Piece dropping device for automatically measuring cell concentration and use method thereof

Abstract

The invention discloses a dropping device for automatically measuring cell concentration and a use method thereof, the dropping device comprises a rack, the rack is sequentially provided with a dilution adjusting station, a turbidity detection station, a pipetting station and a dropping station according to an operation sequence, and the rack is provided with a carrying conveying line used for directionally conveying a centrifugal tube along each station; the dilution adjusting station is provided with a dilution adjusting mechanism, the dilution adjusting mechanism comprises a diluent dropping control module and a cell suspension conveying module, the turbidity detection station is provided with a turbidity detection mechanism, the cell suspension conveying module is communicated with the turbidity detection mechanism, the pipetting station is provided with a pipetting mechanism, and the pipetting mechanism is communicated with the cell suspension conveying module. The pipetting mechanism comprises a three-dimensional moving module and a pipettor, a glass slide table is arranged on the dropping station, the glass slide table is used for bearing a glass slide, an identifier scanning device is arranged beside the pipettor, a first visual identifier is pasted on the glass slide, and a second visual identifier is pasted on the centrifugal tube. The experiment efficiency and accuracy can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical engineering, and specifically relates to a dropper device for automatically measuring cell concentration and a using method thereof. Background Art

[0002] Traditional cell suspension processing is that technicians adjust the cell concentration according to experience, prepare glass slides in advance, and after adjusting the concentration, technicians drop the cell suspension on the glass slide according to experience. The traditional processing method highly depends on the experience of the operator. An experienced operator can achieve good results in adjusting cell concentration and dropping the slide. However, the operations of adjusting cell concentration and dropping the slide have a very great impact on the experimental results of the entire fluorescence in situ hybridization technology.

[0003] First, from the analysis of cell concentration, if the density is too low, few cells are obtained on the glass slide, which increases the time for subsequent result interpretation, and because the number of cells is too small and the analyzed cells are insufficient, it is easy to lead to false negative results; if the density is too high, there are too many cells on the glass slide, which is easy to cause cell overlap. When interpreting the results, it is not easy to distinguish the cells where the results are located, which is easy to lead to false positive results. Both too high and too low cell concentrations reduce the accuracy of result interpretation.

[0004] Second, from the analysis of the dropping slide operation, the differences in manual operations are very large, which are mainly described as follows: during the dropping slide process, the height of the cell suspension from the glass slide, the dropping method, the number of drops, the position on the glass slide where the drops are made, etc. If the dropping is improper, the cells overlap, the density is uneven, the cell membranes are of different thicknesses, the cells cannot unfold normally, the cells are squeezed against each other, which increases the difficulty of the reagent entering the cells, affects the intensity of the fluorescence signal, and reduces the sensitivity and accuracy of result interpretation.

[0005] Currently, in order to achieve precise processing of cell suspensions, multiple independent devices are usually used to separately complete concentration detection, dilution adjustment, and dropping slide operations. For example, some devices focus on concentration detection and measure the concentration of cell suspensions by optical methods; some other devices focus on dilution adjustment and adjust the cell suspension to the required concentration manually or semi - automatically; there are also some devices only used for dropping slide operations to evenly distribute the cell suspension on the glass slide. In addition, some devices have tried to combine two of these functions, but still cannot achieve integrated operation of the entire process.

[0006] The current technical means have obvious defects. Due to the lack of integration between steps, the experimental process is frequently interrupted, the operation is cumbersome and time-consuming. Especially when concentration detection, dilution adjustment and dropwise operation need to be carried out simultaneously, the switching between different devices will increase the operation complexity, reduce the experimental efficiency, and may introduce human errors, affecting the reliability of experimental results. Therefore, there is an urgent need to design an integrated device that can integrate concentration detection, closed-loop dilution adjustment and precise dropwise operation. Summary of the Invention

[0007] The object of the present invention is to overcome the above-mentioned shortcomings of the prior art, and provide an integrated device for automatically measuring cell concentration and its use method, which integrates concentration detection, closed-loop dilution adjustment, and precise dropwise operation, and improves the experimental efficiency and standardization level.

[0008] The present invention is realized through the following technical solutions: A device for automatically measuring cell concentration and dropping tablets, including a frame. Along the operation sequence, a dilution adjustment station, a turbidity detection station, a pipetting station, and a dropping tablet station are sequentially arranged on the frame. A carrier conveyor line is arranged on the frame for directionally conveying the centrifuge tube along each station.

[0009] A dilution adjustment mechanism is arranged at the dilution adjustment station. The dilution adjustment mechanism includes a dilution liquid dropping control module and a cell suspension conveying module. The dilution liquid dropping control module is connected to a horizontal adjustment module, and the cell suspension conveying module is connected to a horizontal and vertical movement module.

[0010] A turbidity detection mechanism is arranged at the turbidity detection station, and the cell suspension conveying module is communicated with the turbidity detection mechanism.

[0011] A pipetting mechanism is arranged at the pipetting station. The pipetting mechanism includes a three-dimensional movement module and a pipette. The three-dimensional movement module is arranged on the frame, and the pipette is arranged on the three-dimensional movement module.

[0012] A glass slide stage is arranged at the dropping tablet station, and the glass slide stage is used for carrying glass slides.

[0013] An identification scanning device is arranged beside the pipette. A first visual identification is pasted on the glass slide, and a second visual identification is pasted on the centrifuge tube. The first visual identification and the second visual identification correspond one by one.

[0014] Furthermore: The dilution liquid dropping control module includes a liquid storage tank, a dropping tube, a flow control component, and a flow sensor. The liquid storage tank is arranged on the horizontal adjustment module. The liquid storage tank is used for storing dilution liquid. The dropping tube is communicated with the bottom of the liquid storage tank. The flow control component and the flow sensor are respectively arranged on the dropping tube, and the flow sensor is located behind the flow control component.

[0015] Further: The turbidity detection mechanism includes a colorimetric cell and a turbidimeter. The turbidimeter is fixed on the frame, the colorimetric cell is arranged in the detection area of the turbidimeter, and the colorimetric cell is made of a transparent material.

[0016] Further: The cell suspension conveying module includes a movable pipe seat, a sampling pipe, a sample conveying pipe, a liquid discharge pipe, and a discharge pipe. The movable pipe seat is connected to the horizontal and vertical movement module. The sample conveying pipe and the discharge pipe are both flexible hoses. The sampling pipe and the liquid discharge pipe are arranged in parallel at the bottom of the movable pipe seat, and the length of the sampling pipe is greater than that of the liquid discharge pipe. One end of the sample conveying pipe penetrates into the movable pipe seat and is communicated with the sampling pipe, the other end of the sample conveying pipe is communicated with the first interface of the colorimetric cell, one end of the discharge pipe penetrates into the movable pipe seat and is communicated with the liquid discharge pipe, the other end of the discharge pipe is communicated with the second interface of the colorimetric cell, and a driving source is arranged on the sample conveying pipe.

[0017] Further: It further includes a mixing and shunting pipe. One end of the mixing and shunting pipe is communicated with the part of the sample conveying pipe between the turbidimeter and the driving source, and the other end of the mixing and shunting pipe is communicated with the part of the discharge pipe close to the movable pipe seat. A first shut-off valve is arranged on the mixing and shunting pipe, and the first shut-off valve is used to control the on-off between the mixing and shunting pipe and the sample conveying pipe. A second shut-off valve is arranged on the sample conveying pipe, and the second shut-off valve is used to control the on-off between the sample conveying pipe and the colorimetric cell. A one-way valve is arranged on the discharge pipe, and the one-way valve is close to the connection part of the discharge pipe and the mixing and shunting pipe. The one-way valve is used to prevent the liquid in the mixing and shunting pipe from flowing into the colorimetric cell.

[0018] Further: The glass slide stage includes a stage body and a cover plate arranged on the stage body. A plurality of placing grooves are opened on the stage body, and a plurality of liquid dripping ports are opened on the cover plate. The liquid dripping ports and the placing grooves are arranged in one-to-one correspondence. A material taking groove is arranged at the notch of the placing groove, and a guiding sliding groove is arranged above the material taking groove.

[0019] Further: The carrier conveying line includes a carrier frame and a frame conveyor line. The carrier frame is movably arranged on the frame conveyor line. The carrier frame includes an upper support plate and a lower support plate. A support plate is connected between the upper support plate and the lower support plate to form an I-shaped structure. A plurality of positioning grooves are opened on the upper support plate, and a plurality of alignment grooves are opened on the lower support plate. The positioning grooves and the alignment grooves are arranged in one-to-one correspondence.

[0020] Further: It further includes a cleaning component. The cleaning component includes a cleaning liquid tank and a waste liquid storage tank. A cleaning inlet is opened at the top of the cleaning liquid tank, and a sewage discharge inlet is opened at the top of the waste liquid storage tank.

[0021] According to the usage method of a dropper device for automatically measuring cell concentration described above, it includes the following steps:

[0022] S1. Insert the centrifuge tube filled with cell suspension into the positioning groove of the carrier rack, place the glass slide in the placement groove of the glass slide table. At the same time, paste the first visual identifier on the glass slide and the second visual identifier on the centrifuge tube.

[0023] S2. Drive the carrier rack to move along the rack conveyor line and move the carrier rack to the dilution adjustment station.

[0024] S3. The horizontal and vertical movement module drives the movable tube seat to move forward and backward and descend, so that the sampling tube extends below the liquid level in the centrifuge tube. Turn on the drive source to extract the cell suspension in the centrifuge tube, and make the cell suspension be transported to the colorimetric cuvette through the sample delivery tube.

[0025] S4. The turbidimeter measures the transmittance and calculates the turbidity value. If it is detected that the turbidity of the cell suspension is less than or equal to the preset value, the sample liquid turbidity meets the standard, and there is no need to dilute the cell suspension, and it can directly enter the pipetting station. If it is detected that the turbidity of the cell suspension is greater than the preset value, then step S5 is executed.

[0026] S5. Turn on the flow control part, automatically calculate the required amount of diluent according to the turbidity difference, and monitor the flow rate in real time through the flow sensor to form a closed-loop control. The horizontal adjustment module drives the liquid storage tank to move forward and backward, so that the dropper tube is aligned with the corresponding centrifuge tube, and the diluent in the liquid storage tank is dropped into the corresponding centrifuge tube through the dropper tube. Then, turn on the first shut-off valve, and at the same time, close the second shut-off valve. Under the action of the drive source, the liquid in the centrifuge tube is extracted to the sample delivery tube through the sampling tube, and then flows back to the centrifuge tube through the mixing and shunting tube, the discharge tube and the drain tube. Repeat this three times to mix the diluent and the cell suspension.

[0027] S6. Extract the mixed sample liquid of the diluent and the cell suspension for turbidity retest until the turbidity meets the standard and enter the pipetting station.

[0028] S7. After the qualified cell suspension is transported to the pipetting station, the three-dimensional movement module drives the pipette to aspirate the sample liquid in the centrifuge tube according to the set amount. At the same time, the identification scanning device scans the first visual identifier on the corresponding centrifuge tube and the second visual identifier on the glass slide on the glass slide table. The three-dimensional movement module drives the pipette to move to the target dropping position, and the pipette drops the sample liquid along the corresponding dropping port onto the corresponding glass slide.

[0029] Further: After the turbidity measurement and / or dilution treatment of the cell suspension in a centrifuge tube is completed, the cleaning component is enabled. The horizontal and vertical movement module drives the moving tube seat to move forward and backward and descend, so that the sampling tube extends into the cleaning liquid tank from the cleaning inlet, and the drain pipe extends into the waste liquid storage tank from the sewage discharge inlet. The first shut-off valve and the second shut-off valve are controlled to open simultaneously, and then the drive source is started, so that the cleaning liquid in the cleaning liquid tank enters the sample delivery pipe through the sampling tube, then enters the mixing and shunting pipe and the colorimetric cell, and finally flows to the waste liquid storage tank through the discharge pipe and the drain pipe for automatic cleaning.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. In this application, the automatic measurement of the cell suspension concentration is realized through the turbidity detection mechanism, and the closed-loop dilution adjustment is completed in combination with the dilution adjustment mechanism, effectively improving the accuracy of concentration control and operation efficiency;

[0032] 2. The coordinated cooperation between the carrier conveyor line and the pipetting mechanism in this application realizes the automated process from the centrifuge tube to the glass slide, reduces manual intervention, and reduces the operation complexity and human error;

[0033] 3. This application integrates the functions of concentration detection, dilution adjustment, and precise dropwise dispensing on a glass slide, significantly improving the standardization level and overall efficiency of the experimental process, and meeting the requirements of biomedical experiments for high precision and high efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 is a schematic structural diagram of the device for automatically measuring cell concentration and dropping on a glass slide of the present invention;

[0035] Figure 2 is a schematic structural diagram of the dilution liquid dropping control module of the present invention;

[0036] Figure 3 is Figure 1 a partial enlarged structural diagram at position A;

[0037] Figure 4 is a schematic structural diagram of the pipetting mechanism of the present invention;

[0038] Figure 5 is an exploded schematic structural diagram of the glass slide platform of the present invention;

[0039] Figure 6 is a schematic structural diagram of the carrier of the present invention.

[0040] Description of reference numerals: 1 - frame, 2 - dilution adjustment station, 3 - turbidity detection station, 4 - pipetting station, 5 - dropping plate station, 6 - carrier conveyor line, 7 - dilution adjustment mechanism, 8 - dilution liquid dropping control module, 9 - cell suspension conveying module, 10 - horizontal adjustment module, 11 - horizontal and vertical movement module, 12 - turbidity detection mechanism, 13 - pipetting mechanism, 14 - three-dimensional movement module, 15 - pipette, 16 - lifting module, 17 - lifting plate, 18 - glass slide table, 19 - identification scanning device, 20 - first visual identification, 21 - second visual identification, 22 - liquid storage tank, 23 - dropping tube, 24 - flow control part, 25 - flow sensor, 26 - horizontal movement module, 27 - lifting seat, 28 - colorimetric cuvette, 29 - turbidimeter, 30 - moving tube seat, 31 - sampling tube, 32 - sample conveying tube, 33 - drain pipe, 34 - discharge pipe, 35 - first interface, 36 - second interface, 37 - drive source, 38 - mixing and shunting pipe, 39 - first shut-off valve, 40 - second shut-off valve, 41 - one-way valve, 42 - table body, 43 - cover plate, 44 - placement groove, 45 - dropping port, 46 - material taking groove, 47 - guiding chute, 48 - bearing frame, 49 - frame conveyor line, 50 - upper support plate, 51 - lower support plate, 52 - support plate, 53 - positioning groove, 54 - alignment groove, 55 - cleaning liquid tank, 56 - waste liquid storage tank, 57 - cleaning inlet, 58 - sewage discharge inlet, 59 - centrifuge tube, 60 - glass slide. Detailed implementation manners

[0041] Figures 1 to 6 The following is a schematic structural diagram of an embodiment of a dropping plate device for automatically measuring cell concentration provided by the present invention, including a frame 1. A dilution adjustment station 2, a turbidity detection station 3, a pipetting station, and a dropping plate station 5 are sequentially arranged on the frame 1 according to the operation sequence. A carrier conveyor line 6 for orienting and conveying the centrifuge tube 59 along each station is arranged on the frame 1.

[0042] A dilution adjustment mechanism 7 is arranged at the dilution adjustment station 2. The dilution adjustment mechanism 7 includes a dilution liquid dropping control module 8 and a cell suspension conveying module 9. The dilution liquid dropping control module 8 is connected to a horizontal adjustment module 10, and the cell suspension conveying module 9 is connected to a horizontal and vertical movement module 11.

[0043] A turbidity detection mechanism 12 is arranged at the turbidity detection station 3, and the cell suspension conveying module 9 is communicated with the turbidity detection mechanism 12.

[0044] A pipetting mechanism 13 is arranged at the pipetting station. The pipetting mechanism 13 includes a three-dimensional movement module 14 and a pipette 15. The three-dimensional movement module 14 is arranged on the frame 1, and the pipette 15 is arranged on the three-dimensional movement module 14.

[0045] The three-dimensional movement module 14 includes a lifting module 16. The lifting module 16 includes a lifting plate 17. The pipettor 15 is fixedly connected to the lifting plate 17 in the lifting module 16. The pipettor 15 can move along the X, Y, and Z directions through the three-dimensional movement module, thereby achieving precise positioning. In this embodiment, the pipettor 15 is specifically an electric pipettor 15, which can provide higher liquid handling precision and accuracy. In actual operation, disposable tips are used on the electric pipettor 15 to avoid cross-contamination.

[0046] A glass slide stage 18 is provided at the dropping slide position 5. The glass slide stage 18 is used to carry the glass slide 60.

[0047] An identification scanning device 19 is provided beside the pipettor 15. A first visual identification 20 is pasted on the glass slide 60, and a second visual identification 21 is pasted on the centrifuge tube 59. The first visual identification 20 and the second visual identification 21 correspond to each other one by one.

[0048] The first visual identification 20 can be a QR code or a barcode, which contains information such as the centrifuge tube 59 number and sample information. The second visual identification 21 is used to label the glass slide 60 to ensure the accuracy of the pipetting operation. The identification scanning device 19 can be a laser scanner or a CCD camera. By introducing visual identification, automatic identification and tracking of sample information are realized, and the intelligent level of experimental operation is improved. At the same time, the coordinated work of the identification scanning device 19 with the three-dimensional movement module 14 and the pipettor 15 ensures the accuracy of the pipetting operation and avoids the hidden danger of specimen result errors caused by human errors.

[0049] The diluent dropping control module 8 includes a liquid storage tank 22, a dropping tube 23, a flow control member 24, and a flow sensor 25. The liquid storage tank 22 is arranged on the horizontal adjustment module 10. The liquid storage tank 22 is used to store the diluent. The dropping tube 23 is communicated with the bottom of the liquid storage tank 22. The flow control member 24 and the flow sensor 25 are respectively arranged on the dropping tube 23, and the flow sensor 25 is located behind the flow control member 24.

[0050] In this embodiment, the diluent is usually physiological saline or other buffer solutions. The material of the liquid storage tank 22 can be polypropylene or polyethylene, which has high chemical stability. The flow control member 24 is specifically a proportional valve. The flow control member 24 and the flow sensor 25 are electrically connected. The operating state of the flow control member 24 can be controlled by the drainage volume detected by the flow sensor 25 to precisely control the liquid flow. A vent valve is also communicated with the top of the liquid storage tank 22 to facilitate the smooth discharge of the diluent in the liquid storage tank 22.

[0051] The turbidity detection mechanism 12 includes a colorimetric cell 28 and a turbidimeter 29. The turbidimeter 29 is fixed on the frame 1. The colorimetric cell 28 is arranged in the detection area of the turbidimeter 29, and the colorimetric cell 28 is made of a transparent material. In this embodiment, the colorimetric cell 28 is fixed in the detection area of the turbidimeter 29 through a bracket. The colorimetric cell 28 can be made of glass or quartz to ensure that light can penetrate the liquid sample smoothly.

[0052] The cell suspension delivery module 9 includes a movable pipe seat 30, a sampling pipe 31, a sample delivery pipe 32, a drain pipe 33, and a discharge pipe 34. The movable pipe seat 30 is connected to the horizontal and vertical movement module 11. The sample delivery pipe 32 and the discharge pipe 34 are both flexible hoses. The sampling pipe 31 and the drain pipe 33 are arranged in parallel at the bottom of the movable pipe seat 30, and the length of the sampling pipe 31 is greater than the length of the drain pipe 33. One end of the sample delivery pipe 32 penetrates into the movable pipe seat 30 and is connected to the sampling pipe 31. The other end of the sample delivery pipe 32 is connected to the first interface 35 of the colorimetric cell 28. One end of the discharge pipe 34 penetrates into the movable pipe seat 30 and is connected to the drain pipe 33. The other end of the discharge pipe 34 is connected to the second interface 36 of the colorimetric cell 28. A driving source 37 is arranged on the sample delivery pipe 32.

[0053] The horizontal and vertical movement module 11 includes a horizontal movement module 26 and a lifting seat 27. The slider of the horizontal movement module 26 is connected to the lifting seat 27, and the lifting seat 27 is fixed to the movable pipe seat 30. In this embodiment, both the horizontal movement module 26 and the horizontal adjustment module 10 are selected as ball screw slide modules, and the lifting seat 27 is selected as an electric push rod.

[0054] In this embodiment, the sampling pipe 31 is designed such that its length is greater than the length of the drain pipe 33. When the sampling pipe 31 and the drain pipe 33 simultaneously extend into the centrifuge tube 59, the drain pipe 33 does not extend into the liquid level in the centrifuge tube 59.

[0055] Sealing rings are sleeved at both the first interface 35 and the second interface 36 of the colorimetric cell 28 to prevent liquid leakage. The size of the colorimetric cell 28 is selected as 1 mm.

[0056] This embodiment further includes a mixing and shunting pipe 38. One end of the mixing and shunting pipe 38 is connected to the part of the sample delivery pipe 32 between the turbidimeter 29 and the driving source 37. The other end of the mixing and shunting pipe 38 is connected to the part of the discharge pipe 34 close to the movable pipe seat 30. A first shut-off valve 39 is arranged on the mixing and shunting pipe 38, and the first shut-off valve 39 is used to control the on-off between the mixing and shunting pipe 38 and the sample delivery pipe 32. A second shut-off valve 40 is arranged on the sample delivery pipe 32, and the second shut-off valve 40 is used to control the on-off between the sample delivery pipe 32 and the colorimetric cell 28. A one-way valve 41 is arranged on the discharge pipe 34, and the one-way valve 41 is close to the connection between the discharge pipe 34 and the mixing and shunting pipe 38. The one-way valve 41 is used to prevent the liquid in the mixing and shunting pipe 38 from flowing towards the colorimetric cell 28.

[0057] A one-way valve 41 is provided on the discharge pipe 34, and the one-way valve 41 is located near the connection between the discharge pipe 34 and the mixing and shunting pipe 38 to prevent the liquid in the mixing and shunting pipe 38 from flowing back into the cuvette 28. The one-way valve 41 can be selected as a spring type structure, which has high sealing performance and reliability.

[0058] When mixing the diluent and the cell suspension, by opening the first shut-off valve 39 and simultaneously closing the second shut-off valve 40, under the action of the driving source 37, the liquid in the centrifuge tube 59 is extracted through the sampling pipe 31 to the sample delivery pipe 32, and then flows back into the centrifuge tube 59 through the mixing and shunting pipe 38, the discharge pipe 34 and the drain pipe 33. Such a cycle ensures the uniform mixing of the diluent and the cell suspension, ensures the accuracy of the detection of the concentration of the diluted cell suspension. At the same time, the design of the one-way valve 41 effectively avoids the phenomenon of liquid backflow, ensuring the stability and safety of the system.

[0059] The glass slide stage 18 includes a stage body 42 and a cover plate 43 provided on the stage body 42. A plurality of placement grooves 44 are formed on the stage body 42, and a plurality of liquid dropping ports 45 are formed on the cover plate 43. The liquid dropping ports 45 and the placement grooves 44 are arranged in one-to-one correspondence. A material taking groove 46 is provided at the notch of the placement groove 44, and a guiding sliding groove 47 is provided above the material taking groove 46.

[0060] When placing or removing the glass slide 60, the glass slide 60 can be moved along the guiding sliding groove 47 of the placement groove 44 to improve the stability of placing and removing the glass slide 60.

[0061] The stage body 42 can be made of aluminum alloy or stainless steel, which has high strength and stability. Setting the cover plate 43 on the stage body 42 can effectively prevent dust from contaminating the surface of the glass slide 60. The color plate is made of a transparent material.

[0062] The carrier conveyor line 6 includes a carrier frame 48 and a frame conveyor line 49. The carrier frame 48 is movably arranged on the frame conveyor line 49. The carrier frame 48 includes an upper support plate 50 and a lower support plate 51. A support plate 52 is connected between the upper support plate 50 and the lower support plate 51 to form an I-shaped structure. A plurality of positioning grooves 53 are formed on the upper support plate 50, and a plurality of alignment grooves 54 are formed on the lower support plate 51. The positioning grooves 53 and the alignment grooves 54 are arranged in one-to-one correspondence.

[0063] The inner wall of the alignment groove 54 is designed in a conical shape and is closely attached to the outer wall of the centrifuge tube 59. When carrying the centrifuge tube 59, the centrifuge tube 59 is positioned jointly by the positioning groove 53 and the alignment groove 54 to prevent the centrifuge tube 59 from shaking.

[0064] A buffer pad is provided on the inner wall of the alignment groove 54. The buffer pad can be selected from rubber or silica gel materials, which has good shock absorption effect to avoid damage to the centrifuge tube 59 due to vibration.

[0065] In this embodiment, the positioning grooves 53, alignment grooves 54, and placement grooves 44 are equal in number, all six are provided, and are distributed in two rows.

[0066] The frame transfer line 49 drives the carrier frame 48 to drive the centrifuge tube 59 to move in the direction of the glass slide stage 18. The frame transmission line can adopt a belt drive or a chain drive method, with high running accuracy and reliability. In this embodiment, the frame transfer line 49 is specifically selected as a belt transmission and driven by a stepper motor.

[0067] In order to continuously detect the turbidity of the cell suspension in multiple centrifuge tubes 59, a cleaning assembly is further included. The cleaning assembly includes a cleaning liquid tank 55 and a waste liquid storage tank 56. A cleaning inlet 57 is opened at the top of the cleaning liquid tank 55, and a sewage discharge inlet 58 is opened at the top of the waste liquid storage tank 56.

[0068] The cleaning liquid in the cleaning liquid tank 55 can be selected as deionized water or a special cleaning agent, which has strong decontamination ability.

[0069] After the turbidity detection mechanism 12 and the dilution adjustment mechanism 7 finish processing the cell suspension in a centrifuge tube 59, it is necessary to clean the sampling tube 31, the sample delivery tube 32, the mixing and shunting tube 38, and the colorimetric cell 28. At this time, the tube seat 30 is moved by the horizontal movement module 26 and the lifting seat 27 to drive the sampling tube 31 and the drain pipe 33 to move, so that the sampling tube 31 enters the cleaning liquid tank 55 along the cleaning inlet 57, and at the same time, the discharge pipe 34 also enters the waste liquid storage tank 56 along the sewage discharge inlet 58; at the same time, the first shut-off valve 39 and the second shut-off valve 40 are controlled to connect the sample delivery tube 32 to both the mixing and shunting tube 38 and the colorimetric cell 28, and then the drive source 37 is started to drive the cleaning liquid in the cleaning liquid tank 55 to flow along the sampling tube 31 to the mixing and shunting tube 38 and the colorimetric cell 28, and finally flow along the drain pipe 33 into the waste liquid storage tank 56. The automatic cleaning function is realized, the manual maintenance cost is reduced, and the service life of the equipment is extended. At the same time, the effective management of the cleaning liquid and the waste liquid ensures the cleanliness and safety of the experimental environment.

[0070] A method for using an automatic cell concentration measuring and dropping device includes the following steps:

[0071] S1. Insert the centrifuge tube containing the cell suspension into the positioning groove of the carrier frame, place the glass slide in the placement groove of the glass slide stage. At the same time, a first visual identification is pasted on the glass slide, and a second visual identification is pasted on the centrifuge tube.

[0072] S2. Drive the carrier frame to move along the frame transfer line and move the carrier frame to the dilution adjustment station.

[0073] S3. The horizontal and vertical movement module drives the moving tube seat to move back and forth and descend, so that the sampling tube extends below the liquid level in the centrifuge tube, and the drive source is turned on to extract the cell suspension in the centrifuge tube, and the cell suspension is transported to the colorimetric cell by the sample delivery tube.

[0074] S4. The turbidimeter measures the light transmittance and calculates the turbidity value. If the detected turbidity of the cell suspension is less than or equal to the preset value, the turbidity of the sample solution meets the standard, and there is no need to dilute the cell suspension. It can directly enter the pipetting station. If the detected turbidity of the cell suspension is greater than the preset value, step S5 is executed.

[0075] S5. Open the flow control component, automatically calculate the required amount of diluent according to the turbidity difference, and monitor the flow rate in real time through the flow sensor to form a closed-loop control. The horizontal adjustment module drives the liquid storage tank to move back and forth, align the dropper with the corresponding centrifuge tube, and make the diluent in the liquid storage tank drip into the corresponding centrifuge tube through the dropper. Then, open the first shut-off valve, and at the same time, close the second shut-off valve. Under the action of the driving source, the liquid in the centrifuge tube is extracted to the sample delivery tube through the sampling tube, and then flows back to the centrifuge tube through the mixing and shunting tube, the discharge tube and the drain tube. Repeat this three times to mix the diluent and the cell suspension.

[0076] S6. Extract the mixed sample solution of the diluent and the cell suspension for turbidity retest until the turbidity meets the standard and enter the pipetting station.

[0077] S7. After the qualified cell suspension is transferred to the pipetting station, the three-dimensional movement module drives the pipette to aspirate the sample solution in the centrifuge tube according to the set amount. At the same time, the identification scanning device scans the first visual identification on the corresponding centrifuge tube and the second visual identification on the glass slide on the stage. The three-dimensional movement module drives the pipette to move to the target dropping position, and the pipette drops the sample solution along the corresponding dropping port onto the corresponding glass slide.

[0078] Further: When the turbidity measurement and / or dilution treatment of the cell suspension in a centrifuge tube is completed, enable the cleaning component. The horizontal and vertical movement module drives the moving tube seat to move back and forth and descend, so that the sampling tube extends into the cleaning liquid tank from the cleaning inlet, and the drain tube extends into the waste liquid storage tank from the sewage discharge inlet. Control the first shut-off valve and the second shut-off valve to open at the same time, and then start the driving source, so that the cleaning liquid in the cleaning liquid tank enters the sample delivery tube through the sampling tube, then enters the mixing and shunting tube and the colorimetric cell, and finally flows to the waste liquid storage tank through the discharge tube and the drain tube for automatic cleaning.

[0079] The above detailed description is a specific description of the feasible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification without departing from the present invention should be included in the patent scope of this case.

Claims

1. An automatic cell concentration measuring dropping device, characterized in that: It includes a frame, on which a dilution adjustment station, a turbidity detection station, a pipetting station, and a drop-slicing station are sequentially arranged according to the operation sequence, and a carrier conveyor line for directionally conveying centrifuge tubes along each station is arranged on the frame; A dilution adjustment mechanism is arranged at the dilution adjustment station. The dilution adjustment mechanism includes a dilution liquid dropping control module and a cell suspension conveying module. The dilution liquid dropping control module is connected to a horizontal adjustment module, and the cell suspension conveying module is connected to a horizontal and vertical movement module; A turbidity detection mechanism is arranged at the turbidity detection station, and the cell suspension conveying module is communicated with the turbidity detection mechanism; A pipetting mechanism is arranged at the pipetting station. The pipetting mechanism includes a three-dimensional movement module and a pipettor. The three-dimensional movement module is arranged on the frame, and the pipettor is arranged on the three-dimensional movement module; A glass slide stage is arranged at the drop-slicing station, and the glass slide stage is used for carrying glass slides; An identification scanning device is arranged beside the pipettor. A first visual identification is pasted on the glass slide, and a second visual identification is pasted on the centrifuge tube. The first visual identification and the second visual identification correspond to each other one by one.

2. The drop plate device for automatically measuring cell concentration according to claim 1, wherein: The dilution liquid dropping control module includes a liquid storage tank, a dropping tube, a flow control component, and a flow sensor. The liquid storage tank is arranged on the horizontal adjustment module and is used for storing dilution liquid. The dropping tube is communicated with the bottom of the liquid storage tank. The flow control component and the flow sensor are respectively arranged on the dropping tube, and the flow sensor is located behind the flow control component.

3. The drop-sheet device for automatically measuring cell concentration according to claim 2, wherein: The turbidity detection mechanism includes a colorimetric cell and a turbidimeter. The turbidimeter is fixed on the frame, the colorimetric cell is arranged in the detection area of the turbidimeter, and the colorimetric cell is made of a transparent material.

4. The automatic cell concentration measuring dropper device according to claim 3, wherein: The cell suspension conveying module includes a moving tube seat, a sampling tube, a sample conveying tube, a drainage tube, and a discharge tube. The moving tube seat is connected to the horizontal and vertical movement module. The sample conveying tube and the discharge tube are both flexible tubes. The sampling tube and the drainage tube are arranged in parallel at the bottom of the moving tube seat, and the length of the sampling tube is greater than the length of the drainage tube. One end of the sample conveying tube penetrates into the moving tube seat and is communicated with the sampling tube. The other end of the sample conveying tube is communicated with the first interface of the colorimetric cell. One end of the discharge tube penetrates into the moving tube seat and is communicated with the drainage tube. The other end of the discharge tube is communicated with the second interface of the colorimetric cell. A driving source is arranged on the sample conveying tube.

5. The dropper device for automatically measuring cell concentration according to claim 4, characterized in that: It further includes a mixing and diverting pipe. One end of the mixing and diverting pipe is communicated with the part of the sample conveying pipe between the turbidimeter and the driving source, and the other end of the mixing and diverting pipe is communicated with the part of the discharge pipe close to the movable pipe seat. A first shut-off valve is arranged on the mixing and diverting pipe, and the first shut-off valve is used to control the on-off between the mixing and diverting pipe and the sample conveying pipe. A second shut-off valve is arranged on the sample conveying pipe, and the second shut-off valve is used to control the on-off between the sample conveying pipe and the colorimetric cell. A one-way valve is arranged on the discharge pipe, and the one-way valve is close to the connection part of the discharge pipe and the mixing and diverting pipe. The one-way valve is used to prevent the liquid in the mixing and diverting pipe from flowing to the colorimetric cell.

6. The automatic cell concentration measuring dropping device according to claim 5, characterized in that: The glass slide stage includes a stage body and a cover plate arranged on the stage body. A plurality of placement grooves are formed on the stage body, and a plurality of liquid dropping ports are formed on the cover plate. The liquid dropping ports and the placement grooves are arranged in one-to-one correspondence. A material taking groove is arranged at the notch of the placement groove, and a guiding sliding groove is arranged above the material taking groove.

7. The automatic cell concentration measuring dropping device according to claim 5, characterized in that: The carrying and conveying line includes a carrying frame and a frame conveyor line. The carrying frame is movably arranged on the frame conveyor line. The carrying frame includes an upper supporting plate and a lower supporting plate. A supporting plate is connected between the upper supporting plate and the lower supporting plate to form an I-shaped structure. A plurality of positioning grooves are formed on the upper supporting plate, and a plurality of alignment grooves are formed on the lower supporting plate. The positioning grooves and the alignment grooves are arranged in one-to-one correspondence.

8. The drop-sheet device for automatically measuring cell concentration according to claim 7, wherein: It further includes a cleaning assembly. The cleaning assembly includes a cleaning liquid tank and a waste liquid storage tank. A cleaning inlet is formed at the top of the cleaning liquid tank, and a sewage discharge inlet is formed at the top of the waste liquid storage tank.

9. A method for using a dropping device for automatically measuring cell concentration according to any one of claims 6-8, characterized in that: It includes the following steps S1. Insert the centrifuge tube filled with cell suspension into the positioning groove of the carrying frame, and place the glass slide in the placement groove of the glass slide stage. At the same time, a first visual identifier is pasted on the glass slide, and a second visual identifier is pasted on the centrifuge tube; S2. Drive the carrying frame to move along the frame conveyor line and move the carrying frame to the dilution adjustment station; S3. The horizontal and vertical movement module drives the movable pipe seat to move forward and backward and descend, so that the sampling pipe extends below the liquid level in the centrifuge tube. The driving source is turned on to extract the cell suspension in the centrifuge tube, and the cell suspension is conveyed to the colorimetric cell by the sample conveying pipe; S4. The turbidimeter measures the transmittance and calculates the turbidity value. If it is detected that the turbidity of the cell suspension is less than or equal to the preset value, the turbidity of the sample liquid meets the standard, and there is no need to dilute the cell suspension, and it can directly enter the pipetting station. If it is detected that the turbidity of the cell suspension is greater than the preset value, step S5 is executed; S5. Turn on the flow control part, automatically calculate the required amount of diluent according to the turbidity difference, and monitor the flow rate in real time through the flow sensor to form a closed-loop control. The horizontal adjustment module drives the liquid storage tank to move forward and backward, so that the dropping pipe is aligned with the corresponding centrifuge tube, and the diluent in the liquid storage tank is dropped into the corresponding centrifuge tube through the dropping pipe. Then, the first shut-off valve is opened, and at the same time, the second shut-off valve is closed. Under the action of the driving source, the liquid in the centrifuge tube is extracted to the sample conveying pipe through the sampling pipe, and then flows back to the centrifuge tube through the mixing and diverting pipe, the discharge pipe and the drain pipe. This cycle is repeated three times to mix the diluent and the cell suspension; S6. Take a mixed sample of the diluent and the cell suspension for turbidity retest until the turbidity meets the standard, and then enter the pipetting station; S7. After the qualified cell suspension is transferred to the pipetting station, the three-dimensional movement module drives the pipette to aspirate the sample solution in the centrifuge tube according to the set amount. At the same time, the identification scanning device scans the first visual identification of the corresponding centrifuge tube and the second visual identification on the glass slide on the glass slide stage. The three-dimensional movement module drives the pipette to move to the target dropping position, and the pipette drops the sample solution along the corresponding dropping port onto the corresponding glass slide.

10. The usage method of a dropper device for automatically measuring cell concentration according to claim 9, characterized in that: When the turbidity measurement and / or dilution treatment of the cell suspension in a centrifuge tube is completed, enable the cleaning component. The horizontal and vertical movement module drives the movable tube seat to move forward and backward and descend, so that the sampling tube extends into the cleaning liquid tank from the cleaning inlet, and the drain pipe extends into the waste liquid storage tank from the sewage discharge inlet. Control the first shut-off valve and the second shut-off valve to open simultaneously, and then start the drive source, so that the cleaning liquid in the cleaning liquid tank enters the sample delivery pipe through the sampling tube, then enters the mixing and shunting pipe and the colorimetric cell, and finally flows to the waste liquid storage tank through the discharge pipe and the drain pipe for automatic cleaning.

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