Magnetic separation device, sample analyzer and magnetic separation method

Abstract

The invention discloses a magnetic separation device, a sample analyzer and a magnetic separation method. The magnetic separation device comprises a first rotating part and a second rotating part, the center line of the rotating shaft of the first rotating part and the center line of the rotating shaft of the second rotating part are on the same straight line; the first rotating piece is provided with a first number of liquid injection levels, a third number of adsorption levels and a first number of liquid discharge levels, and the second rotating piece is provided with a second number of liquid injection levels, a fourth number of adsorption levels and a second number of liquid discharge levels; magnetic substances are arranged on the liquid discharge position and the adsorption position; the first number is larger than the second number, and the third number is smaller than the fourth number. Based on the mode, the cost of the magnetic separation device can be reduced.

Description

Technical Field

[0001] This application relates to the field of detection technologies, and particularly to a magnetic separation device, a sample analyzer, and a magnetic separation method. Background Art

[0002] Magnetic separation technology is to perform solid-liquid phase separation on magnetic particle complexes and reaction solutions. To ensure the effect of magnetic separation, during the separation process, it is necessary to perform multiple processes of liquid injection, mixing, and liquid suction on the magnetic particle complexes to achieve a good separation and cleaning effect, so as to obtain accurate test results.

[0003] The defect of the prior art is that when performing different types of sample detections, if magnetic separation and cleaning of the samples are required, different specifications of magnetic separation devices usually need to be set to adapt to the different cleaning times and different cleaning durations corresponding to different detection methods. Existing magnetic separation devices usually can only meet the requirements of magnetic separation and cleaning corresponding to one detection method at the same time. If the requirements of magnetic separation and cleaning corresponding to two or more detection methods need to be met at the same time, two or more magnetic separation devices need to be set in the sample analyzer, which makes the volume of the sample analyzer too large, and thus the cost of the magnetic separation device is relatively high. Summary of the Invention

[0004] The main technical problem to be solved by this application is how to reduce the cost of the magnetic separation device

[0005] To solve the above technical problem, the first technical solution adopted by this application is: a magnetic separation device, comprising: a first rotating member; a second rotating member, the center line of the rotating shaft of the first rotating member and the center line of the rotating shaft of the second rotating member are on the same straight line; the first rotating member has a first number of liquid injection positions, a third number of adsorption positions, and a first number of liquid discharge positions, and the second rotating member has a second number of liquid injection positions, a fourth number of adsorption positions, and a second number of liquid discharge positions; magnetic substances are provided at the liquid discharge positions and the adsorption positions; the first number is greater than the second number, and the third number is less than the fourth number.

[0006] Wherein, on the first rotating member, along the rotation direction of the first rotating member, first position combinations are sequentially arranged at intervals around the rotating shaft; in the first position combination, a liquid injection position, an adsorption position, and a liquid discharge position are sequentially arranged at intervals around the rotating shaft along the rotation direction of the first rotating member; on the second rotating member, second position combinations are sequentially arranged at intervals around the rotating shaft along the rotation direction of the second rotating member; in the second position combination, a liquid injection position, an adsorption position, and a liquid discharge position are sequentially arranged at intervals around the rotating shaft along the rotation direction of the second rotating member.

[0007] Wherein, the radius of the circle formed by connecting the liquid injection positions, the adsorption positions, and the liquid discharge positions on the first rotating member is different from the radius of the circle formed by connecting the liquid injection positions, the adsorption positions, and the liquid discharge positions on the second rotating member.

[0008] Among them, the first position combination includes a liquid injection position, a fifth number of adsorption positions, and a liquid discharge position, and the second position combination includes a liquid injection position, a sixth number of adsorption positions, and a liquid discharge position; the fifth number is less than the sixth number.

[0009] Among them, the rotating shaft of the first rotating member is the same as that of the second rotating member, and the first rotating member is fixedly connected to the second rotating member; the magnetic separation device further includes a control motor for controlling the first rotating member and the second rotating member to rotate synchronously around the rotating shaft; the first number is a first preset multiple of the second number; the first preset multiple is an integer multiple greater than 1; and / or, the total number of the second position combination is a second preset multiple of the total number of the first position combination; the total number of the first position combination is the sum value obtained by adding 2 to the fifth number, the total number of the second position combination is the sum value obtained by adding 2 to the sixth number, and the second preset multiple is an integer multiple greater than 1.

[0010] Among them, the first preset multiple is 2 times, and / or, the second preset multiple is 2 times.

[0011] The rotating shaft of the first rotating member is the same as that of the second rotating member, and the first rotating member is fixedly connected to the second rotating member; the magnetic separation device further includes a control motor for controlling the first rotating member and the second rotating member to rotate synchronously around the rotating shaft; the sum of twice the first number and the third number is equal to the sum of twice the second number and the fourth number.

[0012] Among them, magnetic substances are provided on one side of the adsorption position and the liquid discharge position on the first rotating member close to or away from the rotating shaft, on one side of the adsorption position and the liquid discharge position on the second rotating member close to the rotating shaft, and on one side of the adsorption position and the liquid discharge position on the second rotating member away from the rotating shaft.

[0013] To solve the above technical problems, the second technical solution adopted by this application is: a sample analyzer, including a reagent device, a sample adding device, an incubation device, and the above magnetic separation device.

[0014] To solve the above technical problems, the third technical solution adopted by this application is: a magnetic separation method, which is applied to the above magnetic separation device or the above sample analyzer; the magnetic separation method includes: obtaining a target container containing a sample; in response to the detection to be performed on the sample being chemiluminescence detection, moving the target container to the liquid injection position, the adsorption position, and the liquid discharge position of the first rotating member respectively, and performing liquid injection, adsorption, and liquid discharge respectively to complete the magnetic separation operation meeting the chemiluminescence detection standard; in response to the detection to be performed on the sample being flow cytometry fluorescence detection, moving the target container to the liquid injection position, the adsorption position, and the liquid discharge position of the second rotating member respectively, and performing liquid injection, adsorption, and liquid discharge respectively to complete the magnetic separation operation meeting the flow cytometry fluorescence detection standard.

[0015] The beneficial effects of the present application are as follows: Different from the prior art, in the technical solution of the present application, a first rotating member and a second rotating member with the axis centerlines on the same straight line are provided in the magnetic separation device. Both the first rotating member and the second rotating member have a liquid injection position, an adsorption position, and a liquid discharge position. Magnetic substances are provided at the liquid discharge position and the adsorption position. On the premise of the above structure, by making the number of liquid injection positions on the first rotating member greater than the number of liquid injection positions on the second rotating member, and making the number of liquid discharge positions on the first rotating member greater than the number of liquid discharge positions on the second rotating member, it can be ensured that the number of cleaning times for magnetic separation cleaning corresponding to the first rotating member based on one liquid injection position and one liquid discharge position is greater than that of the second rotating member. In addition, by making the number of adsorption positions on the first rotating member less than the number of adsorption positions on the second rotating member, it can be ensured that the cleaning duration of a single magnetic separation cleaning corresponding to the first rotating member is less than that of the second rotating member. Based on the above method, the magnetic separation device can perform magnetic separation cleaning operations with a longer cleaning duration and fewer cleaning times based on the first rotating member, and can also perform magnetic separation cleaning operations with a shorter cleaning duration and more cleaning times based on the second rotating member. That is to say, the same magnetic separation device can perform magnetic separation cleaning operations of different specifications according to different user requirements, without setting more than two magnetic separation devices, which can reduce the volume of the sample analyzer and the cost of the magnetic separation device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 is one of the top view structural diagrams of an embodiment of the magnetic separation device of the present application;

[0018] Figure 2 is the second of the top view structural diagrams of an embodiment of the magnetic separation device of the present application;

[0019] Figure 3 is the structural diagram of an embodiment of the liquid delivery module of the present application;

[0020] Figure 4 is the structural diagram of an embodiment of the sample analyzer of the present application;

[0021] Figure 5 is the flowchart of an embodiment of the magnetic separation method of the present application.

[0022] Reference numerals: first rotating member 11, second rotating member 12, waste liquid unit 201, waste liquid pump 202, liquid discharge needle 203, first reagent unit 204, first suction and discharge unit 205, substrate needle 206, second reagent unit 207, second suction and discharge unit 208, liquid injection needle 209, bottom liquid needle 210, sample analyzer 30, reagent device 31, sample adding device 32, incubation device 33, magnetic separation device 34. Detailed implementation manners

[0023] The following further describes the present application in detail in conjunction with the drawings and embodiments. It should be specifically noted that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only some embodiments of the present application rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0024] Referring to "embodiments" herein means that the specific features, structures or characteristics described in conjunction with the embodiments may be included in at least one embodiment of the present application. The phrase appears in various positions in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0025] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "set", "connected" and "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected, or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0026] The present application provides a magnetic separation device. Refer to Figure 1 and Figure 2 , Figure 1 which is one of the top view structural schematic diagrams of an embodiment of the magnetic separation device of the present application, Figure 2 is the second of the top view structural schematic diagrams of an embodiment of the magnetic separation device of the present application. As shown in Figure 1 and Figure 2 , the magnetic separation device includes a first rotating member 11, a second rotating member 12 and a control motor (not shown in the figure).

[0027] The center line of the rotating shaft of the first rotating member 11 and the center line of the rotating shaft of the second rotating member 12 are on the same straight line.

[0028] Among them, in one example, the rotating shafts of the first rotating member 11 and the second rotating member 12 are the same, and the first rotating member 11 and the second rotating member 12 are fixedly connected. The first rotating member 11 and the second rotating member 12 can rotate around the rotating shaft on the same straight line. Since the first rotating member 11 and the second rotating member 12 are fixedly connected, when the first rotating member 11 rotates, the second rotating member 12 can rotate at the same angular velocity, or when the second rotating member 12 rotates, the first rotating member 11 can rotate at the same angular velocity. That is to say, the first rotating member 11 and the second rotating member 12 can rotate synchronously at the same angular velocity during rotation, and the two remain relatively stationary. In another example, the rotating shafts of the first rotating member 11 and the second rotating member 12 are independent of each other, and the first rotating member 11 and the second rotating member 12 can rotate independently around their respective rotating shafts.

[0029] The first rotating member 11 and the second rotating member 12 can be configured with control motors, and the control motors can control the first rotating member 11 and the second rotating member 12 to rotate around their respective rotating shafts.

[0030] Specifically, the control motor can be a device for driving at least one of the first rotating member 11 and the second rotating member 12 to rotate, so as to be able to drive the first rotating member 11 and the second rotating member 12 to rotate synchronously.

[0031] The first rotating member 11 has a first number of liquid injection levels, a third number of adsorption positions, and a first number of liquid discharge levels. The second rotating member 12 has a second number of liquid injection levels, a fourth number of adsorption positions, and a second number of liquid discharge levels. Magnetic substances are provided on the liquid discharge levels and the adsorption positions. The first number is greater than the second number, and the third number is less than the fourth number.

[0032] Among them, as Figure 1 and Figure 2 shown, the first rotating member 11 is provided with a first number of liquid injection levels (A1 - A4), a third number of adsorption positions (B), and a first number of liquid discharge levels (C1 - C4). The liquid injection level A1 - 2 adsorption positions B - liquid discharge level C1 can be the stations that the reaction vessel on the first rotating member 11 has to pass through during a magnetic separation cleaning. The reaction vessel on the first rotating member 11 can be driven to first move to the liquid injection level A1 for liquid injection, then move through 2 adsorption positions B, and finally move to the liquid discharge level C1 for liquid discharge to complete a magnetic separation cleaning. Similarly, the liquid injection level A2 - 2 adsorption positions B - liquid discharge level C2, the liquid injection level A3 - 2 adsorption positions B - liquid discharge level C3, and the liquid injection level A4 - 2 adsorption positions B - liquid discharge level C4 are all the stations that the first rotating member 11 has to pass through corresponding to a magnetic separation cleaning.

[0033] The second rotating member 12 is provided with a second number of liquid injection levels (A5 - A6), a fourth number of adsorption positions (B), and a second number of liquid discharge levels (C5 - C6). The liquid injection level A5 - 6 adsorption positions B - liquid discharge level C5 can be the stations that the reaction vessel has to pass through during a single magnetic separation cleaning. The reaction vessel on the first rotating member 11 can be driven to first move to the liquid injection level A5 for liquid injection, then move through 6 adsorption positions B, and finally move to the liquid discharge level C5 for liquid discharge, completing a single magnetic separation cleaning. Similarly, the liquid injection level A6 - 6 adsorption positions B - liquid discharge level C6 are also all the stations that need to be passed through corresponding to a single magnetic separation cleaning on the second rotating member 12.

[0034] Since magnetic substances are provided at the liquid discharge levels (C1 - C6) and the adsorption positions B, and the first number is greater than the second number, and the third number is less than the fourth number, in one example, as Figure 1 and Figure 2 shown, the first rotating member 11 and the second rotating member 12 meet the above requirements.

[0035] When the rotation speed of the first rotating member 11 is uniform, among all the stations corresponding to a single magnetic separation cleaning on the first rotating member 11, the number of stations capable of magnetic adsorption is less than that on the second rotating member 12 corresponding to a single magnetic separation cleaning. As a result, the duration of a single magnetic separation cleaning corresponding to the first rotating member 11 is shorter than that corresponding to the second rotating member 12.

[0036] When the sum of twice the first number and the third number is equal to the sum of twice the second number and the fourth number, that is, when the total number of liquid injection levels, adsorption positions, and liquid discharge levels set on the first rotating member 11 is the same as that on the second rotating member 12, it is still possible to make the number of station combinations (all stations from a liquid injection level to the closest liquid discharge level along the rotation direction of the corresponding rotating member) corresponding to a single magnetic separation cleaning set on the first rotating member 11 more than that on the first rotating member 11. As a result, the number of magnetic separation cleanings after the first rotating member 11 rotates one week is more than that of the second rotating member 12 after it rotates one week.

[0037] In summary, when the rotation speed of the first rotating member 11 is uniform and the total number of liquid injection positions, adsorption positions, and liquid discharge positions provided on the first rotating member 11 is the same as the total number of liquid injection positions, adsorption positions, and liquid discharge positions provided on the second rotating member 12, when the first rotating member 11 and the second rotating member 12 need to perform magnetic separation cleaning, after one rotation, the duration of a single magnetic separation cleaning of the first rotating member 11 relative to the second rotating member 12 is shorter and the total number of magnetic separation cleaning times is larger. That is, based on the above method, the two rotating members in the same magnetic separation device can be enabled to have the ability to perform magnetic separation cleaning of two specifications.

[0038] For example, the first rotating member 11 can be used for chemiluminescence detection, and the second rotating member 12 can be used for flow cytometry fluorescence detection.

[0039] In the magnetic separation cleaning of flow cytometry fluorescence detection, since the response of magnetic beads is slow, a relatively long magnetic adsorption duration needs to be provided before liquid discharge. Otherwise, it is easy to discharge the magnetic beads during liquid discharge because the magnetic beads are not completely adsorbed, resulting in serious loss of magnetic beads and abnormal results. However, due to its low sensitivity, the number of magnetic separation cleaning times required is small. For example, the duration of a single magnetic separation cleaning corresponding to flow cytometry fluorescence detection is usually greater than 15 seconds, and the number of cleaning times is usually 1 - 3 times.

[0040] In the magnetic separation cleaning of chemiluminescence detection, since the response of magnetic beads is fast, adsorption can be completed with a relatively short adsorption duration. However, due to its high sensitivity, the number of magnetic separation cleaning times required is large. For example, the duration of a single magnetic separation cleaning corresponding to chemiluminescence detection is usually 1 - 3 seconds, and the number of cleaning times is usually 3 - 5 times.

[0041] Therefore, the reaction container containing the sample for chemiluminescence detection can be placed on the first rotating member 11, and the reaction container containing the sample for flow cytometry fluorescence detection can be placed on the second rotating member 12 to respectively meet the requirements of the corresponding single magnetic separation cleaning duration and the total number of magnetic separation cleaning times. In the same magnetic separation cleaning device, the magnetic separation cleaning corresponding to the corresponding detection method can be completed simultaneously or separately, avoiding the situation that a separate magnetic separation device must be configured for these two detection methods respectively, improving the utilization rate of the magnetic separation device, reducing the overall volume of the sample analyzer or other equipment where the magnetic separation device is located, and also reducing the cost of the magnetic separation device.

[0042] Different from the prior art, in the technical solution of the present application, a first rotating member and a second rotating member with the axis center lines of the rotating shafts on the same straight line are provided in the magnetic separation device. Both the first rotating member and the second rotating member have a liquid injection position, an adsorption position, and a liquid discharge position. Magnetic substances are provided at the liquid discharge position and the adsorption position. On the premise of the above structure, by making the number of liquid injection positions on the first rotating member greater than the number of liquid injection positions on the second rotating member, and making the number of liquid discharge positions on the first rotating member greater than the number of liquid discharge positions on the second rotating member, it can be ensured that the number of cleaning times for magnetic separation cleaning that the first rotating member can perform based on one liquid injection position and one liquid discharge position is greater than that of the second rotating member. In addition, by making the number of adsorption positions on the first rotating member less than the number of adsorption positions on the second rotating member, it can be ensured that the cleaning duration for a single magnetic separation cleaning corresponding to the first rotating member is less than that of the second rotating member. Based on the above method, the magnetic separation device can perform magnetic separation cleaning operations with a longer cleaning duration and fewer cleaning times based on the first rotating member, and can also perform magnetic separation cleaning operations with a shorter cleaning duration and more cleaning times based on the second rotating member. That is, the same magnetic separation device can perform magnetic separation cleaning operations of different specifications according to different user requirements, without setting more than two magnetic separation devices, which can reduce the volume of the sample analyzer and lower the cost of the magnetic separation device.

[0043] In one embodiment, on the first rotating member 11, along the rotation direction of the first rotating member 11, a first position combination is sequentially arranged at intervals around the rotating shaft.

[0044] In the first position combination, along the rotation direction of the first rotating member 11, a liquid injection position, an adsorption position, and a liquid discharge position are sequentially arranged at intervals around the rotating shaft.

[0045] On the second rotating member 12, along the rotation direction of the second rotating member 12, a second position combination is sequentially arranged at intervals around the rotating shaft.

[0046] In the second position combination, along the rotation direction of the second rotating member 12, a liquid injection position, an adsorption position, and a liquid discharge position are sequentially arranged at intervals around the rotating shaft.

[0047] Specifically, as Figure 1 and Figure 2 shown, the first rotating member 11 and the second rotating member 12 can rotate synchronously along the rotation direction D. The liquid injection position, the adsorption position, and the liquid discharge position do not rotate with the corresponding rotating member. The rotation of the corresponding rotating member can cause the reaction container containing the sample provided thereon to pass through the liquid injection position, the adsorption position, and the liquid discharge position along the rotation direction respectively, and perform liquid injection, adsorption, and liquid discharge respectively, so as to realize magnetic separation cleaning.

[0048] As Figure 1 and Figure 2As shown, on the first rotating member 11, there are provided a first number of liquid injection levels (A1 - A4), a third number of adsorption positions (B), and a first number of liquid discharge levels (C1 - C4). On the second rotating member 12, there are provided a second number of liquid injection levels (A5 - A6), a fourth number of adsorption positions (B), and a second number of liquid discharge levels (C5 - C6).

[0049] On each rotating member, along the rotation direction D, from one liquid injection level to the closest liquid discharge level, all the stations corresponding to one magnetic separation cleaning are included.

[0050] For example, as Figure 1 and Figure 2 shown, on the first rotating member 11, along the rotation direction D, all the stations from A1 to C1 are all the stations corresponding to one magnetic separation cleaning, all the stations from A2 to C2 are all the stations corresponding to one magnetic separation cleaning, all the stations from A3 to C3 are all the stations corresponding to one magnetic separation cleaning, and all the stations from A4 to C4 are all the stations corresponding to one magnetic separation cleaning. In one example, as Figure 1 and Figure 2 shown, on the first rotating member 11, a single magnetic separation cleaning with 2 adsorption positions can be performed, and the total number of times of this magnetic separation cleaning performed after one full rotation is 4 times.

[0051] As Figure 1 and Figure 2 shown, on the second rotating member 12, along the rotation direction D, all the stations from A5 to C5 are all the stations corresponding to one magnetic separation cleaning, and all the stations from A6 to C6 are all the stations corresponding to one magnetic separation cleaning. In one example, as Figure 1 and Figure 2 shown, on the second rotating member 12, a single magnetic separation cleaning with 6 adsorption positions can be performed, and the total number of times of this magnetic separation cleaning performed after one full rotation is 2 times.

[0052] In practical applications, taking the magnetic separation cleaning of a sample by sampling the first rotating member 11 as an example, the reaction vessel containing the sample can be first placed on the first rotating member 11, the first rotating member 11 is driven to rotate, the reaction vessel is first moved to the liquid injection level A1, the corresponding liquid injection needle is controlled to inject liquid into the reaction vessel, and then the reaction vessel is moved to successively pass through each adsorption position B between the liquid injection level A1 and the liquid discharge level C1, and then the reaction vessel is moved to the liquid discharge level C1, and the corresponding liquid discharge needle is controlled to discharge the liquid from the reaction vessel, so as to complete a single magnetic separation cleaning corresponding to one liquid injection level to one liquid discharge level. By analogy, from the liquid injection level A2 to the liquid discharge level C2, from the liquid injection level A3 to the liquid discharge level C3, from the liquid injection level A4 to the liquid discharge level C4 respectively correspond to one magnetic separation cleaning, from the liquid injection level A5 to the liquid discharge level C5, from the liquid injection level A6 to the liquid discharge level C6, that is, each of the above-mentioned first position combinations or the above-mentioned second position combinations is respectively used for one magnetic separation cleaning when the corresponding rotating member rotates.

[0053] Based on the above method, it is possible to sequentially set the liquid injection level, the adsorption position, and the liquid discharge level in each position combination along the rotation direction on the first rotating member 11 and the second rotating member 12, so as to respectively construct the workstations required for one magnetic separation cleaning in each position combination, and when the rotating member rotates, each position combination can be respectively used for one magnetic separation cleaning, improving the reliability of the magnetic separation device.

[0054] Optionally, in one example, the radius of the circle formed by connecting the liquid injection level, the adsorption position, and the liquid discharge level on the first rotating member 11 is different from the radius of the circle formed by connecting the liquid injection level, the adsorption position, and the liquid discharge level on the second rotating member 12.

[0055] Specifically, as Figure 1 and Figure 2 shown, adjacent two workstations in each of the liquid injection level, the adsorption position, and the liquid discharge level in the first rotating member 11 can be connected to form a corresponding circle, and adjacent two positions in each of the liquid injection level, the adsorption position, and the liquid discharge level in the second rotating member 12 can be connected to form a corresponding circle. Among them, the radius of the circle formed by connecting the liquid injection level, the adsorption position, and the liquid discharge level on the first rotating member 11 is not greater than the radius of the circle formed by connecting the liquid injection level, the adsorption position, and the liquid discharge level on the second rotating member 12.

[0056] Based on the above method, it is possible to make the workstations on the first rotating member 11 be arranged in a staggered manner with the workstations on the second rotating member 12 in the horizontal direction, avoiding the situation where a workstation on the first rotating member 11 overlaps with a workstation on the second rotating member 12 in the horizontal direction and must be arranged in upper and lower layers, reducing the thickness of the magnetic separation device in the vertical direction perpendicular to the horizontal direction, thereby reducing the volume of the magnetic separation device, improving the rationality of the layout of each workstation, and reducing the manufacturing cost of the magnetic separation device.

[0057] In another example, optionally, in one example, the radius of the circle formed by connecting the liquid injection positions, adsorption positions, and liquid discharge positions on the first rotating member 11 is the same as the radius of the circle formed by connecting the liquid injection positions, adsorption positions, and liquid discharge positions on the second rotating member 12.

[0058] Specifically, for the first rotating member 11, connecting adjacent two work positions among the liquid injection positions, adsorption positions, and liquid discharge positions can form corresponding circles, and for the second rotating member 12, connecting adjacent two positions among the liquid injection positions, adsorption positions, and liquid discharge positions can form corresponding circles. Among them, the radius of the circle formed by connecting the liquid injection positions, adsorption positions, and liquid discharge positions on the first rotating member 11 is the same as the radius of the circle formed by connecting the liquid injection positions, adsorption positions, and liquid discharge positions on the second rotating member 12.

[0059] The first rotating member 11 and the second rotating member 12 can be arranged in layers in the vertical direction perpendicular to the horizontal direction, and corresponding liquid injection needles and liquid discharge needles can be arranged in layers for liquid injection and liquid discharge. Based on the above method, the possibility of the liquid injection needle or the liquid discharge needle injecting or discharging liquid to non-target work positions due to the work positions on different rotating members being too close can be reduced, and the reliability of the magnetic separation device is improved.

[0060] Optionally, the first position combination includes one liquid injection position, a fifth number of adsorption positions, and one liquid discharge position, and the second position combination includes one liquid injection position, a sixth number of adsorption positions, and one liquid discharge position.

[0061] Wherein, the fifth number is less than the sixth number.

[0062] Specifically, as Figure 1 and Figure 2 shown, on the first rotating member 11, the fifth number of the first position combination is 2, and a single magnetic separation cleaning with 2 adsorption positions can be performed, and the total number of times of this magnetic separation cleaning after rotating one week is 4 times.

[0063] As Figure 1 and Figure 2 shown, on the second rotating member 12, the fifth number of the second position combination is 6, and a single magnetic separation cleaning with 6 adsorption positions can be performed, and the total number of times of this magnetic separation cleaning after rotating one week is 2 times.

[0064] Based on the above method, by making the fifth quantity less than the sixth quantity, it is possible to achieve a uniform rotation speed of the first rotating member 11, and when the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the first rotating member 11 is the same as the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the second rotating member 12, after the first rotating member 11 and the second rotating member 12 rotate for magnetic separation cleaning, the second rotating member 12 has a longer single-time magnetic separation cleaning duration relative to the first rotating member 11. Thus, the two rotating members in the same magnetic separation device have the ability to perform magnetic separation cleaning of two specifications, improving the applicable range of the magnetic separation cleaning device and thus enhancing the reliability of the magnetic separation device.

[0065] Further, the rotating shaft of the first rotating member 11 is the same as the rotating shaft of the second rotating member 12, and the first rotating member 11 is fixedly connected to the second rotating member 12.

[0066] The magnetic separation device further includes a control motor for controlling the synchronous rotation of the first rotating member 11 and the second rotating member 12 around the rotating shaft.

[0067] The first quantity is the first preset multiple of the second quantity. Among them, the first preset multiple is an integer multiple greater than 1.

[0068] And / or, the total quantity of the second position combinations is the second preset multiple of the total quantity of the first position combinations. Among them, the total quantity of the first position combinations is the sum value obtained by adding 2 to the fifth quantity, the total quantity of the second position combinations is the sum value obtained by adding 2 to the sixth quantity, and the second preset multiple is an integer multiple greater than 1.

[0069] Specifically, based on the above method, by making the first quantity the first preset multiple of the second quantity, it is possible to achieve a uniform rotation speed of the first rotating member 11, and when the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the first rotating member 11 is the same as the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the second rotating member 12, after the first rotating member 11 and the second rotating member 12 rotate for magnetic separation cleaning, the second rotating member 12 has a longer single-time magnetic separation cleaning duration relative to the first rotating member 11.

[0070] It is also possible to make the total quantity of the second position combinations the second preset multiple of the total quantity of the first position combinations, so that when the rotation speed of the first rotating member 11 is uniform and the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the first rotating member 11 is the same as the total quantity of the liquid injection positions, adsorption positions, and liquid discharge positions provided on the second rotating member 12, after the first rotating member 11 and the second rotating member 12 rotate for magnetic separation cleaning, the first rotating member 11 has more magnetic separation cleaning times relative to the second rotating member 12.

[0071] In practice, the first preset multiple can be 2 times, and / or, the second preset multiple can be 2 times.

[0072] Thus, two rotating parts in the same magnetic separation device are capable of performing magnetic separation cleaning of two specifications, improving the applicable range of the magnetic separation cleaning device, and thus enhancing the reliability of the magnetic separation device.

[0073] Furthermore, the magnetic separation device further includes an infusion motor, at least one liquid injection needle, and at least one liquid discharge needle.

[0074] The liquid injection needle can be arranged above the corresponding liquid injection position, and the liquid discharge needle can be arranged above the corresponding liquid discharge position.

[0075] The infusion motor can be used to control the liquid injection needle to move downward to inject liquid into the reaction vessel when the reaction vessel moves to the liquid injection position with the rotation of the rotating part, or to control the liquid injection needle to move downward to discharge liquid from the reaction vessel when the reaction vessel moves to the liquid discharge position with the rotation of the rotating part, or to control the corresponding liquid injection needle or liquid discharge needle to move upward to the initial position after the liquid injection or liquid discharge is completed.

[0076] Specifically, the liquid injection needles and liquid discharge needles configured for the first rotating part 11 and the second rotating part 12 can be controlled by the same infusion motor, realizing the control of the liquid injection needles and liquid discharge needles by the same infusion motor in different types of detections, reducing the number of infusion motors to be set, and lowering the cost of the magnetic separation device.

[0077] In one embodiment, twice the first quantity plus the third quantity is equal to twice the second quantity plus the fourth quantity. Herein, the number of all workstations on the first rotating part 11 is equal to the number of all workstations on the second rotating part 12.

[0078] The magnetic separation device further includes a control motor. The rotating shaft of the first rotating part 11 is the same as that of the second rotating part 12, and the first rotating part 11 and the second rotating part 12 are fixedly connected. The control motor is used to control the first rotating part 11 and the second rotating part 12 to rotate synchronously around the rotating shaft.

[0079] and / or,

[0080] The magnetic separation device further includes an infusion motor, at least one liquid injection needle, and at least one liquid discharge needle. The liquid injection needle can be arranged above the corresponding liquid injection position, and the liquid discharge needle can be arranged above the corresponding liquid discharge position. The infusion motor can be used to control the liquid injection needle to move downward to inject liquid into the reaction vessel when the reaction vessel moves to the liquid injection position with the rotation of the rotating part, or to control the liquid injection needle to move downward to discharge liquid from the reaction vessel when the reaction vessel moves to the liquid discharge position with the rotation of the rotating part, or to control the corresponding liquid injection needle or liquid discharge needle to move upward to the initial position after the liquid injection or liquid discharge is completed.

[0081] Specifically, the control motor can drive any one of the first rotating member 11 and the second rotating member 12 to rotate, so that the first rotating member 11 and the second rotating member 12 rotate together, realizing the control of the rotation of the rotating member in different types of detections by the same control motor.

[0082] The liquid injection needle and the liquid discharge needle configured on the first rotating member 11 and the second rotating member 12 can both be controlled by the same infusion motor, realizing the control of the liquid injection needle and the liquid discharge needle in different types of detections by the same infusion motor.

[0083] Based on the above method, not only can the number of control motors to be set be reduced, but also the number of infusion motors to be set can be reduced, reducing the cost of the magnetic separation device.

[0084] In an embodiment, magnetic substances are provided on one side of the adsorption position and the liquid discharge position on the first rotating member 11 close to or away from the rotating shaft, on one side of the adsorption position and the liquid discharge position on the second rotating member 12 close to the rotating shaft, and on one side of the adsorption position and the liquid discharge position on the second rotating member 12 away from the rotating shaft.

[0085] Specifically, the magnetic substance can specifically refer to a permanent magnet, or other types of substances that can provide magnetic adsorption force, which is not limited here.

[0086] For example, when placing the reaction container containing the sample for chemiluminescence detection on the first rotating member 11 and placing the reaction container containing the sample for flow cytometry fluorescence detection on the second rotating member 12, since the magnetic adsorption force required for flow cytometry fluorescence detection is greater than the magnetic adsorption force required for chemiluminescence detection, therefore, magnetic substances can be provided on both sides of the corresponding work positions (such as the adsorption position and the liquid discharge position) of the second rotating member 12 close to and away from the rotating shaft, and only on one side of the corresponding work positions (such as the adsorption position and the liquid discharge position) of the first rotating member 11 close to and away from the rotating shaft.

[0087] In an example, as Figure 1 and Figure 2 shown, the first rotating member 11 is the rotating member used for magnetic separation cleaning during chemiluminescence detection, the second rotating member 12 is the rotating member used for magnetic separation cleaning during flow cytometry fluorescence detection, and the radius of the circle formed by connecting the liquid injection position, the adsorption position, and the liquid discharge position on the first rotating member 11 is greater than the radius of the circle formed by connecting the liquid injection position, the adsorption position, and the liquid discharge position on the second rotating member 12.

[0088] The above magnetic substance can be arranged on one side of the first rotating member 11 close to the rotating shaft, that is, on one side of the first rotating member 11 close to the center of the circle formed thereby, and the above magnetic substance can be arranged on both sides of the second rotating member 12 close to and away from the rotating shaft, that is, on both sides of the second rotating member 12 close to and away from the center of the circle formed thereby.

[0089] Optionally, on the basis of arranging the above magnetic substance on one side of the first rotating member 11 close to the rotating shaft and arranging the above magnetic substance on both sides of the second rotating member 12 close to and away from the rotating shaft, part or all of the magnetic substance arranged on one side of the first rotating member 11 close to the rotating shaft can specifically be a part of the magnetic substance arranged on one side of the second rotating member 12 away from the rotating shaft.

[0090] Specifically, the sample in the reaction vessel on the first rotating member 11 can be magnetically adsorbed by the magnetic substance arranged between the first rotating member 11 and the second rotating member 12, and the sample in the reaction vessel on the second rotating member 12 can be magnetically adsorbed by the magnetic substance arranged between the first rotating member 11 and the second rotating member 12 and the magnetic substance arranged on one side of the second rotating member 12 close to the rotating shaft. Among them, part or all of the magnetic substance used for magnetically adsorbing the sample in the reaction vessel on the first rotating member 11 can be a part of the magnetic substance used for magnetically adsorbing the sample in the reaction vessel on the second rotating member 12, that is, this part of the magnetic substance is the common magnetic substance for adsorbing the reaction vessels on the first rotating member 11 and the second rotating member 12.

[0091] Based on the above method, the utilization rate of the magnetic substance can be improved. While ensuring that the magnetic adsorption effect of the magnetic substance is good, the amount of the magnetic substance to be arranged is reduced as much as possible, and the cost of the magnetic separation device is reduced.

[0092] Furthermore, the distance between the magnetic substance and its nearest liquid discharge position or adsorption position is not less than 0.5 mm and not more than 5 mm.

[0093] Based on the above method, it can be ensured that the magnetic substance has sufficient magnetic adsorption capacity, but it will not cause unexpected situations in magnetic separation cleaning due to too strong magnetic adsorption capacity. That is, the magnetic substance can have a more appropriate magnetic adsorption capacity relative to its nearest liquid discharge position or adsorption position, and the reliability of the magnetic separation device is improved.

[0094] In an embodiment, as Figure 1 shown, a substrate level F1 can also be arranged on the first rotating member 11, and a bottom liquid level F2 can also be arranged on the second rotating member 12.

[0095] See Figure 3 , Figure 3It is a schematic structural diagram of an embodiment of the liquid delivery module of the present application. As Figure 3 shown, the magnetic separation device further includes a liquid delivery module.

[0096] First, as Figure 3 shown, the liquid delivery module includes a waste liquid unit 201, six waste liquid pumps 202, and six drainage needles 203.

[0097] The waste liquid unit 201 can be connected to one end of the six waste liquid pumps through liquid paths respectively. The other end of the waste liquid pump is connected to the drainage needle 203. By inserting the drainage needle into the reaction vessel at the drainage position and then driving the waste liquid pump, the supernatant in the reaction vessel can be sucked into the waste liquid unit 201 to complete the drainage. The six drainage needles 203 can respectively correspond to the drainage positions C1 - C6 described in the previous embodiment one by one.

[0098] Second, as Figure 3 shown, the liquid delivery module includes a first reagent unit 204, a first suction and discharge unit 205, and a substrate needle 206.

[0099] The first reagent unit 204 can specifically be used to store the substrate for chemiluminescence detection. The first suction and discharge unit 205 can be used to suck the substrate from the first reagent unit 204 and discharge it to the substrate needle 206. The substrate needle can be used to extend into the reaction vessel at the substrate position F1 to discharge the substrate.

[0100] Third, as Figure 3 shown, the liquid delivery module includes a second reagent unit 207, a second suction and discharge unit 208, six injection needles 209, and a bottom liquid needle 210.

[0101] The second reagent unit 207 can specifically be used to store the reagents for the bottom liquid of flow - through fluorescence detection and the cleaning liquid for magnetic separation cleaning. The second suction and discharge unit 208 can be used to suck the substrate from the second reagent unit 207 and discharge it to the injection needles 209 and the bottom liquid needle 210. The bottom liquid needle 210 can be used to extend into the reaction vessel at the substrate position F1 to discharge the substrate, and the injection needles 209 can be used to extend into the reaction vessels at the injection positions to discharge the cleaning liquid. The six injection needles 209 can respectively correspond to the injection positions A1 - A6 described in the previous embodiment one by one.

[0102] Optionally, a cup transfer position E1 can also be provided on the first rotating member 11, and a cup transfer position E2 can also be provided on the second rotating member 12.

[0103] When moving the reaction vessel onto the first rotating member 11, the corresponding gripper can be controlled to move the reaction vessel to the cup transfer position E1. When moving the reaction vessel out of the first rotating member 11, the reaction vessel can be moved to the cup transfer position E1 for the corresponding gripper to grab and remove.

[0104] It should be noted that the injection liquid level, adsorption position, drainage liquid level, substrate position, bottom liquid level, and cup transfer position are all positions on the rotating part, which can make the total number of positions set on a single rotating part be 18, so that the included angle formed by the connecting lines between the centers of the circles formed by any two adjacent positions and the center of the circle of the positions of the rotating part is 20 degrees. It can also be arranged in other ways, as long as it has the ability to enable the reaction vessels on the rotating part to sequentially pass through all the positions on the corresponding rotating part when the rotating part rotates.

[0105] In the process of magnetic separation cleaning, taking chemiluminescence detection as an example, the reaction vessel can be moved to the cup transfer position, and the first rotating part 11 is controlled to rotate so that the reaction vessel sequentially passes through multiple first position combinations to complete multiple magnetic separation cleanings. After the cleaning is completed, it is determined whether there is still a magnetic separation cleaning step in the subsequent steps. If not, the reaction vessel is moved to the substrate position, the substrate is added, and then the reaction vessel is moved to the cup transfer position for the gripper to grasp and remove for subsequent related operations. If there is, the reaction vessel is moved to the cup transfer position for the gripper to grasp and remove for subsequent related operations.

[0106] Taking flow cytometry fluorescence detection as an example, the reaction vessel can be moved to the cup transfer position, and the second rotating part 12 is controlled to rotate so that the reaction vessel sequentially passes through multiple second position combinations to complete multiple magnetic separation cleanings. After the cleaning is completed, it is determined whether there is still a magnetic separation cleaning step in the subsequent steps. If not, the reaction vessel is moved to the substrate position, the bottom liquid is added, and then the reaction vessel is moved to the cup transfer position for the gripper to grasp and remove for subsequent related operations. If there is, the reaction vessel is moved to the cup transfer position for the gripper to grasp and remove for subsequent related operations.

[0107] This application also proposes a sample analyzer. Refer to Figure 4 , Figure 4 is a schematic structural diagram of an embodiment of the sample analyzer of this application. As Figure 4 shown, the sample analyzer 30 includes a reagent device 31, a sample adding device 32, an incubation device 33, and a magnetic separation device 34. The magnetic separation device 34 can specifically be the magnetic separation device described in any of the previous embodiments, which will not be elaborated here.

[0108] The reaction vessel can be placed in the reagent device 31 to add the reagents required for detection, the reaction vessel can be placed in the sample adding device 32 to add the samples required for detection, the reaction vessel added with reagents and samples can be placed in the incubation device 33 for incubation, and the reaction vessel can be placed in the magnetic separation device 34 for magnetic separation cleaning.

[0109] Different from the prior art, in the technical solution of the present application, a first rotating member and a second rotating member with their axis centerlines on the same straight line are provided in the magnetic separation device. Both the first rotating member and the second rotating member have a liquid injection position, an adsorption position, and a liquid discharge position. Magnetic substances are provided at the liquid discharge position and the adsorption position. On the premise of the above structure, by making the number of liquid injection positions on the first rotating member greater than the number of liquid injection positions on the second rotating member, and making the number of liquid discharge positions on the first rotating member greater than the number of liquid discharge positions on the second rotating member, it can be ensured that the number of magnetic separation cleaning times that the first rotating member can perform based on one liquid injection position and one liquid discharge position is greater than that of the second rotating member. In addition, by making the number of adsorption positions on the first rotating member less than the number of adsorption positions on the second rotating member, it can be ensured that the cleaning duration of a single magnetic separation cleaning corresponding to the first rotating member is less than that of the second rotating member. Based on the above method, the magnetic separation device can perform magnetic separation cleaning operations with a longer cleaning duration and fewer cleaning times based on the first rotating member, and can also perform magnetic separation cleaning operations with a shorter cleaning duration and more cleaning times based on the second rotating member. That is to say, the same magnetic separation device can perform magnetic separation cleaning operations of different specifications according to different user requirements, without setting more than two magnetic separation devices, which can reduce the volume of the sample analyzer and the cost of the magnetic separation device.

[0110] In one embodiment, the sample analyzer further includes a flow cell, a flow-through cell, a liquid delivery module, a light source, a photoelectric sensor, and a control module.

[0111] The flow cell can be used to form a sheath flow, so that in the sample flow clamped in the middle of the sheath flow, the particles of the sample can flow through one by one for the detection beam emitted by the light source to irradiate one by one.

[0112] The flow-through cell can be used to temporarily store the sample that has undergone chemiluminescence detection processing, so that chemiluminescence occurs in the flow-through cell at this place.

[0113] The liquid delivery module is used to collect the sample and transport the sample to the flow cell or the flow-through cell. Among them, the liquid delivery module can be respectively connected to the flow cell and the flow-through cell, and can control the liquid delivery module to collect the sample and transport the collected sample to the flow cell or to the flow-through cell according to user needs.

[0114] The light source is used to emit a detection beam to the sample in the flow cell to excite the sample to generate a first beam. Specifically, the light source can be a laser light source or other types of light sources, which is not limited here. By emitting a detection beam to the particles in the flow cell, corresponding fluorescence can be excited, that is, a first beam is excited.

[0115] The photoelectric sensor is used to receive the first light beam and / or the second light beam emitted by the sample in the flow cell. Wherein, the photoelectric sensor can be located on the common optical path of the first light beam and the second light beam to receive and convert the first light beam when it is generated, or receive and convert the second light beam when it is generated, so as to generate a corresponding electrical signal, and the electrical signal is used for signal processing to obtain the sample detection result and complete the sample detection.

[0116] The control module is used for:

[0117] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the liquid delivery module to deliver the sample to the flow chamber, control the light source to emit a detection light beam to generate the first light beam, and output the detection result based on the electrical signal generated by the photoelectric sensor receiving the first light beam.

[0118] In response to the detection to be performed on the sample being chemiluminescence detection, control the liquid delivery module to deliver the sample to the flow cell so that the sample emits the second light beam in the flow cell, and output the detection result based on the electrical signal generated by the photoelectric sensor receiving the second light beam.

[0119] In one example, the optical path of the first light beam emitted to the photoelectric sensor and the optical path of the second light beam emitted to the photoelectric sensor at least partially overlap.

[0120] Based on the above method, it is possible to separately implement flow cytometry fluorescence detection and chemiluminescence detection in the same sample detection device, and use the same photoelectric sensor for the light beams under the two detections, reducing the cost and complexity of the detection steps and improving the reliability of the sample detection device.

[0121] Optionally, the sample analyzer further includes a signal switcher, which is connected to the photoelectric sensor and is used to connect the photoelectric sensor to the first circuit or the second circuit.

[0122] The first circuit is used to convert the received electrical signal into an analog signal.

[0123] The second circuit is used to convert the received electrical signal into a digital signal.

[0124] The control module is used for:

[0125] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the signal switcher to connect to the first circuit and output the detection result based on the analog signal output by the first circuit.

[0126] In response to the detection to be performed on the sample being chemiluminescence detection, control the signal switcher to connect to the second circuit and output the detection result based on the digital signal output by the second circuit.

[0127] Among them, the flow cell, flow-through cell, light source, photoelectric sensor, and the optical path between the light source and the photoelectric sensor are located in the same darkroom.

[0128] Specifically, the first circuit is used to convert the received electrical signal into an analog signal. Among them, the first circuit can be used to convert the electrical signal output by the photoelectric sensor after photoelectric conversion into an analog signal. It should be noted that when performing flow cytometry fluorescence detection, the electrical signal is usually converted into an analog signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0129] The second circuit is used to convert the received electrical signal into a digital signal. Among them, the second circuit can be used to convert the electrical signal output by the photoelectric sensor after photoelectric conversion into a digital signal. It should be noted that when performing chemiluminescence detection, the electrical signal is usually converted into a digital signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0130] In addition, by making the flow cell, flow-through cell, light source, photoelectric sensor, and the optical path between the light source and the photoelectric sensor located in the same darkroom, the setting of the darkroom can be reduced, and the anti-interference ability of the optical path can be improved, avoiding the negative impact of external light on the optical path where the first beam or the second beam is located, and improving the reliability of the sample detection device.

[0131] This application also proposes a magnetic separation method, which is applied to the magnetic separation device or sample analyzer described in any of the foregoing embodiments.

[0132] See Figure 5 , Figure 5 is a schematic flowchart of an embodiment of the magnetic separation method of this application. As Figure 5 shown, the magnetic separation method includes:

[0133] Step S41: Obtain a target container containing a sample.

[0134] Step S42: In response to the detection to be performed on the sample being chemiluminescence detection, move the target container to the liquid injection position, adsorption position, and liquid discharge position of the first rotating member respectively, and perform liquid injection, adsorption, and liquid discharge respectively to complete the magnetic separation operation meeting the chemiluminescence detection standard.

[0135] Step S43: In response to the detection to be performed on the sample being flow cytometry fluorescence detection, move the target container to the liquid injection position, adsorption position, and liquid discharge position of the second rotating member respectively, and perform liquid injection, adsorption, and liquid discharge respectively to complete the magnetic separation operation meeting the flow cytometry fluorescence detection standard.

[0136] Different from the prior art, in the technical solution of the present application, a first rotating member and a second rotating member with their axis centerlines on the same straight line are provided in the magnetic separation device. Both the first rotating member and the second rotating member have a liquid injection position, an adsorption position, and a liquid discharge position. Magnetic substances are provided at the liquid discharge position and the adsorption position. On the premise of the above structure, by making the number of liquid injection positions on the first rotating member greater than the number of liquid injection positions on the second rotating member, and making the number of liquid discharge positions on the first rotating member greater than the number of liquid discharge positions on the second rotating member, it can be ensured that the number of magnetic separation cleaning times that the first rotating member can perform based on one liquid injection position and one liquid discharge position is greater than that of the second rotating member. In addition, by making the number of adsorption positions on the first rotating member less than the number of adsorption positions on the second rotating member, it can be ensured that the cleaning duration of a single magnetic separation cleaning corresponding to the first rotating member is less than that of the second rotating member. Based on the above method, the magnetic separation device can perform magnetic separation cleaning operations with a longer cleaning duration and fewer cleaning times based on the first rotating member, and can also perform magnetic separation cleaning operations with a shorter cleaning duration and more cleaning times based on the second rotating member. That is to say, the same magnetic separation device can perform magnetic separation cleaning operations of different specifications according to different user requirements, without setting more than two magnetic separation devices, which can reduce the volume of the sample analyzer and the cost of the magnetic separation device.

[0137] In the description of the present application, the descriptions referring to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0138] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" can explicitly or implicitly include at least one such feature. In the description of the present application, "a plurality of" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0139] Any process or method description represented in a flowchart or otherwise described herein can be understood to represent a module, segment, or portion of code including one or more executable instructions for implementing a specific logical function or process. The scope of the preferred embodiments of the present application includes additional implementations where functions may be executed not in the order shown or discussed, including in a substantially simultaneous manner according to the relevant functions or in a reverse order, which should be understood by those skilled in the art to which the embodiments of the present application pertain.

[0140] The logic and / or steps represented in a flowchart or otherwise described herein, for example, can be considered a sequenced list of executable instructions for implementing a logical function and can be embodied specifically in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device (which can be a personal computer, server, network device, or other system that can fetch and execute instructions from the instruction execution system, apparatus, or device). For the purposes of this specification, a "computer-readable medium" can be any device that can contain, store, communicate, propagate, or transport a program for use by or in connection with an instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium include the following: an electrical connection having one or more wirings (electronic device), a portable computer diskette (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or Flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium can even be paper or other suitable medium on which the program can be printed, as the program can be obtained electronically, for example, by optically scanning the paper or other medium, followed by editing, interpretation, or other appropriate processing as necessary, and then storing it in a computer memory.

[0141] The above are only the embodiments of the present application and do not limit the patent scope of the present application. Any equivalent structural or equivalent process transformation made by using the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present application.

Claims

1. A magnetic separation device, characterized in that, Comprising: A first rotating member; A second rotating member, the center line of the rotating shaft of the first rotating member and the center line of the rotating shaft of the second rotating member being on the same straight line; Wherein, the first rotating member has a first number of liquid injection positions, a third number of adsorption positions, and a first number of liquid discharge positions, and the second rotating member has a second number of liquid injection positions, a fourth number of adsorption positions, and a second number of liquid discharge positions; Magnetic substances are provided at the liquid discharge positions and the adsorption positions; The first number is greater than the second number, and the third number is less than the fourth number.

2. The magnetic separation device according to claim 1, characterized in that On the first rotating member, along the rotation direction of the first rotating member, first position combinations are sequentially arranged at intervals around the rotating shaft; In the first position combination, along the rotation direction of the first rotating member, the liquid injection position, the adsorption position, and the liquid discharge position are sequentially arranged at intervals around the rotating shaft; On the second rotating member, along the rotation direction of the second rotating member, second position combinations are sequentially arranged at intervals around the rotating shaft; In the second position combination, along the rotation direction of the second rotating member, the liquid injection position, the adsorption position, and the liquid discharge position are sequentially arranged at intervals around the rotating shaft.

3. The magnetic separation device according to claim 2, characterized in that, The radius of the circle formed by connecting the liquid injection position, the adsorption position, and the liquid discharge position on the first rotating member is different from the radius of the circle formed by connecting the liquid injection position, the adsorption position, and the liquid discharge position on the second rotating member.

4. The magnetic separation device according to claim 2, wherein, The first position combination includes one liquid injection position, a fifth number of adsorption positions, and one liquid discharge position, and the second position combination includes one liquid injection position, a sixth number of adsorption positions, and one liquid discharge position; Wherein, the fifth number is less than the sixth number.

5. The magnetic separation device according to claim 4, characterized in that, The rotating shaft of the first rotating member is the same as the rotating shaft of the second rotating member, and the first rotating member is fixedly connected to the second rotating member; The magnetic separation device further includes a control motor for controlling the first rotating member and the second rotating member to rotate synchronously around the rotating shaft; The first number is a first preset multiple of the second number; wherein, the first preset multiple is an integer multiple greater than 1; And / or, the total number of the second position combinations is a second preset multiple of the total number of the first position combinations; wherein, the total number of the first position combinations is the sum value obtained by adding 2 to the fifth number, the total number of the second position combinations is the sum value obtained by adding 2 to the sixth number, and the second preset multiple is an integer multiple greater than 1.

6. The magnetic separation device according to claim 5, wherein The first preset multiple is 2 times, and / or, the second preset multiple is 2 times.

7. The magnetic separation device according to claim 1, wherein, The rotating shaft of the first rotating member is the same as the rotating shaft of the second rotating member, and the first rotating member is fixedly connected to the second rotating member; The magnetic separation device further includes a control motor for controlling the first rotating member and the second rotating member to rotate synchronously around the rotating shaft; The sum of twice the first number and the third number is equal to the sum of twice the second number and the fourth number.

8. The magnetic separation device according to any one of claims 1 to 7, characterized in that The magnetic substances are provided on one side of the adsorption position and the liquid discharge position on the first rotating member close to or away from the rotating shaft, on one side of the adsorption position and the liquid discharge position on the second rotating member close to the rotating shaft, and on one side of the adsorption position and the liquid discharge position on the second rotating member away from the rotating shaft.

9. A sample analyzer, characterized in that, It includes a reagent device, a sample adding device, an incubation device, and the magnetic separation device according to any one of claims 1 to 8.

10. A magnetic separation method, characterized in that, The magnetic separation method is applied to the magnetic separation device according to any one of claims 1 to 8, or the sample analyzer according to claim 9; The magnetic separation method includes: Obtaining a target container containing a sample; In response to the detection to be performed on the sample being chemiluminescence detection, the target container is respectively moved to the liquid injection position, the adsorption position, and the liquid discharge position of the first rotating member, and liquid injection, adsorption, and liquid discharge are respectively performed to complete the magnetic separation operation meeting the chemiluminescence detection standard; In response to the detection to be performed on the sample being flow cytometry fluorescence detection, the target container is respectively moved to the liquid injection position, the adsorption position, and the liquid discharge position of the second rotating member, and liquid injection, adsorption, and liquid discharge are respectively performed to complete the magnetic separation operation meeting the flow cytometry fluorescence detection standard.

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