Nutritional health-care product for regulating immune function and preparation method of nutritional health-care product

Abstract

The invention relates to the technical field of traditional Chinese medicine health-care products, in particular to a nutritional health-care product for regulating an immune function and a preparation method thereof. The nutritional health-care product capable of regulating the immune function comprises the following components in parts by weight: 5-15 parts of velvet antler mushroom polysaccharide and / or 5-15 parts of gloeostereum incarnatum polysaccharide, 1-10 parts of echinacea purpurea, 5-15 parts of chia seeds, 5-15 parts of spirulina, 1-10 parts of calabash shells, 1-10 parts of nigella glandulifera seed oil, 0.1-5 parts of melatonin, 1-10 parts of pea seedlings, 0.1-5 parts of magnesium L-threonate, 1-10 parts of black garlic polysaccharide, 1-10 parts of peanut meal polysaccharide, 0.1-8 parts of pumpkin seeds and 0.1-3 parts of acetylated oxidized starch. 0.1 to 5 parts of animal bifidobacterium and 0.1 to 5 parts of streptococcus thermophilus. According to the invention, immunity is regulated through a synergistic effect, and immune regulation and anti-inflammation are synergistic; constructing an anti-oxidation network; regulating intestinal health and flora; the metabolism is regulated and controlled; the aging is resisted. Through the synergistic effect of the effective components, the immunity is further enhanced, and no side effect exists.

Description

Technical Field

[0001] The present application relates to the technical field of traditional Chinese medicine health care products, and in particular to a nutritional health care product for regulating immune function and a preparation method thereof. Background Art

[0002] Immunity is the body's own defense mechanism. It is the body's ability to identify and eliminate any invading foreign bodies (viruses, bacteria, etc.), to deal with aging, damaged, dead, and degenerated cells, and to identify and deal with mutated and virus-infected cells within the body. Modern immunology considers immunity to be the body's physiological response to identify and eliminate "foreign" entities. The immune system is the body responsible for this function.

[0003] A weakened immune system is more susceptible to infections and cancer. Various factors can prevent the immune system from functioning properly, making it highly susceptible to bacterial, viral, and fungal infections. Therefore, the most direct manifestation of a weakened immune system is susceptibility to illness. Frequent illnesses increase the strain on the body, leading to symptoms such as weakness, malnutrition, lethargy, fatigue, decreased appetite, and sleep disturbances. Sickness and medication become commonplace. Recovery from each illness can be lengthy, and recurring. This long-term decline can lead to physical and intellectual impairment and can easily lead to serious illness. Therefore, boosting immunity and reducing illness is even more important than improving medical care.

[0004] Currently, people strengthen their immune systems by consuming various immune-boosting foods, such as dairy products, fish, and spirulina. However, this approach is slow to take effect, and there are no clear medical guidelines on the appropriate dosage and method of intake. Medication is more effective in boosting immunity. Western medicine primarily aims to enhance immunity by providing the body with various vitamins and minerals. However, this approach doesn't address the root cause of low immunity, and excessive intake of minerals and vitamins poses potential risks. Traditional Chinese Medicine (TCM) offers a more fundamental approach to boosting immunity, but the current market for TCM health supplements is mixed, with few truly effective and harmless products.

[0005] Therefore, it is of great significance to develop a safe, directly edible product that regulates immune function and is composed of active ingredients from traditional medicinal and edible products or natural plants. Summary of the Invention

[0006] The purpose of this application is to provide a nutritional health product for regulating immune function and a preparation method thereof, so as to solve or alleviate the problems existing in the above-mentioned prior art.

[0007] In order to achieve the above objectives, this application provides the following technical solutions:

[0008] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0009] 5-15 parts of velvet antler polysaccharide and / or 5-15 parts of elm ear polysaccharide, 1-10 parts of Echinacea chinensis, 5-15 parts of chia seeds, 5-15 parts of spirulina, 1-10 parts of cucurbit shell, 1-10 parts of black cumin seed oil, 0.1-5 parts of melatonin, 1-10 parts of pea sprouts, 0.1-5 parts of magnesium L-threonate, 1-10 parts of black garlic polysaccharide, 1-10 parts of peanut meal polysaccharide, 0.1-8 parts of pumpkin seeds, 0.1-3 parts of acetylated oxidized starch, 0.1-5 parts of animal bifidobacterium, and 0.1-5 parts of thermophilic streptococcus.

[0010] Preferably, it also includes 0.1 to 5 parts of oligofructose-nanoselenium.

[0011] Further preferably, the oligofructose-nanoselenium is prepared by the following steps:

[0012] At room temperature, oligofructose is dissolved in an ethanol aqueous solution. After the oligofructose is fully dissolved, sodium selenite is added. Then, the solution is reacted in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, the reaction liquid is centrifuged at a speed of 10000 r / min. The precipitate is washed with water three times and then centrifuged at a speed of 10000 r / min. The precipitate is dried and ground to obtain oligofructose-nanoselenium.

[0013] More preferably, the ratio of the oligofructose to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, the mass ratio of the oligofructose to sodium selenite is 2-5:1, and the oligofructose is selected from kestose.

[0014] Preferably, 0.1 to 5 parts of zinc-coordinated DHA-nanoselenium are also included.

[0015] Further preferably, the zinc-coordinated DHA-nanoselenium is prepared by the following steps:

[0016] (1) Dissolve galactoligosaccharide in an ethanol aqueous solution at room temperature. After the galactoligosaccharide is fully dissolved, add sodium selenite. Then, react the solution in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, set the reaction solution aside.

[0017] (2) Add DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

[0018] More preferably, in step (1), the ratio of the galactoligosaccharide to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, and the mass ratio of the fructooligosaccharide to sodium selenite is 2-5:1.

[0019] More preferably, in step (2), the mass ratio of the galacto-oligosaccharide to DHA and zinc nitrate is 2:1:0.9-1.

[0020] Preferably, the pharmaceutical excipient is further included, and the pharmaceutical excipient is one or more of resistant dextrin, microcrystalline cellulose, and magnesium stearate.

[0021] A method for preparing a nutritional health product for regulating immune function comprises the following steps:

[0022] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0023] Beneficial effects:

[0024] The present invention is based on velvet mushroom polysaccharide, elm ear polysaccharide, Echinacea chinensis, chia seeds, spirulina, gourd shell, black seed oil, melatonin, pea seedlings, L-threonate magnesium, black garlic polysaccharide, peanut meal polysaccharide, pumpkin seeds, acetylated oxidized starch, animal bifidobacterium, thermophilic streptococcus, oligofructose-nano selenium and coordinated DHA-nano selenium as raw materials, wherein, the active ingredients of velvet mushroom polysaccharide are beta-glucan and acidic polysaccharide, with the functions of regulating immunity, anti-oxidation, etc., at the same time, it can promote the proliferation of probiotic animal bifidobacterium, inhibit the growth of pathogenic bacteria, repair intestinal barrier function, and alleviate intestinal damage. The core active substances of elm ear polysaccharide are beta-glucan and Hericium erinaceus polysaccharide, with the functions of immunomodulation, antibacterial, anti-oxidation, and intestinal barrier repair. On the one hand, velvet mushroom polysaccharide can enhance innate immune response, while elm ear polysaccharide regulates adaptive immunity and suppresses inflammation, and combined use can achieve immune balance (such as alleviating excessive inflammation in autoimmune diseases, etc.). On the other hand, the strong antioxidant ability of elm ear polysaccharides protects intestinal cells, and pilose antler polysaccharides promote the proliferation of probiotics. Their combined use can improve intestinal microecology and enhance overall antioxidant defense.

[0025] The active ingredients in Echinacea are chicoric acid, echinacea polyphenols, and caffeic acid. Chicoric acid exhibits multiple biological activities, including boosting the immune system and combating infection and inflammation. Echinacea polyphenols exhibit antibacterial and anti-inflammatory properties, enhancing immunity, and increasing cell viability. Caffeic acid can reduce chronic inflammation by inhibiting the ROS / MAPK / NF-κB signaling pathway. Cucurbitacin husks contain cucurbitacin E, cucurbitacins, and cucurbitacins, saponins, citrulline, vitamins, and trace elements. Cucurbitacin E exhibits anti-inflammatory and antioxidant properties, while cucurbitacins and cucurbitacins possess strong antioxidant and anti-inflammatory effects. The main active ingredients in black cumin seed oil include thymoquinone, thymohydroquinone, and thymol. Thymoquinone exhibits multiple antioxidant, antibacterial, anti-inflammatory, and immunomodulatory effects. Melatonin (N-acetyl-5-methoxytryptamine) can regulate immunity and scavenge free radicals. Black garlic polysaccharides exhibit antioxidant and prebiotic properties. Peanut meal polysaccharides exhibit anti-inflammatory properties. The active substances in Echinacea, gourd shell, black cumin seed oil, melatonin, black garlic polysaccharide and peanut meal polysaccharide include polysaccharides, flavonoids, linoleic acid and other active substances. Peanut meal polysaccharide, Echinacea, gourd shell and melatonin have synergistic anti-inflammatory effects, black cumin seed oil, melatonin and black garlic polysaccharide have antioxidant synergistic effects, and Echinacea polysaccharide, peanut meal polysaccharide and black garlic polysaccharide synergistically enhance immunity and improve immune cell activity (such as the phagocytic ability of macrophages is increased by up to 40%).

[0026] Magnesium L-threonate can inhibit the secretion of TNF-α, a pro-inflammatory cytokine associated with many inflammatory diseases. Pea sprouts contain vitamin C, vitamin K, isoflavones, polyphenols, and flavonoids, providing active substances such as biotin. Oligofructose-nanoselenium provides the trace element selenium, while zinc-coordinated DHA-nanoselenium provides the trace elements selenium, zinc, and DHA. Acetylated oxidized starch, oligofructose-nanoselenium, and zinc-coordinated DHA-nanoselenium provide a variety of prebiotics, while animal Bifidobacterium and thermophilic Streptococcus provide probiotics. Animal Bifidobacterium can improve constipation and enhance immunity, while thermophilic Streptococcus can promote digestion and absorption.

[0027] The present invention regulates immunity through the synergistic effect between the raw materials: (1) immune regulation and anti-inflammatory synergy, antler antler polysaccharide and elm ear polysaccharide synergize, Echinacea chinensis and black seed oil synergize, and reduce inflammatory factors through dual synergistic effect; (2) antioxidant network construction, melatonin, black garlic polysaccharide and gourd shell synergize, spirulina and chia seeds synergize, and further eliminate free radicals through dual synergistic effect; (3) intestinal health and flora regulation, animal bifidobacterium, thermophilic streptococcus and oligofructose-nanoselenium synergize, pea sprouts and pumpkin seeds synergize, and achieve intestinal health through flora regulation; (4) metabolic regulation, chia seeds and peanut meal polysaccharide synergize, L-magnesium threonate and melatonin synergize, regulate metabolism and anti-aging. The present invention further enhances immunity through the synergistic effect between the active ingredients without side effects. DETAILED DESCRIPTION

[0028] The present application will be described in detail below. The various examples are provided by way of explanation of the present application and do not limit the present application. In fact, it will be clear to those skilled in the art that modifications and variations can be made in the present application without departing from the scope or spirit of the present application. For example, a feature shown or described as part of one embodiment can be used in another embodiment to produce yet another embodiment. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention should fall within the scope of protection of the embodiments of the present invention.

[0029] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0030] 5-15 parts of velvet antler polysaccharide and / or 5-15 parts of elm ear polysaccharide, 1-10 parts of Echinacea chinensis, 5-15 parts of chia seeds, 5-15 parts of spirulina, 1-10 parts of cucurbit shell, 1-10 parts of black cumin seed oil, 0.1-5 parts of melatonin, 1-10 parts of pea sprouts, 0.1-5 parts of magnesium L-threonate, 1-10 parts of black garlic polysaccharide, 1-10 parts of peanut meal polysaccharide, 0.1-8 parts of pumpkin seeds, 0.1-3 parts of acetylated oxidized starch, 0.1-5 parts of animal bifidobacterium, and 0.1-5 parts of thermophilic streptococcus.

[0031] Preferably, it also includes 0.1 to 5 parts of oligofructose-nanoselenium.

[0032] Further preferably, the oligofructose-nanoselenium is prepared by the following steps:

[0033] At room temperature, oligofructose is dissolved in an ethanol aqueous solution. After the oligofructose is fully dissolved, sodium selenite is added. Then, the solution is reacted in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, the reaction liquid is centrifuged at a speed of 10000 r / min. The precipitate is washed with water three times and then centrifuged at a speed of 10000 r / min. The precipitate is dried and ground to obtain oligofructose-nanoselenium.

[0034] More preferably, the ratio of the oligofructose to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, the mass ratio of the oligofructose to sodium selenite is 2-5:1, and the oligofructose is selected from kestose.

[0035] Preferably, 0.1 to 5 parts of zinc-coordinated DHA-nanoselenium are also included.

[0036] Further preferably, the zinc-coordinated DHA-nanoselenium is prepared by the following steps:

[0037] (1) Dissolve galactoligosaccharide in an ethanol aqueous solution at room temperature. After the galactoligosaccharide is fully dissolved, add sodium selenite. Then, react the solution in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, set the reaction solution aside.

[0038] (2) Add DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

[0039] More preferably, in step (1), the ratio of the galactoligosaccharide to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, and the mass ratio of the fructooligosaccharide to sodium selenite is 2-5:1.

[0040] More preferably, in step (2), the mass ratio of the galacto-oligosaccharide to DHA and zinc nitrate is 2:1:0.9-1.

[0041] Preferably, the pharmaceutical excipient is further included, and the pharmaceutical excipient is one or more of resistant dextrin, microcrystalline cellulose, and magnesium stearate.

[0042] A method for preparing a nutritional health product for regulating immune function comprises the following steps:

[0043] The above ingredients are weighed separately, and Echinacea, chia seeds, spirulina, gourd shells, nigella sativa seed oil, pea sprouts, and pumpkin seeds are ground into an ultrafine powder with a particle size of 200 mesh or larger. The remaining ingredients are added to the ultrafine powder, mixed evenly, and then granulated, dried, and sterilized to obtain the product. Raw material sources: Pyrola antler polysaccharide, elm ear polysaccharide, melatonin, magnesium L-threonate, black garlic polysaccharide, and peanut meal polysaccharide are all food grade and commercially available. Echinacea, chia seeds, spirulina, gourd shells, nigella sativa seed oil, pea sprouts, and pumpkin seeds are all washed and sterilized before use.

[0044] Example 1

[0045] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0046] 10 parts of velvet antler polysaccharide, 2 parts of echinacea, 5 parts of chia seeds, 5 parts of spirulina, 2 parts of gourd shell, 2 parts of black cumin seed oil, 0.5 parts of melatonin, 1 part of pea sprouts, 0.15 parts of magnesium L-threonate, 2 parts of black garlic polysaccharide, 1.5 parts of peanut meal polysaccharide, 3 parts of pumpkin seeds, 0.5 parts of acetylated oxidized starch, 0.5 parts of animal bifidobacterium, 0.5 parts of streptococcus thermophilus, 1 part of resistant dextrin, 1 part of microcrystalline cellulose, and 1 part of magnesium stearate.

[0047] The preparation method thereof comprises the following steps:

[0048] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0049] Example 2

[0050] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0051] 15 parts of elm ear polysaccharide, 7 parts of Echinacea chinensis, 8 parts of chia seeds, 10 parts of spirulina, 5 parts of gourd shell, 6 parts of black cumin seed oil, 3 parts of melatonin, 5 parts of pea sprouts, 3 parts of magnesium L-threonate, 6 parts of black garlic polysaccharide, 6 parts of peanut meal polysaccharide, 8 parts of pumpkin seeds, 2 parts of acetylated oxidized starch, 5 parts of animal Bifidobacterium, 3 parts of Streptococcus thermophilus, 2 parts of resistant dextrin, 3 parts of microcrystalline cellulose, and 3 parts of magnesium stearate.

[0052] The preparation method thereof comprises the following steps:

[0053] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0054] Example 3

[0055] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0056] 8 parts of velvet antler polysaccharide, 5 parts of elm ear polysaccharide, 2 parts of echinacea, 5 parts of chia seeds, 5 parts of spirulina, 2 parts of gourd shell, 2 parts of black cumin seed oil, 0.5 parts of melatonin, 1 part of pea sprouts, 0.15 parts of magnesium L-threonate, 2 parts of black garlic polysaccharide, 1.5 parts of peanut meal polysaccharide, 3 parts of pumpkin seeds, 0.5 parts of acetylated oxidized starch, 0.5 parts of animal bifidobacterium, 0.5 parts of streptococcus thermophilus, 1 part of resistant dextrin, 1 part of microcrystalline cellulose, and 1 part of magnesium stearate.

[0057] The preparation method thereof comprises the following steps:

[0058] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0059] Example 4

[0060] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0061] 10 parts of velvet antler polysaccharide, 8 parts of elm ear polysaccharide, 5 parts of echinacea, 7 parts of chia seeds, 7 parts of spirulina, 4 parts of gourd shell, 4 parts of black cumin seed oil, 1 part of melatonin, 3 parts of pea sprouts, 1 part of magnesium L-threonate, 3 parts of black garlic polysaccharide, 3 parts of peanut meal polysaccharide, 5 parts of pumpkin seeds, 1 part of acetylated oxidized starch, 1 part of animal Bifidobacterium, 1 part of Streptococcus thermophilus, 1.5 parts of resistant dextrin, 2 parts of microcrystalline cellulose, and 1.5 parts of magnesium stearate.

[0062] The preparation method thereof comprises the following steps:

[0063] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0064] Example 5

[0065] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0066] 15 parts of velvet antler polysaccharide, 10 parts of elm ear polysaccharide, 7 parts of echinacea, 8 parts of chia seeds, 10 parts of spirulina, 5 parts of gourd shell, 6 parts of black cumin seed oil, 3 parts of melatonin, 5 parts of pea sprouts, 3 parts of magnesium L-threonate, 6 parts of black garlic polysaccharide, 6 parts of peanut meal polysaccharide, 8 parts of pumpkin seeds, 2 parts of acetylated oxidized starch, 5 parts of animal bifidobacterium, 3 parts of streptococcus thermophilus, 2 parts of resistant dextrin, 3 parts of microcrystalline cellulose, and 3 parts of magnesium stearate.

[0067] The preparation method thereof comprises the following steps:

[0068] The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

[0069] Example 6

[0070] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0071] The difference from Example 4 is that it further includes 1 portion of oligofructose-nanoselenium.

[0072] Oligofructose-nanoselenium was prepared by dissolving 10g of oligofructose in 40mL of ethanol-water solution at room temperature. After the oligofructose was fully dissolved, 3.5g of sodium selenite was added. The solution was then incubated in a 50°C water bath at 2000 rpm in the dark. After the reaction, the reaction mixture was centrifuged at 10,000 rpm. The precipitate was washed three times with water and then centrifuged again at 10,000 rpm. The precipitate was dried and ground to obtain oligofructose-nanoselenium. The volume ratio of ethanol to water in the ethanol-water solution was 1:4. The oligofructose was selected from tetrose.

[0073] Example 7

[0074] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0075] The difference from Example 4 is that 3 portions of oligofructose-nanoselenium are also included.

[0076] Oligofructose-nanoselenium was prepared by dissolving 10g of oligofructose in 60mL of ethanol-water solution at room temperature. After the oligofructose was fully dissolved, 4g of sodium selenite was added. The solution was then incubated in a 60°C water bath at 2000 rpm in the dark. After the reaction, the reaction solution was centrifuged at 10,000 rpm. The precipitate was washed three times with water and then centrifuged again at 10,000 rpm. The precipitate was dried and ground to obtain oligofructose-nanoselenium. The volume ratio of ethanol to water in the ethanol-water solution was 1:4.

[0077] Example 8

[0078] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0079] The difference from Example 4 is that 5 portions of oligofructose-nanoselenium are also included.

[0080] Oligofructose-nanoselenium was prepared by dissolving 50g of oligofructose in 400mL of ethanol-water solution at room temperature. After the oligofructose was fully dissolved, 10g of sodium selenite was added. The solution was then incubated in a 65°C water bath at 2000 rpm in the dark. After the reaction, the reaction mixture was centrifuged at 10,000 rpm. The precipitate was washed three times with water and then centrifuged again at 10,000 rpm. The precipitate was dried and ground to obtain oligofructose-nanoselenium. The volume ratio of ethanol to water in the ethanol-water solution was 1:4.

[0081] Example 9

[0082] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0083] The difference from Example 4 is that it also includes 1 portion of zinc-coordinated DHA-nanoselenium.

[0084] Zinc-coordinated DHA-nanoselenium is prepared by the following steps:

[0085] (1) At room temperature, 10 g of galacto-oligosaccharide was dissolved in 40 mL of ethanol-water solution. After the galacto-oligosaccharide was fully dissolved, 4 g of sodium selenite was added. Then, the solution was reacted in a water bath at 55°C at a speed of 2000 r / min under light-proof conditions. After the reaction was completed, the reaction solution was set aside. The volume ratio of ethanol to water in the ethanol-water solution was 1:4.

[0086] (2) Add 5 g of DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add 5 g of zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

[0087] Example 10

[0088] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0089] The difference from Example 4 is that 3 portions of zinc-coordinated DHA-nanoselenium are also included.

[0090] Zinc-coordinated DHA-nanoselenium is prepared by the following steps:

[0091] (1) At room temperature, 10 g of galacto-oligosaccharide was dissolved in 60 mL of ethanol-water solution. After the galacto-oligosaccharide was fully dissolved, 4 g of sodium selenite was added. Then, the solution was reacted in a water bath at 55°C at a speed of 2000 r / min under light-proof conditions. After the reaction was completed, the reaction solution was set aside. The volume ratio of ethanol to water in the ethanol-water solution was 1:4.

[0092] (2) Add 5 g of DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add 4.5 g of zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

[0093] Example 11

[0094] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0095] The difference from Example 7 is that 5 parts of zinc-coordinated DHA-nanoselenium are also included.

[0096] Zinc-coordinated DHA-nanoselenium is prepared by the following steps:

[0097] (1) At room temperature, 50 g of galacto-oligosaccharide was dissolved in 400 mL of ethanol-water solution. After the galacto-oligosaccharide was fully dissolved, 15 g of sodium selenite was added. Then, the solution was reacted in a water bath at 65 ° C at a speed of 2000 r / min under light-proof conditions. After the reaction was completed, the reaction solution was set aside. The volume ratio of ethanol to water in the ethanol-water solution was 1:4.

[0098] (2) Add 25 g of DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add 25 g of zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

[0099] Example 12

[0100] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0101] The difference from Example 7 is that it further comprises 1 part of oligofructose-nanoselenium and 3 parts of zinc-coordinated DHA-nanoselenium.

[0102] Example 13

[0103] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0104] The difference from Example 7 is that it further comprises 3 parts of oligofructose-nanoselenium and 3 parts of zinc-coordinated DHA-nanoselenium.

[0105] Example 14

[0106] A nutritional health product for regulating immune function, comprising the following components in parts by weight:

[0107] The difference from Example 7 is that it further comprises 5 parts of oligofructose-nanoselenium and 3 parts of zinc-coordinated DHA-nanoselenium.

[0108] 1. Immunity test experiment:

[0109] (1) Test samples: compositions of Examples 1-14.

[0110] (2) Experimental animals: SPF grade Balb / c mice, weighing 20±2 g.

[0111] (3) Experimental environment: temperature is 22-25°C and relative humidity is 55-70%.

[0112] (4) Experimental method: Mice were randomly divided into 10 groups, 5 mice in each group, including experimental groups (a total of 14 groups, gavage of the composition of Examples 1-14 at 0.5 g / kg.bw) and control groups (gavage of equal weight of distilled water), and the mice were gavaged for 30 days.

[0113] (5) 1ConA-induced mouse spleen lymphocyte transformation experiment: After continuous gavage of the above-mentioned doses for 30 days, the mice were killed by cervical dislocation, and the spleens were removed aseptically. Single cell suspensions were prepared (cell concentration was 1×10 7 Mouse spleen cell suspensions were added to 24-well culture plates. For each treatment, duplicate wells (1 mL per well) were used. 50 μL of ConA was added to one well, while 50 μL of double-distilled water was added to the other well as a control. The plates were then incubated in a 5% CO2, 37°C CO2 incubator for 48 hours. Four hours before the end of the incubation period, 50 μL of MTT (5 mg / mL) was added per well and incubation continued. After the incubation period, 1 mL of DMSO was added to each well and mixed by pipetting to completely dissolve the purple crystals. The cells were then aliquoted into 96-well culture plates, with triplicate wells per well. The OD values were measured at 570 nm using a microplate reader. The optical density of the wells without ConA was subtracted from the optical density of the wells with ConA added to represent the lymphocyte proliferation capacity. Lymphocyte proliferation capacity = OD value of the ConA well - OD value of the control well. The results are shown in Table 1.

[0114] Table 1 Statistics of lymphocyte proliferation ability

[0115]

[0116]

[0117] Note: Different letters in a and b indicate significant differences between the control group and the experimental group.

[0118] As can be seen from Table 1, the health care product prepared in the embodiment of the present invention helps to enhance the proliferation ability of lymphocytes in mice and improve immunity.

[0119] 2. Mouse delayed hypersensitivity test experiment:

[0120] (1) Test samples: compositions of Examples 1-14.

[0121] (2) Experimental animals: SPF grade Balb / c mice, weighing 20±2 g.

[0122] (3) Experimental environment: temperature is 22-25°C and relative humidity is 55-70%.

[0123] (4) Experimental method: Mice were randomly divided into 10 groups, 5 mice in each group, including experimental groups (a total of 14 groups, gavaged with the composition of Examples 1-14 at 0.5 g / kg.bw) and control groups (gavaged with distilled water of equal weight). After continuous gavage once a day, on the 26th day, each mouse was intraperitoneally injected with 0.2 mL of 2% SRBC for immunization. After gavage for 4 days, the thickness of the left hind toe was measured, and the average value was obtained by measuring three times. Then, 20% SRBC was subcutaneously injected at the measurement site, 20 μL per mouse. The thickness of the left hind toe was measured 24 hours after injection, and the average value was obtained by measuring three times. The results are shown in Table 2.

[0124]

[0125]

[0126] As can be seen from Table 2, the health care products prepared according to the embodiments of the present invention can enhance the immune response of mice and improve their immunity.

[0127] 3. Test experiment of taking effect:

[0128] Test method: 120 men and 120 women aged 30-55 with weak constitution (all with varying degrees of symptoms such as loss of appetite, pale complexion, weak legs and feet, insomnia, dreaminess, palpitations, shortness of breath, and sweating) were selected. The men and women were randomly divided into 6 groups, with 20 people in each group. The men and women took the products prepared in Examples 1, 2, 4, 7, 10 and 13, respectively, once a day in the morning and evening, 1 g each time, for 8 consecutive weeks.

[0129] Changes in physical condition were recorded and analyzed. The evaluation criteria were as follows: (1) significantly effective, with easy falling asleep, significantly fewer nighttime awakenings, increased appetite, rosy complexion, strong legs and feet, and improved spirits; (2) effective, with faster falling asleep, fewer nighttime awakenings, increased appetite, gradual feeling of strength, and improved complexion; and (3) ineffective, with no significant changes. Records were kept every two weeks. The results are shown in Table 3.

[0130] Table 3 Statistics of the effects of taking

[0131]

[0132]

[0133]

[0134] As can be seen from Table 3, the health care products prepared by the present invention contain a large amount of nutrients such as bioactive substances and trace elements, which can meet the energy requirements of the body, promote protein synthesis, and can quickly and significantly improve the physical condition of people with weak constitutions. Moreover, the health care effects are not limited to a single gender and have a considerable effect on both men and women.

[0135] The present invention regulates immunity through the synergistic effect between the raw materials: (1) immune regulation and anti-inflammatory synergy, antler antler polysaccharide and elm ear polysaccharide synergize, Echinacea chinensis and black seed oil synergize, and reduce inflammatory factors through dual synergistic effect; (2) antioxidant network construction, melatonin, black garlic polysaccharide and gourd shell synergize, spirulina and chia seeds synergize, and further eliminate free radicals through dual synergistic effect; (3) intestinal health and flora regulation, animal bifidobacterium, thermophilic streptococcus and oligofructose-nanoselenium synergize, pea sprouts and pumpkin seeds synergize, and achieve intestinal health through flora regulation; (4) metabolic regulation, chia seeds and peanut meal polysaccharide synergize, L-magnesium threonate and melatonin synergize, regulate metabolism and anti-aging. The present invention further enhances immunity through the synergistic effect between the active ingredients without side effects.

[0136] In the present invention, the terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" mean that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0137] The foregoing description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are readily apparent to those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A nutritional health product for regulating immune function, characterized in that: Contains the following components in parts by weight: 5-15 parts of velvet antler polysaccharide and / or 5-15 parts of elm ear polysaccharide, 1-10 parts of Echinacea chinensis, 5-15 parts of chia seeds, 5-15 parts of spirulina, 1-10 parts of cucurbit shell, 1-10 parts of black cumin seed oil, 0.1-5 parts of melatonin, 1-10 parts of pea sprouts, 0.1-5 parts of magnesium L-threonate, 1-10 parts of black garlic polysaccharide, 1-10 parts of peanut meal polysaccharide, 0.1-8 parts of pumpkin seeds, 0.1-3 parts of acetylated oxidized starch, 0.1-5 parts of animal bifidobacterium, and 0.1-5 parts of thermophilic streptococcus.

2. The nutritional health product for regulating immune function according to claim 1, characterized in that: It also includes 0.1 to 5 parts of oligofructose-nanoselenium.

3. The nutritional health product for regulating immune function according to claim 2, characterized in that: The oligofructose-nanoselenium is prepared by the following steps: At room temperature, oligofructose is dissolved in an ethanol aqueous solution. After the oligofructose is fully dissolved, sodium selenite is added. Then, the solution is reacted in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, the reaction liquid is centrifuged at a speed of 10000 r / min. The precipitate is washed with water three times and then centrifuged at a speed of 10000 r / min. The precipitate is dried and ground to obtain oligofructose-nanoselenium.

4. The nutritional health product for regulating immune function according to claim 3, characterized in that: The ratio of the oligofructose to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, and the mass ratio of the oligofructose to sodium selenite is 2-5:

1.

5. The nutritional health product for regulating immune function according to claim 1 or 2, characterized in that: It also includes 0.1 to 5 parts of zinc-coordinated DHA-nanoselenium.

6. The nutritional health product for regulating immune function according to claim 5, characterized in that: The zinc-coordinated DHA-nanoselenium is prepared by the following steps: (1) Dissolve galactoligosaccharide in an ethanol aqueous solution at room temperature. After the galactoligosaccharide is fully dissolved, add sodium selenite. Then, react the solution in a water bath at 50-65°C at a speed of 2000 r / min under light-proof conditions. After the reaction is completed, set the reaction solution aside. (2) Add DHA aqueous solution to the above reaction solution, stir at a speed of 2000 r / min, then add zinc nitrate aqueous solution, stir at a speed of 2000 r / min, centrifuge the solution, wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Wash the precipitate with water three times, and then centrifuge at a speed of 10000 r / min. Dry and grind the precipitate to obtain zinc-coordinated DHA-nanoselenium.

7. The nutritional health product for regulating immune function according to claim 5, characterized in that: In step (1), the ratio of the oligofructose to the ethanol aqueous solution is 1-5 g:8 mL, the volume ratio of ethanol to water in the ethanol aqueous solution is 1:4, and the mass ratio of the oligofructose to sodium selenite is 2-5:

1.

8. The nutritional health product for regulating immune function according to claim 5, characterized in that: In step (2), the mass ratio of the galacto-oligosaccharide to DHA and zinc nitrate is 2:1:0.9-1.

9. The nutritional health product for regulating immune function according to any one of claims 1 to 8, characterized in that: The invention also includes pharmaceutical excipients, which are one or more of resistant dextrin, microcrystalline cellulose, and magnesium stearate.

10. A method for preparing a nutritional health product for regulating immune function according to claim 9, characterized in that: The following steps are involved: The above raw materials are weighed separately, and Echinacea, chia seeds, spirulina, gourd shell, black cumin seed oil, pea sprouts and pumpkin seeds are crushed into ultrafine powder with a particle size of more than 200 meshes. The remaining raw materials are added to the ultrafine powder, mixed evenly, and then granulated, dried and sterilized to obtain the product.

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