Quantitative filling machine for culture medium

Abstract

The invention discloses a culture medium quantitative filling machine which comprises a filling machine base, a quantitative filling device, an electric guide rail and a controller, three supporting plates are fixedly connected to the upper portion of the filling machine base, through holes are formed in the centers of the supporting plates, and the quantitative filling device is fixedly connected to the side faces of the tops of the supporting plates; the quantitative filling device comprises an upper sealing plate, a quantitative liquid storage rotating disc and a lower sealing plate, a rotating shaft is fixedly connected to a through hole of the upper sealing plate, the outer surface of the rotating shaft is rotationally connected with the quantitative liquid storage rotating disc, and a liquid storage groove with the two ends communicated is formed in the quantitative liquid storage rotating disc. The lower portion of the quantitative liquid storage rotating disc is rotationally connected with a lower sealing plate, the bottom of a rotating shaft of the lower sealing plate is fixedly connected, the lower sealing plate is fixedly connected to a supporting plate, and the device solves the problems that an existing culture medium filling machine is large in quantitative error and low in filling efficiency during filling.

Description

Technical Field

[0001] The present invention relates to the technical field of culture medium filling, and specifically provides a quantitative filling machine for culture medium. Background Art

[0002] A culture medium is a nutrient matrix composed of a combination of various nutrients, usually including several major substances such as carbohydrates, nitrogen sources, minerals, vitamins, and water. These substances are essential for the growth and reproduction of microorganisms, plants, or animals (or tissues). As a basic substance providing cell nutrition and promoting cell proliferation, the culture medium provides necessary nutritional support for the growth of organisms. As an indispensable device in laboratory and industrial production, the current culture medium quantitative filling machines on the market use flow meters for quantitative filling. However, limited by the performance of the flow meters, there are measurement deviations. When performing small-dose filling, due to the action of pressure, a part of the culture medium will remain at the nozzle, affecting the next filling. Therefore, there is a need to provide a culture medium quantitative filling machine to solve the problems of large filling errors and residual culture medium at the nozzle. Summary of the Invention

[0003] The purpose of the present invention is to provide a quantitative filling machine for culture medium to solve the problems raised in the above background art.

[0004] To solve the above technical problems, the present invention provides the following technical solutions:

[0005] In the first aspect of the present invention, there is provided a quantitative filling machine for culture medium, including a filling machine base,

[0006] Above the filling machine base, two first support plates and a second support plate are fixedly connected. T-shaped through holes penetrating through are respectively provided inside the first support plate and the second support plate;

[0007] The first quantitative filling device includes:

[0008] A first screw rod penetrating through the T-shaped through hole of the first support plate, which is rotatably connected to the first support plate;

[0009] A first quantitative liquid storage tray threadedly engaged with the first screw rod, with threads on its outer surface and a plurality of first liquid storage grooves penetrating through both ends inside;

[0010] An upper sealing plate and a lower sealing plate fixed to the first support plate are respectively provided with a first liquid inlet hole and a first liquid outlet hole communicating with the first liquid storage groove;

[0011] A first rotating shaft connecting the upper and lower sealing plates drives the first quantitative liquid storage tray to rotate to adjust the coincidence state of the liquid storage groove with the liquid inlet hole and the liquid outlet hole;

[0012] The second quantitative filling device includes:

[0013] The second screw rod passing through the T-shaped through hole of the second support plate is rotatably connected to the second support plate;

[0014] The second quantitative liquid storage tray threadedly engaged with the second screw rod has a plurality of second liquid storage grooves and a first air passage penetrating the liquid storage grooves formed inside;

[0015] The upper sealing plate two and the lower sealing plate two fixed to the second support plate are respectively provided with a second liquid inlet hole and a second liquid outlet hole;

[0016] The rotating shaft two connects the upper and lower sealing plates two, and a third air passage communicating the first air passage with the second air passage of the upper sealing plate two is provided inside;

[0017] The culture medium supply system includes:

[0018] The culture medium box is respectively connected to the first liquid inlet hole and the second liquid inlet hole through a funnel one and a funnel two;

[0019] The hydraulic press drives the culture medium in the culture medium box to flow under pressure;

[0020] The electric guide rail drives the sliding platform provided with a motor to move the culture medium container;

[0021] The transmission system includes:

[0022] The first bevel gear at the bottom of the first screw rod is meshed with the second bevel gear of the transmission rod one;

[0023] The third bevel gear at the bottom of the second screw rod is meshed with the fourth bevel gear of the transmission rod two;

[0024] The motor is coupled and driven with the transmission rod one and the transmission rod two through a semi-cylindrical body;

[0025] The controller controls the coordinated actions of the hydraulic press, the electric guide rail and the motor.

[0026] The present invention further illustrates that the liquid storage groove volumes of the first quantitative liquid storage tray and the second quantitative liquid storage tray are configured in a three-level gradient of 10 mL, 1 mL and 0.1 mL respectively, and a mass sensor is built into the sliding platform, and the filling operations of the corresponding liquid storage trays are triggered in stages through the controller.

[0027] The present invention further illustrates that the air passage system of the second quantitative filling device includes:

[0028] The first air passage at the bottom of the second liquid storage groove leads to the center of the rotating shaft two;

[0029] The second air passage on the inner wall of the through hole of the upper sealing plate two communicates the outside with the third air passage of the rotating shaft two;

[0030] When the second liquid storage tank is aligned with the second liquid outlet hole, the first air passage is communicated with the third air passage, and the hydraulic press pressurizes to drive the culture medium to be completely discharged.

[0031] The present invention further illustrates that semi-cylindrical bodies are provided at the docking ends of the motor main shaft with the first transmission rod and the second transmission rod, and a complete cylindrical transmission structure is formed after combination.

[0032] The present invention further illustrates that the upper sealing plate and the lower sealing plate achieve dynamic sealing with the first quantitative liquid storage tray through the first rotating shaft, and the phase difference between the liquid inlet hole and the liquid outlet hole is 180 degrees.

[0033] The present invention further illustrates that three groups of outlets are provided at the bottom of the culture medium box, two of which are connected to the first quantitative filling device, and the last one is connected to the second quantitative filling device through the second funnel.

[0034] The present invention further illustrates that the controller controls the start and stop of the motor in sections according to the feedback signal of the quality sensor to achieve precise filling of the tens, units, and the first digit after the decimal point.

[0035] The present invention further illustrates that the movable rod of the hydraulic press is rigidly connected to the lid of the culture medium box, and when pressurized, the lid presses down to increase the hydraulic pressure inside the box.

[0036] The present invention further illustrates that the movable rod of the hydraulic press is rigidly connected to the lid of the culture medium box, and when pressurized, the lid presses down to increase the hydraulic pressure inside the box.

[0037] The present invention further illustrates that a motor perpendicular to the direction of the guide rail is provided at the bottom of the sliding platform of the electric guide rail, and the motor automatically switches the transmission coupling object when moving with the platform to different filling stations.

[0038] The present invention further illustrates that the rotation angles of the first quantitative liquid storage tray and the second quantitative liquid storage tray are determined by the lead of the screw thread and the number of liquid storage tanks, and a single rotation triggers the liquid inlet-discharge cycle of a single liquid storage tank. Compared with the prior art, the beneficial effects achieved by the present invention are as follows: In the present invention,

[0039] Through the design of the quantitative liquid storage tray, the present invention realizes the stepped quantitative filling of the culture medium. The first liquid storage tank and the second liquid storage tank with different volumes respectively correspond to the filling requirements of different digits. Combined with the real-time monitoring of the quality sensor, the precise control of the filling volume is ensured, and the problem of large quantitative errors of traditional filling machines is effectively solved.

[0040] Aiming at the problems of unsmooth outflow or residue of the culture medium that may occur in the small-aperture quantitative liquid storage tray, the present invention cleverly utilizes the principle of pressure difference and air circulation by introducing the design of the hydraulic press and the air passage, ensuring the smooth outflow and complete emptying of the culture medium, and avoiding the occurrence of residue and blockage phenomena. Description of the Drawings

[0041] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention. In the accompanying drawings:

[0042] Figure 1 is a schematic front view of the overall structure of an embodiment of the present invention;

[0043] Figure 2 is a schematic sectional view of a component structure of a quantitative liquid storage tray of an embodiment of the present invention;

[0044] Figure 3 is a schematic enlarged partial front view of the overall structure of an embodiment of the present invention;

[0045] Figure 4 is a schematic enlarged sectional view of a partial component of a drive rod of an embodiment of the present invention;

[0046] Figure 5 is a schematic rear view of the overall structure of an embodiment of the present invention;

[0047] Figure 6 is a schematic sectional view of a component structure of a second quantitative liquid storage tray of an embodiment of the present invention;

[0048] Figure 7 is a schematic enlarged sectional view of a partial component of a second drive rod of an embodiment of the present invention;

[0049] Figure 8 is a schematic view of the structure of a culture medium box assembly of an embodiment of the present invention;

[0050] Figure 9 is a schematic internal sectional view of the second quantitative liquid storage tray of an embodiment of the present invention;

[0051] In the figure: 1. Base of the filling machine; 2. First support plate; 3. First upper sealing plate; 301. First liquid inlet hole; 4. First rotating shaft; 5. First quantitative liquid storage tray; 51. First liquid storage tank; 6. First lower sealing plate; 61. First liquid outlet hole; 7. First nozzle; 8. First helical gear; 9. Second helical gear; 10. First transmission rod; 11. First bearing seat; 12. Culture medium tank; 13. Tank cover; 14. Hydraulic press; 15. Hydraulic press mounting plate; 16. First funnel; 17. Electric guide rail; 18. Sliding platform; 19. Motor; 20. Controller; 21. First screw; 22. Sealing plug; 23. Support column; 24. Second support plate; 25. Second screw; 26. Second quantitative liquid storage tray; 261. Second liquid storage tank; 262. First air passage; 27. Second rotating shaft; 271. Third air passage; 28. Second upper sealing plate; 281. Second liquid inlet hole; 282. Second air passage; 29. Second lower sealing plate; 291. Second liquid outlet hole; 30. Second nozzle; 31. Third helical gear; 32. Fourth helical gear; 33. Second moving rod; 34. Second bearing seat; 35. Second funnel. Detailed implementation mode

[0052] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0053] Please refer to Figure 1 — Figure 9 , the present invention provides a technical solution: a culture medium quantitative filling machine, including a base 1 of the filling machine, a quantitative filling device, and an electric guide rail 17. Two first support plates 2 are fixedly connected above the base 1 of the filling machine. A T-shaped through hole is opened inside the first support plate 2. A first screw 21 is arranged in the through hole of the first support plate 2. The first screw 21 is rotatably connected to the first support plate 2. The top of the first screw 21 is meshed with a first quantitative liquid storage tray 5. The outer surface of the first quantitative liquid storage tray 5 has threads. A first liquid storage tank 51 with two ends communicating is opened inside the first quantitative liquid storage tray 5. The first liquid storage tank 51 is used to store the culture medium. A through hole is opened in the center of the first quantitative liquid storage tray 5. A first rotating shaft 4 is rotatably connected in the through hole of the first quantitative liquid storage tray 5.

[0054] One end of the first rotating shaft 4 is fixedly connected to a first upper sealing plate 3. The first upper sealing plate 3 is used to seal the first liquid storage tank 51. A first liquid inlet hole 301 is opened inside the first upper sealing plate 3. The first liquid inlet hole 301 is used to guide the culture medium into the first liquid storage tank 51. A through hole is opened in the center of the first upper sealing plate 3. The through hole of the first upper sealing plate 3 is used to connect the first rotating shaft 4. The first upper sealing plate 3 is fixedly connected to the first support plate 2.

[0055] One end of the first rotating shaft 4 is fixedly connected with a first lower sealing plate 6. The first lower sealing plate 6 is used to seal the first liquid storage tank 51. A first liquid outlet hole 61 is formed inside the first lower sealing plate 6. The first liquid outlet hole 61 is used to guide the culture medium to flow out from the first liquid storage tank 51. The first lower sealing plate 6 is fixedly connected with the first support plate 2. A first nozzle 7 is fixedly connected at the first liquid outlet hole 61. The first nozzle 7 is used to guide the culture medium to flow into the culture medium container.

[0056] As Figure 4 shown, the lower end of the first screw 21 is fixedly connected with a first helical gear 8. The first helical gear 8 is meshed with a second helical gear 9. The second helical gear 9 is fixedly connected with a first transmission rod 10. The top of the first transmission rod 10 has a semi-cylindrical body. The outer surface of the first transmission rod 10 is rotatably connected with a first bearing seat 11. The first bearing seat 11 is fixedly connected above the filling machine base 1.

[0057] As Figure 7 shown, a second support plate 24 is fixedly connected above the filling machine base 1. A T-shaped through hole is formed inside the second support plate 24. A second screw 25 is arranged in the through hole of the second support plate 24. The second screw 25 is rotatably connected with the second support plate 24. The top of the second screw 25 is meshed with a second quantitative liquid storage tray 26.

[0058] As Figure 6 and Figure 9 shown, a second liquid storage tank 261 with two ends communicating is formed inside the second quantitative liquid storage tray 26. The second liquid storage tank 261 is used to store the culture medium. A first air channel 262 is formed at the bottom of the second liquid storage tank 261. The first air channel 262 leads to the center of the second quantitative liquid storage tray 26. A through hole is formed in the center of the second quantitative liquid storage tray 26. A second rotating shaft 27 is rotatably connected in the through hole of the second quantitative liquid storage tray 26.

[0059] One end of the second rotating shaft 27 is fixedly connected with a second upper sealing plate 28. The second upper sealing plate 28 is used to seal the second liquid storage tank 261. A second liquid inlet hole 281 is formed inside the second upper sealing plate 28. The second liquid inlet hole 281 is used to guide the culture medium to enter the second liquid storage tank 261. A through hole is formed in the center of the second upper sealing plate 28. A second air channel 282 is formed on the inner wall of the through hole of the second upper sealing plate 28. The second air channel 282 connects the through hole with the bottom surface of the second upper sealing plate 28. The through hole of the second upper sealing plate 28 is used to connect the second rotating shaft 27.

[0060] On the horizontal plane of the first air channel 262 and the second air channel 282, a third air channel 271 is formed on the second rotating shaft 27. The third air channel 271 communicates at the center line of the second rotating shaft 27.

[0061] The other end of the second rotating shaft 27 is fixedly connected with a second lower sealing plate 29. The second lower sealing plate 29 is used to seal the second liquid storage tank 261. A second liquid outlet hole 291 is formed inside the second lower sealing plate 29. The second liquid outlet hole 291 is used to guide the culture medium to flow out of the second liquid storage tank 261. A second nozzle 30 is fixedly connected at the second liquid outlet hole 291. The second nozzle 30 is used to guide the culture medium to flow into the culture medium container.

[0062] As Figure 5 shown, the second upper sealing plate 28 and the second lower sealing plate 29 are fixedly connected to the second support plate 24.

[0063] As Figure 7 shown, the lower end of the second screw 25 is fixedly connected with a third helical gear 31. The third helical gear 31 is meshed and connected with a fourth helical gear 32. The fourth helical gear 32 is fixedly connected with a second transmission rod 33. The top of the second transmission rod 33 has a semi-cylindrical body. A second bearing seat 34 is rotatably connected to the outer surface of the second transmission rod 33. The second bearing seat 34 is fixedly connected above the filling machine base 1.

[0064] As Figure 5 and Figure 8 shown, a support column 23 is fixedly connected above the filling machine base 1. The top of the support column 23 is fixedly connected with a culture medium tank 12. A tank cover 13 is arranged inside the culture medium tank 12. A liquid adding hole is formed in the tank cover 13. A sealing plug 22 is arranged at the liquid adding hole of the tank cover 13. The sealing plug 22 is used to seal the culture medium tank 12.

[0065] Above the tank cover 13 is fixedly connected with a hydraulic press 14. The hydraulic press 14 is fixedly connected with a hydraulic press mounting plate 15. The hydraulic press mounting plate 15 is fixedly connected above the culture medium tank 12. The movable rod of the hydraulic press 14 is connected with the tank cover 13. The fixed rod of the hydraulic press 14 is fixedly connected with the hydraulic press mounting plate 15. Three through holes are formed at the bottom of the culture medium tank 12.

[0066] As Figure 2 shown, at the first two through holes of the culture medium tank 12 is fixedly connected with a first funnel 16. The bottom of the first funnel 16 is fixedly connected with the first liquid inlet hole 301.

[0067] As Figure 6 shown, at the last through hole of the culture medium tank 12 is fixedly connected with a second funnel 35. The bottom of the second funnel 35 is fixedly connected with the second liquid inlet hole 281.

[0068] As Figure 1As shown in the figure, a sliding platform 18 is arranged around the electric guide rail 17. The sliding platform 18 encloses the electric guide rail 17. A motor 19 is fixedly connected to the bottom of the sliding platform 18 to meet the requirement that the motor 19 slides below the electric guide rail 17 following the sliding platform 18. The top of the main shaft of the motor 19 has a semi-cylindrical body. The semi-cylindrical body of the main shaft of the motor 19 is used to combine with the semi-cylindrical bodies of the first transmission rod 10 and the second transmission rod 33 into a cylindrical body to meet the requirement that the main shaft of the motor 19 drives the first transmission rod 10 and the second transmission rod 33 to rotate.

[0069] A controller 20 is fixedly connected to the side of the electric guide rail 17. The hydraulic press 14, the electric guide rail 17 and the motor 19 are controlled by the controller 20.

[0070] Embodiment 1: In this embodiment, to realize the operation of the culture medium filling machine, the filling work is carried out through the filling device and the electric guide rail 17 to realize automatic filling.

[0071] Specifically, place the culture medium container to be filled on the sliding platform 18. Start the electric guide rail 17 through the controller 20. The electric guide rail 17 drives the sliding platform 18 and the culture medium container to move towards the first nozzle 7. When the culture medium container moves directly below the first nozzle 7, the electric guide rail 17 stops. At this time, the semi-cylindrical body at the top of the rotating shaft of the motor 19 combines with the semi-cylindrical body at the top of the first transmission rod 10 into a circular cylindrical body. The controller 20 controls the motor 19 to start. The rotating shaft of the motor 19 drives the first transmission rod 10 and the second helical gear 9 to rotate. The second helical gear 9 drives the first helical gear 8 and the first screw 21 to rotate. The thread on the first screw 21 drives the first quantitative liquid storage disc 5 to rotate through the meshing connection with the thread on the outer surface of the first quantitative liquid storage disc 5.

[0072] When there is an overlapping part between the first liquid storage tank 51 and the first liquid inlet hole 301, the culture medium in the culture medium tank 12 enters the first liquid storage tank 51 through the first funnel 16 and the first liquid inlet hole 301.

[0073] When there is an overlapping part between the first liquid storage tank 51 and the first liquid outlet hole 61, the culture medium in the first liquid storage tank 51 enters the culture medium container through the first liquid outlet hole 61 and the first nozzle 7.

[0074] The motor 19 stops. The controller 20 controls the electric guide rail 17 to start, and the automatic filling is completed.

[0075] Through the above steps, the filling work of the quantitative filling machine can be realized, and the filling efficiency is improved through the electric guide rail 17 device.

[0076] Embodiment 2: Based on Embodiment 1, the culture medium filling machine also has the function of quantitative filling. The first liquid storage tank 51 in the first quantitative liquid storage disc 5 is divided into different volumes step by step to solve the problem of large quantitative error during filling of the traditional culture medium filling machine.

[0077] Specifically, each first liquid storage tank 51 of the first quantitative liquid storage tray 5 can store 10 mL of culture medium, each first liquid storage tank 51 of the second quantitative liquid storage tray 5 can store 1 mL of culture medium, each second liquid storage tank 261 of the quantitative liquid storage tray two 26 can store 0.1 mL of culture medium, and a mass sensor is arranged inside the sliding platform 18.

[0078] Let the volume of the culture medium to be filled be a mL, the value of the tens digit of a be x, the value of the units digit of a be y, and the value of the first digit after the decimal point of a be z.

[0079] When the culture medium container reaches below the first nozzle 7, the electric guide rail 17 stops, and the controller 20 controls the motor 19 to start. As the first quantitative liquid storage tray 5 rotates, the culture medium continuously flows out of the nozzle 7, and the mass sensed by the mass sensor continuously increases. When the value of the tens digit of the volume of the culture medium in the culture medium container reaches x, the motor 19 stops, and the controller 20 controls the electric guide rail 17 to start.

[0080] When the culture medium container reaches below the second nozzle 7, the controller 20 controls the motor 19 to start. As the first quantitative liquid storage tray 5 rotates, the culture medium flows out of the nozzle 7. When the value of the units digit of the volume of the culture medium in the culture medium container reaches y, the motor 19 stops, and the controller 20 controls the electric guide rail 17 to start.

[0081] When the culture medium container reaches below the nozzle two 30, the controller 20 controls the motor 19 to start. As the second quantitative liquid storage tray 26 rotates, the culture medium flows out of the nozzle two 30. When the value of the first digit after the decimal point of the volume of the culture medium in the culture medium container reaches z, the motor 19 stops, and the controller 20 controls the electric guide rail 17 to start. The quantitative filling of the culture medium is completed.

[0082] Embodiment 3: Based on Embodiment 1, when the culture medium container reaches directly below the nozzle two 30, there is a hydraulic press 14 and an air passage, which solves the problem that the second liquid storage tank 261 in the second quantitative liquid storage tray 26 cannot flow out or a part remains at the nozzle two 30 due to the too small diameter of the hole under the action of air pressure.

[0083] When the second liquid storage tank 261 and the second liquid inlet hole 281 overlap, at this time, the second liquid outlet hole 291 partially overlaps with the second liquid storage tank 261 that has stored the culture medium. The first air passage 262 communicates with the third air passage 271, and the third air passage 271 communicates with the second air passage 282. At the same time, the hydraulic press 14 is started, and the movable rod of the hydraulic press 14 drives the box cover 13 to press downward. The culture medium inside the culture medium box 12 leads to the second liquid storage tank 261 through the funnel two 35 and the second liquid inlet hole 281 under the action of pressure. The air in the second liquid storage tank 261 leads to the second liquid storage tank 261 that has an overlapping part with the second liquid outlet hole 291 in the first air passage 262. The culture medium in the second liquid storage tank 261 that has an overlapping part with the lower sealing plate two 29 flows out from the second liquid outlet hole 291 under the action of the air in the first air passage 262. When all the culture medium in the second liquid storage tank 261 has flowed out, the hydraulic press 14 stops. At this time, the second liquid storage tank 261 that has an overlapping part with the second liquid inlet hole 281 is just filled with the culture medium. The filling of the culture medium container below the nozzle two 30 is completed.

[0084] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0085] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A culture medium quantitative filling machine, comprising a filling machine base, characterized in that: A filling machine base, on which two first support plates and a second support plate are fixedly connected, wherein the first support plate and the second support plate are respectively provided with a penetrating T-shaped through hole; The first quantitative filling device comprises: A first screw rod passing through the T-shaped through hole of the first support plate and rotatably connected to the first support plate; A first quantitative liquid storage disk is threadably engaged with the first screw rod, and has a threaded outer surface and a plurality of first liquid storage tanks with two ends extending therethrough; An upper sealing plate and a lower sealing plate fixed to the first supporting plate are respectively provided with a first liquid inlet hole and a first liquid outlet hole communicated with the first liquid storage tank; A rotating shaft 1 connected to the upper and lower sealing plates drives the first quantitative liquid storage disk to rotate to adjust the overlap state of the liquid storage tank with the liquid inlet hole and the liquid outlet hole; The second quantitative filling device comprises: A second screw rod passing through the T-shaped through hole of the second support plate and rotatably connected to the second support plate; A second quantitative liquid storage disk threadably meshed with the second screw rod has a plurality of second liquid storage tanks and a first air passage penetrating the liquid storage tanks disposed therein; The upper sealing plate 2 and the lower sealing plate 2 fixed to the second supporting plate are respectively provided with a second liquid inlet hole and a second liquid outlet hole; The second rotating shaft is connected to the upper and lower sealing plates 2, and a third air passage is provided inside the second rotating shaft, which is connected to the first air passage and the second air passage of the upper sealing plate 2; Culture medium supply system, including: The culture medium box is connected to the first liquid inlet and the second liquid inlet through the first funnel and the second funnel respectively; A hydraulic press drives the culture medium in the culture medium box to flow under pressure; The electric guide rail drives the sliding platform equipped with a motor to move the culture medium container; Transmission system, including: The first bevel gear at the bottom of the first screw is meshed with the second bevel gear of the transmission rod; The third bevel gear at the bottom of the second screw rod is meshed with the fourth bevel gear of the second transmission rod; The motor is coupled with the first transmission rod and the second transmission rod through the semicircular cylinder; The controller controls the coordinated actions of the hydraulic press, electric guide rails and motors.

2. A medium quantitative filling machine according to claim 1, characterized in that: The liquid storage tank volumes of the first quantitative liquid storage tray and the second quantitative liquid storage tray are respectively configured in a three-level gradient of 10 mL, 1 mL and 0.1 mL, and the sliding platform has a built-in mass sensor, which triggers the filling operation of the corresponding liquid storage tray in stages through the controller.

3. A medium quantitative filling machine according to claim 1, characterized in that: The air channel system of the second quantitative filling device comprises: A first air passage at the bottom of the second liquid storage tank leads to the center of the second rotating shaft; The second air passage on the inner wall of the second through hole of the upper sealing plate is connected to the outside and the third air passage of the second rotating shaft; When the second liquid storage tank is aligned with the second liquid outlet, the first air channel is connected to the third air channel, and the hydraulic press pressurizes and drives the culture medium to be completely discharged.

4. A medium quantitative filling machine according to claim 1, characterized in that: The butt ends of the motor main shaft and the first and second transmission rods are all provided with semicircular cylinders, which form a complete cylindrical transmission structure after being combined.

5. A medium quantitative filling machine according to claim 4, characterized in that: The upper sealing plate and the lower sealing plate realize dynamic sealing with the first quantitative liquid storage disk through the rotating shaft 1, and the phase difference between the liquid inlet and the liquid outlet is 180 degrees.

6. The quantitative filling machine for culture medium according to claim 1, wherein: The bottom of the culture medium box is provided with three groups of outlets, two of which are connected to the first quantitative filling device, and the last group is connected to the second quantitative filling device through funnel two.

7. A culture medium quantitative filling machine according to claim 1, characterized in that: The controller controls the start and stop of the motor in sections according to the feedback signal of the quality sensor to achieve precise filling of the tens, units, and the first digit after the decimal point.

8. A medium quantitative filling machine according to claim 1, characterized in that: The movable rod of the hydraulic press is rigidly connected to the lid of the culture medium tank, and when pressurized, the lid presses down to increase the hydraulic pressure inside the tank.

9. The culture medium quantitative filling machine according to claim 1, characterized in that: A motor perpendicular to the direction of the guide rail is provided at the bottom of the sliding platform of the electric guide rail, and the motor automatically switches the transmission coupling object when the platform moves to different filling stations.

10. The culture medium quantitative filling machine according to claim 1, wherein: The rotation angles of the first quantitative liquid storage tray and the second quantitative liquid storage tray are determined by the screw thread lead and the number of liquid storage grooves, and a single rotation triggers the liquid inlet-discharge cycle of a single liquid storage groove.

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