Europium-labeled antibody dilution solution reagent strip preparation device and preparation method thereof

Abstract

The invention relates to the technical field of europium-labeled antibody preparation, in particular to a europium-labeled antibody dilution solution reagent strip preparation device and a preparation method thereof.The europium-labeled antibody dilution solution reagent strip preparation device comprises a supporting frame, a mixing tank is installed on one side of the top of the supporting frame, a reagent strip is arranged in the supporting frame, a discharging pipe is fixed to the bottom of the mixing tank, and a smearing assembly is arranged at the bottom of the discharging pipe; the smearing assembly comprises a material guiding frame fixed to the bottom of the discharging pipe, sliding grooves are formed in the two ends of the material guiding frame, sliding blocks are arranged on the inner walls of the sliding grooves in a sliding mode, sealing plates attached to the inner walls of the two ends of the material guiding frame are fixed to one ends of the two sliding blocks, and smearing roller pieces are rotationally connected to the bottoms of one sides of the two sealing plates; the smearing roller piece has the stirring performance in the smearing process, a certain extrusion effect is formed on a reagent strip and a liquid absorption layer, the smearing roller piece better adapts to the surface of the reagent strip, smearing uniformity is guaranteed, and the preparation quality of the reagent strip is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of the preparation of europium-labeled antibodies, and particularly to a preparation device and a preparation method for a reagent strip of a europium-labeled antibody dilution solution. Background Art

[0002] A europium-labeled antibody is a biomolecular probe formed by conjugating and labeling the rare earth element europium with an antibody. Its principle is to utilize the unique fluorescence characteristics of europium, and through a specific chemical reaction, europium ions or their chelates are connected to the antibody molecule without affecting the immunoreactivity and specificity of the antibody. When irradiated with excitation light, europium emits characteristic fluorescence, which can be sensitively detected by a fluorescence detection instrument. In immunoassay techniques, europium-labeled antibodies are widely used in detection methods such as time-resolved fluorescence immunoassay due to their advantages of long fluorescence lifetime, large Stokes shift, and sharp emission spectrum. And the reagent strip of the europium-labeled antibody dilution solution, as a convenient and rapid detection tool, is of great significance for on-site detection, large-scale screening, etc.

[0003] Currently, there are many problems in the preparation process of traditional reagent strips of europium-labeled antibody dilution solutions: the existing preparation devices have low automation, and the manual operation steps are cumbersome, not only resulting in low preparation efficiency, but also easily introducing human errors. More importantly, during the coating process of loading the europium-labeled antibody dilution solution onto the reagent strip, it is difficult to precisely control the coating amount of the europium-labeled antibody dilution solution, resulting in uneven antibody concentration on the reagent strip, affecting the accuracy and repeatability of the detection results. Therefore, it is urgently necessary to propose a preparation device and a preparation method for a reagent strip of a europium-labeled antibody dilution solution to improve the above problems. Summary of the Invention

[0004] Aiming at the above-mentioned prior art, the technical problem to be solved by the present invention is that during the coating process of loading the europium-labeled antibody dilution solution onto the reagent strip, it is difficult to precisely control the coating amount of the europium-labeled antibody dilution solution, resulting in uneven antibody concentration on the reagent strip, affecting the accuracy and repeatability of the detection results.

[0005] To solve the above problems, the present invention provides a preparation device for a europium-labeled antibody dilution solution reagent strip, which includes a support frame. On one side of the top of the support frame, a mixing tank is installed, and a reagent strip is arranged inside the support frame. The bottom of the mixing tank is fixed with a discharge pipe, and a coating assembly is arranged at the bottom of the discharge pipe. The coating assembly includes a material guiding frame fixed at the bottom of the discharge pipe. Both ends of the material guiding frame are provided with sliding grooves, and sliders are slidably arranged on the inner walls of the sliding grooves. One end of each of the two sliders is fixed with a sealing plate attached to the inner walls at both ends of the material guiding frame, and a coating roller member is rotatably connected to the bottom of one side of the two sealing plates. The other end of one of the sliders is fixed with a lifting plate, and an electric push rod for adjusting the height of the lifting plate is installed on the outer wall of one end of the material guiding frame. The coating roller member includes a central shaft, and an anti-permeation layer is arranged on the outer wall of the central shaft. An absorbent layer is arranged on the outer wall of the anti-permeation layer. Soft rubber seats are arranged at equal distances between the inner wall of the anti-permeation layer and the outer wall of the central shaft. Multiple columns of E-shaped tubes are fixed at equal distances on the outer wall of the central shaft, and the positions of the E-shaped tubes are staggered with the positions of the soft rubber seats. The E-shaped tubes are filled with inert gas. Three inner walls of each E-shaped tube are inserted with movable rods, and one end of each movable rod is fixed with a support rod. A piston is fixed at one end of the outer wall of each movable rod. One end of the movable rod located in the middle position is installed with a spring at the middle position of the inner wall of the E-shaped tube.

[0006] The present invention is further arranged such that a liquid inlet hole communicating with the discharge pipe is opened at the middle position of the top of the material guiding frame, and L-shaped connecting plates are fixed on one side of the two sealing plates. Plugging plates attached to the bottom of the liquid inlet hole are fixed on the tops of the L-shaped connecting plates.

[0007] The present invention is further arranged such that the mixing tank is fixedly composed of an outer shell and an inner tank, and a stirrer is installed at the middle position of the mixing tank. The discharge pipe is fixed at the middle position of the bottom of the inner tank and extends out of the outer shell. The gap between the outside of the inner tank and the inside of the outer shell is designed as a heat preservation cavity.

[0008] The present invention is further arranged such that a drying box and a cleaning assembly are successively installed at the top of the support frame near the middle position. First side plates are fixed at both ends of one side of the drying box, and second side plates are fixed at both ends of one side of the cleaning assembly and the top of the support frame. One end of each of the two first side plates is rotatably connected to a first rotating shaft, and one end of the bottom of each of the two second side plates is rotatably connected to a second rotating shaft. Installation grooves are opened on both sides of the tops of the two second side plates, and a unwinder and a winder are respectively installed on the inner walls of the installation grooves. The reagent strip is wound around the unwinder, the winder, the first rotating shaft and the second rotating shaft.

[0009] The present invention is further arranged such that a support table is fixedly installed on one side of the two first side plates, and the support table is located directly below the material guiding frame. An arc-shaped groove is opened at the middle position of the top of the support table, and the arc-shaped groove is located directly below the coating roller member. A collection box is arranged inside the support frame, and the collection box is located below the first side plates and the support table.

[0010] The present invention is further configured such that the cleaning assembly includes a cleaning box installed on one side of the drying box and the top of the support frame, and a plurality of support rollers are rotatably connected to the inner wall of the cleaning box. The reagent strips are wound around the support rollers. Inlets and outlets are provided on both sides of the cleaning box and at the top and bottom of both sides of the drying box, and the reagent strips pass through the inlets and outlets. A drain pipe is fixed to the bottom of one side of the cleaning box, and a drain valve is installed at one end of the drain pipe.

[0011] The present invention is further configured such that two mounting holes are provided on one side of the cleaning box near the middle position, and the inner walls of the mounting holes are respectively rotatably connected to the transmission shafts of the connecting shafts through sealed bearings. One end of the transmission shaft is fixedly connected to one end of one of the support rollers. A cleaning roller brush is fixedly installed at one end of the connecting shaft, and the cleaning roller brush is attached to the reagent strip. Gears are installed at the other ends of the connecting shaft and the transmission shaft, and the two gears are meshed with each other.

[0012] The present invention is further configured such that a vacuum pump and a low-temperature heater are respectively installed on one side of the drying box, and an L-shaped square pipe is installed between the top side of the drying box and one side of the housing. The drying box, the L-shaped square pipe, and the heat preservation cavity are communicated.

[0013] The present invention is further configured such that a cutting machine is installed on the top side of the two second side plates close to the winder, and a cutting table is installed at the bottom of one side of the two second side plates. A cutting opening is provided at the middle position of the cutting table, and the cutting table is located directly below the cutting machine.

[0014] A preparation method of an europium-labeled antibody dilution solution reagent strip, which is applied to a preparation device of an europium-labeled antibody dilution solution reagent strip, includes the following steps:

[0015] Step 1: Obtain each component of the europium-labeled antibody dilution solution according to the following ratio: Tris-HCl buffer solution with a pH value of 7.2 - 7.6 and a concentration of 20 - 50 mmol / L; bovine serum albumin with a mass fraction of 0.5% - 2%; trehalose with a mass fraction of 1% - 5%; ProClin300 preservative with a volume fraction of 0.05% - 0.2%; and the rest is deionized water;

[0016] Step 2: Prepare a carrier: Select a nitrocellulose membrane as the carrier of the reagent strip;

[0017] Step 3: Dilute the solution: Inject each component of the prepared europium-labeled antibody dilution solution into the mixing tank, and mix them evenly for 20 - 30 min by a stirrer in the mixing tank;

[0018] Step 4: Evenly apply: turn off the agitator, wind the reagent strip around the entire preparation device, clean the surface of the reagent strip using the cleaning component, and then pass it through the bottom of the mixing tank after drying, and evenly apply the europium-labeled antibody reagent on the reagent strip through the application component;

[0019] Step 5: Continue drying: Pass the smeared test strip through a drying oven at a drying temperature of 45-60°C;

[0020] Step 6: Cutting and packaging: Cut the dried reagent strips into required lengths using a cutter, place the cut reagent strips into sealed bags, add desiccant, and perform vacuum packaging.

[0021] In summary, after adopting the above structure, the present invention has the following advantages compared with the prior art:

[0022] 1. In the present invention, the electric push rod in the smearing assembly can be used to adjust the height of the lifting plate, thereby controlling the position of the sealing plate and the smearing roller. When it is necessary to smear the reagent, the smearing roller can be lowered for smearing, and when it is not necessary, the sealing plate can be raised for sealing to prevent the reagent from dripping and contamination, thereby improving the controllability and accuracy of the smearing and facilitating the operation. Moreover, the liquid-absorbing layer in the smearing roller can effectively absorb and smear the reagent, and the anti-penetration layer can prevent the reagent from penetrating into the central axis, thereby extending the service life of the smearing roller. The internal soft rubber seat, E-shaped tube, movable rod, support rod, spring and other structures cooperate with the setting of the arc groove, so that the smearing roller has a toggling performance during the smearing process, forms a certain squeezing effect on the reagent strip and the liquid-absorbing layer, better adapts to the surface of the reagent strip, ensures the uniformity of the smearing, and improves the quality of the reagent strip preparation.

[0023] 2. In the present invention, by setting up the unwinder, winder, first rotating shaft, second rotating shaft and other components, the reagent strip can be smoothly transferred in the preparation device, and the whole preparation process is continuous and efficient. The reagent dripped during the preparation process is collected by the collection box to keep the inside of the device clean. The prepared reagent strip can be accurately cut by the setting of the cutting machine and the cutting table to obtain a reagent strip product that meets the specifications.

[0024] 3. In the present invention, the cleaning roller brush in the cleaning component is fitted with the reagent strip and rotated under the transmission of the gear, which can effectively clean the impurities on the surface of the reagent strip, ensure the cleanliness of the surface of the reagent strip, and improve the detection accuracy of the reagent strip. The cleaned reagent strip can be dried and heated in a drying oven, so that the reagent can be better infiltrated into the reagent strip when it is subsequently applied, thereby improving the performance and stability of the reagent strip. The reagent strip after being applied with the reagent can also be dried to fully evaporate the moisture in the reagent to form a dry reagent strip, thereby ensuring that the components in the reagent can be firmly attached to the nitrocellulose membrane.

[0025] 4. In the present invention, the L-shaped square tube connects the drying oven and the insulation chamber, which can reasonably utilize the heat during the drying process to heat the inside of the mixing tank, improve the mixing effect and energy utilization efficiency, and at the same time help to maintain the temperature stability of the reagent in the mixing tank.

[0026] 5. In the present invention, Tris-HCl buffer solution, bovine serum albumin, trehalose, ProClin300 preservative, and deionized water are used as the components of the dilution solution of the europium-labeled antibody, which can maintain the stability of the pH value of the solution, provide a suitable pH environment for the europium-labeled antibody, and can prevent the non-specific adsorption of the europium-labeled antibody under the action of bovine serum albumin, protect the antibody activity, and also have good moisture retention and the function of protecting biological macromolecules through the action of trehalose, which can enhance the stability of the europium-labeled antibody. In addition, the ProClin300 preservative can effectively inhibit the growth of microorganisms and extend the storage period of the reagent strip. Moreover, nitrocellulose membrane is selected as the carrier of the reagent strip, which has good adsorption performance and liquid flow rate, and can ensure the accuracy and sensitivity of the reagent strip during the detection process. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic three-dimensional structure diagram of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0028] Figure 2 It is a front view of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0029] Figure 3 It is a schematic structure diagram of the first rotating shaft and the second rotating shaft of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0030] Figure 4 It is a schematic structure diagram of the cleaning component of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0031] Figure 5 It is a schematic structure diagram of the installation groove of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0032] Figure 6 It is a schematic structure diagram of the outer shell and the inner liner of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0033] Figure 7 It is a schematic structure diagram of the material guiding frame and the electric push rod of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0034] Figure 8 It is a schematic structure diagram of the coating roller part and the plugging plate of a device for preparing a reagent strip of a dilution solution of a europium-labeled antibody according to the present invention;

[0035] Figure 9 Schematic diagram of the central axis and liquid absorption layer structure of a preparation device for a europium-labeled antibody dilution solution reagent strip according to the present invention;

[0036] Figure 10 Front sectional view of the coating roller part of a preparation device for a europium-labeled antibody dilution solution reagent strip according to the present invention.

[0037] Description of the reference numerals in the figure:

[0038] 1. Mixing tank; 101. Outer shell; 102. Inner tank; 103. Heat preservation cavity; 2. Support frame; 3. Coating assembly; 301. Feeding frame; 302. Electric push rod; 303. Lifting plate; 304. Chute; 305. Sealing plate; 306. L-shaped connecting plate; 307. Plugging plate; 308. Coating roller part; 3081. Central axis; 3082. Liquid absorption layer; 3083. Anti-permeation layer; 3084. Soft rubber seat; 3085. Support rod; 3086. E-shaped tube; 3087. Movable rod; 3088. Spring; 3089. Piston; 4. First side plate; 5. Collection box; 6. Drying oven; 7. Cleaning assembly; 701. Cleaning box; 702. Connecting shaft; 703. Gear; 704. Transmission shaft; 705. Cleaning roller brush; 8. Unwinder; 9. Second side plate; 10. Rewinder; 11. Cutting machine; 12. L-shaped square tube; 13. Vacuum pump; 14. Low-temperature heater; 16. Stirrer; 17. First rotating shaft; 18. Support table; 19. Cutting table; 20. Cutting port; 21. Second rotating shaft; 22. Inlet and outlet; 23. Support roller; 24. Drain valve; 25. Installation groove; 26. Arc groove; 27. Discharge pipe. Detailed implementation manners

[0039] The following details the embodiments of the present application. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application and should not be construed as a limitation to the present application.

[0040] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0041] In the description of the present application, it should be noted that, unless otherwise clearly specified and defined, the terms "installed", "connected", "coupled", "arranged" shall be understood in a broad sense. For example, it may be fixedly connected and arranged, or detachably connected and arranged, or integrally connected and arranged. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0042] Refer to Figures 1 - 10, the present invention provides a preparation device for a europium-labeled antibody dilution solution reagent strip, including a support frame 2. On one side of the top of the support frame 2, a mixing tank 1 is installed, and a reagent strip is arranged inside the support frame 2. A discharge pipe 27 is fixed to the bottom of the mixing tank 1. The mixing tank 1 is fixedly composed of an outer shell 101 and an inner liner 102, and a stirrer 16 is installed at the middle position of the mixing tank 1. The discharge pipe 27 is fixed at the middle position of the bottom of the inner liner 102 and extends out of the outer shell 101. The gap between the outside of the inner liner 102 and the inside of the outer shell 101 is designed as a heat preservation cavity 103. And a coating assembly 3 is arranged at the bottom of the discharge pipe 27. The coating assembly 3 includes a material guiding frame 301 fixed to the bottom of the discharge pipe 27. Both ends of the material guiding frame 301 are provided with sliding grooves 304. Sliders are slidably arranged on the inner walls of the sliding grooves 304. One end of each of the two sliders is fixed with a sealing plate 305 that fits against the inner walls at both ends of the material guiding frame 301. And a coating roller member 308 is rotatably connected to the bottom of one side of the two sealing plates 305. The other end of one of the sliders is fixed with a lifting plate 303. An electric push rod 302 for adjusting the height of the lifting plate 303 is installed on the outer wall of one end of the material guiding frame 301. The coating roller member 308 includes a central shaft 3081. And an anti-permeation layer 3083 is arranged on the outer wall of the central shaft 3081. An absorbent layer 3082 is arranged on the outer wall of the anti-permeation layer 3083. Soft rubber seats 3084 are arranged at equal distances between the inner wall of the anti-permeation layer 3083 and the outer wall of the central shaft 3081. Multiple columns of E-shaped tubes 3086 are fixedly arranged on the outer wall of the central shaft 3081 at equal distances. And the positions of the E-shaped tubes 3086 are staggered with the positions of the soft rubber seats 3084. The E-shaped tubes 3086 are filled with inert gas. Three inner walls of each E-shaped tube 3086 are inserted with movable rods 3087. One end of each movable rod 3087 is fixed with a support rod 3085. A piston 3089 is fixedly arranged at one end of the outer wall of each movable rod 3087. A spring 3088 is installed between the other end of the movable rod 3087 located in the middle position and the middle position of the inner wall of the E-shaped tube 3086. A liquid inlet hole communicating with the discharge pipe 27 is opened at the middle position of the top of the material guiding frame 301. And L-shaped connecting plates 306 are fixed to one side of the two sealing plates 305. Plugging plates 307 that fit against the bottom of the liquid inlet hole are fixed to the tops of the L-shaped connecting plates 306. With the above-mentioned coating roller member 308, the height of the lifting plate 303 is adjusted by the electric push rod 302 in the coating assembly 3, and the positions of the sealing plate 305 and the coating roller member 308 are lowered, so that the coating roller member 308 presses the reagent strip into the arc-shaped groove 26. As the reagent strip moves, it will drive the coating roller member 308 to rotate. At this time, the plugging plate 307 on one side of the sealing plate 305 leaves the liquid inlet hole, and the reagent enters the material guiding frame 301. The absorbent layer 3082 of the coating roller member 308 absorbs the reagent in the material guiding frame 301. Due to the actions of structures such as the soft rubber seats 3084, E-shaped tubes 3086, movable rods 3087, and support rods 3085, the coating roller member 308 can better fit the surface of the reagent strip. That is, since the E-shaped tubes 3086 are filled with inert gas, the air pressure in the E-shaped tubes 3086 remains stable at this time.When a certain movable rod 3087 is squeezed, other movable rods 3087 will move in the reverse direction under the action of air pressure. At this time, the squeezing liquid absorption layer 3082 is in better contact with the reagent strip, enabling the reagent to enter the reagent strip better.

[0043] In the present invention, a drying box 6 and a cleaning component 7 are successively installed at the top near the middle position of the support frame 2. Both ends on one side of the drying box 6 are fixed with first side plates 4. Both ends on one side of the cleaning component 7 and the top of the support frame 2 are fixed with second side plates 9. One end of two first side plates 4 is rotatably connected to a first rotating shaft 17, and one end of the bottom of two second side plates 9 is rotatably connected to a second rotating shaft 21. Installation grooves 25 are respectively formed on both sides of the top of two second side plates 9. A unwinder 8 and a winder 10 are respectively installed on the inner walls of the installation grooves 25. The reagent strip is wound around the unwinder 8, the winder 10, the first rotating shaft 17 and the second rotating shaft 21. As Figure 1 、 Figure 2 and Figure 3 shown, through the arrangement of components such as the unwinder 8, the winder 10, the first rotating shaft 17 and the second rotating shaft 21, the reagent strip can be smoothly transported in the preparation device, and the whole preparation process is continuous and efficient.

[0044] In the present invention, a support table 18 is fixedly installed on one side of two first side plates 4, and the support table 18 is located directly below the material guiding frame 301. An arc-shaped groove 26 is formed at the middle position of the top of the support table 18, and the arc-shaped groove 26 is located directly below the coating roller member 308. A collection box 5 is arranged inside the support frame 2, and the collection box 5 is located below the first side plates 4 and the support table 18. As Figure 1 and Figure 5 shown, by adopting the above-mentioned arc-shaped groove 26, when the position of the coating roller member 308 is lowered, the reagent strip can be driven to synchronously fit into the arc-shaped groove 26, increasing the contact area between the reagent strip and the coating roller member 308. On the one hand, the coating area and efficiency are improved. On the other hand, the friction force of the reagent strip on the coating roller member 308 is increased, facilitating the driving of the coating roller member 308 to rotate, and the reagent dripping during the preparation process is collected by the collection box 5 to keep the inside of the device clean.

[0045] In the present invention, the cleaning assembly 7 includes a cleaning box 701 installed on one side of the drying box 6 and the top of the support frame 2. A plurality of support rollers 23 are rotatably connected to the inner wall of the cleaning box 701. The reagent strips are wound around the support rollers 23. Inlets and outlets 22 are provided on both sides of the cleaning box 701 and at the top and bottom of both sides of the drying box 6. The reagent strips pass through the inlets and outlets 22. A drain pipe is fixed to the bottom of one side of the cleaning box 701, and a drain valve 24 is installed at one end of the drain pipe. Two mounting holes are provided on one side of the cleaning box 701 near the middle position. The inner walls of the mounting holes are respectively rotatably connected to a drive shaft 704 of a connecting shaft 702 through sealed bearings. One end of the drive shaft 704 is fixedly connected to one end of one of the support rollers 23. A cleaning roller brush 705 is fixedly installed at one end of the connecting shaft 702. The cleaning roller brush 705 is attached to the reagent strip. Gears 703 are installed at the other ends of the connecting shaft 702 and the drive shaft 704 respectively. The two gears 703 are meshed with each other. As Figure 2 and Figure 4 shown, with the above-mentioned cleaning assembly 7, when the reagent strip enters the cleaning box 701, the plurality of support rollers 23 in the cleaning box 701 cooperate with the reagent strip to rotate. At this time, due to the meshing of the gears 703 on the connecting shaft 702 and the drive shaft 704, the cleaning roller brush 705 rotates when the reagent strip moves, thereby cleaning the impurities on the surface of the reagent strip and avoiding the influence of impurities on the coating effect and subsequent test effect.

[0046] In the present invention, a vacuum pump 13 and a low-temperature heater 14 are respectively installed on one side of the drying box 6. An L-shaped square pipe 12 is installed between the top of one side of the drying box 6 and one side of the outer shell 101. The drying box 6, the L-shaped square pipe 12 and the heat preservation cavity 103 are communicated. As Figure 1 、 Figure 2 、 Figure 3 and Figure 6 shown, with the above-mentioned vacuum pump 13 and low-temperature heater 14, the air in the drying box 6 is pumped out by the vacuum pump 13 to form a vacuum environment, reducing the boiling point of water and accelerating the drying speed. At the same time, the low-temperature heater 14 provides an appropriate temperature to further promote the drying of the reagent strip. Under the action of the L-shaped square pipe 12, the drying box 6 and the heat preservation cavity 103 are communicated, and the heat during the drying process is reasonably utilized to heat the inside of the mixing tank 1, improving the mixing effect and energy utilization efficiency, and at the same time helping to maintain the temperature stability of the reagent in the mixing tank 1.

[0047] In the present invention, a cutting machine 11 is installed on the top of one side of the two second side plates 9 close to the winding machine 10. A cutting table 19 is installed at the bottom of one side of the two second side plates 9. A cutting opening 20 is provided at the middle position of the cutting table 19. The cutting table 19 is located directly below the cutting machine 11. As Figure 1 、 Figure 2 、and Figure 5As shown in the figure, by using the above-mentioned cutting machine 11 and cutting table 19, the dried reagent strips can be transported to the cutting machine 11, and the reagent strips are cut according to the specifications set by the cutting machine 11, and reagent strip products of europium-labeled antibody dilution solution meeting the requirements can be obtained.

[0048] A preparation method of a europium-labeled antibody dilution solution reagent strip, which is applied to a preparation device of a europium-labeled antibody dilution solution reagent strip, includes the following steps:

[0049] Step 1: Obtain each component of the europium-labeled antibody dilution solution according to the following ratio: Tris-HCl buffer solution, with a pH value of 7.2 - 7.6 and a concentration of 20 - 50 mmol / L; bovine serum albumin, with a mass fraction of 0.5% - 2%; trehalose, with a mass fraction of 1% - 5%; ProClin300 preservative, with a volume fraction of 0.05% - 0.2%; the rest is deionized water;

[0050] Step 2: Prepare the carrier: Select a nitrocellulose membrane as the carrier of the reagent strip;

[0051] Step 3: Dilute the solution: Inject each component of the prepared europium-labeled antibody dilution solution into the mixing tank 1, and mix evenly for 20 - 30 min by the stirrer 16 in the mixing tank 1;

[0052] Step 4: Uniformly coat: Turn off the stirrer 16, wind the reagent strip around the entire preparation device, clean the surface of the reagent strip by the cleaning component 7, and then pass through the bottom of the mixing tank 1 after drying, and uniformly coat the europium-labeled antibody reagent on the reagent strip by the coating component 3;

[0053] Step 5: Continue drying: Pass the coated reagent strip through the drying oven 6, and the drying temperature is 45 - 60 °C;

[0054] Step 6: Cut and package: Cut the dried reagent strip to the required length by the cutting machine 11, put the cut reagent strip into a sealed bag, add a desiccant, and perform vacuum packaging.

[0055] In summary, the working principle of the present invention: Inject each component into the mixing tank 1, stir the europium-labeled antibody dilution solution reagent in the mixing tank 1 by the stirrer 16 to ensure that the reagent is fully mixed evenly, and the mixed reagent flows to the coating component 3 through the discharge pipe 27;

[0056] The unwinder 8 unwinds the reagent strips without applying reagents. The reagent strips sequentially pass around the cleaning assembly 7, the drying oven 6, and the first rotating shaft 17, then return to the drying oven 6, pass around the second rotating shaft 21, and finally are wound by the rewinder 10. When the reagent strips enter the cleaning box 701, multiple support rollers 23 in the cleaning box 701 cooperate with the reagent strips to rotate. At this time, through the meshing action of the gears 703 on the connecting shaft 702 and the transmission shaft 704, the cleaning roller brush 705 rotates when the reagent strips move, thereby cleaning the impurities on the surface of the reagent strips. The sewage generated by cleaning is discharged through the drain pipe and the drain valve 24. The cleaned reagent strips enter the drying oven 6. The vacuum pump 13 pumps out the air in the drying oven 6 to form a vacuum environment, reducing the boiling point of water and accelerating the drying speed. At the same time, the low-temperature heater 14 provides an appropriate temperature to further promote the drying of the reagent strips. Moreover, the drying oven 6, the L-shaped square pipe 12, and the heat preservation cavity 103 are connected, and the heat during the drying process can be reasonably utilized to maintain the temperature stability of the reagent in the mixing tank 1 and accelerate the mixing efficiency;

[0057] When applying, the electric push rod 302 pushes the lifting plate 303 to descend, driving the connected slider, the sealing plate 305, and the coating roller member 308 to descend, so that the coating roller member 308 presses the reagent strip into the arc-shaped groove 26. As the reagent strip moves, it will drive the coating roller member 308 to rotate. At this time, the plugging plate 307 on one side of the sealing plate 305 leaves the liquid inlet hole, and the reagent enters the material guiding frame 301. The liquid absorbing layer 3082 of the coating roller member 308 absorbs the reagent in the material guiding frame 301. Due to the functions of the soft rubber seat 3084, the E-shaped pipe 3086, the movable rod 3087, the support rod 3085, and other structures, the coating roller member 308 can better fit the surface of the reagent strip. When the reagent strip moves, the liquid absorbing layer 3082 evenly applies the reagent on the reagent strip. After the application is completed, the electric push rod 302 drives the lifting plate 303 to rise, and the plugging plate 307 blocks the liquid inlet hole again to prevent the reagent from flowing out continuously;

[0058] The coated reagent strips continue to return to the drying oven 6. At this time, the dripping reagent will fall into the collection box 5 inside the support frame 2, and the drying oven 6 dries the reagent strips again, enabling the reagent to better penetrate into the reagent strips. Then, the dried reagent strips are transported to the cutting machine 11, and the cutting machine 11 cuts the reagent strips according to the set specifications, and finally obtains the europium-labeled antibody dilution solution reagent strip products that meet the requirements.

[0059] Combined with the current actual requirements, the above-mentioned implementation manner adopted in this application, the protection scope is not limited to this. Within the scope of knowledge possessed by those skilled in the art, various changes made without departing from the concept of this application still fall within the protection scope of the present invention.

Claims

1. A device for preparing a europium-labeled antibody dilution solution reagent strip, characterized in that: The invention comprises a support frame, a mixing tank is installed on one side of the top of the support frame, and a reagent strip is arranged in the support frame, a discharge pipe is fixed at the bottom of the mixing tank, and a smearing assembly is arranged at the bottom of the discharge pipe, the smearing assembly comprises a material guide frame fixed at the bottom of the discharge pipe, and slide grooves are provided at both ends of the material guide frame, and sliders are slid on the inner walls of the slide grooves, sealing plates are fixed at one end of the two sliders and are attached to the inner walls of the two ends of the material guide frame, and a smearing roller is rotatably connected to the bottom of one side of the two sealing plates, a lifting plate is fixed at the other end of one of the sliders, and a height adjusting mechanism for adjusting the lifting plate is installed on the outer wall of one end of the material guide frame An electric push rod, wherein the smear roller comprises a central axis, and an anti-permeation layer is arranged on the outer wall of the central axis, a liquid absorbing layer is arranged on the outer wall of the anti-permeation layer, and soft rubber seats are arranged on the inner wall of the anti-permeation layer and the outer wall of the central axis at equal distances, and a plurality of rows of E-shaped tubes at equal distances are fixed to the outer wall of the central axis, and the positions of the E-shaped tubes are staggered with the positions of the soft rubber seats, and the E-shaped tubes are filled with inert gas, and movable rods are inserted into the three inner walls of the E-shaped tubes, and a support rod is fixed at one end of the movable rod, and a piston is fixed at one end of the outer wall of the movable rod, and a spring is installed between the other end of the movable rod located in the middle position and the middle position of the inner wall of the E-shaped tube.

2. The device for preparing a europium-labeled antibody dilution solution reagent strip according to claim 1, characterized in that: A liquid inlet hole connected to the discharge pipe is provided in the middle of the top of the material guide frame, and an L-shaped connecting plate is fixed on one side of the two sealing plates, and a sealing plate fitted on the bottom of the liquid inlet hole is fixed on the top of the L-shaped connecting plate.

3. The device for preparing a europium-labeled antibody dilution solution reagent strip according to claim 2, characterized in that: The mixing tank is composed of an outer shell and an inner tank, and an agitator is installed in the middle of the mixing tank. The discharge pipe is fixed in the middle of the bottom of the inner tank and extends out of the outer shell. The gap between the outside of the inner tank and the inside of the outer shell is designed to form a heat preservation chamber.

4. The device for preparing a europium-labeled antibody dilution solution reagent strip according to claim 3, characterized in that: A drying box and a cleaning component are installed in sequence on the top of the support frame near the middle position, and first side panels are fixed at both ends of one side of the drying box, and second side panels are fixed at both ends of one side of the cleaning component and on the top of the support frame, one end of the two first side panels is rotatably connected to the first rotating shaft, and the bottom of one end of the two second side panels is rotatably connected to the second rotating shaft, and installation grooves are opened on both sides of the top of the two second side panels, and an unwinder and a winder are respectively installed on the inner walls of the installation grooves, and the reagent strip is wound around the unwinder, the winder, the first rotating shaft and the second rotating shaft.

5. The device for preparing a Europium-labeled antibody dilution solution reagent strip according to claim 4, characterized in that: A support platform is fixedly installed on one side of the two first side panels, and the support platform is located directly below the material guide frame. An arc groove is opened in the middle position of the top of the support platform, and the arc groove is located directly below the coating roller. A collection box is arranged inside the support frame, and the collection box is located below the first side panel and the support platform.

6. The device for preparing a Europium-labeled antibody dilution solution reagent strip according to claim 5, characterized in that: The cleaning assembly includes a cleaning box installed on one side of the drying box and on the top of the support frame, and the inner wall of the cleaning box is rotatably connected to a plurality of support rollers, and a reagent strip is wound around the support rollers. Inlet and outlet ports are provided on both sides of the cleaning box and on the top and bottom of both sides of the drying box, and the reagent strip passes through the inlet and outlet ports. A drain pipe is fixed to the bottom of one side of the cleaning box, and a drain valve is installed at one end of the drain pipe.

7. The device for preparing a europium-labeled antibody dilution solution reagent strip according to claim 6, characterized in that: Two mounting holes are provided on one side of the cleaning box near the middle position, and the inner walls of the mounting holes are rotatably connected to a transmission shaft with a connecting shaft through a sealed bearing, one end of the transmission shaft is fixedly connected to one end of one of the supporting rollers, a cleaning roller brush is fixedly installed on one end of the connecting shaft, the cleaning roller brush is attached to the reagent strip, and gears are installed on the other end of the connecting shaft and the other end of the transmission shaft, and the two gears are meshed with each other.

8. The device for preparing a Europium-labeled antibody dilution solution reagent strip according to claim 7, characterized in that: A vacuum pump and a low-temperature heater are respectively installed on one side of the drying box, and an L-shaped square tube is installed on one side of the top of the drying box and one side of the shell. The drying box, the L-shaped square tube and the insulation chamber are connected.

9. The device for preparing a europium-labeled antibody dilution solution reagent strip according to claim 8, characterized in that: A cutting machine is installed on one side of the top of the two second side plates close to the winder, and a cutting table is installed on the bottom of one side of the two second side plates. A cutting opening is opened in the middle of the cutting table, and the cutting table is located directly below the cutting machine.

10. A method for preparing a europium-labeled antibody dilution solution reagent strip, applied to the europium-labeled antibody dilution solution reagent strip preparation device as claimed in claim 9, characterized in that: The following steps are involved: Step 1, obtain the components of the europium-labeled antibody dilution solution according to the following ratio: Tris-HCl buffer, pH value 7.2-7.6, concentration 20-50mmol / L; bovine serum albumin, mass fraction 0.5%-2%; trehalose, mass fraction 1%-5%; ProClin300 preservative, volume fraction 0.05%-0.2%; the rest is deionized water; Step 2: Prepare the carrier: Select nitrocellulose membrane as the carrier of the reagent strip; Step 3, diluting the solution: inject all components of the prepared europium-labeled antibody dilution solution into the mixing tank and mix them for 20-30 minutes using the stirrer in the mixing tank; Step 4: Evenly apply: turn off the agitator, wind the reagent strip around the entire preparation device, clean the surface of the reagent strip using the cleaning component, and then pass it through the bottom of the mixing tank after drying, and evenly apply the europium-labeled antibody reagent on the reagent strip through the application component; Step 5: Continue drying: Pass the smeared test strip through a drying oven at a drying temperature of 45-60°C; Step 6: Cutting and packaging: Cut the dried reagent strips into required lengths using a cutter, place the cut reagent strips into sealed bags, add desiccant, and perform vacuum packaging.

Images