Immune cell detector and detection method thereof

Abstract

The present invention relates to the field of cell detection technology, and discloses an immune cell detector and a detection method thereof, comprising a reaction disk, a plurality of clamping racks arranged in a circular array on the reaction disk, a reaction cup placed in the clamping rack, a coaxial enclosure mounted on the outside of the reaction disk, a cylinder arranged in the detection direction of the enclosure, a detection clamping plate fixedly mounted on the telescopic end of the cylinder, a car frame rotatably mounted on the enclosure, the detection clamping plate and the car frame intermittently engaged, a housing arranged in the center of the reaction disk, a hydraulic cylinder mounted on the housing, an active clamping plate arranged at the telescopic end of the hydraulic cylinder, the active clamping plate and the detection clamping plate being arranged correspondingly, a striking plate rotatably mounted on the housing, and a striking assembly also being arranged on the housing. This solution can detect different stages, automatically realize the transfer, clamping, detection in a stable state, and automatic unloading of the reaction cup, making the detection more automated.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and in particular to an immune cell detector and a detection method thereof. Background Art

[0002] In related technologies, immunity testing primarily assesses immune system function by measuring blood indicators. These indicators include white blood cell count, lymphocyte count, and levels of various immunoglobulins. During the test, a reaction reagent is mixed with a body fluid sample in a cuvette. The cuvette then reacts in a heated and insulated environment for a period of time before being transported to the testing location for photoelectric conversion testing.

[0003] In immunoassay analyzers, disposable cuvettes are used as test consumables and need to be replenished and removed. They are usually placed in cuvette receiving grooves distributed along the circumference of the reaction disk. During the luminescence detection process, the tested cuvette needs to be automatically moved to the detection area and then automatically removed after the detection is completed. Since chemiluminescence relies on the PMT to accurately capture transient light signals at a fixed position, luminescence detection requires high position accuracy of the cuvette. Therefore, better positioning, stable detection and automatic unloading of the cuvette are required. Therefore, we propose an immune cell detector and a detection method thereof. Summary of the Invention

[0004] The present invention provides an immune cell detector and a detection method thereof, which can detect different stages and automatically realize the transfer, clamping, detection in a stable state and automatic unloading of the reaction cup, making the detection more automated, and solving the problem of better positioning, stable detection and automatic unloading of the reaction cup mentioned in the above background technology.

[0005] In order to achieve the above-mentioned purpose, the present disclosure provides an immune cell detector, including a reaction disk, a plurality of clamping frames are arranged in a circular array on the reaction disk, a reaction cup is placed in the clamping frame, a surrounding plate is coaxially installed on the outer side of the reaction disk, a cylinder is provided in the detection direction of the surrounding plate, a detection clamping plate is fixedly installed on the telescopic end of the cylinder, a car frame is rotatably installed on the surrounding plate, and the detection clamping plate is intermittently engaged with the car frame, a shell is provided in the center of the reaction disk, a hydraulic cylinder is installed on the shell, an active clamping plate is provided at the telescopic end of the hydraulic cylinder, the active clamping plate is arranged corresponding to the detection clamping plate, a striking plate is rotatably provided on the shell, and a striking assembly is also provided on the shell, the active clamping plate and the detection clamping plate clamp the reaction cup until the detection is completed, and the striking plate drives the cylinder to operate through the striking assembly to separate the detection clamping plate from the active clamping plate.

[0006] Optionally, a plurality of hollow portions are formed in a circumferential array on the reaction disk, and the clamping frame is hinged in the hollow portions. The clamping frame is configured as an arc-shaped clamping hand with a silicone head as a clamping end.

[0007] Optionally, a fixed sleeve is installed on the side of the active clamping plate, and an elastic sleeve is fixedly installed on the telescopic end of the hydraulic cylinder. The elastic sleeve is plugged and engaged with the fixed sleeve, and the elastic sleeve is configured as a sleeve with a spring inside.

[0008] Optionally, a synchronization rod is installed on the side of the fixed sleeve, a reinforcement seat is installed on the side of the housing, the striking plate is hingedly mounted on the reinforcement seat, a guide slot is opened on the striking plate, and the synchronization rod passes through the guide slot;

[0009] The guide groove includes a horizontal groove and an arcuate groove, and the arcuate groove is connected to a side of the horizontal groove adjacent to the reaction cup;

[0010] In normal state, the synchronization rod is located in the horizontal through groove;

[0011] During detection, the synchronization rod is located in the arc-shaped groove.

[0012] Optionally, a piston cylinder is installed on the casing, and a return air pipe and an exhaust pipe are connected to the side of the piston cylinder. The return air pipe and the exhaust pipe are connected to the cylinder, and the striking plate is intermittently connected to the piston cylinder.

[0013] Optionally, the striking assembly includes a fixed rack fixedly mounted on the casing, a struck block slidably mounted in the casing, and a piston plate doing work in the piston cylinder, a double-tooth rack is slidingly provided on the side of the fixed rack, a connecting block is installed on the side of the striking block, a double-gear with a distance is rotatably installed on the side of the connecting block, the double-gear with a distance is simultaneously engaged with the double-tooth rack and the fixed rack, an output rack is installed on the side of the piston plate, a transmission gear is rotatably installed inside the casing, the transmission gear is simultaneously engaged with the output rack and the double-tooth rack, and a reset spring for reset is installed between the striking block and the casing.

[0014] Optionally, a fixing frame is provided in the detection direction of the reaction disc, the cylinder is fixedly mounted on the fixing frame, a material unloading frame is fixedly mounted in the unloading direction of the fixing frame, and the material unloading frame is intermittently docked with the car frame;

[0015] An output wheel shaft is rotatably mounted on the end of the synchronization rod, and the output wheel shaft is clearance-matched with the horizontal through slot. An axle end wheel is fixedly mounted on the end of the output wheel shaft, and a return rod is mounted on the side of the axle end wheel.

[0016] An L-shaped groove is provided on the casing, a reversing plate is slidably installed in the L-shaped groove, the reversing plate is vertically connected to an L-shaped rod, the reversing plate and the reversing rod are intermittently matched, and the L-shaped rod is transmission-connected to the car frame.

[0017] Optionally, a blanking shaft is rotatably mounted on the enclosure, the car frame is fixedly mounted on the blanking shaft, a driving rod is rotatably mounted on the casing, a pulley set is commonly mounted between the blanking shaft and the driving rod, a gear roller is coaxially mounted on the blanking shaft, a transverse rack is connected to the end of the L-shaped rod, and the transverse rack is engaged with the gear roller;

[0018] When the reversing plate rotates and drives the reversing rod to move, the car frame rotates and docks with the unloading rack.

[0019] Optionally, a pressure spring is installed between the reversing plate and the L-shaped groove wall, a corresponding notch is provided at one end of the car frame adjacent to the detection clamping plate, and a docking notch is provided at the corresponding end of the detection clamping plate.

[0020] According to a second aspect of the present disclosure, a method for detecting immune cells is provided, using the immune cell detector as described above, comprising the following specific steps:

[0021] S1. Sample Collection and Pretreatment: Collect biological samples and pre-treat them by filtering them through a 5-10 micron pore size filter to remove impurities and large cell aggregates. The filtered samples are then centrifuged at 1500-2000 rpm for 5-10 minutes to separate the supernatant containing immune cells.

[0022] S2. Immune cell labeling: Add specific immune cell markers to the supernatant, including fluorescein-labeled antibodies and magnetic bead-labeled antibodies. Incubate the supernatant with the markers at 37°C for 15-30 minutes to allow the markers to fully bind to the immune cells.

[0023] S3. The incubated sample is treated with a magnetic field, causing the magnetic bead-labeled immune cells to be adsorbed on one side of the magnetic field, thereby separating them from other components. The immune cells adsorbed on one side of the magnetic field are then eluted with an eluent, thereby enriching the immune cells.

[0024] S4. Place the enriched immune cell sample in a reaction cup and place it on a reaction plate. Use excitation light of different wavelengths to stimulate the fluorescein-labeled antibodies on the immune cells, detect the fluorescent signals emitted by the immune cells, and transmit the acquired fluorescent signal data to the data processing system. The data processing system uses a deep learning-based algorithm model to process and analyze the data, and generates an immune cell analysis and detection report based on the analysis results. The report content includes information such as the type, number, proportion, and various characteristic parameters of the immune cells.

[0025] Through the above technical solution, the immune cell detector and the detection method provided by the present disclosure are used: through the setting of the synchronization rod, during the operation of the hydraulic cylinder, the synchronization rod moves in the guide groove, thereby driving the striking plate to rotate, thereby triggering the striking assembly, and utilizing the transmission of the striking assembly to trigger the piston cylinder and the cylinder, so that when the detection is completed, the synchronization rod is located at the arc peak of the arc-shaped groove. At this time, the striking assembly does the maximum work, so that the detection clamping plate is driven upward by the cylinder, thereby realizing the separation of the detection clamping plate and the active clamping plate. At this time, the detection has ended, the reaction cup loses the support of the detection clamping plate, and the spring in the elastic sleeve is released, so that the reaction cup is pushed down by the active clamping plate to realize automatic unloading;

[0026] This solution can detect different stages through the cooperation of the synchronization rod, the striking plate, and the guide groove, and automatically realize the transfer, clamping, and automatic unloading of the reaction cup in a stable state, making the detection more automated, ensuring the detection accuracy, and improving the efficiency of immune cell detection.

[0027] Other features and advantages of the present disclosure will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The accompanying drawings are used to provide a further understanding of the present disclosure and constitute a part of the specification. Together with the following detailed description, they are used to explain the present disclosure but do not constitute a limitation of the present disclosure. In the accompanying drawings:

[0029] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention when not detected.

[0030] Figure 2 For the present invention Figure 1 A is an enlarged structural diagram of FIG.

[0031] Figure 3 It is a schematic diagram of the three-dimensional structure when the detection of the present invention is completed.

[0032] Figure 4 For the present invention Figure 3 Schematic diagram of the enlarged structure at point B.

[0033] Figure 5It is a schematic diagram of the exploded structure of the integral parts of the present invention.

[0034] Figure 6 It is a schematic structural diagram of the active clamping plate and striking assembly of the present invention.

[0035] Figure 7 It is a schematic diagram of the cross-sectional structure of the reaction cup of the present invention when it is separated from the clamping frame in the plate.

[0036] Figure 8 It is a schematic diagram of the guide groove structure of the present invention.

[0037] Figure 9 It is a schematic diagram of the overall side cross-sectional structure of the present invention.

[0038] Figure 10 It is a schematic diagram of the three-dimensional structure of the L-shaped rod and bridge frame of the present invention.

[0039] Figure 11 Schematic diagram of the movement of the striking assembly of the present invention.

[0040] Figure 12 It is a schematic diagram of the L-shaped rod motion structure of the present invention.

[0041] Explanation of reference numerals: 10, reaction cup; 110, reaction tray; 120, enclosure; 130, hollow portion; 140, clamping frame; 20, housing; 210, hydraulic cylinder; 220, active clamping plate; 230, fixed sleeve; 240, elastic sleeve; 260, synchronization rod; 270, output wheel shaft; 280, shaft end wheel; 290, reversing rod; 310, reinforcement seat; 320, striking plate; 330, guide groove; 331, horizontal groove; 332, arc groove; 410, striking block; 420, connecting block; 430, double gear with distance increase; 440, fixed rack; 4 50. Double-tooth rack; 460. Transmission gear; 470. Output rack; 480. Return spring; 490. Piston plate; 510. Reverse plate; 520. L-shaped rod; 530. Horizontal rack; 540. Pressure spring; 550. L-shaped groove; 560. Drive rod; 570. Gear roller; 580. Pulley assembly; 590. Unloading shaft; 610. Detection clamping plate; 611. Docking notch; 620. Cylinder; 630. Fixed frame; 640. Car frame; 641. Corresponding notch; 650. Unloading frame; 710. Piston cylinder; 720. Return air pipe; 730. Exhaust pipe. DETAILED DESCRIPTION

[0042] To make the above-mentioned objects, features, and advantages of the present disclosure more clearly understood, specific embodiments of the present disclosure are described in detail below with reference to the accompanying drawings. The following description sets forth many specific details to facilitate a full understanding of the present disclosure. However, the present disclosure can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without violating the scope of the present disclosure. Therefore, the present disclosure is not limited to the specific embodiments disclosed below.

[0043] In the description of the present disclosure, it is necessary to understand that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present disclosure and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present disclosure. The terms "first" and "second" are used to distinguish one element from another and do not have sequentiality or importance. In addition, when the following description refers to the drawings, the same figure marks in different drawings represent the same or similar elements, which are not repeated in this disclosure.

[0044] In this disclosure, unless otherwise expressly specified or limited, terms such as "installed," "connected," "connect," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components, unless otherwise expressly limited. Those skilled in the art will understand the specific meanings of the above terms in this disclosure based on specific circumstances.

[0045] According to some embodiments of the present disclosure, an immune cell detector and a detection method thereof are provided, referring to Figures 1-12As shown in the figure, the immune cell detector includes a reaction disk 110, a plurality of clamping racks 140 are arranged in a circumferential array on the reaction disk 110, a reaction cup 10 is placed in the clamping rack 140, a panel 120 is coaxially mounted on the outside of the reaction disk 110, a cylinder 620 is provided in the detection direction of the panel 120, a detection clamping plate 610 is fixedly mounted on the telescopic end of the cylinder 620, a car frame 640 is rotatably mounted on the panel 120, and the detection clamping plate 610 and the car frame 640 are intermittently engaged, and an organic shell is provided in the center of the reaction disk 110. 20. A hydraulic cylinder 210 is installed on the housing 20. An active clamping plate 220 is provided at the telescopic end of the hydraulic cylinder 210. The active clamping plate 220 is corresponding to the detection clamping plate 610. A striking plate 320 is rotatably provided on the housing 20. A striking assembly is also provided on the housing 20. When the active clamping plate 220 and the detection clamping plate 610 clamp the reaction cup 10 until the detection is completed, the striking plate 320 drives the cylinder 620 to operate through the striking assembly to realize the separation of the detection clamping plate 610 from the active clamping plate 220.

[0046] Furthermore, a plurality of hollow sections 130 are formed in a circumferential array around the reaction disk 110. A clamping frame 140 is hingedly connected within each of these hollow sections. Clamping frames 140 are configured as curved grippers with silicone tips. A torsion spring is installed between clamping frames 140 and the reaction disk 110 to facilitate repositioning of clamping frames 140.

[0047] Furthermore, a fixed sleeve 230 is installed on the side of the active clamping plate 220, and an elastic sleeve 240 is fixedly installed on the telescopic end of the hydraulic cylinder 210. The elastic sleeve 240 is plugged and engaged with the fixed sleeve 230, and the elastic sleeve 240 is configured as a sleeve with a spring inside.

[0048] A synchronization rod 260 is mounted on the side of the fixed sleeve 230. A reinforcement base 310 is mounted on the side of the housing 20. A striking plate 320 is hingedly mounted on the reinforcement base 310. The striking plate 320 has a guide slot 330 formed therein, through which the synchronization rod 260 passes. A torsion spring is installed at the hinge between the reinforcement base 310 and the striking plate 320 to facilitate the automatic reset of the striking plate 320.

[0049] See Figure 8 The guide groove 330 includes a horizontal groove 331 and an arcuate groove 332, and the arcuate groove 332 is connected to the side of the horizontal groove 331 adjacent to the reaction cup 10; in normal state, the synchronization rod 260 is located in the horizontal groove 331; during detection, the synchronization rod 260 is located in the arcuate groove 332.

[0050] In this way, through the cooperation of the fixed sleeve 230 and the elastic sleeve 240, when the hydraulic cylinder 210 is in operation, the active clamping plate 220 moves toward the reaction cup 10 and contacts it. The hydraulic cylinder 210 continues to extend, and the active clamping plate 220 pushes the reaction cup 10 toward the detection clamping plate 610, so that the reaction cup 10 is gradually clamped by the active clamping plate 220 and the detection clamping plate 610. In this process, the reaction cup 10 gradually breaks away from the clamping of the clamping frame 140. When the active clamping plate 220 and the detection clamping plate 610 completely clamp the reaction cup 10, the reaction cup 10 is already in the detection area and can be detected. At the same time, the hydraulic cylinder 210 continues to operate, and the extension amount is absorbed by the elastic sleeve 240. Due to the setting of the synchronization rod 260, during the operation of the hydraulic cylinder 210, the synchronization rod 260 moves in the guide groove 330, thereby driving the striking plate 320 to rotate, thereby triggering the striking assembly.

[0051] Specifically, a piston cylinder 710 is installed on the casing 20, and a return air pipe 720 and an exhaust pipe 730 are connected to the side of the piston cylinder 710. The return air pipe 720 and the exhaust pipe 730 are connected to the cylinder 620, and the striking plate 320 is intermittently connected to the piston cylinder 710.

[0052] See Figure 6 The striking assembly includes a fixed rack 440 fixedly mounted on the casing 20 and a struck block 410 slidably mounted in the casing 20, and a piston plate 490 performing work in the piston cylinder 710. A double-tooth rack 450 is slidingly arranged on the side of the fixed rack 440, a connecting block 420 is mounted on the side of the striking block 410, and a double-tooth gear 430 with an increased distance is rotatably mounted on the side of the connecting block 420. The double-tooth gear 430 is engaged with the double-tooth rack 450 and the fixed rack 440 at the same time. An output rack 470 is mounted on the side of the piston plate 490, and a transmission gear 460 is rotatably mounted inside the casing 20. The transmission gear 460 is engaged with the output rack 470 and the double-tooth rack 450 at the same time. A reset spring 480 for reset is installed between the striking block 410 and the casing 20.

[0053] When the impact block 410 is moved by an external force, the double-toothed rack 450 moves along the fixed rack 440 through the transmission of the connecting block 420 and the double-toothed gear 430. Due to the rolling travel of the double-toothed gear 430, the actual movement of the double-toothed rack 450 is greater than the movement of the impact block 410. This amplified movement is transmitted to the output rack 470 via the transmission gear 460, which in turn drives the piston plate 490.

[0054] Through the above technical solution, when the immune cell detector provided by the present disclosure is in use, the transmission of the striking assembly is used to trigger the piston cylinder 710 and the cylinder 620, so that when the detection is completed, the synchronization rod 260 is located at the arc peak of the arc-shaped through groove 332. At this time, the striking assembly performs the maximum work, so that the detection clamping plate 610 is driven upward by the cylinder 620, thereby realizing the separation of the detection clamping plate 610 from the active clamping plate 220. At this time, the detection has been completed, the reaction cup 10 loses the support of the detection clamping plate 610, and the spring in the elastic sleeve 240 is released, so that the reaction cup 10 is pushed down by the active clamping plate 220, realizing automatic unloading;

[0055] The cylinder 620 is triggered to move only when the synchronization rod 260 reaches the arc peak of the arc-shaped groove 332, ensuring the absolute stability of the clamping state during the detection process and avoiding detection interruption caused by premature separation;

[0056] This solution can detect different stages through the cooperation of the synchronization rod 260, the striking plate 320, and the guide groove 330, and automatically realize the transfer, clamping, detection in a stable state, and automatic unloading of the reaction cup 10, making the detection more automated, ensuring the detection accuracy, and improving the efficiency of immune cell detection.

[0057] It should be noted that this embodiment also proposes an immune cell detection method, which uses the above-mentioned immune cell detection instrument and includes the following specific steps:

[0058] S1. Sample Collection and Pretreatment: Collect biological samples and pre-treat them by filtering them through a 5-10 micron pore size filter to remove impurities and large cell aggregates. The filtered samples are then centrifuged at 1500-2000 rpm for 5-10 minutes to separate the supernatant containing immune cells.

[0059] S2. Immune Cell Labeling: Add specific immune cell markers to the supernatant, including fluorescein-labeled antibodies and magnetic bead-labeled antibodies. Incubate the supernatant at 37°C for 15-30 minutes to allow the markers to fully bind to the immune cells.

[0060] S3. The incubated sample is treated with a magnetic field, causing the magnetic bead-labeled immune cells to be adsorbed on one side of the magnetic field, thereby separating them from other components. The immune cells adsorbed on one side of the magnetic field are then eluted with an eluent, thereby enriching the immune cells.

[0061] S4. The enriched immune cell sample is placed in a reaction cup 10 and placed on a reaction disk 110. The fluorescein-labeled antibodies on the immune cells are stimulated using excitation light of different wavelengths. The fluorescence signals emitted by the immune cells are detected, and the acquired fluorescence signal data is transmitted to a data processing system. The data processing system uses an algorithm model based on deep learning to process and analyze the data, and generates an immune cell analysis and detection report based on the analysis results. The report content includes information such as the type, quantity, proportion, and various characteristic parameters of the immune cells.

[0062] In some embodiments of the present disclosure, reference Figures 1-12 As shown in , a fixing frame 630 is provided in the detection direction of the reaction disk 110 , the cylinder 620 is fixedly mounted on the fixing frame 630 , a blanking frame 650 is fixedly mounted in the blanking direction of the fixing frame 630 , and the blanking frame 650 is intermittently docked with the car frame 640 .

[0063] The output wheel shaft 270 is rotatably mounted on the end of the synchronization rod 260 , and the output wheel shaft 270 is clearance-matched with the horizontal through slot 331 . The end of the output wheel shaft 270 is fixedly mounted with an end wheel 280 , and a return rod 290 is mounted on the side of the end wheel 280 .

[0064] An L-shaped groove 550 is provided on the casing 20 , in which a reversing plate 510 is slidably installed. The reversing plate 510 is vertically connected to an L-shaped rod 520 . The reversing plate 510 and the reversing rod 290 are intermittently engaged, and the L-shaped rod 520 is transmission-connected to the car frame 640 .

[0065] Among them, a blanking shaft 590 is rotatably mounted on the enclosure 120, the car frame 640 is fixedly mounted on the blanking shaft 590, a driving rod 560 is rotatably mounted on the casing 20, a pulley set 580 is commonly installed between the blanking shaft 590 and the driving rod 560, a gear roller 570 is coaxially mounted on the blanking shaft 590, and a transverse rack 530 is connected to the end of the L-shaped rod 520, which meshes with the gear roller 570;

[0066] When the reversing plate 510 rotates and toggles the reversing rod 290 to move, the car frame 640 rotates and docks with the unloading frame 650 .

[0067] Specifically, a pressure spring 540 is installed between the reversing plate 510 and the wall of the L-shaped groove 550 , a corresponding notch 641 is provided at one end of the car frame 640 adjacent to the detection clamping plate 610 , and a docking notch 611 is provided at the corresponding end of the detection clamping plate 610 .

[0068] It should be noted that the pulley assembly 580 is configured as a conventional combination of a transmission wheel and a belt, the two transmission wheels are respectively mounted on the blanking shaft 590 and the driving rod 560, and the belt is sleeved on the outside of the two transmission wheels.

[0069] Through the above technical solution, when the immune cell detector provided by the present disclosure is in use, the corresponding notch 641 is docked with the docking notch 611 through the cooperation of the carriage frame 640 and the detection clamping plate 610, so that the clamping of the reaction cup 10 is more stable, and when the synchronization rod 260 moves to the arc peak of the arc groove 332, the detection has ended. At this time, the striking assembly is triggered, the detection clamping plate 610 moves up and away from the carriage frame 640, and through the cooperation of the reversing rod 290 and the reversing plate 510, after the synchronization rod 260 passes the arc peak of the arc groove 332, the reversing rod 290 rotates and falls on the reversing plate 510 and drives The reversing plate 510 moves, causing the transverse rack 530 to drive the gear roller 570 to rotate. After a series of transmissions, the rotation of the car frame 640 is realized. Originally, the car frame 640 is horizontal and docked with the detection clamping plate 610. After the detection clamping plate 610 leaves, the car frame 640 rotates and changes to dock with the discharge rack 650. At this time, the reaction cup 10 is pushed by the active clamping plate 220 to slide down along the discharge rack 650 for discharge, and the sliding buffer is provided for the discharge of the reaction cup 10 to avoid damage to the cup body of the reaction cup 10 caused by direct push. The discharge rack 650 is convenient for docking with external automation equipment for other processing.

[0070] The preferred embodiments of the present disclosure are described in detail above in conjunction with the accompanying drawings. However, the present disclosure is not limited to the specific details of the above embodiments. Within the technical concept of the present disclosure, various simple modifications can be made to the technical solutions of the present disclosure, and these simple modifications all fall within the scope of protection of the present disclosure.

[0071] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present disclosure will not further describe various possible combinations.

[0072] In addition, the various embodiments of the present disclosure may be arbitrarily combined, and as long as they do not violate the concept of the present disclosure, they should also be regarded as the contents disclosed by the present disclosure.

Claims

1. An immune cell detector, comprising a reaction disk (110), a plurality of clamping racks (140) arranged in a circumferential array on the reaction disk (110), a reaction cup (10) being placed in the clamping racks (140), and characterized in that: A panel (120) is coaxially mounted on the outside of the reaction disk (110), a cylinder (620) is provided in the detection direction of the panel (120), a detection clamping plate (610) is fixedly mounted on the telescopic end of the cylinder (620), a car frame (640) is rotatably mounted on the panel (120), the detection clamping plate (610) and the car frame (640) are intermittently engaged, a housing (20) is provided at the center of the reaction disk (110), a hydraulic cylinder (210) is mounted on the housing (20), and a hydraulic cylinder (210) is provided on the telescopic end of the hydraulic cylinder (210). An active clamping plate (220), the active clamping plate (220) and the detection clamping plate (610) are correspondingly arranged, a striking plate (320) is rotatably arranged on the housing (20), and a striking assembly is further arranged on the housing (20), and the active clamping plate (220) and the detection clamping plate (610) clamp the reaction cup (10) until the detection is completed, and the striking plate (320) drives the cylinder (620) to operate through the striking assembly, thereby realizing the separation of the detection clamping plate (610) and the active clamping plate (220); A fixed sleeve (230) is installed on the side of the active clamping plate (220), and an elastic sleeve (240) is fixedly installed on the telescopic end of the hydraulic cylinder (210). The elastic sleeve (240) is plug-engaged with the fixed sleeve (230), and the elastic sleeve (240) is configured as a sleeve with a spring inside. A synchronization rod (260) is installed on the side of the fixed sleeve (230), a reinforcement seat (310) is installed on the side of the housing (20), the striking plate (320) is hingedly mounted on the reinforcement seat (310), a guide slot (330) is provided on the striking plate (320), and the synchronization rod (260) passes through the guide slot (330); The guide through groove (330) includes a horizontal through groove (331) and an arcuate through groove (332), wherein the arcuate through groove (332) is connected to a side of the horizontal through groove (331) adjacent to the reaction cup (10); In normal state, the synchronization rod (260) is located in the horizontal through groove (331); During detection, the synchronization rod (260) is located in the arc-shaped through groove (332); A piston cylinder (710) is mounted on the housing (20), a return air pipe (720) and an exhaust pipe (730) are connected to the side of the piston cylinder (710), the return air pipe (720) and the exhaust pipe (730) are connected to the cylinder (620), and the striking plate (320) is intermittently connected to the piston cylinder (710); The striking assembly comprises a fixed rack (440) fixedly mounted on the housing (20), a striking block (410) slidably mounted in the housing (20), and a piston plate (490) working in the piston cylinder (710), a double-tooth rack (450) being slidably mounted on the side of the fixed rack (440), a connecting block (420) being mounted on the side of the striking block (410), a double-gear (430) being rotatably mounted on the side of the connecting block (420), and the adding The double gear (430) is meshed with the double-tooth rack (450) and the fixed rack (440) at the same time, an output rack (470) is installed on the side of the piston plate (490), a transmission gear (460) is rotatably installed inside the housing (20), and the transmission gear (460) is meshed with the output rack (470) and the double-tooth rack (450) at the same time, and a reset spring (480) for reset is installed between the impact block (410) and the housing (20).

2. The immune cell detector according to claim 1, characterized in that: A plurality of hollow portions (130) are formed in a circumferential array on the reaction disk (110), and the clamping frame (140) is hinged in the hollow portions (130). The clamping frame (140) is configured as an arc-shaped clamping hand with a silicone head as a clamping end.

3. The immune cell detector according to claim 1, characterized in that: A fixing frame (630) is provided in the detection direction of the reaction disk (110), the cylinder (620) is fixedly mounted on the fixing frame (630), a material unloading frame (650) is fixedly mounted in the unloading direction of the fixing frame (630), and the material unloading frame (650) is intermittently docked with the car frame (640); An output wheel shaft (270) is rotatably mounted on the end of the synchronization rod (260), the output wheel shaft (270) is clearance-matched with the horizontal through slot (331), an axis end wheel (280) is fixedly mounted on the end of the output wheel shaft (270), and a reversing rod (290) is mounted on the side of the axis end wheel (280); An L-shaped groove (550) is provided on the housing (20), a reversing plate (510) is slidably mounted in the L-shaped groove (550), the reversing plate (510) is vertically connected to an L-shaped rod (520), the reversing plate (510) and the reversing rod (290) are intermittently engaged, and the L-shaped rod (520) is transmission-connected to the car frame (640).

4. The immune cell detector according to claim 3, characterized in that: A blanking shaft (590) is rotatably mounted on the enclosure (120), the car frame (640) is fixedly mounted on the blanking shaft (590), a driving rod (560) is rotatably mounted on the housing (20), a pulley set (580) is commonly mounted between the blanking shaft (590) and the driving rod (560), a gear roller (570) is coaxially mounted on the blanking shaft (590), a transverse rack (530) is connected to the end of the L-shaped rod (520), and the transverse rack (530) is meshed with the gear roller (570); When the reversing plate (510) rotates and drives the reversing rod (290) to move, the car frame (640) rotates and docks with the unloading frame (650).

5. The immune cell detector according to claim 4, characterized in that: A pressure spring (540) is installed between the reversing plate (510) and the wall of the L-shaped groove (550), a corresponding notch (641) is provided at one end of the car frame (640) adjacent to the detection clamping plate (610), and a docking notch (611) is provided at the corresponding end of the detection clamping plate (610).

6. A method for detecting immune cells, characterized in that: Using the immune cell detector according to any one of claims 1 to 5 comprises the following specific steps: S1. Sample Collection and Pretreatment: Collect biological samples and pre-treat them by filtering them through a 5-10 micron pore size filter to remove impurities and large cell aggregates. The filtered samples are then centrifuged at 1500-2000 rpm for 5-10 minutes to separate the supernatant containing immune cells. S2. Immune cell labeling: Add specific immune cell markers to the supernatant, including fluorescein-labeled antibodies and magnetic bead-labeled antibodies. Incubate the supernatant with the markers at 37°C for 15-30 minutes to allow the markers to fully bind to the immune cells. S3. The incubated sample is treated with a magnetic field, causing the magnetic bead-labeled immune cells to be adsorbed on one side of the magnetic field, thereby separating them from other components. The immune cells adsorbed on one side of the magnetic field are then eluted with an eluent, thereby enriching the immune cells. S4. The enriched immune cell sample is placed in a reaction cup (10) and placed on a reaction plate (110). The fluorescein-labeled antibodies on the immune cells are stimulated using excitation light of different wavelengths. The fluorescence signals emitted by the immune cells are detected. The acquired fluorescence signal data is transmitted to a data processing system. The data processing system uses an algorithm model based on deep learning to process and analyze the data. An immune cell analysis and detection report is generated based on the analysis results. The report content includes information such as the type, number, proportion, and various characteristic parameters of the immune cells.

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