Automatic preparation and application platform for CAR-NK cells

Abstract

The invention belongs to the technical field of biomedical engineering, and particularly relates to a CAR-NK cell automatic preparation and application platform, which comprises a tank body, an operation table, a lifting adjustment assembly I, a hollow ring, a lifting adjustment assembly II, a rotation adjustment assembly, a plurality of hooks, a hollow shaft, a mixing tank, a sealing tank, a filter cartridge, a stirring assembly, a valve I, a liquid separation assembly and a plurality of culture containers, a circular opening is formed in the bottom of the tank body, two supporting frames are fixedly installed at the bottom of the tank body, the first lifting adjusting assembly is arranged on the two supporting frames and connected with the tank body, the first lifting adjusting assembly is matched with the circular opening, and the operation table is arranged on the first lifting adjusting assembly. The CAR-NK cell preparation device has the advantages of being reasonable in design, convenient to operate, safe, reliable and high in automation degree, automatic mixing, filtering, separating, stirring and other operations of CAR-NK cells can be achieved, the preparation efficiency of the CAR-NK cells can be effectively improved, and the preparation efficiency and the preparation quality are further improved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical engineering technology, and in particular to an automated preparation and application platform for CAR-NK cells. Background Art

[0002] CAR-NK cell therapy has shown great potential in areas such as cancer treatment. It genetically modifies NK cells to enable them to specifically recognize and kill tumor cells. However, the current preparation process of CAR-NK cells is plagued by numerous problems.

[0003] In existing technologies, CAR-NK cell production relies heavily on manual labor, which is labor-intensive and inefficient. Manual labor makes it difficult to precisely control various parameters in the production process, resulting in poor consistency and stability in cell production, seriously affecting the production results. For example, during the cell culture process, manual labor cannot accurately control key parameters such as temperature, pH, and dissolved oxygen in the culture environment, which can easily inhibit cell growth or even cause cell death.

[0004] At the same time, the handling of tools and the dispensing of preparation solutions during the preparation process are not convenient. Accessing tools often requires operators to frequently enter and exit the sterile environment, increasing the risk of contamination and taking a long time. Traditional dispensing methods have a low degree of automation, making it difficult to quickly and accurately distribute the preparation solution to multiple culture vessels, greatly limiting the improvement of preparation efficiency.

[0005] Furthermore, existing preparation equipment and methods lack systematicity and integration, with each operating step operating independently and unable to achieve efficient collaborative operation. This makes the entire preparation process cumbersome and complex, making it difficult to meet the needs of large-scale clinical applications for CAR-NK cell preparation.

[0006] Therefore, there is an urgent need for an automated preparation and application platform that can realize the automated preparation of CAR-NK cells, facilitate the removal and placement of tools and the separation of preparation liquids, improve the preparation efficiency and effect, and reduce the labor intensity of operators. Summary of the Invention

[0007] The purpose of the present invention is to address the shortcomings of the existing technology and propose an automated preparation and application platform for CAR-NK cells.

[0008] The above technical objectives of the present invention are achieved through the following technical solutions: a CAR-NK cell automated preparation and application platform, including a tank body, an operating table, a lifting adjustment component 1, a hollow ring, a lifting adjustment component 2, a rotation adjustment component, multiple hooks, a hollow shaft, a mixing tank, a sealing tank, a filter cartridge, a stirring component, a valve 1, a liquid separation component and multiple culture containers;

[0009] The bottom of the tank body is provided with a circular opening, and two support frames are fixedly installed on the bottom of the tank body. The lifting adjustment component 1 is arranged on the two support frames and connected to the tank body, and the lifting adjustment component 1 is adapted to the circular opening. The operating table is arranged on the lifting adjustment component 1, and the multiple culture containers are all snap-fitted and mounted on the top side of the operating table.

[0010] The hollow ring is slidably mounted on the inner side wall of the tank body, the lifting adjustment component 2 is arranged on the tank body and connected to the hollow ring, the rotation adjustment component is arranged on the bottom inner wall of the hollow ring, and a plurality of hooks are arranged on the rotation adjustment component;

[0011] The hollow shaft is rotatably mounted on the top inner wall of the tank body, and the top end of the hollow shaft extends above the tank body and is sealed and rotatably mounted with a feed pipe, the mixing tank is sealed and rotatably mounted on the hollow shaft and fixedly connected to the bottom inner wall of the tank body, and the stirring assembly is arranged in the mixing tank, the sealed tank is fixedly mounted on the bottom of the mixing tank and is sealed and rotatably connected to the hollow shaft, the filter cartridge is fixedly mounted on the hollow shaft and is located in the sealed tank, the valve is fixedly mounted on the hollow shaft and is located between the mixing tank and the sealed tank, a plurality of upper discharge holes, a plurality of lower discharge holes, a plurality of upper feed holes and a plurality of lower feed holes are provided on the hollow shaft, the plurality of upper discharge holes and the plurality of upper feed holes are all located in the mixing tank, the plurality of lower feed holes and the plurality of lower discharge holes are respectively located in the sealed tank and the filter cartridge, an upper partition and a lower partition are fixedly mounted in the hollow shaft, and the upper partition is located between the plurality of upper discharge holes and the plurality of upper feed holes, and the lower partition is located between the plurality of lower feed holes and the plurality of lower discharge holes;

[0012] A housing is fixedly mounted on the top of the tank, a motor 3 is fixedly mounted on the housing, and a transmission gear is fixedly sleeved on the output shaft and the hollow shaft of the motor 3, and the two transmission gears are meshed with each other;

[0013] The liquid separation component is arranged at the bottom end of the hollow shaft and is adapted to a plurality of culture containers. A plurality of multi-dimensional robotic arms are fixedly mounted on the inner side wall of the tank body.

[0014] Preferably, the lifting and adjusting component includes a support seat, two guide rails, two electric cylinders and two guide blocks. The guide blocks are slidably installed in the two support frames, the guide rails are slidably installed on the side where the two guide blocks are close to each other, and the same support seat is fixedly installed on the side where the two guide rails are close to each other. The operating table is fixedly installed on the support seat, and electric cylinders are fixedly installed on both sides of the tank body. The working ends of the two electric cylinders are fixedly connected to the corresponding guide blocks.

[0015] Preferably, the cross section of the support seat is convex.

[0016] Preferably, a handle is fixedly mounted on the front side of the support seat.

[0017] Preferably, the second lifting and adjusting component includes two inner thread sleeves, two screw rods, two pulleys and a belt. Two inner thread sleeves are fixedly installed on the top of the hollow ring. Screw rods are threadedly installed in the two inner thread sleeves. The two screw rods are rotatably connected to the top inner wall of the tank body. The top ends of the two screw rods extend to the top of the tank body and are fixedly sleeved with pulleys. The same belt is connected to the two pulleys for transmission.

[0018] Preferably, the lifting and adjusting component 2 also includes a motor 1, a driving gear and a driven gear. The motor 1 is fixedly installed on the top of the shell. The output shaft of the motor 1 extends into the shell and is fixedly sleeved with the driving gear. The driven gear is fixedly sleeved on the screw rod close to the motor 1, and the driving gear is meshed with the driven gear.

[0019] Preferably, the rotation adjustment component includes motor 2, a driving gear and a gear disc. The gear disc is rotatably mounted on the bottom inner wall of the hollow ring, and motor 2 is fixedly mounted on the bottom inner wall of the hollow ring. A driving gear is fixedly sleeved on the output shaft of motor 2, and the driving gear is engaged with the gear disc, and multiple hooks are fixed on the bottom side of the gear disc.

[0020] Preferably, the stirring assembly includes a plurality of stirring rods, and the plurality of stirring rods are fixedly mounted on the inner side wall of the mixing tank, and the plurality of stirring rods are radially arranged with the hollow shaft as the center.

[0021] Preferably, the liquid separation component includes multiple branch pipes and multiple valves 2, and the bottom end of the hollow shaft is radially fixed with multiple branch pipes, and the ends of the multiple branch pipes away from the hollow shaft extend to the position directly above the corresponding culture container and are respectively fixed with valves 2.

[0022] Preferably, a plurality of observation windows are provided on the tank body.

[0023] The beneficial effects of the present invention are:

[0024] By cooperating with the provided tank body, operating table, lifting adjustment component 1, hollow ring, lifting adjustment component 2, rotation adjustment component, multiple hooks, hollow shafts, mixing tank, sealing tank, filter cartridge, stirring component, valve 1, liquid separation component and multiple culture containers, the present invention can realize the automated preparation of CAR-NK cells, and can facilitate the convenient taking and placing of tools required for use in the preparation process, and can facilitate the liquid separation operation of the preparation liquid, thereby greatly improving the preparation efficiency and preparation effect, and has a high degree of automation, reducing the labor intensity of the operator.

[0025] By setting the lifting adjustment component 1, the operating table can be controlled to rise and fall, thereby facilitating the control of the operating table to enter and exit the tank through the circular opening position, thereby facilitating operations such as taking and placing the culture container on the operating table.

[0026] By setting up the lifting and adjusting component 2, the height of the hollow ring can be adjusted, which is convenient for adjusting the height of the tool hung on the hook. At the same time, by setting up the rotating adjusting component, the tool on the hook can be controlled to adjust its position as needed, so that the multi-dimensional robotic arm can perform relevant operations by taking and placing it without manual operation. By setting up the stirring component, the stirring effect can be achieved when the hollow shaft rotates and the material is injected into the mixing tank, thereby improving the mixing effect. In addition, the filter cartridge and the sealing tank can be used to centrifuge the prepared liquid after stirring and mixing, and cooperate with the liquid separation component to separate the processed prepared liquid, thereby improving the preparation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0028] Figure 1 This is a schematic diagram of the three-dimensional structure of a CAR-NK cell automated preparation and application platform proposed in the present invention;

[0029] Figure 2 for Figure 1 Schematic diagram of the cross-sectional structure;

[0030] Figure 3 It is a schematic diagram of a partial three-dimensional structure of the present invention;

[0031] Figure 4 This is a schematic diagram of the structure of the lifting and adjusting component 1, the operating table and the culture container part proposed in the present invention;

[0032] Figure 5 This is a schematic structural diagram of the rotation adjustment component of the present invention;

[0033] Figure 6 for Figure 5 Schematic diagram of the structure of part A;

[0034] Figure 7 This is a schematic structural diagram of the hollow shaft, upper feed hole, upper discharge hole, lower feed hole, lower discharge hole, feed pipe, valve 1 and liquid separation component proposed in the present invention;

[0035] Figure 8 for Figure 7 Schematic diagram of the cross-sectional structure.

[0036] In the figure: 1, tank body; 101, observation window; 102, housing; 11, support frame; 2, operating table; 201, culture container; 21, support base; 22, guide rail; 23, electric cylinder; 24, guide block; 3, hollow ring; 301, inner thread sleeve; 302, screw rod; 303, pulley; 304, belt; 305, motor 1; 306, driving gear; 307, driven gear; 31, gear plate; 311, motor 2; 31 2. Drive gear; 32. Hook; 4. Hollow shaft; 401. Upper partition; 402. Lower partition; 403. Upper discharge hole; 404. Lower discharge hole; 405. Upper feed hole; 406. Lower feed hole; 41. Valve 1; 42. Branch pipe; 421. Valve 2; 43. Feed pipe; 44. Motor 3; 441. Transmission gear; 5. Mixing tank; 501. Stirring rod; 6. Sealing tank; 61. Filter cartridge; 7. Multi-dimensional robotic arm. DETAILED DESCRIPTION

[0037] The technical solutions of the present invention will be described clearly and completely below with reference to specific embodiments. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are also within the scope of protection of the present invention.

[0038] Reference Figure 1-8 A CAR-NK cell automated preparation and application platform includes a tank body 1, an operating table 2, a hollow ring 3, multiple hooks 32, a hollow shaft 4, a mixing tank 5, a sealing tank 6, a filter cartridge 61, a valve 41 and multiple culture containers 201. The bottom of the tank body 1 is provided with a circular opening, two support frames 11 are fixedly installed at the bottom of the tank body 1, and multiple multi-dimensional robotic arms 7 are fixedly installed on the inner wall of the tank body 1. The setting of the multi-dimensional robotic arms 7 is intended to replace manual related operations. A guide block 24 is slidably installed in each of the two support frames 11, and a guide block 24 is slidably installed on the side close to each other. Rail 22, the side where the two guide rails 22 are close to each other is fixedly installed with the same support base 21, the operating table 2 is fixedly installed on the support base 21, and electric cylinders 23 are fixedly installed on both sides of the tank body 1. The working ends of the two electric cylinders 23 are respectively fixedly connected to the corresponding guide blocks 24, which can control the lifting of the operating table 2, thereby facilitating the control of the operating table 2 in and out of the tank body 1 through the circular opening. In order to achieve the closure of the circular opening when the support base 21 rises to abut against the inner wall of the bottom of the tank body 1, the cross-section of the support base 21 is convex, and multiple culture containers 201 are snap-fitted and installed on the top side of the operating table 2;

[0039] A housing 102 is fixedly mounted on the top of the tank 1, and a motor 3 44 is fixedly mounted on the housing 102. A transmission gear 441 is fixedly sleeved on the output shaft of the motor 3 44 and the hollow shaft 4, and the two transmission gears 441 are meshed with each other.

[0040] The hollow ring 3 is slidably mounted on the inner wall of the tank body 1, and two inner thread sleeves 301 are fixedly mounted on the top of the hollow ring 3. Screw rods 302 are threadedly mounted in the two inner thread sleeves 301. The two screw rods 302 are rotatably connected to the top inner wall of the tank body 1. The top ends of the two screw rods 302 extend above the tank body 1 and are fixedly sleeved with pulleys 303. The two pulleys 303 are connected to the same belt 304 for transmission. When any one of the two screw rods 302 rotates, the other screw rod 302 can be driven to rotate synchronously, thereby achieving The height of the hollow ring 3 is now adjusted, which makes it easier to adjust the height of the tools hung on the hook 32, and thus facilitates the picking and placing and related operations by the multi-dimensional robot arm 7 without manual operation. A motor 1 305 is fixedly installed on the top of the housing 102, and the output shaft of the motor 1 305 extends into the housing 102 and is fixedly sleeved with a driving gear 306. A driven gear 307 is fixedly sleeved on the screw rod 302 near the motor 1 305. The driving gear 306 is meshed with the driven gear 307, and can drive the screw rod 302 to rotate as needed;

[0041] A toothed disc 31 is rotatably mounted on the inner wall of the bottom of the hollow ring 3. A second motor 311 is fixedly mounted on the inner wall of the bottom of the hollow ring 3. A driving gear 312 is fixedly sleeved on the output shaft of the second motor 311. The driving gear 312 is engaged with the toothed disc 31. A plurality of hooks 32 are fixed to the bottom side of the toothed disc 31. The position of the tools on the hooks 32 can be controlled and adjusted as needed, thereby facilitating the picking and placing operation of the multi-dimensional robot arm 7.

[0042] The hollow shaft 4 is rotatably mounted on the top inner wall of the tank body 1, and the top end of the hollow shaft 4 extends above the tank body 1 and is sealed and rotatably mounted with a feed pipe 43. The mixing tank 5 is sealed and rotatably mounted on the hollow shaft 4 and is fixedly connected to the bottom inner wall of the tank body 1. A plurality of stirring rods 501 are fixedly mounted on the inner side wall of the mixing tank 5. The plurality of stirring rods 501 are radially arranged based on the hollow shaft 4 as the center, and can achieve a stirring effect when the hollow shaft 4 rotates and fills the mixing tank 5.

[0043] The sealing tank 6 is fixedly mounted on the bottom of the mixing tank 5 and is sealed and rotatably connected to the hollow shaft 4. The filter cartridge 61 is fixedly mounted on the hollow shaft 4 and is located in the sealing tank 6. The valve 41 is fixedly mounted on the hollow shaft 4 and is located between the mixing tank 5 and the sealing tank 6 to control the delivery and adjustment of the prepared liquid between the mixing tank 5 and the sealing tank 6. The hollow shaft 4 is provided with a plurality of upper discharge holes 403, a plurality of lower discharge holes 404, a plurality of upper feed holes 405 and a plurality of lower feed holes 406. 6. The multiple upper discharge holes 403 and the multiple upper feed holes 405 are both located in the mixing tank 5, the multiple lower feed holes 406 and the multiple lower discharge holes 404 are respectively located in the sealing tank 6 and the filter cartridge 61, and the upper partition 401 and the lower partition 402 are fixedly installed in the hollow shaft 4, and the upper partition 401 is located between the multiple upper discharge holes 403 and the multiple upper feed holes 405, and the lower partition 402 is located between the multiple lower feed holes 406 and the multiple lower discharge holes 404;

[0044] The bottom end of the hollow shaft 4 is radially fixed with multiple branch pipes 42, and the multiple branch pipes 42 extend from one end of the hollow shaft 4 to the position directly above the corresponding culture container 201 and are fixed with valve 2 421 to facilitate the separation operation of the processed preparation liquid.

[0045] In this embodiment, in order to facilitate the control of the support base 21 and the guide rail 22 to slide along the guide block 24, thereby facilitating the operator to perform operations such as taking and placing the culture container 201 on the operating table 2, a handle is fixedly installed on the front side of the support base 21.

[0046] In this embodiment, in order to facilitate the operator to observe the situation inside the tank body 1, a plurality of observation windows 101 are provided on the tank body 1.

[0047] Working principle: When in use, first turn on the power and start motor three 44. Motor three 44 controls the rotation of hollow shaft 4 through transmission gear 441, and at the same time injects material into the mixing tube through feed pipe 43. Liquid material enters the mixing tank 5 through the upper discharge hole 403. Since the hollow shaft 4 injects material into the mixing tank 5 during the rotation, and the stirring and mixing effect is achieved with the cooperation of multiple stirring rods 501 on the inner wall of the mixing tank 5, the control valve one 41 is opened, so that the prepared liquid after mixing enters the hollow shaft 4 again through the upper feed hole 405 and enters the filter cartridge 61 through the lower discharge hole 404. Since the filter cartridge 61 is fixedly mounted on the hollow shaft 4, the filter cartridge 61 can keep rotating rapidly, thereby realizing the centrifugal operation of the prepared liquid. The prepared liquid after centrifugation enters the hollow shaft 4 again along the inner wall of the sealed tank 6 through the lower feed hole 406 and is transported to the branch pipe 42 position. By controlling different valves two 421, the liquid separation operation into different culture containers 201 is realized.

[0048] The motor 305 is started by operating the control panel. The output shaft of the motor 305 drives the driving gear 306 to rotate. The driving gear 306 meshes with the driven gear 307, thereby driving the screw rod 302 to rotate. Since the two pulleys 303 are connected to the same belt 304, the two screw rods 302 can rotate synchronously, thereby realizing the height adjustment of the hollow ring 3, thereby achieving the purpose of adjusting the height of the tools hung on the hook 32 according to actual needs, so as to facilitate the multi-dimensional robot arm 7 to perform pick-up and placement operations. At the same time, the operator can start the motor 2 311 through the control panel. The output shaft of the motor 2 311 drives the driving gear 312 to rotate. The driving gear 312 meshes with the toothed disc 31, thereby driving the toothed disc 31 to rotate. The rotation of the toothed disc 31 can drive the multiple hooks 32 fixed on its bottom side to adjust their positions, further facilitating the multi-dimensional robot arm 7 to perform pick-up and placement operations on the tools on the hooks 32.

[0049] When the culture container 201 needs to be taken out of the tank body 1, the guide block 24 is controlled by the electric cylinder 23 to move downward, so that the guide rail 22 drives the support seat 21 and the operating table 2 downward synchronously. After the operating table 2 and the culture container 201 are completely located under the tank body 1, the support seat 21 can be pulled by the handle to move the operating table 2 to a position convenient for the operator to take and place the culture container 201.

[0050] The above is a detailed introduction to the CAR-NK cell automated preparation and application platform provided by the present invention. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above examples is only used to help understand the method and core ideas of the present invention. It should be noted that for those skilled in the art, without departing from the principles of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A CAR-NK cell automated preparation and application platform, characterized in that: It comprises a tank body (1), an operating table (2), a lifting adjustment component 1, a hollow ring (3), a lifting adjustment component 2, a rotation adjustment component, a plurality of hooks (32), a hollow shaft (4), a mixing tank (5), a sealing tank (6), a filter cartridge (61), a stirring component, a valve 1 (41), a liquid separation component and a plurality of culture containers (201); The bottom of the tank body (1) is provided with a circular opening, and two support frames (11) are fixedly installed on the bottom of the tank body (1). The lifting and adjusting component 1 is arranged on the two support frames (11) and connected to the tank body (1), and the lifting and adjusting component 1 is adapted to the circular opening. The operating table (2) is arranged on the lifting and adjusting component 1, and the plurality of culture containers (201) are all snap-fitted and installed on the top side of the operating table (2); The hollow ring (3) is slidably mounted on the inner side wall of the tank body (1); the lifting adjustment component 2 is arranged on the tank body (1) and connected to the hollow ring (3); the rotation adjustment component is arranged on the bottom inner wall of the hollow ring (3); and a plurality of hooks (32) are all arranged on the rotation adjustment component; The hollow shaft (4) is rotatably mounted on the top inner wall of the tank body (1), and the top end of the hollow shaft (4) extends to the top of the tank body (1) and is sealed and rotatably mounted with a feed pipe (43). The mixing tank (5) is sealed and rotatably mounted on the hollow shaft (4) and is fixedly connected to the bottom inner wall of the tank body (1), and the stirring assembly is arranged in the mixing tank (5). The sealing tank (6) is fixedly mounted on the bottom of the mixing tank (5) and is sealed and rotatably connected to the hollow shaft (4). The filter cartridge (61) is fixedly mounted on the hollow shaft (4) and is located in the sealing tank (6). The valve 1 (41) is fixedly mounted on the hollow shaft (4) and is located between the mixing tank (5) and the sealing tank (6). The hollow shaft (4) is provided with a There are a plurality of upper discharge holes (403), a plurality of lower discharge holes (404), a plurality of upper feed holes (405) and a plurality of lower feed holes (406); the plurality of upper discharge holes (403) and the plurality of upper feed holes (405) are all located in the mixing tank (5); the plurality of lower feed holes (406) and the plurality of lower discharge holes (404) are respectively located in the sealing tank (6) and the filter cartridge (61); an upper partition (401) and a lower partition (402) are fixedly installed in the hollow shaft (4); the upper partition (401) is located between the plurality of upper discharge holes (403) and the plurality of upper feed holes (405); and the lower partition (402) is located between the plurality of lower feed holes (406) and the plurality of lower discharge holes (404); A housing (102) is fixedly mounted on the top of the tank body (1), a motor (44) is fixedly mounted on the housing (102), and a transmission gear (441) is fixedly sleeved on the output shaft of the motor (44) and the hollow shaft (4), and the two transmission gears (441) are meshed with each other; The liquid separation component is arranged at the bottom end of the hollow shaft (4) and is adapted to the plurality of culture containers (201), and a plurality of multi-dimensional robotic arms (7) are fixedly mounted on the inner side wall of the tank body (1).

2. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The lifting and adjusting component 1 comprises a support base (21), two guide rails (22), two electric cylinders (23) and two guide blocks (24); the guide blocks (24) are slidably installed in the two support frames (11); the guide rails (22) are slidably installed on the sides of the two guide blocks (24) close to each other; the same support base (21) is fixedly installed on the sides of the two guide rails (22) close to each other; the operating table (2) is fixedly installed on the support base (21); the electric cylinders (23) are fixedly installed on both sides of the tank body (1); and the working ends of the two electric cylinders (23) are fixedly connected to the corresponding guide blocks (24).

3. The CAR-NK cell automated preparation and application platform according to claim 2, characterized in that: The cross section of the support seat (21) is convex.

4. The CAR-NK cell automated preparation and application platform according to claim 2, characterized in that: A handle is fixedly mounted on the front side of the support seat (21).

5. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The lifting and adjusting component 2 comprises two inner thread sleeves (301), two screw rods (302), two pulleys (303) and a belt (304). The top of the hollow ring (3) is fixedly mounted with two inner thread sleeves (301). The screw rods (302) are threadedly mounted in the two inner thread sleeves (301). The two screw rods (302) are rotatably connected to the top inner wall of the tank body (1). The top ends of the two screw rods (302) extend to the top of the tank body (1) and are fixedly sleeved with pulleys (303). The two pulleys (303) are connected to the same belt (304) for transmission.

6. The CAR-NK cell automated preparation and application platform according to claim 5, characterized in that: The lifting and adjusting component 2 further comprises a motor 1 (305), a driving gear (306) and a driven gear (307). The motor 1 (305) is fixedly mounted on the top of the housing (102). The output shaft of the motor 1 (305) extends into the housing (102) and is fixedly sleeved with the driving gear (306). The driven gear (307) is fixedly sleeved on the screw rod (302) close to the motor 1 (305). The driving gear (306) and the driven gear (307) are meshed.

7. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The rotation adjustment component comprises a second motor (311), a driving gear (312) and a toothed disc (31); the toothed disc (31) is rotatably mounted on the inner wall of the bottom of the hollow ring (3); the second motor (311) is fixedly mounted on the inner wall of the bottom of the hollow ring (3); the driving gear (312) is fixedly sleeved on the output shaft of the second motor (311); the driving gear (312) is meshed with the toothed disc (31), and a plurality of hooks (32) are fixed on the bottom side of the toothed disc (31).

8. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The stirring assembly comprises a plurality of stirring rods (501), and the plurality of stirring rods (501) are fixedly mounted on the inner side wall of the mixing tank (5), and the plurality of stirring rods (501) are radially arranged with the hollow shaft (4) as the center.

9. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The liquid separation component includes a plurality of branch pipes (42) and a plurality of valves (421). The bottom end of the hollow shaft (4) is fixedly mounted with a plurality of branch pipes (42) in a radial pattern. The ends of the plurality of branch pipes (42) away from the hollow shaft (4) extend to positions directly above the corresponding culture containers (201) and are fixedly mounted with valves (421) respectively.

10. The CAR-NK cell automated preparation and application platform according to claim 1, characterized in that: The tank body (1) is provided with a plurality of observation windows (101).

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