Microfluidic detection device and method of use thereof

Abstract

The application discloses a micro-fluidic detection device and a use method thereof, relates to the technical field of micro-fluidic detection, and comprises a detection device, a movable plate movably connected to the side of the detection device, a micro-fluidic chip arranged on the inner side of the movable plate, a movable frame rotatably connected to the upper portion of the detection device, an adjusting mechanism arranged on the movable frame, the adjusting mechanism comprising a first servo motor and a second servo motor mounted on the movable frame and an L-shaped plate movably connected to the outer side of the movable frame, an open groove being formed in the outer side of the L-shaped plate, and a control system being arranged on the movable frame. The control system can be used for real-time identification of the injection port position of the micro-fluidic chip, then the adjusting mechanism is controlled to drive the L-shaped plate to move, so that the L-shaped plate is aligned with the injection port required by a user, the liquid gun can be more accurately aligned with the injection port of the micro-fluidic chip, and problems such as slow alignment by manual operation, liquid splashing, and position deviation of liquid dripping are avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microfluidic detection, and particularly to a microfluidic detection device and a use method thereof. BACKGROUND

[0002] The microfluidic detection device is a detection tool based on microfluidic technology, which uses small channels or chambers to manipulate and measure fluid flow, and is usually used for precise fluid control and measurement in the space of microns to nanometers. The microfluidic detection device can realize efficient, sensitive and accurate detection and analysis of biomolecules, and uses the characteristic that the fluorescently labeled biomolecules emit fluorescence under excitation light irradiation for detection, and then introduces the sample to be tested into the microfluidic chip by designing a specific microfluidic structure, and combines with the fluorescently labeled probe molecules. Under excitation light irradiation, the change of the fluorescence signal is observed, so as to realize qualitative and quantitative analysis of biomolecules.

[0003] When the user introduces the sample to be tested into the injection port of the microfluidic chip by using a pipette, the user needs to observe the position of the injection port with the eyes, and then manually aims the nozzle of the pipette at the top of the injection port. Since the injection port is small, the alignment process is time-consuming, and liquid splashing and droplet position deviation caused by unstable operation are prone to occur. Therefore, a microfluidic detection device and a use method thereof are provided. SUMMARY

[0004] The purpose of the present application is to solve the problem in the prior art that the user needs to observe the position of the injection port with the eyes, and then manually aims the nozzle of the pipette at the top of the injection port. Since the injection port is small, the alignment process is time-consuming, and liquid splashing and droplet position deviation caused by unstable operation are prone to occur. Therefore, a microfluidic detection device and a use method thereof are provided.

[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme:

[0006] A microfluidic detection device, comprising a detection device, a movable plate movably connected to the side of the detection device, a microfluidic chip arranged on the inner side of the movable plate, an activity frame rotatably connected to the upper part of the detection device, an adjusting mechanism arranged on the activity frame, the adjusting mechanism comprising a first servo motor and a second servo motor mounted on the activity frame, and an L-shaped plate movably connected to the outer side of the activity frame, an opening groove being formed on the outer side of the L-shaped plate, a control system arranged on the activity frame, the control system comprising:

[0007] An image acquisition module for capturing images of the microfluidic chip and its injection port and transmitting them to an image processing module;

[0008] An image processing module is configured to process images collected by the image collection module, extract feature information of the injection port, and identify the positions and features of all injection ports on the microfluidic chip.

[0009] An image recognition module is configured to match the feature information extracted by the image processing module with a preset injection port feature library through a feature matching algorithm, and determine the identity of each injection port.

[0010] A position feedback and adjustment module is configured to feed back the position information of the selected injection port to the first and second servo motors, and control the first and second servo motors to adjust the position of the L-shaped plate through a path planning algorithm.

[0011] A human-computer interaction module is configured to provide a user operation interface and allow the user to select a target injection port.

[0012] After the microfluidic chip is placed in the movable plate, the image collection module captures images of the microfluidic chip and its injection ports, and transmits the images to the image processing module. The image processing module pre-processes the images and detects targets, identifies the positions and features of all injection ports, and transmits the feature information to the image recognition module. The image recognition module confirms the identity of the injection ports identified by the image processing module, and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module. The position feedback and adjustment module feeds back the user's selection to the first and second servo motors. The first and second servo motors are started to drive the L-shaped plate to move in the horizontal position until the opening slot corresponds to the position of the injection port. Then, the pipette is inserted into the opening slot for guidance and the liquid is dropped into the microfluidic chip. This can ensure that the pipette is more accurately aligned with the injection port of the microfluidic chip, avoid slow positioning caused by manual operation, and help reduce problems such as liquid splashing and drop position deviation caused by unstable operation.

[0013] The above technical solution further comprises:

[0014] The inner side of the movable frame is rotationally connected with a first threaded rod and a guide rod, respectively. The first threaded rod is installed at the output shaft end of the first servo motor. The outer side of the guide rod is slidably provided with a movable rod. The movable rod is threadedly connected between the first threaded rod. When the first threaded rod rotates, it drives the movable rod to move along the guide rod.

[0015] The bottom of the movable rod is provided with a square slot and a sliding slot, respectively. The second servo motor is installed at the inner side of the square slot. The inner side of the sliding slot is slidably provided with a sliding block. The sliding block moves along the sliding slot.

[0016] The second servo motor extends to one end inside the sliding groove, and a second threaded rod is installed at the end, the second threaded rod is in threaded connection with a sliding block, the sliding block is fixedly connected with the L-shaped plate, and rotation of the second threaded rod can drive the sliding block to move along the sliding groove.

[0017] The size of the sliding groove opening is matched with the size of the sliding block, and the cross section of the sliding groove and the sliding block is convex, so as to ensure the stability of the sliding block.

[0018] The image acquisition module comprises an image capturing unit and a data transmission unit, the image capturing unit captures the image of the microfluidic chip and its injection port through a camera and converts it into a digital signal, and the data transmission unit transmits the captured image data to the image processing module in real time.

[0019] The image processing module comprises an image preprocessing unit, a feature extraction unit and a target detection unit, the image preprocessing unit performs denoising and enhancement preprocessing operation on the captured image, the feature extraction unit extracts the feature information of the injection port from the preprocessed image, and the target detection unit identifies the position and characteristics of all injection ports on the microfluidic chip.

[0020] The image recognition module comprises a feature matching unit and a position accuracy correction unit, the feature matching unit matches the extracted feature information with the preset injection port feature library through a feature matching algorithm to determine the identity of each injection port, and the position accuracy correction unit corrects the position of the matched injection port to improve the positioning accuracy.

[0021] The feature matching algorithm is Euclidean distance, and Euclidean distance is used to measure the similarity between two feature points, and the smaller the Euclidean distance, the more similar the two feature points are.

[0022] The calculation formula of Euclidean distance is:

[0023]

[0024] Wherein, f1 and f2 are descriptors of two feature points, f 1i and f 2i is the i-th feature value, and n is the dimension of the feature vector.

[0025] The position feedback and adjustment module comprises a path planning unit, a control unit and a position monitoring unit, the path planning unit calculates the moving path of the L-shaped plate according to the position information of the target injection port through a path planning algorithm, the control unit controls the operation of the first servo motor and the second servo motor, and the position monitoring unit monitors the actual position of the L-shaped plate in real time, the path planning algorithm is A* algorithm, which is used to find the shortest path from the starting point to the target point in the graphic environment, and the algorithm formula is:

[0026] Total path cost F(n): F(n) = G(n) + H(n);

[0027] G(n): actual cost from the starting point to the current node n;

[0028] H(n): estimated cost from the current node n to the target point.

[0029] A method for using a microfluidic detection device, using a microfluidic detection device, comprising the following steps:

[0030] Step one: first pull the movable plate, move the movable plate to the outside of the detection device, then put the microfluidic chip into the groove of the movable plate, then rotate the movable frame until the movable frame is parallel to the movable plate;

[0031] Step two: capture the image of the microfluidic chip and its injection port by the image acquisition module, then the image processing module identifies the position and characteristics of all injection ports, and then the image recognition module determines the accurate position of the target injection port, the user selects the target injection port according to the demand through the man-machine interaction module, and the position feedback and adjustment module feeds back the injection port position information selected by the user to the first servo motor and the second servo motor;

[0032] Step three: the first servo motor and the second servo motor start after receiving the signal, drive the L-shaped plate to move in the horizontal position, until the opening slot corresponds to the position of the injection port, then the staff inserts the pipette into the opening slot for guidance, and drops the liquid into the microfluidic chip, closes the movable plate after the liquid drops, and detects through the detection device.

[0033] The present application has the following beneficial effects:

[0034] 1、In the present application, the injection port position of the microfluidic chip can be identified in real time by setting the control system, then the adjustment mechanism drives the L-shaped plate to move, so that the L-shaped plate is aligned with the injection port position required by the user, which can ensure that the pipette is more accurately aligned with the injection port of the microfluidic chip, and avoids slow alignment when manually operating, and also helps to reduce problems such as liquid splashing, drop position deviation caused by unstable operation. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 It is a whole structure schematic view of a microfluidic detection device and its using method proposed in the present application;

[0036] Figure 2 It is a movable frame structure schematic view in the present application;

[0037] Figure 3 It is a control system schematic view in the present application;

[0038] Figure 4 For Figure 1 structure enlarged schematic view at A in the middle;

[0039] Figure 5 For Figure 2 structure enlarged schematic view at B in the middle;

[0040] Figure 6 For Figure 2 structure enlarged schematic view at C in the middle.

[0041] In the figure: 1, detection device; 2, movable plate; 3, microfluidic chip; 4, movable frame; 5, first servo motor; 6, first threaded rod; 7, guide rod; 8, movable rod; 9, second servo motor; 10, sliding groove; 11, second threaded rod; 12, sliding block; 13, L-shaped plate; 14, open slot; 15, square slot; 16, image acquisition module; 17, image processing module; 18, image recognition module; 19, position feedback and adjustment module; 20, human-computer interaction module. DETAILED DESCRIPTION

[0042] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0043] As Figure 1 - Figure 6 shown, the microfluidic detection device provided by the present application includes a detection device 1, the side of the detection device 1 is movably connected with a movable plate 2, the inner side of the movable plate 2 is provided with a microfluidic chip 3, the upper part of the detection device 1 is rotatably connected with a movable frame 4, the movable frame 4 is provided with an adjustment mechanism, the adjustment mechanism includes a first servo motor 5 and a second servo motor 9 installed on the movable frame 4, and an L-shaped plate 13 movably connected to the outer side of the movable frame 4, the outer side of the L-shaped plate 13 is provided with an open slot 14, the movable frame 4 is provided with a control system, the control system includes:

[0044] an image acquisition module 16 for capturing the image of the microfluidic chip 3 and its injection port and transmitting the image to an image processing module 17;

[0045] the image processing module 17 for processing the image collected by the image acquisition module 16, extracting the feature information of the injection port, and identifying the position and features of all injection ports on the microfluidic chip 3;

[0046] The image recognition module 18 matches the feature information extracted by the image processing module 17 with the preset injection port feature library through a feature matching algorithm to determine the identity of each injection port;

[0047] The position feedback and adjustment module 19 is configured to feed back the injection port position information selected by the user to the first servo motor 5 and the second servo motor 9, and control the first servo motor 5 and the second servo motor 9 to adjust the position of the L-shaped plate 13 through a path planning algorithm.

[0048] The human-computer interaction module 20 is configured to provide a user operation interface to allow the user to select a target injection port.

[0049] After the microfluidic chip 3 is placed in the movable plate 2, the image acquisition module 16 captures the image of the microfluidic chip 3 and its injection ports and transmits the image to the image processing module 17. The image processing module 17 pre-processes and detects the target of the image, identifies the position and features of all injection ports, and transmits the feature information to the image recognition module 18. The image recognition module 18 confirms the identity of the injection ports identified by the image processing module 17 and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module 20. The position feedback and adjustment module 19 feeds back the user's selection to the first servo motor 5 and the second servo motor 9. The first servo motor 5 and the second servo motor 9 are started to drive the L-shaped plate 13 to move in the horizontal position until the opening slot 14 corresponds to the position of the injection port. Then the pipette is inserted into the opening slot 14 for guidance and the liquid is dropped into the microfluidic chip 3. This can ensure that the pipette is more accurately aligned with the injection port of the microfluidic chip 3, avoiding slow positioning caused by manual operation, and also helping to reduce problems such as liquid splashing and drop position deviation caused by unstable operation.

[0050] The inner side of the movable frame 4 is rotatably connected with the first threaded rod 6 and the guide rod 7, respectively. The first threaded rod 6 is installed at the output shaft end of the first servo motor 5. The outer side of the guide rod 7 is slidably provided with the movable rod 8. The movable rod 8 is threadedly connected with the first threaded rod 6. When the first threaded rod 6 rotates, it drives the movable rod 8 to move along the guide rod 7.

[0051] The bottom of the movable rod 8 is provided with a square slot 15 and a sliding slot 10, respectively. The second servo motor 9 is installed at the inner side of the square slot 15. The inner side of the sliding slot 10 is slidably provided with a sliding block 12. The sliding block 12 moves along the sliding slot 10.

[0052] The end of the second servo motor 9 extending to the inner side of the sliding slot 10 is provided with the second threaded rod 11. The second threaded rod 11 is threadedly connected with the sliding block 12. The sliding block 12 is fixedly connected with the L-shaped plate 13. The second threaded rod 11 can drive the sliding block 12 to move along the sliding slot 10 by rotating.

[0053] The size of the sliding groove 10 opening is matched with the size of the sliding block 12, and the cross section of the sliding groove 10 and the sliding block 12 is convex, which ensures the stability of the sliding block 12.

[0054] The image acquisition module 16 includes an image capture unit and a data transmission unit. The image capture unit captures the image of the microfluidic chip 3 and its injection port through a camera and converts it into a digital signal. The data transmission unit transmits the captured image data to the image processing module 17 in real time.

[0055] The image processing module 17 includes an image preprocessing unit, a feature extraction unit, and a target detection unit. The image preprocessing unit performs denoising and enhancement preprocessing operations on the captured image. The feature extraction unit extracts the feature information of the injection port from the preprocessed image. The target detection unit identifies the positions and features of all injection ports on the microfluidic chip 3.

[0056] The image recognition module 18 includes a feature matching unit and a position accuracy correction unit. The feature matching unit matches the extracted feature information with the pre-set injection port feature library through a feature matching algorithm to determine the identity of each injection port. The position accuracy correction unit corrects the position of the matched injection port to improve the positioning accuracy.

[0057] The feature matching algorithm is the Euclidean distance, which is used to measure the similarity between two feature points. The smaller the Euclidean distance, the more similar the two feature points.

[0058] The calculation formula of the Euclidean distance is:

[0059]

[0060] Where f1 and f2 are the descriptors of two feature points, f 1i and f 2i is the i-th feature value, and n is the dimension of the feature vector.

[0061] The position feedback and adjustment module 19 includes a path planning unit, a control unit, and a position monitoring unit. The path planning unit calculates the moving path of the L-shaped plate 13 according to the position information of the target injection port through a path planning algorithm. The control unit controls the operation of the first servo motor 5 and the second servo motor 9. The position monitoring unit uses a position sensor to monitor the actual position of the L-shaped plate 13 in real time. The path planning algorithm is the A* algorithm, which is used to find the shortest path from the starting point to the target point in a graphical environment. The algorithm formula is:

[0062] Total path cost F(n): F(n) = G(n) + H(n);

[0063] G(n): actual cost from the starting point to the current node n;

[0064] H(n): estimated cost from the current node n to the target point.

[0065] In this embodiment, when detection work is needed, the microfluidic chip 3 is placed in the groove of the movable plate 2, then the movable frame 4 is rotated until the movable frame 4 is parallel to the movable plate 2, at this time the image acquisition module 16 can capture the image of the microfluidic chip 3 and its injection port, and the captured image is transmitted to the image processing module 17, the image processing module 17 identifies the position and characteristics of all injection ports and transmits the characteristic information to the image recognition module 18, then the image recognition module 18 confirms the identity of the injection port identified by the image processing module 17 and determines the accurate position of the target injection port, at this time the user can select the injection port that needs to be dripped according to the demand through the man-machine interaction module 20, the man-machine interaction module 20 transmits the selection information to the position feedback and adjustment module 19, the position feedback and adjustment module 19 drives the first servo motor 5 and the second servo motor 9 according to the injection port information selected by the user, and controls the first servo motor 5 and the second servo motor 9 to start;

[0066] The first servo motor 5 can drive the first threaded rod 6 to rotate when it starts, the force generated by the rotation of the first threaded rod 6 drives the movable rod 8 to move along the guide rod 7, and drives the L-shaped plate 13 to move, the second servo motor 9 can drive the second threaded rod 11 to rotate when it starts, the force generated by the rotation of the second threaded rod 11 drives the sliding block 12 to move along the sliding groove 10, thereby driving the L-shaped plate 13 to move, so that the L-shaped plate 13 can move horizontally until the opening slot 14 opened on the L-shaped plate 13 corresponds to the position of the injection port selected by the user, at this time the user can insert the pipette into the opening slot 14, the guide of the opening slot 14 can make the barrel of the pipette aim at the injection port of the microfluidic chip 3, then the dripping work can be carried out, after the dripping work is completed, the movable plate 2 can be pushed at this time, the movable plate 2 is pushed to the inside of the detection device 1, and then the detection work is carried out through the detection device 1.

[0067] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A microfluidic detection device, comprising a detection device (1), characterized in that, The detection device (1) is movably connected to a movable plate (2) on its side. A microfluidic chip (3) is disposed on the inner side of the movable plate (2). A movable frame (4) is rotatably connected to the upper part of the detection device (1). An adjustment mechanism is disposed on the movable frame (4). The adjustment mechanism includes a first servo motor (5) and a second servo motor (9) mounted on the movable frame (4), and an L-shaped plate (13) movably connected to the outer side of the movable frame (4). An opening slot (14) is provided on the outer side of the L-shaped plate (13). A control system is disposed on the movable frame (4). The control system includes: The image acquisition module (16) is used to capture images of the microfluidic chip (3) and its injection port and transmit them to the image processing module (17); The image processing module (17) is used to process the image acquired by the image acquisition module (16), extract the feature information of the injection port, and identify the position and features of all injection ports on the microfluidic chip (3). The image recognition module (18) uses a feature matching algorithm to match the feature information extracted by the image processing module (17) with a preset injection port feature library to determine the identity of each injection port. The position feedback and adjustment module (19) is used to feed back the injection port position information selected by the user to the first servo motor (5) and the second servo motor (9), and to control the first servo motor (5) and the second servo motor (9) to adjust the position of the L-shaped plate (13) through the path planning algorithm. The human-computer interaction module (20) is used to provide a user interface and allow users to select the target injection port; After the microfluidic chip (3) is placed in the movable plate (2), the image acquisition module (16) captures the image of the microfluidic chip (3) and its injection port and transmits it to the image processing module (17). The image processing module (17) preprocesses the image and performs target detection, identifies the position and features of all injection ports and transmits the feature information to the image recognition module (18). The image recognition module (18) confirms the identity of the injection port identified by the image processing module (17) and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module (20). The position feedback and adjustment module (19) feeds back the user's selection to the first servo motor (5) and the second servo motor (9) and starts them, driving the L-shaped plate (13) to move in a horizontal position until the opening groove (14) corresponds to the position of the injection port. Then, the pipette is inserted into the opening groove (14) for guidance and dripping liquid into the microfluidic chip (3).

2. The microfluidic detection device according to claim 1, characterized in that, The inner side of the movable frame (4) is rotatably connected to a first threaded rod (6) and a guide rod (7). The first threaded rod (6) is installed at the end of the output shaft of the first servo motor (5). A movable rod (8) is slidably arranged on the outer side of the guide rod (7). The movable rod (8) is threadedly connected to the first threaded rod (6).

3. The microfluidic detection device according to claim 2, characterized in that, The bottom of the movable rod (8) is provided with a square groove (15) and a sliding groove (10). The second servo motor (9) is installed on the inner side of the square groove (15), and a sliding block (12) is slidably arranged on the inner side of the sliding groove (10).

4. A microfluidic detection device according to claim 3, characterized in that, The second servo motor (9) extends to one end inside the sliding groove (10) and is fitted with a second threaded rod (11). The second threaded rod (11) is threadedly connected to the sliding block (12), and the sliding block (12) is fixedly connected to the L-shaped plate (13).

5. A microfluidic detection device according to claim 4, characterized in that, The size of the opening of the sliding groove (10) is adapted to the size of the sliding block (12), and the cross-sections of the sliding groove (10) and the sliding block (12) are convex.

6. The microfluidic detection device according to claim 1, characterized in that, The image acquisition module (16) includes an image capture unit and a data transmission unit. The image capture unit captures images of the microfluidic chip (3) and its injection port through a camera and converts them into digital signals. The data transmission unit transmits the captured image data to the image processing module (17) in real time.

7. A microfluidic detection device according to claim 1, characterized in that, The image processing module (17) includes an image preprocessing unit, a feature extraction unit, and a target detection unit. The image preprocessing unit performs noise reduction and enhancement preprocessing operations on the captured image. The feature extraction unit extracts the feature information of the injection port from the preprocessed image. The target detection unit identifies the position and features of all injection ports on the microfluidic chip (3).

8. A microfluidic detection device according to claim 1, characterized in that, The image recognition module (18) includes a feature matching unit and a position accuracy correction unit. The feature matching unit matches the extracted feature information with a preset injection port feature library through a feature matching algorithm to determine the identity of each injection port. The position accuracy correction unit performs accuracy correction on the matched injection port position. The feature matching algorithm is Euclidean distance, which is used to measure the similarity between two feature points. The smaller the Euclidean distance, the more similar the two feature points are. The formula for calculating Euclidean distance is: Where f1 and f2 are descriptors for two feature points, f 1i and f 2i is the i-th eigenvalue, and n is the dimension of the eigenvector.

9. A microfluidic detection device according to claim 1, characterized in that, The position feedback and adjustment module (19) includes a path planning unit, a control unit, and a position monitoring unit. The path planning unit calculates the movement path of the L-shaped plate (13) based on the position information of the target injection port using a path planning algorithm. The control unit controls the operation of the first servo motor (5) and the second servo motor (9). The position monitoring unit uses a position sensor to monitor the actual position of the L-shaped plate (13) in real time. The path planning algorithm is the A* algorithm, which is used to find the shortest path from the starting point to the target point in the graphical environment. Its algorithm formula is: Total path cost F(n): F(n) = G(n) + H(n); G(n): The actual cost of getting from the starting point to the current node n; H(n): Estimated cost from the current node n to the target node.

10. A method using a microfluidic detection device, employing the microfluidic detection device of claim 1, characterized in that, Includes the following steps: Step 1: First, pull the movable plate (2) to move it to the outside of the detection device (1). Then, put the microfluidic chip (3) into the groove of the movable plate (2). Then, rotate the movable frame (4) until the movable frame (4) is parallel to the movable plate (2). Step 2: The image acquisition module (16) captures images of the microfluidic chip (3) and its injection port. Then, the image processing module (17) identifies the position and features of all injection ports. After that, the image recognition module (18) determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module (20) according to the requirements. The position feedback and adjustment module (19) feeds back the position information of the injection port selected by the user to the first servo motor (5) and the second servo motor (9). Step 3: After receiving the signal, the first servo motor (5) and the second servo motor (9) start up and drive the L-shaped plate (13) to move in a horizontal position until the opening groove (14) corresponds to the position of the injection port. Then, the staff inserts the pipette into the opening groove (14) for guidance and drips the liquid into the microfluidic chip (3). After the dripping is completed, the movable plate (2) is closed and the detection device (1) is used for detection.

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