A device for collecting secreted proteins produced by stem cell differentiation based on a sedimentation method

Abstract

The application relates to the technical field of protein collecting devices, and discloses a protein collecting device based on a precipitation method and used for collecting secreted proteins generated by stem cell differentiation, which comprises a scraper, the surface of the scraper is provided with a guide groove, and the bottom of the scraper is fixedly connected with a flow guide groove plate; the scraper mechanism is arranged; when the scraper rotates, the inner wall of the collecting cylinder is scraped; when the scraper rotates, the inertia of rotation makes the residual proteins on the inner wall of the collecting cylinder scraped to the inner wall of the guide groove; when the proteins flow to the guide groove, the proteins flow downwards along the guide groove; the vibration caused by the impact of the contact telescopic rod on the surface of the collecting cylinder is used to make the proteins on the inner wall of the guide groove flow downwards quickly, so that the proteins on the inner wall of the collecting cylinder are collected quickly; the residual proteins are effectively collected and damaged is reduced; the cleaning brush is used to clean the inner wall of the collecting cylinder, the cleaning time is saved, and the efficiency of the next use is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of protein collection equipment, in particular to a protein collection device based on the precipitation method for stem cell differentiation and protein secretion. BACKGROUND

[0002] Proteins are one of the most basic molecular components in living organisms, playing an important role in regulating cell function and signal transmission. Proteomics plays an important role in the development of biological drugs, and can be used to identify and quantify drug candidates, monitor protein expression and quality consistency, etc. The application of proteomics can improve the efficiency and success rate of drug development.

[0003] In the prior art, when the protein is precipitated, it needs to be collected. However, after the protein is taken out of the collection device, some protein will still be left in the collection device and adhere to the inner wall of the collection device. When the time is long, the residual protein will be damaged. At this time, the residual protein needs to be collected, and manual collection is easy to damage the protein. SUMMARY

[0004] The purpose of the present application is to provide a protein collection device based on the precipitation method for stem cell differentiation and protein secretion to solve the problems raised in the background art.

[0005] To solve the above technical problems, the present application is realized by the following technical scheme:

[0006] The present application is a protein collection device based on the precipitation method for stem cell differentiation and protein secretion, comprising a collection cylinder, a support leg fixedly connected to the surface of the collection cylinder, a bottom plate fixedly connected to the bottom of the support leg, a support frame fixedly connected to the surface of the bottom plate, and a motor fixedly connected to the top of the support frame.

[0007] The vibration mechanism comprises a moving plate, a driven push rod rotatably connected to the surface of the moving plate, and a contact telescopic rod rotatably connected to the end of the driven push rod away from the moving plate.

[0008] The scraping mechanism comprises a scraper, a guide groove is formed in the surface of the scraper, and a flow guide groove plate is fixedly connected to the bottom of the scraper.

[0009] The collection mechanism comprises a threaded plate, a scraper ring fixedly connected to both ends of the threaded plate, and a push plate rotatably connected to the bottom of the threaded plate.

[0010] Further, the number of support frames is four, the four support frames are symmetrically arranged with the bottom plate as the center, a receiving hole is formed in the bottom of the inner wall of the collection cylinder, and the number of support legs is three.

[0011] Further, the vibration mechanism comprises a transmission shaft, the surface of the transmission shaft is fixedly connected with a threaded ring, the surface of the threaded ring is fixedly connected with a rotating ring, the bottom of the rotating ring is fixedly connected with an extrusion bent rod, the inner wall of the support frame is fixedly connected with a sliding groove plate, the top of the moving plate is fixedly connected with a stress inclined plate, the bottom of the moving plate is fixedly connected with a return spring.

[0012] Further, the end of the transmission shaft is fixedly connected with the output end of the motor, the two ends of the moving plate are slidingly connected with the inner wall of the sliding groove plate, the end of the extrusion bent rod away from the rotating ring is in contact with the surface of the stress inclined plate, the end of the contact telescopic rod away from the driven push rod is fixedly connected with the inner wall of the support frame.

[0013] Further, the scraping mechanism comprises a rotating shaft, the surface of the rotating shaft is fixedly connected with a driven ring, the surface of the driven ring is fixedly connected with a rotating rod, the end of the rotating rod away from the driven ring is fixedly connected with a cleaning plate, the surface of the cleaning plate is fixedly connected with a cleaning brush.

[0014] Further, the end of the rotating shaft is fixedly connected with the end of the transmission shaft, the surface of the scraping plate is fixedly connected with the surface of the rotating rod, the side of the scraping plate away from the rotating rod is in contact with the inner wall of the collecting cylinder, the end of the cleaning brush away from the cleaning plate is in contact with the inner wall of the collecting cylinder.

[0015] Further, the collecting mechanism comprises a threaded rod, the end of the pushing plate away from the threaded plate is rotationally connected with a collecting contraction ring, the bottom of the threaded plate is fixedly connected with a limiting spring, the bottom of the collecting cylinder is provided with a receiving box, the inner wall of the collecting cylinder is fixedly connected with a drainage inclined plate.

[0016] Further, the end of the rotating shaft away from the transmission shaft is fixedly connected with the end of the threaded rod, the bottom of the collecting contraction ring is in contact with the bottom of the inner wall of the collecting cylinder, the outer wall of the scraping ring is in contact with the inner wall of the collecting cylinder, the end of the threaded rod away from the rotating shaft is rotationally connected with the inner wall of the collecting cylinder, the top of the receiving box is in contact with the bottom of the collecting cylinder, the bottom of the receiving box is in contact with the surface of the bottom plate.

[0017] The present application has the following advantages:

[0018] The present application is characterized in that the vibration mechanism is provided, first starting the motor to drive the transmission shaft to rotate, when the transmission shaft rotates, the threaded ring is driven to rotate, when the threaded ring rotates, the rotating ring is driven to rotate, when the rotating ring rotates, the stress inclined plate is extruded, when the stress inclined plate is extruded, the moving plate is pushed to slide downward in the inner wall of the sliding groove plate, when the moving plate slides, the driven push rod is pushed to move away from each other, when the driven push rod moves, the contact telescopic rod is pushed to extend towards each other and contact the surface of the collection cylinder, when the moving plate slides, the reset spring is extruded, when the reset spring is extruded, it shrinks downward, when the extrusion bending rod ends the extrusion of the stress inclined plate, the moving plate has no extrusion force and is reset to the original position by the elastic force of the reset spring, at the same time, the driven push rod pulls the contact telescopic rod back to the original position, since the transmission shaft drives the threaded ring to keep rotating, the rotating ring drives the extrusion bending rod to keep rotating and extrude the stress inclined plate, since the extrusion bending rod is provided with a plurality of extrusion bending rods and each extrusion bending rod is spaced a certain distance, the stress inclined plate is extruded, so that the stress inclined plate pushes the moving plate to reciprocatingly slide, when the moving plate reciprocatingly slides, the driven push rod reciprocatingly moves, at the same time, the driven push rod reciprocatingly extends the contact telescopic rod and slightly impacts the surface of the collection cylinder, so that the inner wall of the collection cylinder vibrates, and the protein adhered to the inner wall of the collection cylinder flows downward when it feels the vibration, thereby improving the efficiency of collecting the protein remaining in the inner wall of the collection cylinder.

[0019] The present application is characterized in that the vibration mechanism is provided, first starting the motor to drive the transmission shaft to rotate, when the transmission shaft rotates, the threaded ring is driven to rotate, when the threaded ring rotates, the rotating ring is driven to rotate, when the rotating ring rotates, the stress inclined plate is extruded, when the stress inclined plate is extruded, the moving plate is pushed to slide downward in the inner wall of the sliding groove plate, when the moving plate slides, the driven push rod is pushed to move away from each other, when the driven push rod moves, the contact telescopic rod is pushed to extend towards each other and contact the surface of the collection cylinder, when the moving plate slides, the reset spring is extruded, when the reset spring is extruded, it shrinks downward, when the extrusion bending rod ends the extrusion of the stress inclined plate, the moving plate has no extrusion force and is reset to the original position by the elastic force of the reset spring, at the same time, the driven push rod pulls the contact telescopic rod back to the original position, since the transmission shaft drives the threaded ring to keep rotating, the rotating ring drives the extrusion bending rod to keep rotating and extrude the stress inclined plate, since the extrusion bending rod is provided with a plurality of extrusion bending rods and each extrusion bending rod is spaced a certain distance, the stress inclined plate is extruded, so that the stress inclined plate pushes the moving plate to reciprocatingly slide, when the moving plate reciprocatingly slides, the driven push rod reciprocatingly moves, at the same time, the driven push rod reciprocatingly extends the contact telescopic rod and slightly impacts the surface of the collection cylinder, so that the inner wall of the collection cylinder vibrates, and the protein adhered to the inner wall of the collection cylinder flows downward when it feels the vibration, thereby improving the efficiency of collecting the protein remaining in the inner wall of the collection cylinder.

[0020] The application sets the collecting mechanism, when the rotating shaft rotates, the threaded rod rotates, when the threaded rod rotates, the threaded plate moves downward, when the threaded plate moves, the scraper ring moves downward, when the scraper ring moves, the lower surface of the inner wall of the collecting cylinder is scraped, the protein on the inner wall is scraped downward, and the protein flows downward under the guidance of the drainage inclined plate, at this time, the threaded plate moves to the position without thread on the surface of the threaded rod, the threaded plate is kept stationary through the limiting of the limiting spring, at this time, the threaded rod keeps rotating, at the same time, the scraper ring moves to the upper side of the drainage inclined plate and keeps stationary and does not contact with the drainage inclined plate, when the scraper ring moves downward, the push plate moves downward, when the push plate moves, the collecting shrink ring is pushed to shrink in the direction of approaching each other, so that the flowing protein is concentrated and pushed to the inner wall of the storage box, effectively achieving the quick collection of the residual protein and reducing the damage of the protein.

[0021] Of course, implementing any product of the present application does not necessarily need to achieve all the above advantages at the same time. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed for the embodiment description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0023] Figure 1 It is a schematic diagram of the overall structure of the present application;

[0024] Figure 2 It is a schematic diagram of the overall structure of the present application;

[0025] Figure 3 It is a schematic diagram of the overall structure of the present application;

[0026] Figure 4 It is a schematic diagram of the driven push rod structure of the present application;

[0027] Figure 5 It is a schematic diagram of the overall structure of the present application;

[0028] Figure 6 It is a schematic diagram of the overall structure of the present application;

[0029] Figure 7 It is a schematic diagram of the overall structure of the present application;

[0030] Figure 8 It is a schematic diagram of the overall structure of the present application;

[0031] Figure 9 It is a schematic diagram of the overall structure of the present application.

[0032] The components represented by the reference numbers in the drawings are listed as follows:

[0033] In the drawings: 1, collecting cylinder; 2, supporting leg; 3, bottom plate; 4, supporting frame; 5, motor; 10, vibrating mechanism; 11 transmission shaft; 12, threaded ring; 13, rotating ring; 14, extruded bent rod; 15, sliding groove plate; 16, moving plate; 17, force- bearing inclined plate; 18, driven push rod; 19, contact telescopic rod; 20, return spring; 30, scraping mechanism; 31, rotating shaft; 32, driven ring; 33, rotating rod; 34, cleaning plate; 35, cleaning brush; 36, scraper; 37, flow guide groove plate; 50, collecting mechanism; 51, threaded rod; 52, threaded plate; 53, scraper ring; 54, push plate; 55, collecting shrink ring; 56, limiting spring; 57, storage box; 58, drainage inclined plate. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0035] Please refer to Figure 1 - Figure 9 As shown in the drawings, the present application is a kind of based on the collection device of protein secreted by stem cell differentiation produced by precipitation method, including collecting cylinder 1, the surface of collecting cylinder 1 is fixedly connected with supporting leg 2, the bottom of supporting leg 2 is fixedly connected with bottom plate 3, the surface of bottom plate 3 is fixedly connected with supporting frame 4, the top of supporting frame 4 is fixedly connected with motor 5, further comprising;

[0036] Vibrating mechanism 10, vibrating mechanism 10 includes moving plate 16, when force- bearing inclined plate 17 is extruded, moving plate 16 will be pushed to slide down in the inner wall of sliding groove plate 15, the surface of moving plate 16 is rotatably connected with driven push rod 18, when moving plate 16 slides, driven push rod 18 will be pushed to move away from each other, the end of driven push rod 18 away from moving plate 16 is rotatably connected with contact telescopic rod 19, when driven push rod 18 moves, contact telescopic rod 19 will be pushed to extend towards each other and contact with the surface of collecting cylinder 1;

[0037] The scraping mechanism 30 comprises a scraper 36. The bottom plate 3 rotates to drive the scraper 36 to rotate. The surface of the scraper 36 is provided with a guide groove. When the scraper 36 rotates, the inner wall of the collecting cylinder 1 is scraped. When the scraper 36 rotates, the inertia of rotation causes the protein remaining on the inner wall of the collecting cylinder 1 to be scraped to the inner wall of the guide groove. When the protein flows to the guide groove, it flows downward along the guide groove. The guide groove is in contact with the telescopic rod 19. The vibration caused by the impact of the telescopic rod 19 on the surface of the collecting cylinder 1 causes the protein on the inner wall of the guide groove to flow downward quickly. The bottom of the scraper 36 is fixedly connected with a flow guide groove plate 37.

[0038] The collecting mechanism 50 comprises a threaded plate 52. When the threaded rod 51 rotates, the threaded plate 52 moves downward. The two ends of the threaded plate 52 are fixedly connected with a scraper ring 53. When the threaded plate 52 moves, the scraper ring 53 moves downward. When the scraper ring 53 moves, the lower surface of the inner wall of the collecting cylinder 1 is scraped downward. The bottom of the threaded plate 52 is rotatably connected with a push plate 54. When the scraper ring 53 moves downward, the push plate 54 moves downward.

[0039] The number of support frames 4 is four. The four support frames 4 are symmetrically arranged around the bottom plate 3. The bottom of the inner wall of the collecting cylinder 1 is provided with a receiving hole. The number of support feet 2 is three.

[0040] The vibration mechanism 10 comprises a transmission shaft 11. First, the motor 5 drives the transmission shaft 11 to rotate. The surface of the transmission shaft 11 is fixedly connected with a threaded ring 12. When the transmission shaft 11 rotates, the threaded ring 12 rotates. The surface of the threaded ring 12 is fixedly connected with a rotating ring 13. When the threaded ring 12 rotates, the rotating ring 13 rotates. The bottom of the rotating ring 13 is fixedly connected with a squeezed bent rod 14. At the same time, the rotating ring 13 drives the squeezed bent rod 14 to rotate, which squeezes the stress inclined plate 17. The inner wall of the support frame 4 is fixedly connected with a sliding groove plate 15. The top of the moving plate 16 is fixedly connected with the stress inclined plate 17. When the rotating ring 13 rotates, the stress inclined plate 17 is squeezed. The bottom of the moving plate 16 is fixedly connected with a return spring 20. When the moving plate 16 slides, the return spring 20 is squeezed. When the return spring 20 is squeezed, it shrinks downward. When the moving plate 16 reciprocally slides, the driven push rod 18 reciprocally moves. At the same time, the driven push rod 18 pushes the contact telescopic rod 19 to reciprocally extend, which slightly impacts the surface of the collecting cylinder 1. Thus, the inner wall of the collecting cylinder 1 vibrates, which causes the protein adhering to the inner wall of the collecting cylinder 1 to flow downward when it feels the vibration, thereby improving the efficiency of collecting the protein remaining on the inner wall of the collecting cylinder 1.

[0041] The end of the transmission shaft 11 is fixedly connected with the output end of the motor 5. Since the transmission shaft 11 drives the threaded ring 12 to rotate, the two ends of the moving plate 16 are slidably connected with the inner wall of the sliding groove plate 15. At this time, the moving plate 16 is not pressed and is reset to the original position by the elastic force of the reset spring 20. The end of the extrusion bending rod 14 away from the rotating ring 13 is in contact with the surface of the stress inclined plate 17. Since the extrusion bending rod 14 is provided with a plurality of extrusion bending rods 14 and each extrusion bending rod 14 is spaced apart by a certain distance, the stress inclined plate 17 is extruded at this time, so that the stress inclined plate 17 drives the moving plate 16 to reciprocatingly slide. When the extrusion bending rod 14 ends the extrusion of the stress inclined plate 17, the end of the contact telescopic rod 19 away from the driven push rod 18 is fixedly connected with the inner wall of the support frame 4, and at the same time, the driven push rod 18 drives the contact telescopic rod 19 to return to the original position.

[0042] The scraping mechanism 30 comprises a rotating shaft 31. When the transmission shaft 11 rotates, the rotating shaft 31 is driven to rotate. The surface of the rotating shaft 31 is fixedly connected with a driven ring 32. When the rotating shaft 31 rotates, the driven ring 32 is driven to rotate. The surface of the driven ring 32 is fixedly connected with a rotating rod 33. The end of the rotating rod 33 away from the driven ring 32 is fixedly connected with a cleaning plate 34. The surface of the cleaning plate 34 is fixedly connected with a cleaning brush 35. When the cleaning plate 34 rotates, the cleaning brush 35 is driven to rotate, so that the inner wall of the collecting cylinder 1 is cleaned, and the labor is saved and the cleaning efficiency is improved.

[0043] The end of the rotating shaft 31 is fixedly connected with the end of the transmission shaft 11. The surface of the scraper 36 is fixedly connected with the surface of the rotating rod 33. When the driven ring 32 rotates, the rotating rod 33 is driven to rotate. One side of the scraper 36 away from the rotating rod 33 is in contact with the inner wall of the collecting cylinder 1. The end of the cleaning brush 35 away from the cleaning plate 34 is in contact with the inner wall of the collecting cylinder 1. When the rotating rod 33 rotates, the cleaning plate 34 is driven to rotate, so that the protein on the inner wall of the collecting cylinder 1 is quickly collected. Not only the residual protein is effectively collected and the damage is reduced, but also the cleaning time of the inner wall of the collecting cylinder 1 is saved, the efficiency of the next use is improved.

[0044] The collecting mechanism 50 comprises a threaded rod 51. At this time, the threaded plate 52 moves to a position without threads on the surface of the threaded rod 51. When the rotating shaft 31 rotates, the threaded rod 51 is driven to rotate. The end of the pushing plate 54 away from the threaded plate 52 is rotatably connected with a collecting contraction ring 55. The bottom of the threaded plate 52 is fixedly connected with a limiting spring 56. The bottom of the collecting cylinder 1 is provided with a storage box 57. The inner wall of the collecting cylinder 1 is fixedly connected with a drainage inclined plate 58, and the protein is guided to flow downward through the drainage of the drainage inclined plate 58.

[0045] The end of the rotating shaft 31 away from the transmission shaft 11 is fixedly connected with the end of the threaded rod 51, and the threaded plate 52 is kept stationary through the limiting of the limiting spring 56, at this time the threaded rod 51 keeps rotating, the bottom of the collection contraction ring 55 is in contact with the bottom of the inner wall of the collection cylinder 1, the outer wall of the scraping ring 53 is in contact with the inner wall of the collection cylinder 1, at the same time the scraping ring 53 moves to the top of the drainage inclined plate 58 and keeps stationary and is not in contact with the drainage inclined plate 58, the end of the threaded rod 51 away from the rotating shaft 31 is rotatably connected with the inner wall of the collection cylinder 1, the top of the storage box 57 is in contact with the bottom of the collection cylinder 1, and the bottom of the storage box 57 is in contact with the surface of the bottom plate 3, when the pushing plate 54 moves, the collection contraction ring 55 is pushed to move in the direction of approaching each other and is contracted, so that the flowing protein is concentrated and pushed to the inner wall of the storage box 57, effectively achieving the rapid collection of the residual protein and reducing the damage of the protein.

[0046] In use, first start the motor 5 drive shaft 11 rotation, when the transmission shaft 11 rotation will drive screw ring 12 rotation, when the screw ring 12 rotation will drive the rotation of the ring 13, when the ring 13 rotation will extrude the force on the inclined plate 17, when the force on the inclined plate 17 is extruded will push the moving plate 16 in the inner wall of the sliding chute plate 15 down, when the moving plate 16 slide will push the driven push rod 18 to the direction of mutual moving, when the driven push rod 18 moves will push the contact telescopic rod 19 to the direction of mutual extension, and with the surface of the collection cylinder 1 contact, when the moving plate 16 slide at the same time will extrude the reset spring 20, when the reset spring 20 is extruded will shrink down, when the extrusion of the bending rod 14 ends the extrusion of the force on the inclined plate 17, at this time the moving plate 16 has no extrusion force, will be reset to the original position through the elastic force of the reset spring 20, at the same time the driven push rod 18 will pull the contact telescopic rod 19 back to the original position, because the transmission shaft 11 will drive the screw ring 12 keep rotating, at the same time the rotation of the ring 13 will drive the extrusion of the bending rod 14 keep rotating, and extrude the force on the inclined plate 17, because the extrusion of the bending rod 14 is set several, and each extrusion of the bending rod 14 is spaced a certain distance, at this time will extrude the force on the inclined plate 17, so that the force on the inclined plate 17 push the moving plate 16 reciprocating slide, when the moving plate 16 reciprocating slide will push the driven push rod 18 reciprocating movement, at the same time the driven push rod 18 will push the contact telescopic rod 19 reciprocating extension, and the surface of the collection cylinder 1 impact, so that the inner wall of the collection cylinder 1 produce vibration, when the transmission shaft 11 rotation will drive the rotation of the shaft 31, when the shaft 31 rotation will drive the driven ring 32 rotation, when the driven ring 32 rotation will drive the rotation of the rod 33, when the rotation of the rod 33 will drive the rotation of the cleaning plate 34, when the cleaning plate 34 rotation will drive the rotation of the cleaning brush 35, so as to clean the inner wall of the collection cylinder 1, and save the labor and improve the cleaning efficiency, when the bottom plate 3 rotation will drive the scraper 36 rotation, when the scraper 36 rotation will scrape the inner wall of the collection cylinder 1, when the scraper 36 rotation at the same time will make the residual protein in the inner wall of the collection cylinder 1 through the inertia of rotation, when the protein flows to the guide groove will flow down along the guide groove, and cooperate with the contact telescopic rod 19 impact on the surface of the collection cylinder 1 vibration, make the protein in the inner wall of the guide groove flow down quickly, so as to collect the protein on the inner wall of the collection cylinder 1 quickly, when the rotation of the shaft 31 will drive the screw rod 51 rotation, when the screw rod 51 rotation will make the screw plate 52 move down, when the screw plate 52 moves will pull the scraper ring 53 down, when the scraper ring 53 moves will scrape the lower surface of the inner wall of the collection cylinder 1 down, and through the guide of the drainage inclined plate 58 make the protein flow down, at this time the screw plate 52 moves to the position without thread on the surface of the screw rod 51, through the limiting of the limiting spring 56 make the screw plate 52 keep static, at this time the screw rod 51 keep rotating,Meanwhile, the scraping ring 53 moves to above the drainage inclined plate 58 and remains stationary without contacting the drainage inclined plate 58. When the scraping ring 53 moves downward, the pushing plate 54 is pushed to move downward. When the pushing plate 54 moves, the collecting shrinking ring 55 is pushed to shrink toward each other.

[0047] The preferred embodiments of the application disclosed above are only used to help explain the application. The preferred embodiments do not describe all the details and limit the application to the specific embodiments. Obviously, many modifications and variations can be made according to the content of the present application. The present application selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present application, so that those skilled in the art can well understand and utilize the present application. The present application is limited only by the claims and their full scope and equivalents.

Claims

1. A protein collection device for stem cell differentiation and secretion based on precipitation method, comprising a collection cylinder (1), a support foot (2) fixedly connected to the surface of the collection cylinder (1), a base plate (3) fixedly connected to the bottom of the support foot (2), a support frame (4) fixedly connected to the surface of the base plate (3), and a motor (5) fixedly connected to the top of the support frame (4), characterized in that, Also includes; The vibration mechanism (10) includes a moving plate (16) and a transmission shaft (11), the surface of the moving plate (16) is rotatably connected with a driven push rod (18), one end of the driven push rod (18) away from the moving plate (16) is rotatably connected with a contact telescopic rod (19), the surface of the transmission shaft (11) is fixedly connected with a threaded ring (12), the surface of the threaded ring (12) is fixedly connected with a rotating ring (13), the bottom of the rotating ring (13) is fixedly connected with an extrusion bent rod (14), the inner wall of the support frame (4) is fixedly connected with a sliding groove plate (15), the top of the moving plate (16) is fixedly connected with a stress inclined plate (17), the bottom of the moving plate (16) is fixedly connected with a reset spring (20); The scraping mechanism (30) includes a scraper (36) and a rotating shaft (31), the surface of the scraper (36) is provided with a guide groove, the bottom of the scraper (36) is fixedly connected with a flow guide groove plate (37), the surface of the rotating shaft (31) is fixedly connected with a driven ring (32), the surface of the driven ring (32) is fixedly connected with a rotating rod (33), one end of the rotating rod (33) away from the driven ring (32) is fixedly connected with a cleaning plate (34), the surface of the cleaning plate (34) is fixedly connected with a cleaning brush (35); The collecting mechanism (50) includes a threaded plate (52) and a threaded rod (51), both ends of the threaded plate (52) are fixedly connected with a scraping ring (53), the bottom of the threaded plate (52) is rotatably connected with a push plate (54), one end of the push plate (54) away from the threaded plate (52) is rotatably connected with a collecting contraction ring (55), the bottom of the threaded plate (52) is fixedly connected with a limiting spring (56), the bottom of the collecting cylinder (1) is provided with a storage box (57), the inner wall of the collecting cylinder (1) is fixedly connected with a drainage inclined plate (58).

2. A device for collecting secreted proteins produced by stem cell differentiation based on the sedimentation method according to claim 1, characterized in that: The number of support frames (4) is four, the four support frames (4) are symmetrically arranged with the bottom plate (3) as the center, the bottom of the inner wall of the collecting cylinder (1) is provided with a storage hole, and the number of supporting legs (2) is three.

3. A device for collecting secreted proteins produced by stem cell differentiation based on the sedimentation method according to claim 2, characterized in that: The end of the transmission shaft (11) is fixedly connected with the output end of the motor (5), both ends of the moving plate (16) are slidably connected with the inner wall of the sliding groove plate (15), one end of the extrusion bent rod (14) away from the rotating ring (13) is in contact with the surface of the stress inclined plate (17), and one end of the contact telescopic rod (19) away from the driven push rod (18) is fixedly connected with the inner wall of the support frame (4).

4. A device for collecting secreted proteins produced by stem cell differentiation based on the sedimentation method according to claim 3, characterized in that: The end of the rotating shaft (31) is fixedly connected with the end of the transmission shaft (11), the surface of the scraper (36) is fixedly connected with the surface of the rotating rod (33), one side of the scraper (36) away from the rotating rod (33) is in contact with the inner wall of the collecting cylinder (1), and one end of the cleaning brush (35) away from the cleaning plate (34) is in contact with the inner wall of the collecting cylinder (1).

5. A device for collecting secreted proteins produced by stem cell differentiation based on the sedimentation method according to claim 4, characterized in that: The rotating shaft (31) is fixedly connected with the end of the threaded rod (51) away from the transmission shaft (11), the bottom of the collecting and contracting ring (55) is in contact with the bottom of the inner wall of the collecting cylinder (1), the outer wall of the scraping ring (53) is in contact with the inner wall of the collecting cylinder (1), the end of the threaded rod (51) away from the rotating shaft (31) is rotatably connected with the inner wall of the collecting cylinder (1), the top of the storage box (57) is in contact with the bottom of the collecting cylinder (1), and the bottom of the storage box (57) is in contact with the surface of the bottom plate (3).

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