Herba epimedii extract, preparation method thereof and application of herba epimedii extract in preparation of products with anti-aging effect

Abstract

The invention discloses a herba epimedii extract, a preparation method of the herba epimedii extract and application of the herba epimedii extract in preparation of a product with an anti-aging effect. The preparation method comprises the following steps: (1) taking herba epimedii, extracting the herba epimedii with ethanol, separating an extracting solution after the extraction is finished, and concentrating and drying the extracting solution to obtain a herba epimedii ethanol extract; (2) suspending the ethanol extract in water, and extracting with ethyl acetate; taking the ethyl acetate extract liquor, concentrating and drying to obtain a herba epimedii ethyl acetate extract; (3) loading the herba epimedii ethyl acetate extract on a macroporous resin column, and eluting with a solvent to obtain a herba epimedii macroporous resin elution part; and taking the macroporous resin elution part of the herba epimedii to obtain the herba epimedii extract. Research shows that the herba epimedii extract prepared by the method disclosed by the invention has a relatively good anti-aging effect; therefore, when being used as an active ingredient for preparing skin care products or medicines with anti-aging effects, the compound has important application value.

Description

Technical Field

[0001] The present invention relates to the technical field of natural medicine chemistry, and particularly relates to an epimedium extract, a preparation method thereof, and an application thereof in the preparation of a product with anti-aging effect. Background Art

[0002] Epimedium, also known as Xianlingpi and Gangqian; it has the effects of tonifying kidney yang, strengthening tendons and bones, and dispelling wind and dampness, and is mainly used for impotence and spermatorrhea, flaccidity of tendons and bones, rheumatic arthralgia, numbness and contracture caused by kidney yang deficiency, and is a commonly used traditional Chinese medicine in clinical practice. With the development of modern science and technology, various pharmacological effects of epimedium have been deeply studied, especially showing significant effects in aspects such as antioxidant, anti-inflammatory, and promoting cell proliferation. These characteristics make epimedium an ideal candidate material for developing anti-aging products.

[0003] However, the epimedium extract obtained by the existing technology only using a simple extraction method has poor anti-aging effect, and often needs to be compounded with other extracts to obtain a better anti-aging effect. Therefore, providing a preparation method of an epimedium extract with good anti-aging effect has important application value. Summary of the Invention

[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides the following technical solutions: The present invention first provides a preparation method of an epimedium extract, which comprises the following steps: (1) Take epimedium, extract with ethanol, separate the extract after the extraction is completed, and concentrate and dry the extract to obtain an epimedium ethanol extract; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, concentrate and dry to obtain an epimedium ethyl acetate extract; (3) Load the epimedium ethyl acetate extract onto a macroporous resin column, elute with a solvent to obtain an elution fraction of epimedium on the macroporous resin; take the elution fraction of epimedium on the macroporous resin to obtain the epimedium extract.

[0005] The present invention provides a brand-new preparation method of an epimedium extract. Research shows that the epimedium extract prepared by the method of the present invention has good anti-aging effect; its anti-aging effect is much higher than that of the epimedium ethyl acetate extract obtained only by extracting epimedium with ethanol and then extracting with ethyl acetate.

[0006] Preferably, the ethanol in step (1) specifically refers to an ethanol aqueous solution with a volume fraction of 30% - 70%.

[0007] Preferably, the ethanol described in step (1) specifically refers to an ethanol aqueous solution with a volume fraction of 50%.

[0008] Preferably, the dosage ratio of epimedium to ethanol in step (1) is 1 kg: 10 - 20 L.

[0009] Most preferably, the dosage ratio of epimedium to ethanol in step (1) is 1 kg: 15 L.

[0010] Preferably, the extraction in step (1) is heating reflux extraction; the temperature of the heating reflux extraction is 70 - 80 °C, and the time of the heating reflux extraction is 1 - 3 h.

[0011] Most preferably, the extraction in step (1) is heating reflux extraction; the temperature of the heating reflux extraction is 75 °C, and the time of the heating reflux extraction is 1 - 3 h.

[0012] Preferably, the specific conditions for elution with a solvent in step (3) are: Load the ethyl acetate extract of epimedium onto a macroporous resin column, first elute with an ethanol aqueous solution with a volume fraction of 32% - 34% to remove impurities; then elute with an ethanol aqueous solution with a volume fraction of 46% - 48%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 46% - 48%, concentrate and dry it to obtain the macroporous resin elution part of epimedium; take the macroporous resin elution part of epimedium to obtain the epimedium extract.

[0013] Preferably, the specific conditions for elution with a solvent in step (3) are: Load the ethyl acetate extract of epimedium onto a macroporous resin column, first elute with an ethanol aqueous solution with a volume fraction of 33% to remove impurities; then elute with an ethanol aqueous solution with a volume fraction of 47%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 47%, concentrate and dry it to obtain the macroporous resin elution part of epimedium; take the macroporous resin elution part of epimedium to obtain the epimedium extract.

[0014] The inventor found in a large number of studies that the elution conditions of the macroporous resin column in the present invention are very crucial; only the macroporous resin elution part of epimedium prepared by further eluting the ethyl acetate extract of epimedium under the elution conditions of the macroporous resin column in the present invention has an anti - aging effect that is significantly higher than that of the ethyl acetate extract of epimedium; however, the macroporous resin elution part of epimedium prepared under other macroporous resin column elution conditions does not have an anti - aging effect that is significantly higher than that of the ethyl acetate extract of epimedium.

[0015] Preferably, the method for preparing the epimedium extract further comprises the following steps: subjecting the macroporous resin elution fraction of epimedium to a silica gel column, first eluting with mixed solvent A to remove impurities; then eluting with mixed solvent B, collecting the eluate eluted by mixed solvent B, concentrating and drying to obtain the silica gel elution fraction of epimedium; taking the silica gel elution fraction of epimedium to obtain the epimedium extract as described above.

[0016] Preferably, the mixed solvent A is a mixed solvent composed of chloroform and acetone with a volume ratio of 75 - 77:25 - 23; the mixed solvent B is a mixed solvent composed of chloroform and acetone with a volume ratio of 55 - 60:45 - 40.

[0017] Most preferably, the mixed solvent A is a mixed solvent composed of chloroform and acetone with a volume ratio of 76:24; the mixed solvent B is a mixed solvent composed of chloroform and acetone with a volume ratio of 57:43.

[0018] The inventors further found in their research that the epimedium extract prepared by further subjecting the macroporous resin elution fraction of epimedium to elution through the silica gel column of the present invention has very excellent anti - aging effects, and its anti - aging effect is significantly higher than that of the macroporous resin elution fraction of epimedium.

[0019] The inventors also found in a large number of studies that the elution conditions of the silica gel column of the present invention are very crucial; only when the macroporous resin elution fraction of epimedium is further prepared into an epimedium extract under the elution conditions of the silica gel column of the present invention can its anti - aging effect be significantly higher than that of the macroporous resin elution fraction of epimedium; however, for the epimedium extract prepared under other silica gel column elution conditions, its anti - aging effect cannot be significantly higher than that of the macroporous resin elution fraction of epimedium.

[0020] The present invention also provides an epimedium extract prepared by the above - mentioned preparation method.

[0021] The present invention also provides an application of the above - mentioned epimedium extract in the preparation of a product with anti - aging effects.

[0022] Preferably, the product is a skin care product or a drug.

[0023] Beneficial effects: The present invention provides a brand - new method for preparing an epimedium extract. Research shows that the epimedium extract prepared by the method of the present invention has good anti - aging effects; its anti - aging effect is significantly higher than that of the ethyl acetate extract of epimedium obtained only through ethanol extraction and ethyl acetate extraction steps of epimedium. Therefore, using the epimedium extract of the present invention as an active ingredient for preparing skin care products or drugs with anti - aging effects has important application value. Detailed implementation mode

[0024] The present invention will be further explained below in conjunction with specific embodiments, but the embodiments do not limit the present invention in any form.

[0025] Example 1 Preparation of Epimedium Extract (1) Take the traditional Chinese medicine Epimedium, heat it with ethanol to 75 °C and reflux for 1 h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the Epimedium ethanol extract; the ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%; the dosage ratio of Epimedium to ethanol is 1 kg:15 L; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, Concentrate and dry to obtain the Epimedium ethyl acetate extract; among them, the dosage ratio of the ethanol extract to water and ethyl acetate is 1 kg:5 L:5 L; (3) Load 30 times the weight of the Epimedium ethyl acetate extract of D101 macroporous resin into a glass column to obtain a macroporous resin column; load the Epimedium ethyl acetate extract onto the macroporous resin column, first elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 33% to remove impurities; then elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 47%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 47%, concentrate and dry to obtain the Epimedium macroporous resin elution fraction; take the Epimedium macroporous resin elution fraction to obtain the Epimedium extract.

[0026] Example 2 Preparation of Epimedium Extract (1) Take the traditional Chinese medicine Epimedium, heat it with ethanol to 75 °C and reflux for 1 h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain the Epimedium ethanol extract; the ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%; the dosage ratio of Epimedium to ethanol is 1 kg:15 L; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, Concentrate and dry to obtain the Epimedium ethyl acetate extract; among them, the dosage ratio of the ethanol extract to water and ethyl acetate is 1 kg:5 L:5 L; (3) Load 30 times the weight of the Epimedium ethyl acetate extract of D101 macroporous resin into a glass column to obtain a macroporous resin column; load the Epimedium ethyl acetate extract onto the macroporous resin column, first elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 33% to remove impurities; then elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 47%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 47%, concentrate and dry to obtain the Epimedium macroporous resin elution fraction; (4) Weigh 30 times the weight of the elution fraction of Epimedium grandiflorum Morr. on macroporous resin and load 200 - 300 mesh silica gel into a glass column to obtain a silica gel column. Load the elution fraction of Epimedium grandiflorum Morr. on macroporous resin onto the silica gel column. First, elute with 3 column volumes of mixed solvent A to remove impurities. Then, elute with 6 column volumes of mixed solvent B, collect the eluate eluted by mixed solvent B, concentrate and dry it to obtain the elution fraction of Epimedium grandiflorum Morr. on silica gel. Take the elution fraction of Epimedium grandiflorum Morr. on silica gel to obtain the Epimedium grandiflorum Morr. extract. The mixed solvent A is a mixed solvent composed of chloroform and acetone with a volume ratio of 76:24. The mixed solvent B is a mixed solvent composed of chloroform and acetone with a volume ratio of 57:43.

[0027] Comparative Example 1 Preparation of Epimedium grandiflorum Morr. Extract (1) Take the traditional Chinese medicine Epimedium grandiflorum Morr., heat it with ethanol to 75 °C and reflux for 1 h. After the extraction is completed, separate the extract, concentrate and dry it to obtain the ethanol extract of Epimedium grandiflorum Morr. The ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%. The dosage ratio of Epimedium grandiflorum Morr. to ethanol is 1 kg:15 L. (2) Suspend the ethanol extract in water, and then extract it with ethyl acetate. Take the ethyl acetate extract, concentrate and dry it to obtain the ethyl acetate extract of Epimedium grandiflorum Morr. Among them, the dosage ratio of the ethanol extract, water and ethyl acetate is 1 kg:5 L:5 L. Take the ethyl acetate extract of Epimedium grandiflorum Morr. to obtain the Epimedium grandiflorum Morr. extract.

[0028] Comparative Example 2 Preparation of Epimedium grandiflorum Morr. Extract (1) Take the traditional Chinese medicine Epimedium grandiflorum Morr., heat it with ethanol to 75 °C and reflux for 1 h. After the extraction is completed, separate the extract, concentrate and dry it to obtain the ethanol extract of Epimedium grandiflorum Morr. The ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%. The dosage ratio of Epimedium grandiflorum Morr. to ethanol is 1 kg:15 L. (2) Suspend the ethanol extract in water, and then extract it with ethyl acetate. Take the ethyl acetate extract, concentrate and dry it to obtain the ethyl acetate extract of Epimedium grandiflorum Morr. Among them, the dosage ratio of the ethanol extract, water and ethyl acetate is 1 kg:5 L:5 L. (3) Weigh 30 times the weight of the ethyl acetate extract of Epimedium grandiflorum Morr. and load D101 macroporous resin into a glass column to obtain a macroporous resin column. Load the ethyl acetate extract of Epimedium grandiflorum Morr. onto the macroporous resin column. First, elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 15% to remove impurities. Then, elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 30%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 30%, concentrate and dry it to obtain the elution fraction of Epimedium grandiflorum Morr. on macroporous resin. Take the elution fraction of Epimedium grandiflorum Morr. on macroporous resin to obtain the Epimedium grandiflorum Morr. extract.

[0029] Comparative Example 3 Preparation of Epimedium Extract (1) Take the traditional Chinese medicine Epimedium, heat it with ethanol to 75°C and carry out reflux extraction for 1 h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain Epimedium ethanol extract; the ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%; the dosage ratio of Epimedium to ethanol is 1 kg:15 L; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, concentrate and dry to obtain Epimedium ethyl acetate extract; among them, the dosage ratio of ethanol extract to water and ethyl acetate is 1 kg:5 L:5 L; (3) Load 30 times the weight of Epimedium ethyl acetate extract of D101 macroporous resin into a glass column to obtain a macroporous resin column; load the Epimedium ethyl acetate extract onto the macroporous resin column, first elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 40% to remove impurities; then elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 60%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 60%, concentrate and dry to obtain the macroporous resin elution part of Epimedium; take the macroporous resin elution part of Epimedium to obtain the Epimedium extract.

[0030] Comparative Example 4 Preparation of Epimedium Extract (1) Take the traditional Chinese medicine Epimedium, heat it with ethanol to 75°C and carry out reflux extraction for 1 h. After the extraction is completed, separate the extract, concentrate and dry the extract to obtain Epimedium ethanol extract; the ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%; the dosage ratio of Epimedium to ethanol is 1 kg:15 L; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, concentrate and dry to obtain Epimedium ethyl acetate extract; among them, the dosage ratio of ethanol extract to water and ethyl acetate is 1 kg:5 L:5 L; (3) Load 30 times the weight of Epimedium ethyl acetate extract of D101 macroporous resin into a glass column to obtain a macroporous resin column; load the Epimedium ethyl acetate extract onto the macroporous resin column, first elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 33% to remove impurities; then elute with 5 column volumes of an ethanol aqueous solution with a volume fraction of 47%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 47%, concentrate and dry to obtain the macroporous resin elution part of Epimedium; (4) Weigh 30 times the weight of the macroporous resin elution fraction of Epimedium brevicornum and load 200 - 300 mesh silica gel into a glass column to obtain a silica gel column; load the macroporous resin elution fraction of Epimedium brevicornum onto the silica gel column, first elute with 3 column volumes of mixed solvent A to remove impurities; then elute with 6 column volumes of mixed solvent B, collect the eluate eluted by mixed solvent B, concentrate and dry it to obtain the silica gel elution fraction of Epimedium brevicornum; take the silica gel elution fraction of Epimedium brevicornum to obtain the Epimedium brevicornum extract; the mixed solvent A is a mixed solvent composed of chloroform and acetone with a volume ratio of 90:10; the mixed solvent B is a mixed solvent composed of chloroform and acetone with a volume ratio of 75:25.

[0031] Comparative Example 5 Preparation of Epimedium brevicornum Extract (1) Take the traditional Chinese medicine Epimedium brevicornum, heat it with ethanol at 75 °C for reflux extraction for 1 h, separate the extract after extraction, concentrate and dry the extract to obtain the ethanol extract of Epimedium brevicornum; the ethanol specifically refers to an ethanol aqueous solution with a volume fraction of 50%; the dosage ratio of Epimedium brevicornum to ethanol is 1 kg:15 L; (2) Suspend the ethanol extract in water, and then extract with ethyl acetate; take the ethyl acetate extract, concentrate and dry it to obtain the ethyl acetate extract of Epimedium brevicornum; among them, the dosage ratio of the ethanol extract to water and ethyl acetate is 1 kg:5 L:5 L; (3) Weigh 30 times the weight of the ethyl acetate extract of Epimedium brevicornum and load D101 macroporous resin into a glass column to obtain a macroporous resin column; load the ethyl acetate extract of Epimedium brevicornum onto the macroporous resin column, first elute with 5 column volumes of ethanol aqueous solution with a volume fraction of 33% to remove impurities; then elute with 5 column volumes of ethanol aqueous solution with a volume fraction of 47%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 47%, concentrate and dry it to obtain the macroporous resin elution fraction of Epimedium brevicornum; (4) Weigh 30 times the weight of the macroporous resin elution fraction of Epimedium brevicornum and load 200 - 300 mesh silica gel into a glass column to obtain a silica gel column; load the macroporous resin elution fraction of Epimedium brevicornum onto the silica gel column, first elute with 3 column volumes of mixed solvent A to remove impurities; then elute with 6 column volumes of mixed solvent B, collect the eluate eluted by mixed solvent B, concentrate and dry it to obtain the silica gel elution fraction of Epimedium brevicornum; take the silica gel elution fraction of Epimedium brevicornum to obtain the Epimedium brevicornum extract; the mixed solvent A is a mixed solvent composed of chloroform and acetone with a volume ratio of 60:40; the mixed solvent B is a mixed solvent composed of chloroform and acetone with a volume ratio of 40:60.

[0032] Example 3 Anti - aging Experiment 800 Caenorhabditis elegans at the L4 stage were randomly divided into 8 groups, with 100 in each group, namely experimental groups 1 - 7 and a blank control group. Experimental groups 1 - 7 were respectively cultured on NGM plates containing 0.1 mg / mL of the to - be - tested epimedium extract; the blank control group was cultured on NGM plates without the to - be - tested epimedium extract. After 25 days of culture, the survival rates of Caenorhabditis elegans in each group were calculated; the results are shown in Table 1.

[0033]

[0034] It can be seen from the experimental results in Table 1 that the blank control group was cultured on NGM plates without adding epimedium extract, and its survival time did not exceed 25 days. While experimental group 3 was cultured on NGM plates containing the epimedium extract prepared in Comparative Example 1, and the survival rate of Caenorhabditis elegans after 25 days of culture was 12%, which could effectively extend the lifespan of Caenorhabditis elegans, having a certain anti - aging effect, but the effect was not obvious.

[0035] It can also be seen from the experimental results in Table 1 that the survival rate of Caenorhabditis elegans in experimental group 1 was much higher than that in experimental group 3; this indicates that: the epimedium extract prepared by the method of the present invention has a better anti - aging effect; its anti - aging effect is much higher than that of the ethyl acetate extract of epimedium obtained only by ethanol extraction and ethyl acetate extraction of epimedium. It can also be seen from the experimental results in Table 1 that compared with experimental group 3, the survival rates of Caenorhabditis elegans in experimental groups 4 and 5 increased, but the increase amplitude was much smaller than that in experimental group 1; this indicates that: the elution conditions of the macroporous resin column in the present invention are very crucial; only the macroporous resin elution part of epimedium prepared under the elution conditions of the macroporous resin column of the present invention from the ethyl acetate extract of epimedium can have an anti - aging effect much higher than that of the ethyl acetate extract of epimedium; however, the macroporous resin elution part of epimedium prepared under other macroporous resin column elution conditions does not have an anti - aging effect much higher than that of the ethyl acetate extract of epimedium.

[0036] It can also be seen from the experimental results in Table 1 that the survival rate of Caenorhabditis elegans in Experimental Group 2 reached 81%, which was significantly higher than that in Experimental Group 1; this indicates that the Epimedium extract prepared by further eluting the macroporous resin elution fraction of Epimedium with the above-mentioned silica gel column of the present invention has very excellent anti-aging effects, and its anti-aging effects are significantly higher than those of the macroporous resin elution fraction of Epimedium. It can also be seen from the experimental results in Table 1 that although the survival rates of Caenorhabditis elegans in Experimental Groups 6 and 7 were higher than that in Experimental Group 1, the increase was much smaller than that in Experimental Group 2; this indicates that the elution conditions of the silica gel column of the present invention are very crucial; only the Epimedium extract prepared by further eluting the macroporous resin elution fraction of Epimedium under the elution conditions of the silica gel column of the present invention can have significantly higher anti-aging effects than the macroporous resin elution fraction of Epimedium; however, the anti-aging effects of the Epimedium extract prepared under other silica gel column elution conditions cannot be significantly higher than those of the macroporous resin elution fraction of Epimedium.

Claims

1. A method for preparing an epimedium extract, characterized in that: The following steps are included: (1) extracting epimedium with ethanol, separating the extract after the extraction, concentrating and drying the extract to obtain epimedium ethanol extract; (2) Suspend the ethanol extract in water and then extract with ethyl acetate; take the ethyl acetate extract, After concentration and drying, the ethyl acetate extract of Epimedium was obtained; (3) The ethyl acetate extract of epimedium is loaded onto a macroporous resin column and eluted with a solvent to obtain an epimedium macroporous resin elution fraction; the epimedium macroporous resin elution fraction is obtained to obtain the epimedium extract.

2. The method for preparing the epimedium extract according to claim 1, characterized in that: The ethanol described in step (1) specifically refers to an ethanol aqueous solution with a volume fraction of 30% to 70%.

3. The method for preparing the epimedium extract according to claim 1, characterized in that: The ethanol described in step (1) specifically refers to an ethanol aqueous solution with a volume fraction of 50%.

4. The method for preparing the epimedium extract according to claim 1, characterized in that: In step (1), the ratio of epimedium to ethanol is 1 kg: 10-20 L; Most preferably, the ratio of epimedium to ethanol in step (1) is 1 kg:15 L.

5. The method for preparing the epimedium extract according to claim 1, characterized in that: The extraction in step (1) is a heating reflux extraction; the temperature of the heating reflux extraction is 70-80°C, and the time of the heating reflux extraction is 1-3h; Most preferably, the extraction in step (1) is a heating reflux extraction; the temperature of the heating reflux extraction is 75°C, and the time of the heating reflux extraction is 1 to 3 hours.

6. The method for preparing the epimedium extract according to claim 1, characterized in that: The specific conditions for eluting with a solvent in step (3) are: The ethyl acetate extract of epimedium is placed on a macroporous resin column, and impurities are first eluted with an ethanol aqueous solution with a volume fraction of 32% to 34%; then, it is eluted with an ethanol aqueous solution with a volume fraction of 46% to 48%, and the eluate eluted with the ethanol aqueous solution with a volume fraction of 46% to 48% is collected, and after concentration and drying, an epimedium macroporous resin elution fraction is obtained; and the epimedium macroporous resin elution fraction is taken to obtain the epimedium extract.

7. The method for preparing the epimedium extract according to claim 1, characterized in that: The specific conditions for eluting with a solvent in step (3) are: The ethyl acetate extract of epimedium is placed on a macroporous resin column, and impurities are first eluted with an ethanol aqueous solution with a volume fraction of 33%; then, it is eluted with an ethanol aqueous solution with a volume fraction of 47%, and the eluate eluted with the ethanol aqueous solution with a volume fraction of 47% is collected, and after concentration and drying, an epimedium macroporous resin elution fraction is obtained; and the epimedium macroporous resin elution fraction is taken to obtain the epimedium extract.

8. The epimedium extract prepared by the preparation method according to any one of claims 1 to 7.

9. Use of the epimedium extract according to claim 8 in preparing a product with anti-aging effect.

10. The use according to claim 9, characterized in that: The product is a skin care product or a medicine.

Images