Stock solution screening and filtering device for preparing bio-enzyme preparation

Abstract

The invention discloses a stock solution screening and filtering device for preparing a biological enzyme preparation, and relates to the technical field of screening and filtering devices. The device comprises an outer cylinder, an inner cylinder is arranged in the outer cylinder, filtering holes are formed in the bottom of the inner cylinder, a rotating shaft is arranged in the inner cylinder, the two ends of the rotating shaft penetrate through the inner cylinder, the surface of the rotating shaft is fixedly connected with the inner side face of the inner cylinder, and the ends, close to each other, of two torsion rods are fixedly connected with the two ends of the rotating shaft correspondingly; the surface of the driving assembly is in limiting sliding connection with the inner side face of the outer barrel, the ends, away from each other, of the two torsion bars are fixedly connected to the surface of the driving assembly, and when the outer barrel and the inner barrel synchronously rotate, due to the fact that the torsion bars are spiral and are uneven in stress during rotation, the inner barrel can irregularly shake; and the bottom of the inner cylinder irregularly swings in the clear liquid in the outer cylinder, so that sediments can be swung away from the interiors of the filter holes, the sediments are prevented from being continuously accumulated in the filter holes under the action of centrifugal force, and the filter holes are prevented from being blocked.

Description

Technical Field

[0001] The present invention relates to the technical field of screening devices, and particularly relates to a stock solution screening device for the preparation of biological enzyme preparations. Background Art

[0002] Biological enzyme preparations are products prepared by using biotechnology to extract enzymes from organisms and adding cofactors, stabilizers, carriers, etc. The core component is enzyme protein, which has the characteristics of high efficiency, specificity, mildness, adjustability, environmental protection, etc. In the pharmaceutical industry, it can be used to prepare pharmaceutical intermediates, bulk drugs, etc. In the food industry, it can be used for bread making, beer brewing, cheese production, etc. In the textile industry, it is used for desizing, washing and finishing, etc. In the paper industry, it is used for raw material pretreatment and improving paper properties. In the feed industry, it can improve the digestion and absorption rate of nutrients and reduce phosphorus emissions. In the field of environmental protection, it can treat wastewater and repair soil; The stock solution screening of biological enzyme preparations refers to the filtration and screening operation of the original fermentation broth or extract containing the target enzyme during the production of biological enzyme preparations, aiming to remove cell debris, unreacted substrates, miscellaneous proteins and other solid impurities in the stock solution to obtain a relatively pure and uniform enzyme solution, laying a foundation for subsequent steps such as enzyme purification and concentration. To improve the enzyme recovery rate, it is usually necessary to completely squeeze out the clear liquid in the impurities. At the same time, during the screening process, it is also necessary to maintain the environment where the enzyme is located, keep the temperature, pressure, etc. within a suitable range, and ensure the quality and activity of the biological enzyme preparation. Therefore, we propose a stock solution screening device for the preparation of biological enzyme preparations. Summary of the Invention

[0003] To solve the above technical problems, the present invention provides a stock solution screening device for the preparation of biological enzyme preparations, including: A bottom plate, on the top of which a driving mechanism is fixedly connected. An centrifugal mechanism is inserted directly above the driving mechanism, and the driving mechanism is used to drive the centrifugal mechanism to rotate and centrifuge. A shield mechanism, which is fixedly connected to the top of the bottom plate, and the inner side surface of the shield mechanism is slidably connected to the outer side surface of the driving mechanism. The top of the shield mechanism is fixedly connected with a top plate; A limiting mechanism, which is fixedly connected to the inner side surface of the shield mechanism, and the inner side surface of the limiting mechanism is slidably connected to the outer side surface of the centrifugal mechanism. The limiting mechanism is used to limit the centrifugal rotation of the centrifugal mechanism; Among them, the centrifugal mechanism includes: An outer cylinder, several of which are arranged directly above the driving mechanism, and an inner cylinder is arranged inside each of the several outer cylinders. The bottom of the inner cylinder is provided with filter holes, and the filter holes are set as long and narrow ellipses. There are several filter holes, and the several filter holes are evenly distributed at the bottom of the inner cylinder; A rotating shaft, the rotating shaft is arranged inside the inner cylinder, both ends of the rotating shaft penetrate through the inner cylinder, and the surface of the rotating shaft is fixedly connected to the inner side surface of the inner cylinder; Torsion bars, the torsion bars are symmetrically arranged at the interval between the outer cylinder and the inner cylinder, and the ends of the two torsion bars close to each other are respectively fixedly connected to both ends of the rotating shaft, and the torsion bars are arranged in a spiral shape; A driving assembly, the surface of the driving assembly is in limited sliding connection with the inner side surface of the outer cylinder, and the ends of the two torsion bars away from each other are fixedly connected to the surface of the driving assembly. The output end of the driving assembly drives the two torsion bars to rotate synchronously. The side of the driving assembly away from the outer cylinder is in rotational limit connection with the side of the top plate close to the bottom plate; Start the shield mechanism. The shield mechanism drives the top plate to rise, drives the limit mechanism, the driving assembly, and the inner cylinder to rise. The outer cylinder in limited sliding connection with the driving assembly also rises under the action of friction. Then, the outer cylinder is pulled out partially, the top of the inner cylinder is exposed, the original liquid is added into the inner cylinder, the outer cylinder is reset, and then the shield mechanism is started again. The device is reset, and the outer cylinder is reinserted into the output end of the driving mechanism. Start the driving mechanism, and the driving mechanism drives the outer cylinder, the driving assembly, and the inner cylinder to rotate, centrifuging the original liquid to precipitate impurities, bacteria, unreacted substrates, etc. in the original liquid. At this time, the inner cylinder is inside the clarified original liquid in the outer cylinder, and the precipitate is intercepted inside the inner cylinder by the filter holes. Then start the shield mechanism. The gravity of the clear liquid in the outer cylinder overcomes the friction between the driving assembly and the outer cylinder. The shield mechanism finally drives the inner cylinder to rise a certain height, and the bottom of the inner cylinder is directly above the clear liquid inside the outer cylinder. Continue to drive the outer cylinder and the inner cylinder to rotate. Under the action of centrifugation, the remaining clear liquid in the precipitate is thrown out, preliminarily drying the precipitate and avoiding the waste of enzymes in the clear liquid. The setting of the limit mechanism can limit the outer cylinder to prevent the inserted outer cylinder from detaching from the driving mechanism and avoid wear at the insertion part of the two. The long and narrow elliptical filter holes can be made finer within a limited surface area to obtain a better filtering effect. When the outer cylinder and the inner cylinder rotate synchronously, since the torsion bars are arranged in a spiral shape, the force is uneven during rotation, which will cause the inner cylinder to generate irregular shaking. The bottom of the inner cylinder swings irregularly in the clear liquid inside the outer cylinder, which can swing the precipitate away from the inside of the filter holes, prevent the precipitate from continuously accumulating inside the filter holes under the action of centrifugal force, and avoid filter hole blockage. When the precipitate gradually settles into the filter holes at the bottom of the inner cylinder due to centrifugal force, the precipitate gets stuck in the filter holes and cannot rotate with the clear liquid, causing the center of gravity of the inner cylinder to drop and be in a non-coincident state with the axis of the rotating shaft. At this time, the shaking of the inner cylinder intensifies, and the precipitate is quickly swung away in time to avoid blockage in time.

[0004] Further, a butterfly plate is arranged inside the inner cylinder. There are two butterfly plates, and one side of the two butterfly plates close to each other is rotatably connected. The rotating shaft penetrates through the connection part of the two butterfly plates, and the surface of the rotating shaft is rotatably connected with the inner side surface of the butterfly plate. The side of the butterfly plate away from the rotating shaft is serrated. The butterfly plates on both sides of the rotating shaft naturally droop under the action of gravity, which is convenient for adding the stock solution. The outermost ring of the butterfly plate close to the filter holes has a slope. During centrifugation, the butterfly plate rotates with the rotation of the rotating shaft. Under the action of centrifugal force, the two butterfly plates rotate around the rotating shaft and finally form a complete circular plate to cover the opening of the inner cylinder, avoiding the splashing of the stock solution during centrifugation. Then, the driving assembly is started. The driving assembly drives the torsion bar and the rotating shaft to rotate, and finally drives the inner cylinder to rotate 180 degrees along the axial direction of the rotating shaft. Then, the inner cylinder is driven to rotate along its axial direction again, and the two butterfly plates are flattened. When the inner cylinder rotates, the air continuously contacts the inner wall of the filter holes and gradually completely dredges the filter holes. The sediment drops onto the surface of the butterfly plate and is stuck at the slope of the butterfly plate and the inner wall of the inner cylinder under the action of centrifugal force, and the remaining clear liquid is squeezed out from the gap between the serrated part of the butterfly plate and the inner cylinder, thoroughly screening the clear liquid. Moreover, when the inner cylinder rotates, the air continuously contacts the inner wall of the filter holes and gradually completely dredges the filter holes, dredging all the filter holes that may be blocked, avoiding the need for disassembly and cleaning during the operation of the device. And the filter residue accumulates at the slope of the butterfly plate. After the machine stops, the butterfly plate cannot naturally descend, and the filter residue will not fall randomly. After preparing a container for holding the filter residue below the butterfly plate, press the butterfly plate so that it can rotate. At the same time, the filter residue is broken into large pieces. Under the action of gravity, the two butterfly plates rotate into a V shape towards the opening of the inner cylinder, which is convenient for the dropping of the large-piece filter residue, avoiding the filter residue adhering to the inner wall of the inner cylinder and being difficult to clean, and at the same time, the dropping of the large-piece dry filter residue is not likely to generate dust.

[0005] Further, a buffer rod is fixedly connected to the outer side surface of the inner cylinder. A plurality of buffer rods are evenly distributed along the circumferential direction of the inner cylinder. The buffer rod is spiral. A hemisphere is fixedly connected to the side of the buffer rod away from the inner cylinder. The side of the hemisphere away from the buffer rod is slidably connected to the inner side surface of the outer cylinder. When the inner cylinder shakes, it drives the buffer rod to move, drives the hemisphere to squeeze the inner cylinder, and the hemisphere slides on the inner wall of the outer cylinder, and the buffer rod bends. Then, under the action of its bending elasticity, the force of the inner cylinder's swing is consumed, avoiding the swing of the outer cylinder and at the same time limiting the swing amplitude of the inner cylinder.

[0006] Further, the driving assembly includes a ring body disposed directly above the outer cylinder. One side of the ring body away from the outer cylinder is inlaid on one side of the top plate close to the bottom plate, and the surface of the ring body is in limit rotational connection with the inner side surface of the top plate. A slide plate is fixedly connected to the side of the ring body away from the top plate. The slide plates are symmetrically arranged on the side of the ring body away from the top plate. One side of each of the two slide plates away from the ring body extends into the inner part of the outer cylinder. Both sides of the slide plate are in the shape of a dovetail, and the surface of the slide plate is in sliding connection with the outer cylinder. The slide plate and the outer cylinder that are in limit sliding connection through the dovetail structure can remove the outer cylinder without affecting the rotation of the slide plate along with the outer cylinder. When the outer cylinder is empty, the friction force between the outer cylinder and the slide plate is greater than the self-weight of the outer cylinder, and the outer cylinder is taken out together with the slide plate. When there is clear liquid in the outer cylinder, the friction force between the outer cylinder and the slide plate is less than the combined self-weight of the outer cylinder and the clear liquid, and the outer cylinder remains on the top of the driving mechanism for easy removal.

[0007] Further, motors are inlaid in each of the two slide plates, and the outer side surface of the motor is fixedly connected to the inner side surface of the slide plate. Rotating shafts are fixedly connected to one side of the two motors close to each other. One end of each of the two rotating shafts close to each other is fixedly connected to one end of each of the two torsion bars away from each other. A sealing plate is fixedly connected to the side of the motor close to the rotating shaft. The inner side surface of the sealing plate is in rotational connection with the surface of the rotating shaft, and the diameter of the sealing plate is larger than that of the motor. When the inner cylinder needs to be flipped, the motor is started. The output end of the motor drives the rotating shaft to rotate, driving the torsion bar to rotate. Finally, the two motors drive the two ends of the rotating shaft to rotate synchronously, driving the inner cylinder to flip. The setting of the sealing plate can prevent the clear liquid from contacting the motor, achieving the effect of protecting the motor.

[0008] Further, the driving mechanism includes a driving box fixedly connected to the top of the bottom plate. A driving disc is disposed on the side of the driving box away from the bottom plate, and the surface of the driving disc is fixedly connected to the inner side surface of the driving box. A driving rod is rotatably connected to the inner side surface of the driving disc, and the driving rod is driven to rotate by the driving box. A number of driving rods corresponding to the outer cylinder are provided. The side of the driving rod away from the driving disc is in the shape of a dovetail. A chuck is disposed on the side of the driving rod away from the driving disc, and the inner side surface of the chuck is inserted into the side of the driving rod away from the driving disc. The side of the chuck away from the driving disc is fixedly connected to the side of the outer cylinder close to the driving disc. The driving box drives the driving rod to rotate, and the driving rod drives the chuck to rotate, finally driving the outer cylinder to rotate. The inserted chuck and the driving rod can facilitate the removal of the outer cylinder.

[0009] Further, the shield mechanism includes a housing, the inner side surface of the housing is slidably connected to the outer side surface of the drive box, the side of the housing away from the bottom plate is fixedly connected to the side of the top plate close to the bottom plate, and a telescopic rod is fixedly connected to the side of the housing close to the bottom plate. The side of the telescopic rod away from the housing is fixedly connected to the top of the bottom plate. When the telescopic rod is started, the telescopic rod expands and contracts, driving the housing and the top plate to rise and fall, and completing the opening and closing of the device.

[0010] Further, a groove is provided on the side of the housing close to the top plate. A hexagonal groove is provided at the bottom of the inner side surface of the groove, and a number of hexagonal grooves are evenly distributed inside the groove. Tubes are symmetrically arranged outside the housing. The two tubes are centrosymmetrically arranged with the housing, and the sides of the two tubes close to each other are fixedly connected to both sides of the housing respectively. The inner cavity of the tube is communicated with the groove. The arrangement of the groove and the hexagonal groove can reduce the weight of the housing, facilitate the driving of the telescopic rod, and the even distribution of a number of hexagonal grooves forms a honeycomb structure. The honeycomb structure bears pressure evenly, which can improve the strength of the housing, avoid the reduction of the housing strength due to grooving, and the arrangement of the tubes enables the cavity of the housing to be evacuated for heat preservation or liquid to be introduced to adjust the liquid temperature, thereby adjusting the temperature of the environment where the bio-enzyme is located, ensuring that the bio-enzyme is within a suitable temperature range, and avoiding the inactivation of the bio-enzyme during screening.

[0011] Further, the limiting mechanism includes a limiting disk. Circular holes are provided on the surface of the limiting disk, and a number of circular holes are provided corresponding to the outer cylinder. The diameter of the circular hole is slightly larger than that of the outer cylinder. A connecting plate is fixedly connected to the outer side surface of the limiting disk. The side of the connecting plate away from the limiting disk is fixedly connected to the corner of the inner side surface of the housing. A number of connecting plates are provided, and a number of the connecting plates are evenly distributed along the circumference of the limiting disk. The arrangement of a number of circular holes sleeved outside the corresponding number of outer cylinders has a limiting effect.

[0012] Further, a ball is rotatably connected to the inner side surface of the circular hole. The side of the ball away from the inner side surface of the circular hole is in rolling connection with the outer side surface of the outer cylinder. When the outer cylinder is inserted into the limiting disk, the ball rotates, reducing the frictional resistance and facilitating its insertion. When the outer cylinder rotates, the ball rolls on the outer side surface of the outer cylinder, limiting the outer cylinder. At the same time, the rolling frictional resistance is small, which is not easy to affect the rotation speed of the outer cylinder, and the heat generated by friction is also low, and it will not cause the clear liquid to heat up and affect the enzyme activity.

[0013] The beneficial effects of the present invention are: The present invention arranges a centrifugal mechanism, and the gravity of the clear liquid in the outer cylinder overcomes the friction between the driving component and the outer cylinder. The shield mechanism finally drives the inner cylinder to rise to a certain height, and the bottom of the inner cylinder is directly above the clear liquid in the outer cylinder. The outer cylinder and the inner cylinder are continuously driven to rotate. Under the centrifugal effect, the clear liquid remaining in the precipitate is thrown out, and the precipitate is preliminarily dried to avoid the waste of enzymes in the clear liquid. The setting of the limiting mechanism can limit the outer cylinder to avoid the plug-in outer cylinder from being separated from the driving mechanism, and at the same time avoid the wear of the plug-in part of the two. The narrow and long elliptical filter holes at the bottom of the inner cylinder can be opened more densely within a limited surface area to obtain a better filtering effect.

[0014] The present invention arranges a torsion bar. When the outer cylinder and the inner cylinder rotate synchronously, since the torsion bar is arranged in a spiral shape, the spiral shape is unevenly stressed during rotation, causing the inner cylinder to shake irregularly. The bottom of the inner cylinder swings irregularly in the clear liquid inside the outer cylinder, which can swing the sediment away from the inside of the filter hole, thereby preventing the sediment from continuously accumulating inside the filter hole under the action of centrifugal force and preventing the filter hole from being blocked. When the sediment gradually settles into the filter hole at the bottom of the inner cylinder due to the centrifugal force, the sediment is stuck in the filter hole and cannot rotate with the clear liquid, causing the center of gravity of the inner cylinder to drop and be in a non-coincident state with the axis center of the rotating shaft. At this time, the swing of the inner cylinder intensifies, and the sediment is swung away in time to avoid blockage in time.

[0015] The present invention arranges butterfly plates, and the butterfly plates on both sides of the rotating shaft naturally hang down under the action of gravity, which is convenient for adding raw liquid. Under the action of centrifugal force, the two butterfly plates rotate around the rotating shaft and finally form a complete circular plate shape, covering the opening of the inner cylinder to avoid splashing of raw liquid during centrifugation. The inner cylinder rotates 180 degrees along the axial direction of the rotating shaft. When the inner cylinder rotates, the air continuously contacts the inner wall of the filter hole, gradually completely dredges the filter hole, and dredges all the filter holes that may be blocked, avoiding the need for disassembly and cleaning when the device is running. The filter residue accumulates on the slope of the butterfly plate. After shutdown, the butterfly plate cannot fall naturally, and the filter residue will not fall off at will. The butterfly plate is pressed to enable it to rotate, and the filter residue is broken into large pieces. Under the action of gravity, the two butterfly plates rotate into a V shape toward the opening of the inner cylinder, which is convenient for the falling of blocky filter residues, avoiding the filter residues adhering to the inner wall of the inner cylinder and being difficult to clean. At the same time, the falling of large pieces of dry filter residues is not easy to generate dust.

[0016] The present invention provides a shield mechanism, and the opening of the groove body and the hexagonal groove can reduce the weight of the shell, facilitate the driving of the telescopic rod, and the uniform distribution of a plurality of hexagonal grooves forms a honeycomb structure. The honeycomb structure is evenly pressure-bearing, which can improve the strength of the shell and avoid the reduction of the shell strength due to the grooves. The arrangement of the tube body enables the shell cavity to be evacuated for insulation, or liquid to be passed to adjust the liquid temperature, thereby adjusting the temperature of the environment in which the biological enzyme is located, ensuring that the biological enzyme is within a suitable temperature range, and avoiding the inactivation of the biological enzyme during screening.

[0017] By setting a limiting mechanism and arranging a number of round holes to sleeved outside the corresponding number of outer cylinders, a limiting effect is achieved. When the outer cylinder is inserted into the limiting disk, the balls rotate, reducing the frictional resistance and facilitating its insertion. When the outer cylinder rotates, the balls roll on the outer side surface of the outer cylinder to limit the outer cylinder. At the same time, the rolling frictional resistance is small, which is not easy to affect the rotation speed of the outer cylinder, and the heat generated by friction is also low, so it will not cause the temperature rise of the clarified liquid and affect the enzyme activity. Brief Description of the Drawings

[0018] Figure 1 It is a schematic diagram of the stock solution screening and filtering device for the preparation of the biological enzyme preparation of the present invention; Figure 2 It is a schematic diagram of the structure of the centrifugal mechanism of the present invention; Figure 3 It is a schematic diagram of the internal structure of the outer cylinder of the present invention; Figure 4 It is a schematic diagram of the structure of the filter holes of the present invention; Figure 5 It is a schematic diagram of the sectional structure of the inner cylinder of the present invention; Figure 6 It is a schematic diagram of the structure of the driving component of the present invention; Figure 7 It is a schematic diagram of the structure of the driving mechanism of the present invention; Figure 8 It is a schematic diagram of the structure of the shield mechanism of the present invention; Figure 9 It is a schematic diagram of the sectional structure of the limiting disk of the present invention; Figure 10 For the present invention Figure 9 Enlarged view of part A.

[0019] In the figure: 1. bottom plate; 2. driving mechanism; 21. driving box; 22. driving disk; 23. driving rod; 24. chuck; 3. top plate; 4. shield mechanism; 41. outer shell; 42. groove body; 43. hexagonal groove; 44. pipe body; 45. telescopic rod; 5. centrifugal mechanism; 51. outer cylinder; 52. inner cylinder; 53. rotating shaft; 54. torsion bar; 55. driving component; 551. ring body; 552. sliding plate; 553. motor; 554. rotating shaft; 555. sealing plate; 56. butterfly plate; 57. buffer rod; 58. hemisphere; 59. filter hole; 6. limiting mechanism; 61. limiting disk; 62. connecting plate; 63. round hole; 64. ball. Detailed Embodiments

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are given for the purpose of illustration and description, and are not exhaustive or limited to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thus design various embodiments with various modifications suitable for specific purposes.

[0021] Example 1. Refer to Figures 1 - 8 , the present invention is a stock solution screening and filtering device for preparing biological enzyme preparations, including: A bottom plate 1, on the top of which a driving mechanism 2 is fixedly connected. Above the driving mechanism 2, a centrifugal mechanism 5 is inserted. The driving mechanism 2 is used to drive the centrifugal mechanism 5 to rotate and centrifuge. A shield mechanism 4, which is fixedly connected to the top of the bottom plate 1, and the inner side surface of the shield mechanism 4 is slidably connected to the outer side surface of the driving mechanism 2. The top of the shield mechanism 4 is fixedly connected to a top plate 3. A limiting mechanism 6, which is fixedly connected to the inner side surface of the shield mechanism 4, and the inner side surface of the limiting mechanism 6 is slidably connected to the outer side surface of the centrifugal mechanism 5. The limiting mechanism 6 is used to limit the centrifugal rotation of the centrifugal mechanism 5. Among them, the centrifugal mechanism 5 includes: An outer cylinder 51, several outer cylinders 51 are arranged directly above the driving mechanism 2, and an inner cylinder 52 is arranged inside each of the several outer cylinders 51. A filter hole 59 is opened at the bottom of the inner cylinder 52, and the filter hole 59 is set as a long and narrow ellipse. There are several filter holes 59, and the several filter holes 59 are evenly distributed at the bottom of the inner cylinder 52. A rotating shaft 53, which is arranged inside the inner cylinder 52. The two ends of the rotating shaft 53 penetrate through the inner cylinder 52, and the surface of the rotating shaft 53 is fixedly connected to the inner side surface of the inner cylinder 52. Torsion bars 54, which are symmetrically arranged at the interval between the outer cylinder 51 and the inner cylinder 52. The closer ends of the two torsion bars 54 are respectively fixedly connected to the two ends of the rotating shaft 53. The torsion bars 54 are set as spiral shapes. A driving component 55, the surface of which is slidably and limit-connected to the inner side surface of the outer cylinder 51. The farther ends of the two torsion bars 54 are both fixedly connected to the surface of the driving component 55. The output end of the driving component 55 drives the two torsion bars 54 to rotate synchronously. The side of the driving component 55 away from the outer cylinder 51 is rotationally and limit-connected to the side of the top plate 3 close to the bottom plate 1. The shield mechanism 4 is started, and the shield mechanism 4 drives the top plate 3 to rise, driving the limit mechanism 6, the driving assembly 55, and the inner cylinder 52 to rise. The outer cylinder 51, which is slidingly connected to the driving assembly 55, also rises under the action of friction. Then, the outer cylinder 51 is partially pulled out, and the top of the inner cylinder 52 leaks out. The original liquid is added to the inner cylinder 52, and the outer cylinder 51 is reset. Then, the shield mechanism 4 is started again, the device is reset, and the outer cylinder 51 is re-inserted into the output end of the driving mechanism 2. The driving mechanism 2 is started, and the driving mechanism 2 drives the outer cylinder 51, the driving assembly 55, and the inner cylinder The inner cylinder 52 rotates to centrifuge the stock solution to precipitate impurities, bacteria, unreacted substrates, etc. in the stock solution. At this time, the inner cylinder 52 is inside the clarified stock solution in the outer cylinder 51, and the precipitates are trapped in the inner cylinder 52 by the filter holes 59. Then the shield mechanism 4 is started, and the gravity of the clear liquid in the outer cylinder 51 overcomes the friction between the driving assembly 55 and the outer cylinder 51. The shield mechanism 4 finally drives the inner cylinder 52 to rise to a certain height, and the bottom of the inner cylinder 52 is just above the clear liquid in the outer cylinder 51. The outer cylinder 51 and the inner cylinder 52 are driven to rotate continuously. Under the action of centrifugation, the precipitates are The clear liquid remaining in the sediment is thrown out to make the sediment initially dry, avoiding the waste of enzymes in the clear liquid. The setting of the limiting mechanism 6 can limit the outer cylinder 51 to avoid the plugged outer cylinder 51 from being separated from the driving mechanism 2, and at the same time avoid the wear of the plug-in part of the two. The narrow and long elliptical filter holes 59 can be opened more densely within a limited surface area to obtain a better filtering effect. When the outer cylinder 51 and the inner cylinder 52 rotate synchronously, since the torsion bar 54 is set in a spiral shape, the spiral is unevenly stressed during rotation, which will cause the inner cylinder 52 to shake irregularly. The bottom of the inner cylinder 52 swings irregularly in the clear liquid inside the outer cylinder 51, which can swing the sediment from the inside of the filter hole 59, avoiding the continuous accumulation of sediment inside the filter hole 59 under the action of centrifugal force, and avoiding the blockage of the filter hole 59. When the sediment gradually settles into the filter hole 59 at the bottom of the inner cylinder 52 due to the centrifugal force, the sediment is stuck in the filter hole 59 and cannot rotate with the clear liquid, causing the center of gravity of the inner cylinder 52 to drop and be in a non-coincident state with the axis of the rotating shaft 53. At this time, the swing of the inner cylinder 52 intensifies, and the sediment is swung away in time to avoid blockage in time.

[0022] Inside the inner cylinder 52, there are two butterfly plates 56. The two butterfly plates 56 are rotatably connected on the side close to each other. The rotating shaft 53 penetrates through the connection of the two butterfly plates 56, and the surface of the rotating shaft 53 is rotatably connected to the inner side surface of the butterfly plate 56. The side of the butterfly plate 56 away from the rotating shaft 53 is serrated. The butterfly plates 56 on both sides of the rotating shaft 53 naturally droop under the action of gravity, which is convenient for the addition of the stock solution. The outermost circle of the butterfly plate 56 close to the filter holes 59 has a slope. During centrifugation, the butterfly plate 56 rotates with the rotation of the rotating shaft 53. Under the action of centrifugal force, the two butterfly plates 56 rotate around the rotating shaft 53 and finally form a complete circular plate to cover the opening of the inner cylinder 52 to prevent the stock solution from splashing during centrifugation. Then, the driving assembly 55 is started. The driving assembly 55 drives the torsion bar 54 and the rotating shaft 53 to rotate, and finally drives the inner cylinder 52 to rotate 180 degrees along the axial direction of the rotating shaft 53. Then, the inner cylinder 52 is driven to rotate along its axial direction again, and the two butterfly plates 56 flatten. When the inner cylinder 52 rotates, the air continuously contacts the inner wall of the filter holes 59 and gradually completely dredges the filter holes 59. The sediment drops onto the surface of the butterfly plate 56 and is caught at the slope of the butterfly plate 56 and the inner wall of the inner cylinder 52 under the action of centrifugal force, and the remaining clear liquid is squeezed out from the gap between the serrations of the butterfly plate 56 and the inner cylinder 52, thoroughly screening the clear liquid. Moreover, when the inner cylinder 52 rotates, the air continuously contacts the inner wall of the filter holes 59 and gradually completely dredges all the filter holes 59 that may be blocked, avoiding the need for disassembly and cleaning during the operation of the device. And the filter residue accumulates at the slope of the butterfly plate 56. After the machine stops, the butterfly plate 56 cannot naturally drop, and the filter residue will not fall randomly. After preparing a container for holding the filter residue below the butterfly plate 56, press the butterfly plate 56 to make it rotate. At the same time, the filter residue breaks into large pieces. The two butterfly plates 56 rotate into a V shape towards the opening of the inner cylinder 52 under the action of gravity, which is convenient for the dropping of the large pieces of filter residue. While avoiding the filter residue from adhering to the inner wall of the inner cylinder 52 and being difficult to clean, the dropping of the large and dry filter residue is not likely to generate dust.

[0023] The outer side surface of the inner cylinder 52 is fixedly connected with buffer rods 57. A number of buffer rods 57 are evenly distributed along the circumferential direction of the inner cylinder 52. The buffer rods 57 are spiral-shaped. The side of the buffer rod 57 away from the inner cylinder 52 is fixedly connected with hemispheres 58. The side of the hemisphere 58 away from the buffer rod 57 is slidably connected with the inner side surface of the outer cylinder 51. When the inner cylinder 52 shakes, it drives the buffer rod 57 to move, drives the hemisphere 58 to squeeze the inner cylinder 52, and the hemisphere 58 slides on the inner wall of the outer cylinder 51, and the buffer rod 57 bends. Then, under the action of its bending elasticity, the force of the inner cylinder 52's swing is consumed, avoiding the outer cylinder 51 from swinging, and at the same time restricting the swing amplitude of the inner cylinder 52.

[0024] The driving component 55 includes an annular body 551. The annular body 551 is arranged directly above the outer cylinder 51. One side of the annular body 551 away from the outer cylinder 51 is inlaid on one side of the top plate 3 close to the bottom plate 1, and the surface of the annular body 551 is in limit rotational connection with the inner side surface of the top plate 3. A sliding plate 552 is fixedly connected to the side of the annular body 551 away from the top plate 3. The sliding plates 552 are symmetrically arranged on the side of the annular body 551 away from the top plate 3. The sides of the two sliding plates 552 away from the annular body 551 both extend into the inner part of the outer cylinder 51. The two sides of the sliding plate 552 are in the shape of a dovetail, and the surface of the sliding plate 552 is in sliding connection with the outer cylinder 51. The sliding plate 552 and the outer cylinder 51 which are in limit sliding connection through the dovetail structure can be disassembled while the outer cylinder 51 is being removed without affecting the rotation of the sliding plate 552 along with the outer cylinder 51. When the outer cylinder 51 is empty, the frictional force between the outer cylinder 51 and the sliding plate 552 is greater than the self-weight of the outer cylinder 51, and the outer cylinder 51 is taken out together with the sliding plate 552. When there is clear liquid in the outer cylinder 51, the frictional force between the outer cylinder 51 and the sliding plate 552 is less than the sum of the self-weight of the outer cylinder 51 and the clear liquid, and the outer cylinder 51 remains on the top of the driving mechanism 2 for convenient removal of the clear liquid.

[0025] Motors 553 are inlaid in the interiors of the two sliding plates 552 respectively. The outer side surfaces of the motors 553 are fixedly connected to the inner side surfaces of the sliding plates 552. Rotating shafts 554 are fixedly connected to the sides of the two motors 553 close to each other. The ends of the two rotating shafts 554 close to each other are respectively fixedly connected to the ends of the two torsion bars 54 away from each other. A sealing plate 555 is fixedly connected to the side of the motor 553 close to the rotating shaft 554. The inner side surface of the sealing plate 555 is in rotational connection with the surface of the rotating shaft 554, and the diameter of the sealing plate 555 is greater than the diameter of the motor 553. When the inner cylinder 52 needs to be flipped, the motors 553 are started. The output ends of the motors 553 drive the rotating shafts 554 to rotate, driving the torsion bars 54 to rotate. Finally, the two motors 553 drive the two ends of the rotating shaft 53 to rotate synchronously, driving the inner cylinder 52 to flip. The setting of the sealing plate 555 can prevent the clear liquid from contacting the motors 553, achieving the effect of protecting the motors 553.

[0026] The driving mechanism 2 includes a driving box 21. The driving box 21 is fixedly connected to the top of the bottom plate 1. On one side of the driving box 21 away from the bottom plate 1, there is a driving disc 22. The surface of the driving disc 22 is fixedly connected to the inner side surface of the driving box 21. A driving rod 23 is rotatably connected to the inner side surface of the driving disc 22, and the driving rod 23 is driven to rotate by the driving box 21. There are several driving rods 23 corresponding to the outer cylinder 51. The side of the driving rod 23 away from the driving disc 22 is in a dovetail shape. A chuck 24 is arranged on the side of the driving rod 23 away from the driving disc 22. The inner side surface of the chuck 24 is inserted into the side of the driving rod 23 away from the driving disc 22. The side of the chuck 24 away from the driving disc 22 is fixedly connected to the side of the outer cylinder 51 close to the driving disc 22. The driving box 21 drives the driving rod 23 to rotate, the driving rod 23 drives the chuck 24 to rotate, and finally drives the outer cylinder 51 to rotate. The inserted chuck 24 and the driving rod 23 can facilitate the removal of the outer cylinder 51.

[0027] The protective cover mechanism 4 includes a housing 41. The inner side surface of the housing 41 is slidably connected to the outer side surface of the driving box 21. The side of the housing 41 away from the bottom plate 1 is fixedly connected to the side of the top plate 3 close to the bottom plate 1. A telescopic rod 45 is fixedly connected to the side of the housing 41 close to the bottom plate 1. The side of the telescopic rod 45 away from the housing 41 is fixedly connected to the top of the bottom plate 1. When the telescopic rod 45 is started, the telescopic rod 45 expands and contracts, driving the housing 41 and the top plate 3 to rise and fall to complete the opening and closing of the device.

[0028] A groove 42 is opened on the side of the housing 41 close to the top plate 3. A hexagonal groove 43 is opened at the bottom of the inner side surface of the groove 42, and several hexagonal grooves 43 are evenly distributed inside the groove 42. The outer side of the housing 41 is symmetrically provided with pipe bodies 44. The two pipe bodies 44 are centrosymmetrically arranged with the housing 41, and the sides of the two pipe bodies 44 close to each other are respectively fixedly connected to the two sides of the housing 41. The inner cavity of the pipe body 44 communicates with the groove 42. The opening of the groove 42 and the hexagonal groove 43 can reduce the weight of the housing 41, facilitate the driving of the telescopic rod 45, and the uniform distribution of several hexagonal grooves 43 forms a honeycomb structure. The honeycomb structure bears pressure evenly, which can improve the strength of the housing 41 and prevent the strength of the housing 41 from decreasing due to grooving. The setting of the pipe body 44 enables the cavity of the housing 41 to be evacuated for heat preservation, or liquid can be introduced to adjust the liquid temperature, thereby adjusting the temperature of the environment where the biological enzyme is located, ensuring that the biological enzyme is within a suitable temperature range and preventing the biological enzyme from inactivating during sieving.

[0029] Example 2, please refer to Figures 1 - 10, the limiting mechanism 6 includes a limiting disk 61. A circular hole 63 is formed on the surface of the limiting disk 61, and a number of circular holes 63 are provided corresponding to the outer cylinder 51. The diameter of the circular hole 63 is slightly larger than that of the outer cylinder 51. A connecting plate 62 is fixedly connected to the outer side surface of the limiting disk 61. The side of the connecting plate 62 away from the limiting disk 61 is fixedly connected to the corner of the inner side surface of the housing 41. A number of connecting plates 62 are provided, and the number of connecting plates 62 is evenly distributed along the circumference of the limiting disk 61. The arrangement of a number of circular holes 63 sleeved on the corresponding number of outer cylinders 51 plays a limiting effect.

[0030] A ball 64 is rotatably connected to the inner side surface of the circular hole 63. The side of the ball 64 away from the inner side surface of the circular hole 63 is in rolling connection with the outer side surface of the outer cylinder 51. When the outer cylinder 51 is inserted into the limiting disk 61, the ball 64 rotates to reduce the frictional resistance and facilitate its insertion. When the outer cylinder 51 rotates, the ball 64 rolls on the outer side surface of the outer cylinder 51 to limit the outer cylinder 51. At the same time, the rolling frictional resistance is small, which is not easy to affect the rotation speed of the outer cylinder 51, and the heat generated by friction is also low, and it will not cause the clear liquid to heat up and affect the enzyme activity.

[0031] During use, extend the telescopic rod 45. The telescopic rod 45 extends and retracts, driving the outer shell 41 and the top plate 3 to rise, driving the top plate 3 to rise, and driving the limit mechanism 6, the driving assembly 55, and the inner cylinder 52 to rise. When the outer cylinder 51 is empty, the friction between the outer cylinder 51 and the sliding plate 552 is greater than the self-weight of the outer cylinder 51. The outer cylinder 51, which is in limited sliding connection with the driving assembly 55, also rises under the action of the friction force. Then, pull out part of the outer cylinder 51, and the top of the inner cylinder 52 is exposed. Add the stock solution into the inner cylinder 52, reset the outer cylinder 51, and then start the telescopic rod 45 again to drive the top plate 3 to reset. The outer cylinder 51 is inserted into the limit disc 61, and the ball 64 rolls on the surface of the outer cylinder 51. The chuck 24 at the bottom of the outer cylinder 51 is clamped with the driving rod 23. The driving box 21 drives the driving rod 23 to rotate. The driving rod 23 drives the chuck 24 to rotate, driving the outer cylinder 51 to rotate, driving the sliding plate 552, the motor 553, the rotating shaft 554, the torsion bar 54, the rotating shaft 53, and the inner cylinder 52 to rotate. The butterfly plate 56 rotates as the rotating shaft 53 rotates. Under the action of the centrifugal force, the two butterfly plates 56 rotate around the rotating shaft 53 and finally form a complete circular plate shape to cover the opening of the inner cylinder 52, centrifuge the stock solution, and precipitate impurities, bacteria, unreacted substrates, etc. in the stock solution. At this time, the inner cylinder 52 is inside the clarified stock solution in the outer cylinder 51, and the precipitate is intercepted inside the inner cylinder 52 by the filter holes 59. After the impurities are completely precipitated, start the telescopic rod 45. The telescopic rod 45 drives the outer shell 41 and the top plate 3 to rise, and then drives the ring body 551, the sliding plate 552, the motor 553, the rotating shaft 554, the torsion bar 54, the rotating shaft 53, and the inner cylinder 52 to rise. The bottom of the inner cylinder 52 is directly above the clear liquid inside the outer cylinder 51. Continue to drive the outer cylinder 51 and the inner cylinder 52 to rotate. Under the centrifugal action, the remaining clear liquid in the precipitate is thrown out to preliminarily dry the precipitate. Start the motor 553. The output end of the motor 553 drives the rotating shaft 554 to rotate, driving the torsion bar 54 to rotate. Finally, the two motors 553 drive the two ends of the rotating shaft 53 to rotate synchronously, driving the inner cylinder 52 to turn over. Then, drive the inner cylinder 52 and the outer cylinder 51 to rotate centrifugally again. Under the action of the centrifugal force, the two butterfly plates 56 flatten. When the inner cylinder 52 rotates, the air continuously contacts the inner wall of the filter holes 59 and gradually completely dredges the filter holes 59. The precipitate falls onto the surface of the butterfly plate 56 and is caught at the slope of the butterfly plate 56 and the inner wall of the inner cylinder 52 under the action of the centrifugal force. The remaining clear liquid is squeezed out from the gap between the serrated shape of the butterfly plate 56 and the inner cylinder 52 to completely screen the clear liquid. And when the inner cylinder 52 rotates, the air continuously contacts the inner wall of the filter holes 59 and gradually completely dredges the filter holes 59. The filter residue accumulates at the slope of the butterfly plate 56. After shutdown, the butterfly plate 56 cannot naturally descend, and the filter residue will not fall randomly. After preparing a container for holding the filter residue below the butterfly plate 56, press the butterfly plate 56 to make it rotate. At the same time, the filter residue breaks into large pieces. The two butterfly plates 56 rotate into a V shape towards the opening of the inner cylinder 52 under the action of gravity, and the blocky filter residue falls off to complete the screening.

[0032] Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art and related fields based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention. Structures, devices, and operation methods not specifically described and explained in the present invention shall be implemented by conventional means in the art unless otherwise specified and limited.

Claims

1. A stock solution screening and filtering device for the preparation of a biological enzyme preparation, characterized in that, Including: A bottom plate (1), on the top of which a driving mechanism (2) is fixedly connected, and a centrifugal mechanism (5) is inserted directly above the driving mechanism (2); A shield mechanism (4), which is fixedly connected to the top of the bottom plate (1), and the inner side surface of the shield mechanism (4) is slidably connected to the outer side surface of the driving mechanism (2), and the top of the shield mechanism (4) is fixedly connected to a top plate (3); A limiting mechanism (6), which is fixedly connected to the inner side surface of the shield mechanism (4), and the inner side surface of the limiting mechanism (6) is slidably connected to the outer side surface of the centrifugal mechanism (5); Wherein, the centrifugal mechanism (5) includes: An outer cylinder (51), several of which are arranged directly above the driving mechanism (2), and an inner cylinder (52) is arranged inside each of the several outer cylinders (51). A filter hole (59) is formed at the bottom of the inner cylinder (52), and the filter hole (59) is set as a long and narrow ellipse. There are several filter holes (59), and the several filter holes (59) are evenly distributed at the bottom of the inner cylinder (52); A rotating shaft (53), which is arranged inside the inner cylinder (52), and both ends of the rotating shaft (53) penetrate through the inner cylinder (52), and the surface of the rotating shaft (53) is fixedly connected to the inner side surface of the inner cylinder (52); Torsion bars (54), which are symmetrically arranged at the interval between the outer cylinder (51) and the inner cylinder (52), and the mutually approaching ends of the two torsion bars (54) are respectively fixedly connected to both ends of the rotating shaft (53), and the torsion bars (54) are set as spiral shapes; A driving assembly (55), the surface of which is limited and slidably connected to the inner side surface of the outer cylinder (51), and the mutually remote ends of the two torsion bars (54) are both fixedly connected to the surface of the driving assembly (55), and the side of the driving assembly (55) away from the outer cylinder (51) is rotationally limited and connected to the side of the top plate (3) close to the bottom plate (1).

2. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 1, characterized in that: A butterfly plate (56) is arranged inside the inner cylinder (52). There are two butterfly plates (56), and the mutually approaching sides of the two butterfly plates (56) are rotatably connected. The rotating shaft (53) penetrates through the connection part of the two butterfly plates (56), and the surface of the rotating shaft (53) is rotatably connected to the inner side surface of the butterfly plate (56). The side of the butterfly plate (56) away from the rotating shaft (53) is set as serrated, and the outermost circle of the side of the butterfly plate (56) close to the filter hole (59) has a slope.

3. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 2, characterized in that: A buffer rod (57) is fixedly connected to the outer side surface of the inner cylinder (52). There are several buffer rods (57) evenly distributed along the circumferential direction of the inner cylinder (52). The buffer rods (57) are set as spiral shapes. The side of the buffer rod (57) away from the inner cylinder (52) is fixedly connected to a hemisphere (58), and the side of the hemisphere (58) away from the buffer rod (57) is slidably connected to the inner side surface of the outer cylinder (51).

4. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 3, characterized in that: The driving component (55) includes an annular body (551). The annular body (551) is arranged directly above the outer cylinder (51). One side of the annular body (551) away from the outer cylinder (51) is inlaid on one side of the top plate (3) close to the bottom plate (1), and the surface of the annular body (551) is in limit rotational connection with the inner side surface of the top plate (3). A sliding plate (552) is fixedly connected to one side of the annular body (551) away from the top plate (3). The sliding plates (552) are symmetrically arranged on one side of the annular body (551) away from the top plate (3). One side of each of the two sliding plates (552) away from the annular body (551) extends into the inner part of the outer cylinder (51). The two sides of the sliding plate (552) are in the shape of a dovetail, and the surface of the sliding plate (552) is in sliding connection with the outer cylinder (51).

5. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 4, characterized in that: Motors (553) are inlaid in the interiors of the two sliding plates (552) respectively. The outer side surface of each motor (553) is fixedly connected to the inner side surface of the corresponding sliding plate (552). A rotating shaft (554) is fixedly connected to one side of each of the two motors (553) close to each other. One end of each of the two rotating shafts (554) close to each other is fixedly connected to one end of each of the two torsion bars (54) away from each other. A sealing plate (555) is fixedly connected to one side of the motor (553) close to the rotating shaft (554). The inner side surface of the sealing plate (555) is in rotational connection with the surface of the rotating shaft (554), and the diameter of the sealing plate (555) is larger than the diameter of the motor (553).

6. The original liquid screening and filtering device for preparing a biological enzyme preparation according to claim 5, characterized in that: The driving mechanism (2) includes a driving box (21). The driving box (21) is fixedly connected to the top of the bottom plate (1). A driving disk (22) is arranged on one side of the driving box (21) away from the bottom plate (1). The surface of the driving disk (22) is fixedly connected to the inner side surface of the driving box (21). A driving rod (23) is rotatably connected to the inner side surface of the driving disk (22), and the driving rod (23) is driven to rotate by the driving box (21). A number of driving rods (23) are arranged corresponding to the outer cylinder (51). One side of the driving rod (23) away from the driving disk (22) is in the shape of a dovetail. A chuck (24) is arranged on one side of the driving rod (23) away from the driving disk (22), and the inner side surface of the chuck (24) is inserted into one side of the driving rod (23) away from the driving disk (22). One side of the chuck (24) away from the driving disk (22) is fixedly connected to one side of the outer cylinder (51) close to the driving disk (22).

7. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 6, characterized in that: The shield mechanism (4) includes a housing (41). The inner side surface of the housing (41) is in sliding connection with the outer side surface of the driving box (21). One side of the housing (41) away from the bottom plate (1) is fixedly connected to one side of the top plate (3) close to the bottom plate (1). A telescopic rod (45) is fixedly connected to one side of the housing (41) close to the bottom plate (1). One side of the telescopic rod (45) away from the housing (41) is fixedly connected to the top of the bottom plate (1).

8. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 7, characterized in that: A groove (42) is formed on one side of the outer shell (41) close to the top plate (3). A hexagonal groove (43) is formed at the bottom of the inner side surface of the groove (42), and a plurality of the hexagonal grooves (43) are evenly distributed inside the groove (42). Pipe bodies (44) are symmetrically arranged outside the outer shell (41). The two pipe bodies (44) are centrosymmetrically arranged with respect to the outer shell (41), and the two sides of the two pipe bodies (44) close to each other are fixedly connected to both sides of the outer shell (41) respectively. The inner cavity of the pipe body (44) is communicated with the groove (42).

9. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 8, wherein: The limiting mechanism (6) includes a limiting disk (61). A circular hole (63) is formed on the surface of the limiting disk (61), and a plurality of the circular holes (63) are arranged corresponding to the outer cylinder (51). The diameter of the circular hole (63) is slightly larger than that of the outer cylinder (51). A connecting plate (62) is fixedly connected to the outer side surface of the limiting disk (61). The side of the connecting plate (62) away from the limiting disk (61) is fixedly connected to the corner of the inner side surface of the outer shell (41). A plurality of the connecting plates (62) are provided, and the plurality of the connecting plates (62) are evenly distributed along the circumferential direction of the limiting disk (61).

10. The stock solution screening and filtering device for preparing a biological enzyme preparation according to claim 9, characterized in that: A ball (64) is rotatably connected to the inner side surface of the circular hole (63), and the side of the ball (64) away from the inner side surface of the circular hole (63) is in rolling connection with the outer side surface of the outer cylinder (51).

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