Repairing and soothing composition containing Ectoin

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a repairing and soothing composition containing Ectoine, which is prepared from the following components in percentage by weight: 0.1 to 3 percent of betaine, 0.1 to 2 percent of sophocarpidine, 0.2 to 0.6 percent of 1, 2-hexanediol, 0.2 to 0.6 percent of p-hydroxyacetophenone, 0.2 to 1.2 percent of Ectoine, 0.2 to 1 percent of alfalfa extract, 0.2 to 2 percent of fructus chebulae extract, 0.5 to 3 percent of inositol, 1 to 3 percent of glyceryl polyether-26, 1 to 5 percent of glycerol and the balance of water. The composition has an excellent permeation effect, promotes skin permeation and absorption, and has an excellent repairing and soothing effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a repair and soothing composition containing ectoin. Background Art

[0002] Ectoin, also known as tetrahydromethylpyrimidine carboxylic acid, is a natural active substance extracted from halophilic bacteria. This unique compound was initially discovered in microorganisms that survive in extreme environments, such as deserts, saline-alkali lands, salt lakes, and seawater. These microorganisms can survive under harsh conditions such as high temperature, dryness, strong ultraviolet radiation, and high salinity, and produce ectoin as a natural protective component on the outer layer of cells. Ectoin has extremely strong hydrophilicity and stability, and can protect cells from damage by the external environment.

[0003] In recent years, ectoin has been widely used in cosmetics due to its excellent skin protection effect. However, existing cosmetics containing ectoin often have a single efficacy and cannot meet consumers' diverse needs for skin repair and soothing.

[0004] In view of this, the present application is proposed. Summary of the Invention

[0005] The present invention provides a repair and soothing composition containing ectoin, which has excellent penetration effect, promotes skin penetration and absorption, and has excellent repair and soothing effects.

[0006] The present invention solves its technical problems by adopting the following technical solutions: A repair and soothing composition containing ectoin, characterized in that it comprises the following components in weight percentages: 0.1 - 3% betaine, 0.1 - 2% matrine, 0.2 - 0.6% 1,2 - hexanediol, 0.2 - 0.6% p - hydroxyacetophenone, 0.2 - 1.2% ectoin, 0.2 - 1% alfalfa extract, 0.2 - 2% terminalia chebula extract, 0.5 - 3% inositol, 1 - 3% glycerol polyether - 26, 1 - 5% glycerol, and the balance being water.

[0007] Ectoin (tetrahydromethylpyrimidine carboxylic acid) has good hydrophilicity, can organize and combine the surrounding water molecules and amino acid derivatives, can maintain osmotic pressure balance, reduce excessive evaporation of internal cell water, can protect epidermal immune cells from damage, and at the same time ectoin can stabilize the structure of cell membranes, reduce damage to cell membranes by external factors (such as temperature, free radicals, pH, ultraviolet radiation, and surfactants, etc.), thereby protecting cells from harm. At the same time, ectoin can repair the skin barrier and has strong water replenishing and water - locking capabilities.

[0008] Alfalfa extract can scavenge free radicals, prevent lipid peroxidation, protect antioxidant enzymes, and play an excellent antioxidant role. Alfalfa extract can upregulate the PPARα receptor, increase ceramide synthesis, soothe inflamed skin, and relieve discomfort symptoms such as skin redness and pain.

[0009] Terminalia chebula extract can stabilize cells, strengthen barrier repair, reduce collagen loss, and stimulate the secretion of collagen, thereby delaying skin aging and repairing damaged skin.

[0010] The present invention creatively combines ectoine, alfalfa extract, and Terminalia chebula extract in a specific ratio, and is combined with betaine, matrine, 1,2 - hexanediol, p - hydroxyacetophenone, ectoine, alfalfa extract, Terminalia chebula extract, inositol, glycerol polyether - 26, and glycerol, which can effectively improve the penetration effect of ectoine, alfalfa extract, and Terminalia chebula extract, promote skin penetration and absorption. Ectoine penetrates deep into the skin, combines with water molecules on the outer layer of cells, repairs the skin barrier, and protects cell membranes from damage by external factors; alfalfa extract can increase ceramide synthesis and soothe inflamed skin; Terminalia chebula extract can stabilize cells and strengthen barrier repair. The combined action of the three can significantly repair the skin barrier, relieve discomfort symptoms such as skin redness and pain, and promote the repair and recovery of the skin to a healthy state.

[0011] Among them, the eutectic solvent formed by the combination of betaine, matrine, and glycerol in the present invention through hydrogen bond acceptors and hydrogen bond donors wraps hydroxyprogesterone caproate and ectoine through hydrogen bonds and delivers them deep into the skin. The penetration - promoting system composed of betaine, matrine, and glycerol has good permeability, can promote the active ingredients to reach the effective part of the skin, and fully exert their efficacy.

[0012] As a preferred embodiment of the present invention, it includes the following components in weight percentages: 0.1 - 3% betaine, 0.1 - 2% ectoine, 0.1 - 2% matrine, 0.2 - 0.6% 1,2 - hexanediol, 0.2 - 0.6% p - hydroxyacetophenone, 0.4 - 0.8% ectoine, 0.5 - 0.8% alfalfa extract, 0.8 - 1.5% Terminalia chebula extract, 0.5 - 3% inositol, 1 - 3% glycerol polyether - 26, 1 - 5% glycerol, and the balance is water.

[0013] As a preferred embodiment of the present invention, the preparation method of the Terminalia chebula extract includes the following steps: (1) Crush Terminalia chebula to 50 - 400 meshes to obtain Terminalia chebula powder; (2) Put the obtained Terminalia chebula powder into an extraction kettle for supercritical extraction to obtain an extraction solution; (3) Adjust the pH of the extract to 6.2 - 6.4, and pass it through the macroporous adsorption resin at a flow rate of 0.5 - 1.5 BV / h, and elute at a speed of 0.5 - 1.5 BV / h. First, wash with water for 0.5 - 1.5 BV, then wash with 70 - 80 wt% ethanol solution for 1 - 2 BV, collect the ethanol solution eluate, and dry to obtain the Terminalia chebula extract.

[0014] As a preferred embodiment of the present invention, the supercritical extraction conditions are as follows: using CO2 as the extraction agent, the CO2 flow rate is 50 - 80 mL / min, using 70 - 80 wt% ethanol solution as the entrainer, the extraction temperature is 39 - 42 °C, the extraction pressure is 22 - 26 Mpa, and the extraction time is 40 - 70 min.

[0015] As a preferred embodiment of the present invention, the supercritical extraction conditions are as follows: using CO2 as the extraction agent, the CO2 flow rate is 60 mL / min, using 75 wt% ethanol solution as the entrainer, the extraction temperature is 40 °C, the extraction pressure is 25 Mpa, and the extraction time is 60 min.

[0016] As a preferred embodiment of the present invention, the macroporous adsorption resin is AB-8 macroporous adsorption resin.

[0017] As a preferred embodiment of the present invention, the preparation method of the Medicago sativa extract includes the following steps: (1) Crush Medicago sativa to 50 - 400 mesh to obtain Medicago sativa powder; (2) Add the obtained Medicago sativa powder, cellulase, pectinase, and papain to water and carry out enzymatic hydrolysis to obtain an enzymatic hydrolysate; (3) First filter the enzymatic hydrolysate through an ultrafiltration membrane with a molecular weight cut-off of 1000 Da, then filter it through an ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and dry to obtain the Medicago sativa extract.

[0018] As a preferred embodiment of the present invention, the mass ratio of the Medicago sativa powder, cellulase, pectinase, papain, and water is 1:(0.005 - 0.02):(0.005 - 0.02):(0.005 - 0.02):(2 - 20).

[0019] As a preferred embodiment of the present invention, the mass ratio of the Medicago sativa powder, cellulase, pectinase, papain, and water is 1:0.015:0.01:0.01:8.

[0020] As a preferred embodiment of the present invention, the temperature of the enzymatic hydrolysis is 48 - 52 °C, and the time of the enzymatic hydrolysis is 2 - 6 h.

[0021] Advantages of the present invention: The present invention combines ectoine, alfalfa extract, and terminalia chebula extract in a specific ratio, and is combined with betaine, matrine, 1,2 - hexanediol, p - hydroxyacetophenone, ectoine, alfalfa extract, terminalia chebula extract, inositol, glycerol polyether - 26, and glycerol, which can effectively improve the penetration effect of ectoine, alfalfa extract, and terminalia chebula extract. Ectoine penetrates deep into the skin, combines with water molecules in the outer layer of cells, repairs the skin barrier, and protects cell membranes from damage by external factors; alfalfa extract can increase ceramide synthesis and soothe inflamed skin; terminalia chebula extract can stabilize cells and strengthen barrier repair. The combined action of the three can significantly repair the skin barrier, relieve discomfort symptoms such as skin redness and pain, and promote the repair and recovery of the skin to a healthy state. Detailed implementation mode

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0023] In the present invention, among the technically characterized descriptions in an open - ended manner, it includes a closed - ended technical solution composed of the listed features, and also includes an open - ended technical solution containing the listed features.

[0024] In the present invention, regarding numerical ranges, unless otherwise specified, the above - mentioned numerical ranges are considered continuous, including the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub - ranges subsumed therein.

[0025] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0026] For the reagents or instruments used in the present invention, if not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase. The raw materials used in each comparative ratio and the raw materials used in the parallel experiments of each example are the same commercially available products unless otherwise specified.

[0027] The centella asiatica extract and aloe barbadensis miller leaf extract in the comparative examples of the present invention are both sourced from Shanghai Boshuo Industry Co., Ltd.

[0028] Example 1 A repair and soothing composition containing ectoine, comprising the following components in percentage by weight: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.8% ectoine, 0.8% alfalfa extract, 0.8% Terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0029] The preparation method of the Terminalia chebula extract comprises the following steps: (1) Crush Terminalia chebula to 100 meshes to obtain Terminalia chebula powder; (2) Put the obtained Terminalia chebula powder into an extraction kettle for supercritical extraction to obtain an extraction solution; the supercritical extraction conditions are: using CO2 as the extractant, with a CO2 flow rate of 60 mL / min, using a 75 wt% ethanol solution as the entrainer, an extraction temperature of 40 °C, an extraction pressure of 25 Mpa, and an extraction time of 60 min.

[0030] (3) Adjust the pH of the extraction solution to 6.3, and pass it through AB - 8 macroporous adsorption resin at a flow rate of 1 BV / h, and elute at a speed of 1 BV / h. First, wash with water for 1 BV, then wash with a 75 wt% ethanol solution for 1.5 BV, collect the ethanol solution eluate, and dry to obtain the Terminalia chebula extract.

[0031] The preparation method of the alfalfa extract comprises the following steps: (1) Crush alfalfa to 10 meshes to obtain alfalfa powder; (2) Add the obtained alfalfa powder, cellulase, pectinase, and papain to water, and enzymatically hydrolyze at 50 °C for 4 h to obtain an enzymolysis solution; the mass ratio of alfalfa powder, cellulase, pectinase, papain, and water is 1:0.015:0.01:0.01:8.

[0032] (3) First filter the enzymolysis solution through an ultrafiltration membrane with a molecular weight cut - off of 1000 Da, then filter it through an ultrafiltration membrane with a molecular weight cut - off of 3000 Da, and dry to obtain the alfalfa extract.

[0033] The preparation method of the repair and soothing composition containing ectoine comprises the following steps: Mix betaine, matrine, 1,2 - hexanediol, p - hydroxyacetophenone, ectoine, alfalfa extract, Terminalia chebula extract, inositol, glycerol polyether - 26, glycerol, and water evenly to obtain the repair and soothing composition containing ectoine.

[0034] Example 2 A repair and soothing composition containing ectoine, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.4% ectoine, 0.5% alfalfa extract, 1.5% terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0035] The preparation method of the terminalia chebula extract comprises the following steps: (1) Crush terminalia chebula to 100 meshes to obtain terminalia chebula powder; (2) Put the terminalia chebula powder into an extraction kettle for supercritical extraction to obtain an extract; the supercritical extraction conditions are: using CO2 as the extractant, with a CO2 flow rate of 60 mL / min, using a 75 wt% ethanol solution as the entrainer, an extraction temperature of 40 °C, an extraction pressure of 25 Mpa, and an extraction time of 60 min.

[0036] (3) Adjust the pH of the extract to 6.3, pass it through AB - 8 macroporous adsorption resin at a flow rate of 1 BV / h, and elute at a speed of 1 BV / h. First, wash with water for 1 BV, then wash with a 75 wt% ethanol solution for 1.5 BV, collect the ethanol solution eluate, and dry to obtain the terminalia chebula extract.

[0037] The preparation method of the alfalfa extract comprises the following steps: (1) Crush alfalfa to 10 meshes to obtain alfalfa powder; (2) Add the alfalfa powder, cellulase, pectinase, and papain to water, and enzymolyze at 50 °C for 4 h to obtain an enzymolyzed solution; the mass ratio of the alfalfa powder, cellulase, pectinase, papain, and water is 1:0.015:0.01:0.01:8.

[0038] (3) First filter the enzymolyzed solution through an ultrafiltration membrane with a molecular weight cut - off of 1000 Da, then filter it through an ultrafiltration membrane with a molecular weight cut - off of 3000 Da, and dry to obtain the alfalfa extract.

[0039] The preparation method of the repair and soothing composition containing ectoine comprises the following steps: Mix betaine, matrine, 1,2 - hexanediol, p - hydroxyacetophenone, ectoine, alfalfa extract, terminalia chebula extract, inositol, glycerol polyether - 26, glycerol, and water evenly to obtain the repair and soothing composition containing ectoine.

[0040] Example 3 A repair and soothing composition containing ectoine, comprising the following components in percentage by weight: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.2% ectoine, 0.2% alfalfa extract, 2% Terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance water.

[0041] The preparation method of the Terminalia chebula extract comprises the following steps: (1) Crush Terminalia chebula to 100 meshes to obtain Terminalia chebula powder; (2) Put the obtained Terminalia chebula powder into an extraction kettle for supercritical extraction to obtain an extract; the supercritical extraction conditions are: using CO2 as the extraction agent, the CO2 flow rate is 60 mL / min, using 75 wt% ethanol solution as the entrainer, the extraction temperature is 40 °C, the extraction pressure is 25 Mpa, and the extraction time is 60 min.

[0042] (3) Adjust the pH of the extract to 6.3, pass it through AB - 8 macroporous adsorption resin at a flow rate of 1 BV / h, and elute at a speed of 1 BV / h. First, wash with water for 1 BV, then wash with 75 wt% ethanol solution for 1.5 BV, collect the ethanol solution eluate, and dry to obtain the Terminalia chebula extract.

[0043] The preparation method of the alfalfa extract comprises the following steps: (1) Crush alfalfa to 10 meshes to obtain alfalfa powder; (2) Add the obtained alfalfa powder, cellulase, pectinase, and papain to water, and enzymatically hydrolyze at 50 °C for 4 h to obtain an enzymolysis solution; the mass ratio of alfalfa powder, cellulase, pectinase, papain, and water is 1:0.015:0.01:0.01:8.

[0044] (3) First filter the enzymolysis solution through an ultrafiltration membrane with a molecular weight cut - off of 1000 Da, then filter it through an ultrafiltration membrane with a molecular weight cut - off of 3000 Da, and dry to obtain the alfalfa extract.

[0045] The preparation method of the repair and soothing composition containing ectoine comprises the following steps: Mix betaine, matrine, 1,2 - hexanediol, p - hydroxyacetophenone, ectoine, alfalfa extract, Terminalia chebula extract, inositol, glycerol polyether - 26, glycerol, and water evenly to obtain the repair and soothing composition containing ectoine.

[0046] Example 4 A repairing and soothing composition containing ectoine, comprising the following components in weight percentage: 0.5% betaine, 0.2% matrine, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.1.2% ectoine, 1% alfalfa extract, 0.2% terminalia chebula extract, 1% inositol, 2% glyceryl polyether-26, 4% glycerin, and the balance water.

[0047] The preparation method of the terminalia chebula extract comprises the following steps: (1) crushing the terminalia chebula into 100 meshes to obtain terminalia chebula powder; (2) Putting the terminalia chebula powder into an extraction kettle and performing supercritical extraction to obtain an extract; the supercritical extraction conditions are: using CO2 as an extractant, a CO2 flow rate of 60 mL / min, using 75 wt% ethanol solution as an entrainer, an extraction temperature of 40°C, an extraction pressure of 25 MPa, and an extraction time of 60 min.

[0048] (3) The extract was adjusted to pH 6.3, passed through AB-8 macroporous adsorption resin at a flow rate of 1 BV / h, and eluted at a rate of 1 BV / h, first washing 1 BV with water, then washing 1.5 BV with 75 wt% ethanol solution, collecting the ethanol solution eluate, and drying to obtain the Terminalia chebula extract.

[0049] The preparation method of the alfalfa extract comprises the following steps: (1) Grinding alfalfa into 10 meshes to obtain alfalfa powder; (2) Adding alfalfa powder, cellulase, pectinase and papain into water, performing enzymolysis at 50° C. for 4 h, and obtaining an enzymolysis solution; the mass ratio of the alfalfa powder, cellulase, pectinase, papain and water is 1:0.015:0.01:0.01:8.

[0050] (3) The enzymatic hydrolyzate is first filtered through an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and then filtered through an ultrafiltration membrane with a molecular weight cutoff of 3000Da, and dried to obtain an alfalfa extract.

[0051] The preparation method of the repairing and soothing composition containing ectoine comprises the following steps: betaine, matrine, 1,2-hexanediol, p-hydroxyacetophenone, ectoine, alfalfa extract, terminalia chebula extract, inositol, glycerol polyether-26, glycerol and water are uniformly mixed to obtain the repairing and soothing composition containing ectoine.

[0052] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain ectoine, and all other aspects are the same.

[0053] A repair and soothing composition containing ectoin, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.8% alfalfa extract, 0.8% terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0054] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain alfalfa extract, and the others are the same.

[0055] A repair and soothing composition containing ectoin, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.8% ectoin, 0.8% terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0056] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain terminalia chebula extract, and the others are the same.

[0057] A repair and soothing composition containing ectoin, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.8% ectoin, 0.8% alfalfa extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0058] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain ectoin (the total amount of ectoin, alfalfa extract, and terminalia chebula extract remains unchanged), and the others are the same.

[0059] A repair and soothing composition containing ectoin, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 1.2% alfalfa extract, 1.2% terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0060] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain alfalfa extract (the total amount of ectoin, alfalfa extract, and terminalia chebula extract remains unchanged), and the others are the same.

[0061] A repair and soothing composition containing ectoine, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.1.2% ectoine, 1.2% Terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0062] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain Terminalia chebula extract (the total amount of ectoine, Medicago sativa extract, and Terminalia chebula extract remains unchanged), and the others are the same.

[0063] A repair and soothing composition containing ectoine, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.1.2% ectoine, 1.2% Medicago sativa extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0064] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 uses an equal amount of Centella asiatica extract to replace Medicago sativa extract, and the others are the same.

[0065] A repair and soothing composition containing ectoine, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.0.8% ectoine, 0.8% Centella asiatica extract, 0.8% Terminalia chebula extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0066] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 uses an equal amount of Aloe barbadensis Miller leaf extract to replace Terminalia chebula extract, and the others are the same.

[0067] A repair and soothing composition containing ectoine, comprising components in the following weight percentages: 0.5% betaine, 0.2% matrine, 0.5% 1,2 - hexanediol, 0.5% p - hydroxyacetophenone, 0.0.8% ectoine, 0.8% Medicago sativa extract, 0.8% Aloe barbadensis Miller leaf extract, 1% inositol, 2% glycerol polyether - 26, 4% glycerol, and the balance being water.

[0068] Test Example 1. The hyaluronidase activity inhibition rate was tested using T / JSRH 003 - 2023 "Test Method for Antihistamine Activity of Cosmetic Raw Materials - Hyaluronidase Inhibition Method". Each experiment was repeated three times, and the average value was taken as the final result.

[0069] 2. HaCaT (human keratinocytes) were cultured in RPMI-1640 medium containing 120 U / ml penicillin, 120 μg / ml streptomycin, and 12% fetal bovine serum at 37°C and 5% CO2 with 98% humidity. Logarithmic growth phase cells were selected and seeded into 96-well culture plates. After the cells were completely adherent after 24 h of culture, the medium was replaced with RPMI 1640 medium containing the compositions of the examples and comparative examples (final concentration 0.12 mg / mL). After incubation for 24 h, UVB irradiation was performed at a dose of 18 mJ / cm 2 The cells were irradiated at a distance of 12 cm. After irradiation, the cell culture supernatant was collected, and the concentration of IL-1α in the supernatant was detected using a human ELISA kit. Each experiment was repeated three times, and the average value was taken as the final result.

[0070] Table 1

[0071] As can be seen from Table 1, the composition of the present invention has excellent repair and soothing effects.

[0072] Comparing Example 1 with Comparative Examples 1-8, it can be seen that ectoine, alfalfa extract, and terminalia chebula extract of the present invention have a significant synergistic effect in terms of repair and soothing. Through the synergistic effect of the three, the repair and soothing effect of the composition is significantly improved. If any one of them is missing or replaced with other ingredients, the repair and soothing effect will be significantly reduced.

[0073] Comparing Examples 1-2 with Examples 3-4, by controlling the amounts of each raw material as follows: 0.1-3% betaine, 0.1-2% ectoine, 0.1-2% matrine, 0.2-0.6% 1,2-hexanediol, 0.2-0.6% p-hydroxyacetophenone, 0.4-0.8% ectoine, 0.5-0.8% alfalfa extract, 0.8-1.5% terminalia chebula extract, 0.5-3% inositol, 1-3% glycerol polyether-26, 1-5% glycerol, and the balance being water, the repair and soothing effect is further improved.

[0074] Finally, it should be noted that the above examples are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A repairing and soothing composition containing ectoine, characterized in that: The invention comprises the following components in percentage by weight: 0.1-3% betaine, 0.1-2% matrine, 0.2-0.6% 1,2-hexanediol, 0.2-0.6% p-hydroxyacetophenone, 0.2-1.2% ectoine, 0.2-1% alfalfa extract, 0.2-2% terminalia chebula extract, 0.5-3% inositol, 1-3% glycerol polyether-26, 1-5% glycerol and the balance water.

2. The repairing and soothing composition containing ectoine according to claim 1, characterized in that: The invention comprises the following components in percentage by weight: 0.1-3% betaine, 0.1-2% tetrahydromethylpyrimidine carboxylic acid, 0.1-2% matrine, 0.2-0.6% 1,2-hexanediol, 0.2-0.6% p-hydroxyacetophenone, 0.4-0.8% ectoine, 0.5-0.8% alfalfa extract, 0.8-1.5% terminalia chebula extract, 0.5-3% inositol, 1-3% glycerol polyether-26, 1-5% glycerol and the balance water.

3. The repairing and soothing composition containing ectoine according to claim 1, characterized in that: The preparation method of the terminalia chebula extract comprises the following steps: (1) crushing the terminalia chebula into 50-400 meshes to obtain terminalia chebula powder; (2) placing the terminalia chebula powder into an extraction kettle and performing supercritical extraction to obtain an extract; (3) The pH value of the extract is adjusted to 6.2-6.4, and the extract is passed through the macroporous adsorption resin at a flow rate of 0.5-1.5 BV / h. The extract is eluted at a rate of 0.5-1.5 BV / h, firstly washed with water for 0.5-1.5 BV, and then washed with 70-80 wt% ethanol solution for 1-2 BV. The ethanol solution eluate is collected and dried to obtain the Terminalia chebula extract.

4. The repairing and soothing composition containing ectoine according to claim 3, characterized in that: The supercritical extraction conditions are: CO2 as the extractant, the CO2 flow rate is 50~80mL / min, 70~80wt% ethanol solution is used as the entrainer, the extraction temperature is 39~42°C, the extraction pressure is 22~26Mpa, and the extraction time is 40~70min.

5. The repairing and soothing composition containing ectoine according to claim 3, characterized in that: The supercritical extraction conditions are: CO2 as the extractant, the CO2 flow rate is 60 mL / min, 75 wt% ethanol solution is used as the entrainer, the extraction temperature is 40°C, the extraction pressure is 25 MPa, and the extraction time is 60 min.

6. The repairing and soothing composition containing ectoine according to claim 3, characterized in that: The macroporous adsorption resin is AB-8 macroporous adsorption resin.

7. The repairing and soothing composition containing ectoine according to claim 1, characterized in that: The preparation method of the alfalfa extract comprises the following steps: (1) Grinding alfalfa to 50-400 mesh to obtain alfalfa powder; (2) adding alfalfa powder, cellulase, pectinase and papain into water for enzymolysis to obtain an enzymolysis solution; (3) The enzymatic hydrolyzate is first filtered through an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and then filtered through an ultrafiltration membrane with a molecular weight cutoff of 3000Da, and dried to obtain an alfalfa extract.

8. The repairing and soothing composition containing ectoine according to claim 7, characterized in that: The mass ratio of the alfalfa powder, cellulase, pectinase, papain and water is 1: (0.005-0.02): (0.005-0.02): (0.005-0.02): (2-20).

9. The repairing and soothing composition containing ectoine according to claim 7, characterized in that: The mass ratio of the alfalfa powder, cellulase, pectinase, papain and water is 1:0.015:0.01:0.01:

8.

10. The repairing and soothing composition containing ectoine according to claim 7, characterized in that: The enzymolysis temperature is 48-52° C., and the enzymolysis time is 2-6 hours.

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