A traditional Chinese medicine composition for preventing and treating poultry adenovirus and its pharmaceutical preparation

Abstract

The present invention relates to a traditional Chinese medicine composition and pharmaceutical preparation for preventing and treating poultry adenovirus, belonging to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 23-37 parts of Pittosporum tobira bark, 23-37 parts of Artemisia capillaris, 15-25 parts of Radix Isatidis, 5-15 parts of Flos Lonicerae, 5-15 parts of Cuscuta australis, 5-11 parts of Ecliptae chinensis, 5-11 parts of Fructus Aurantii Immaturus, and 3-9 parts of Caulis Polygoni Multiflori. Pharmacodynamic experiments have confirmed that the traditional Chinese medicine composition can significantly improve symptom scores. The prepared oral liquid has a stable chlorogenic acid content and exhibits good chemical stability under accelerated conditions, making it suitable for further promotion and application.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and relates to a traditional Chinese medicine composition for preventing and treating poultry adenovirus and a pharmaceutical preparation thereof. Background Art

[0002] In recent years, global outbreaks of group I avian adenovirus (FAdV) have occurred, causing significant economic losses to the poultry industry. Group I FAdV can cause a variety of diseases in poultry, including inclusion body hepatitis (IBH), hydropericardium syndrome (HPS), and gizzard erosion (GE). The prevalence of group I FAdV in my country can be divided into three phases: the first, from 1976 to 2007, was characterized by sporadic outbreaks of IBH in affected poultry, with minimal losses. The second, from 2007 to 2014, was characterized by endemic outbreaks, with IBH still predominant, and infection rates reaching 30% and mortality rates reaching 15%. The third phase, which began in 2015, saw widespread outbreaks in my country, primarily characterized by IBH and HPS, leading to outbreaks. Summary of the Invention

[0003] In view of the shortcomings of the prior art, the present invention provides a traditional Chinese medicine composition and pharmaceutical preparation primarily containing Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Flos Lonicerae, Semen Cuscutae, Ecliptae, Fructus Aurantii, and Caulis Polygoni Multiflori. These compositions address the challenge of poultry adenovirus infection and offer significant economic benefits. The traditional Chinese medicine composition of the present invention can be formulated in the form of a powder, oral liquid, or granules, all of which achieve the same or similar technical effects.

[0004] Specifically, the present invention adopts the following technical solutions to achieve the above objectives:

[0005] A traditional Chinese medicine composition for preventing and treating poultry adenovirus is prepared from the following raw materials in parts by weight: 23-37 parts of Pittosporum tobira bark, 23-37 parts of Artemisia capillaris, 15-25 parts of Radix Isatidis, 5-15 parts of Flos Lonicerae, 5-15 parts of Cuscuta australis, 5-11 parts of Eclipta prostrata, 5-11 parts of Fructus Aurantii Immaturus, and 3-9 parts of Caulis Polygoni Multiflori.

[0006] Further preferably, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 37 parts of Pittosporum tobira bark, 37 parts of Artemisia capillaris, 25 parts of Isatis indigotica, 15 parts of Honeysuckle, 15 parts of Cuscuta australis, 11 parts of Eclipta prostrata, 11 parts of Citrus aurantium, and 9 parts of Polygonum multiflorum.

[0007] Further preferably, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 30 parts of Pittosporum tobira bark, 30 parts of Artemisia capillaris, 20 parts of Isatis root, 10 parts of Honeysuckle, 10 parts of Cuscuta australis, 8 parts of Eclipta prostrata, 8 parts of Citrus aurantium, and 6 parts of Polygonum multiflorum.

[0008] Further preferably, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 23 parts of Pittosporum tobira bark, 23 parts of Artemisia capillaris, 15 parts of Isatis indigotica, 5 parts of Honeysuckle, 5 parts of Cuscuta australis, 5 parts of Ecliptae, 5 parts of Citrus aurantium, and 3 parts of Polygonum multiflorum.

[0009] A pharmaceutical preparation for preventing and treating poultry adenovirus, comprising the traditional Chinese medicine composition of the present invention and pharmaceutically acceptable excipients.

[0010] Furthermore, the pharmaceutical preparation is selected from any one of powder, oral liquid or granule.

[0011] Furthermore, the pharmaceutical preparation is selected from oral liquid.

[0012] Furthermore, the preparation method of the oral liquid comprises the following steps:

[0013] (1) Add 8-15 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 4-8 hours; simmer under steam reflux for 1.5-2.5 hours, filter to obtain a filtrate and a filter residue; add 4-8 times the weight of water to the filter residue, simmer under steam reflux for 0.5-1.5 hours, filter, combine the two filtrates, and use the filter residue for later use;

[0014] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue of step (1), add water and boil twice for 0.5-1.5 hours, combine the two decoctions, filter, and obtain a filtrate;

[0015] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.02-1.05 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0016] (4) Add 0.1-0.3% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 4.5-5.5 to obtain the product.

[0017] Furthermore, the preparation method of the oral liquid comprises the following steps:

[0018] (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0019] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0020] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0021] (4) Add 0.2% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The Chinese medicine composition of the present invention has all the monarch, minister, assistant and guiding herbs, which is effective in treating various diseases caused by heat and toxicity stagnation. The Artemisia capillaris can clear away heat and toxicity, promote dampness and reduce jaundice, and the two are the monarch herbs. The honeysuckle is a heat-clearing and detoxifying herb, and together with the Radix Isatidis, it plays a heat-clearing and detoxifying role. These two herbs complement each other with the monarch herbs, and can enhance the overall heat-clearing and detoxifying effect. The dodder seed, as the minister, has the effect of enhancing the body's resistance and avoiding the deficiency caused by excessive heat-clearing and detoxification. The addition of the dodder seed has the effect of harmonizing the medicinal properties and nourishing the liver and kidneys, avoiding the excessive coldness or excessive heat-clearing effect of the medicine, making the overall effect of the prescription more balanced. The addition of Eclipta prostrata and Fructus aurantii can promote the smooth flow of qi, and the effect of promoting blood circulation and regulating qi in combination with the Pittosporum tobirica can harmonize qi and blood, and reduce the discomfort caused by excessive heat-clearing. Finally, the addition of Polygonum multiflorum can coordinate the prescription, so that the functions of other Chinese herbs can be exerted and guide other herbs to play their role in heat-clearing and detoxifying.

[0024] 2) Pharmacodynamic experiments confirmed that the Chinese medicinal composition of the present invention can significantly increase the body weight and symptom score of white-feathered chickens as shown in the body weight test in the first week. There are significant differences between Examples 1-3 and Comparative Examples 1-4 (P < 0.01 or P < 0.0001).

[0025] 3) The preferred extraction and preparation process of the present invention ensures efficient extraction of chlorogenic acid by carefully controlling extraction conditions such as temperature, time, and concentration. The chlorogenic acid content in the prepared oral solution is above 45 mg / g. Accelerated stability testing showed that the chlorogenic acid content remained stable, with no significant changes. The inventors believe that this may be due to the formation of a relatively stable system by butylated hydroxyanisole, thereby protecting the chlorogenic acid from degradation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 : Experiment on the effect of Chinese medicine compositions of different embodiment components on the body weight of white-feather chickens at the initial stage, the first week and the second week.

[0027] Figure 2 : Experiment on the effect of Chinese medicine compositions of different embodiment components on symptom scores of white-feathered chickens.

[0028] Figure 3 : The chlorogenic acid content of Example 1, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Comparative Example 8 at the 0th month, 1st month, 2nd month, 3rd month and 6th month after accelerated testing. DETAILED DESCRIPTION

[0029] The present invention is further illustrated below with reference to specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention made by those skilled in the art all fall within the scope of protection of the claims of this application.

[0030] Example 1: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0031] Pittosporum tobira bark 30g, Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0032] (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0033] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0034] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0035] (4) Add 0.2% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.0.

[0036] Example 2: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0037] Pittosporum tobira bark 23g, Artemisia capillaris 23g, Isatis root 15g, Honeysuckle 5g, Cuscuta australis 5g, Eclipta prostrata 5g, Citrus aurantium 5g, Polygonum multiflorum 3g.

[0038] (1) Add 8 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 4 hours; steam reflux and decoct for 1.5 hours, filter to obtain a filtrate and a filter residue; add 4 times the weight of water to the filter residue, steam reflux and decoct for 0.5 hours, filter, combine the two filtrates, and use the filter residue for later use;

[0039] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 0.5 hours, combine the two decoctions, filter, and obtain a filtrate;

[0040] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.02 at 80°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0041] (4) Add 0.1% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 4.5.

[0042] Example 3: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0043] Pittosporum tobira bark 37g, Artemisia capillaris 37g, Isatis root 25g, Honeysuckle 15g, Cuscuta australis 15g, Eclipta prostrata 11g, Citrus aurantium 11g, Polygonum multiflorum 9g.

[0044] (1) Add 15 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 8 hours; steam reflux and decoct for 2.5 hours, filter to obtain a filtrate and a filter residue; add 8 times the weight of water to the filter residue, steam reflux and decoct for 1.5 hours, filter, combine the two filtrates, and use the filter residue for later use;

[0045] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1.5 hours, combine the two decoctions, filter, and obtain a filtrate;

[0046] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.05 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral solution;

[0047] (4) Add 0.1-0.3% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.5.

[0048] Comparative Example 1: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0049] Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0050] (1) Add 13 times the weight of water to Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori and soak for 6 hours; steam reflux and decoct for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and decoct for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0051] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0052] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0053] (4) Add 0.2% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.0.

[0054] Comparative Example 2: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0055] Pittosporum tobira bark 15g, Artemisia capillaris 45g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0056] (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0057] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0058] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0059] (4) Add 0.2% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.0.

[0060] Comparative Example 3: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0061] 90g of Salvia miltiorrhiza, 90g of Agrimoniae, 30g of Herba Euphorbiae, 30g of Poria, 30g of Red Ochre, and 30g of Licorice.

[0062] (1) The raw material components of the above-mentioned pharmaceutical preparation for preventing and treating poultry group I adenovirus (90g each of Salvia miltiorrhiza and Agrimoniae scabra, 30g each of Euphorbia pulex, Poria cocos, Erythrorhizon, and Licorice) were crushed and mixed, added to an extraction tank, poured into clean water, soaked and rinsed to remove impurities and dust; 8 times the weight of clean water was added to the rinsed raw material components, and soaked for 8 hours; then, steam refluxed and boiled for 3 hours, filtered, and obtained a filtrate and a filter residue; 4 times the weight of clean water was added to the filter residue, steam refluxed and boiled for 2 hours, filtered, and the two filtrates were combined;

[0063] (2) concentrating the filtrate obtained in step (1) to a specific gravity of 1.15, and adjusting the pH to 5.0 with 1% by mass hydrochloric acid to obtain a pharmaceutical preparation for preventing and treating poultry group I adenovirus;

[0064] (3) Sodium benzoate is added to the pharmaceutical preparation for preventing and treating poultry group I adenovirus prepared in step (2) for preservation to obtain a finished pharmaceutical preparation for preventing and treating poultry group I adenovirus, wherein the mass fraction of sodium benzoate in the finished pharmaceutical preparation for preventing and treating poultry group I adenovirus is 0.3%.

[0065] Comparative Example 4: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0066] Isatis root 15g, white peony root 10g, Artemisia capillaris 20g, Gentiana 10g, Codonopsis pilosula 7.5g, Poria 7.5g, Scutellaria baicalensis 10g, Sophora flavescens 10g, Licorice root 10g, Plantago asiatica 15 fen, and Centella asiatica 20g.

[0067] The above raw materials are crushed and passed through a 300-mesh sieve to obtain a pharmaceutical preparation, which is decocted twice with water for 1 hour. The two decoctions are combined and filtered to obtain a filtrate, which is concentrated at 80-90° C. to a relative density of 1.03 and cooled to room temperature to obtain a Chinese medicine oral liquid; 0.2% of the total weight of butylated hydroxyanisole is added, and finally citric acid is added to adjust the pH value to 5.0 to obtain the product.

[0068] Comparative Example 5: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0069] Pittosporum tobira bark 30g, Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0070] Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Flos Lonicerae, Semen Cuscutae, Ecliptae, Fructus Aurantii Immaturus, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and decoct for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and decoct for 1 hour, filter, combine the two filtrates, concentrate at 80-90° C. to a relative density of 1.03, cool to room temperature, and obtain a Chinese medicine oral liquid; add 0.2% of the total weight of butylated hydroxyanisole, and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0071] Comparative Example 6: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0072] Pittosporum tobira bark 30g, Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0073] Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Cuscuta australis, Caulis Polygoni Multiflori, Flos Lonicerae, Eclipta prostrata, and Fructus Aurantii Immaturus are ground and mixed, added into ethanol and refluxed for 3 hours, concentrated to a relative density of 1.03 at 40-45° C., cooled to room temperature, and a Chinese medicine oral liquid is obtained; 0.2% by weight of butylated hydroxyanisole is added, and finally citric acid is added to adjust the pH value to 5.0 to obtain the product.

[0074] Comparative Example 7: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0075] Pittosporum tobira bark 30g, Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0076] (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0077] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0078] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0079] (4) Add 0.2% of the total weight of sodium benzoate to step (3), and finally add citric acid to adjust the pH value to 5.0.

[0080] Comparative Example 8: A pharmaceutical preparation for preventing and treating poultry adenovirus, the content and preparation process are as follows:

[0081] Pittosporum tobira bark 30g, Artemisia capillaris 30g, Isatis root 20g, Honeysuckle 10g, Cuscuta australis 10g, Eclipta prostrata 8g, Citrus aurantium 8g, Polygonum multiflorum 6g.

[0082] (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use;

[0083] (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate;

[0084] (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid;

[0085] (4) Add 0.05% of the total weight of butylated hydroxyanisole to step (3), and finally add hydrochloric acid to adjust the pH value to 5.0 to obtain the product.

[0086] 1. Pharmacodynamics Experiment of the Drug of the Present Invention

[0087] 1.1 Adenovirus type I infection chicken model

[0088] Five white-feathered broiler chickens diagnosed with adenovirus type I were placed into a test flock of 150 3-week-old white-feathered broiler chickens for co-breeding and infection testing. After 5 days, the changes in the flock were observed and the test chickens were selected according to the scores in Table 1:

[0089] Table 1 Score classification and symptom statistics

[0090]

[0091] Note: Chickens with a total score of 3 or less are diagnosed as being infected with adenovirus type 1.

[0092] 1.2 Grouping and Dosing

[0093] The type I adenovirus model chickens prepared in 1.1 were randomly divided into 8 groups, namely, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group and model group, with 10 chickens in each group, and the oral solution prepared in the corresponding example was administered, 0.2 mL per chicken, twice a day, for 7 consecutive days. Ten healthy chickens (white broilers) were selected as blank controls, and the blank group and model group were gavage-administered with the same dose of 0.9% normal saline.

[0094] 1.3 Testing

[0095] 1.3.1 Check initial weight, first week weight, and second week weight

[0096] Body weight measurement is an important indicator for evaluating treatment efficacy and animal health. Body weight reflects health status. Weigh each animal in each group at the end of the first and second weeks of treatment.

[0097] 1.3.2 Points Improvement

[0098] There was no significant difference in the scores between the experimental groups after modeling, and the scores ranged from 0 to 3. After 1 week of treatment, the scores were finally calculated.

[0099] 1.4 Statistical Analysis

[0100] Graphad Prism 9.0 was used for statistical analysis, and the data were expressed as mean ± standard deviation ( ±S). Data from each group were tested for normal distribution and homogeneity of variance, meeting the requirements of the Pearson test. Intergroup comparisons were performed using one-way analysis of variance. P < 0.05 was considered statistically significant.

[0101] 1.5 Results Analysis

[0102] 1.5.1 Analysis of body weight in the first week and the second week, see Table 2 and Figure 1

[0103] Table 2 Analysis of body weight in the first week and the second week of the relevant examples

[0104]

[0105] Figure 1 It can be seen that the effects of the pharmaceutical preparations in different examples on body weight vary greatly. At week 0, there was no significant difference between the examples (P>0.05). The body weight test at week 1 showed that there were significant differences between Examples 1-3 and Comparative Examples 1-4 (P<0.05). The inventors believe that the removal of the Pittosporum tobira bark or changes in dosage or substitution may affect the therapeutic effect of the entire composition. This difference was more obvious at week 2, and the growth rate of the white-feathered chickens in Comparative Examples 1-4 was significantly reduced.

[0106] 1.5.2 Points improvement, see Table 3 and Figure 2

[0107] Table 3: Improvement of points in relevant embodiments

[0108]

[0109] Figure 2 It can be seen that at the end of the first week after treatment, the scores of each example group increased, indicating the therapeutic effect of the pharmaceutical preparation on adenovirus. A comparison of Examples 1-3 and Comparative Examples 1-4 showed that the scores of Comparative Examples 1-4 were significantly lower than those of Examples 1-3, and this significant feature was highly significant (P<0.0001).

[0110] 2. Determination of chlorogenic acid content

[0111] [Content Determination] Chlorogenic acid was determined according to the high performance liquid chromatography method (General Rule 0512).

[0112] Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel was used as the filler; acetonitrile-0.05% phosphoric acid solution (10:90) was used as the mobile phase; and the detection wavelength was 327 nm.

[0113] Preparation of reference solution: Take an appropriate amount of chlorogenic acid reference substance, accurately weigh it, place it in a brown volumetric flask, and add 50% methanol to make a solution containing 40 μg per 1 mL.

[0114] Preparation of test solution: Take 0.3 mL of solution of Example 1, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Comparative Example 8, place in a 50 mL brown volumetric flask, add 50% methanol to the scale, shake well, centrifuge, accurately measure 3 mL of supernatant, place in a 10 mL brown volumetric flask, add 50% methanol to the scale, shake well, and obtain.

[0115] Determination method: Accurately aspirate 15 μL of reference solution and test solution respectively, inject into liquid chromatograph, and determine.

[0116] Accelerated test: Store at 40℃±2℃, 75%±5% relative humidity (RH), and test the chlorogenic acid content at 0, 1, 2, 3, and 6 months. Figure 3 and as shown in Table 4.

[0117] Table 4 Chlorogenic acid content in related examples

[0118]

[0119] from Figure 3 As can be seen, the chlorogenic acid content of the examples is above 45 mg / mL. Comparative Examples 7-8 showed a significant decrease in chlorogenic acid content during storage. The inventors believe this may be due to the different preservatives used, which reacted with the chlorogenic acid in the oral solution, leading to a decrease in the active chlorogenic acid substance. Alternatively, the buffer system formed by the preservatives may not be adapted to the storage environment of chlorogenic acid, leading to a decrease in chlorogenic acid. Comparative Examples 5-6, which used different extraction processes, showed lower chlorogenic acid content at month 0.

Claims

1. A traditional Chinese medicine composition for preventing and treating poultry adenovirus, the traditional Chinese medicine composition being prepared from the following raw materials in parts by weight: 23-37 parts of Pittosporum tobira bark, 23-37 parts of Artemisia capillaris, 15-25 parts of Isatis indigotica, 5-15 parts of Honeysuckle, 5-15 parts of Cuscuta australis, 5-11 parts of Eclipta prostrata, 5-11 parts of Citrus aurantium, and 3-9 parts of Polygonum multiflorum.

2. The Chinese medicinal composition for preventing and treating poultry adenovirus according to claim 1, wherein: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 parts of Pittosporum tobira bark, 30 parts of Artemisia capillaris, 20 parts of Radix Isatidis, 10 parts of Flos Lonicerae, 10 parts of Cuscuta australis, 8 parts of Ecliptae, 8 parts of Fructus Aurantii Immaturus and 6 parts of Caulis Polygoni Multiflori.

3. The Chinese medicinal composition for preventing and treating poultry adenovirus according to claim 1, wherein: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 23 parts of Pittosporum tobira bark, 23 parts of Artemisia capillaris, 15 parts of Radix Isatidis, 5 parts of Flos Lonicerae, 5 parts of Cuscuta australis, 5 parts of Ecliptae, 5 parts of Fructus Aurantii Immaturus and 3 parts of Caulis Polygoni Multiflori.

4. The Chinese medicinal composition for preventing and treating poultry adenovirus according to claim 1, wherein: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 37 parts of Pittosporum tobira bark, 37 parts of Artemisia capillaris, 25 parts of Radix Isatidis, 15 parts of Flos Lonicerae, 15 parts of Cuscuta australis, 11 parts of Eclipta prostrata, 11 parts of Fructus Aurantii Immaturus and 9 parts of Caulis Polygoni Multiflori.

5. A pharmaceutical preparation for preventing and treating poultry adenovirus, characterized in that: The pharmaceutical preparation is composed of the traditional Chinese medicine composition according to any one of claims 1 to 4 and pharmaceutically acceptable excipients.

6. The pharmaceutical preparation for preventing and treating poultry adenovirus according to claim 5, characterized in that The pharmaceutical preparation is selected from any one of powder, oral liquid or granule.

7. The pharmaceutical preparation for preventing and treating poultry adenovirus according to claim 6, characterized in that: The pharmaceutical preparation is selected from oral liquid.

8. The pharmaceutical preparation for preventing and treating poultry adenovirus according to claim 7, characterized in that: The preparation method of the oral liquid comprises the following steps: (1) Add 8-15 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 4-8 hours; simmer under steam reflux for 1.5-2.5 hours, filter to obtain a filtrate and a filter residue; add 4-8 times the weight of water to the filter residue, simmer under steam reflux for 0.5-1.5 hours, filter, combine the two filtrates, and use the filter residue for later use; (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue of step (1), add water and boil twice for 0.5-1.5 hours, combine the two decoctions, filter, and obtain a filtrate; (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.02-1.05 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid; (4) Add 0.1-0.3% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 4.5-5.5 to obtain the product.

9. The pharmaceutical preparation for preventing and treating poultry adenovirus according to claim 8, characterized in that: The preparation method of the oral liquid comprises the following steps: (1) Add 13 times the weight of Pittosporum tobira bark, Artemisia capillaris, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to water and soak for 6 hours; steam reflux and boil for 2 hours, filter to obtain a filtrate and a filter residue; add 6 times the weight of water to the filter residue, steam reflux and boil for 1 hour, filter, combine the two filtrates, and use the filter residue for later use; (2) Crush honeysuckle, Eclipta prostrata, and Fructus Aurantii Immaturus, add the mixture to the filter residue from step (1), add water and boil twice for 1 hour, combine the two decoctions, filter, and obtain a filtrate; (3) The filtrate of step (2) is combined with the medicinal liquid distilled from step (1), concentrated to a relative density of 1.03 at 80-90°C, and cooled to room temperature to obtain a Chinese medicine oral liquid; (4) Add 0.2% of the total weight of butylated hydroxyanisole to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

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