample injection port 31, and the sampling channel 12 is communicated with the sampling port 32. At this time, the centrifugal platform starts to rotate centrifugally. The stationary part and the connected syringe do not rotate, and the rotating part and the chip 3 rotate driven by the centrifugal platform. Push the syringe to inject the sample from the sample injection channel 11 and the connecting tube 011 into the sample injection port 31 of the chip 3. Then the sample is processed by the centrifugal microfluidic chip to obtain the product. Pull the syringe connected to the sampling channel 12 at the product position to extract the product from the chip 3 through the sampling port 32 at the product position and the sampling channel 12 at the product position into the syringe for collection. At this time, the centrifugation has not stopped and the sample injection and product extraction have been completed. During the sample injection process, the sample and the product are carried out in a closed environment. In this embodiment, the rotary sealing structure is used to realize the operation of injecting samples from one port and sampling from one port during the centrifugation process.

[0060] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be elaborated one by one here.

[0061] Embodiment 3:

[0062] The previous embodiments mainly illustrate the operation cases of the dynamic sealing structure in cooperation with a single-structure microfluidic chip (that is, a microfluidic chip has only one fluid control structure, one sample injection port, and one or two sampling ports). This embodiment mainly introduces the operation cases of the injection / sampling during the centrifugation process after the rotary sealing structure of two channels is combined with an array centrifugal microfluidic chip. As Figure 7 The figure shows in detail the number and positions of the injection / sampling ports of the array chip. The array chip has four identical structures and is symmetrically distributed around the central origin (the detailed structure is not drawn in the figure, only the injection / sampling ports are shown). The array chips share the injection port 31, while their respective sampling ports 32 are independent. However, since the sampling channel 12 is an annular chamber, it can completely include these four independent sampling ports 32. Place the entire structure on the centrifugation platform. The stationary part of the rotary sealing structure can be fixed by the limit block 8 on the channel interface 1. Apply a certain downward pressure to the channel interface 1, so that the injection channel 11 is connected to the injection port 31, and the sampling channel 12 is connected to the sampling port 32. At this time, the centrifugation platform starts to rotate for centrifugation. The stationary part and the connected syringe do not rotate, while the rotating part and the chip 3 rotate driven by the centrifugation platform. Push the syringe to introduce the sample from the injection channel 11 and the connecting tube 011 into the injection port 31 of the chip 3. The sample will enter the four fluid control structures in the array chip for processing at the same time. Then the sample is processed by the centrifugal microfluidic chip to obtain the product. Pull the syringe connected to the sampling channel 12 at the product position to extract the product from the array chip through the four identical sampling ports 32 and the sampling channel 12 at the product position into the syringe for collection. At this time, the centrifugation has not stopped and the sample injection and product extraction have been completed. During the injection / sampling process, the sample and the product are carried out in a closed environment.

[0063] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be elaborated one by one here.

[0064] Embodiment 4:

[0065] This embodiment mainly introduces the operation cases of the injection / sampling during the centrifugation process after the rotary sealing structure of three channels is combined with an array centrifugal microfluidic chip. As Figure 8The figure shows in detail the number and positions of the sampling inlets of the array chip. The array chip has four identical structures that are symmetrically distributed around the central origin (the detailed structure is not shown in the figure, only the in / sampling inlets are shown). The array chip shares the injection port 31, while the respective sampling ports 32 are independent. However, since both sampling grooves 12 are circular ring-shaped chambers, the channel of the first sampling groove 12 can completely enclose the four identical sampling ports 32, and similarly, the channel of the second sampling groove 12 can completely enclose the four identical sampling ports 32. Placing the entire structure on a centrifuge platform, the stationary part of the rotary sealing structure can be fixed by the limit block 8 on the fixed channel interface 1. Applying a certain downward pressure to the channel interface 1, the injection channel 11 is connected to the injection port 31, and the two sampling channels are connected to the sampling ports 32. At this time, the centrifuge platform starts to rotate for centrifugation. The stationary part and the connected syringe do not rotate, while the rotating part and the chip 3 rotate driven by the centrifuge platform. By pushing the syringe, the sample enters the injection port 31 of the chip 3 from the injection channel 11 and the connecting tube 011. The sample will simultaneously enter the four fluid control structures in the array chip for processing. Then the sample is processed by the array centrifugal microfluidic chip to obtain product A and product B. By pulling the syringe connected to the sampling channel 12 at the product A position, product A is extracted from the array chip through the four identical sampling ports 32 at the product A position and the sampling channel 12 at the product A position into the syringe for collection; similarly, by pulling the syringe connected to the sampling channel 12 at the product B position, product B is extracted from the array chip through the four identical sampling ports 32 at the product B position and the sampling channel 12 at the product B position into the syringe for collection. At this time, the centrifugation has not stopped and the sample injection and product extraction have been completed. During the sampling process, the sample and the product are carried out in a closed environment.

[0066] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be elaborated here one by one.

[0067] The above-described embodiments are only a part of the application cases of the rotary sealing liquid transfer interface structure. More practical applications depend on the fluid control functions or the purposes to be achieved by the designed centrifugal microfluidic chip (such as fluid mixing, fluid separation, fluid reaction, etc.). The embodiment emphasizes more that the rotary sealing liquid transfer interface structure can realize the liquid transfer function of one to multiple independent channels during centrifugal rotation. All structural designs that increase or decrease the sealing channels on this structure are within the scope of this patent protection.

[0068] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. The same or similar parts among the various embodiments can be referred to each other.

[0069] The foregoing description of the disclosed embodiments enables those skilled in the art to practice or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A liquid online transfer interface structure for a centrifugal microfluidic chip, characterized in that: include: A stationary component, the stationary component comprising: a channel interface (1), a first sealing ring (2) and an elastic sealing ring (7); an injection channel (11) and a sampling channel (12) are arranged inside the channel interface (1); the injection channel (11) is located in the middle of the channel interface (1), and the sampling channel (12) is ring-shaped and is located at the periphery of the injection channel (11); an annular mounting groove (13) is arranged on the bottom surface of the channel interface (1); the first sealing ring (2) and the elastic sealing ring (7) are both installed in the mounting groove (13); the elastic sealing ring (7) is located on the top of the first sealing ring (2), and the bottom surface of the elastic sealing ring (7) is installed in coordination with the top surface of the first sealing ring (2); A rotating component, the rotating component is arranged at the bottom of the stationary component and at the top of the chip (3), and rotates together with the chip (3); the rotating component comprises: a supporting layer (4), a second sealing ring (5) and a fixing snap ring (6); the supporting layer (4) is placed at the bottom of the channel interface (1); the second sealing ring (5) is arranged on the top of the supporting layer (4) and contacts the bottom surface of the first sealing ring (2); the outer ring of the channel interface (1) is provided with a limiting step (14); the fixing snap ring The bottom of the chip (6) is connected to the support layer (4), and the top of the chip (6) is overlapped with the top of the limiting step (14); the support layer (4) is provided with a sampling groove (41) and a sampling groove (42) at positions corresponding to the sampling channel (11) and the sampling channel (12); the surface of the chip (3) is provided with a sampling port (31) and a sampling port (32); the sampling channel (11) is connected to the sampling port (31) through the sampling groove (41); the sampling channel (12) is connected to the sampling port (32) through the sampling groove (42).

2. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that: A limit block (8) is arranged on the top of the channel interface (1).

3. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that: A connecting tube (011) is provided between the bottom of the injection channel (11) and the injection slot (41).

4. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that: A first annular channel cover (9) and a second annular channel cover (10) are arranged between the bottom of the sampling channel (12) and the sampling groove (42), and the second annular channel cover (10) is arranged outside the first annular channel cover (9), and a channel for the sample liquid to pass through is formed between the first annular channel cover (9) and the second annular channel cover (10).

5. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 4, characterized in that: The outer wall of the first annular channel cover (9) is provided with a protrusion.

6. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 4, characterized in that: The bottoms of the first annular channel cover (9) and the second annular channel cover (10) are in an arc shape extending outwards.

7. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that: The first sealing ring (2) is a graphite sealing ring; the second sealing ring (5) is a ceramic sealing ring.

8. The liquid online transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that: The top channel openings of the injection channel (11) and the sampling channel (12) are both connected to pipelines, and an injection / sampling pump is used at the end of the pipeline to implement the injection / sampling operation on the chip (3).

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