Automatic sterile sampling butt joint biochemical analyzer device and method

Abstract

The invention relates to the technical field of biopharmaceutical equipment, and provides an automatic sterile sampling butt joint biochemical analyzer device and method.The automatic sterile sampling butt joint biochemical analyzer device comprises a controller, a sampling pipeline, a CIP pipeline and an SIP pipeline, and the sampling pipeline comprises a sample temporary storage pipe and a sampling hose; one end of the sampling hose is communicated with the sample temporary storage tube, and the other end of the sampling hose is communicated with an external biochemical analyzer; the CIP pipeline comprises a CIP liquid inlet pipe and a CIP liquid return pipe, one end of the CIP liquid inlet pipe is communicated with external cleaning liquid output equipment, the other end of the CIP liquid inlet pipe is communicated with the output end of the first valve, one end of the CIP liquid return pipe is communicated with the top end of the sample temporary storage pipe, and the other end of the CIP liquid return pipe is communicated with an external CIP liquid return collecting device; the SIP pipeline comprises an SIP air inlet pipe and an SIP air return pipe. According to the automatic sterile sampling butt joint biochemical analyzer device, the sampling efficiency can be improved, and meanwhile, the risk that a sample is contaminated by bacteria is reduced.

Description

Technical Field

[0001] This application relates to the technical field of biopharmaceutical equipment, and particularly to an automated aseptic sampling and docking biochemical analyzer device and method. Background Art

[0002] In the biopharmaceutical industry, bioreactors are widely used. They can be used to produce antibiotics, enzymes, proteins, and other biological products, and can also be used to treat wastewater and solid waste, etc. During the biopharmaceutical process, it is necessary to sample the liquid in the bioreactor (mainly reactants and organisms).

[0003] During the sampling process, in order to prevent microorganisms from contaminating the bioreactor and the sampling environment, aseptic sampling is generally used. The usual operation is to first sterilize the sampling bottle in a sterilizer, connect the sampling pipeline, then disinfect the sampling pipeline with steam, and then perform sampling. After sampling, the sampling bottle is then connected to the biochemical analyzer equipment, and finally biochemical analysis is carried out. However, in such a sampling process, the entire process is cumbersome, time-consuming, and there is a risk of sample contamination by bacteria. Therefore, a device that can automatically sample and automatically send the sample to a biochemical analyzer for analysis is needed, and it can automatically perform CIP / SIP before and after sampling to ensure that the sample is not contaminated by bacteria. Summary of the Invention

[0004] In order to improve the sampling efficiency and reduce the risk of sample contamination by bacteria, this application provides an automated aseptic sampling and docking biochemical analyzer device and method.

[0005] In a first aspect, an automated aseptic sampling and docking biochemical analyzer device provided by this application adopts the following technical solution: An automated aseptic sampling and docking biochemical analyzer device includes a controller, a sampling pipeline, a CIP pipeline, and a SIP pipeline. The sampling pipeline includes a sample storage tube and a sampling hose that are connected in sequence. The sample storage tube is connected to the bioreactor and a first valve is provided between them. One end of the sampling hose is connected to the bottom end of the sample storage tube through a second valve, and the other end is connected to an external biochemical analyzer. The CIP pipeline includes a CIP inlet pipe and a CIP return pipe. One end of the CIP inlet pipe is connected to an external cleaning liquid output device, and the other end is connected to the output end of the first valve through a third valve. One end of the CIP return pipe is connected to the top end of the sample storage tube, and the other end is connected to an external CIP return liquid collection device through a fourth valve. The SIP pipeline includes a SIP inlet pipe and a SIP return pipe. One end of the SIP inlet pipe is connected to an external steam generator, and the other end is connected to the CIP inlet pipe. One end of the SIP return pipe is connected to the top end of the sample storage tube, and the other end is connected to an external condensed water recovery device through a fifth valve. Each valve is connected to the controller for control.

[0006] By adopting the above technical solution, through the arrangement of the sampling pipeline, CIP pipeline and SIP pipeline, the third valve and the fourth valve are opened and the first valve, the second valve and the fifth valve are closed, and the cleaning liquid is introduced by the cleaning liquid output device to clean the pipeline; after the cleaning is completed, the third valve and the fifth valve are opened and the first valve, the second valve and the fourth valve are closed, and steam is introduced by the steam generator to sterilize the pipeline; after the sterilization is completed, the first valve and the fifth valve are opened and the third valve and the fourth valve are closed, so that the liquid in the bioreactor enters the sample temporary storage tube through the first valve. When it reaches the set height, the first valve is shut down and the second valve is opened, and the biochemical analyzer can obtain the sample to be analyzed output by the bioreactor from the sample temporary storage tube; the CIP pipeline and the SIP pipeline cooperate to clean and sterilize the sample temporary storage tube to ensure that the sample is not contaminated during subsequent sampling. The whole process can be completed by the controller controlling the coordinated action of each valve without manual participation, and the sampling efficiency is high.

[0007] Optionally, the sample temporary storage tube is vertically arranged, the bottom end of the sample temporary storage tube is connected with a sampling tube, the end of the sampling tube far away from the sample temporary storage tube is communicated with the bioreactor, the sampling tube is obliquely arranged, the first valve is arranged on the sampling tube, and the communication point of the sampling tube and the bioreactor is not higher than the lowest liquid level of the liquid in the bioreactor; a flow regulating valve is connected to the bottom end of the sample temporary storage tube.

[0008] By adopting the above technical solution, the vertically arranged sample temporary storage tube is more conducive to the storage of the sample. At the same time, by controlling the opening time of the first valve, the amount of the liquid entering the sample temporary storage tube can be controlled to prevent waste of the liquid caused by excessive sampling. The setting position of the sampling tube ensures that there is a positive pressure between the input end and the output end of the first valve, which is convenient for the liquid to enter the sample temporary storage tube and is beneficial to improving the sampling efficiency.

[0009] Optionally, the SIP return air pipe is successively provided with a temperature sensor for detecting the temperature in the pipeline and a steam trap for preventing steam from discharging, the temperature sensor and the steam trap are both located between the fifth valve and the external condensate recovery device, and the controller is in signal connection with the temperature sensor.

[0010] By adopting the above technical solution, the steam trap is used to discharge the condensate and block the steam from discharging to maintain the sterilization pressure in the pipeline. The function of the temperature sensor is to control the sterilization temperature. The temperature sensor can be associated with the SIP steam inlet valve to control the steam intake to ensure thorough sterilization.

[0011] Optionally, the first valve, the second valve, the third valve, the fourth valve and the fifth valve all adopt pneumatic diaphragm valves, and the flow regulating valve adopts a manual diaphragm valve.

[0012] Optionally, an installation branch pipe is connected to the peripheral wall at the bottom end of the sample temporary storage tube, one end of the sampling hose away from the biochemical analyzer is connected to the installation branch pipe, and the sampling hose is connected to the sample temporary storage tube through the installation branch pipe; a connection assembly is provided between the installation branch pipe and the sampling hose, and the sampling hose is detachably installed on the installation branch pipe through the connection assembly.

[0013] By adopting the above technical solution, through the setting of the connection assembly, the sampling hose is detachably installed on the sample temporary storage tube. After the sampling hose is used multiple times, it can be disassembled through the connection assembly for off-line sterilization.

[0014] Optionally, the connection assembly includes a connection joint, a docking ring and a docking sleeve. The connection joint includes a first connection part and a second connection part integrally formed. One end of the sampling hose is sleeved on the outer peripheral wall of the first connection part. The outer diameter of the sampling hose is smaller than the outer diameter of the second connection part. One end of the installation branch pipe is inserted into the second connection part; the docking ring is arranged on the outer peripheral wall of the installation branch pipe, and the outer diameter of the docking ring is adapted to the outer diameter of the second connection part; the docking sleeve includes a first docking part and a second docking part integrally formed. The first docking part is sleeved on the outer peripheral wall of the sampling hose, and the second docking part is simultaneously sleeved on the outer peripheral walls of the second connection part and the docking ring and is threadedly connected to the docking ring.

[0015] By adopting the above technical solution, through the setting of the connection joint, the docking ring and the docking sleeve, when installing the sampling hose, the sampling hose is sleeved on the outer peripheral wall of the first connection part, then the second connection part is aligned with one end of the installation branch pipe and pushed, so that one end of the installation branch pipe is inserted into the second connection part. Then, the docking sleeve is slid, so that the second connection part is docked with the docking ring and the docking sleeve is rotated. After the second connection part is tightened, the sampling hose, the connection joint and the installation branch pipe are connected into one body, improving the disassembly and assembly convenience of the overall structure.

[0016] Optionally, a first sealing ring groove is formed on the outer peripheral wall of the first connection part, and a first sealing ring is arranged in the first sealing ring groove; the first sealing ring groove has a guiding surface, and the guiding surface has an outer diameter gradually increasing from the side close to the second connection part to the side away from the second connection part; a pushing ring is slidably installed in the first sealing ring groove, and the pushing ring is located on the side of the first sealing ring close to the second connection part; the second connection part is provided with a driving component. When the second docking part is screwed to the docking ring, the driving component drives the pushing ring to move away from the second connection part, so that the first sealing ring abuts against the inner peripheral wall of the sampling hose.

[0017] By adopting the above technical solutions, through the arrangement of the first sealing ring, the guiding surface and the pushing ring, when the first sealing ring is installed, the first sealing ring is sleeved into the first sealing ring groove and the first sealing ring is displaced to the side of the first sealing ring groove close to the second connecting part, so that the first sealing ring is completely embedded in the first sealing ring groove. The fact that the first sealing ring is completely embedded in the first sealing ring groove under normal conditions can facilitate the sampling hose to be sleeved on the outer peripheral wall of the first connecting part, avoid the exposure of the first sealing ring from hindering the sleeving of the sampling hose, and further improve the disassembly and assembly convenience between the sampling hose and the first connecting part. After the sampling hose is sleeved on the first connecting part and the installation branch pipe is inserted into the second connecting part, the second docking part is forced to screw the docking ring tightly. At this time, the driving assembly can drive the pushing ring to displace towards the side away from the second connecting part to squeeze the first sealing ring, so that the first sealing ring expands under the action of the guiding surface and is partially exposed from the first sealing ring groove to abut against the inner peripheral wall of the sampling hose, improving the sealing effect between the sampling hose and the first connecting part. After the first sealing ring abuts against the inner peripheral wall of the sampling hose, the first sealing ring and the first docking part form clamping and fixing of the sampling hose, improving the disassembly and assembly convenience of the sampling hose.

[0018] Optionally, a rotating arc groove is formed in the first connecting part. One end of the rotating arc groove penetrates through the guiding surface, and the other end penetrates through the outer peripheral wall of the first connecting part. A pushing arc bar is slidably arranged in the rotating arc groove. When the pushing ring displaces towards the side away from the second connecting part, the pushing ring pushes the pushing arc bar, so that one end of the pushing arc bar away from the pushing ring abuts against the inner peripheral wall of the sampling hose and has a driving force for the sampling hose to displace towards the side close to the second connecting part.

[0019] By adopting the above technical solutions, through the arrangement of the pushing arc bar, when the second docking part screws the docking ring tightly, the pushing ring pushes the first sealing ring to move along the guiding surface towards the side away from the second connecting part and makes the first sealing ring abut against the inner peripheral wall of the sampling hose, resulting in the sampling hose being subjected to a squeezing force for retraction due to the extrusion from the first sealing ring; while the pushing ring pushes the first sealing ring, the pushing ring can push the pushing arc bar, forcing the pushing arc bar to rotate around its own central axis, so that one end of the pushing arc bar can abut against the sampling hose and form a driving force for driving the sampling hose to displace towards the side close to the second connecting part. This driving force cancels out the squeezing force received by the sampling hose, thereby reducing the possibility of the sampling hose retracting when the first sealing ring abuts against the sampling hose, and further improving the connection stability between the sampling hose and the first connecting part.

[0020] Optionally, a flow splitting ring is provided at one end of the first connecting portion away from the second connecting portion, and the inner diameter of the flow splitting ring is smaller than the inner diameter of the first connecting portion; a flow splitting channel is formed between the flow splitting ring and the first connecting portion, the flow splitting channel is arranged in a ring shape around the central axis of the flow splitting ring, and the diameter of the flow splitting channel gradually increases from the side close to the second connecting portion to the side away from the second connecting portion.

[0021] By adopting the above technical solution, through the arrangement of the flow splitting ring and the flow splitting channel, a flow splitting ring is added at one end of the first connecting portion away from the second connecting portion to form a flow splitting channel for the feed liquid to flow through. After the feed liquid enters the flow splitting channel and is discharged out from the outlet end of the flow splitting channel, the feed liquid located at the end face position of the first connecting portion away from the second connecting portion is pushed, so that the feed liquid in the sampling hose is not easily introduced into the gap between the outer peripheral wall of the first connecting portion and the inner peripheral wall of the sampling hose, thereby improving the sealing performance of the overall structure.

[0022] In a second aspect, an automated aseptic sampling method provided by the present application adopts the following technical solution: An automated aseptic sampling method specifically includes: keeping the flow regulating valve in an open state; an automatic CIP step of controlling the opening of the third valve and the fourth valve, closing the first valve, the second valve and the fifth valve, and introducing a cleaning liquid from an external cleaning liquid output device to clean the pipeline, and controlling the closing of the third valve and the fourth valve after a set duration; an automatic SIP step of controlling the opening of the third valve and the fifth valve, closing the first valve, the second valve and the fourth valve, introducing steam from an external steam generator to sterilize the pipeline, obtaining the data of the temperature sensor, and controlling the closing of the third valve and the fifth valve when it reaches the set value and lasts for a set duration; an automatic sampling step of controlling the opening of the first valve and the fifth valve, closing the third valve and the fourth valve, allowing the feed liquid to enter the sample temporary storage tube through the first valve and the flow regulating valve, closing the first valve when it rises to the set height, and then controlling the opening of the second valve, and automatically extracting the feed liquid sample in the sample temporary storage tube by using a peristaltic pump in an external biochemical analyzer to complete the sampling.

[0023] In summary, the present application includes at least one of the following beneficial technical effects: 1. Through the settings of the sampling pipeline, CIP pipeline and SIP pipeline, open the third valve and the fourth valve and close the first valve, the second valve and the fifth valve, and introduce cleaning liquid from the cleaning liquid output device to clean the pipeline; after the cleaning is completed, open the third valve and the fifth valve and close the first valve, the second valve and the fourth valve, and introduce steam from the steam generator to sterilize the pipeline; after the sterilization is completed, open the first valve and the fifth valve and close the third valve and the fourth valve, so that the liquid in the bioreactor enters the sample temporary storage tube through the first valve. When it reaches the set height, close the first valve and open the second valve, and the biochemical analyzer can obtain the sample to be analyzed output by the bioreactor from the sample temporary storage tube; the CIP pipeline and the SIP pipeline cooperate to clean and sterilize the sample temporary storage tube to ensure that the sample is not contaminated during subsequent sampling. The entire process can be completed by the controller controlling the coordinated actions of each valve without manual participation, and the sampling efficiency is high; 2. Through the settings of the first sealing ring, the guiding surface and the pushing ring, when the first sealing ring is installed, the first sealing ring is sleeved into the first sealing ring groove and the first sealing ring is displaced to the side of the first sealing ring groove close to the second connecting part, so that the first sealing ring is completely embedded in the first sealing ring groove. The first sealing ring being completely embedded in the first sealing ring groove under normal conditions can facilitate the sampling hose to be sleeved on the outer peripheral wall of the first connecting part, avoid the exposure of the first sealing ring from hindering the sleeving of the sampling hose, and thus improve the disassembly and assembly convenience between the sampling hose and the first connecting part. After the sampling hose is sleeved on the first connecting part and the installation branch pipe is inserted into the second connecting part, the second docking part is forced to tighten the docking ring. At this time, the driving component can drive the pushing ring to displace away from the second connecting part to squeeze the first sealing ring, so that the first sealing ring expands under the action of the guiding surface and is partially exposed outside the first sealing ring groove to abut against the inner peripheral wall of the sampling hose, improving the sealing effect between the sampling hose and the first connecting part. After the first sealing ring abuts against the inner peripheral wall of the sampling hose, the first sealing ring and the first docking part form a clamping and fixing of the sampling hose, improving the disassembly and assembly convenience of the sampling hose; 3. Through the setting of the pushing arc strip, when the second docking part tightens the docking ring, the pushing ring pushes the first sealing ring to move away from the second connecting part along the guiding surface and makes the first sealing ring abut against the inner peripheral wall of the sampling hose, resulting in the sampling hose being subjected to a squeezing force of retreat due to the extrusion from the first sealing ring; while the pushing ring pushes the first sealing ring, the pushing ring can push the pushing arc strip, forcing the pushing arc strip to rotate around its own central axis, so that one end of the pushing arc strip can abut against the sampling hose and form a driving force to drive the sampling hose to displace towards the second connecting part. This driving force cancels out the squeezing force received by the sampling hose, thereby reducing the possibility of the sampling hose retreating when the first sealing ring abuts against the sampling hose, and thus improving the connection stability between the sampling hose and the first connecting part. Description of the Drawings

[0024] Figure 1 is the overall structural schematic diagram of Embodiment 1; Figure 2 is the structural schematic diagram showing the sampling pipeline, CIP pipeline and SIP pipeline in Embodiment 1; Figure 3 is the partial cross-sectional view showing the connection component in Embodiment 1; Figure 4 is the partial cross-sectional view showing the connection component in Embodiment 2; Figure 5 is Figure 4 the enlarged view at A in Figure 6 is the partial cross-sectional view showing the pushing arc bar in Embodiment 3; Figure 7 is the partial cross-sectional view showing the shunt channel in Embodiment 4.

[0025] Explanation of reference numerals: 1, sampling pipeline; 11, sample temporary storage tube; 111, first valve; 112, second valve; 113, sampling tube; 114, flow regulating valve; 12, sampling hose; 13, installation branch pipe; 131, third sealing ring groove; 2, CIP pipeline; 21, CIP liquid inlet pipe; 211, third valve; 22, CIP liquid return pipe; 221, fourth valve; 3, SIP pipeline; 31, SIP air inlet pipe; 32, SIP air return pipe; 321, fifth valve; 322, temperature sensor; 323, steam trap; 4, bioreactor; 5, biochemical analyzer; 6, cleaning liquid output device; 61, CIP liquid return collection device; 7, steam generator; 71, condensate recovery device; 8, connection component; 81, connection joint; 811, first connection part; 8111, first sealing ring groove; 8112, guiding surface; 8113, rotating arc groove; 812, second connection part; 8121, second sealing ring groove; 8122, installation ring groove; 813, first sealing ring; 814, pushing ring; 815, pushing arc bar; 816, shunt ring; 8161, shunt channel; 8162, countersunk head bolt; 8163, isolation ball; 817, pushing piece; 82, docking ring; 83, docking sleeve; 831, first docking part; 832, second docking part; 84, tower joint; 841, inserting part; 842, rotating part; 843, sleeving part; 844, third sealing ring; 85, locking part; 9, driving component; 91, first driving ring; 911, second sealing ring; 92, first driving rod; 93, second driving ring; 94, second driving rod; 95, return spring. Detailed implementation manners

[0026] The following further elaborates on this application in conjunction with the Figure 1 - Figure 7 accompanying drawings.

[0027] Example 1: This embodiment of the present application discloses an automated aseptic sampling and docking biochemical analyzer device, which is mainly applied to the pharmaceutical industry for highly aseptic sampling of the liquid in a bioreactor 4, especially in a fermentation tank.

[0028] Referring to Figure 1 、 Figure 2 An automated aseptic sampling and docking biochemical analyzer device includes a controller, a sampling pipeline 1, a CIP pipeline 2, and an SIP pipeline 3. Among them, the sampling pipeline 1 includes a sample temporary storage tube 11 and a sampling hose 12. The sample temporary storage tube 11 and the sampling hose 12 are connected in sequence. The sample temporary storage tube 11 is connected to the wall of the bioreactor 4, and a first valve 111 is installed between them; the sample temporary storage tube 11 is arranged vertically. One end of the sampling hose 12 is connected to the bottom end of the sample temporary storage tube 11 through a second valve 112, and the other end is connected to an external biochemical analyzer 5.

[0029] Referring to Figure 1 、 Figure 2 The bottom end of the sample temporary storage tube 11 is connected with a sampling tube 113. One end of the sampling tube 113 is connected to the sample temporary storage tube 11, and the other end is connected to the wall of the bioreactor 4 and communicates with the inside of the bioreactor 4; the sampling tube 113 is arranged obliquely, and the height of the sampling tube 113 gradually decreases from the end close to the sample temporary storage tube 11 to the end far from the sample temporary storage tube 11. The first valve 111 is installed on the sampling tube 113, and the connection point of the sampling tube 113 and the bioreactor 4 is not higher than the lowest liquid level of the liquid in the bioreactor 4.

[0030] In the specific sampling process, the inside of the tank body of the bioreactor 4 can be set to a positive air pressure. When the first valve 111 is opened, the liquid will automatically enter the sample temporary storage tube 11. By controlling the opening time of the first valve 111, the amount of liquid entering the sample temporary storage tube 11 can be controlled to prevent waste of liquid caused by excessive sampling; then, the peristaltic pump in the external biochemical analyzer 5 is used to suck the liquid in the sample temporary storage tube 11 for analysis to achieve sampling.

[0031] Referring to Figure 1 、 Figure 2, a flow regulating valve 114 is connected to the bottom end of the sample temporary storage tube 11. When the docking device is in an idle state, the sampling pipeline 1 can be shut off, and the flow rate can be controlled during the use of the device. The amount of the liquid material entering the sample temporary storage tube 11 can be controlled by the opening time of the first valve 111. When sampling for the first time, it is necessary to ensure the amount of the liquid material entering the sample temporary storage tube 11 through the flow regulating valve 114 to prevent overfilling. In practical applications, adjustment is required during the first sampling, and for subsequent samplings, the initial state of the flow regulating valve 114 can be maintained. Preferably, the first valve 111 and the flow regulating valve 114 adopt a combined valve structure to avoid dead corners in cleaning and sterilization.

[0032] Refer to Figure 1 , Figure 2 , the CIP pipeline 2 includes a CIP inlet pipe 21 and a CIP return pipe 22. One end of the CIP inlet pipe 21 is connected to an external cleaning liquid output device 6, and the other end is connected to the output end of the first valve 111 through a third valve 211. A CIP inlet valve (not shown in the figure) is arranged between the external cleaning liquid output device 6 and the CIP inlet pipe 21; one end of the CIP return pipe 22 is connected to the top end of the sample temporary storage tube 11, and the other end is connected to an external CIP return liquid collection device 61 through a fourth valve 221.

[0033] In this embodiment, the connections between the above pipelines all adopt a smooth integrated connection method to avoid dead corners in the flow of the liquid material. Through the above solution, when the pipelines need to be cleaned, only the first valve 111 and the second valve 112 need to be shut off, and the third valve 211 and the fourth valve 221 are opened, and then the pipelines can be flushed and cleaned with the cleaning liquid.

[0034] Refer to Figure 1 , Figure 2 , the SIP pipeline 3 includes a SIP inlet pipe 31 and a SIP return pipe 32. In the implementation mode of the present application, the sterilization operation of the cleaned pipelines is mainly carried out by using high-temperature steam. Therefore, one end of the SIP inlet pipe 31 is connected to an external steam generator 7, and the other end is connected to the CIP inlet pipe 21. A SIP inlet steam valve (not shown in the figure) is arranged between the external steam generator 7 and the SIP inlet pipe 31; one end of the SIP return pipe 32 is connected to the top end of the sample temporary storage tube 11, and the other end is connected to an external condensed water recovery device 71 through a fifth valve 321.

[0035] In this embodiment, when sterilization operation needs to be performed on the pipeline, only the first valve 111, the second valve 112 and the fourth valve 221 need to be shut off, and the third valve 211 and the fifth valve 321 need to be opened, so that the high-temperature steam from the outside can be introduced into the sample storage tube 11 through the SIP inlet pipe 31, and then discharged through the SIP return pipe 32, realizing the sterilization of the pipeline. In order to simplify the pipeline structure, the above-mentioned SIP inlet pipe 31 and the CIP inlet pipe 21 are multiplexed in a time-sharing manner.

[0036] Referring to Figure 1 、 Figure 2 , in this embodiment, the controller (not shown in the figure) adopts a PLC or a single-chip microcomputer control module. The first valve 111, the second valve 112, the third valve 211, the fourth valve 221 and the fifth valve 321 all adopt pneumatic diaphragm valves, and each pneumatic diaphragm valve is connected to the controller for control. The flow regulating valve 114 adopts a manual diaphragm valve.

[0037] Referring to Figure 1 、 Figure 2 , in order to ensure the sterilization effect of the pipeline, a temperature sensor 322 and a steam trap 323 are sequentially installed on the SIP return pipe 32 along the exhaust direction. The temperature sensor 322 is used to detect the temperature inside the pipeline, and the steam trap 323 is used to prevent steam from being discharged. The temperature sensor 322 and the steam trap 323 are both located between the fifth valve 321 and the external condensate recovery device 71.

[0038] The controller is signal-connected to the temperature sensor 322. The temperature sensor 322 collects the steam temperature in the SIP return pipe 32, and the controller receives and responds to the above temperature data to control the steam intake. Preferably, the temperature in the above pipeline is maintained at 121 °C. The steam trap 323 blocks the steam and discharges the condensate, keeping the pressure inside the pipeline within a set range, preferably greater than 0.1 Mpa. By detecting the temperature in the SIP pipeline 3, it is determined whether the sample storage tube 11 and related valves are qualified for sterilization. After sterilization is completed, the fifth valve 321 and the third valve 211 are shut off by the controller.

[0039] Referring to Figure 1 、 Figure 2 , an installation branch pipe 13 is connected to the peripheral wall at the bottom end of the sample storage tube 11. The installation branch pipe 13 is located on the side of the flow regulating valve 114 away from the first valve 111. One end of the installation branch pipe 13 away from the sample storage tube 11 is used to connect a sampling hose 12. One end of the sampling hose 12 away from the biochemical analyzer 5 is connected to the sample storage tube 11 through the installation branch pipe 13; a connection assembly 8 is arranged between the installation branch pipe 13 and the sampling hose 12, and the sampling hose 12 is detachably installed on the installation branch pipe 13 through the connection assembly 8. In actual application, after the sampling hose 12 is used multiple times, off-line sterilization is required (that is, the sampling hose 12 needs to be detached) to reduce the biological load.

[0040] Reference Figure 2 and Figure 3 In this embodiment, the connection component 8 includes a stepped joint 84 and a locking member 85. The stepped joint 84 includes an insertion portion 841, a rotating portion 842, and a sleeve portion 843 that are integrally formed. The rotating portion 842 is disposed between the insertion portion 841 and the sleeve portion 843. The outer shape of the rotating portion 842 is polygonal for docking tools such as wrenches. The insertion portion 841 is inserted into the sampling hose 12, and the outer diameter of the insertion portion 841 is adapted to the inner diameter of the sampling hose 12. The sleeve portion 843 is sleeved on the outer peripheral wall of the installation branch pipe 13, and a threaded connection (threads are not shown in the figure) is provided between the inner peripheral wall of the sleeve portion 843 and the outer peripheral wall of the installation branch pipe 13. In this embodiment, the locking member 85 is provided as a hose clamp, and the hose clamp is tightened on the outer peripheral wall of the sampling hose 12 to fixedly connect the sampling hose 12 and the insertion portion 841.

[0041] Reference Figure 3 A plurality of embedding annular grooves are formed on the outer peripheral wall of the insertion portion 841. The plurality of embedding annular grooves are arranged at intervals along the central axis of the insertion portion 841, and each embedding annular groove is annularly formed around the central axis of the embedding portion. A third sealing ring 844 is embedded in each embedding annular groove, and a part of the third sealing ring 844 is exposed outside the embedding annular groove to abut against the inner peripheral wall of the sampling hose 12.

[0042] The implementation principle of Embodiment 1 of the present application is as follows: Open the third valve 211 and the fourth valve 221 and close the first valve 111, the second valve 112, and the fifth valve 321. Inject cleaning liquid into the pipeline by the cleaning liquid output device 6 to clean the pipeline. After cleaning, open the third valve 211 and the fifth valve 321 and close the first valve 111, the second valve 112, and the fourth valve 221. Inject steam into the pipeline by the steam generator 7 to sterilize the pipeline. After sterilization, open the first valve 111 and the fifth valve 321 and close the third valve 211 and the fourth valve 221, so that the liquid in the bioreactor 4 enters the sample temporary storage tube 11 through the first valve 111. When it reaches the set height, close the first valve 111 and open the second valve 112. The biochemical analyzer 5 can obtain the sample to be analyzed output by the bioreactor 4 from the sample temporary storage tube 11. The CIP pipeline 2 and the SIP pipeline 3 cooperate to clean and sterilize the sample temporary storage tube 11 to ensure that the sample is not contaminated during subsequent sampling. The entire process can be completed by the controller controlling the coordinated action of each valve, without manual participation, and the sampling efficiency is high.

[0043] Embodiment 2: The embodiment of the present application discloses an automatic aseptic sampling and docking biochemical analyzer device.

[0044] Reference Figure 4 and Figure 5, the difference between the automated aseptic sampling docking biochemical analyzer device disclosed in the embodiment of the present application and Embodiment 1 is as follows: In this embodiment, the connection component 8 includes a connection joint 81, a docking ring 82, and a docking sleeve 83. The connection joint 81 includes a first connection portion 811 and a second connection portion 812 which are integrally formed. The first connection portion 811 and the second connection portion 812 are coaxially arranged. The end of the sampling hose 12 far from the biochemical analyzer 5 is sleeved on the outer peripheral wall of the first connection portion 811. The inner diameter of the sampling hose 12 is adapted to the outer diameter of the first connection portion 811, and the outer diameter of the sampling hose 12 is smaller than the outer diameter of the second connection portion 812. One end of the installation branch pipe 13 far from the sample temporary storage pipe 11 is inserted into the second connection portion 812, and the outer diameter of the installation branch pipe 13 is adapted to the inner diameter of the second connection portion 812.

[0045] Refer to Figure 4 , Figure 5 , the docking ring 82 is fixedly installed on the outer peripheral wall of the installation branch pipe 13, and the docking ring 82 and the installation branch pipe 13 are integrally formed. The outer diameter of the docking ring 82 is adapted to the outer diameter of the second connection portion 812. The docking sleeve 83 includes a first docking portion 831 and a second docking portion 832 which are integrally formed. The first docking portion 831 is sleeved on the outer peripheral wall of the sampling hose 12, and the inner diameter of the first docking portion 831 is adapted to the outer diameter of the sampling hose 12. The second docking portion 832 is simultaneously sleeved on the outer peripheral walls of the second connection portion 812 and the docking ring 82, and the inner diameter of the second docking portion 832 is adapted to the outer diameter of the docking ring 82. The second docking portion 832 is threadedly connected to the docking ring 82.

[0046] Refer to Figure 4 , Figure 5 , a first sealing ring groove 8111 is formed on the outer peripheral wall of the first connection portion 811. The first sealing ring groove 8111 is annularly formed around the central axis of the first connection portion 811, and a first sealing ring 813 is embedded in the first sealing ring groove 8111. The inner wall of the first sealing ring groove 8111 has a guiding surface 8112, and the guiding surface 8112 has an outer diameter that gradually increases from the side close to the second connection portion 812 to the side far from the second connection portion 812.

[0047] Refer to Figure 4 , Figure 5, a pushing ring 814 is slidably installed in the first sealing ring groove 8111. The pushing ring 814 is slidably installed in the first sealing ring groove 8111 and can be displaced along the central axis of the first connecting portion 811. The outer diameter of the pushing ring 814 is not greater than the outer diameter of the first connecting portion 811. The pushing ring 814 is located on the side of the first sealing ring 813 close to the second connecting portion 812. A driving assembly 9 is provided on the second connecting portion 812. When the second docking portion 832 tightens the docking ring 82, the driving assembly 9 drives the pushing ring 814 to displace away from the second connecting portion 812, so that the first sealing ring 813 abuts against the inner peripheral wall of the sampling hose 12.

[0048] Refer to Figure 4 , Figure 5 , a second sealing ring groove 8121 is formed in the inner wall of the second connecting portion 812. The second sealing ring groove 8121 is annularly arranged around the central axis of the second connecting portion 812. The driving assembly 9 includes a first driving ring 91, a first driving rod 92 and a driving member. The first driving ring 91 is slidably installed in the second sealing ring groove 8121 and can be displaced along the central axis of the second connecting portion 812. There are a plurality of first driving rods 92. The plurality of first driving rods 92 are spaced apart around the central axis of the second connecting portion 812. One end of each first driving rod 92 is fixedly installed on the side wall of the first driving ring 91, and the other end penetrates into the first sealing ring groove 8111 and is fixedly connected to the pushing ring 814. The pushing ring 814 and the first driving ring 91 are connected by a plurality of first driving rods 92.

[0049] Refer to Figure 4 , Figure 5 , a second sealing ring 911 is fixedly installed on the side wall of the first driving ring 91 away from the pushing ring 814. The second sealing ring 911 is partially exposed from the second sealing ring groove 8121. An installation ring groove 8122 is formed in the inner peripheral wall of the second connecting portion 812. The installation ring groove 8122 is annularly arranged around the central axis of the second connecting portion 812. The driving member includes a second driving ring 93, a second driving rod 94 and a return spring 95. The second driving ring 93 is slidably installed in the installation ring groove 8122 and can be displaced along the central axis of the second connecting portion 812. The inner diameter of the second driving ring 93 is smaller than the inner diameter of the second connecting portion 812. A third sealing ring groove 131 for embedding the second sealing ring 911 is formed in the end face of the installation branch pipe 13. When the installation branch pipe 13 is inserted into the second connecting portion 812, the second driving ring 93 is embedded into the third sealing ring groove 131 to squeeze the second sealing ring 911.

[0050] Refer to Figure 4 , Figure 5, A plurality of second drive rods 94 are provided. The plurality of second drive rods 94 are arranged at intervals around the central axis of the second connection portion 812. One end of each second drive rod 94 is fixedly connected to the side wall of the second drive ring 93, and the other end extends to the side of the second connection portion 812 away from the first connection portion 811 for the docking ring 82 to abut. When the second docking portion 832 tightens the docking ring 82, the docking ring 82 pushes the second drive rod 94, so that the first drive ring 91 and the second drive ring 93 clamp the second sealing ring 911 at the same time, and the push ring 814 pushes the first sealing ring 813 to move along the guide surface 8112 away from the second connection portion 812 to abut against the inner peripheral wall of the sampling hose 12.

[0051] The return spring 95 is installed in the installation ring groove 8122. One end of the return spring 95 is fixedly connected to the inner wall of the installation ring groove 8122, and the other end is fixedly connected to the side wall of the second drive ring 93. In the normal state of the return spring 95, one end of the second drive rod 94 extends out of the second connection portion 812 for the docking ring 82 to abut.

[0052] The implementation principle of Embodiment 2 of the present application is as follows: When the first sealing ring 813 is installed, the first sealing ring 813 is sleeved into the first sealing ring groove 8111 and the first sealing ring 813 is displaced to the side of the first sealing ring groove 8111 close to the second connection portion 812, so that the first sealing ring 813 is completely embedded in the first sealing ring groove 8111. The fact that the first sealing ring 813 is completely embedded in the first sealing ring groove 8111 under normal conditions can facilitate the sampling hose 12 to be sleeved on the outer peripheral wall of the first connection portion 811, avoid the exposure of the first sealing ring 813 from hindering the sleeving of the sampling hose 12, and thus improve the disassembly and assembly convenience between the sampling hose 12 and the first connection portion 811.

[0053] After the sampling hose 12 is sleeved on the first connection portion 811 and the installation branch pipe 13 is inserted into the second connection portion 812, the second docking portion 832 is forced to tighten the docking ring 82. At this time, the docking ring 82 can push the second drive rod 94, so that the first drive ring 91 and the second drive ring 93 clamp the second sealing ring 911 at the same time, and the push ring 814 pushes the first sealing ring 813 to move along the guide surface 8112 away from the second connection portion 812 to abut against the inner peripheral wall of the sampling hose 12, greatly improving the sealing effect of the overall structure and the disassembly and assembly convenience of the overall structure.

[0054] Embodiment 3: The embodiment of the present application discloses an automated aseptic sampling docking biochemical analyzer device.

[0055] Refer to Figure 6 , The difference between the automated aseptic sampling docking biochemical analyzer device disclosed in the embodiment of the present application and Embodiment 2 is: In this embodiment, a rotating arc groove 8113 is formed in the first connecting portion 811. The number of the rotating arc grooves 8113 is multiple, and the multiple rotating arc grooves 8113 are arranged at intervals around the central axis of the first connecting portion 811. One end of the rotating arc groove 8113 penetrates the guiding surface 8112, and the other end penetrates the outer peripheral wall of the first connecting portion 811; a pushing arc bar 815 is slidably installed in each rotating arc groove 8113. One end of the pushing arc bar 815 close to the second connecting portion 812 extends out of the rotating arc groove 8113 and is connected with a pushing piece 817 located in the first sealing ring groove 8111. When the pushing ring 814 moves away from the second connecting portion 812, the pushing ring 814 pushes the pushing piece 817, so that the pushing arc bar 815 slides along the radian of the rotating arc groove 8113, and forces one end of the pushing arc bar 815 away from the pushing ring 814 to abut against the inner peripheral wall of the sampling hose 12, and the pushing arc bar 815 has a driving force for the sampling hose 12 to move towards the second connecting portion 812.

[0056] The implementation principle of Embodiment 3 of this application is as follows: when the second docking portion 832 screws the docking ring 82, the pushing ring 814 pushes the first sealing ring 813 to move along the guiding surface 8112 towards the side away from the second connecting portion 812, and makes the first sealing ring 813 abut tightly against the inner peripheral wall of the sampling hose 12, resulting in that the sampling hose 12 is subjected to an extrusion force for retraction from the first sealing ring 813; while the pushing ring 814 pushes the first sealing ring 813, the pushing ring 814 can also push the pushing arc bar 815, forcing the pushing arc bar 815 to rotate around its own central axis, so that one end of the pushing arc bar 815 can abut against the sampling hose 12 and form a driving force for driving the sampling hose 12 to move towards the second connecting portion 812. This driving force cancels out the extrusion force received by the sampling hose 12, thereby reducing the possibility of the sampling hose 12 retracting when the first sealing ring 813 abuts tightly against the sampling hose 12, and further improving the connection stability between the sampling hose 12 and the first connecting portion 811.

[0057] Embodiment 4: This application embodiment discloses an automated aseptic sampling docking biochemical analyzer device.

[0058] Referring to Figure 7 , the difference between the automated aseptic sampling docking biochemical analyzer device disclosed in the embodiment of this application and Embodiment 2 is as follows: In this embodiment, a flow splitting ring 816 is detachably installed at one end of the first connection part 811 away from the second connection part 812. The flow splitting ring 816 is coaxially arranged with the first connection part 811, and the inner diameter of the flow splitting ring 816 is smaller than that of the first connection part 811. A plurality of countersunk bolts 8162 are installed on the flow splitting ring 816. The plurality of countersunk bolts 8162 are arranged at intervals around the central axis of the flow splitting ring 816. The countersunk bolts 8162 sequentially pass through the flow splitting ring 816 and the first connection part 811 and are threadedly connected to the first connection part 811.

[0059] The end face of the first connection part 811 away from the second connection part 812 has a first flow splitting surface, and the side wall of the flow splitting ring 816 close to the first connection part 811 has a second flow splitting surface. An isolation ball 8163 is sleeved on the outer wall of the countersunk bolt 8162. The surface of the isolation ball 8163 abuts against the first flow splitting surface and the second flow splitting surface respectively. The isolation ball 8163 spaces the first flow splitting surface of the first connection part 811 and the second flow splitting surface of the flow splitting ring 816 to form a flow splitting channel 8161 for the feed liquid to flow through. The flow splitting channel 8161 is arranged in a ring shape around the central axis of the flow splitting ring 816, and the diameter of the flow splitting channel 8161 gradually increases from the side close to the second connection part 812 to the side away from the second connection part 812.

[0060] The implementation principle of Embodiment 4 of this application is as follows: A flow splitting ring 816 is added at one end of the first connection part 811 away from the second connection part 812 to form a flow splitting channel 8161 for the feed liquid to flow through. After the feed liquid enters the flow splitting channel 8161, it is discharged outward from the outlet end of the flow splitting channel 8161, thereby pushing the feed liquid at the end face position of the first connection part 811 away from the second connection part 812, making it difficult for the feed liquid in the sampling hose 12 to enter the gap between the outer peripheral wall of the first connection part 811 and the inner peripheral wall of the sampling hose 12, and thus improving the sealing performance of the overall structure.

[0061] Embodiment 5: This application embodiment also discloses an automated aseptic sampling method.

[0062] An automated aseptic sampling method, keeping the flow regulating valve 114 in an always-open state, the method mainly includes: Automatic CIP step: Control the third valve 211 and the fourth valve 221 to open, shut off the first valve 111, the second valve 112 and the fifth valve 321, and introduce cleaning liquid from the external cleaning liquid output device 6 to clean the pipeline. After a set duration, control the third valve 211 and the fourth valve 221 to shut off.

[0063] Automatic SIP steps: Control the opening of the third valve 211 and the fifth valve 321, shut off the first valve 111, the second valve 112 and the fourth valve 221, introduce steam from the external steam generator 7 to sterilize the pipeline, obtain the data of the temperature sensor 322, and control the shut-off of the third valve 211 and the fifth valve 321 when it reaches the set value and lasts for the set time.

[0064] Automatic sampling steps: Control the opening of the first valve 111 and the fifth valve 321, shut off the third valve 211 and the fourth valve 221, the liquid material enters the sample temporary storage tube 11 through the first valve 111 and the flow regulating valve 114. When it rises to the set height, shut off the first valve 111, then control the opening of the second valve 112, and use the peristaltic pump in the external biochemical analyzer 5 to automatically extract the liquid material sample in the sample temporary storage tube 11 to complete the sampling.

[0065] Specifically, in the embodiment of the present application, there is a 300 mm vertical pipeline space above the flow regulating valve 114 in the sample temporary storage tube 11. By adjusting the flow of the flow regulating valve 114 and setting the opening time of the first valve 111, the liquid material is allowed to rise to a height of 200 - 300 mm, and a pipeline sight glass is installed at the top position to prevent waste caused by excessive sampling.

[0066] It should be noted that the method disclosed in the embodiment of the present application includes detailed descriptions of each implementation step. The description order of each step should not be understood as a limitation of the specific implementation order. In actual applications, according to needs, the above steps can be executed separately or combined in a selected order.

[0067] The above is the preferred embodiment of the present application. It does not limit the protection scope of the present application accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of the present application should be covered within the protection scope of the present application.

Claims

1. An automated sterile sampling and docking biochemical analyzer device, characterized in that: It includes a controller, a sampling pipeline (1), a CIP pipeline (2), and an SIP pipeline (3). The sampling pipeline (1) includes a sample temporary storage pipe (11) and a sampling hose (12) connected in sequence. The sample temporary storage pipe (11) is connected to a bioreactor (4), and a first valve (111) is arranged between them. One end of the sampling hose (12) is connected to the bottom end of the sample temporary storage pipe (11) through a second valve (112), and the other end is connected to an external biochemical analyzer (5). The CIP pipeline (2) includes a CIP inlet pipe (21) and a CIP return pipe (22). One end of the CIP inlet pipe (21) is connected to an external cleaning liquid output device (6), and the other end is connected to the output end of the first valve (111) through a third valve (211). One end of the CIP return pipe (22) is connected to the top end of the sample temporary storage pipe (11), and the other end is connected to an external CIP return liquid collection device (61) through a fourth valve (221). The SIP pipeline (3) includes an SIP inlet pipe (31) and an SIP return pipe (32). One end of the SIP inlet pipe (31) is connected to an external steam generator (7), and the other end is connected to the CIP inlet pipe (21). One end of the SIP return pipe (32) is connected to the top end of the sample temporary storage pipe (11), and the other end is connected to an external condensate recovery device (71) through a fifth valve (321). Each valve is connected to the controller for control.

2. The automated aseptic sampling and docking biochemical analyzer device according to claim 1, wherein: The sample temporary storage pipe (11) is arranged vertically. The bottom end of the sample temporary storage pipe (11) is connected with a sampling pipe (113). The end of the sampling pipe (113) far from the sample temporary storage pipe (11) is connected to the bioreactor (4). The sampling pipe (113) is arranged obliquely. The first valve (111) is arranged on the sampling pipe (113). The connection point of the sampling pipe (113) and the bioreactor (4) is not higher than the lowest liquid level of the liquid in the bioreactor (4). The bottom end of the sample temporary storage pipe (11) is connected with a flow regulating valve (114).

3. An automated aseptic sampling and docking biochemical analyzer device according to claim 1, characterized in that: The SIP return pipe (32) is sequentially provided with a temperature sensor (322) for detecting the temperature in the pipeline and a steam trap (323) for preventing steam from discharging. The temperature sensor (322) and the steam trap (323) are both located between the fifth valve (321) and the external condensate recovery device (71). The controller is in signal connection with the temperature sensor (322).

4. An automated aseptic sampling and docking biochemical analyzer device according to claim 2, characterized in that: The first valve (111), the second valve (112), the third valve (211), the fourth valve (221), and the fifth valve (321) all adopt pneumatic diaphragm valves. The flow regulating valve (114) adopts a manual diaphragm valve.

5. An automated aseptic sampling and docking biochemical analyzer device according to claim 1, characterized in that: The bottom peripheral wall of the sample temporary storage tube (11) is connected with an installation branch pipe (13). One end of the sampling hose (12) far away from the biochemical analyzer (5) is connected to the installation branch pipe (13). The sampling hose (12) is connected to the sample temporary storage tube (11) through the installation branch pipe (13). A connection assembly (8) is arranged between the installation branch pipe (13) and the sampling hose (12). The sampling hose (12) is detachably installed on the installation branch pipe (13) through the connection assembly (8).

6. The automated aseptic sampling and docking biochemical analyzer device according to claim 5, wherein: The connection assembly (8) includes a connection joint (81), a docking ring (82) and a docking sleeve (83). The connection joint (81) includes a first connection part (811) and a second connection part (812) which are integrally formed. One end of the sampling hose (12) is sleeved on the outer peripheral wall of the first connection part (811). The outer diameter of the sampling hose (12) is smaller than the outer diameter of the second connection part (812). One end of the installation branch pipe (13) is inserted into the second connection part (812). The docking ring (82) is arranged on the outer peripheral wall of the installation branch pipe (13). The outer diameter of the docking ring (82) is adapted to the outer diameter of the second connection part (812). The docking sleeve (83) includes a first docking part (831) and a second docking part (832) which are integrally formed. The first docking part (831) is sleeved on the outer peripheral wall of the sampling hose (12). The second docking part (832) is simultaneously sleeved on the outer peripheral wall of the second connection part (812) and the outer peripheral wall of the docking ring (82) and is threadedly connected with the docking ring (82).

7. An automated aseptic sampling and docking biochemical analyzer device according to claim 6, characterized in that: A first sealing ring groove (8111) is formed on the outer peripheral wall of the first connection part (811). A first sealing ring (813) is arranged in the first sealing ring groove (8111). The first sealing ring groove (8111) has a guiding surface (8112). The guiding surface (8112) has an outer diameter gradually increasing from the side close to the second connection part (812) to the side far away from the second connection part (812). A pushing ring (814) is slidably installed in the first sealing ring groove (8111). The pushing ring (814) is located on the side of the first sealing ring (813) close to the second connection part (812). The second connection part (812) is provided with a driving assembly (9). When the second docking part (832) tightens the docking ring (82), the driving assembly (9) drives the pushing ring (814) to displace away from the second connection part (812), so that the first sealing ring (813) abuts against the inner peripheral wall of the sampling hose (12).

8. An automated aseptic sampling and docking biochemical analyzer device according to claim 7, characterized in that: A rotation arc groove (8113) is formed in the first connection part (811). One end of the rotation arc groove (8113) penetrates through the guiding surface (8112), and the other end penetrates through the outer peripheral wall of the first connection part (811). A pushing arc bar (815) is slidably arranged in the rotation arc groove (8113). When the pushing ring (814) moves away from the second connection part (812), the pushing ring (814) pushes the pushing arc bar (815), so that one end of the pushing arc bar (815) away from the pushing ring (814) abuts against the inner peripheral wall of the sampling hose (12), and has a driving force for the sampling hose (12) to move towards the second connection part (812).

9. The automated aseptic sampling and docking biochemical analyzer device according to claim 6, wherein: A flow dividing ring (816) is arranged at one end of the first connection part (811) away from the second connection part (812). The inner diameter of the flow dividing ring (816) is smaller than the inner diameter of the first connection part (811). A flow dividing channel (8161) is formed between the flow dividing ring (816) and the first connection part (811). The flow dividing channel (8161) is arranged in a ring shape around the central axis of the flow dividing ring (816), and the diameter of the flow dividing channel (8161) gradually increases from the side close to the second connection part (812) to the side away from the second connection part (812).

10. An automated aseptic sampling method, based on an automated aseptic sampling docking biochemical analyzer device according to any one of claims 1-9, includes: Keep the flow regulating valve (114) in an open state all the time; An automatic CIP step: control the third valve (211) and the fourth valve (221) to open, turn off the first valve (111), the second valve (112) and the fifth valve (321), and introduce cleaning liquid from an external cleaning liquid output device (6) to clean the pipeline. After a set duration, control the third valve (211) and the fourth valve (221) to turn off; An automatic SIP step: control the third valve (211) and the fifth valve (321) to open, turn off the first valve (111), the second valve (112) and the fourth valve (221), introduce steam from an external steam generator (7) to sterilize the pipeline, obtain the data of the temperature sensor (322), and control the third valve (211) and the fifth valve (321) to turn off after it reaches the set value and lasts for a set duration; An automatic sampling step: control the first valve (111) and the fifth valve (321) to open, turn off the third valve (211) and the fourth valve (221), the liquid material enters the sample temporary storage tube (11) through the first valve (111) and the flow regulating valve (114). After it rises to the set height, turn off the first valve (111), and then control the second valve (112) to open, and automatically extract the liquid material sample in the sample temporary storage tube (11) with a peristaltic pump in an external biochemical analyzer (5) to complete the sampling.

Images