Double-laser liquid drop control measurement method and device

Abstract

The invention provides a double-laser liquid drop control measurement method and device.The double-laser liquid drop control measurement method comprises the steps that nano-particle liquid drops are prepared, and the nano-particle liquid drops are placed on a glass substrate of a pre-configured liquid drop operation table; laser is emitted through the liquid drop operation table, laser parameters are adjusted, the laser is made to be focused in the nano-particle liquid drops, and the nano-particle liquid drops are driven to move; raman laser penetrating through the nano-particle liquid drops is emitted through the liquid drop operation table, and the internal characteristics and the movement condition of the nano-particle liquid drops are determined according to the imaging result of the Raman laser; the internal characteristics include real-time temperature and structural characteristics. By means of the method and device, accurate control over liquid drop fluid flowing is achieved, the movement condition of nano-particle liquid drops can be fed back in real time in the control process, and the problem that in the prior art, the liquid drop control accuracy degree is poor is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of droplet manipulation, and in particular, to a dual-laser droplet manipulation and measurement method and device. Background Art

[0002] Droplet manipulation is crucial for scientific research and practical applications, and is of great significance in fields such as precision medicine, biosensing, and 3D printing. In most cases, the manipulation of droplets is achieved by using microfluidic devices. These microfluidic devices often have complex structures, high costs, and when performing complex operations on micro-volume liquids in a parallel and high-throughput manner, the contamination of sensors will also limit the functions of the devices. In addition, classical microfluidic devices usually have a high degree of specificity for a given operation, and their universality is poor.

[0003] Compared with other droplet driving methods, light driving has advantages such as non-contact driving and adjustable excitation, and is an extremely suitable droplet driving method. However, the existing droplet driving methods are difficult to adjust the movement of droplets in a timely manner during the droplet control process, and the accuracy of the droplet driving process is not ideal.

[0004] For the problem of poor accuracy in droplet manipulation existing in the existing related technologies, no effective solution has been proposed yet. Summary of the Invention

[0005] The present invention provides a dual-laser droplet manipulation and measurement method and device to solve the defect of poor accuracy in droplet manipulation existing in the existing related technologies, and achieve precise manipulation of droplet fluid flow.

[0006] In a first aspect, the present invention provides a dual-laser droplet manipulation and measurement method, including: Preparing a nanoparticle droplet and placing the nanoparticle droplet on a glass substrate of a pre-configured droplet operation table; Emitting a laser through the droplet operation table and adjusting the laser parameters to focus the laser inside the nanoparticle droplet to drive the nanoparticle droplet to move; Emitting Raman laser passing through the nanoparticle droplet through the droplet operation table, and determining the internal characteristics and movement of the nanoparticle droplet through the imaging result of the Raman laser; the internal characteristics include real-time temperature and structural characteristics; Based on the internal characteristics and movement of the nanoparticle droplet, adjusting the laser parameters of the laser to control the movement of the nanoparticle droplet.

[0007] According to the dual-laser droplet manipulation and measurement method provided by the present invention, the size of the nanoparticles in the nanoparticle droplet is 60 - 80 nm.

[0008] A dual-laser droplet manipulation and measurement method provided by the present invention emits a laser through the droplet operation platform and adjusts the laser parameters to focus the laser inside the nanoparticle droplet and drive the movement of the nanoparticle droplet, including: Adjust the energy density, polarization state, wavelength, and incident position of the laser to focus the laser inside the nanoparticle droplet; Heat the nanoparticle droplet through the laser, causing the nanoparticle droplet to be locally heated and generate a temperature gradient and nanoparticle bubbles, and pushing the nanoparticle droplet to move.

[0009] A dual-laser droplet manipulation and measurement method provided by the present invention, wherein the wavelength range of the laser emitted by the droplet operation platform is 400 - 700 nm, the pulse time is 5 - 20 ps, the period is 10 - 30 ns, and the laser power adjustment range is 0.35×10 6 W / cm 2 -10 6 W / cm 2 .

[0010] A dual-laser droplet manipulation and measurement method provided by the present invention, wherein the wavelength range of the Raman laser emitted by the droplet operation platform is 800 - 1200 nm, and the laser power adjustment range is 0.35×10 5 W / cm 2 -10 5 W / cm 2 .

[0011] A dual-laser droplet manipulation and measurement method provided by the present invention determines the internal characteristics and movement conditions of the nanoparticle droplet through the imaging result of the Raman laser, including: Collect the Raman spectrum of the Raman laser, and based on the peak, full width at half maximum, and intensity of the Raman spectrum, combine with the nanoparticle Raman shift coefficient to determine the real-time temperature and surface structure characteristics of the local hot spot of the nanoparticle droplet; Record the movement image of the nanoparticle bubbles inside the nanoparticle droplet based on the Raman laser, and determine the movement conditions of the nanoparticle droplet according to the position change of the nanoparticle bubbles.

[0012] A dual-laser droplet manipulation and measurement method provided by the present invention adjusts the laser parameters of the laser based on the internal characteristics and movement conditions of the nanoparticle droplet to control the movement of the nanoparticle droplet, including: Based on the internal characteristics and movement conditions of the nanoparticle droplet, adjust the laser power, wavelength of the laser, or the position of the nanoparticle droplet to control the movement of the nanoparticle droplet.

[0013] Second aspect, the present invention also provides a dual-laser droplet manipulation and measurement device for implementing the dual-laser droplet manipulation and measurement method described in the first aspect, including a droplet operation table and an electronic control device, the droplet operation table is connected to the electronic control device and is controlled by the electronic control device; The droplet operation table includes a manipulation module and a measurement module; The manipulation module is used to place the nanoparticle droplet and release laser to drive the movement of the nanoparticle droplet; The measurement module is used to release Raman laser to determine the internal characteristics and movement of the nanoparticle droplet.

[0014] According to a dual-laser droplet manipulation and measurement device provided by the present invention, the manipulation module includes a displacement stage, and a glass substrate is arranged on the displacement stage; The operation module further includes a laser, a short-wave cut-off filter, a dichroic mirror and a first objective lens; The laser released by the laser passes through the short-wave cut-off filter and shoots towards the dichroic mirror, is reflected by the dichroic mirror to the first objective lens, and is focused and magnified by the first objective lens and shoots towards the glass substrate.

[0015] According to a dual-laser droplet manipulation and measurement device provided by the present invention, the measurement module includes a Raman laser, a second objective lens, a beam splitter, a high-speed imager and a Raman spectrometer; The Raman laser released by the laser passes through the second objective lens and irradiates towards the glass substrate, passes through the glass substrate and the dichroic mirror, and after passing through the beam splitter, enters the high-speed imager and the Raman spectrometer respectively; The high-speed imager is used to record the movement images of the nanoparticle bubbles inside the nanoparticle droplet; The Raman spectrometer is used to collect the Raman spectrum of the Raman laser.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The dual-laser droplet manipulation and measurement method provided by the present invention, through the drive of the laser, the nanoparticle bubbles carry the nanoparticle droplet and move together with the laser, realizing precise manipulation of the droplet fluid flow, and being able to provide real-time feedback on the movement of the nanoparticle droplet during the manipulation process, facilitating timely correction of the movement path of the nanoparticle droplet, and solving the problem of poor precision in droplet manipulation existing in the existing related technologies. Description of the Drawings

[0017] To more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 is a flowchart of the dual-laser droplet manipulation and measurement method provided by the present invention; Figure 2 is a schematic diagram of in-situ monitoring of nanoparticle droplets in an embodiment of the present invention; Figure 3 is a schematic structural diagram of the dual-laser droplet manipulation and measurement device provided by the present invention.

[0019] Reference numerals: 1: Raman laser; 2: short-wave cut-off filter; 3: first objective lens; 4: displacement stage; 5: nanoparticle droplet; 6: glass substrate; 7: laser; 8: Raman laser; 9: beam splitter; 10: dichroic mirror; 11: high-speed imager; 12: Raman spectrometer; 13: second objective lens. Detailed implementation manners

[0020] To make the objectives, technical solutions, and advantages of the present invention clearer, the following will clearly and completely describe the technical solutions in the present invention in conjunction with the drawings in the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts based on the embodiments in the present invention belong to the scope of protection of the present invention.

[0021] Plasmonics is based on the interaction process between electromagnetic radiation and conduction electrons at the metal interface or in sub-wavelength-sized metal structures. This interaction can confine light to a very small size, thereby leading to enhanced optical near-field and optical nonlinear effects at the sub-wavelength scale, and realizing the manipulation of light fields and thermal fields at the nanoscale. Inside the fluid, the plasmonic effect of the laser and metal particles will generate high-temperature supercavitation on the surface of the metal particles and move directionally under the action of the laser light thrust, thereby driving the droplet to move and realizing droplet manipulation.

[0022] Raman spectroscopy is a scattering spectroscopy and also a vibrational spectroscopy technique. When light irradiates a substance, elastic scattering and inelastic scattering occur. The scattered light of elastic scattering has the same wavelength as the excitation light. The inelastic scattered light has components longer and shorter than the excitation light wavelength, which are collectively called the Raman effect. An analytical method for analyzing the scattering spectrum different from the incident light frequency to obtain information on molecular vibration and rotation and applying it to the study of molecular structure.

[0023] Based on this, the present invention provides a dual-laser droplet manipulation and measurement method. Figure 1 It is a flowchart of the dual-laser droplet manipulation and measurement method provided by the present invention. As Figure 1 shown, the method includes the following steps: Step S101: Prepare nanoparticle droplets and place the nanoparticle droplets on the glass substrate of a pre-configured droplet operation table. Step S102: Emit a laser through the droplet operation table and adjust the laser parameters to focus the laser inside the nanoparticle droplets, driving the movement of the nanoparticle droplets. Step S103: Emit Raman laser passing through the nanoparticle droplets through the droplet operation table, and determine the internal characteristics and movement conditions of the nanoparticle droplets based on the imaging results of the Raman laser; the internal characteristics include the real-time temperature and structural characteristics. Step S104: Based on the internal characteristics and movement conditions of the nanoparticle droplets, adjust the laser parameters of the laser to control the movement of the nanoparticle droplets.

[0024] Exemplarily, first, prepare nanoparticle droplets and use a pipette to drop them on the glass substrate, and adjust the droplet operation table to complete the preparations before the experiment. Among them, the size of the nanoparticles in the nanoparticle droplets is 60 - 80 nm. The nanoparticle droplets can resonate with the laser emitted by the laser, generate the plasmon effect, and can perform Raman signal structure modification to generate obvious Raman signals under the action of the Raman laser, such as a gold nanoparticle sodium citrate solution). Then, generate Gaussian laser (Gaussian laser is a pulsed laser) through the droplet operation table, and adjust the laser parameters of the Gaussian laser to accurately focus the laser inside the nanoparticle droplets, thereby heating the nanoparticle droplets and driving the movement of the nanoparticle droplets. During the movement of the nanoparticle droplets, emit Raman laser passing through the nanoparticle droplets through the droplet operation table, and determine the internal characteristics and movement conditions of the nanoparticle droplets based on the imaging results of the Raman laser to understand the movement process of the nanoparticle droplets in real time. During this process, based on the feedback movement process, adjust the laser parameters of the Gaussian laser to adjust and correct the movement path or movement conditions of the nanoparticle droplets. In the above process, under the drive of the laser, the nanoparticle bubbles carry the nanoparticle droplets and move together with the laser, realizing precise control of the droplet fluid flow, and being able to provide real-time feedback on the movement conditions of the nanoparticle droplets during the control process, facilitating timely correction of the movement path of the nanoparticle droplets, and solving the problem of poor precision in droplet manipulation existing in the existing related technologies.

[0025] In some of these embodiments, in step S102, a laser is emitted by a droplet operation platform, and the laser parameters are adjusted to focus the laser inside the nanoparticle droplet to drive the movement of the nanoparticle droplet, including: adjusting the energy density, polarization state, wavelength, and incident position of the laser to focus the laser inside the nanoparticle droplet; heating the nanoparticle droplet by the laser to locally heat the nanoparticle droplet and generate a temperature gradient and nanoparticle bubbles to push the nanoparticle droplet to move.

[0026] Specifically, the movement of the nanoparticle droplet is affected by two factors. One is that inside the fluid, the plasmon effect between the laser and the particle will generate high-temperature supercavitation on the particle surface to form nanoparticle bubbles, and under the action of the optical thrust of the laser, it will move directionally, thereby driving the movement of the nanoparticle droplet to achieve droplet manipulation. The other is that the plasmon effect between the laser and the particle will generate high temperature on the particle surface, and the local heating inside the nanoparticle droplet will generate a temperature gradient, changing the surface tension of the nanoparticle droplet, causing the nanoparticle droplet to move and achieving droplet manipulation.

[0027] In some of these embodiments, the wavelength range of the laser emitted by the droplet operation platform is 400 - 700 nm. The specific wavelength selected depends on the type and size of the nanoparticle droplet. The pulse time is 5 - 20 ps, the period is 10 - 30 ns, and the laser power adjustment range is 0.35×10 6 W / cm 2 -10 6 W / cm 2 . Correspondingly, the wavelength range of the Raman laser emitted by the droplet operation platform is 800 - 1200 nm, and the laser power adjustment range is 0.35×10 5 W / cm 2 -10 5 W / cm 2 . The laser with a wavelength range of 400 - 700 nm can resonate with the nanoparticles to produce a plasmon effect. The laser with a power adjustment range of 0.35×10 6 W / cm 2 -10 6 W / cm 2 can cause the nanoparticles to generate bubbles ranging from micrometers to millimeters and locally generate a temperature gradient of 40 - 80 K inside the droplet, thereby driving the droplet to move.

[0028] Figure 2 is a schematic diagram for in-situ monitoring of the nanoparticle droplet in the embodiments of the present invention, as shown in Figure 2As shown, in some of these embodiments, in-situ monitoring of nanoparticle droplets is performed based on Raman laser. Specifically, in step S103, the internal characteristics and motion of the nanoparticle droplets are determined through the imaging results of the Raman laser, including: collecting the Raman spectrum of the Raman laser, and based on the peak value, full width at half maximum, and intensity of the Raman spectrum, combining with the Raman displacement coefficient of the nanoparticles to determine the real-time temperature of the local hot spots of the nanoparticle droplets and the structural characteristics of the surface; recording the motion images of the nanoparticle bubbles inside the nanoparticle droplets based on the Raman laser, and determining the motion of the nanoparticle droplets according to the position changes of the nanoparticle bubbles.

[0029] Based on the above embodiments, in step S104, based on the internal characteristics and motion of the nanoparticle droplets, the laser parameters of the laser are adjusted to control the motion of the nanoparticle droplets, including: based on the internal characteristics and motion of the nanoparticle droplets, adjusting the laser power, wavelength, or the position of the nanoparticle droplets of the laser to control the motion of the nanoparticle droplets. In addition, the motion of the nanoparticle droplets can also be controlled by adjusting the energy density, polarization state, incident angle, etc. of the laser.

[0030] In summary, this method uses a laser to achieve precise manipulation of droplets. Under the heating of the laser, the nanoparticles become gasification cores to generate nanoparticle bubbles, and a large number of nanoparticles are distributed inside the droplets. Driven by the laser, the nanoparticle bubbles carry the gold nanoparticles and move together with the laser to achieve precise manipulation of the fluid flow of the nanoparticle droplets. Moreover, this method combines droplet driving, Raman detection technology, and high-speed imaging technology. While realizing droplet optical drive, it can detect the temperature data and motion of the cavitation bubbles of the gold nanoparticles inside the nanoparticle droplets in real time, and can timely adjust the flow of the nanoparticle droplets.

[0031] The present invention also provides a dual-laser droplet manipulation and measurement device. The dual-laser droplet manipulation and measurement device provided by the present invention will be described below. The dual-laser droplet manipulation and measurement device described below can be mutually corresponding and referred to the dual-laser droplet manipulation and measurement method described above. Figure 3 is a schematic structural diagram of the dual-laser droplet manipulation and measurement device provided by the present invention. As Figure 3 shown, the device includes a droplet operation table and an electronic control device. The droplet operation table is connected to the electronic control device and is controlled by the electronic control device; The droplet operation table includes a manipulation module and a measurement module; The manipulation module is used to place the nanoparticle droplet 5 and release the laser to drive the motion of the nanoparticle droplet 5; The measurement module is used to release the Raman laser to determine the internal characteristics and motion of the nanoparticle droplet 5.

[0032] Specifically, the manipulation module includes a displacement stage 4, on which a glass substrate 6 is provided; the operation module further includes a laser 7, a short-wave cut-off filter 2, a dichroic mirror 10 and a first objective lens 3. The laser 7 emits laser light that passes through the short-wave cut-off filter 2 and is directed towards the dichroic mirror 10, is reflected by the dichroic mirror 10 to the first objective lens 3, and is focused and magnified by the first objective lens 3 and directed towards the glass substrate 6. Among them, the dichroic mirror 10 reflects pulsed laser light and allows Raman laser light to pass through.

[0033] During the manipulation process, the prepared nanoparticle droplet 5 is placed on the glass substrate 6 by a pipette. The laser 7 emits laser light that passes through the short-wave cut-off filter 2 and the dichroic mirror 10 in sequence and irradiates vertically from above the droplet, and is focused inside the nanoparticle droplet 5 after passing through the first objective lens 3. The magnification of the first objective lens 3 is 100 times, and the radius of the laser spot after being focused by the first objective lens 3 is 0.5 - 2 µm. The laser can generate a surface plasmon resonance effect with the nanoparticles. By adjusting the energy density of the laser 7 and the laser focusing position, a temperature gradient is generated by local heating inside the nanoparticle droplet 5, thereby manipulating the movement of the nanoparticle droplet 5. In addition, the displacement stage 4 is connected to an electronic control device, and the electronic control device can be a device such as a computer. During the above operation process, the movement of the nanoparticle droplet 5 near the laser position can also be controlled by the displacement stage 4, so that the laser is always focused on the edge position of the nanoparticle droplet 5, and the adjustment accuracy is 10 nm.

[0034] The measurement module includes a Raman laser 1, a second objective lens 13, a beam splitter 9, a high-speed imager 11 and a Raman spectrometer 12. The Raman laser 1 emits Raman laser light that passes through the second objective lens 13 and irradiates towards the glass substrate 6, passes through the glass substrate 6 and the dichroic mirror 10, and after passing through the beam splitter 9, enters the high-speed imager 11 and the Raman spectrometer 12 respectively. The high-speed imager 11 is used to record the movement images of the nanoparticles and bubbles inside the nanoparticle droplet 5; the Raman spectrometer 12 is used to collect the Raman spectra of the Raman laser light.

[0035] During the measurement process, the Raman laser 1 is arranged directly below the nanoparticle droplet 5, and the Raman laser light 8 generated by the Raman laser 1 irradiates the droplet vertically from below. The Raman spectrometer 12 and the high-speed imager 11 are located directly above the nanoparticle droplet 5. The Raman laser light 8 passing through the nanoparticle droplet 5 passes through the dichroic mirror 10 and then through the beam splitter 9 and enters the high-speed imager 11 and the Raman spectrometer 12 respectively. The high-speed imager 11 can analyze the movement of the nanoparticles and bubbles inside the nanoparticle droplet 5 in real time, and the Raman spectrometer 12 can detect Raman signals in real time and analyze the temperature information and structural characteristics of the nanoparticles in the nanoparticle droplet 5.

[0036] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A dual-laser droplet manipulation and measurement method, characterized in that Comprising: Preparing nanoparticle droplets and placing the nanoparticle droplets on a glass substrate of a pre-configured droplet operation table; Emitting a laser through the droplet operation table and adjusting laser parameters to focus the laser inside the nanoparticle droplets to drive the movement of the nanoparticle droplets; Emitting Raman laser passing through the nanoparticle droplets through the droplet operation table and determining the internal characteristics and movement conditions of the nanoparticle droplets based on the imaging results of the Raman laser; the internal characteristics include real-time temperature and structural characteristics; Adjusting the laser parameters of the laser based on the internal characteristics and movement conditions of the nanoparticle droplets to control the movement of the nanoparticle droplets.

2. The dual-laser droplet manipulation and measurement method according to claim 1, characterized in that The size of the nanoparticles in the nanoparticle droplets is 60 - 80 nm.

3. The dual-laser droplet manipulation and measurement method according to claim 1, wherein Emitting a laser through the droplet operation table and adjusting laser parameters to focus the laser inside the nanoparticle droplets to drive the movement of the nanoparticle droplets, including: Adjusting the energy density, polarization state, wavelength, and incident position of the laser to focus the laser inside the nanoparticle droplets; Heating the nanoparticle droplets by the laser to locally heat the nanoparticle droplets and generate a temperature gradient and nanoparticle bubbles to push the movement of the nanoparticle droplets.

4. The dual-laser droplet manipulation and measurement method according to claim 1, wherein The wavelength range of the laser emitted by the droplet operation table is 400 - 700 nm, the pulse time is 5 - 20 ps, the period is 10 - 30 ns, and the laser power adjustment range is 0.35×10 6 W / cm 2 -10 6 W / cm 2 .

5. The dual-laser droplet manipulation and measurement method according to claim 1, characterized in that The wavelength range of the Raman laser emitted by the droplet operating table is 800 - 1200 nm, and the laser power adjustment range is 0.35×10 5 W / cm 2 -10 5 W / cm 2 .

6. The dual-laser droplet manipulation and measurement method according to claim 1, characterized in that Determining the internal characteristics and movement conditions of the nanoparticle droplets based on the imaging results of the Raman laser, including: Collecting the Raman spectrum of the Raman laser, and determining the real-time temperature and surface structural characteristics of the local hot spots of the nanoparticle droplets based on the peak value, full width at half maximum, and intensity of the Raman spectrum, in combination with the Raman displacement coefficient of the nanoparticles; Recording the movement images of the nanoparticle bubbles inside the nanoparticle droplets based on the Raman laser, and determining the movement conditions of the nanoparticle droplets according to the position changes of the nanoparticle bubbles.

7. The dual-laser droplet manipulation and measurement method according to claim 1, wherein Adjusting the laser parameters of the laser based on the internal characteristics and movement conditions of the nanoparticle droplets to control the movement of the nanoparticle droplets, including: Adjusting the laser power, wavelength of the laser, or the position of the nanoparticle droplets based on the internal characteristics and movement conditions of the nanoparticle droplets to control the movement of the nanoparticle droplets.

8. A dual-laser droplet manipulation and measurement device for implementing the dual-laser droplet manipulation and measurement method according to any one of claims 1-7, characterized in that, Including a droplet operation table and an electronic control device, the droplet operation table is connected to the electronic control device and is controlled by the electronic control device; The droplet operation table includes a control module and a measurement module; The control module is used to place the nanoparticle droplets (5) and release a laser to drive the movement of the nanoparticle droplets (5); The measurement module is used to release Raman laser to determine the internal characteristics and movement conditions of the nanoparticle droplets (5).

9. The dual-laser droplet manipulation and measurement device according to claim 8, wherein, The control module includes a displacement stage (4), and a glass substrate (6) is arranged on the displacement stage (4); The operation module further includes a laser (7), a short-wave cut-off filter (2), a dichroic mirror (10), and a first objective lens (3); The laser released by the laser (7) passes through the short-wave cut-off filter (2) and shoots towards the dichroic mirror (10), is reflected by the dichroic mirror (10) to the first objective lens (3), and is focused and magnified by the first objective lens (3) and shoots towards the glass substrate (6).

10. The dual-laser droplet manipulation and measurement device according to claim 8, wherein, The measurement module includes a Raman laser (1), a second objective lens (13), a beam splitter (9), a high-speed imager (11), and a Raman spectrometer (12); The Raman laser released by the laser (1) passes through the second objective lens (13) and irradiates the glass substrate (6), passes through the glass substrate (6) and the dichroic mirror (10), and then enters the high-speed imager (11) and the Raman spectrometer (12) respectively after passing through the beam splitter (9); The high-speed imager (11) is used to record the motion images of the nanoparticle bubbles inside the nanoparticle droplet (5); The Raman spectrometer (12) is used to collect the Raman spectra of the Raman laser.

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