High-flux thermal sensing platform and use method thereof

Abstract

The invention discloses a high-flux heat sensing platform and a use method thereof. The high-flux heat sensing platform comprises a base, a first heating part, a culture carrying table, a heat insulation cavity body, a heat insulation cavity cover plate, a second heating part, a heating upper cover, a top cover plate, a culture chamber and a thermopile sensor. The base, the first heating part, the culture carrying table, the heat insulation cavity main body, the heat insulation cavity cover plate, the second heating part, the heating upper cover and the top cover plate are sequentially stacked. According to the high-flux heat sensing platform and the using method thereof, real-time monitoring of multi-point temperature is achieved, the temperature of the high-flux heat sensing platform is ensured to be constant in combination with the PID control unit, and it is ensured that the temperature of each culture platform is consistent; a thermopile sensor is used for monitoring heat change generated by metabolism of bacteria before and after antibiotic application, and a basis is provided for drug sensitivity analysis; a plurality of culture platforms are integrated, a plurality of bacterial samples are simultaneously cultured and subjected to heat measurement, the screening efficiency is greatly improved, and the device is particularly suitable for preliminary screening of drugs and rapid response evaluation of clinical samples.

Description

Technical Field

[0001] The present application relates to the technical field of bacterial drug sensitivity detection, and in particular to a high-throughput thermal sensing platform and a method for using the same. Background Art

[0002] In recent years, the study and detection of direct bacterial metabolic activity has been crucial for understanding numerous issues in life sciences and medicine. It also represents a significant research challenge and hotspot within the industry, offering promising applications in bacterial analysis, mechanistic studies, and drug susceptibility testing. In the biomedical field, it is well known that bacterial resistance has become a significant threat to global public health. From a clinical perspective, rapid and accurate bacterial susceptibility testing and the screening and characterization of drug-resistant strains are crucial for addressing this challenge. Currently, most methods used in hospitals and testing centers are still based on observation of bacterial growth. These methods are typically time-consuming (approximately 20 hours or longer) and cannot achieve rapid detection and analysis at the bacterial level. Conducting drug susceptibility testing, screening for drug-resistant strains, and understanding drug susceptibility mechanisms based on bacterial metabolic activity will play a crucial role in optimizing the use of existing antibiotics, developing new antibiotics, and slowing the spread of bacterial resistance. Summary of the Invention

[0003] This application aims to address at least one of the technical problems existing in the prior art. It provides a high-throughput thermal sensing platform and its use method. This platform can rapidly, accurately, highly sensitively, and at high throughput measure the minute heat changes generated by bacterial metabolism before and after the application of antibiotics in the early stages of bacterial growth, thereby detecting bacterial susceptibility to antibiotics and significantly shortening susceptibility testing time.

[0004] A high-throughput thermal sensing platform according to an embodiment of the first aspect of the present application includes:

[0005] base;

[0006] A culture carrier, the culture carrier is arranged on the base, and the culture carrier is provided with a culture platform;

[0007] a heat-insulating cavity assembly, the heat-insulating cavity assembly being arranged on the culture platform;

[0008] A top cover plate, the top cover plate being arranged on the thermal insulation cavity assembly;

[0009] culture room;

[0010] Thermopile sensors;

[0011] Among them, the base, culture platform, insulation cavity assembly, and top cover are stacked in sequence, the culture platform is provided with a concave cavity, the concave cavity is used to place the thermopile sensor, the insulation cavity assembly is provided with a accommodating cavity, the accommodating cavity is arranged corresponding to the concave cavity, and the culture chamber is arranged on the thermopile sensor and accommodated in the accommodating cavity.

[0012] The high-throughput thermal sensing platform according to the embodiment of the first aspect of the present application has at least the following beneficial effects:

[0013] The high-throughput thermal sensing platform of the present application includes a base, a culture carrier, an insulating cavity assembly, a top cover, a culture chamber, and a thermopile sensor. The base, culture carrier, insulating cavity assembly, and top cover are stacked and fixed in sequence. The culture carrier is used to fix the thermopile sensor and support the culture chamber. The insulating cavity assembly is used to accommodate the culture chamber and isolate the culture chamber from heat exchange with the outside world. The base and top cover are used to fix the high-throughput thermal sensing platform. The culture carrier is provided with a culture platform, and the culture platform is provided with a concave cavity for placing the thermopile sensor. The insulating cavity assembly is provided with a receiving cavity, which is arranged corresponding to the concave cavity. The culture chamber is arranged on and accommodated in the receiving cavity by the thermopile sensor. The thermopile sensor is used to measure tiny thermal changes in the culture chamber. The high-throughput thermal sensor platform of the present application uses a high-precision thermopile sensor to monitor tiny metabolic heat changes in the culture chamber in real time. It can efficiently and accurately measure the metabolic heat changes of bacteria before and after the application of antibiotics in the early stages of bacterial growth, thereby evaluating the drug sensitivity of bacteria. Compared with existing technologies, it greatly shortens the time of drug sensitivity testing and promotes rapid drug screening and analysis. By setting up the thermal insulation cavity component, the heat exchange between the culture chamber and the outside world is greatly reduced, external interference is reduced, and the sensitivity of the high-throughput thermal sensing platform for drug sensitivity testing and the accuracy of the test results are significantly improved.

[0014] According to some embodiments of the present application, the insulation cavity assembly includes an insulation cavity body and an insulation cavity cover plate, the insulation cavity cover plate is arranged on the insulation cavity body, the accommodating cavity is arranged through the insulation cavity body, the culture chamber is provided with a first through hole, the insulation cavity cover plate is provided with a second through hole and a pipe channel, the first through hole and the second through hole are arranged correspondingly, and the pipe channel is connected to the second through hole.

[0015] According to some embodiments of the present application, the top surface of the culture platform is provided with a first groove and a second groove, the bottom surface of the culture platform is provided with a third groove, and the bottom surface of the top cover is provided with a fourth groove. One end of the first groove and the third groove are both connected to the center of the culture platform, and the other end is connected to the circumference of the culture platform. One end of the second groove is connected to the concave cavity, and the other end is connected to the circumference of the culture platform. The fourth groove is connected to the center of the top cover, and the other end is connected to the circumference of the top cover. The first groove, the second groove, the third groove and the fourth groove are used to place temperature sensing components.

[0016] According to some embodiments of the present application, it also includes a heating upper cover, a first heating component and a second heating component, the heating upper cover is arranged between the insulation cavity assembly and the top cover plate, the first heating component is arranged between the base and the culture platform, and the second heating component is arranged between the heating upper cover and the insulation cavity assembly.

[0017] According to some embodiments of the present application, an air cavity is provided around the concave cavity, and the depth of the air cavity is greater than the depth of the concave cavity.

[0018] According to some embodiments of the present application, a plurality of the first through hole, the second through hole, and the tube channel are provided, and the first through hole, the second through hole, and the tube channel are provided in a one-to-one correspondence.

[0019] According to some embodiments of the present application, there are multiple culture platforms.

[0020] According to some embodiments of the present application, a control device is further included, wherein the control device is used to control the heating power of the first heating component and the second heating component.

[0021] The method for using the high-throughput thermal sensing platform according to the second embodiment of the present application includes the following steps:

[0022] Control the exchange of substances inside and outside the culture chamber;

[0023] Collect temperature data;

[0024] inputting the acquired temperature data into a control device of the high-throughput thermal sensing platform;

[0025] Control heating power to achieve dynamic temperature control;

[0026] Thermopile sensors detect changes in bacterial metabolic heat before and after antibiotics are applied;

[0027] Assess drug susceptibility.

[0028] According to some embodiments of the present application, an installation step is also included, and the installation step includes vertically stacking and installing the base, the culture carrier, the insulation cavity assembly, and the top cover.

[0029] The method for using the high-throughput thermal sensing platform according to the second embodiment of the present application has at least the following beneficial effects:

[0030] The method for using the high-throughput thermal sensing platform in this application includes controlling material exchange within and outside the culture chamber; collecting temperature data; inputting the acquired temperature data into a control device; controlling heating power to achieve dynamic temperature control; detecting metabolic heat changes using a thermopile sensor; and assessing drug susceptibility. Through full-process automated control and multi-point data collection, this method provides an efficient, accurate, and highly repeatable method for bacterial metabolic heat measurement and drug susceptibility testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The present application is further described below with reference to the accompanying drawings and embodiments, wherein:

[0032] Figure 1 This is a schematic structural diagram of a high-throughput thermal sensing platform according to an embodiment of the present application;

[0033] Figure 2 This is an exploded diagram of a high-throughput thermal sensing platform according to an embodiment of the present application;

[0034] Figure 3 for Figure 2 Explosion diagram from another angle;

[0035] Figure 4 This is a schematic structural diagram of the top surface of a base according to an embodiment of the present application;

[0036] Figure 5 This is a schematic structural diagram of the bottom surface of a base according to an embodiment of the present application;

[0037] Figure 6 This is a schematic structural diagram of the top surface of a culture carrier according to an embodiment of the present application;

[0038] Figure 7 This is a schematic structural diagram of the bottom surface of a culture carrier according to an embodiment of the present application;

[0039] Figure 8 This is a structural schematic diagram of the top surface of the heat-insulating cavity body according to one embodiment of the present application;

[0040] Figure 9 This is a structural schematic diagram of the bottom surface of the heat-insulating cavity body according to one embodiment of the present application;

[0041] Figure 10This is a structural schematic diagram of the top surface of the heat insulation cavity cover plate according to one embodiment of the present application;

[0042] Figure 11 This is a structural schematic diagram of the bottom surface of a heat-insulating cavity cover plate according to an embodiment of the present application;

[0043] Figure 12 This is a schematic structural diagram of the top surface of the heating upper cover according to an embodiment of the present application;

[0044] Figure 13 This is a schematic structural diagram of the bottom surface of the heating upper cover according to an embodiment of the present application;

[0045] Figure 14 This is a schematic structural diagram of the top surface of a top cover plate according to an embodiment of the present application;

[0046] Figure 15 This is a schematic structural diagram of the bottom surface of a top cover plate according to an embodiment of the present application;

[0047] Figure 16 This is a schematic structural diagram of a culture chamber according to an embodiment of the present application;

[0048] Figure 17 for Figure 16 A structural diagram from another angle;

[0049] Reference numerals:

[0050] Base 1; first wiring trough 11; base countersunk hole 12;

[0051] Culture carrier 2; cavity 21; first groove 22; second groove 23; third groove 24; culture carrier through hole 25; culture carrier positioning hole 26; culture carrier blind hole 27;

[0052] Thermal insulation cavity body 3; accommodating cavity 31; thermal insulation cavity body through hole 32; thermal insulation cavity body positioning hole 33;

[0053] Insulation cavity cover plate 4; second through hole 41; pipe channel 42; insulation cavity cover plate through hole 43; insulation cavity cover plate positioning hole 44;

[0054] Top cover plate 5; fourth groove 51; top cover plate countersunk hole 52;

[0055] Culture room 6;

[0056] Thermopile sensor 7;

[0057] Heating cover 8; second wiring groove 81; heating cover through hole 82. DETAILED DESCRIPTION

[0058] The following describes in detail embodiments of the present application. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application and are not to be construed as limiting the present application.

[0059] In the description of this application, it should be understood that if the terms "center", "middle", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", "circumferential" and the like appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application. In addition, features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of this application, unless otherwise specified, "multiple" means two or more.

[0060] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0061] Refer to the following Figures 1 to 17 The present invention describes a high-throughput thermal sensing platform and its use method in the embodiments of the present invention.

[0062] according to Figures 1 to 3 、 Figures 6 and 7As shown, a high-throughput thermal sensing platform according to one embodiment of the present application includes a base 1, a culture platform 2, an insulating cavity assembly, a top cover 5, a culture chamber 6, and a thermopile sensor 7. The base 1, culture platform 2, insulating cavity assembly, and top cover 5 are stacked and fixed in sequence, with the top surface of the base 1 in contact with the bottom surface of the culture platform 2, the top surface of the culture platform 2 in contact with the bottom surface of the insulating cavity assembly, and the bottom surface of the top cover 5 in contact with the top surface of the insulating cavity assembly. The base 1 is used to support the upper components. The culture carrier 2 is used to fix the thermopile sensor 7 and support the culture chamber 6. The insulation cavity assembly is used to accommodate the culture chamber 6 and isolate the culture chamber 6 from heat exchange with the outside world. The top surface of the culture carrier 2 is provided with a culture platform, and the culture platform is provided with a concave cavity 21. The concave cavity 21 is used to place the thermopile sensor 7. The insulation cavity assembly is provided with a receiving cavity 31. The receiving cavity 31 is arranged to correspond to and overlap with the concave cavity 21. The culture chamber 6 is set on the thermopile sensor 7 and accommodated in the receiving cavity 31. The thermopile sensor 7 can quickly capture and measure the tiny heat changes in the culture chamber 6. The top cover 5 is used to fix the high-throughput thermal sensing platform and seal the insulation cavity assembly. The base 1, the insulation cavity assembly and the top cover 5 together form a closed space for the receiving cavity, which effectively isolates the external interference, so that the temperature change in the culture chamber 6 is mainly determined by the heat generated by the bacteria. The heat generated by bacterial metabolism can be fully transferred to the thermopile sensor 7 for heat detection, thereby improving the accuracy of the drug sensitivity test.

[0063] The high-throughput thermal sensor platform of the present application monitors minute heat changes in the culture chamber 6 in real time through a high-precision thermopile sensor 7. It can efficiently and accurately measure the minute metabolic heat of bacteria before and after the application of antibiotics in the early stages of bacterial growth, thereby assessing the bacterial susceptibility to a certain antibiotic. Compared with existing technologies, this greatly shortens the time for drug susceptibility testing and promotes rapid drug screening and analysis. The provision of an insulating cavity assembly forms an effective insulation structure, greatly reducing heat exchange between the culture chamber 6 and the outside world, isolating internal heat from dissipating outward, and reducing external environmental temperature interference, significantly improving the sensitivity of the high-throughput thermal sensing platform for drug susceptibility testing and the accuracy of test results.

[0064] In some embodiments, the thermopile sensor 7 is formed by a plurality of thermocouples connected in series. The thermopile sensor 7 is configured as a flat structure. The shape of the cavity 21 matches the shape of the thermopile sensor 7 , and the thermopile sensor 7 can be firmly fixed in the cavity 21 .

[0065] In some embodiments, the culture chamber 6 is a rectangular parallelepiped structure, and the interior of the culture chamber 6 is a cavity for placing and culturing bacteria. The size of the culture chamber 6 matches the size of the thermopile sensor 7, and the height is 10 mm.

[0066] according to Figures 1 to 3 、 Figures 6 to 11As shown, in one embodiment of the present application, the thermal insulation cavity assembly includes a thermal insulation cavity body 3 and a thermal insulation cavity cover plate 4, the thermal insulation cavity cover plate 4 is arranged on the thermal insulation cavity body 3, and the bottom surface of the thermal insulation cavity cover plate 4 is in contact with the top surface of the thermal insulation cavity body 3. The accommodating cavity 31 passes through the top and bottom surfaces of the thermal insulation cavity body 3. The culture chamber 6 is provided with a first through hole, and the thermal insulation cavity cover plate 4 is provided with a second through hole 41 and a tube channel 42. The first through hole and the second through hole 41 are aligned and arranged to form a through passage, and the tube channel 42 is directly connected to the second through hole 41, so that the tube channel 42 is connected to the through passage. The culture chamber 6 exchanges substances with the external environment through the tube channel 42 and the through passage, so that the culture chamber 6 can exchange substances with the outside world while maintaining the stable insulation state of the high-throughput thermal sensing platform.

[0067] In some embodiments, the high-throughput thermal sensing platform further includes a microfluidic hose, which sequentially passes through the tube channel 42, the second through-hole 41, and the first through-hole to connect to the culture chamber 6. The microfluidic hose is a flexible conduit that connects the culture chamber 6 to transfer fluid. Microfluidic technology allows for precise control of fluid delivery and minimizes the impact of ambient temperature on the temperature of the culture chamber 6.

[0068] according to Figures 1 to 3 、 Figures 6 to 11 、 Figures 14 to 17 As shown, in one embodiment of the present application, the top surface of the culture platform 2 is provided with a first groove 22 and a second groove 23, the bottom surface of the culture platform 2 is provided with a third groove 24, and the bottom surface of the top cover 5 is provided with a fourth groove 51. One end of the first groove 22 connects to the central area of the top surface of the culture platform 2, and the other end connects to the circumference of the culture platform 2. One end of the second groove 23 connects to the vicinity of the concave cavity 21 of the culture platform on the top surface of the culture platform 2, and the other end connects to the circumference of the culture platform 2. One end of the third groove 24 connects to the central area of the bottom surface of the culture platform 2, and the other end connects to the circumference of the culture platform 2. One end of the fourth groove 51 connects to the central area of the bottom surface of the top cover 5, and the other end connects to the circumference of the top cover 5. The first groove 22, second groove 23, third groove 24, and fourth groove 51 are used to accommodate temperature sensing components. The first temperature sensing component is placed in the first groove 22, and the first temperature sensing component is used to obtain the temperature data of the central area of the culture platform 2; the second temperature sensing component is placed in the second groove 23, and the second temperature sensing component is used to obtain the temperature data near the culture platform; the third temperature sensing component is placed in the third groove 24, and the third temperature sensing component is used to obtain the temperature data of the bottom surface of the culture platform 2; the fourth temperature sensing component is placed in the fourth groove 51, and the fourth temperature sensing component is used to obtain the temperature data of the bottom surface of the top cover 5.

[0069] The installation of multiple temperature sensing components can monitor in real time whether the temperature of the high-throughput thermal sensing platform is uniformly and stably distributed locally and overall. The first temperature sensing component and the third temperature sensing component can jointly monitor the temperature data of the top and bottom surfaces, center and circumference of the culture platform 2. The second temperature sensing component and the thermopile sensor 7 can monitor in a coordinated manner and jointly detect the local temperature difference data of the culture platform and its vicinity. The third temperature sensing component and the fourth temperature sensing component jointly monitor the temperature data of the high-throughput thermal sensing platform as a whole. By analyzing and comparing the temperature data of the first temperature sensing component and the second temperature sensing component, the uniformity of the temperature distribution of the culture platform 2 and the possible temperature gradient are reflected, which helps to assess whether there is temperature unevenness in the culture environment and reduce the possibility of affecting the growth state of bacteria, thereby improving the accuracy of the drug sensitivity test results of the high-throughput thermal sensing platform.

[0070] The four temperature sensing components are arranged longitudinally from the center to the periphery, effectively collecting temperature data from different areas of the high-throughput thermal sensing platform, ensuring that temperature detection covers from the center to the edge and upper and lower layers, realizing all-round temperature balance monitoring, ensuring the rapidity and accuracy of temperature information transmission, and providing reliable data support for subsequent real-time temperature adjustment.

[0071] according to Figures 1 to 17 As shown, in one embodiment of the present application, the high-throughput thermal sensing platform further includes a heating cover 8, a first heating component and a second heating component. The heating cover 8 is arranged between the thermal insulation cavity assembly and the top cover plate 5, the top surface of the heating cover 8 is in contact with the bottom surface of the top cover plate 5, and the bottom surface of the heating cover 8 is in contact with the thermal insulation cavity assembly. The heating cover 8 is used to isolate the influence of the external environment on the high-throughput thermal sensing platform. The first heating component is arranged between the base 1 and the culture platform 2, and the second heating component is arranged between the heating cover 8 and the thermal insulation cavity assembly. The first heating component and the second heating component are used to provide a uniform and stable heat source for the high-throughput thermal sensing platform from the bottom and top, ensuring that the culture platform 2 can obtain continuous and stable heat input during the temperature control process. The temperature of the high-throughput thermal sensing platform is uniformly regulated by temperature adjustment through the first heating component and the second heating component.

[0072] The third temperature sensing component is also used to detect the temperature of the first heating component. The heating cover 8 also transfers heat generated by the second heating component, allowing the fourth temperature sensing component to detect the temperature of the second heating component. In conjunction with the thermally insulated cavity assembly, the heating cover 8 not only transfers heat but also limits external temperature interference, limiting the internal environment to the controlled heating component. This achieves precise dynamic temperature control, positively impacting the accuracy and reliability of the data measured by the thermopile sensor 7.

[0073] In some embodiments, the first heating component and the second heating component are heating sheets, specifically flat heating sheets.

[0074] In some embodiments, a first wiring groove 11 is provided on the top surface of the base 1, and the first heating component is routed through the first wiring groove 11; a second wiring groove 81 is provided on the bottom surface of the heating cover 8, and the second heating component is routed through the second wiring groove 81.

[0075] In some embodiments, the insulation cavity assembly includes an insulation cavity body 3 and an insulation cavity cover 4, the heating cover 8 is specifically arranged between the insulation cavity cover 4 and the top cover 5, and the second heating component is specifically arranged between the heating cover 8 and the insulation cavity cover 4.

[0076] In some embodiments, the base 1 is provided with a plurality of base countersunk holes 12; the bottom surface of the culture platform 2 is provided with a plurality of culture platform blind holes 27, and the top surface is provided with a plurality of culture platform through holes 25 and a plurality of culture platform positioning holes 26; the insulation cavity body 3 is provided with a plurality of insulation cavity body through holes 32 and a plurality of insulation cavity body positioning holes 33; the insulation cavity cover 4 is provided with a plurality of insulation cavity cover through holes 43 and a plurality of insulation cavity cover positioning holes 44; the heating cover 8 is provided with a plurality of heating cover through holes 82 and a plurality of heating cover 8 positioning holes; the top cover 5 is provided with a plurality of top cover countersunk holes 52.

[0077] The base countersunk hole 12 is used to correspond to the blind hole 27 of the culture platform, so that the culture platform 2 is firmly installed on the base 1; the culture platform positioning hole 26 is aligned with the insulation cavity main body positioning hole 33 to achieve precise docking and fixation of the culture platform 2 and the insulation cavity main body 3; the insulation cavity main body positioning hole 33 is aligned with the insulation cavity cover positioning hole 44 to enable the insulation cavity main body 3 and the insulation cavity cover 4 to fit tightly; the insulation cavity cover positioning hole 44 is aligned with the heating cover 8 positioning hole to ensure a firm structural fit between the insulation cavity cover 4 and the heating cover 8; the above positioning holes are connected by positioning pins. The culture platform through-hole 25, the insulation chamber body through-hole 32, the insulation chamber cover through-hole 43, the heating cover through-hole 82, and the top cover countersunk hole 52 extend through the formed channel. These through-holes are connected by countersunk screws to achieve longitudinal and hierarchical fixation of the components, ensuring a stable structure and accurate positioning after assembly of the high-throughput thermal sensing platform. In some embodiments, the positioning holes are specifically configured as threaded positioning holes.

[0078] In some embodiments, the base 1, the culture platform 2, the insulation cavity body 3, the insulation cavity cover 4, the heating cover 8 and the top cover 5 are all configured as flat cylindrical structures with the same diameter.

[0079] according to Figures 1 to 3As shown, in one embodiment of the present application, an air cavity is provided around the concave cavity 21, and the depth of the air cavity is greater than the depth of the concave cavity 21. At the same time, the culture chamber 6 is placed on the thermopile sensor 7 and accommodated in the accommodating cavity 31, but the culture chamber 6 is not directly in contact with the insulation cavity body 3. The air around the culture chamber 6 and the insulation cavity body 3 also form an air cavity. Two air cavities are arranged continuously along the culture chamber 6. The air cavity surrounds the culture chamber 6. The air cavity utilizes the low thermal conductivity of air to effectively isolate the conduction of heat, so that the air acts as an insulation barrier around the entire culture chamber 6, and can effectively block the heat transfer between the insulation cavity and the culture chamber 6. By establishing multi-layer heat separation, the air cavity heat isolation and the insulation cavity body 3 heat isolation, the heat conduction path is optimized, the influence of the ambient temperature on the temperature of the culture chamber 6 is reduced, and it is ensured that the heat generated by bacterial metabolism can be fully transferred to the thermopile sensor 7. The accuracy and stability of the thermopile sensor 7 in measuring the small metabolic heat changes of bacteria in the culture chamber 6 are improved, providing reliable data for subsequent drug sensitivity analysis.

[0080] The high-throughput thermal sensing platform is connected to the bacterial culture chamber 6 through an air cavity and a microfluidic soft pipe, which can minimize the impact of the ambient temperature on the temperature of the culture chamber 6 and significantly improve the accuracy of the drug sensitivity test.

[0081] In some embodiments, the size of the accommodating cavity 31 is consistent with the size of the air cavity surrounding the cavity 21 .

[0082] In some embodiments, the air cavity is specifically an air cavity with a depth of 6.6 mm and a width of 1.95 mm around the concave cavity 21, which can form a stable heat insulation barrier, significantly reduce heat conduction through the structure, and reduce the impact of the internal temperature of the culture chamber 6 being lost to the outside world.

[0083] according to Figures 1 to 15 As shown, in one embodiment of the present application, a plurality of the first through holes, the second through holes 41, and the tube channel 42 are provided. Each group of the first through holes, the second through holes 41, and the tube channel 42 is provided in a one-to-one correspondence, and the through passages and the tube channels are connected to form an exchange channel. Through the multiple groups of exchange channels, substances in the culture chamber 6 can be respectively transported to and discharged from the culture chamber 6.

[0084] In some embodiments, the exchange channels are specifically arranged into three groups, and the three first through holes on the top of the culture chamber 6 include vents, access holes, and exhaust holes. The microfluidic hose passes through the corresponding tube channel 42, the second through hole 41, and the vent hole of the culture chamber 6 on the insulation cavity cover 4 in sequence, to ensure air circulation in the culture chamber 6, maintain appropriate oxygen and carbon dioxide concentrations, and promote bacterial growth. The microfluidic hose passes through the corresponding tube channel 42, the second through hole 41, and the access hole in sequence, which facilitates the experimenter to add culture medium, reagents, or collect samples into the culture chamber 6. The microfluidic hose passes through the corresponding tube channel 42, the second through hole 41, and the exhaust hole in sequence, to adjust the gas pressure in the culture chamber 6 and remove accumulated waste gas to prevent it from affecting the culture environment. Three sets of independent exchange channels and corresponding microfluidic hoses realize the separate transmission of air, reagents and waste gas. The flexibility of the hoses and microfluidic technology are used to ensure the precise delivery of fluids in each channel and the precise control of the bacterial culture environment. The gas pressure in the culture chamber 6 is adjusted to avoid the accumulation of waste gas in the culture chamber 6. It can also effectively reduce heat loss, so that the culture chamber 6 always maintains a stable and suitable culture environment, which is conducive to bacterial growth and accurate collection of experimental data, ensuring that the experimental operation and internal temperature environment control are in an ideal state.

[0085] Through the synergistic effect of the precisely docked first through hole, second through hole 41, tube channel 42 and three microfluidic hoses, not only the precise transmission and environmental control of each exchange channel are ensured, but also the good thermal insulation design can minimize the interference of external temperature fluctuations on the internal temperature of the culture chamber 6, ensuring the precise measurement of the tiny metabolic heat changes of bacteria in the culture chamber 6, and providing reliable data for subsequent drug sensitivity evaluation.

[0086] according to Figures 1 to 17 As shown, in one embodiment of the present application, the culture platform is set to multiple. Multiple culture platforms are integrated on the culture carrier 2, and can simultaneously culture and measure metabolic heat of multiple bacterial samples. There is an appropriate interval between each culture platform to ensure that when bacterial culture, temperature detection and reagent operation are carried out on different culture platforms, they will not interfere with each other, thereby improving the reliability and stability of the test data and facilitating overall heat insulation and temperature control management. Each culture platform can work independently and perform metabolic heat detection independently, thereby simultaneously performing rapid screening of multiple groups of samples, data collection, and drug sensitivity analysis. The use of multiple culture platforms can provide flexibility, facilitate the configuration of special functions for individual culture platforms according to different experimental requirements, and realize customized operations and data comparative analysis. This parallel processing significantly improves the test efficiency, reflecting the high-throughput characteristics of the high-throughput thermal sensing platform of the present application.

[0087] In some embodiments, the number of culture platforms is eight, and the eight culture platforms are equally divided on the circumference of the culture carrier 2 .

[0088] according to Figures 1 to 17 As shown, in one embodiment of the present application, the high-throughput thermal sensing platform further includes a control device, which is used to dynamically adjust and control the heating power of the first heating component and the second heating component, thereby achieving constant temperature without being disturbed by the external environment, and ensuring that the temperature of each culture platform is stable and consistent.

[0089] In some embodiments, the control device includes a PID control unit. The first temperature sensing component, the second temperature sensing component, the third temperature sensing component, and the fourth temperature sensing component are distributed at the top, bottom, center, and near the culture chamber 6 of the high-throughput thermal sensing platform, forming a central, circumferential, and longitudinal temperature detection network to achieve global monitoring of the temperature of different areas. By obtaining temperature data from the temperature detection network of the high-throughput thermal sensing platform, the temperature data is input into the PID control unit. Based on the obtained temperature data, the PID control unit can independently adjust the first heating component and the second heating component, control the heating power of the first heating component and the second heating component, and thus perform temperature regulation to ensure that the temperature of the high-throughput thermal sensing platform remains stable at different heights and areas, thereby meeting the constant environment required for metabolic heat measurement on each culture platform. In some embodiments, the control device can also adjust the heating power of the heating component in real time according to changes in ambient temperature, so that the high-throughput thermal sensing platform can maintain an internal constant temperature state even in complex or unstable environments, thereby improving the overall robustness and applicability.

[0090] In some embodiments, the control device also includes a high-throughput and precise heat measurement unit. Based on the PID control unit, while maintaining stable constant temperature conditions, the high-throughput and precise heat measurement unit obtains the temperature data of the thermopile sensors 7 of multiple culture platforms, and simultaneously performs multiple bacterial sample cultures and metabolic heat measurements, processes in parallel, and performs high-throughput and precise metabolic heat measurements. The temperature difference is converted into an electrical signal, which is recorded and processed by the signal acquisition system to form curve data of temperature changes over time.

[0091] In some embodiments, the control device includes a fuzzy control system and an adaptive control system.

[0092] according to Figures 1 to 17 As shown, in one embodiment of the present application, a method for using a high-throughput thermal sensing platform includes the following steps:

[0093] S1. Controlling the exchange of substances inside and outside the culture chamber 6;

[0094] S2. Collect temperature data;

[0095] S3. The temperature data obtained is input into the control device;

[0096] S4. Control heating power to achieve dynamic temperature control;

[0097] S5. Thermopile sensor 7 detects changes in metabolic heat of bacteria before and after application of antibiotics;

[0098] S6. Assess drug susceptibility.

[0099] The specific steps are as follows:

[0100] S1. Controlling the exchange of substances inside and outside culture chamber 6: Microfluidic hoses are connected to the vents, access ports, and exhaust ports of culture chamber 6 to establish an exchange pathway with the external environment. Microfluidic technology utilizes tiny microfluidic hoses to control fluid flow, manipulating the liquid environment at a microscopic scale. Air is introduced through the vents to maintain appropriate oxygen and carbon dioxide concentrations; culture medium or drugs are added to culture chamber 6 through the access ports; and exhaust ports are used to remove waste gases or regulate internal pressure to ensure normal bacterial growth and a stable reaction environment.

[0101] S2. Collecting Temperature Data: Activate the temperature sensing components of the high-throughput thermal sensing platform, located at different locations, including the top and bottom, the center, and near chamber 6. Each temperature sensing component collects real-time temperature data at its location to assess temperature distribution and environmental changes.

[0102] S3. Inputting the acquired temperature data into the control device: All collected temperature data is fed into the control device, forming a temperature detection network. The control device compares temperature changes in different areas, identifies temperature gradients and fluctuation trends, and provides a basis for subsequent control.

[0103] S4. Controlling Heating Power for Dynamic Temperature Control: Based on the received temperature data, the control device uses a PID control algorithm to adjust the output power of the first and second heating components. This real-time adjustment of the heating component output power enables automatic and stable temperature control, ensuring that the entire high-throughput thermal sensing platform maintains a constant temperature and avoids external environmental interference.

[0104] S5. Thermopile sensor 7 detects metabolic heat changes: Heat generated by bacterial metabolism in culture chamber 6 is transferred to thermopile sensor 7 below via thermal conduction. Thermopile sensor 7 converts the temperature difference into an electrical signal, which is recorded and processed by the signal acquisition system, forming a curve showing temperature changes over time.

[0105] S6. Drug Susceptibility Assessment: Based on the heat curve feedback from thermopile sensor 7, analyze the differences in bacterial metabolic intensity when different drugs or conditions are added. Heat increases or decreases can be used as a sign of drug activation or inhibition of bacterial metabolism, thereby providing a preliminary assessment of bacterial sensitivity or resistance to a particular antibiotic, enabling rapid drug susceptibility assessment.

[0106] The high-throughput thermal sensing platform and its use method of the present application can realize real-time monitoring of multi-point temperature and combine with a PID control unit to ensure that the temperature of the high-throughput thermal sensing platform is constant, avoid temperature fluctuations affecting the measurement accuracy, and ensure that the temperature of each culture platform is stable and consistent; through the thermopile sensor 7, the tiny heat changes generated by bacterial metabolism are monitored in real time, and its heat changes are accurately captured to provide a reliable basis for drug sensitivity analysis; it also integrates multiple culture platforms and can support the simultaneous cultivation and heat measurement of multiple bacterial samples, greatly improving screening efficiency, and is especially suitable for initial drug screening and rapid response evaluation of clinical samples. The use method of the high-throughput thermal sensing platform of the present application provides an efficient, accurate and repeatable method for bacterial metabolic heat measurement and drug sensitivity testing through full-process automatic control and multi-point data collection.

[0107] according to Figures 1 to 17 As shown, in one embodiment of the present application, an installation step is also included, and the installation step includes vertically stacking and installing the base 1, the culture carrier 2, the insulation cavity assembly, and the top cover 5.

[0108] The specific steps include: vertically stacking and installing the base 1, the first heating component, the culture platform 2, the heat-insulating cavity body 3, the heat-insulating cavity cover 4, the second heating component, and the top cover 5 in sequence.

[0109] More specific steps are as follows:

[0110] First, place the base 1 horizontally as a basic support. Place the first heating component on the top surface of the base 1, and the power supply line of the first heating component is led out through the first wiring groove 11 of the base 1. Ensure that the subsequent connection with the control system is smooth. Embed the third temperature sensing component in the third groove 24 on the ground of the culture carrier 2. Then place the culture carrier 2 on the ground of the base 1. Align the countersunk hole of the base 1 and the threaded blind hole of the culture carrier 2, and fix them with countersunk screws to form a firm connection. Install the thermopile sensor 7 in the concave cavity 21 on the culture carrier 2. Install the first temperature sensing component in the first groove 22, and install the second temperature sensing component in the second groove 23. Insert the locating pin into the culture carrier positioning hole 26, and then place the insulation cavity body 3 corresponding to the top surface of the culture carrier 2. By aligning the insulation cavity positioning hole and the carrier positioning hole, the insulation cavity body 3 and the culture carrier 2 are accurately docked and fixed in position. Place the culture chamber 6 in the accommodating cavity just above the thermopile sensor 7, and the three first through holes on the top of each culture chamber 6 are connected to three microfluidic hoses respectively. The other end of the microfluidic hose passes through the second through hole 41 and the pipe channel 42 of the insulation cavity cover 4, and is led out to the outside for ventilation, reagents and exhaust. Align the insulation cavity cover positioning hole 44 with the insulation cavity main body positioning hole 33, and fix the insulation cavity cover 4 on the top surface of the insulation cavity main body 3 by the positioning pin. Place the second heating component on the bottom surface of the heating cover 8, and wire it to the external control device through the second wiring groove 81. Then place the heating cover 8 on the insulation cavity cover 4. Place the top cover 5 on the top surface of the heating cover 8, ensuring that the top cover countersunk hole 52, the heating cover through hole 82, the insulation cavity cover through hole 43 and the insulation cavity through hole remain aligned. Use countersunk screws to pass through the above-mentioned through holes from the top in sequence, and finally screw the screws into the threaded through holes of the culture carrier 2, locking all parts firmly longitudinally to complete the overall assembly of the platform.

[0111] The various components of the high-throughput thermal sensing platform of the present application are precisely aligned and mechanically fixed through threaded holes, through holes, positioning holes, etc., forming a solid and unified structural framework to ensure that the platform remains reliable during multiple uses and transportation. By sequentially nesting and assembling the heating components, thermopile sensors 7, air cavities and thermal insulation cavity components, an efficient heat conduction path and temperature control structure are formed to provide a stable thermal environment for subsequent precise heat detection. The modular assembly design of each layer makes the high-throughput thermal sensing platform easy to disassemble and replace, such as the thermopile sensor 7 or the culture chamber 6. The replacement operation is convenient, which greatly improves the actual application efficiency and service life. During the assembly process, the various components are accurately matched to avoid offset, ensuring that the thermal signal can be effectively transmitted to each temperature sensing component, and improving the measurement sensitivity and consistency.

[0112] In the description of this specification, if the reference terms "one embodiment," "some examples," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples" appear, it means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples.

[0113] The above describes the implementation methods of the present application in detail in conjunction with the accompanying drawings, but the present application is not limited to the above implementation methods. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the technical field without departing from the purpose of the present application.

Claims

1. A high-throughput thermal sensing platform, characterized by: include base; A culture carrier, the culture carrier is arranged on the base, and the culture carrier is provided with a culture platform; a heat-insulating cavity assembly, the heat-insulating cavity assembly being arranged on the culture platform; A top cover plate, the top cover plate being arranged on the thermal insulation cavity assembly; culture room; Thermopile sensors; Among them, the base, the culture platform, the insulation cavity assembly, and the top cover are stacked in sequence, the culture platform is provided with a concave cavity, the concave cavity is used to place the thermopile sensor, the insulation cavity assembly is provided with a accommodating cavity, the accommodating cavity is arranged corresponding to the concave cavity, and the culture chamber is arranged on the thermopile sensor and accommodated in the accommodating cavity.

2. The high-throughput thermal sensing platform according to claim 1, characterized in that: The thermal insulation cavity assembly includes a thermal insulation cavity body and a thermal insulation cavity cover plate, the thermal insulation cavity cover plate is arranged on the thermal insulation cavity body, the accommodating cavity is arranged through the thermal insulation cavity body, the culture chamber is provided with a first through hole, the thermal insulation cavity cover plate is provided with a second through hole and a pipe channel, the first through hole and the second through hole are arranged correspondingly, and the pipe channel is connected to the second through hole.

3. The high-throughput thermal sensing platform according to claim 1, characterized in that: The top surface of the culture platform is provided with a first groove and a second groove, the bottom surface of the culture platform is provided with a third groove, and the bottom surface of the top cover is provided with a fourth groove. One end of the first groove and the third groove are connected to the center of the culture platform, and the other end is connected to the circumference of the culture platform. One end of the second groove is connected to the concave cavity, and the other end is connected to the circumference of the culture platform. The fourth groove is connected to the center of the top cover, and the other end is connected to the circumference of the top cover. The first groove, the second groove, the third groove and the fourth groove are used to place temperature sensing components.

4. The high-throughput thermal sensing platform according to claim 1, wherein: It also includes a heating cover, a first heating component and a second heating component. The heating cover is arranged between the insulation cavity assembly and the top cover plate, the first heating component is arranged between the base and the culture platform, and the second heating component is arranged between the heating cover and the insulation cavity assembly.

5. The high-throughput thermal sensing platform according to claim 1, characterized in that: An air cavity is provided around the concave cavity, and the depth of the air cavity is greater than the depth of the concave cavity.

6. The high-throughput thermal sensing platform according to claim 2, characterized in that: A plurality of the first through holes, the second through holes and the tube channels are provided, and the first through holes, the second through holes and the tube channels are provided in a one-to-one correspondence.

7. The high-throughput thermal sensing platform according to claim 1, characterized in that: The culture platforms are provided in plurality.

8. The high-throughput thermal sensing platform according to claim 4, characterized in that: The device further comprises a control device for controlling the heating power of the first heating component and the second heating component.

9. A method for using a high-throughput thermal sensing platform, characterized in that: The high-throughput thermal sensing platform is a high-throughput thermal sensing platform according to any one of claims 1 to 8, comprising the following steps: Control the exchange of substances inside and outside the culture room; Collect temperature data; inputting the acquired temperature data into a control device of the high-throughput thermal sensing platform; Control heating power to achieve dynamic temperature control; Thermopile sensors detect changes in bacterial metabolic heat before and after antibiotics are applied; Assess drug susceptibility.

10. The method for using the high-throughput thermal sensing platform according to claim 9, characterized in that: The method further includes an installation step, which includes vertically stacking and installing the base, the culture carrier, the thermal insulation cavity assembly, and the top cover.

Images