Collagen identification method

Abstract

The invention discloses an identification method of collagen. A reagent for identification is genipin. The method comprises the following identification steps: I, when the concentration of the collagen is higher than 0.05%, uniformly mixing the genipin solution with the collagen and / or the collagen product solution for reaction and color development, and directly comparing colors for identification; iI, when the concentration of the collagen is lower than 0.05%, after a collagen product solution is extracted, developing by using a genipin solution, and directly comparing colors for identification; and III, when the concentration of the collagen is lower than 0.05%, uniformly mixing the genipin solution and the collagen solution for reaction and color development, and directly comparing colors for identification. According to the identification method, the requirement for the range of the collagen content in collagen raw materials and products is wider, expensive equipment does not need to be used in the identification process, and the identification cost is greatly reduced; the operation process is convenient, the experiment period is short, the identification speed is high, the sensitivity is higher, the stability is good, and the collagen content identification efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technologies, and particularly to a method for identifying collagen. Background Art

[0002] As a key biological macromolecule, collagen has wide applications in biomedicine, cosmetology, and the food industry. Accurately identifying and detecting collagen raw materials and their products is an important link to ensure product quality and safety. With the rapid development of the collagen-related product market, higher requirements are put forward for the identification and detection methods of collagen, including the simplicity, accuracy, and sensitivity of detection.

[0003] Currently, the identification and detection methods of collagen mainly include polyacrylamide gel electrophoresis (isoelectric point method), peptide mapping, terminal amino acid sequence detection, isoelectric focusing electrophoresis, etc. Although these methods can achieve the identification of collagen to a certain extent, each has certain limitations. For example, peptide mapping and terminal amino acid sequence detection rely on expensive equipment such as high-performance liquid chromatographs and amino acid sequence analyzers, and the operations are complex and the costs are high. Although isoelectric focusing electrophoresis does not require expensive equipment, its detection sensitivity is limited by the collagen content in the sample, and concentration methods need to be used to improve the detection sensitivity, which increases the complexity of the operation and the requirements for technicians. Summary of the Invention

[0004] In the prior art, collagen at a concentration of 0.01% cannot be detected by different instruments. Especially for products such as gels and creams, the collagen concentration is very low, making it difficult to detect; the composition of creams is relatively complex, with at least 7 - 9 components. Especially after emulsification, the collagen component is very low (cannot be fully mixed), so it is difficult to identify; the characteristic of gels is that they are very thick and cannot be directly identified. Especially when detecting creams and gel products, a series of steps such as dilution - suction filtration - filtration - concentration are required to extract collagen. However, after such steps, the collagen concentration of the product becomes even lower, making it even more impossible to quickly detect.

[0005] To address the above problems, the present invention intends to provide a new method for identifying collagen, and the method has a wider range requirement for the collagen content in collagen raw materials and products.

[0006] The present invention provides a method for identifying collagen, and the reagent for identification is genipin; the identification steps are selected from:

[0007] I. When the collagen concentration is higher than 0.05%, after mixing the genipin solution with the collagen and / or collagen product solution and reacting to develop color, directly compare the colors for identification;

[0008] II. When the collagen concentration is lower than 0.05%, after the collagen product solution is extracted, it is colored with genipin solution, and the color is directly compared for identification.

[0009] III. When the collagen concentration is lower than 0.05%, after the genipin solution and the collagen solution are mixed and reacted to develop color, the color is directly compared for identification.

[0010] In some specific embodiments of the present invention, the collagen is selected from type I collagen, type II collagen, type III collagen, type IV collagen, type V collagen, type VII collagen, type VIII collagen, and type XVII collagen.

[0011] During the research process, the inventor accidentally found that the color development of the reaction between genipin and collagen is related to the molecular weight of collagen. Among them, the molecular weights of the collagens used in Examples 1 to 15 are all between 40 and 50 KB, and all of their color developments are blue; the molecular weight of T8 collagen in Example 16 is 15 KB, and its color development is yellow.

[0012] In the present invention, when the molecular weight of collagen is 10 - 20 KB, the color development is yellow; when the molecular weight of collagen is 40 - 50 KB, the color development is blue; further, the blue includes: powder blue, sky blue, light blue, sea blue, navy blue, peacock blue, and blue - gray.

[0013] In some specific embodiments of the present invention, when the collagen is T8, the color development is yellow. T8 is the recombinant type III humanized collagen of Jinbo, which is disclosed in ZL202311272338.8, and the sequence is as follows:

[0014] GERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAPGERGAPGFRGPAGPNGIPGEKGPAGERGAP

[0015] In the present invention, the collagen includes: pure collagen, collagen products, and collagen peptides;

[0016] In some specific embodiments of the present invention, the collagen products include: vaginal gel, composite vaginal gel, anti-HPV gel, lubricant, composite lubricant, recombinant collagen care ointment, recombinant collagen sterile repair dressing, recombinant collagen sterile repair solution, eye drops.

[0017] In some specific embodiments of the present invention, the concentration of genipin is not less than 0.1%.

[0018] In some specific embodiments of the present invention, the concentration of genipin is 0.1% - 1%.

[0019] In some specific embodiments of the present invention, the reaction is carried out under alkaline conditions.

[0020] The present invention also provides a collagen identification kit, and the kit includes genipin.

[0021] In the present invention, the term "higher than" means equal to or greater than a certain value; the term "lower than" means less than or equal to a certain value.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] The identification method of the present invention has a wider range requirement for the collagen content in collagen raw materials and products (protein content of 0.01% can also be identified);

[0024] 1. Expensive equipment is not required during the identification process, which greatly reduces the identification cost; the operation process is relatively convenient, the experimental period is short, the identification speed is fast, the sensitivity is higher, and the stability is good, improving the efficiency of collagen content identification;

[0025] 2. It has wide applicability: different types of collagen (I, II, III, Ⅳ, V, VII, VIII, XVII), recombinant collagen vaginal gel, anti-HPV gel, recombinant collagen lubricant, freeze-dried powder products, etc. can all be detected;

[0026] 3. By comparing colors, different types of proteins with the same concentration can be identified, and proteins with different concentrations of the same type can also be identified according to colors. Description of the Drawings

[0027] Figure 1 Type I collagen with different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g; g: water or PBS solution);

[0028] Figure 2 Type II collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g);

[0029] Figure 3 Type III collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g; g: water or PBS solution);

[0030] Figure 4 Type IV collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g);

[0031] Figure 5 Type V collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g);

[0032] Figure 6 Type VII collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g);

[0033] Figure 7 Type VIII collagen at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g);

[0034] Figure 8Collagen type XVII at different concentrations (a: collagen concentration is 1 mg / g; b: collagen concentration is 0.8 mg / g; c: collagen concentration is 0.6 mg / g; d: collagen concentration is 0.4 mg / g; e: collagen concentration is 0.2 mg / g; f: collagen concentration is 0.1 mg / g; g: water or PBS solution);

[0035] Figure 9 shows the reaction of vaginal gel / composite vaginal gel with genipin (9-A is 0.05% collagen type XVII; 9-B is 0.005% collagen type XVII + 0.05% collagen type III);

[0036] Figure 10 The reaction of anti-HPV gel (0.05% collagen type III + 0.06% JB) with genipin;

[0037] Figure 11 The reaction of lubricant / composite lubricant with genipin (01 and 02 on the left are 0.05% collagen type III; 01 and 02 on the right are 0.06% JB + 0.05% collagen type III);

[0038] Figure 12 The reaction of recombinant collagen care ointment (0.01% collagen type III) with genipin;

[0039] Figure 13 The reaction of recombinant collagen sterile solution (0.01% collagen type III) with genipin;

[0040] Figure 14 The reaction of recombinant collagen sterile repair solution (0.01% collagen type I) with genipin;

[0041] Figure 15 The color development of genipin on eye drops;

[0042] Figure 16 The color development of genipin on T8 protein (collagen type III) (PH: 5.0);

[0043] Figure 17 The color development of genipin on T8 protein (collagen type III) (PH: 7.2);

[0044] Figure 18 The color development of genipin on T8 protein (collagen type III) (PH: neutral);

[0045] Figure 19 The colors of different types of proteins (taking the same concentration of 1 mg / g and the same reaction time as an example);

[0046] Figure 20 is the color of the same type of protein (taking different concentrations and the same reaction time as an example);

[0047] Figure 21 is a color comparison card. Specific implementation manner

[0048] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0049] Example 1 Type I collagen

[0050] Weigh Type I collagen and prepare protein solutions with concentrations of 1mg / g, 0.8mg / g, 0.6mg / g, 0.4mg / g, 0.2mg / g, and 0.1mg / g using PBS solution or purified water. Take 5g of each of the 6 gradient concentration solutions, add 5ml of genipin solution (concentration 1%) or 0.05g of genipin solid, and mix well to make them react.

[0051] The results are as Figure 1 shown. After standing at room temperature for 17h, the color reaction of protein solutions with a concentration of 0.2mg / g or more with genipin is relatively obvious, and the color reaction of protein solutions with a concentration of 0.2mg / g or less is relatively weak.

[0052] Example 2 Type II collagen

[0053] Weigh Type II collagen and prepare protein solutions with concentrations of 1mg / g, 0.8mg / g, 0.6mg / g, 0.4mg / g, 0.2mg / g, and 0.1mg / g using PBS solution or purified water. Take 5g of each of the 6 gradient concentration solutions, add 5ml of genipin solution (concentration 1%) or 0.05g of genipin solid, and mix well to make them react.

[0054] The results are as Figure 2 shown. After standing at room temperature for 17h, the color reaction of protein solutions with a concentration of 0.4mg / g or more with genipin is relatively obvious, and the color reaction of protein solutions with a concentration of 0.4mg / g or less is relatively weak.

[0055] Example 3 Type III collagen

[0056] Weigh type III collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to make them react.

[0057] The results are as Figure 3 shown. After standing at room temperature for 17 h, the color reaction of protein solutions with concentrations above 0.2 mg / g with genipin is relatively obvious, while the color reaction of protein solutions with concentrations of 0.2 mg / g and below is relatively weak.

[0058] Example 4 Type IV collagen

[0059] Weigh type IV collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to make them react.

[0060] The results are as Figure 4 shown. After standing at room temperature for 17 h, the color reaction of protein solutions with concentrations above 0.6 mg / g with genipin is relatively obvious, while the color reaction of protein solutions with concentrations of 0.6 mg / g and below is relatively weak.

[0061] Example 5 Type V collagen

[0062] Weigh type V collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to make them react.

[0063] The results are as Figure 5 shown. After standing at room temperature for 17 h, the color reaction of protein solutions with concentrations above 0.6 mg / g with genipin is relatively obvious, while the color reaction of protein solutions with concentrations of 0.6 mg / g and below is relatively weak.

[0064] Example 6 Type VII collagen

[0065] Weigh type VII collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to allow the reaction to occur.

[0066] The results are as Figure 6 shown. After standing at room temperature for 17 h, the color reaction between the protein solutions with a concentration above 0.4 mg / g and genipin is relatively obvious, while the color reaction of the protein solutions with a concentration of 0.4 mg / g and below is relatively weak.

[0067] Example 7 Type VIII collagen

[0068] Weigh type VIII collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to allow the reaction to occur.

[0069] The results are as Figure 7 shown. After standing at room temperature for 17 h, the color reaction between the protein solutions with a concentration above 0.6 mg / g and genipin is relatively obvious, while the color reaction of the protein solutions with a concentration of 0.6 mg / g and below is relatively weak.

[0070] Example 8 Type XVII collagen

[0071] Weigh type XVII collagen and prepare protein solutions with concentrations of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g using PBS solution or purified water. Take 5 g of each of the 6 gradient concentration solutions, add 5 ml of genipin solution (concentration 1%) or 0.05 g of genipin solid, and mix well to allow the reaction to occur.

[0072] The results are as Figure 8 shown. After standing at room temperature for 17 h, the color reaction between the protein solutions with a concentration above 0.6 mg / g and genipin is relatively obvious, while the color reaction of the protein solutions with a concentration of 0.2 mg / g and below is relatively weak.

[0073] Example 9 Vaginal gel (0.05% type XVII collagen) / Composite vaginal gel (0.005% type XVII collagen + 0.05% type III collagen)

[0074] Weigh 2 g each of three batches or two batches of vaginal gel (type XVII collagen) / compound vaginal gel (type XVII + type III collagen) and the blank control, add 2 ml of genipin solution (1%), and mix well to allow reaction.

[0075] The results are as Figure 9-A / Figure 9-B shown. After standing at room temperature for 12 h, the color of the product is blue, and the blank control does not show color.

[0076] Example 10 Anti-HPV Gel (0.06% JB + 0.05% type III collagen)

[0077] Weigh 2 g each of three batches or two batches of anti-HPV gel and the blank control, add 2 ml of genipin solution (0.01%), and mix well to allow reaction.

[0078] As Figure 10 shown. After standing at room temperature for 12 h, the color of the product is blue, and the blank control does not show color.

[0079] Example 11 Lubricant (0.05% type III collagen) / Compound Lubricant (0.06% JB + 0.05% type III collagen)

[0080] Weigh 2 g each of three batches or two batches of lubricant (type III collagen) / compound lubricant (JB + type III collagen) blank control, add 2 ml of genipin solution (1%), and mix well to allow reaction. After standing at room temperature for 12 h, the color of the product is blue, and the blank control does not show color.

[0081] The results are as Figure 11 shown. After standing at room temperature for 12 h, the color of the product is blue, and the blank control does not show color.

[0082] Example 12 Recombinant Collagen Nursing Ointment (0.01% type III collagen)

[0083] Weigh 5 g each of three batches of recombinant collagen nursing ointment and the blank control. After diluting the samples, filter them by suction to extract the protein. The dilution volumes are as follows:

[0084] Recombinant collagen nursing ointment + water (5 g → 50 g)

[0085] Blank control + water (5 g → 50 g)

[0086] Filter by suction using a suction filter, evaporate the filtrate overnight in an oven at 80 °C, concentrate it to 6 mL, add 0.06 g of genipin powder after restoring to room temperature to make the final concentration of genipin 1%, and mix well to allow reaction. Observe after standing at room temperature for 19 h. The color reaction of the filtrates of the three batches with genipin is relatively obvious, and there is no color change in the blank group.

[0087] As Figure 12 shown, after observing after standing at room temperature for 19 h, the color reaction of the filtrates of the three batches with genipin was relatively obvious, and there was no color change in the blank group.

[0088] Example 13 Recombinant Collagen Sterile Solution (0.01% Type III Collagen)

[0089] Weigh 5 g each of the recombinant collagen sterile solutions of three batches and the blank control. After diluting the samples, filter them by suction to extract the protein. The dilution volumes are as follows:

[0090] Recombinant Collagen Sterile Solution + Water (5 g → 50 g)

[0091] Blank Control + Water (5 g → 50 g)

[0092] Filter by suction using a suction filter. Evaporate the filtrate overnight in an oven at 80 °C until it is concentrated to 6 mL. After returning to room temperature, add 0.06 g of genipin powder to make the final concentration of genipin 1%, mix well to allow the reaction. After standing at room temperature for 19 h and observing, the color reaction of the filtrates of the three batches with genipin was relatively obvious, and there was no color change in the blank group.

[0093] As Figure 13 shown, after observing after standing at room temperature for 19 h, the color reaction of the filtrates of the three batches with genipin was relatively obvious, and there was no color change in the blank group.

[0094] Example 14 Recombinant Collagen Sterile Repair Solution (0.01% Type I Collagen)

[0095] Weigh 5 g each of the recombinant collagen sterile repair solutions of three batches and the blank control. After diluting the samples, filter them by suction to extract the protein. The dilution volumes are as follows:

[0096] Recombinant Collagen Sterile Repair Solution + Water (5 g → 50 g)

[0097] Blank Control + Water (5 g → 50 g)

[0098] Filter by suction using a suction filter. Evaporate the filtrate overnight in an oven at 80 °C until it is concentrated to 6 mL. After returning to room temperature, add 0.06 g of genipin powder to make the final concentration of genipin 1%, mix well to allow the reaction. After standing at room temperature for 19 h and observing, the color reaction of the filtrates of the three batches with genipin was relatively obvious, and there was no color change in the blank group.

[0099] As Figure 14 shown, after observing after standing at room temperature for 19 h, the color reaction of the filtrates of the three batches with genipin was relatively obvious, and there was no color change in the blank group.

[0100] Example 15 Color Development of Genipin on Eye Drops

[0101] Weigh 1 g of eye drops (0.05% type III collagen, 5 batches, 08240101 accelerated at 55 °C for 80 days, 08240101 at room temperature, 08240501, 08240502, 08240503) with different batches and samples under different conditions, as well as 1 g of blank control. Add 1 ml of genipin solution (5%) and mix well to make them react.

[0102] It was observed through electrophoresis experiments that part of the protein was degraded when the eye drops were accelerated at 55 °C for 80 days, and there was also degradation under other conditions. After adding genipin, the colors of the samples all changed to blue, while the color of the blank control did not change ( Figure 15 ), so this method can be used to identify the degraded protein.

[0103] Example 16 T8(III) protein

[0104] I. Experimental method

[0105] Weigh 100 mg of T8 (the molecular weight of T8 is 15 KB), and make up to 100 g with PBS (or water) to prepare a protein stock solution of 1 mg / g. Take 4 g, 3 g, 2 g, 1 g, and 0.5 g of the protein stock solution (1 mg / g) in turn, and make up to 5 g with PBS in turn to prepare gradient solutions with concentrations of 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, and 0.1 mg / g: Take 5 g of each of the 6 gradient concentration solutions, add 0.05 g of genipin solid (to make the final concentration of genipin 1%), and mix well to make them react.

[0106] II. Experimental results

[0107] 1. T8 (gradient solutions of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, 0.1 mg / g (prepared with PBS (pH: 5.0 PBS)) + genipin powder (final concentration 1%)

[0108] As Figure 16 shown, after adding genipin powder (the final concentration of genipin is 1%), the protein solutions with 6 concentration gradients all reacted with genipin to show color. The protein solutions with concentrations above 0.2 mg / g showed obvious color development, and the protein solution of 0.1 mg / g showed weaker color development.

[0109] 2. T8 (gradient solutions of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, 0.1 mg / g (prepared with PBS (pH: 7.2 PBS)) + genipin powder (final concentration 1%)

[0110] As Figure 17As shown, after adding genipin powder (final concentration of genipin is 1%), protein solutions with 6 concentration gradients all underwent a color reaction with genipin, and the color changed from orange-yellow to brown. Protein solutions with a concentration of 0.2 mg / g or higher showed obvious color development, while the protein solution with a concentration of 0.1 mg / g showed weaker color development.

[0111] 3. T8 (gradient solutions (prepared with water) of 1 mg / g, 0.8 mg / g, 0.6 mg / g, 0.4 mg / g, 0.2 mg / g, 0.1 mg / g, pH: neutral) + genipin powder (final concentration 1%)

[0112] As Figure 18 shown, after adding genipin powder (final concentration of genipin is 1%), protein solutions with a concentration of 0.2 mg / g or higher showed obvious color development, while the protein solution with a concentration of 0.1 mg / g did not show any color change.

[0113] In summary, pH affects the color development of T8 protein. From acidic - neutral - alkaline, the orange-yellow color reaction gradually becomes more obvious.

[0114] The present invention can, through the color reaction of genipin and by comparing colors, identify proteins of different types at the same concentration, and at the same time can also identify proteins of the same type at different concentrations based on the color.

[0115] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for identifying collagen, characterized in that, The reagent for identification is genipin; the identification steps are selected from: I. When the collagen concentration is higher than 0.05%, after mixing the genipin solution with the collagen and / or collagen product solution and reacting to develop color, directly compare the colors for identification; II. When the collagen concentration is lower than 0.05%, after extracting the collagen product solution, use the genipin solution to develop color and directly compare the colors for identification; III. When the collagen concentration is lower than 0.05%, after mixing the genipin solution with the collagen solution and reacting to develop color, directly compare the colors for identification.

2. The identification method according to claim 1, wherein The collagen is selected from type I collagen, type II collagen, type III collagen, type IV collagen, type V collagen, type VII collagen, type VIII collagen, and type XVII collagen.

3. The identification method according to claim 1, wherein When the molecular weight of the collagen is 10 - 20 KB, the color development is yellow; when the molecular weight of the collagen is 40 - 50 KB, the color development is blue; further, the blue includes: powder blue, sky blue, light blue, sea blue, navy blue, peacock blue, and blue - gray.

4. The identification method according to claim 3, characterized in that, When the collagen is T8, the color development is yellow; further, when the reaction is carried out under alkaline conditions, the color development is orange - yellow.

5. The identification method according to claim 1, characterized in that, The collagen includes: pure collagen, collagen products, collagen peptides, and recombinant humanized collagen.

6. The identification method according to claim 5, characterized in that, The collagen products include: vaginal gels, compound vaginal gels, anti - HPV gels, lubricants, compound lubricants, recombinant collagen care ointments, recombinant collagen sterile repair dressings, recombinant collagen sterile repair solutions, and eye drops.

7. The identification method according to claim 1, characterized in that The concentration of the genipin is not less than 0.1%.

8. The identification method according to claim 1, wherein, The concentration of the genipin is 0.1% - 1%.

9. The identification method according to claim 1, characterized in that, The identification time is not less than 7.5 hours.

10. A collagen identification kit, characterized in that, The kit includes genipin.

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