Sample processing equipment for detection

Abstract

The invention discloses sample treatment equipment for detection. The sample treatment equipment comprises a grinder, an ice bath bin, a box body, an injection assembly and a centrifugal assembly, the grinder is installed on the top of the box body, and the ice bath bin is arranged around the grinder. The grinder is provided with a sealing structure, a drainage cover is arranged at the bottom of the grinder, and a filter plate is installed above the drainage cover. An electromagnetic valve is arranged at the bottom end of the drainage cover; and an outlet of the electromagnetic valve is connected with the injection assembly through a conveying pipe. The interior of the box body is divided into a lower refrigeration bin through a partition plate, and the centrifugal assembly is inserted into and installed in the refrigeration bin through a window in the side wall of the box body. The injection assembly is located in the space above the partition plate and extends downwards through a positioning ring on the partition plate. Comprehensive treatment of plant tissues can be realized, the method comprises the steps of ice-bath grinding, ice-bath extraction, filtration, transfer, vacuum tube filling, refrigeration centrifugation and the like, multiple times of sample transfer or equipment replacement is not needed in the whole process, and multiple samples can be treated at the same time.

Description

Technical Field

[0001] The present invention relates to a plant sample processing device, and particularly to a sample processing device for detection. Background Art

[0002] Before detecting biological enzymes, it is necessary to preprocess plant tissue samples. Generally, this process includes steps such as ice bath grinding, cold extraction after grinding, filtration separation, and cold centrifugation. These steps require the use of multiple devices and processes, significantly increasing the complexity of the experiment and reducing the detection efficiency. In addition, the sample is prone to contamination during multiple transfer processes. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide a sample processing device for detection to solve the technical problems in the above background art.

[0004] To solve the above technical problems, the technical solution of the present invention is as follows:

[0005] A sample processing device for detection includes a grinder, an ice bath chamber, a box body, an injection assembly, and a centrifugation assembly; the grinder is installed on the top of the box body, and the ice bath chamber is arranged around the grinder; the grinder is a sealed structure, and a drainage cover is provided at its bottom, and a filter plate is installed on the upper side of the drainage cover; the bottom end of the drainage cover is installed with a solenoid valve, and the outlet of the solenoid valve is connected to the injection assembly through a delivery pipe; the lower side inside the box body is divided into a cold storage chamber by a partition, and the centrifugation assembly is installed in the cold storage chamber by inserting through a window on the side wall of the box body; the injection assembly is located in the space above the partition and descends through a positioning ring on the partition.

[0006] Further, the ice bath chamber is filled with a refrigerant, and both the cold storage chamber and the ice bath chamber are refrigerated by a semiconductor refrigeration assembly installed on the side wall.

[0007] Further, the grinder includes a grinding tank, a cover body, a driving motor, and a grinding assembly. The motor is installed at the center of the top of the cover body, and the grinding assembly is located below the cover body and is connected to the driving motor.

[0008] Further, the grinding assembly is composed of multiple layers of cutting knives installed on a rotating shaft and several grinding bars attached to the peripheral wall of the grinding tank. The grinding bars are connected to the ends of the cutting knives, and several grinding grooves are provided on the inner peripheral wall of the grinding tank.

[0009] Further, the injection assembly includes a cylinder and an injection needle. The cylinder is fixed upside down on the lower side of the top wall of the box body. A swivel joint is installed at the root of the injection needle. The top end of the swivel joint is fixed to the cylinder, and the liquid inlet is connected to the delivery pipe. The centrifugation assembly uses a vacuum tube.

[0010] Further, the centrifugation assembly includes a base and a turntable. The turntable is located on the surface of the base, and the center of the turntable is connected to a stepping motor inside the base. A circle of pipe clamps is arranged at the edge of the turntable for fixing the vacuum sealing pipe.

[0011] Further, a sealing plate is arranged on the outside of the base, and a handle is also arranged on the sealing plate.

[0012] The advantages of the present invention are as follows:

[0013] The present invention realizes the comprehensive treatment of plant tissues, including steps such as ice bath grinding, ice bath extraction, filtration, transfer, vacuum tube filling, and refrigerated centrifugation, without the need to transfer samples multiple times or replace equipment. This not only significantly reduces the operation difficulty but also effectively prevents sample contamination. In addition, the invention can process multiple tubes of samples simultaneously, facilitating the execution of various bio-enzyme detections. Description of the Drawings

[0014] Figure 1 is a three-dimensional schematic diagram of the present invention;

[0015] Figure 2 is a cross-sectional view of the present invention;

[0016] Figure 3 is a schematic diagram of the cooperation between the injection assembly and the centrifugation assembly;

[0017] Figure 4 is a cross-sectional view of the grinder.

[0018] In the figure, 1 - grinder, 11 - grinding tank, 111 - drainage cover, 112 - grinding groove, 12 - solenoid valve, 121 - delivery pipe, 13 - cover body, 14 - drive motor, 15 - rotating shaft, 16 - cutting knife, 17 - grinding bar, 2 - ice bath chamber, 3 - semiconductor refrigeration assembly, 4 - box body, 41 - partition board, 411 - positioning ring, 42 - slide rail, 5 - injection assembly, 51 - cylinder, 52 - adapter, 53 - injection needle, 6 - centrifugation assembly, 61 - base, 62 - turntable, 63 - pipe clamp, 64 - vacuum tube, 65 - sealing plate, 651 - handle, 7 - PLC controller. Detailed Embodiments

[0019] The following further describes the detailed embodiments of the present invention in conjunction with the drawings. It should be noted here that the description of these embodiments is for helping to understand the present invention, but does not constitute a limitation to the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0020] As shown in the figure:

[0021] A sample processing device for detection, comprising a grinder 1, an ice bath chamber 2, a box body 4, an injection assembly 5 and a centrifugation assembly 6; the grinder 1 is installed on the top of the box body 4, and the ice bath chamber 2 is arranged around the grinder 1; the grinder 1 is of a sealed structure, and a drainage cover 111 is provided at its bottom, and a filter plate is installed on the upper side of the drainage cover 111; a solenoid valve 12 is installed at the bottom end of the drainage cover 111, and the outlet of the solenoid valve 12 is connected to the injection assembly 5 through a delivery pipe 121; the lower side inside the box body 4 is divided into a refrigeration chamber by a partition plate 41, and the centrifugation assembly 6 is installed in the refrigeration chamber by being inserted through a window on the side wall of the box body 4 (in order to make the cooling of the centrifugation assembly 6 uniform, the bottom of the window can be appropriately raised and supported by a slide rail 42); the injection assembly 5 is located in the space above the partition plate 41 and descends through a positioning ring 411 on the partition plate 41.

[0022] As one of the optimized designs, the ice bath chamber 2 is filled with a refrigerant, and both the refrigeration chamber and the ice bath chamber 2 are refrigerated by a thermoelectric refrigeration module 3 installed on the side wall. The thermoelectric refrigeration module 3 has the advantages of a compact volume, no rotating parts and mechanical losses. Moreover, the required working temperatures of both the ice bath chamber 2 and the refrigeration chamber are generally around 2-4 degrees Celsius. Therefore, the thermoelectric refrigeration module 3 can fully meet the requirements.

[0023] For the convenience of feeding and cleaning, the grinder 1 includes a grinding tank 11, a cover body 13, a driving motor 14 and a grinding assembly. The motor is installed at the center of the top of the cover body 13, and the grinding assembly is located below the cover body 13 and is connected to the driving motor 14. In this structure, the cover body 13, the motor and the grinding assembly can be separated from the grinding tank 11 synchronously, which is beneficial to cleaning the grinding tank 11.

[0024] The grinder 1 is mainly used for crushing plant tissues. The grinding assembly can be composed of multiple layers of cutting knives 16 installed on a rotating shaft 15 and several grinding bars 17 attached to the peripheral wall of the grinding tank 11. The grinding bars 17 are connected to the ends of the cutting knives 16, and several grinding grooves 112 are formed on the inner peripheral wall of the grinding tank 11. In this way, the multiple layers of cutting knives 16 can cut the plant leaves into pieces, and then the grinding bars 17 cooperate with the grinding grooves 112 to completely crush them and release the biological enzymes in the plants.

[0025] The main function of the injection assembly 5 is to quantitatively disperse and inject the liquid obtained by grinding and leaching into the vacuum tube 64. To reduce the structural complexity and save control costs as much as possible, the injection assembly 5 includes a cylinder 51 and an injection needle 53. The cylinder 51 is fixedly installed upside down on the lower side of the top wall of the box body 4. A swivel joint 52 is installed at the root of the injection needle 53. The top end of the swivel joint 52 is fixed to the cylinder 51, and the liquid inlet is connected to the delivery pipe 121. The centrifugation assembly 6 uses a vacuum tube 64. In this way, when the solenoid valve 12 is opened, the leaching solution will slowly flow to the position of the injection needle 53. The vacuum tube 64 is a vacuum-sealed tube. When the injection needle 53 is inserted into it, the leaching solution will be automatically drawn, and the function of automatic quantification can be achieved. Of course, this requires the cooperation of the centrifugation assembly 6. Therefore, the centrifugation assembly 6 includes a base 61 and a turntable 62. The turntable 62 is located on the surface of the base 61. The center of the turntable 62 is connected to a stepper motor inside the base 61. A circle of pipe clamps 63 is arranged on the edge of the turntable 62 to fix the vacuum-sealed tube. In this way, it is only necessary for the injection assembly 5 to be located above one of the vacuum tubes 64. After each injection is completed, the stepper motor rotates according to the set parameters to switch the next vacuum tube 64 below the injection assembly 5 until all the vacuum tubes 64 are injected. Then, the centrifugation program can be started. Since the centrifugation process also needs to be carried out at a low temperature, and the centrifugation assembly 6 is inserted through a window outside the box body 4, a sealing plate 65 is arranged on the outside of the base 61 to increase airtightness. At the same time, a handle 651 is also arranged on the sealing plate 65 to facilitate the extraction of the centrifugation assembly 6 for inserting or removing the vacuum tube 64.

[0026] The working process of the present invention: Taking the determination of the activity of tea polyphenol oxidase as an example:

[0027] Before grinding, start cooling the ice bath chamber 2 first. When the temperature drops to 2-4 degrees Celsius, an appropriate amount of fresh tea leaves can be added to the grinding bowl. According to the solid-liquid ratio of 1:2, add a citric acid-phosphate buffer solution (0.1 mol / L) containing 5% PVP (w / v) and with a pH of 7.2. After ice-bathing until the internal temperature is close to 2-4 degrees Celsius, grind the plant tissue in an ice bath. At this time, the grinding tank 11 is also in a pre-cooled state, and the external refrigerant can prevent the temperature from rising during the grinding process and avoid the denaturation of biological enzymes at high temperatures. After grinding is completed, stop grinding, and then continue to maintain the ice bath temperature for 12 hours for cold extraction to fully precipitate the biological enzymes.

[0028] Before the end of cold extraction, the refrigeration chamber is pre-cooled to 2-4 degrees Celsius in advance. After the cold extraction is completed, open the solenoid valve 12, and the leaching solution will flow from the delivery pipe 121 to the injection assembly 5. The filter plate in the grinder 1 will filter out tissue debris. Then, the injection assembly 5 injects the leaching solution into the vacuum tubes 64 of the centrifugation assembly 6 respectively, and then starts centrifugation at 4°C and 9000 r / min for 35 min. Take the supernatant to obtain the crude enzyme solution.

[0029] Take 200 ml of crude enzyme solution, add 200 μL of 0.1 mol / L citric acid - phosphate buffer (pH 6) to 1.2 ml of the mixed reaction solution (0.1 mol / L citric acid - phosphate buffer: 0. proline: 1% catechol = 10:2:3), react for 30 min (in a 37°C constant temperature water bath), terminate the reaction with 1.2 ml of 6 mol urea, and measure the absorbance at a wavelength of 460 nm. The control is the reaction mixture without catechol.

[0030] Enzyme activity unit: Under the conditions of this experiment, an increase in the absorbance value of the catechol reaction solution at 460 nm by 0.01 per minute is defined as one activity unit.

[0031] It should be noted that in order to reduce the control difficulty, the present invention uses a PLC controller 7 for integrated control. To avoid the heat dissipation affecting the cooling inside the box 4, it is installed separately outside the box 4. The main automatic control process is the cooperation between the cylinder 51 of the injection component 5 and the stepping motor, and the rest can be controlled by manual input.

[0032] The above - mentioned embodiments of the present invention have been described in detail in conjunction with the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, without departing from the principles and spirit of the present invention, various changes, modifications, substitutions, and variations made to these embodiments still fall within the protection scope of the present invention.

Claims

1. A sample processing device for detection, characterized in that: It includes a grinder, an ice bath chamber, a box body, an injection assembly and a centrifugal assembly; the grinder is installed on the top of the box body, and the ice bath chamber is arranged around the grinder; the grinder is a sealed structure, and a drainage cover is provided at its bottom, and a filter plate is installed on the upper side of the drainage cover; a solenoid valve is installed at the bottom end of the drainage cover, and the outlet of the solenoid valve is connected to the injection assembly through a delivery pipe; the lower side inside the box body is divided into a refrigeration chamber by a partition board, and the centrifugal assembly is installed in the refrigeration chamber by inserting through a window on the side wall of the box body; the injection assembly is located in the space above the partition board and descends through a positioning ring on the partition board.

2. The sample processing device for detection according to claim 1, wherein: The ice bath chamber is filled with a refrigerant, and both the refrigeration chamber and the ice bath chamber are refrigerated by a semiconductor refrigeration assembly installed on the side wall.

3. The sample processing device for detection according to claim 1, characterized in that: The grinder includes a grinding tank, a cover body, a driving motor and a grinding assembly. The motor is installed at the center of the top of the cover body, and the grinding assembly is located below the cover body and is connected to the driving motor.

4. The sample processing device for detection according to claim 1, characterized in that: The grinding assembly consists of multiple layers of cutting knives installed on a rotating shaft and several grinding bars attached to the peripheral wall of the grinding tank. The grinding bars are connected to the ends of the cutting knives, and several grinding grooves are provided on the inner peripheral wall of the grinding tank.

5. The sample processing device for detection according to claim 1, wherein: The injection assembly includes a cylinder and an injection needle. The cylinder is fixed upside down under the top wall of the box body. A swivel joint is installed at the root of the injection needle. The top end of the swivel joint is fixed to the cylinder, and the liquid inlet is connected to the delivery pipe. The centrifugal assembly uses a vacuum tube.

6. The sample processing device for detection according to claim 5, wherein: The centrifugal assembly includes a base and a turntable. The turntable is located on the surface of the base, and the center of the turntable is connected to a stepping motor inside the base; a circle of pipe clamps is provided on the edge of the turntable for fixing the vacuum sealing pipe.

7. The sample processing device for detection according to claim 6, characterized in that: A sealing plate is provided on the outside of the base, and a handle is also provided on the sealing plate.

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