Ultrafiltration purification and concentration device and process for herpes zoster vaccine production system

Abstract

The present invention relates to the technical field of herpes zoster vaccine production, specifically to an ultrafiltration purification and concentration device and process for a herpes zoster vaccine production system, which mainly includes a concentration tank, wherein an ultrafiltration membrane is arranged inside the concentration tank, and further includes: a vibration mechanism, wherein the vibration mechanism is arranged inside the concentration tank, and a stirring mechanism, wherein the stirring mechanism is arranged inside the concentration tank. By turning on a motor, a rotating shaft and a fixed bar are driven to rotate, the fixed bar drives a movable sleeve and a roller to rotate, and the roller rotates back and forth under the action of a protrusion and an elastic member, and the roller drives the support bar and the movable sleeve to move back and forth, drives the fixed rod and the sliding sleeve to move back and forth, and when the sliding sleeve moves left and right, it moves up and down on the outer wall of the fixed rod, and the sliding sleeve drives a knocking rod to rotate back and forth through a hinge block, and the knocking rod drives a knocking head to knock and vibrate the fixed shell back and forth, and the fixed shell drives the ultrafiltration membrane to vibrate, so that a gel layer is not easily generated on the surface of the ultrafiltration membrane, and the holes of the ultrafiltration membrane are not easily clogged, thereby improving the filtration efficiency of the ultrafiltration membrane and the production efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of herpes zoster vaccine production, in particular to an ultrafiltration purification and concentration device of a herpes zoster vaccine production system and a process thereof. Background Art

[0002] The shingles vaccine is mainly used to prevent shingles and its complications. This vaccine is made by using attenuated varicella-zoster virus or through DNA recombination technology, which can stimulate the human body to produce immunity against this virus. During the production process of the shingles vaccine, the shingles vaccine needs to be filtered and concentrated through an ultrafiltration purification and concentration device to obtain the required shingles vaccine.

[0003] When ultrafiltration and concentration of the shingles vaccine is carried out, the harvested liquid of the cell-expressed protein in the shingles vaccine contains a large amount of cell debris and impurities during the ultrafiltration process, which easily forms a gel layer on the surface of the ultrafiltration membrane. These gel layers easily adhere to the surface of the ultrafiltration membrane, causing blockage of the pores of the ultrafiltration membrane, making it difficult for part of the liquid to pass through the ultrafiltration membrane, resulting in a decrease in the filtration efficiency of the ultrafiltration membrane and a reduction in production efficiency. Summary of the Invention

[0004] The object of the present invention is to provide an ultrafiltration purification and concentration device for a herpes zoster vaccine production system to solve the problems raised by the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] An ultrafiltration purification and concentration device for a herpes zoster vaccine production system includes: a concentration tank, a tank cover installed on the top surface of the concentration tank, a mounting block fixedly installed on the outer wall of the concentration tank, a drain pipe and a water inlet pipe fixedly installed through the outer wall of the concentration tank, an ultrafiltration membrane installed inside the concentration tank, and further comprising:

[0007] A vibration mechanism is provided inside the concentration tank. The vibration mechanism includes a rotating shaft located inside the concentration tank. Two fixed sleeves and two movable sleeves are provided below the rotating shaft. A knocking head is provided below the movable sleeve. The vibration mechanism causes the ultrafiltration membrane to vibrate.

[0008] The stirring mechanism is arranged inside the concentration tank. The stirring mechanism includes a fixed plate and a stirring column located below the rotating shaft. The stirring mechanism is used to stir the liquid.

[0009] Preferably, the concentration tank is connected to the tank cover through a connecting assembly, a drainage pipe is fixedly installed on the bottom surface of the concentration tank, a fixed shell is fixedly installed on the inner wall of the concentration tank, the inner wall of the fixed shell is slidingly connected to the outer wall of the ultrafiltration membrane, a pressure ring is installed on the edge of the top surface of the ultrafiltration membrane, and the top surface of the pressure ring is fixedly connected to the top surface of the fixed shell by bolts.

[0010] Preferably, a boost pipe and an exhaust pipe are fixedly installed through the top surface of the tank cover, and a pressure gauge is fixedly installed through the top surface of the tank cover.

[0011] Preferably, a motor box is fixedly installed on the bottom surface of the tank cover by bolts, a motor is fixedly installed on the inner wall of the motor box, the upper end of the rotating shaft rotates through the bottom surface of the motor box and extends to the inside of the motor box, and the upper end of the rotating shaft is fixedly connected to the output end of the motor.

[0012] Preferably, a fixing bar is fixedly installed at the lower end of the rotating shaft, the outer wall of the fixing bar is fixedly connected to the inner walls of the two fixing sleeves, and the inner walls of the two movable sleeves are slidably connected to the outer wall of the fixing bar.

[0013] Preferably, two sliding rods are installed on the side of the movable sleeve for sliding through, one end of the two sliding rods is fixedly connected to the side of the fixed sleeve, the other ends of the two sliding rods are respectively fixedly installed with fixed blocks, the sides of the two fixed blocks are fixedly connected to the sides of the fixed bars, and the outer walls of the two sliding rods are respectively slidably installed with elastic parts, and the two ends of the two elastic parts are respectively fixedly connected to the side of the fixed sleeve and the side of the movable sleeve.

[0014] Preferably, two support bars are fixedly installed on the other side of the movable sleeve, and a connecting shaft is rotatably installed on the two support bars near the side through a bearing seat. A roller is fixedly installed on the outer wall of the connecting shaft, and a plurality of protrusions are fixedly installed on the inner wall of the concentration tank. The outer wall of the roller is rollingly connected to the outer wall of the protrusion and the inside of the concentration tank.

[0015] Preferably, a mounting bar is fixedly mounted on the bottom surface of the fixing bar, a knocking rod is hinged at the lower end of the mounting bar, the other end of the knocking rod is fixedly connected to the outer wall of the knocking head, and the outer wall of the knocking head is movably connected to the top surface of the fixed shell.

[0016] Preferably, a fixed rod is fixedly installed on the bottom surface of the movable sleeve, a circular plate is fixedly installed on the bottom surface of the fixed rod, a plurality of limit rods are fixedly installed on the top surface of the circular plate and the bottom surface of the movable sleeve, a sliding sleeve is slidably installed on the outer wall of the limit rod and the outer wall of the fixed rod, a hinge block is fixedly installed on the outer wall of the sliding sleeve, and the inner wall of the hinge block is hinged to one end of the knocking rod.

[0017] Preferably, a stirring rod is fixedly installed on the outer wall of the rotating shaft, the side of the movable sleeve is fixedly connected to the side of the fixed plate, the other end of the fixed plate slides through the side of the fixed sleeve and extends to the other side of the fixed sleeve, multiple stirring columns are provided, and the bottom surface of the fixed plate is fixedly connected to the top surface of the stirring column.

[0018] The herpes zoster vaccine production process includes the following steps:

[0019] Step 1: Virus strain selection and cultivation

[0020] S1. Use an attenuated herpes zoster virus strain that has been cultured for a long time to reduce its virulence while still retaining its immunogenicity;

[0021] S2. Inoculate the virus strain into a suitable cell line for virus amplification and culture;

[0022] Step 2: Virus ultrafiltration purification and concentration

[0023] S3. When the cytopathic effect reaches a certain level, the virus solution is harvested and added to a concentration tank;

[0024] S4, filtering through pressurization and ultrafiltration membrane while stirring the column;

[0025] S5, the knocking head makes the ultrafiltration membrane vibrate, which makes the ultrafiltration membrane less likely to be blocked and performs concentration and purification at the same time;

[0026] Step 3: Inactivation and adjuvant preparation

[0027] S6. For some live attenuated vaccines, inactivation may be necessary to ensure safety and the addition of appropriate adjuvants to enhance the immune response;

[0028] Step 4: Preparation and packaging

[0029] S7, mixing the purified virus solution with an adjuvant to prepare a vaccine preparation;

[0030] S8, perform sterile filtration and filling to finally obtain the finished vaccine.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention starts the motor to drive the rotating shaft and the fixed bar to rotate, the fixed bar drives the movable sleeve and the roller to rotate, the roller rotates and reciprocates under the joint action of the protrusion and the elastic member, the roller drives the supporting bar and the movable sleeve to reciprocate, drives the fixed rod and the sliding sleeve to reciprocate, and the sliding sleeve moves up and down on the outer wall of the fixed rod when it moves left and right. The sliding sleeve drives the knocking rod to rotate back and forth through the hinge block, and the knocking rod drives the knocking head to knock and vibrate the fixed shell back and forth, and the fixed shell drives the ultrafiltration membrane to vibrate, so that the gel layer is not easily generated on the surface of the ultrafiltration membrane, the holes of the ultrafiltration membrane are not easily clogged, and the filtration efficiency of the ultrafiltration membrane and the production efficiency are improved;

[0033] The stirring rod is provided to stir the shingles vaccine liquid inside the concentration tank more evenly. When the movable sleeve moves back and forth, it drives the fixed plate and the stirring column to move back and forth, so that the stirring column rotates and moves back and forth at the same time, stirring the shingles vaccine liquid inside the concentration tank, making the ultrafiltration membrane less likely to be clogged, thereby improving the filtration efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0035] Figure 2 This is an exploded view of the three-dimensional structure of the can cover of the present invention;

[0036] Figure 3 This is a schematic diagram of the three-dimensional structure of the stirring rod of the present invention;

[0037] Figure 4 It is a schematic cross-sectional view of the three-dimensional structure of the concentration tank of the present invention;

[0038] Figure 5 This is an exploded view of the three-dimensional structure of the ultrafiltration membrane of the present invention;

[0039] Figure 6 For the present invention Figure 4 Enlarged view of point A in the middle;

[0040] Figure 7 It is a schematic diagram of the three-dimensional structure of the roller of the present invention;

[0041] Figure 8 It is an exploded view of the three-dimensional structure of the movable sleeve of the present invention.

[0042] In the picture:

[0043] 1. Concentration tank; 101. Tank cover; 102. Connecting assembly; 103. Mounting block; 104. Drain pipe 1; 105. Drain pipe 2; 106. Water inlet pipe; 107. Booster pipe; 108. Exhaust pipe; 109. Pressure gauge; 110. Ultrafiltration membrane; 111. Fixing shell; 112. Pressure ring;

[0044] 2. Vibration mechanism; 201. Motor box; 202. Motor; 203. Rotating shaft; 204. Fixing bar; 205. Fixing sleeve; 206. Fixing block; 207. Sliding rod; 208. Moving sleeve; 209. Elastic member; 210. Support bar; 211. Connecting shaft; 212. Roller; 213. Bump; 214. Mounting bar; 215. Knocking rod; 216. Knocking head; 217. Articulated block; 218. Sliding sleeve; 219. Fixing rod; 220. Limiting rod; 221. Circular plate;

[0045] 3. Stirring mechanism; 301. Stirring rod; 302. Fixed plate; 303. Stirring column. DETAILED DESCRIPTION

[0046] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0047] In order to enable those skilled in the art to better understand the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts should fall within the scope of protection of this application.

[0048] like Figures 1-8 As shown, the present application provides an ultrafiltration purification and concentration device for a herpes zoster vaccine production system, comprising: a concentration tank 1, a tank cover 101 installed on the top surface of the concentration tank 1, a mounting block 103 fixedly installed on the outer wall of the concentration tank 1, a drain pipe 105 and a water inlet pipe 106 fixedly installed through the outer wall of the concentration tank 1, an ultrafiltration membrane 110 disposed inside the concentration tank 1, and further comprising:

[0049] The vibration mechanism 2 is disposed inside the concentration tank 1 and includes a rotating shaft 203 located inside the concentration tank 1. Two fixed sleeves 205 and two movable sleeves 208 are disposed below the rotating shaft 203. A striking head 216 is disposed below the movable sleeve 208. The vibration mechanism 2 causes the ultrafiltration membrane 110 to vibrate.

[0050] Specifically, such as Figures 1-8 As shown, the concentration tank 1 is connected to the tank cover 101 through a connecting assembly 102, a drain pipe 104 is fixedly installed on the bottom surface of the concentration tank 1, a fixed shell 111 is fixedly installed on the inner wall of the concentration tank 1, the inner wall of the fixed shell 111 is slidably connected to the outer wall of the ultrafiltration membrane 110, a pressure ring 112 is installed on the edge of the top surface of the ultrafiltration membrane 110, and the top surface of the pressure ring 112 is fixedly connected to the top surface of the fixed shell 111 by bolts.

[0051] In this embodiment, the herpes zoster vaccine is filtered by the ultrafiltration membrane 110 , and the pressure ring 112 and the fixing shell 111 are provided to fix the ultrafiltration membrane 110 , so as to facilitate the installation and fixation of the ultrafiltration membrane 110 .

[0052] Specifically, such as Figures 1-8 As shown, a pressurizing pipe 107 and an exhaust pipe 108 are fixedly installed through the top surface of the tank cover 101, and a pressure gauge 109 is fixedly installed through the top surface of the tank cover 101.

[0053] In this embodiment, the air pressure inside the concentration tank 1 is detected by the provided pressure gauge 109 , so that the air pressure inside the concentration tank 1 can be observed intuitively.

[0054] Specifically, such as Figures 1-8As shown, a motor box 201 is fixedly installed on the bottom surface of the tank cover 101 by bolts, a motor 202 is fixedly installed on the inner wall of the motor box 201, the upper end of the rotating shaft 203 rotates through the bottom surface of the motor box 201 and extends to the inside of the motor box 201, and the upper end of the rotating shaft 203 is fixedly connected to the output end of the motor 202.

[0055] In this embodiment, the motor 202 drives the rotating shaft 203 to rotate, and the motor box 201 fixes the motor 202 .

[0056] Specifically, such as Figures 1-8 As shown, a fixing bar 204 is fixedly installed at the lower end of the rotating shaft 203 , the outer wall of the fixing bar 204 is fixedly connected to the inner walls of the two fixing sleeves 205 , and the inner walls of the two movable sleeves 208 are slidably connected to the outer wall of the fixing bar 204 .

[0057] In this embodiment, the fixing bar 204 is driven to rotate by the rotating shaft 203 , and the fixing bar 204 fixes the fixing sleeve 205 .

[0058] Specifically, such as Figures 1-8 As shown, two sliding rods 207 are installed on the side of the movable sleeve 208 for sliding through, one end of the two sliding rods 207 is fixedly connected to the side of the fixed sleeve 205, and the other ends of the two sliding rods 207 are respectively fixedly installed with fixed blocks 206, and the sides of the two fixed blocks 206 are fixedly connected to the sides of the fixed bars 204, and the outer walls of the two sliding rods 207 are respectively slidably installed with elastic parts 209, and the two ends of the two elastic parts 209 are respectively fixedly connected to the sides of the fixed sleeve 205 and the side of the movable sleeve 208.

[0059] In this embodiment, the movable sleeve 208 is limited by the provided sliding rod 207 , and the provided elastic member 209 applies elastic force to the movable sleeve 208 .

[0060] Specifically, such as Figures 1-8 As shown, two support bars 210 are fixedly installed on the other side of the movable sleeve 208. The two support bars 210 are rotatably installed with a connecting shaft 211 near the side through a bearing seat. A roller 212 is fixedly installed on the outer wall of the connecting shaft 211. A plurality of protrusions 213 are fixedly installed on the inner wall of the concentration tank 1. The outer wall of the roller 212 is rollingly connected to the outer wall of the protrusion 213 and the inside of the concentration tank 1.

[0061] In this embodiment, the protrusion 213 cooperates with the elastic member 209 to enable the roller 212 to move back and forth.

[0062] Specifically, such as Figures 1-8 As shown, a mounting bar 214 is fixedly installed on the bottom surface of the fixing bar 204, and a knocking rod 215 is hinged at the lower end of the mounting bar 214. The other end of the knocking rod 215 is fixedly connected to the outer wall of the knocking head 216, and the outer wall of the knocking head 216 is movably connected to the top surface of the fixed shell 111.

[0063] In this embodiment, the knocking rod 215 is provided to drive the knocking head 216 to knock the fixed shell 111, so that the fixed shell 111 and the ultrafiltration membrane 110 vibrate.

[0064] Specifically, such as Figures 1-8 As shown, a fixed rod 219 is fixedly installed on the bottom surface of the movable sleeve 208, a circular plate 221 is fixedly installed on the bottom surface of the fixed rod 219, a plurality of limiting rods 220 are fixedly installed on the top surface of the circular plate 221 and the bottom surface of the movable sleeve 208, a sliding sleeve 218 is slidably installed on the outer wall of the limiting rod 220 and the outer wall of the fixed rod 219, a hinge block 217 is fixedly installed on the outer wall of the sliding sleeve 218, and the inner wall of the hinge block 217 is hinged to one end of the knocking rod 215.

[0065] In this embodiment, the sliding sleeve 218 is limited by the provided limiting rod 220, so that the sliding sleeve 218 and the hinge block 217 can move more stably.

[0066] The stirring mechanism 3 is provided inside the concentration tank 1 . The stirring mechanism 3 includes a fixed plate 302 and a stirring column 303 located below the rotating shaft 203 . The stirring mechanism 3 is used to stir the liquid.

[0067] Specifically, such as Figures 1-8 As shown, a stirring rod 301 is fixedly installed on the outer wall of the rotating shaft 203, the side of the movable sleeve 208 is fixedly connected to the side of the fixed plate 302, and the other end of the fixed plate 302 slides through the side of the fixed sleeve 205 and extends to the other side of the fixed sleeve 205. There are multiple stirring columns 303, and the bottom surface of the fixed plate 302 is fixedly connected to the top surface of the stirring column 303.

[0068] In this embodiment, the stirring rod 301 and the stirring column 303 are provided to stir the herpes zoster vaccine liquid in the concentration tank 1 more evenly.

[0069] The herpes zoster vaccine production process includes the following steps:

[0070] Step 1: Virus strain selection and cultivation

[0071] S1. Use an attenuated herpes zoster virus strain that has been cultured for a long time to reduce its virulence while still retaining its immunogenicity;

[0072] S2. Inoculate the virus strain into a suitable cell line for virus amplification and culture;

[0073] Step 2: Virus ultrafiltration purification and concentration

[0074] S3. When the cytopathic effect reaches a certain level, the virus solution is harvested and added to the concentration tank 1;

[0075] S4, filtering through pressurization and ultrafiltration membrane 110 while stirring column 303;

[0076] S5, the knocking head 216 causes the ultrafiltration membrane 110 to vibrate, making the ultrafiltration membrane 110 less likely to be clogged, while performing concentration and purification;

[0077] Step 3: Inactivation and adjuvant preparation

[0078] S6. For some live attenuated vaccines, inactivation may be necessary to ensure safety and the addition of appropriate adjuvants to enhance the immune response;

[0079] Step 4: Preparation and packaging

[0080] S7, mixing the purified virus solution with an adjuvant to prepare a vaccine preparation;

[0081] S8, perform sterile filtration and filling to finally obtain the finished vaccine.

[0082] The specific solution is as follows: adding shingles vaccine liquid from the water inlet pipe 106 to increase the pressure, turning on the motor 202, driving the rotating shaft 203 and the fixed bar 204 to rotate, the fixed bar 204 drives the movable sleeve 208 and the roller 212 to rotate, the roller 212 rotates and reciprocates under the joint action of the protrusion 213 and the elastic member 209, the roller 212 drives the support bar 210 and the movable sleeve 208 to move back and forth, drives the fixed rod 219 and the sliding sleeve 218 to move back and forth, and when the sliding sleeve 218 moves left and right, it moves up and down on the outer wall of the fixed rod 219, and the sliding sleeve 218 drives the knocking rod 215 to rotate back and forth through the hinge block 217, and the knocking rod 215 drives the knocking head 216 reciprocates to knock and vibrate the fixed shell 111, and the fixed shell 111 drives the ultrafiltration membrane 110 to vibrate, so that a gel layer is not easily generated on the surface of the ultrafiltration membrane 110, and the holes of the ultrafiltration membrane 110 are not easily clogged, thereby improving the filtration efficiency and production efficiency of the ultrafiltration membrane 110. At the same time, the rotating shaft 203 drives the stirring rod 301 to stir the shingles vaccine liquid inside the concentration tank 1 more evenly. When the movable sleeve 208 reciprocates, it drives the fixed plate 302 and the stirring column 303 to reciprocate, so that the stirring column 303 rotates and reciprocates at the same time, stirring the shingles vaccine liquid inside the concentration tank 1, making the ultrafiltration membrane 110 not easily clogged, thereby improving the efficiency of filtration and concentration.

[0083] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative; within the scope of the present invention, the technical features of the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0084] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. Ultrafiltration purification and concentration device for herpes zoster vaccine production system, including: A concentration tank (1), wherein a tank cover (101) is installed on the top surface of the concentration tank (1), a mounting block (103) is fixedly installed on the outer wall of the concentration tank (1), a second drain pipe (105) and a water inlet pipe (106) are fixedly installed through the outer wall of the concentration tank (1), and an ultrafiltration membrane (110) is arranged inside the concentration tank (1), characterized in that it further comprises: A vibration mechanism (2) is provided inside a concentration tank (1), the vibration mechanism (2) comprising a rotating shaft (203) located inside the concentration tank (1), two fixed sleeves (205) and two movable sleeves (208) being provided below the rotating shaft (203), a striking head (216) being provided below the movable sleeve (208), a fixed shell (111) being fixedly installed on the inner wall of the concentration tank (1), a fixed bar (204) being fixedly installed on the lower end of the rotating shaft (203), an outer wall of the fixed bar (204) being fixedly connected to the inner walls of the two fixed sleeves (205), and the inner walls of the two movable sleeves (208) being fixedly connected to the fixed bar (2 04) The outer wall is slidably connected, and two slide bars (207) are installed on the side of the movable sleeve (208) for sliding through. One end of the two slide bars (207) is fixedly connected to the side of the fixed sleeve (205), and the other end of the two slide bars (207) is fixedly installed with a fixed block (206). The side of the two fixed blocks (206) is fixedly connected to the side of the fixed bar (204). The outer walls of the two slide bars (207) are slidably installed with elastic members (209). The two ends of the two elastic members (209) are fixedly connected to the side of the fixed sleeve (205) and the side of the movable sleeve (208). The other side of the movable sleeve (208) is fixedly installed There are two support bars (210), and the two support bars (210) are rotatably mounted with a connecting shaft (211) near the side through a bearing seat, and a roller (212) is fixedly mounted on the outer wall of the connecting shaft (211), and a plurality of protrusions (213) are fixedly mounted on the inner wall of the concentration tank (1), and the outer wall of the roller (212) is in rolling connection with the outer wall of the protrusion (213) and the inside of the concentration tank (1), and a mounting bar (214) is fixedly mounted on the bottom surface of the fixing bar (204), and a knocking rod (215) is hinged at the lower end of the mounting bar (214), and the other end of the knocking rod (215) is fixedly connected to the outer wall of the knocking head (216), and the knocking head (216) is fixedly mounted on the outer wall of the knocking head (216). 16) The outer wall is movably connected to the top surface of the fixed shell (111); a fixed rod (219) is fixedly installed on the bottom surface of the movable sleeve (208); a circular plate (221) is fixedly installed on the bottom surface of the fixed rod (219); a plurality of limiting rods (220) are fixedly installed on the top surface of the circular plate (221) and the bottom surface of the movable sleeve (208); a sliding sleeve (218) is slidably installed on the outer wall of the limiting rod (220) and the outer wall of the fixed rod (219); a hinge block (217) is fixedly installed on the outer wall of the sliding sleeve (218); the inner wall of the hinge block (217) is hinged to one end of the knocking rod (215); and the vibration mechanism (2) causes the ultrafiltration membrane (110) to vibrate; A stirring mechanism (3) is provided inside the concentration tank (1), the stirring mechanism (3) comprising a fixed plate (302) and a stirring column (303) located below the rotating shaft (203), and the stirring mechanism (3) is used to stir the liquid.

2. The ultrafiltration purification and concentration device of the herpes zoster vaccine production system according to claim 1, characterized in that: The concentration tank (1) is connected to the tank cover (101) via a connecting assembly (102); a drain pipe (104) is fixedly installed on the bottom surface of the concentration tank (1); the inner wall of the fixed shell (111) is slidably connected to the outer wall of the ultrafiltration membrane (110); a pressure ring (112) is installed on the edge of the top surface of the ultrafiltration membrane (110); and the top surface of the pressure ring (112) is fixedly connected to the top surface of the fixed shell (111) via bolts.

3. The ultrafiltration purification and concentration device of the herpes zoster vaccine production system according to claim 1, characterized in that: A pressurizing pipe (107) and an exhaust pipe (108) are fixedly installed through the top surface of the tank cover (101), and a pressure gauge (109) is fixedly installed through the top surface of the tank cover (101).

4. The ultrafiltration purification and concentration device of the herpes zoster vaccine production system according to claim 1, characterized in that: The bottom surface of the tank cover (101) is fixedly mounted with a motor box (201) by means of bolts, and the inner wall of the motor box (201) is fixedly mounted with a motor (202). The upper end of the rotating shaft (203) rotates through the bottom surface of the motor box (201) and extends into the interior of the motor box (201). The upper end of the rotating shaft (203) is fixedly connected to the output end of the motor (202).

5. The ultrafiltration purification and concentration device of the herpes zoster vaccine production system according to claim 1, characterized in that: A stirring rod (301) is fixedly mounted on the outer wall of the rotating shaft (203); the side surface of the movable sleeve (208) is fixedly connected to the side surface of the fixed plate (302); the other end of the fixed plate (302) slides through the side surface of the fixed sleeve (205) and extends to the other side of the fixed sleeve (205); a plurality of stirring columns (303) are provided; and the bottom surface of the fixed plate (302) is fixedly connected to the top surface of the stirring column (303).

6. A process for producing a herpes zoster vaccine, comprising the steps of: Step 1: Virus strain selection and cultivation S1. Use an attenuated herpes zoster virus strain that has been cultured for a long time to reduce its virulence while still retaining its immunogenicity; S2. Inoculate the virus strain into a suitable cell line for virus amplification and culture; Step 2: Virus ultrafiltration purification and concentration S3. When the cytopathic effect reaches a certain level, the virus solution is harvested and added to the concentration tank (1); S4, filtering through pressurization and ultrafiltration membrane (110) while stirring the column (303); S5, the knocking head (216) causes the ultrafiltration membrane (110) to vibrate, so that the ultrafiltration membrane (110) is not easily clogged, and concentration and purification are performed at the same time; Step 3: Inactivation and adjuvant preparation S6. For some live attenuated vaccines, inactivation may be necessary to ensure safety and the addition of appropriate adjuvants to enhance the immune response; Step 4: Preparation and packaging S7, mixing the purified virus solution with an adjuvant to prepare a vaccine preparation; S8, perform sterile filtration and filling to finally obtain the finished vaccine.

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