Hydrogel based on egg membrane component as well as preparation method and application of hydrogel

Abstract

The invention provides hydrogel based on egg membrane components as well as a preparation method and application of the hydrogel. Based on the advantages of hydrogel, an egg membrane is prepared into a soluble component, and a hydrogel preparation technology is further combined, so that a soluble egg membrane aqueous solution is prepared into the hydrogel with the photo-crosslinking characteristic. The hydrogel has better biocompatibility and biological activity, has a positive effect on tissue regeneration aspects such as wound repair and soft tissue repair, can effectively realize a tissue regeneration function, can also be used as a base material to load other active ingredients, and has a good application prospect. And meanwhile, other biomedical materials can be further prepared through technologies such as electrostatic spinning and electrostatic spraying as a raw material.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedical materials, relates to the preparation and application of hydrogels, and particularly relates to a hydrogel based on egg membrane components, a preparation method thereof, and an application thereof. Background Art

[0002] As a waste resource in the food industry, an eggshell is composed of a mineralized layer on the outer layer of the eggshell and an eggshell membrane close to the egg white. Among them, the eggshell membrane, as a semi-permeable membrane that shows flexibility in a wet state, is regarded as phoenix clothing in traditional Chinese medicine and can treat skin diseases such as burns and pressure sores. This is because the egg membrane contains fibrin, keratin, mucopolysaccharide, and some other bioactive molecules. Among them, mucopolysaccharide has a certain anti-inflammatory function, and the protein component can promote tissue collagen regeneration, indicating that the egg membrane has the potential for application in tissue regeneration. However, the egg membrane has defects such as being brittle and difficult to preserve in a dry state. In a wet state, although the egg membrane has certain skin disease treatment capabilities, the egg membrane is prone to losing water and drying out, has poor skin adhesion, and has a long degradation period, making it difficult to exert all its medicinal effects, thus limiting the wide application of the egg membrane in the treatment of skin diseases.

[0003] In the prior art, certain explorations have been carried out on the biological expansion application of the egg membrane in the skin field. For example, the Chinese invention patent with the patent number CN107149696A discloses an egg membrane coated with a copper-containing bioactive glass nanocoating, which can be used as a skin tissue engineering dressing material and is composed of an egg membrane with a fibrous porous structure and a copper-containing bioactive glass nanocoating deposited on its surface. In the copper-containing bioactive glass nanocoating, copper is uniformly distributed in the Si-Ca-P bioactive glass in the form of Cu 2+ However, this material uses a natural egg membrane as a substrate, which is difficult to prepare a large-area dressing, cannot overcome the defects of poor skin adhesion and long degradation period, and at the same time, the high-cost preparation raw materials also limit its further popularization and application.

[0004] As a common commercial dressing, a hydrogel can provide a wet repair environment for wound healing through its high water content characteristics, and can also perfectly fit with irregular wounds. By regulating the crosslinking degree of the hydrogel, controllable degradation can be achieved, and thus significant regeneration potential can be shown in the wound regeneration environment. If the advantages of the hydrogel and the egg membrane can be combined, it will greatly promote the resource utilization of the egg membrane. Summary of the Invention

[0005] In view of the above problems, based on the advantages of the hydrogel, the present invention prepares the egg membrane into a soluble component, and further combines the hydrogel preparation technology to prepare a hydrogel with photo-crosslinking characteristics from the soluble egg membrane aqueous solution, so as to realize the regeneration application in various tissues.

[0006] After separating the egg membrane, the present invention first treats it with sodium sulfide to obtain a water-soluble egg membrane freeze-dried powder, then conducts acrylation treatment for gelation, and then treats it with methacrylic anhydride to obtain an acrylated egg membrane freeze-dried powder (GE freeze-dried powder). After configuring the GE into an aqueous solution and mixing it with a photoinitiator, it is crosslinked under light treatment at a specific wavelength to obtain a photo-crosslinked egg membrane hydrogel (GEH). This hydrogel GEH has better biocompatibility and bioactivity, plays a positive role in tissue regeneration such as wound repair and soft tissue repair, can effectively achieve the tissue regeneration function, can also be used as a substrate to load other active ingredients, and can also be used as a raw material to further prepare other biomedical materials.

[0007] Based on this, the technical solutions claimed by the present invention are as follows:

[0008] In the first aspect of the present invention, a preparation method of a hydrogel based on egg membrane components is provided, including the following steps:

[0009] (1) Egg membrane acquisition and pulverization treatment: Collect waste eggshells, remove impurities through selection and cleaning; then transfer the eggshells to an acid solution with a certain concentration for treatment for a certain time to dissolve the eggshells, facilitating the separation of the egg membrane from the eggshell to obtain the egg membrane; after drying the egg membrane, grind it into egg membrane powder for standby, which helps to increase the solubility of the egg membrane subsequently.

[0010] Among them, the acid solution is selected from one or more of formic acid, acetic acid, and hydrochloric acid solution. In the specific implementation part of the present invention, acetic acid is used as the acid treatment solution, the volume concentration of the acid solution is 1% - 7%, the treatment time is 6 - 24 h; after drying the egg membrane, grind it in a grinder at 120 HZ for 5 min to obtain egg membrane powder.

[0011] (2) Preparation of egg membrane solution: Disperse the egg membrane powder in an egg membrane dissolving agent with a certain concentration and treat it at a certain temperature for a certain time to obtain an egg membrane aqueous solution.

[0012] In the specific implementation part of the present invention, sodium sulfide is used as the egg membrane dissolving agent, and the mass ratio of sodium sulfide to the egg membrane is 1:1 - 1:5. After mixing, stir and treat it in a water bath at 50 - 65 °C for 24 - 48 h. However, the reagent for dissolving the egg membrane components in the present invention is not limited to sodium sulfide, and other components can also be used, such as protease solution.

[0013] (3) Preparation of water-soluble egg membrane powder: Transfer the egg membrane aqueous solution containing the egg membrane dissolving agent to a dialysis bag for dialysis treatment for a certain time to obtain an egg membrane aqueous solution, and further process it to obtain a water-soluble egg membrane freeze-dried powder.

[0014] Preferably, the residual sodium sulfide component in the solution is removed by dialysis. The molecular weight cut-off of the dialysis bag is 6 - 14 KDa, and the dialysis time is 2 - 5 days. The solution after dialysis is centrifuged or filtered to remove insoluble impurities, where the centrifugation parameters are 8000 - 12000 rmp and the time is 5 - 10 min.

[0015] The eggshell membrane aqueous solution is obtained as eggshell membrane powder through centrifugation or freeze-drying process. When using the freeze-drying process, the eggshell membrane aqueous solution is transferred to a container, cold-treated at -70 to -80 °C for 12 - 24 h, and then transferred to a freeze dryer for 24 - 48 h to obtain water-soluble freeze-dried eggshell membrane powder.

[0016] (4) Acrylation treatment of water-soluble eggshell membrane: The eggshell membrane powder is prepared into an aqueous solution with a concentration of 2% - 8% (w / v), and then methyl methacrylic anhydride with a concentration of 0.2 - 2% (v / v) is added. The reaction is carried out at 30 - 45 °C in the dark for 2 - 6 h to obtain an acrylated eggshell membrane aqueous solution. Then, the acrylated eggshell membrane aqueous solution is transferred to a dialysis bag with a molecular weight of 6 - 14 KDa for 2 - 5 days, and the obtained GE solution after dialysis is freeze-dried to obtain GE freeze-dried powder.

[0017] In the present invention, the treatment of the water solubility of the eggshell membrane is not limited to acrylation. Other methods can also be used to prepare hydrogels, such as using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide / N-hydroxysuccinimide (EDC / NHS) reaction to prepare a viscous hydrogel.

[0018] (5) Preparation of eggshell membrane hydrogel: GE is prepared into an aqueous solution with a certain concentration, a certain proportion of photoinitiator is added, and an efficacy factor is selectively added. Subsequently, the GE solution containing the photoinitiator is transferred to a mold and crosslinked under light treatment with a specific wavelength to obtain a photo-crosslinked eggshell membrane hydrogel GEH.

[0019] Preferably, the photoinitiator is selected from lithium phenyl(2,4,6-trimethylbenzoyl)phosphate LAP or benzophenone. The mass ratio of GE freeze-dried powder to the photoinitiator is 400:1, and the photoinduction condition is 405 nm blue light treatment for 70 - 100 s.

[0020] In the present invention, the prepared hydrogel GEH can be used as a single component. In vitro biocompatibility evaluation shows that GEH can support the growth of L-929 and HUVECs cells, and the cells have good activity ( Figure 3 ); in vivo implantation experiments show that the GEH hydrogel has good biocompatibility and degradation characteristics, and collagen is generated during the degradation process, thus having the potential for in vivo tissue filling applications ( Figure 4); In wound regeneration and repair, compared with the control group gauze, GEH has the ability to significantly promote wound healing on the 14th day ( Figure 5 ).

[0021] Of course, the prepared hydrogel GEH is not limited to a single component. It can be further compounded with other components or bioactive efficacy factors added to prepare a hydrogel with multiple biological functions. The efficacy factors can include anti-inflammatory factors, topical recombinant human epidermal growth factor, recombinant human granulocyte-macrophage stimulating factor, etc. In wound regeneration and repair, compared with the control group gauze, GEH added with the anti-inflammatory peptide AI has a better ability to promote wound healing than the single-component GEH on the 14th day, which is because the addition of the anti-inflammatory peptide AI endows the hydrogel GEH with anti-inflammatory function ( Figure 5 ).

[0022] In the second aspect of the present invention, a hydrogel based on egg membrane components is provided, which is prepared by the method described above. The hydrogel can be a single component or a composite hydrogel added with efficacy factors.

[0023] In the third aspect of the present invention, the application of the above-mentioned hydrogel based on egg membrane components is provided, which can be mainly used in the following aspects:

[0024] (1) Compared with pure egg membrane, the prepared hydrogel GEH has a controllable degradation rate, better biocompatibility and bioactivity. In addition to being used for skin trauma treatment, it can also be used in tissue filling and regeneration applications. Therefore, it can be used to prepare skin wound dressings or tissue regeneration filling materials.

[0025] (2) Compared with pure egg membrane, the prepared hydrogel GEH can load bioactive components in various ways, such as chemical grafting, physical mixing, etc., to achieve efficacy superposition.

[0026] (3) Compared with pure egg membrane, the prepared hydrogel GEH can also be further prepared into other forms of biomedical materials as raw materials, such as being prepared into nanoparticles by combining electrospray and nanofiber membranes by electrospinning technology;

[0027] (4) Based on the characteristics of the hydrogel GEH, it has a positive effect in tissue regeneration such as wound repair and soft tissue repair. Therefore, this hydrogel has significant commercial potential.

[0028] The beneficial guarantees and effects of the present invention are as follows:

[0029] The present invention prepares egg membrane into a soluble aqueous solution through various technical processes and performs acrylation treatment, and then prepares a photocrosslinked hydrogel (GEH) under certain crosslinking conditions, expanding its application form in tissue regeneration. In applications such as wound repair and soft tissue regeneration, it shows good commercial potential. Description of the Drawings

[0030] Figure 1 Shows the morphology of freeze-dried powder of water-soluble egg membrane;

[0031] Figure 2 Shows the appearance of GEH and GEH@AI solutions and hydrogels;

[0032] Figure 3 Shows the in vitro biocompatibility of GEH and GEH@AI;

[0033] Figure 4 Shows the in vivo biocompatibility of GEH;

[0034] Figure 5 Shows the wound repair effects of GEH and GEH@AI hydrogels. Detailed Embodiments

[0035] The following examples and experimental examples further illustrate the present invention and should not be construed as limiting the present invention. The examples do not include a detailed description of traditional methods, such methods being well known to those of ordinary skill in the art and described in many publications.

[0036] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention, and the preferred implementation methods and materials described in the detailed embodiments are for illustrative purposes only.

[0037] Example 1 Preparation of Egg Membrane Aqueous Solution

[0038] 1. Obtaining egg membrane: Obtain discarded eggshells from the cafeteria and cake shop; completely immerse the eggshells in a 5% acetic acid solution at 45 °C for 6 h, and change the acetic acid immersion solution once; perform continuous immersion and cycling treatment 3 times to separate the shell outside the collected eggshells from the egg membrane, and collect the egg membrane;

[0039] 2. Crushing treatment of eggshell membrane: To make the eggshell membrane dissolve better, place the eggshell membrane in a fume hood for 12 h to obtain dry egg membrane; then place it in a grinder and process for 5 min (120 HZ) to obtain egg membrane powder;

[0040] 3. Preparation of egg membrane solution: Add the prepared egg membrane powder and sodium sulfide into an aqueous solution in a mass ratio of 3:1; then process it in a water bath with magnetic stirring for 24 h (65 °C, 240 rpm) to obtain an egg membrane aqueous solution containing sodium sulfide;

[0041] 4. Preparation of water-soluble egg membrane powder: Transfer the egg membrane aqueous solution containing sodium sulfide into a dialysis bag (with a cut-off molecular weight of 8 kDa) to remove the residual sodium sulfide and small molecules that affect the preparation of hydrogel; Dialyze for 5 days, and change the deionized water dialysis solution every 12 h; The completely dialyzed solution is processed by freeze-drying (-80 °C; vacuum degree, -10 - 50 Pa) for 4 days to obtain water-soluble egg membrane powder. The prepared water-soluble egg membrane powder is as Figure 1 shown, presenting as a light yellow powder.

[0042] Example 2 Preparation of Egg Membrane Hydrogel

[0043] 1. Obtaining egg membrane: Obtain waste eggshells from the cafeteria and cake shop; Immerse the eggshells completely in a 5% acetic acid solution at 45 °C for 12 h, and change the acetic acid immersion solution once; Continuously soak and cycle for 3 times to separate the shell on the outer side of the collected eggshells from the egg membrane, and collect the egg membrane;

[0044] 2. Crushing treatment of eggshell membrane: To make the eggshell membrane dissolve better, place the eggshell membrane in a fume hood for 12 h to obtain dry egg membrane; Then place it in a grinder and process for 5 min (120 HZ) to obtain egg membrane powder;

[0045] 3. Preparation of egg membrane solution: Add the prepared egg membrane powder and sodium sulfide into an aqueous solution in a mass ratio of 3:1; then process it in a water bath with magnetic stirring for 24 h (65 °C, 240 rpm) to obtain an egg membrane aqueous solution containing sodium sulfide;

[0046] 4. Preparation of water-soluble egg membrane powder: Transfer the egg membrane aqueous solution containing sodium sulfide into a dialysis bag (with a cut-off molecular weight of 8 kDa) to remove the residual sodium sulfide and small molecules that affect the preparation of hydrogel; Dialyze for 5 days, and change the deionized water dialysis solution every 12 h; The completely dialyzed solution is processed by freeze-drying (-80 °C; vacuum degree, -10 - 50 Pa) for 4 days to obtain water-soluble egg membrane powder;

[0047] 5. Dissolve 1 g of the prepared egg membrane powder in PBS solution at 50 °C. After complete dissolution, prepare a 5% w / v homogeneous solution. Add 0.6 mL of methacrylic anhydride (MA) to the aqueous egg membrane solution at a rate of 0.5 mL / min. Under dark conditions at room temperature, stir continuously for 3 h. After the reaction, dialyze the mixed solution in distilled water at 40 °C for 4 days. Use a dialysis bag (with a molecular weight cut-off of 8 kDa) to remove unreacted methacrylic acid. Finally, the obtained product, methacrylic anhydride-modified egg membrane (GE), is freeze-dried and stored in an ultra-low temperature refrigerator at -80 °C for standby;

[0048] 6. Preparation of egg membrane hydrogel: Dissolve 2 g of GE and the photoinitiator LAP (lithium phenyl-2,4,6-trimethylbenzoylphosphinate) in 10 mL of deionized water, where the content of LAP is 0.05% (w / v); After pouring the prepared hydrogel solution into a mold, treat it with 405 nm blue light for 90 s to obtain the GEH hydrogel. The prepared hydrogel GEH solution is yellow, and the hydrogel still retains yellow after photo-crosslinking to form a gel ( Figure 2 ).

[0049] Example 3 Preparation of Egg Membrane Hydrogel Compound with Bioactive Factors

[0050] 1. Obtaining egg membrane: Obtain waste eggshells from canteens and cake shops; Completely immerse the eggshells in a 5% acetic acid solution at 45 °C for 12 h, and change the acetic acid immersion solution once; Continuously soak and cycle the treatment 3 times to separate the shell on the outer side of the collected eggshells from the egg membrane, and collect the egg membrane;

[0051] 2. Crushing treatment of eggshell membrane: To make the eggshell membrane dissolve better, place the eggshell membrane in a fume hood for 12 h to obtain dry egg membrane; Then place it in a grinder and process for 5 min (120 HZ) to obtain egg membrane powder;

[0052] 3. Preparation of egg membrane solution: Add the prepared egg membrane powder and sodium sulfide to the aqueous solution in a mass ratio of 3:1; Subsequently, treat it in a water bath with magnetic stirring for 24 h (65 °C, 240 rpm) to obtain an aqueous egg membrane solution containing sodium sulfide;

[0053] 4. Preparation of water-soluble egg membrane powder: Transfer the aqueous egg membrane solution containing sodium sulfide to a dialysis bag (with a molecular weight cut-off of 8 kDa) to remove residual sodium sulfide and small molecules that affect hydrogel preparation; Dialyze for 5 days, and change the deionized water dialysis solution every 12 h; The completely dialyzed solution is treated by freeze-drying (-80 °C; vacuum degree, -10 - 50 Pa) for 4 days to obtain water-soluble egg membrane powder;

[0054] 5. Dissolve 1 g of the prepared egg membrane powder in PBS solution at 50 °C. After complete dissolution, prepare a 5% w / v homogeneous solution. Add 0.6 mL of MA to the aqueous egg membrane solution at a rate of 0.5 mL / min. Under dark conditions at room temperature, react with continuous stirring for 3 h. After the reaction, dialyze the mixed solution in distilled water at 40 °C for 4 days. Use a dialysis bag (with a cut-off molecular weight of 8 kDa) to remove unreacted methacrylic acid. The finally obtained product, methacrylic anhydride-modified egg membrane (GE), is subjected to freeze-drying and stored in an ultra-low temperature refrigerator at -80 °C for standby;

[0055] 6. Preparation of egg membrane hydrogel: Dissolve 2 g of GE, photoinitiator LAP (lithium phenyl-2,4,6-trimethylbenzoylphosphinate), and anti-inflammatory peptide (AI) in 10 mL of deionized water, where the content of LAP is 0.05% (w / v). After pouring the prepared anti-inflammatory hydrogel solution into a mold, treat it with 405 nm blue light for 90 s to obtain the GEH@AI hydrogel.

[0056] Effect verification

[0057] 1. In vitro biocompatibility evaluation

[0058] Culture L-929 and HUVECs cells in vitro. Divide the cells into a control group, a GEH group, and a GEH@AI group. Among them, the same concentration of GEH and GEH@AI are added to the GEH group and the GEH@AI group respectively. The results are as Figure 3 shown. Both GEH and GEH@AI can support the growth of L-929 and HUVECs cells, and the cells have good viability.

[0059] 2. In vivo biocompatibility evaluation

[0060] Construct a mouse wound model, and then implant the GEH prepared in Example 2 into the wound. After 14 days, select the wound tissue for HE and Masson staining respectively. The results show that the GEH hydrogel has good biocompatibility and degradation characteristics, and collagen is generated during the degradation process, thus having the potential for in vivo tissue filling application ( Figure 4 ).

[0061] In wound regeneration and repair, compared with the control group gauze, both GEH and GEH@AI have the ability to significantly promote wound healing at day 14. However, the wound healing effect of the GEH@AI group is better because the addition of the anti-inflammatory peptide AI endows the hydrogel GEH with anti-inflammatory function ( Figure 5 ).

[0062] The parts not described in the present invention are the same as or implemented by the prior art. The applicant declares that the present invention illustrates the detailed method of the present invention through the above embodiments, but the present invention is not limited to the above detailed method, that is, it does not mean that the present invention must rely on the above detailed method to be implemented. Those skilled in the art should understand that any improvement to the present invention, the equivalent substitution of each raw material of the product of the present invention, the addition of auxiliary components, the selection of specific methods, etc. all fall within the protection scope and the disclosure scope of the present invention.

Claims

1. A preparation method of a hydrogel based on egg membrane components, characterized in that, It includes the following steps: (1) Egg membrane acquisition and pulverization treatment: Collect waste eggshells, remove impurities through selection and cleaning; then transfer the eggshells to an acid solution with a certain concentration for treatment for a certain time to separate the eggshell from the eggshell membrane to obtain the egg membrane; Dry the egg membrane and then grind it into egg membrane powder for standby; (2) Preparation of egg membrane solution: Disperse the egg membrane powder in an egg membrane solvent with a certain concentration, and treat it at a certain temperature for a certain time to obtain an aqueous egg membrane solution; (3) Preparation of water-soluble egg membrane powder: Transfer the aqueous egg membrane solution containing the egg membrane solvent to a dialysis bag for dialysis treatment for a certain time to obtain an aqueous egg membrane solution, and further process it to obtain freeze-dried water-soluble egg membrane powder; (4) Acrylation treatment of water-soluble egg membrane: Prepare the egg membrane powder into an aqueous solution with a certain concentration, then add a certain amount of methacrylic anhydride, and react for a certain time under light-shielded conditions to obtain an acrylated aqueous egg membrane solution; then transfer the acrylated aqueous egg membrane solution to a dialysis bag for treatment for a certain time, and lyophilize the GE solution obtained after dialysis to obtain GE freeze-dried powder; (5) Preparation of egg membrane hydrogel: Prepare GE into an aqueous solution with a certain concentration, add a certain proportion of photoinitiator, and selectively add functional factors; then transfer the GE solution containing the photoinitiator to a mold and crosslink it under the treatment of light with a specific wavelength to obtain a photo-crosslinked egg membrane hydrogel GEH.

2. The preparation method according to claim 1, characterized in that, In step (1), the acid solution is selected from one or more of formic acid, acetic acid, and hydrochloric acid solution, the volume concentration of the acid solution is 1% - 7%, and the treatment time is 6 - 24 h; After the egg membrane is dried, it is ground in a grinder at 120 HZ for 5 min to obtain egg membrane powder.

3. The preparation method according to claim 1, characterized in that, In step (2), the egg membrane solvent is selected from sodium sulfide or protease; Among them, the mass ratio of sodium sulfide to the egg membrane is 1:1 - 1:5, and after mixing, it is stirred and treated in a water bath at 50 - 65 °C for 24 - 48 h.

4. The preparation method according to claim 3, characterized in that, In step (3), the residual sodium sulfide component in the solution is removed by dialysis, the molecular weight of the dialysis bag is 6 - 14 KDa, and the dialysis time is 2 - 5 days; the dialysis solution is centrifuged or filtered to remove insoluble impurities, and the centrifugation parameters are 8000 - 12000 rmp and the time is 5 - 10 min; The aqueous egg membrane solution is obtained as egg membrane powder through centrifugation or freeze-drying process; when using the freeze-drying process, transfer the aqueous egg membrane solution to a container, cool it at -70 ~ -80 °C for 12 - 24 h, and then transfer it to a freeze-dryer for treatment for 24 - 48 h to obtain freeze-dried water-soluble egg membrane powder.

5. The preparation method according to claim 1, characterized in that, In step (4), in the acrylation treatment of water-soluble egg membrane, the mass concentration of the prepared aqueous egg membrane solution is 2% - 8%, and the volume concentration of the added methacrylic anhydride is 0.2 - 2%; the light-shielded reaction time is 2 - 6 h, and the reaction temperature is 30 - 45 °C; the residual methacrylic anhydride component in the solution is removed by dialysis, the molecular weight of the dialysis bag is 6 - 14 KDa, and the dialysis time is 2 - 5 days.

6. The preparation method according to claim 1, wherein In step (5), the photoinitiator is selected from lithium phenyl(2,4,6-trimethylbenzoyl)phosphate (LAP) or benzophenone, The mass ratio of the GE lyophilized powder to the photoinitiator is 400:1, and the photoinduction condition is 405 nm blue light treatment for 70 - 100 s, The efficacy factors include anti-inflammatory factors, topical recombinant human epidermal growth factor, and recombinant human granulocyte macrophage-stimulating factor.

7. A hydrogel based on egg membrane components, characterized in that, It is prepared by the method according to any one of claims 1 - 6.

8. Use of the hydrogel based on egg membrane components according to claim 7 in the preparation of skin wound dressings or tissue regeneration filling materials.

9. A bioactive composite material, characterized in that, The hydrogel based on egg membrane components according to claim 7 is used as a substrate for loading bioactive components.

10. A biomedical material product, characterized in that, The hydrogel based on egg membrane components according to claim 7 is used as a raw material to prepare nanoparticles or nanofibers.

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