Premna microphylla polysaccharide composite gel for promoting healing of diabetic wounds and preparation method of premna microphylla polysaccharide composite gel

Abstract

The invention discloses premna microphylla polysaccharide composite gel for promoting healing of diabetic wounds. The premna microphylla polysaccharide composite gel comprises attapulgite, premna microphylla polysaccharide and chitosan, the mass ratio of the attapulgite to the premna microphylla polysaccharide to the chitosan is 1: (0.4-12): (0.1-2). The invention also discloses a preparation method of the premna microphylla polysaccharide composite gel. The composite gel serving as the wound dressing has good mechanical performance, biocompatibility and multi-dimensional biological activity and is especially suitable for healing of diabetic wounds, the preparation process is green, operation is easy, and raw materials are low in cost.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical materials for promoting wound healing, and specifically relates to a composite gel for promoting wound healing, and more specifically to a composite gel of tofu bud polysaccharide for promoting diabetic wound healing and a preparation method thereof. Background Art

[0002] Premna microphylla Turcz., also known as tofu wood, is a plant of the Verbenaceae family. It is cold in nature, bitter, and slightly pungent in taste, and enters the liver and large intestine meridians, making it highly valuable for medicinal, edible, and economic use. According to the Hunan Materia Medica and the Annotated Compendium of Materia Medica, Premna microphylla Turcz. has the efficacy of clearing heat and detoxifying, and is primarily used to treat malaria, diarrhea, dysentery, headaches caused by alcohol consumption, and hemorrhage caused by trauma. The edible and economic value of Premna microphylla is reflected in the smooth texture and excellent flavor of the processed Premna microphylla jelly, which is a purely natural edible product and contains no preservatives. Premna microphylla polysaccharides, the primary active ingredient in Premna microphylla, play a significant role in its food and pharmaceutical applications.

[0003] To date, research on tofu taro has mainly focused on food development, with tofu taro leaves being used to develop a variety of foods such as "magic tofu", beverages, jellies, and vermicelli. Chinese patent CN201811065827.5 discloses a method for preparing tofu taro pectin jelly powder by mixing tofu taro leaf dry powder, tofu taro leaf low-ester pectin powder, calcium carbonate, and citric acid. Chinese patent CN202011142351.8 discloses a tofu taro juice lactic acid bacteria beverage made from tofu taro leaves, which is alcohol-free, low in sugar, and low in sodium, and has the effects of anti-inflammatory, sterilization, prevention of intestinal infectious diseases, and prevention of cardiovascular and cerebrovascular diseases. However, there are currently few reports on the research on tofu taro in biomedical applications.

[0004] Hydrogels are highly water-retaining materials with a three-dimensional network structure, formed by the interaction of polymers or colloidal particles, with water as the dispersion medium. The high water content of biocompatible hydrogels can provide a moist environment suitable for wound cell healing. Therefore, combining bioactive tofu shiitake mushroom polysaccharides with gel material science could broaden the potential for high-value applications of tofu shiitake mushroom polysaccharides in the biomedical field.

[0005] Delayed wound healing in diabetic patients is characterized by complex mechanisms and long treatment cycles, placing a heavy burden on patients' lives and socioeconomic well-being. Wound healing is a complex and dynamic biological process encompassing four phases: hemostasis, inflammation, proliferation, and remodeling. Each phase progresses smoothly through the collaborative efforts of multiple cells. Diabetic wounds, due to abnormal blood glucose levels, present with imbalanced inflammatory responses, oxidative stress, inadequate angiogenesis, and a high risk of bacterial infection. This leads to increased production of reactive oxygen species (ROS) by macrophages to defend against foreign pathogens. However, excessive ROS not only damages normal cells and tissues but can also cause angiogenesis impairment, nerve damage, hypoxia, and nutrient deficiency, disrupting all stages of wound healing and ultimately leading to persistent inflammation and chronic wounds. Currently, traditional wound dressings have limitations in the treatment of diabetic wounds, making them ineffective in addressing the complex pathological environment of diabetic wounds, such as regulating local hyperglycemia, controlling inflammation, and promoting angiogenesis. The application of natural polysaccharides in diabetic wound gels offers a promising approach to address these challenges. Summary of the Invention

[0006] The present invention addresses the deficiencies of the above-mentioned prior art and provides a composite gel of tofu shiitake mushroom polysaccharide for promoting diabetic wound healing and a method for preparing the same. The composite gel of the present invention exhibits excellent mechanical properties, biocompatibility, and multidimensional bioactivity as a wound dressing, making it particularly suitable for diabetic wound healing. Furthermore, the preparation process is environmentally friendly, simple to operate, and uses inexpensive raw materials.

[0007] The specific technical solutions are as follows:

[0008] One of the purposes of the present invention is to provide a tamarind polysaccharide composite gel for promoting diabetic wound healing, the components of which include attapulgite, tamarind polysaccharide and chitosan; the mass ratio of attapulgite, tamarind polysaccharide and chitosan is 1: (0.4-12): (0.1-2).

[0009] The mechanism of the present invention is as follows: the electrostatic interaction between the surface carboxyl groups of tamarind polysaccharide and the amino groups of chitosan creates the framework structure of the composite gel, while the one-dimensional rigid nanomaterial of attapulgite imparts excellent mechanical properties to the composite gel. The present invention applies the tamarind polysaccharide composite gel to wound dressings and adopts a reasonable proportion. On the one hand, the hydrogel provides a moist environment suitable for cell growth, and on the other hand, it fully synergizes the anti-inflammatory, antibacterial, and hemostatic properties of tamarind polysaccharide, chitosan, and attapulgite. Compared with existing technologies, it has a more significant effect on the healing of diabetic wounds.

[0010] The tofu shiitake mushroom polysaccharide is obtained from tofu shiitake mushroom as raw material through water extraction and alcohol precipitation. Specifically, the alcohol is ethanol.

[0011] Furthermore, in the tamarind polysaccharide composite gel, the mass ratio of attapulgite, tamarind polysaccharide and chitosan is preferably 1:(0.4-6):(0.1-1).

[0012] Furthermore, the deacetylation degree of chitosan is preferably ≥95%, and the viscosity is preferably 100 to 200 mPa·s.

[0013] A second object of the present invention is to provide a method for preparing the aforementioned tofu bud polysaccharide composite gel, comprising the following steps:

[0014] S1. Obtaining tofu bud polysaccharide;

[0015] S2. The attapulgite was crushed and dispersed in water to prepare an attapulgite slurry; chitosan was dissolved in an aqueous acetic acid solution to prepare chitosan acetic acid solution; the tofu Chai polysaccharide was dissolved in water to configure the tofu Chai polysaccharide solution;

[0016] S3. The tofu Chai polysaccharide solution was blended with the attapulgite slurry and the pH was adjusted to acidic to obtain an attapulgite / tofu Chai polysaccharide mixed system; chitosan acetic acid solution was added to the attapulgite / tofu Chai polysaccharide mixed system and stirred to obtain a composite sol;

[0017] S4. Immerse the composite sol in water to obtain a composite gel of tofu bud polysaccharide.

[0018] Furthermore, in step S1: water is added to the tofu-chai raw material for extraction, and the extract is subjected to ethanol precipitation to obtain the precipitate.

[0019] Specifically, the tofu bud polysaccharide is prepared by the following method: the tofu bud leaves are dried and then crushed to obtain tofu bud powder; water is added to the tofu bud powder for reflux extraction to obtain an extract; ethanol is added to the extract and allowed to stand for 12 to 48 hours, and the precipitate is dried to obtain the product.

[0020] The reflux extraction temperature is preferably 60 to 90° C., and the reflux extraction time is preferably 3 to 6 hours.

[0021] In the reflux extraction, the solid-liquid ratio of tofu chai powder to water is preferably 1 g: (5-20) mL.

[0022] Wherein, the ethanol is preferably anhydrous ethanol, and preferably 3 to 10 times the volume of anhydrous ethanol is added to the extract.

[0023] Wherein, before reflux extraction, the tofu chai powder is preferably subjected to ethanol reflux for impurity removal.

[0024] Furthermore, in step S2: the concentration of the attapulgite slurry is preferably 1 wt% to 5 wt%.

[0025] Furthermore, in step S2: the concentration of chitosan in the chitosan acetate solution is preferably 1 wt% to 2 wt%.

[0026] Furthermore, in step S2: the concentration of the acetic acid aqueous solution is preferably 1 wt% to 2 wt%.

[0027] Furthermore, in step S2: the concentration of the tofu bud polysaccharide solution is preferably 2wt% to 12wt%.

[0028] Furthermore, in step S3: the pH is preferably adjusted to 1.0-2.0.

[0029] Furthermore, in step S4, the immersion time is preferably 24 to 72 hours, and the immersion water is preferably replaced every 6 to 12 hours during the immersion period.

[0030] Furthermore, in step S4, the volume of the immersion water is at least 8 times, more preferably 8 to 20 times, the volume of the composite sol, so that the composite sol can be fully gelled.

[0031] Furthermore, in the above steps, the water is preferably deionized water.

[0032] The third object of the present invention is to provide a wound dressing for promoting the healing of diabetic wounds, which comprises the above-mentioned Fucha polysaccharide composite gel. Specifically, the wound dressing may further comprise auxiliary materials.

[0033] The beneficial effects of the present invention are as follows:

[0034] (1) A composite gel wound dressing was prepared using attapulgite, tofu sorghum polysaccharide, and chitosan as raw materials. It can be used to promote the healing of diabetic wounds and broaden the application prospects of tofu sorghum in the field of biomedicine.

[0035] (2) Tofu bud polysaccharide has good anti-inflammatory and hemostatic biological activities, chitosan has good antibacterial activity, and attapulgite has procoagulant activity, all of which provide a material active basis for promoting the healing of diabetic wounds.

[0036] (3) The electrostatic interaction between the surface carboxyl groups of tofu bud polysaccharide and the amino groups of chitosan constructed the framework structure of the composite gel, and the one-dimensional rigid nanomaterial of attapulgite gave the composite gel good mechanical properties.

[0037] (4) The composite gel of tofu bud polysaccharide is made of tofu bud polysaccharide of traditional Chinese medicine as the main material, and chitosan and attapulgite as the auxiliary materials. The raw materials are widely available, clean and easy to obtain, and the preparation process is simple and green and non-toxic. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is the adhesion image of the tofu bud polysaccharide composite gel in Example 1;

[0039] Figure 2 This is the scanning electron microscope (SEM) image of the Dauci fuciformis polysaccharide composite gel in test 1;

[0040] Figure 3 This is the rheological characteristic curve of the tofu bud polysaccharide composite gel in test 1 (the horizontal axis of the left figure is the oscillation strain, and the horizontal axis of the right figure is the angular frequency);

[0041] Figure 4 This is the compressive stress-strain characteristic curve of the tofu bud polysaccharide composite gel in test 1 (in the figure: the horizontal axis is the compressive strain, and the vertical axis is the compressive stress);

[0042] Figure 5 To test the coagulation properties of the tofu bud polysaccharide composite gel in Example 1 and the chitosan-attapulgite composite in Comparative Example 1;

[0043] Figure 6 Photos of the blood-absorbing filter paper of the tofu stalk polysaccharide composite gel of Example 1 and the chitosan-attapulgite composite of Comparative Example 1 in Test 2;

[0044] Figure 7 In Test 2, the blood absorption capacity of the tofu taro polysaccharide composite gel of Example 1 and the chitosan-attapulgite composite of Comparative Example 1 was compared (in the figure: the vertical axis is the blood absorption capacity);

[0045] Figure 8 Microscopic images of cell migration of the tofu bud polysaccharide composite gel of Example 1 and the chitosan-attapulgite composite of Comparative Example 1 in Test 2;

[0046] Figure 9 The cell migration rate of the tofu taro polysaccharide composite gel of Example 1 and the chitosan-attapulgite composite of Comparative Example 1 in Test 2 was compared (in the figure: the vertical axis is the cell migration rate);

[0047] Figure 10 This is to compare the performance of the tofu bud polysaccharide composite gel of Example 1 and the chitosan-attapulgite composite of Comparative Example 1 in promoting diabetic wound healing (in the figure: the horizontal and vertical axes are both wound lengths). DETAILED DESCRIPTION

[0048] The principles and features of the present invention are described below with reference to examples. The examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial sources.

[0049] In a specific embodiment, the chitosan used has a deacetylation degree of ≥95% and a viscosity of 100-200 mPa·s.

[0050] Example 1

[0051] The steps for preparing a tofu bud polysaccharide composite gel for promoting diabetic wound healing are as follows:

[0052] S1. Preparation of tofu bud polysaccharide: Take fresh tofu bud leaves, dry them, grind them into powder and crush them to obtain tofu bud powder; add anhydrous ethanol to the tofu bud powder and reflux to remove impurities, centrifuge and precipitate, add deionized water according to the solid-liquid ratio of tofu bud powder to water of 1g:10mL, reflux and extract at 80℃ for 3h; add 5 times the volume of anhydrous ethanol to the extract and let it stand for 24h, take the precipitate and freeze-dry to obtain tofu bud polysaccharide.

[0053] S2. Take 1.0g of attapulgite and crush it and disperse it in 20mL of deionized water. Prepare 4.8wt% attapulgite slurry by beating at 10000rpm; take 0.15g of chitosan and dissolve it in 10mL of 2wt% acetic acid aqueous solution and stir until dissolved to obtain a chitosan acetic acid solution with a chitosan concentration of 1.5wt%; take 0.6g of tofu bud polysaccharide obtained in step S1 and dissolve it in 10mL of deionized water to obtain a 5.7wt% tofu bud polysaccharide solution.

[0054] S3. The attapulgite slurry obtained in step S2 is added to the tofu sapogenin polysaccharide solution and stirred evenly, and the pH of the mixed system is adjusted to 1.0 using hydrochloric acid; a attapulgite / tofu sapogenin polysaccharide mixed system is obtained; the chitosan acetic acid solution obtained in step S2 is added to the attapulgite / tofu sapogenin polysaccharide mixed system and stirred evenly to obtain a composite sol.

[0055] S4. The composite sol obtained in step S3 was immersed in 400 mL of deionized water for 72 h, and the deionized water was changed every 12 h to fully gelate to obtain a composite gel of tofu taro polysaccharide. The obtained composite gel of tofu taro polysaccharide had good adhesion, as shown in the photo of the adhesion. Figure 1 .like Figure 1 As shown in the results, the tofu bud polysaccharide gel can stably adhere to glass, plastic, paper, tinfoil and skin.

[0056] Example 2

[0057] Referring to Example 1, the difference from Example 1 is that,

[0058] In step S2: 1.0 g of attapulgite is crushed and dispersed in 20 mL of deionized water, and 4.8 wt% of attapulgite slurry is prepared by high-speed beating at 10,000 rpm; 0.3 g of chitosan is dissolved in 15 mL of 2 wt% acetic acid aqueous solution and stirred until dissolved to obtain a chitosan acetic acid solution; 1.2 g of the tofu bud polysaccharide obtained in step S1 is dissolved in 15 mL of deionized water to obtain a tofu bud polysaccharide solution.

[0059] In step S4: the composite sol obtained in step S3 is immersed in 500 mL of deionized water for 72 hours, and the deionized water is replaced every 12 hours to fully gelate to obtain a composite gel of tofu bud polysaccharide.

[0060] Example 3

[0061] Referring to Example 1, the difference from Example 1 is that,

[0062] In step S2: 1.0 g of attapulgite is crushed and dispersed in 20 mL of deionized water, and 4.8 wt% attapulgite slurry is prepared by high-speed beating at 10,000 rpm; 0.6 g of chitosan is dissolved in 30 mL of 2 wt% acetic acid aqueous solution and stirred until dissolved to obtain a chitosan acetic acid solution; 4 g of the tofu bud polysaccharide obtained in step S1 is dissolved in 40 mL of deionized water to obtain a tofu bud polysaccharide solution.

[0063] In step S4: the composite sol obtained in step S3 is immersed in 900 mL of deionized water for 72 hours, and the deionized water is replaced every 12 hours to fully gelate to obtain a composite gel of tofu bud polysaccharide.

[0064] Comparative Example 1

[0065] The chitosan-attapulgite composite was prepared by referring to Example 1, with the following differences from Example 1:

[0066] No tofu bud polysaccharide solution was prepared;

[0067] In step S3: the attapulgite slurry is added to 10 mL of deionized water and stirred evenly, and the pH of the mixed system is adjusted to 1.0 with hydrochloric acid; chitosan acetate solution is continuously added thereto and stirred evenly to obtain a composite solution; and chitosan-attapulgite composite is obtained by immersion deacidification in step S4.

[0068] The remaining technical features are the same as those in Example 1.

[0069] Test 1 Physical and chemical properties test

[0070] 1. The microstructure of the tofu bud polysaccharide composite gel was observed using a scanning electron microscope. The tofu bud polysaccharide composite gel obtained in Example 1 was rapidly frozen at -195°C for 10 minutes and then freeze-dried to obtain the tofu bud polysaccharide composite aerogel. A cross section of the tofu bud polysaccharide composite aerogel was adhered to a conductive adhesive, and gold was plated on the sample surface using a Quorum SC7620 sputter coater. The microstructure of the tofu bud polysaccharide composite gel was observed using a SOLARIS GMH focused ion electron dual-beam scanning electron microscope at ×2k and ×5k magnifications. The scanning electron microscope image of the tofu bud polysaccharide composite gel is shown in Figure 2 ;like Figure 2 As shown, the tofu-tree polysaccharide composite gel exhibits a three-dimensional porous network structure.

[0071] 2. The dynamic rheological properties of the composite gel of tofu bud polysaccharide obtained in Example 1 were evaluated using an ARES-G2 rheometer. First, the linear viscoelastic region of the gel was determined by an amplitude sweep test. The gel was loaded onto the test plate of the rheometer to cause an oscillatory shear deformation with an angular frequency of 10 rad / s and a stress range of 0.1% to 100%. After the amplitude sweep, a frequency sweep test was performed at a constant strain of 1% within the frequency sweep range of 0.1 to 100 rad / s. The rheological properties of the composite gel of tofu bud polysaccharide obtained in Example 1 are as follows: Figure 3 As shown in the figure, the storage modulus G' is greater than the loss modulus G", which further verifies the successful synthesis of the tofu bud polysaccharide composite gel.

[0072] 3. Compression testing was conducted using a 68TM-5 universal testing machine equipped with a 100N load cell to evaluate the mechanical strength of the sugi-like polysaccharide composite gel obtained in Example 1. A 25 mm diameter, 5 mm thick sugi-like polysaccharide composite gel was placed on a test plate. Under conditions of a compression rate of 1 mm / min and a strain range of 30%, a compressive stress-strain curve for the sugi-like polysaccharide composite gel in Example 1 was obtained, as shown in Figure 4. The elastic modulus of the sugi-like polysaccharide composite gel in Example 1 was calculated to be 278.73 ± 4.61 Pa.

[0073] Test 2 In vitro bioactivity analysis

[0074] 1. Blood coagulation index (BCI) analysis. The blood coagulation index is often used to evaluate the ability of hydrogels to promote blood coagulation. Take anticoagulated whole blood from mice and slowly add it drop by drop to the surface of the tofu taro polysaccharide composite gel or chitosan-attapulgite composite. Immediately afterwards, 10 μL of 0.2M CaCl2 solution was added to the blood sample. After incubation at 37°C for 10 minutes, the tofu taro polysaccharide composite gel or chitosan-attapulgite composite was immersed in deionized water, and the absorbance of the solution at 540 nm was measured. The absorbance value of whole blood in deionized water was used as a reference. The BCI value of the tofu taro polysaccharide composite gel and the chitosan-attapulgite composite was calculated by the following formula:

[0075] BCI (%) = As / Ar × 100%;

[0076] Among them, As and Ar are the absorbance values of the experimental group (Dauci fructus sylvestris polysaccharide composite gel or chitosan-attapulgite composite) and the reference group, respectively.

[0077] The test results of each embodiment and comparative example are shown in Table 1 and Figure 5 ; Figure 5 Middle: The control group is the chitosan-attapulgite composite in Comparative Example 1, and the composite gel group is the tofu bud polysaccharide composite gel in Example 1. Figure 5As shown in Table 1, the BCI value of the Dauci radiata polysaccharide composite gel obtained in Example 1 is 27.44±2.04%, indicating that the Dauci radiata polysaccharide composite gel has good procoagulant ability, which is much better than that of Comparative Example 1.

[0078] Table 1 Blood coagulation index (BCI) of Examples and Comparative Examples

[0079] Group Example 1 Comparative Example 1 Example 2 Example 3 BCI value 27.44±2.04％ 41.06±3.16％ 24.83±4.63％ 23.57±3.74％

[0080] 2. Evaluate the hemostatic ability of the Tofu Chai polysaccharide composite gel. Take fresh mouse liver, use a 20G needle to induce liver bleeding in the mouse, and immediately apply the Tofu Chai polysaccharide composite gel in Example 1 or the chitosan-attapulgite complex in Comparative Example 1 to the wound surface. The blank group is not treated. Weigh and record the weight of the blood-absorbing filter paper, and calculate the blood-absorbing weight. Figure 6 As shown, the blood sucking weight is as Figure 7 shown. Figure 6 、 Figure 7 Middle: The control group is the chitosan-attapulgite complex in Comparative Example 1, and the composite gel group is the tofu bud polysaccharide composite gel in Example 1.

[0081] See also Figure 6 、 Figure 7 Compared with the blank group and the control group, the bleeding amount of the wound treated with the tofu bud polysaccharide composite gel in Example 1 was significantly reduced (28.63±1.67 mg), showing good hemostatic performance.

[0082] 3. Using mouse fibroblasts (L929) as a model, the cell migration ability of the tofu bud polysaccharide composite gel was evaluated. L929 cells were inoculated in a 12-well plate and incubated for 24 hours, and then the bottom of each well was vertically scratched with the tip of a plastic pipette. Cell debris was removed by washing 3 times with PBS. The culture medium was replaced with 200 μL of hydrogel (Example 1, Comparative Example 1) extract and incubated for 12 and 24 hours. Cells cultured in a medium without FBS were set as a blank group. Images were acquired for a certain period of time using a microscope to record the migration of cells to the scratched area, such as Figure 8 The cell migration rate is shown as Figure 9 shown. Figure 8 、 Figure 9 Middle: The control group is the chitosan-attapulgite complex in Comparative Example 1, and the composite gel group is the tofu bud polysaccharide composite gel in Example 1.

[0083] See also Figure 8 、 Figure 9At 12h and 24h, the migration rates of cells treated with the tofu bud polysaccharide composite gel of Example 1 were 37.15±1.80% and 47.34±1.52%, respectively, which were significantly better than those of the blank group and the control group, indicating that the tofu bud polysaccharide composite gel promoted cell migration and was beneficial to diabetic wound healing.

[0084] Test 3: In vivo promotion of diabetic wound healing test

[0085] In order to evaluate the effect of Tofu-soybean polysaccharide composite gel on the healing of diabetic wounds, a diabetic mouse skin wound model was established with the help of C57 / BL6 male mice. After one week of adaptive feeding, C57 / BL6 male mice were continuously intraperitoneally injected with streptozotocin (50 mg / kg) to induce elevated blood sugar. Subsequently, diabetic mice with blood sugar values greater than 16.6 mM were randomly divided into a blank group, a control group (Chitosan-attapulgite complex of Comparative Example 1), and a composite gel group (Tofu-soybean polysaccharide composite gel of Example 1). After the mice were anesthetized, the backs were shaved and disinfected, and then a circular skin wound with a diameter of 10 mm was made. Wound treatment: Medical sterile gauze was used in the blank group, the control group used the chitosan-attapulgite complex of Comparative Example 1, and the composite gel group used the Tofu-soybean polysaccharide composite gel of Example 1. Gently wipe the wound with sterile saline regularly, and remove the dressing to avoid secondary damage. Wound images were acquired with a camera on days 0, 3, 7, and 14, and the wound area was calculated using image J software, as shown in the following figure: Figure 10 As shown. Figure 10 As can be seen, the Dangsuan polysaccharide composite gel of the present invention significantly outperformed both the blank and control groups in terms of diabetic wound healing. The control group consisted of a chitosan-attapulgite composite. Although chitosan is a recognized effective gel dressing material, its gel properties and healing effects on diabetic wounds were not ideal. The Dangsuan polysaccharide composite gel of the present invention not only possessed excellent mechanical properties and a stable structure, but also demonstrated significant efficacy as a diabetic wound dressing, effectively promoting diabetic wound healing.

[0086] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A composite gel of tofu bud polysaccharide for promoting diabetic wound healing, characterized in that: The invention comprises attapulgite, taurine polysaccharide and chitosan; the mass ratio of attapulgite, taurine polysaccharide and chitosan is 1:(0.4-12):(0.1-2).

2. The composite gel of tofu bud polysaccharide according to claim 1, characterized in that The tofu bud polysaccharide is obtained from tofu bud as a raw material through a water extraction and alcohol precipitation method.

3. The composite gel of tofu bud polysaccharide according to claim 1 or 2, characterized in that The mass ratio of attapulgite, tofu tung polysaccharide and chitosan is 1:(0.4-6):(0.1-1).

4. A method for preparing the composite gel of tofu bud polysaccharide according to any one of claims 1 to 3, characterized in that: The steps include: S1. Obtaining tofu bud polysaccharide; S2. The attapulgite was crushed and dispersed in water to prepare an attapulgite slurry; chitosan was dissolved in an aqueous acetic acid solution to prepare chitosan acetic acid solution; the tofu Chai polysaccharide was dissolved in water to configure the tofu Chai polysaccharide solution; S3. The tofu Chai polysaccharide solution was blended with the attapulgite slurry and the pH was adjusted to acidic to obtain an attapulgite / tofu Chai polysaccharide mixed system; chitosan acetic acid solution was added to the attapulgite / tofu Chai polysaccharide mixed system and stirred to obtain a composite sol; S4. Immerse the composite sol in water to obtain a composite gel of tofu bud polysaccharide.

5. The preparation method according to claim 4, characterized in that In step S2: The concentration of the attapulgite slurry is 1wt% to 5wt%; The concentration of chitosan in the chitosan acetate solution is 1 wt% to 2 wt%; The concentration of the acetic acid aqueous solution is 1wt% to 2wt%; The concentration of the tofu bud polysaccharide solution is 2wt% to 12wt%.

6. The preparation method according to claim 4, characterized in that In step S3: adjust the pH to 1.0-2.

0.

7. The preparation method according to claim 4, characterized in that In step S4: The soaking time is 24 to 72 hours; The volume of the impregnation water is more than 8 times that of the composite sol.

8. The preparation method according to claim 4, characterized in that In step S1: water is added to the tofu-chai raw material for extraction, and the extract is subjected to ethanol precipitation to obtain the precipitate.

9. The preparation method according to claim 4, characterized in that The water is deionized water.

10. A wound dressing for promoting healing of diabetic wounds, comprising the Dauci bupleuri polysaccharide composite gel according to any one of claims 1 to 3.

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