A detection device for dairy gold-labeled immunochromatography

Abstract

The present invention relates to a detection device for gold-labeled immunochromatography of dairy products. A movably placed sample rack is provided above a swing rack. The holes of the sample rack correspond one-to-one to the holes of the swing rack. The middle and lower parts of sample tubes are inserted into the holes of the swing rack. A sampler can penetrate the sealing membrane of the sample tube to input dairy products into the sample tube. The swing rack leads the middle and lower parts of the sample tube to swing, and the gold-labeled antibody and the sample are evenly mixed. The sampler and the sealer can move along a three-dimensional movable rack. The adsorption plate of the sealer can absorb the sealing cover on the incubation pool and buckle it on the top of the sample tube. The clamping forceps can clamp the sample rack into the incubation pool for incubation. A first conveyor belt and a detector are provided in the detection area. The sampler drips a mixed sample liquid onto the detection card on the first conveyor belt. The detector detects the chromatography test paper on the detection card to determine the target content in the dairy sample. The present invention solves the problem that the existing gold-labeled antibody chromatography detection card requires a heterogeneous reaction between the sample and the gold-labeled antibody, resulting in insufficient reaction and low sensitivity.

Description

Technical Field

[0001] The invention belongs to the technical field of dairy gold-labeled immunochromatography, and particularly relates to a detection device for dairy gold-labeled immunochromatography. Background Art

[0002] Dairy products are an important part of people's daily diet, and the quality and safety of dairy products are extremely important. The detection of harmful targets in dairy products is still mainly based on manual operation of colloidal gold test strips. In traditional colloidal gold test card detection technology, the gold-labeled antibody is fixed to the release pad of the test card. After the dairy sample is dripped into the sample addition hole of the test card, the liquid flows through the release pad and directly carries the gold-labeled antibody to the detection line position, where it undergoes a color reaction with the antigen. The target in the dairy sample reacts heterogeneously with the gold-labeled antibody, resulting in an insufficient reaction and low sensitivity. In addition, traditional colloidal gold detection technology requires a large number of professional and technical personnel, and the uncertainty factors introduced by manual operation may affect the accuracy of the test results. Summary of the Invention

[0003] In response to the above problems, the present invention provides a detection device for gold-labeled immunochromatography of dairy products, comprising a three-dimensional movable rack, a sample preparation area, and a detection area. The sample preparation area comprises a sampler, a sealer, a swing rack, and an incubation pool. Several swing racks are arranged in a row, and a movably placed sample rack is provided above the swing rack for accommodating several sample tubes. The holes of the sample rack correspond one-to-one with the holes of the swing rack. The middle and lower parts of the sample tubes are inserted into the corresponding holes on the swing rack. The sampler can penetrate the sealing film of the sample tube, and the dairy sample is input into the sample tube. The swing rack leads the middle and lower parts of the sample tubes to swing back and forth, so that the gold-labeled antibody and the sample are evenly mixed.

[0004] The sampler and sealer can move along the three-dimensional movable rack; the sealer includes an adsorption plate and a clamping forceps. The adsorption plate can absorb the sealing cover on the incubation pool and buckle it on the top of the sample tube. The clamping forceps can clamp the sample rack into the incubation pool for incubation;

[0005] The detection area is equipped with a first conveyor belt and a detector. The sampler absorbs the mixed liquid in the incubated sample tube and drops it onto the detection card on the first conveyor belt. The detector is used to detect the chromatography test paper on the detection card to determine the target content in the dairy sample.

[0006] Optionally, the swing frame is a frame structure with a plurality of holes on the top, the holes being evenly arranged along the length of the swing frame, the bottom surface of the swing frame being slidably connected to a bottom rail below, the plurality of bottom rails corresponding to a plurality of swing frames being arranged in a row;

[0007] At least one driving device is provided on the side of the rocking frame. The driving device is connected to one end of the rocking frame through a rod and drives the rocking frame to move back and forth along the bottom track.

[0008] Optionally, the sampler includes an air pump and a sampling part that are connected to each other, the air pump is connected to an external air supply device to supply air or absorb air to the sampling part, and the bottom opening of the sampling part is used to connect a pipette tip.

[0009] Optionally, the sample tube includes a support portion and a container portion, the support portion is detachably mounted on the top of the container portion, the support portion is made of hard plastic material, and is cylindrical, with openings at the top and bottom, and is used to support the sample tube on the hole of the sample rack; the container portion is made of a flexible material, such as soft rubber, and is conical in shape with a larger top and a smaller bottom, and can be inserted into the hole of the swing rack and moved by the swing rack.

[0010] Further optionally, the gold-labeled antibody is freeze-dried and placed in the container part, and the top of the support part is heat-sealed with aluminum film to effectively isolate the gold-labeled antibody from moisture in the environment, which is beneficial to the long-term storage of the gold-labeled antibody. The pipette tip pierces the aluminum film to input the dairy sample into the sample tube.

[0011] Optionally, the sealer includes an electric controller, a controller, and a lifting hammer, wherein the electric controller is electrically connected to an external power supply and an adsorption plate. After the adsorption plate is powered, the adsorption plate generates a magnetic attraction force to attract the sealing cap to seal the sample tube;

[0012] The two grippers of the clamping clamp are respectively arranged on both sides of the adsorption plate, and can move closer to or away from each other to clamp or release the two ends of the sample rack; the lifting hammer can move along its own track and perform lifting actions at the same time. The lifting hammer hits the adsorption plate and can buckle the sealing cover on the tube mouth of the sample tube; the controller connects and controls the actions of the lifting hammer and the clamping clamp.

[0013] Further optionally, a driver is provided on the top of the lifting hammer for controlling the up and down movement of the lifting hammer; the driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

[0014] Optionally, the sealing cover is a hollow plastic circle, the bottom of the circle can be connected to the top of the sample tube, and the top of the circle is connected to fixed plate one. The part of fixed plate one corresponding to the sealing cover is hollowed out, and fixed plate one and the sealing cover are integrally formed; fixed plate two is provided above fixed plate one, and a plastic film is sandwiched between fixed plate two and fixed plate one, so that the plastic film can cover the tube mouth of the sample tube; at least one magnetic block is provided on the upper surface of fixed plate two.

[0015] Optionally, the detection area is provided with a card storage bin, a first conveyor belt and a second conveyor belt from top to bottom. The card storage bin is vertical, and a number of detection cards are stacked inside. The first conveyor belt and the second conveyor belt are both horizontal. A conveying part is provided between the first conveyor belt and the second conveyor belt for conveying the detection cards. The outer cover of the second conveyor belt is provided with a heating bin for incubating the detection cards dripped with the mixed liquid to make them react better, and then perform chromatography and color development. A detector is provided at the tail of the second conveyor belt.

[0016] Further optionally, a plurality of positioning frames are provided on the first conveyor belt, the positioning frames protruding from the upper surface of the first conveyor belt, the plurality of positioning frames are evenly arranged along the moving direction of the first conveyor belt and arranged in a straight line, and the positioning frames are used to clamp the test card, so that the sampler can determine the position of the test card;

[0017] The positioning frame is a square with three sides. The opening of the positioning frame faces the opposite direction of the movement of the first conveyor belt, and the opening of the positioning frame on the upper surface of the first conveyor belt faces the card storage bin. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of the detection device for the gold-labeled immunochromatographic analysis of dairy products;

[0019] Figure 2 for Figure 1 A side view schematic diagram of

[0020] Figure 3 is a schematic diagram of the sample tube;

[0021] Figure 4 is a schematic diagram of the locator;

[0022] Figure 5 It is a schematic diagram of the cooperation between the sealing cover and the fixing plate 1 and the fixing plate 2.

[0023] In the accompanying drawings, 1-sampler, 2-sealer, 3-swing rack, 4-heating chamber, 5-positioning frame, 6-sample tube, 7-adsorption plate, 8-gripping forceps, 9-first conveyor belt, 10-second conveyor belt, 11-detector, 12-detection card, 13-X-axis moving track, 14-Y-axis moving track, 15-Z-axis moving track, 16-slide rail, 17-telescopic rod, 18-positioning half frame, 19-pipette tip, 20-bottom track, 21-driving device, 22-air pump, 23-sampling part, 24-support part, 25-container part, 26-lifting hammer, 27-housing, 28-sealing cover, 29-fixed plate one, 30-fixed plate two, 31-card storage bin, 32-transport part. DETAILED DESCRIPTION

[0024] This embodiment provides a detection device for gold-labeled immunochromatography of dairy products, such as Figure 1-Figure 5As shown, it includes a three-dimensional mobile rack, a sample preparation area and a detection area. The sample preparation area includes a sampler 1, a sealer 2, a swing rack 3 and an incubation pool. Several swing racks 3 are arranged in a row. A movable sample rack is provided above the swing rack 3 for placing several sample tubes 6. The holes of the sample rack correspond one-to-one with the holes of the swing rack 3. The middle and lower parts of the sample tubes 6 are inserted into the corresponding holes on the swing rack 3. The sampler 1 can penetrate the sealing film of the sample tube 6 to input the dairy sample into the sample tube 6. The swing rack 3 leads the middle and lower parts of the sample tube 6 to swing back and forth to evenly mix the gold-labeled antibody with the sample.

[0025] The sampler 1 and the sealer 2 can move along the three-dimensional movable frame; the sealer 2 includes an adsorption plate 7 and a clamping forceps 8. The adsorption plate 7 can absorb the sealing cover 28 on the incubation pool and buckle it on the top of the sample tube 6. The clamping forceps 8 can clamp the sample rack into the incubation pool for incubation;

[0026] A first conveyor belt 9 and a detector 11 are provided in the detection area. The sampler 1 absorbs the mixed liquid in the incubated sample tube 6 and drops it onto the detection card 12 on the first conveyor belt 9. The detector 11 is used to detect the chromatography test paper on the detection card 12 to determine the target content in the dairy sample.

[0027] The detection device of the present invention primarily performs two functions: sample preparation and detection. Specifically, a gold-labeled antibody is placed in advance into a sample tube 6 and then sealed. A sampler 1 is inserted into the tube, and a dairy sample is introduced into the tube. The dairy and gold-labeled antibody then react within the tube. To facilitate this reaction, a rocking frame 3 and an incubation tank are provided. The rocking frame 3 drives the lower portion of the sample tube 6 to swing, promoting uniform mixing of the dairy and gold-labeled antibody for a more complete reaction. The presence of the sample tube 6 within the incubation tank, at a certain temperature, further promotes the reaction.

[0028] However, before the sample tube 6 is sent into the incubation tank, it needs to be sealed again to protect it from environmental influences. However, the sampler 1 has already punctured the seal, so how to quickly and easily seal the mouth of the sample tube 6 again is a problem. The present invention designs a sealer 2, whose adsorption plate 7 can magnetically attract the sealing cover 28 and then buckle it on the top of the sample tube 6 to achieve a simple seal. Finally, the sampler 1 punctures the simple seal again, absorbs the mixture of dairy products and gold-labeled antibodies, and then drips it onto the test card for chromatography. Finally, the detector 11 quantitatively detects the target content in the dairy sample.

[0029] Optionally, the three-dimensional movable frame includes an X-axis movable rail 13, a Y-axis movable rail 14 and a Z-axis movable rail 15. The X-axis movable rail 13 is arranged on the side of the sample preparation area and the detection area, the bottom of the Y-axis movable rail 14 is slidably connected to the X-axis movable rail 13, one end of the Z-axis movable rail 15 is slidably connected to the Y-axis movable rail 14, and the other end extends in a direction away from the X-axis movable rail 13; the sampler 1 and the sealer 2 are slidably connected to the Z-axis movable rail 15, and can move within the sample preparation area and the detection area under the control of the three-dimensional movable frame.

[0030] Optionally, the sample preparation area is divided into several areas for placing consumables racks, dairy sample racks, rocking racks 3, incubation pools, incubated sample racks and waste boxes respectively. The sampler 1 and the sealer 2 move between the areas under the control of the three-dimensional mobile rack; the sample rack, consumables rack and dairy sample rack are all conventional multi-porous test tube racks or sampling tube racks, the consumables rack is used to place multiple pipette tips 19, the dairy sample rack is used to place multiple sample tubes individually filled with dairy products, and the waste box is used to hold used pipette tips 19.

[0031] Optionally, the swing frame 3 is a frame structure with a plurality of holes on the top, the holes being evenly arranged along the length of the swing frame 3, the bottom surface of the swing frame 3 being slidably connected to the bottom rail 20 below, and the plurality of bottom rails 20 corresponding to the plurality of swing frames 3 and arranged in a row;

[0032] At least one driving device 21 is provided on the side of the swing frame 3 . The driving device 21 is connected to one end of the swing frame 3 via a rod, and drives the swing frame 3 to move back and forth along the bottom track 20 .

[0033] As a specific embodiment, the swing frame 3 is a rectangular parallelepiped frame structure with two layers, the top surface of the upper layer being evenly provided with at least one row of holes for inserting the middle and lower portions of the sample tubes 6. The lower surface of the bottom surface of the lower layer is slidably connected to the bottom track 20. A drive device 21 is provided on the side of the swing frame 3. The drive device 21 is connected in parallel to the end of each swing frame 3 near the drive device 21 through several rods. The rods are extended and retracted to drive the swing frame 3 toward or away from the drive device 21, thereby causing the middle and lower portions of the sample tubes 6 to swing back and forth, evenly mixing the gold-labeled antibodies inside with the sample. The number of swing frames 3 used is selected based on the number of sample tubes 6. For swing frames 3 that are not in use, the corresponding rods are removed, that is, the drive device 21 is disconnected.

[0034] Alternatively, a plurality of driving devices 21 are provided on the side of the swing frame 3, and the driving device 21 is connected to the end of the swing frame 3 close to the driving device 21 through a rod. The driving device 21 corresponds to the swing frame 3 one by one to realize the independent driving of the swing frame 3.

[0035] Optionally, the sample rack is a single-layer rack with only one horizontal plate, and a number of holes are evenly arranged on the plate for placing sample tubes 6. The horizontal plate is above the swing rack 3, and the holes of the sample rack correspond to the holes of the swing rack 3 up and down, so that the sample tubes 6 pass through the corresponding holes of the sample rack and the holes of the swing rack 3; the sample rack is placed across all the swing racks 3 and the bottom track 20.

[0036] Optionally, the sampler 1 includes an air pump 22 and a sampling portion 23 connected to each other, the air pump 22 is connected to an external air supply device to supply or absorb air to the sampling portion 23, and the bottom opening of the sampling portion 23 is used to connect the pipette tip 19. The air pump 22 is an existing air plunger pump.

[0037] Before use, place the sample rack above the rocking rack 3, insert the number of sample tubes 6 required for testing into the corresponding holes of the sample rack and the rocking rack 3, and place the sample tubes 6 compactly, using the least number of rocking racks 3. Then, pack the different dairy samples into the corresponding sample tubes, and then place them on the dairy sample rack. When in use, the sampler 1 moves along the three-dimensional movable rack to the top of the consumables rack, and then descends. A pipette tip 19 is plugged into the bottom of the sampling part 23; the sampler 1 is then moved to the dairy sample rack, and the dairy product is sucked under the control of the air pump 22. Then, the sampler 1 moves to the top of the rocking rack 3, and when it descends, the pipette tip 19 pierces the sealing film at the mouth of the sample tube 6, and the air pump 22 pumps the dairy product into the sample tube 6. The sampler 1 is then moved to a waste bin. The top of the bin has an inwardly projecting sidewall that catches the top edge of a pipette tip 19. The sampler 1 is then moved upward, and the pipette tip 19 is removed, falling into the bin. After all sample tubes 6 of a rocking rack 3 have been loaded, the drive device 21 of the rocking rack 3 moves the rack 3 to mix the milk product with the gold-labeled antibody.

[0038] Optionally, the sample tube 6 includes a support portion 24 and a container portion 25. The support portion 24 is detachably mounted on the top of the container portion 25. The support portion 24 is made of hard plastic and is cylindrical with openings at the top and bottom, for supporting the sample tube 6 on the hole of the sample rack; the container portion 25 is made of a flexible material, such as soft rubber, and is conical in shape with a larger top and a smaller bottom, and can be inserted into the hole of the swing rack 3 and moved by the swing rack 3.

[0039] Further optionally, the top of the container portion 25 is provided with a circle of outwardly protruding portions, and the bottom of the support portion 24 is also provided with a circle of outwardly protruding portions, and the outwardly protruding portions of the support portion 24 and the container portion 25 are mutually engaged to achieve connection between the two;

[0040] After freeze-drying, the gold-labeled antibody is placed in the container part 25, and the top of the support part 24 is heat-sealed with aluminum film to effectively isolate the gold-labeled antibody from moisture in the environment, which is beneficial to the long-term storage of the gold-labeled antibody. The pipette tip 19 pierces the aluminum film to input the dairy sample into the sample tube 6.

[0041] When a large number of sample tubes 6 are required for one test, the plastic tops of the support parts 24 of the sample tubes 6 can be made to be connected, that is, the support parts 24 of the sample tubes 6 are connected into one piece, so that multiple sample tubes 6 can be taken at the same time.

[0042] The conventional sample tube 6 is made of hard plastic and is integrally formed, with a lid that can be opened and closed at the top, but the sealing performance of the lid is average. In order to promote the full reaction between the dairy product and the gold-labeled antibody, the present invention freeze-dries a certain amount of the gold-labeled antibody to form a lyophilized powder, places it in the sample tube 6, and stores it until it is used during testing. While the sealing performance of the conventional sample tube 6 lid cannot meet the requirements for preserving the lyophilized powder, the present invention uses aluminum film heat sealing technology, which is applicable to the support portion 24 of plastic material, has a good sealing effect, and is easy to operate.

[0043] The present invention also considers placing the milk product in the sample tube 6 and then directly pumping it with the air pump 22 to mix the milk product and the gold-labeled antibody within the sample tube 6 and the pipette tip 19. However, this method would cause the mixed solution to vibrate violently, which could easily cause splashing and material loss. If unreacted milk product or antigen is lost, it would directly lead to inaccurate quantitative detection. In addition, the mixed solution could easily splash or stick to the sampling portion 23, contaminating the next sample.

[0044] The sample tube 6 of the present invention is divided into two detachable parts, one above the other, and has low raw material costs, is simple to manufacture, and can be mass-produced. The connection and assembly of the support portion 24 and the container portion 25 are simple. The support portion 24 provides sufficient strength for the sample tube 6 and can be snap-fitted and supported on the hole of the sample rack. The container portion 25 is made of a flexible material and is inserted into the hole of the swing frame 3. The container portion 25 can swing back and forth with the swing frame 3 to promote mixing of the dairy product and the gold-labeled antibody. The movement of the swing frame 3 is controlled by the drive device 21 and is controllable, preventing violent oscillation of the mixed solution. The mixed solution is mixed and incubated only within the sample tube 6, without contaminating other components, thus avoiding contamination and improving the accuracy of the test.

[0045] Optionally, the sealer 2 includes an electric controller, a controller, and a lifting hammer 26. The electric controller is electrically connected to an external power supply and the adsorption plate 7. After the adsorption plate 7 is powered, the adsorption plate 7 generates a magnetic attraction force, which can attract the sealing cap 28 to seal the sample tube 6.

[0046] The two grippers of the clamping clamp 8 are respectively arranged on both sides of the adsorption plate 7, and can move closer to or away from each other to clamp or release the two ends of the sample rack; the lifting hammer 26 can move along its own track and perform lifting actions at the same time. The lifting hammer 26 hits the adsorption plate 7 and can buckle the sealing cover 28 on the tube mouth of the sample tube 6; the controller connects and controls the actions of the lifting hammer 26 and the clamping clamp 8.

[0047] Optionally, the sealer 2 includes a housing 27, one side of which is slidably connected to the Z-axis moving track 15. The electronic controller and the controller are located within the housing. The suction plate 7 is located below the housing. The gripping clamp 8 is connected to the bottom of the housing. The bottom of the housing is provided with a self-propelled track along which the lifting hammer 26 can move. The suction plate 7 is suspended below the housing by several connecting rods.

[0048] Further optionally, the gripping forceps of the clamping forceps 8 have two position gears, one gear is close to the adsorption plate 7, and the other gear is away from the adsorption plate 7. When both gripping forceps are close to the adsorption plate 7, the sample rack can be grabbed; when both gripping forceps are away from the adsorption plate 7, the sample rack can be released, and the distance between the two gripping forceps is greater than the length or width of the sample rack to avoid the gripping forceps touching the sample rack at this time; the bottom of the gripping forceps is lower than the adsorption plate 7.

[0049] Further optionally, the adsorption plate 7 is a flat plate, and its area is slightly smaller than the area of the top surface of the sample rack, but can cover all the holes of the sample rack.

[0050] Further optionally, a driver is provided on the top of the lifting hammer 26 for controlling the up and down movement of the lifting hammer 26; the driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

[0051] Optionally, the sealing cover 28 is a hollow plastic circle, the bottom of the circle can be connected to the top of the sample tube 6, and the top of the circle is connected to the fixing plate 1 29. The part of the fixing plate 1 29 corresponding to the sealing cover 28 is hollow, and the fixing plate 1 29 and the sealing cover 28 are integrally formed; a fixing plate 2 30 is provided above the fixing plate 1 29, and a plastic film is sandwiched between the fixing plate 2 30 and the fixing plate 1 29, so that the plastic film can cover the corresponding tube mouth of the sample tube 6; at least one magnetic block is provided on the upper surface of the fixing plate 2 30.

[0052] The second fixing plate 30, the first fixing plate 29 and the plastic film are detachable, the two fixing plates can be reused, and the second fixing plate 30, the first fixing plate 29 and the plastic film are packaged to facilitate overall movement.

[0053] During use, after the mixed liquid in the sample tube 6 is mixed, the sealer 2 moves to the top of the incubation pool. The incubation pool is covered with a cover plate, on which a sealing cover 28 and two fixing plates are placed. The adsorption plate 7 is energized to suck up the fixing plate 2 30. The sealer 2 is then moved to the top of the sample rack, and the two fixing plates are placed on the top of the sample rack. The sealing cover 28 corresponds to the top of the sample tube 6 one by one. The lifting hammer 26 moves along its own track to the position corresponding to each sample tube 6 in turn. The lifting hammer 26 hits the adsorption plate 7 vertically downward in turn, indirectly acting on the two fixing plates and the sealing cover 28, so that the sealing cover 28 is engaged with the corresponding sample tube 6. At this time, the two fixing plates are connected to the sample tube 6 through the sealing cover 28.

[0054] The sample rack is gripped by the gripping forceps 8 and moved into the incubation tank (the cover has been removed in advance). The sealer 2 is then removed from the incubation tank. The incubation tank provides a metal bath at a constant temperature, promoting the reaction between the milk product and the gold-labeled antibody in the sample tube 6. After incubation, the sealer 2 removes the sample rack and places it in the sample preparation area. The incubation tank is then covered again. The retaining plate 2 30 is removed, exposing the plastic film. The sampler 1 pierces the film and samples are taken from the sample tube 6.

[0055] Optionally, the detection area is provided with a card storage bin 31, a first conveyor belt 9 and a second conveyor belt 10 from top to bottom, the card storage bin 31 is vertical, and a plurality of detection cards 12 are stacked inside; the first conveyor belt 9 and the second conveyor belt 10 are both horizontal, and a conveying part 32 is provided between the first conveyor belt 9 and the second conveyor belt 10 for conveying the detection cards; the outer cover of the second conveyor belt 10 is provided with a heating bin 4 for incubating the detection cards dripped with the mixed liquid to make them react better, and then chromatograph and color develop; a detector 11 is provided at the tail of the second conveyor belt 10.

[0056] Further optionally, the card storage bin 31 is a rectangular parallelepiped with a push portion at the top and an opening at the bottom. Manually pressing the push portion downward can push the bottom test card to fall from the bottom opening onto the first conveyor belt 9.

[0057] Further optionally, a plurality of positioning frames 5 are provided on the first conveyor belt 9, the positioning frames protruding from the upper surface of the first conveyor belt, and the plurality of positioning frames 5 are evenly arranged along the moving direction of the first conveyor belt 9 and arranged in a straight line. The positioning frames 5 are used to clamp the test card, so that the sampler 1 can determine the position of the test card.

[0058] The positioning frame 5 is a square with three sides. The opening of the positioning frame 5 faces the opposite direction of movement of the first conveyor belt 9 , and the opening of the positioning frame 5 on the upper surface of the first conveyor belt 9 faces the card storage bin 31 .

[0059] Further optionally, a positioner is provided below the card storage bin 31, the positioner comprising two slide rails 16 and two retractable positioning claws, the positioning claws being slidably connected to the slide rails 16, the slide rails 16 being parallel to the first conveyor belt 9, and the two slide rails 16 and the positioning claws being symmetrically arranged with the first conveyor belt 9 as the center line;

[0060] The positioning claw includes a telescopic rod 17 and a positioning half frame 18. The positioning half frame 18 is connected to the slide rail 16 through the telescopic rod 17. The positioning half frame 18 is L-shaped. The shape formed by the two symmetrical positioning half frames 18 is the same as the positioning frame 5, which is used to frame the detection card dropped on the first conveyor belt 9.

[0061] The transmission principle of both the first conveyor belt 9 and the second conveyor belt 10 is conventional roller drive. The speed of both conveyors is adjustable. The first conveyor belt 9 transports the first test card (framed and positioned by the first positioning frame 5) to the designated position. It then pauses, waiting for the sampler 1 to aspirate the incubated mixture in the sample tube 6 and drip it into the sample well of the test card. At this point, the card storage hopper 31 releases the second test card (to be tested). The positioning claws grasp the second test card and move it to the second positioning frame 5, framing and positioning it. After completing these operations, the first conveyor belt 9 moves again to operate the second test card.

[0062] The second conveyor belt 10 moves in the same manner as the first conveyor belt 9, guiding the test cards, after sample addition, within the heating chamber 4. This extends the incubation time of the test cards (compared to continuous movement), thereby improving test accuracy. Test cards removed from the heating chamber 4 can be manually moved into the detector 11. The head and tail of the second conveyor belt 10 are located outside the heating chamber 4, facilitating the receiving and removal of test cards. The second conveyor belt can be positioned directly below or diagonally below the first conveyor belt.

[0063] The detector 11 uses a laser light source to emit light of a specific wavelength to illuminate the test line and quality control line areas of the test card, and then detects the intensity of the reflected light through a highly sensitive photoelectric detection component. The depth of the color of the test line and quality control line is determined based on the intensity of the reflected light, thereby making a qualitative or quantitative judgment on the content of the target substance in the dairy product.

[0064] The test card released from the card storage bin 31 has its length substantially parallel to the length of the first conveyor belt 9. Any slight tilt is corrected by the positioning claws. Specifically, the test card falls onto the first conveyor belt 9, which pauses. The pre-set positioning frame 5 is in the front (i.e., in the forward direction of the conveyor belt 9). The two telescopic rods 17 extend, pushing the two positioning halves 18 toward each other, thereby framing the test card between the two positioning halves 18 (the opening of the square formed by the two positioning halves 18 now faces the opening of the forward positioning frame 5). Simultaneously, the length of the test card is corrected to be parallel to the length of the first conveyor belt 9. The positioning claws move along the slide rails 16 in the direction of the conveyor belt 9, pushing the test card into the forward positioning frame 5. The positioning claws now form a complete square with the positioning frame 5. Then, the two telescopic rods 17 retract, causing the two positioning halves 18 to move away from each other, disengaging from the test card. The positioning claws then return to the position below the card storage bin 31 along the slide rails 16, ready for the next test card to be moved.

[0065] Optionally, the conveying portion 32 is a downwardly inclined slide connecting the tail of the first conveyor belt 9 and the head of the second conveyor belt 10. Vertical guard plates are provided on both sides of the slide to prevent the detection card from falling from the side of the slide.

[0066] Optionally, the test card is a rectangular parallelepiped with a shell, and a sample pad, chromatography test paper, and absorbent filter paper are arranged inside the shell along the length direction of the test card. The chromatography test paper is a nitrocellulose membrane (NC mold), and the chromatography test paper is provided with a quality control line and a test line, and is assembled according to conventional test cards.

[0067] Further optionally, the portion of the upper surface of the housing corresponding to the nitrocellulose membrane is made of transparent plastic material, which is convenient for detection and avoids the influence of changes in ambient humidity on the chromatography reaction;

[0068] A hollow hole is provided in the middle of the upper surface of the shell corresponding to the sample pad. The side walls of the hole protrude outward, and the top of the side wall is heat-sealed with aluminum film. The pipette tip 19 at the bottom of the sampler 1 pierces the aluminum film heat seal and can drip the mixed liquid in the tip into the hole.

[0069] In the traditional test strip format, the sample pad and nitrocellulose membrane are partially exposed, which is easily polluted by the environment and affects the detection accuracy. The present invention adopts a test card format, and the plastic shell wraps the test strip to protect it from environmental influences.

Claims

1. A detection device for gold-labeled immunochromatography of dairy products, characterized in that: It includes a three-dimensional mobile rack, a sample preparation area and a detection area. The sample preparation area includes a sampler, a sealer, a swing rack and an incubation pool. Several swing racks are arranged in a row. A movable sample rack is provided above the swing rack to place several sample tubes. The holes of the sample rack correspond to the holes of the swing rack one by one. The middle and lower parts of the sample tubes are inserted into the corresponding holes on the swing rack. The sampler can penetrate the sealing film of the sample tube to input the dairy sample into the sample tube. The swing rack leads the middle and lower parts of the sample tubes to swing back and forth to evenly mix the gold-labeled antibody with the sample. The sampler and sealer can move along the three-dimensional movable rack; the sealer includes an adsorption plate and a clamping forceps. The adsorption plate can absorb the sealing cover on the incubation pool and buckle it on the top of the sample tube. The clamping forceps can clamp the sample rack into the incubation pool for incubation; The detection area is equipped with a first conveyor belt and a detector. The sampler absorbs the mixed liquid in the incubated sample tube and drops it onto the detection card on the first conveyor belt. The detector is used to detect the chromatography test paper on the detection card to determine the target content in the dairy sample. The swing frame is a frame structure with a plurality of holes on the top. The holes are evenly arranged along the length of the swing frame. The bottom surface of the swing frame is slidably connected to the bottom rail below. The bottom rails correspond to the plurality of swing frames and are arranged in a row. At least one driving device is provided on the side of the swing frame, and the driving device is connected to one end of the swing frame through a rod to drive the swing frame to move back and forth along the bottom track; The sample rack is placed across all rocking racks and bottom rails; The sample tube includes a support part and a container part. The support part is detachably mounted on the top of the container part. The support part is made of hard plastic and is cylindrical with openings at the top and bottom, and is used to support the sample tube on the hole of the sample rack. The container part is made of flexible material and is conical with a larger top and a smaller bottom. It can be inserted into the hole of the swing rack and moved by the swing rack.

2. The detection device for gold-labeled immunochromatography of dairy products according to claim 1, wherein The sampler comprises an air pump and a sampling part which are connected to each other. The air pump is connected to an external air supply device to supply or absorb air to the sampling part. The bottom opening of the sampling part is used to connect a pipette tip.

3. The detection device of dairy gold-labeled immunochromatography according to claim 2, characterized in that, The gold-labeled antibody is freeze-dried and placed in the container. The top of the support is heat-sealed with aluminum film to effectively isolate the gold-labeled antibody from moisture in the environment. The pipette tip pierces the aluminum film to input the dairy sample into the sample tube.

4. The detection device for gold-labeled immunochromatography of dairy products according to claim 1, wherein The sealer includes an electric controller, a controller and a lifting hammer. The electric controller is electrically connected to an external power supply and an adsorption plate. After the adsorption plate is powered, the adsorption plate generates a magnetic attraction force, which can attract the sealing cap to seal the sample tube. The two grippers of the clamping clamp are respectively arranged on both sides of the adsorption plate, and can move closer to or away from each other to clamp or release the two ends of the sample rack; the lifting hammer can move along its own track and perform lifting actions at the same time. The lifting hammer hits the adsorption plate and can buckle the sealing cover on the tube mouth of the sample tube; the controller connects and controls the actions of the lifting hammer and the clamping clamp.

5. The detection device for gold-labeled immunochromatography of dairy products according to claim 4, characterized in that: A driver is provided on the top of the lifting hammer for controlling the lifting hammer to move up and down; the driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

6. The detection device for gold-labeled immunochromatography of dairy products according to claim 4, characterized in that: The sealing cover is a hollow plastic circle, the bottom of the circle can be connected to the top of the sample tube, and the top of the circle is connected to fixed plate one. The part of fixed plate one corresponding to the sealing cover is hollowed out, and fixed plate one and the sealing cover are integrally formed; fixed plate two is provided above fixed plate one, and a plastic film is sandwiched between fixed plate two and fixed plate one so that the plastic film can cover the tube mouth of the sample tube; at least one magnetic block is provided on the upper surface of fixed plate two.

7. The detection device for gold-labeled dairy immunochromatography according to claim 1, characterized in that: The detection area is provided with a card storage bin, a first conveyor belt and a second conveyor belt from top to bottom. The card storage bin is vertical and has several detection cards stacked inside. The first conveyor belt and the second conveyor belt are both horizontal. A conveying part is provided between the first conveyor belt and the second conveyor belt for conveying the detection cards. The outer cover of the second conveyor belt is provided with a heating bin for incubating the detection cards dripped with the mixed liquid to make them react better, and then perform chromatography and color development. A detector is provided at the tail of the second conveyor belt.

8. The detection device for gold-labeled dairy immunochromatography according to claim 7, characterized in that: A plurality of positioning frames are provided on the first conveyor belt, the positioning frames protruding from the upper surface of the first conveyor belt, and the plurality of positioning frames are evenly arranged along the moving direction of the first conveyor belt and arranged in a straight line. The positioning frames are used to clamp the test card, so that the sampler can determine the position of the test card. The positioning frame is a square with three sides, and the opening of the positioning frame faces the opposite direction of the movement of the first conveyor belt.

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