System for efficiently producing 3D cornua cervi pantotrichum stem cell exosome

Abstract

The invention relates to the field of cell culture, and discloses a system for efficiently producing 3D cornu cervi pantotrichum stem cell exosomes, the system comprises a workbench, the left side of the workbench is provided with an ultra-high-speed centrifuge, the upper surface of the workbench is provided with a culture bin, the interior of the culture bin is slidably connected with a heat preservation plate, the outer wall of the heat preservation plate is provided with clamping teeth, and the clamping teeth are arranged on the outer wall of the heat preservation plate. The outer walls of the clamping teeth are arranged in the culture bin, the outer walls of the clamping teeth are fixedly connected with connecting columns, the outer walls of the connecting columns are slidably connected into the heat preservation plate, and reset assemblies are arranged on the outer walls of the connecting columns. A connecting piece moves to be matched with a first supporting plate to stretch a first spring, after the heat preservation plate moves to a proper position, the first spring can release self elasticity, clamping teeth are further bounced into the culture bin, then the heat preservation plate is rapidly fixed, and the effect that the temperature in the culture bin is reduced, and consequently the antler stem cells cannot normally grow is avoided.

Description

Technical Field

[0001] The present invention relates to the field of cell culture technology, and in particular to a system for efficiently producing 3D antler stem cell exosomes. Background Art

[0002] Antler stem cells are a type of cell found in antler tissue that has the potential for self-renewal and multidirectional differentiation. They can continuously produce new stem cells through cell division. Under appropriate conditions, antler stem cells can differentiate into a variety of different cell types, such as osteoblasts, chondrocytes, adipocytes, muscle cells, etc., and then participate in the formation and repair of antler tissue. This characteristic makes antler stem cells have important application value in the fields of tissue engineering and regenerative medicine.

[0003] Existing culture chambers for producing 3D antler stem cell exosomes usually have good sealing and sterility to prevent microbial contamination. However, the culture chambers are not equipped with thermal insulation equipment, which makes the culture temperature in the culture chamber easily affected by the external environment and fluctuates, thereby inhibiting the growth of antler stem cells. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides a system for efficiently producing 3D antler stem cell exosomes, which solves the problem that the existing culture chamber for producing 3D antler stem cell exosomes does not have thermal insulation equipment, resulting in the inhibition of antler stem cell growth.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] A system for efficiently producing 3D antler stem cell exosomes, comprising a workbench, an ultrahigh-speed centrifuge provided on the left side of the workbench, a culture chamber provided on the left side of the workbench, a heat preservation plate slidably connected to the interior of the culture chamber, a latch provided on the outer wall of the heat preservation plate, an outer wall of the latch provided on the interior of the culture chamber, a connecting column fixedly connected to the outer wall of the latch, the outer wall of the connecting column slidably connected to the interior of the heat preservation plate, a reset component provided on the outer wall of the connecting column, the outer wall of the reset component slidably connected to the interior of the heat preservation plate, a support plate 1 provided on the outer wall of the reset component, the outer wall of the connecting column slidably connected to the interior of the support plate 1, and the outer wall of the support plate 1 fixedly connected to the interior of the heat preservation plate.

[0007] By adopting the above technical solution, when the insulation plate moves, it will drive the locking teeth to move, and then drive the connecting column to move. When the connecting column moves, it will drive the reset component to move. When the insulation plate moves to the appropriate position, the reset component will release its own elastic force and bounce the locking teeth into the interior of the culture chamber, thereby fixing the insulation plate inside the culture chamber, improving the overall insulation performance of the culture chamber, and ensuring the normal growth of deer antler stem cells.

[0008] Preferably, the reset assembly includes a connecting plate, the outer wall of the connecting column is fixedly connected to the outer wall of the connecting plate, the outer wall of the connecting plate is provided with a spring 1, the outer wall of the connecting plate is slidably connected to the inside of the insulation board, the outer wall of the spring 1 is provided on the outer wall of the support plate 1, and the outer wall of the spring 1 is provided on the outer wall of the connecting column.

[0009] Preferably, a slideway is provided inside the insulation board, and the latching teeth and the connecting piece are slidably connected inside the insulation board via the slideway.

[0010] Preferably, a groove is provided inside the workbench, and the latch is slidably connected to the inside of the culture chamber through the groove.

[0011] Preferably, the outer wall of the insulation plate is fixedly connected with a tooth plate, and the tooth end of the tooth plate is provided with a transmission assembly, the outer wall of the transmission assembly is rotatably connected to the support frame 2, the upper surface of the support frame 2 is fixedly connected to the support plate 2, the outer wall of the support plate 2 is slidably connected to the inside of the culture chamber, the outer wall of the transmission assembly is rotatably connected to the support plate 3, and the lower surface of the support plate 3 is fixedly connected to the upper surface of the support frame 2, the outer wall of the transmission assembly is threadedly connected to a threaded block, the outer wall of the threaded block is slidably connected to the inside of the support frame 2, the upper surface of the threaded block is fixedly connected to a clamping block, the outer wall of the clamping block is slidably connected to the inside of the support plate 2, the outer wall of the clamping block is fitted with a culture dish, and the lower surface of the culture dish is set on the upper surface of the support plate 2.

[0012] Preferably, the transmission assembly includes a gear, the tooth end of the gear is meshedly connected to the tooth end of the tooth plate, a two-way threaded rod is fixedly connected to the inside of the gear, the outer wall of the two-way threaded rod is rotatably connected to the inside of support frame two and support plate three, and the outer wall of the two-way threaded rod is threadedly connected to the inside of the threaded block.

[0013] Preferably, a second slide groove is provided inside the second support frame, and the threaded block is slidably connected to the inside of the second support frame through the second slide groove.

[0014] Preferably, the outer wall of the support plate 2 is fixedly connected to a transmission column, the outer wall of the transmission column is slidably connected to the support frame 1, the outer wall of the support frame 1 is fixedly connected to the inside of the culture chamber, the outer wall of the transmission column is fixedly connected to a slider, the outer wall of the slider is slidably connected to the outer wall of the support frame 1, the inside of the slider is slidably connected to a rotating plate, the outer wall of the rotating plate is rotatably connected to the inside of the support frame 1, the outer wall of the slider is provided with a spring 2, and the outer wall of the spring 2 is provided on the outer wall of the support frame 1.

[0015] Preferably, a slide groove 1 is provided inside the slider, and the rotating plate is slidably connected to the inside of the slider through the slide groove 1. The culture chamber is used to culture 3D antler stem cell exosomes. The 3D antler stem cell exosomes are modified with miR-21 to promote cell proliferation, migration and angiogenesis during wound healing, inhibit the occurrence of inflammation, reduce fiber formation, and thus promote rapid and scar-free repair of the skin in the early stage of wound healing.

[0016] Preferably, a slide groove three is provided inside the workbench, and the support plate two is slidably connected to the inside of the culture chamber through the slide groove three. The culture chamber is used to culture 3D antler stem cell exosomes. The 3D antler stem cell exosomes are modified with miR-21 to promote cell proliferation, migration and angiogenesis in the wound healing process, inhibit the occurrence of inflammation, reduce fiber formation, and thus promote rapid and scar-free repair of the skin in the early stage of wound healing.

[0017] Working principle: First, slide the insulation plate through the groove into the interior of the culture chamber. When the insulation plate slides inside the culture chamber, it will drive the latch to slide inside the insulation plate, further driving the connecting column to move. When the connecting column moves, it will drive the connecting piece to move. When the connecting piece moves, it will cooperate with the support plate to stretch the spring. When the insulation plate moves to the appropriate position, the spring will release its own elastic force and bounce the latch into the interior of the culture chamber, thereby quickly fixing the insulation plate and preventing the deer antler stem cells from growing abnormally due to the temperature drop in the culture chamber.

[0018] When the insulation plate moves, it drives the tooth plate to move, which in turn drives the gear to rotate. When the gear rotates, it drives the bidirectional threaded rod to rotate, which in turn drives the threaded block to move. When the threaded block moves, it drives the clamping block to move. By moving the clamping block, the culture dish is clamped and fixed, preventing the culture medium and antler stem cells in the culture dish from spilling due to external factors.

[0019] When the support plate moves, it will drive the transmission column to move, and further drive the slider to move. When the slider moves, it will cooperate with the support frame 1 to compress or release the spring 2. When the slider moves, it will also drive the rotating plate to rotate. By rotating the rotating plate, the slider is limited, and the effect of preventing the support plate 2 from falling off from the inside of the culture chamber due to excessive force when taking out the support plate 2 is further achieved.

[0020] The present invention provides a system for efficiently producing 3D antler stem cell exosomes. It has the following beneficial effects:

[0021] 1. The present invention drives the latch to move by sliding the insulation plate, and further drives the connecting column to move. The connecting piece will move under the drive of the connecting column. While the connecting piece moves, it will also cooperate with the support plate to stretch the spring. When the insulation plate moves to a suitable position, the spring will release its own elastic force, further bounce the latch into the interior of the culture chamber, and then quickly fix the insulation plate, avoiding the temperature in the culture chamber from decreasing, which will cause the antler stem cells to be unable to grow normally.

[0022] 2. The present invention drives the tooth plate to move by moving the insulation plate, and further drives the gear to rotate. When the gear rotates, it will drive the bidirectional threaded rod to rotate synchronously, and further drive the threaded block to move. The clamping block will move under the drive of the threaded block. Through the movement of the clamping block, the culture dish can be clamped and fixed, avoiding the liquid in the culture dish from spilling due to external force, which will cause the antler stem cell culture work to be unable to proceed normally.

[0023] 3. The present invention drives the transmission column to move through the support plate 2, and further drives the slider to move. When the slider moves, it will drive the rotating plate to rotate. The spring 2 is used to reset the slider. Through the rotation of the rotating plate, the slider is limited, and the support plate 2 is further limited, thereby preventing the support plate 2 from falling off due to excessive force when taking out the support plate 2. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Schematic diagram of isolated and extracted ASCs;

[0025] Figure 2 Schematic diagram of the comparison of ASC-Exos regulation of angiogenesis in tube formation assay;

[0026] Figure 3 Schematic diagram of angiogenesis regulation by miR-21-modified ASC-Exos;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure of the present invention;

[0028] Figure 5 Schematic diagram of three partial structures of the chute of the present invention;

[0029] Figure 6 It is a schematic diagram of the local structure of the slideway of the present invention;

[0030] Figure 7 It is a schematic diagram of the local structure of the culture dish of the present invention;

[0031] Figure 8 It is a schematic diagram of the local structure of the rotating plate of the present invention;

[0032] Figure 9 It is a schematic diagram of the local structure of the tooth plate of the present invention;

[0033] Figure 10 It is a schematic diagram of the local structure of the gear of the present invention.

[0034] Among them, 1. workbench; 2. ultra-high-speed centrifuge; 3. culture chamber; 4. groove; 5. insulation plate; 6. latch; 7. connecting column; 8. connecting piece; 9. support plate 1; 10. spring 1; 11. slide; 12. support plate 2; 13. transmission column; 14. slider; 15. support frame 1; 16. rotating plate; 17. spring 2; 18. slide 1; 19. tooth plate; 20. gear; 21. two-way threaded rod; 22. support frame 2; 23. threaded block; 24. clamping block; 25. support plate 3; 26. slide 2; 27. culture dish; 28. slide 3. DETAILED DESCRIPTION

[0035] The following will clearly and completely describe the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] Please see the attached Figure 1 -Attached Figure 8 The embodiment of the present invention provides a system for efficiently producing 3D antler stem cell exosomes, comprising a workbench 1, an ultrahigh-speed centrifuge 2 being provided on the left side of the workbench 1, a culture chamber 3 being provided on the upper surface of the workbench 1, a heat preservation plate 5 being slidably connected to the interior of the culture chamber 3, an outer wall of the heat preservation plate 5 being provided with a latch 6, an outer wall of the latch 6 being provided inside the culture chamber 3, a connecting column 7 being fixedly connected to the outer wall of the latch 6, an outer wall of the connecting column 7 being slidably connected to the interior of the heat preservation plate 5, an outer wall of the connecting column 7 being provided with a reset component, an outer wall of the reset component being slidably connected to the interior of the heat preservation plate 5, an outer wall of the reset component being provided with a support plate 9, an outer wall of the connecting column 7 being slidably connected to the interior of the support plate 9, and an outer wall of the support plate 9 being fixedly connected to the interior of the heat preservation plate 5;

[0037] Specifically, the workbench 1 is used to support the culture chamber 3, the ultra-high-speed centrifuge 2 is used to further separate protein aggregates, lipoproteins, etc., so as to obtain 3D antler stem cell exosomes with higher purity, and the culture chamber 3 is used to provide a suitable temperature for the growth of antler stem cells and exosome secretion, and push the insulation plate 5 to slide inside the culture chamber 3, further driving the latch 6 to move. When the latch 6 moves, the connecting column 7 is driven to move by the fixing effect of the latch 6 and the connecting column 7, and further driving the reset component to move. The support plate 9 is used to support the movement of the connecting column 7. When the latch 6 moves to the appropriate position, the reset component releases its own elastic force, and then bounces the latch 6 into the interior of the culture chamber 3, and the movement of the latch 6 is realized. The insulation plate 5 is quickly installed to prevent the temperature inside the culture chamber 3 from escaping and affecting the normal growth of antler stem cells. MiR-21 is introduced into antler stem cells (ASCs) through methods such as lentiviral transfection, and then the miR-21-modified antler stem cells are inoculated into the 3D culture system. After the production of miR-21-modified 3D antler stem cell exosomes (3D-ASC-Exos) is completed, they can be further separated and purified by the ultrahigh-speed centrifuge 2 to remove impurities and improve the purity and quality of the exosomes. When 3D-ASC-Exos are produced on a large scale, antler stem cells can be inoculated in multiple devices at the same time, and the stable environment and efficient operation of the device can be used to achieve batch production of exosomes.miR-21 modified ASC-Exos can promote skin repair through multiple pathways. On the one hand, miR-21 has the function of regulating cell proliferation, migration and apoptosis. On the other hand, it can promote the proliferation of skin cells and accelerate cell renewal during wound healing. After skin damage, it will target key genes, reduce cell apoptosis by inhibiting the expression of pro-apoptotic proteins, and promote the production of anti-apoptotic proteins, enhance cell survival ability, and create favorable conditions for cell proliferation. miR-21 modified ASC-Exos can also activate cell cycle-related proteins, drive the cell cycle to progress smoothly from one stage to the next, accelerate the division and proliferation of skin cells, and promote cells to pass through the G1 phase and enter the S phase faster by upregulating the expression of key proteins such as cyclin-dependent kinases (CDKs), increase the number of cells, accelerate cell renewal during wound healing, and promote the regeneration of skin tissue. Skin damage is often accompanied by inflammatory response, and miR-21 modified ASC-Exos can accurately regulate this process. It can inhibit Overexpression of inflammatory factors. When the skin is damaged, inflammatory cells will rapidly aggregate and release a large number of inflammatory factors. miR-21-modified ASC-Exos can interact with inflammatory-related genes, inhibit the production and release of these inflammatory factors, thereby effectively reducing the intensity of the inflammatory response. It can also regulate the activity and function of immune cells, promote the polarization of immune cells, and induce the transformation of macrophages from the pro-inflammatory M1 type to the anti-inflammatory M2 type. M2 macrophages can secrete anti-inflammatory factors, promote tissue repair and regeneration, and create a favorable microenvironment for skin damage repair. In addition, miR-21 can enhance the survival ability of endothelial cells by inhibiting pro-apoptotic proteins (such as PTEN and PDCD4) and upregulating pro-survival signals (such as the PI3K / Akt pathway), reducing endothelial cell apoptosis, and providing a stable cellular basis for angiogenesis. By inhibiting PTEN (a target gene of miR-21), it activates the Akt / eNOS pathway and increases NO release, thereby promoting vasodilation and neovascularization of HUVECs.

[0038] Please see the attached Figure 1 -Attached Figure 8 , the reset assembly includes a connecting piece 8, the outer wall of the connecting column 7 is fixedly connected to the outer wall of the connecting piece 8, the outer wall of the connecting piece 8 is provided with a spring 10, the outer wall of the connecting piece 8 is slidably connected to the inside of the insulation board 5, the outer wall of the spring 10 is provided on the outer wall of the support plate 9, and the outer wall of the spring 10 is provided on the outer wall of the connecting column 7;

[0039] Specifically, when the connecting column 7 moves, the connecting piece 8 will be driven to move through the fixing effect of the connecting column 7 and the connecting piece 8. When the connecting piece 8 moves, it will cooperate with the support plate 9 to achieve the effect of stretching the spring.

[0040] Please see the attached Figure 1 -Attached Figure 6 , a slideway 11 is provided inside the insulation board 5, and the latch 6 and the connecting piece 8 are slidably connected to the inside of the insulation board 5 through the slideway 11;

[0041] Specifically, the slideway 11 is used to limit the movement trajectory of the latching teeth 6 and the connecting piece 8, thereby improving the stability of the latching teeth 6 and the connecting piece 8 when they move.

[0042] Please see the attached Figure 4 -Attached Figure 10 A groove 4 is provided inside the workbench 1, and the latch 6 is slidably connected to the inside of the culture chamber 3 through the groove 4;

[0043] Specifically, the special inclined surface design of the groove 4 and the latching tooth 6 can improve the smoothness of the latching tooth 6 when sliding.

[0044] Please see the attached Figure 1 -Attached Figure 7 The outer wall of the insulation plate 5 is fixedly connected to a tooth plate 19, and the tooth end of the tooth plate 19 is provided with a transmission assembly. The outer wall of the transmission assembly is rotatably connected to a support frame 22, and the upper surface of the support frame 22 is fixedly connected to the support plate 2 12. The outer wall of the support plate 2 12 is slidably connected to the inside of the culture bin 3. The outer wall of the transmission assembly is rotatably connected to a support plate 3 25, and the lower surface of the support plate 3 25 is fixedly connected to the upper surface of the support frame 2 22. The outer wall of the transmission assembly is threadedly connected to a threaded block 23, and the outer wall of the threaded block 23 is slidably connected to the inside of the support frame 22. The upper surface of the threaded block 23 is fixedly connected to a clamping block 24, and the outer wall of the clamping block 24 is slidably connected to the inside of the support plate 2 12. The outer wall of the clamping block 24 is fitted with a culture dish 27, and the lower surface of the culture dish 27 is provided on the upper surface of the support plate 2 12;

[0045] Specifically, when the insulation plate 5 moves, the tooth plate 19 will be driven to move through the fixing effect of the insulation plate 5 and the tooth plate 19, and when the tooth plate 19 moves, the transmission component will be driven to rotate. The support frame 22 and the support plate 3 25 are used to support the rotation of the transmission component. When the transmission component rotates, the threaded block 23 will be driven to move. When the threaded block 23 moves, the fixing effect of the threaded block 23 and the clamping block 24 will drive the clamping block 24 to move, thereby achieving the effect of clamping and fixing the culture dish 27, ensuring that the culture dish remains stable during the culture process, providing a stable growth environment for antler stem cells, which is conducive to the efficient production of 3D antler stem cell exosomes (3D-ASC-Exos), and can ensure that its biological activity can be better maintained after miR-21 modification, ensuring that miR-21-modified ASC-Exos have sufficient and high-quality sources for skin injury repair research and treatment.

[0046] Please see the attached Figure 4 -Attached Figure 10 The transmission assembly includes a gear 20, the tooth end of the gear 20 is meshedly connected to the tooth end of the gear plate 19, a bidirectional threaded rod 21 is fixedly connected to the interior of the gear 20, the outer wall of the bidirectional threaded rod 21 is rotatably connected to the interior of the support frame 22 and the support plate 3 25, and the outer wall of the bidirectional threaded rod 21 is threadedly connected to the interior of the threaded block 23;

[0047] Specifically, when the tooth plate 19 moves, it will drive the gear 20 to rotate. When the gear 20 rotates, the fixing effect of the gear 20 and the bidirectional threaded rod 21 will drive the bidirectional threaded rod 21 to rotate inside the support frame 22 and the support plate 3 25. Through the rotation of the bidirectional threaded rod 21, the threaded block 23 is driven to move in the opposite direction.

[0048] Please see the attached Figure 7 , a second slide groove 26 is provided inside the second support frame 22, and the threaded block 23 is slidably connected to the inside of the second support frame 22 through the second slide groove 26;

[0049] Specifically, the second slide groove 26 is used to limit the running track of the threaded block 23, thereby improving the stability of the threaded block 23 during operation.

[0050] Please see the attached Figure 1 -Attached Figure 6 , the outer wall of the support plate 12 is fixedly connected to the transmission column 13, the outer wall of the transmission column 13 is slidably connected to the support frame 15, the outer wall of the support frame 15 is fixedly connected to the inside of the culture chamber 3, the outer wall of the transmission column 13 is fixedly connected to the slider 14, the outer wall of the slider 14 is slidably connected to the outer wall of the support frame 15, the inside of the slider 14 is slidably connected to the rotating plate 16, the outer wall of the rotating plate 16 is rotatably connected to the inside of the support frame 15, the outer wall of the slider 14 is provided with a spring 2 17, and the outer wall of the spring 2 17 is provided on the outer wall of the support frame 15;

[0051] Specifically, when the support plate 2 12 moves, the fixing effect of the support plate 2 12 and the transmission column 13 will drive the transmission column 13 to slide inside the support frame 15. When the transmission column 13 slides, it will drive the slider 14 to move, and further drive the rotating plate 16 to rotate. The spring 2 17 is used to reset the slider 14. Through the mutual cooperation between the slider 14, the rotating plate 16, and the spring 2 17, the sliding distance of the support plate 2 12 when the support plate 2 12 is taken out is limited, thereby avoiding the effect of the support plate 2 12 sliding out of the culture chamber 3 due to operational errors.

[0052] Please see the attached Figure 1 -Attached Figure 10 A slide groove 18 is provided inside the slider 14, and the rotating plate 16 is slidably connected to the inside of the slider 14 through the slide groove 18;

[0053] Specifically, the sliding groove 18 is used to limit the sliding track of the rotating plate 16, thereby achieving the effect of limiting the sliding block 14.

[0054] Please see the attached Figure 1 -Attached Figure 7 A third slide groove 28 is provided inside the workbench 1, and the second support plate 12 is slidably connected to the interior of the culture chamber 3 through the third slide groove 28. The culture chamber 3 is used to culture 3D antler stem cell exosomes. The 3D antler stem cell exosomes are modified with miR-21 and used for skin damage repair.

[0055] Specifically, the third slide groove 28 is used to limit the sliding track of the second support plate 12, thereby further improving the stability of the second support plate 12 when the second support plate 12 is taken out or placed.

[0056] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A system for efficiently producing 3D antler stem cell exosomes, comprising a workbench (1), characterized in that: An ultra-high-speed centrifuge (2) is provided on the left side of the workbench (1), and a culture chamber (3) is provided on the upper surface of the workbench (1). The interior of the culture chamber (3) is slidably connected to a heat preservation plate (5), and the outer wall of the heat preservation plate (5) is provided with a latch (6). The outer wall of the latch (6) is provided inside the culture chamber (3), and the outer wall of the latch (6) is fixedly connected to a connecting column (7). The outer wall of the connecting column (7) is slidably connected to the interior of the heat preservation plate (5), and the outer wall of the connecting column (7) is provided with a reset component. The outer wall of the reset component is slidably connected to the interior of the heat preservation plate (5), and the outer wall of the reset component is provided with a support plate (9). The outer wall of the connecting column (7) is slidably connected to the interior of the support plate (9), and the outer wall of the support plate (9) is fixedly connected to the interior of the heat preservation plate (5).

2. The system for efficiently producing 3D antler stem cell exosomes according to claim 1, characterized in that: The reset assembly includes a connecting piece (8), the outer wall of the connecting column (7) is fixedly connected to the outer wall of the connecting piece (8), the outer wall of the connecting piece (8) is provided with a spring (10), the outer wall of the connecting piece (8) is slidably connected to the inside of the insulation board (5), the outer wall of the spring (10) is provided on the outer wall of the support plate (9), and the outer wall of the spring (10) is provided on the outer wall of the connecting column (7).

3. The system for efficiently producing 3D antler stem cell exosomes according to claim 2, characterized in that: A slideway (11) is provided inside the heat preservation plate (5), and the latching teeth (6) and the connecting piece (8) are slidably connected inside the heat preservation plate (5) via the slideway (11).

4. The system for efficiently producing 3D antler stem cell exosomes according to claim 1, characterized in that: A groove (4) is provided inside the workbench (1), and the latching teeth (6) are slidably connected to the inside of the culture chamber (3) through the groove (4).

5. The system for efficiently producing 3D antler stem cell exosomes according to claim 1, characterized in that: The outer wall of the insulation plate (5) is fixedly connected to a tooth plate (19), and a transmission assembly is provided at the tooth end of the tooth plate (19). The outer wall of the transmission assembly is rotatably connected to a support frame 2 (22), and the upper surface of the support frame 2 (22) is fixedly connected to a support plate 2 (12). The outer wall of the support plate 2 (12) is slidably connected to the inside of the culture chamber (3). The outer wall of the transmission assembly is rotatably connected to a support plate 3 (25), and the lower surface of the support plate 3 (25) is fixedly connected to the support frame 2. The upper surface of the support frame (22) is connected to the outer wall of the transmission assembly by a threaded block (23), and the outer wall of the threaded block (23) is slidably connected to the inside of the support frame (22). The upper surface of the threaded block (23) is fixedly connected to the clamping block (24), and the outer wall of the clamping block (24) is slidably connected to the inside of the support plate (12). The outer wall of the clamping block (24) is fitted with a culture dish (27), and the lower surface of the culture dish (27) is arranged on the upper surface of the support plate (12).

6. The system for efficiently producing 3D antler stem cell exosomes according to claim 5, characterized in that: The transmission assembly includes a gear (20), the tooth end of the gear (20) is meshedly connected to the tooth end of the tooth plate (19), the interior of the gear (20) is fixedly connected with a bidirectional threaded rod (21), the outer wall of the bidirectional threaded rod (21) is rotatably connected to the interior of the support frame 2 (22) and the support plate 3 (25), and the outer wall of the bidirectional threaded rod (21) is threadedly connected to the interior of the threaded block (23).

7. The system for efficiently producing 3D antler stem cell exosomes according to claim 5, characterized in that: A second slide groove (26) is provided inside the second support frame (22), and the threaded block (23) is slidably connected to the inside of the second support frame (22) through the second slide groove (26).

8. The system for efficiently producing 3D antler stem cell exosomes according to claim 5, characterized in that: The outer wall of the support plate 2 (12) is fixedly connected to a transmission column (13), the outer wall of the transmission column (13) is slidably connected to a support frame 1 (15), the outer wall of the support frame 1 (15) is fixedly connected to the inside of the culture chamber (3), the outer wall of the transmission column (13) is fixedly connected to a slider (14), the outer wall of the slider (14) is slidably connected to the outer wall of the support frame 1 (15), the inside of the slider (14) is slidably connected to a rotating plate (16), the outer wall of the rotating plate (16) is rotatably connected to the inside of the support frame 1 (15), the outer wall of the slider (14) is provided with a spring 2 (17), the outer wall of the spring 2 (17) is provided on the outer wall of the support frame 1 (15).

9. The system for efficiently producing 3D antler stem cell exosomes according to claim 8, characterized in that: A sliding groove (18) is provided inside the slider (14), and the rotating plate (16) is slidably connected to the inside of the slider (14) through the sliding groove (18).

10. The system for efficiently producing 3D antler stem cell exosomes according to claim 5, characterized in that: The workbench (1) is provided with a third slide groove (28) inside, and the second support plate (12) is slidably connected to the inside of the culture chamber (3) through the third slide groove (28). The culture chamber (3) is used to culture 3D antler stem cell exosomes. The 3D antler stem cell exosomes are modified with miR-21 to promote cell proliferation, migration and angiogenesis during wound healing, inhibit the occurrence of inflammation, reduce fiber formation, and thus promote rapid and scar-free repair of the skin in the early stage of wound healing.

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