Full-automatic chromosome sample harvesting system and method

Abstract

The invention discloses a full-automatic chromosome sample harvesting system and a full-automatic chromosome sample harvesting method, which are characterized in that an instrument box body, a centrifugal servo control device, a sample imbedding detection device, a cleaning and supernate discarding device, a waste liquid treatment device, a reagent adding device, a reagent storage device, a sample vibration device, a thermotank inner bin device and a control device are integrated in the system; a PLC (Programmable Logic Controller) is adopted to automatically control the system, and the whole process of detecting a put sample, centrifuging, discarding supernate, cleaning, uniformly shaking sediment, performing hypotonic treatment and fixing is automatically completed; the device has the advantages of being high in automation degree, low in cost, high in module integration degree, large in sample capacity, high in harvesting efficiency, good in chromosome harvesting sample quality, high in consistency and the like, the requirement for chromosome sample harvesting in the medical diagnosis and experimental research process can be met, and the efficiency of chromosome sample harvesting is improved while the quality and the consistency of the harvested samples are ensured. And the possibility of infection to operators can also be reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices and chromosome preparation, and in particular to a system and method for fully automatic chromosome sample harvesting. Background Art

[0002] In cytogenetic research and clinical diagnosis, chromosome analysis is a key technology for identifying chromosomal abnormalities (such as numerical abnormalities and structural aberrations). Chromosome harvesting, as its core step, aims to efficiently and accurately isolate metaphase chromosome division phases or micronuclei from cell culture samples, providing high-quality samples for subsequent experiments such as karyotyping and fluorescence in situ hybridization (FISH). However, traditional chromosome harvesting methods mainly rely on manual operations, involving steps such as cell culture, hypotonic treatment, fixation, dripping, and staining. These steps not only require high technical experience and skills of the operator, but also lead to uneven sample quality due to human variability. At the same time, there are problems such as low efficiency, high error rate, poor sample consistency, and high risk of contamination. For example, inaccurate time and temperature control during hypotonic treatment can cause cell rupture or chromosome structural abnormalities. The amount and time deviation of the fixative in the fixation step can affect chromosome morphology. In addition, the reproducibility of manual operations is difficult to ensure, which seriously restricts the needs of large-scale sample processing and standardized experiments.

[0003] To address these issues, the development of a fully automated chromosome harvester is crucial. Replacing manual operations with automated technology significantly improves experimental efficiency and consistency, reduces human error and contamination risks, and enables precise control of key steps like hypotonicity and fixation, ensuring high-quality and reproducible chromosome samples and meeting the demands for standardized, high-throughput chromosome analysis in clinical diagnosis and research. Summary of the Invention

[0004] The present invention addresses the deficiencies of the prior art and provides a fully automated chromosome sample harvesting system and method. The system integrates a centrifugal servo control device, a sample placement detection device, a cleaning and supernatant discarding device, a waste liquid treatment device, a reagent addition device, a reagent storage device, a sample vibration device, a constant temperature chamber device, and a controller. The system is automatically controlled by a PLC programmable controller, automatically completing the entire process of sample placement detection, centrifugation, supernatant discarding, cleaning, sedimentation shaking, hypotonic treatment, and fixation. The system has the advantages of high automation, low cost, high module integration, large sample capacity, high harvesting efficiency, and good quality and consistency of harvested chromosome samples. It can meet the needs of chromosome sample harvesting in medical diagnosis and experimental research, while ensuring the quality and consistency of harvested samples and reducing the possibility of infection to operators. The system is used to automatically harvest metaphase chromosome division phases or micronuclei from cell suspensions and is widely used in cytogenetics laboratories, clinical diagnosis, genetic disease research, gene therapy, and biopharmaceuticals.

[0005] The specific technical solution for achieving the purpose of the present invention is:

[0006] A fully automatic chromosome sample harvesting system includes an instrument housing, a centrifugal servo control device, a sample placement detection device, a cleaning and supernatant discarding device, a waste liquid treatment device, a reagent adding device, a reagent storage device, a sample vibration device, a constant temperature chamber device, and a control device;

[0007] The instrument box is a hexahedron, with a top plate and a bottom plate. Inside the box, there are a first-layer partition, a second-layer partition and a third-layer partition in order from top to bottom. A touch screen is provided on the surface, an operating cabin cover is provided between the top plate and the first-layer partition, and a reagent storage door is provided between the third-layer partition and the bottom plate. An ultraviolet lamp, a reagent adding device seat, a lighting lamp and a filter are provided on the bottom surface of the top plate, and a heat dissipation device is provided on the outside of the top surface that passes through the filter.

[0008] A turntable hole is provided on the first layer of the partition plate, and a cleaning and supernatant discarding device seat, a stationary liquid-hypotonic liquid waste tank seat and a supernatant discarding waste tank seat are provided around the turntable hole;

[0009] A centrifugal disc shaft seat is provided in the center of the second-layer partition, a constant temperature box inner compartment heating cavity seat is provided around it, and a centrifugal servo motor seat is provided on one side;

[0010] The bottom surface of the second-layer partition is provided with a sample placement detection device seat, a sample vibration device seat and a sample tightening device seat;

[0011] The centrifugal servo control device consists of a centrifugal servo motor, a centrifugal disk, a centrifugal shaft, a centrifugal bearing seat and a centrifugal transmission structure; the centrifugal servo motor is arranged on the centrifugal servo motor seat on the surface of the second-layer partition; the centrifugal transmission structure is arranged on the bottom surface of the second-layer partition, and the centrifugal bearing seat is arranged on the centrifugal disk shaft seat on the surface of the second-layer partition; the centrifugal disk is located in the turntable hole on the first-layer partition.

[0012] The sample placement detection device is composed of a diffuse reflection sensor group, a detection vertical movement module and a detection tightening rubber block;

[0013] The diffuse reflection sensor group and the detection and tightening rubber block are sequentially arranged at the output end of the detection vertical movement module;

[0014] The detection vertical movement module is provided with a detection vertical movement motor;

[0015] The sample placement detection device is provided with a vertical moving module on the sample placement detection device seat on the bottom surface of the second-layer partition board after detection, and the detection tightening rubber block corresponds to the sample position hole on the centrifugal disk;

[0016] The reagent adding device is composed of reagent bottles of several reagents, reagent bottle bases and liquid level sensors;

[0017] A liquid level sensor is provided on the reagent bottle, and the reagent bottle is arranged on the bottom plate inside the reagent storage cabin door via a reagent bottle base.

[0018] The cleaning and supernatant liquid discarding device is composed of a cleaning and waste liquid suction component, a cleaning and tightening component and a clean water gear pump;

[0019] The cleaning and waste liquid suction component is composed of a supernatant liquid suction needle group, a cleaning two-dimensional moving module, a waste liquid suction electromagnetic valve group and a supernatant liquid discarding waste liquid tank;

[0020] The cleaning and waste liquid suction component of the cleaning and discarding supernatant device is arranged on the cleaning and discarding supernatant device seat of a layer of partition through a cleaning two-dimensional mobile module;

[0021] The supernatant liquid waste tank is arranged on a supernatant liquid waste tank seat of a layer of partition;

[0022] The clean water gear pump is arranged on the bottom surface of the three-layer partition board.

[0023] The sample vibration device consists of a vibration vertical movement module, a vibration servo platform, a vibration rubber block, an eccentric disk, a vibration servo motor and a vibration shaft; the sample vibration device is arranged on the sample vibration device seat on the bottom surface of the second-layer partition through the vibration vertical movement module, and the vibration rubber block corresponds to the sample station hole on the centrifugal disk.

[0024] The constant temperature box inner chamber device is composed of a constant temperature box inner chamber heating cavity, a working chamber upper cover, a sample placement cover and a door opening and closing sensor;

[0025] The working chamber upper cover is arranged on the top of the heating cavity of the constant temperature box, the sample placement cover is arranged on the working chamber upper cover, and the door opening and closing sensor is arranged just below the sample placement cover, between the sample placement cover and the sample centrifuge rotor assembly;

[0026] The constant temperature box inner chamber device is arranged on the constant temperature box inner chamber heating cavity seat of the second layer of the partition through the constant temperature box inner chamber heating cavity.

[0027] The reagent adding device is composed of a top liquid injection component, a hypotonic solution adding component, a fixative solution adding component and a fixative solution-hypotonic solution waste liquid tank;

[0028] The top liquid injection assembly is arranged on the reagent adding device seat of the top plate through the reagent vertical moving module;

[0029] The hypotonic liquid adding component is arranged on the bottom plate inside the reagent storage compartment door;

[0030] The stationary liquid-hypotonic liquid waste tank is arranged on the stationary liquid-hypotonic liquid waste tank seat of a layer of partition;

[0031] The fixing liquid adding component is arranged on the bottom plate inside the reagent storage cabin door.

[0032] The waste liquid treatment device is composed of a waste liquid diaphragm pump, a waste liquid barrel, a waste liquid barrel filling solenoid valve, a waste liquid barrel-cleaning solenoid valve and a waste liquid barrel-fixed liquid / hypotonic liquid solenoid valve:

[0033] The waste liquid treatment device is arranged on the bottom plate inside the reagent storage cabin door.

[0034] The control device is a PLC programmable controller, which is electrically connected to the touch screen display, centrifugal servo control device, sample placement detection device, cleaning and supernatant discarding device, waste liquid treatment device, reagent addition device, reagent storage device, sample vibration device and electrical parts of the constant temperature chamber device on the instrument box;

[0035] The control device is arranged on the three-layer partition board.

[0036] Furthermore, the centrifugal disk of the centrifugal servo control device is coaxially connected to the centrifugal bearing seat via the centrifugal shaft;

[0037] The centrifugal servo motor is connected to the centrifugal shaft via a centrifugal transmission structure;

[0038] A plurality of sample station holes are evenly distributed along the circumference of the centrifugal disk, and a sample centrifugal rotor assembly is arranged in the sample station holes.

[0039] Furthermore, the sample centrifuge rotor assembly is composed of a sample tube rack mounting seat, a centrifugal system limit block, a sample tube rack cover, a sample tube rack bracket, a sample tube rack, a vibrating pendulum rod, a test tube and a vibrating pendulum rubber block;

[0040] The sample tube rack and the sample tube rack cover are sequentially arranged at the top end of the vibrating pendulum rod, the vibrating pendulum rubber block is arranged at the tail end of the vibrating pendulum rod, and the test tube is placed in the sample tube rack;

[0041] The sample tube rack support of the sample centrifugal rotor assembly is placed in the sample station hole of the centrifugal disk and is connected to the centrifugal disk via the sample tube rack mounting seat.

[0042] Furthermore, the supernatant aspirating needle assembly of the cleaning and supernatant discarding device is arranged at the output end of the cleaning two-dimensional moving module;

[0043] The supernatant aspirating needle tube group is provided with a plurality of independently controlled supernatant aspirating needles;

[0044] The waste liquid suction solenoid valve group is provided with several waste liquid suction solenoid valves;

[0045] The supernatant sample aspirating needle is connected to the waste liquid aspirating electromagnetic valve;

[0046] The cleaning two-dimensional moving module is provided with a cleaning horizontal moving motor and a cleaning vertical moving motor;

[0047] The cleaning and tightening assembly is composed of a cleaning and tightening vertical moving module and a cleaning and tightening rubber block;

[0048] The cleaning and tightening rubber block is arranged at the output end of the cleaning and tightening vertical moving module;

[0049] The cleaning, tightening and vertical moving module is provided with a cleaning, tightening and vertical moving motor;

[0050] The cleaning and tightening assembly is arranged on the sample tightening device seat on the bottom surface of the second-layer partition through the cleaning and tightening vertical moving module, and the cleaning and tightening rubber block corresponds to the sample position hole on the centrifugal disk;

[0051] The clean water gear pump is connected between the supernatant waste tank and the reagent bottle.

[0052] Furthermore, the vibration servo platform of the sample vibration device is provided at the output end of the vibration vertical movement module;

[0053] The vibration shaft, eccentric disk and vibration rubber block are sequentially arranged at the output end of the vibration servo motor;

[0054] The vibration vertical movement module is provided with a vibration vertical movement motor;

[0055] The vibration servo motor is arranged on the vibration servo platform.

[0056] Furthermore, the top liquid injection assembly of the reagent adding device is composed of a reagent vertical moving module, a reagent rotating motor, a fixative sample injection needle group, a hypotonic solution sample injection needle group, and a reagent injection system suspension component;

[0057] The fixed solution sample injection needle tube group and the hypotonic solution sample injection needle tube group are respectively arranged on both sides of the reagent injection system suspension component;

[0058] The fixative solution injection needle tube group is composed of several independently controlled fixative solution injection needles;

[0059] The hypotonic solution sampling needle tube set is composed of several independently controlled hypotonic solution sampling needles;

[0060] The reagent rotating motor is provided at the output end of the reagent vertical moving module, and the reagent injection system suspension is provided at the output end of the reagent rotating motor;

[0061] The reagent vertical movement module is provided with a reagent vertical movement motor;

[0062] The hypotonic solution adding assembly is composed of a hypotonic solution solenoid valve assembly, a hypotonic solution infusion tube assembly, a hypotonic solution heating bottle, a hypotonic solution inlet solenoid valve, a hypotonic solution inlet / outlet solenoid valve, and a hypotonic solution gear pump;

[0063] The hypotonic liquid adding component is divided into a hypotonic liquid bottle inlet circuit and a hypotonic liquid bottle outlet circuit;

[0064] First, the hypotonic solution bottle inlet circuit is connected from the reagent bottle of the reagent adding device through the hypotonic solution-inlet / outlet electromagnetic valve, the hypotonic solution gear pump, the hypotonic solution-inlet electromagnetic valve to the hypotonic solution heating bottle pipeline;

[0065] Secondly, the hypotonic liquid bottle outlet circuit is connected by the hypotonic liquid heating bottle through the hypotonic liquid-inlet / outlet electromagnetic valve, the hypotonic liquid gear pump, the hypotonic liquid electromagnetic valve group, the hypotonic liquid infusion tube group to the hypotonic liquid adding needle group of the top injection assembly;

[0066] The hypotonic liquid heating bottle is provided with a hypotonic liquid bottle heater and a hypotonic liquid heating bottle insulation layer;

[0067] The fixing solution adding assembly is composed of a fixing solution solenoid valve group, a fixing solution infusion tube group, a mixer motor moving module, a mixed solution syringe, a mixer-methanol solenoid valve, a mixer-acetic acid solenoid valve and a mixer flow channel;

[0068] The fixing liquid inlet circuit of the fixing liquid adding component is divided into two paths;

[0069] First, adding methanol: connect the methanol reagent bottle of the reagent adding device through the mixer-methanol solenoid valve, the mixer flow channel to the mixed liquid syringe pipeline;

[0070] Second, add acetic acid: connect the acetic acid reagent bottle of the reagent adding device through the mixer-acetic acid solenoid valve, the mixer flow channel to the mixed liquid syringe pipeline;

[0071] The methanol and acetic acid reagents are mixed in a mixed liquid syringe;

[0072] The fixative liquid outlet circuit of the fixative adding component is connected by a mixed liquid syringe through a mixer flow channel, a fixative liquid electromagnetic valve group, a fixative liquid infusion tube group to a fixative liquid adding needle tube group pipeline of the top injection component.

[0073] Furthermore, the waste liquid recovery circuit of the waste liquid treatment device is divided into two circuits:

[0074] First, the supernatant waste liquid tank on the cleaning and waste liquid suction assembly is connected through the waste liquid barrel-cleaning solenoid valve, the waste liquid diaphragm pump, the waste liquid barrel filling solenoid valve to the waste liquid barrel pipeline;

[0075] Secondly, the reagent is added from the fixative-hypotonic solution waste liquid tank of the device through the waste liquid barrel-fixative solution / hypotonic solution electromagnetic valve, the waste liquid diaphragm pump, the waste liquid barrel filling electromagnetic valve to the waste liquid barrel pipeline.

[0076] A method for fully automatic chromosome sample harvesting is provided. The method implements fully automatic chromosome sample harvesting based on the system, and specifically comprises the following steps:

[0077] a. Steps for taking and placing consumables: Before the system starts working, clear the waste liquid left in the waste liquid bucket and ensure that the corresponding reagent capacity in the reagent bottle is sufficient;

[0078] b. Device reset step: After completing step a, start the system and operate the control device to reset the device;

[0079] c. Reagent detection step: After executing step b, read the liquid level sensors of several reagents to confirm that the reagent capacity is sufficient;

[0080] d. Remaining test tube detection step: After executing step c, the test tube that may be left in the sample centrifuge rotor assembly is detected; the diffuse reflection sensor group of the sample is placed in the detection device to determine whether there is a test tube left on the sample centrifuge rotor assembly;

[0081] If it is detected that there are test tubes left on the sample centrifuge rotor assembly, the touch screen will display the location of the remaining test tube and prompt the user to remove the test tube. After confirming that there are no test tubes, the diffuse reflection sensor group will detect the next set of sample centrifuge rotor assemblies.

[0082] Repeat the detection steps until all sample centrifuge rotor assemblies are tested;

[0083] e. Test tube placement step: After completing step d, begin test tube placement; enter the number of test tubes to be placed on the touch screen, and then, according to the prompts, place the test tubes with weights or samples in the sample tube rack position specified by the sample centrifuge rotor assembly;

[0084] f. Centrifugation step: After completing step e, the test tube containing the weight or sample is subjected to high-speed centrifugation; the control device drives the centrifugal servo motor to rotate at a speed of 1000-1400 RPM for 5-10 minutes, thereby separating the cells and other substances in the test tube containing the sample;

[0085] g. Step of discarding the supernatant: After performing step f, the supernatant is removed from the test tube after centrifugation by absorbing the supernatant from the counterweight or sample; the cleaning top tight vertical moving motor driving the cleaning top tight vertical moving module makes the cleaning top tight rubber block and the sample centrifuge rotor assembly vibrating pendulum rubber block against;

[0086] Drive the cleaning horizontal moving motor on the cleaning two-dimensional moving module to move the supernatant aspirating needle tube group to the supernatant discarding position;

[0087] Driving the cleaning vertical moving motor on the cleaning two-dimensional moving module to move the supernatant aspirating needle of the supernatant aspirating needle tube assembly downward into the test tube with the counterweight or sample placed on the sample centrifugal rotor assembly;

[0088] Open the waste liquid diaphragm pump and the corresponding waste liquid suction solenoid valve, then slowly drive the cleaning vertical moving motor on the cleaning two-dimensional moving module to drive the supernatant aspirating needle to move upward to the end position of discarding the supernatant;

[0089] After the aspiration is completed, the cleaning vertical moving motor is reset, and the waste liquid diaphragm pump and the corresponding waste liquid aspiration solenoid valve are closed, and then the cleaning and tightening vertical moving motor is reset, thereby completing the supernatant discarding process of all test tubes with counterweights or samples placed on the single sample centrifuge rotor assembly;

[0090] Then, the centrifugal servo motor is controlled to move all the test tubes with weights or samples placed on the next group of sample centrifuge rotor assemblies to the supernatant discarding position to complete the supernatant discarding process; until the supernatant discarding operation is completed for all the test tubes with weights or samples placed on the sample centrifuge rotor assemblies;

[0091] h. Needle cleaning step: After completing step g, clean the supernatant aspiration needle assembly to prevent the supernatant waste liquid absorbed in step g from remaining on the supernatant aspiration needle; move the supernatant aspiration needle assembly to the top of the supernatant waste liquid tank;

[0092] Control the supernatant aspirating needle to be inserted into the supernatant waste tank;

[0093] Then, the control device drives the clean water gear pump to start working and inject clean water into the supernatant waste tank;

[0094] After the clean water in the waste liquid tank for discarding the supernatant reaches the set height, turn off the clean water gear pump; then, open the waste liquid diaphragm pump and all the waste liquid suction solenoid valves. The waste liquid diaphragm pump draws water, causing it to flow through the supernatant aspiration needle and the corresponding waste liquid suction solenoid valve, and then through the waste liquid diaphragm pump and the waste liquid bucket filling solenoid valve, and finally flows into the waste liquid bucket, thereby completing a single cleaning of the supernatant aspiration needle tube group;

[0095] After completing a single cleaning, pour clean water into the waste liquid tank to discard the supernatant;

[0096] After repeating the above steps several times, the cleaning vertical moving motor on the cleaning two-dimensional moving module is reset to complete the cleaning process of the supernatant aspirating needle tube group;

[0097] i. Sedimentation and Shaking Step: After completing step h, the sample centrifuge rotor assembly is subjected to sedimentation and shaking with the counterweight or sample test tube; the vibration vertical movement motor on the vibration vertical movement module is driven to cause the vibration rubber block to contact the vibration pendulum rubber block of the sample centrifuge rotor assembly; at this time, the vibration servo motor is driven to move, and the vibration servo motor and the sample centrifuge rotor assembly are vibrated together with the counterweight or sample test tube;

[0098] After the vibration ends, reset the vibration vertical movement module;

[0099] Subsequently, the next set of sample centrifuge rotor assemblies on the centrifuge disk is brought to the sample shaking position, and the sedimentation and shaking process is repeated until the sedimentation and shaking operation of the test tubes with weights or samples placed on all sample centrifuge rotor assemblies is completed;

[0100] j. Hypotonic Treatment Step: After completing step i, the test tube containing the weight or sample is treated with hypotonic solution to swell the sample cells, disperse the chromosome sample, and complete the stretching and separation process. Before adding the hypotonic solution, ensure that the temperature of the heating chamber in the incubator has reached 37°C; only after reaching this temperature can subsequent operations be initiated.

[0101] For the hypotonic treatment operation of placing a counterweight or a sample tube on each sample centrifuge rotor assembly, the hypotonic solution in the hypotonic solution heating bottle is replenished to the highest point before the start of each round;

[0102] Driving the vibration vertical movement motor on the vibration vertical movement module to make the vibration rubber block and the vibration pendulum rubber block of the sample centrifugal rotor assembly press against each other;

[0103] Then, the reagent rotating motor is controlled to move the hypotonic solution sample injection needle assembly to the waste liquid discharge position, and the hypotonic solution sample injection needle is inserted into the fixative-hypotonic solution waste liquid tank;

[0104] Then open the hypotonic liquid gear pump, hypotonic liquid-inlet / outlet solenoid valve and all hypotonic liquid solenoid valves to discharge the cooled hypotonic liquid at the front end of the hypotonic liquid sampling needle; at the same time, open the waste liquid bucket-fixative liquid / hypotonic liquid solenoid valve and the waste liquid diaphragm pump to pump the discharged cooled hypotonic liquid into the waste liquid bucket through the waste liquid bucket filling solenoid valve;

[0105] After all the cooled hypotonic liquid at the front end of the hypotonic liquid sampling needle is discharged, the reagent vertical moving motor on the reagent vertical moving module is reset;

[0106] Then, the hypotonic solution adding needle assembly is driven to move to the hypotonic solution adding position, and the hypotonic solution adding needle is inserted into the corresponding test tube with the counterweight or sample placed therein;

[0107] At this time, the hypotonic fluid gear pump and the hypotonic fluid solenoid valve of the test tube containing the counterweight or sample are opened, and 8 ml of hypotonic fluid at a temperature of 37°C is injected; after confirming through sensor feedback that the volume of hypotonic fluid added to the test tube containing the counterweight or sample reaches 8 ml, the relevant solenoid valve is closed; and the reagent vertical movement motor on the vertical movement module is reset;

[0108] Then, the precipitation and shaking process in step i is performed to complete the hypotonic treatment operation of all the test tubes containing weights or samples on the single sample centrifuge rotor assembly;

[0109] Next, the next set of sample centrifuge rotor assemblies on the centrifuge disk is brought to the position for adding hypotonic solution, and the above steps are repeated to complete the hypotonic treatment operation of the test tubes with weights or samples placed on all sample centrifuge rotor assemblies;

[0110] k. Fixation step: After completing step j, all tubes containing weights or samples are fixed with a fixative solution to stabilize the structure of the chromosome sample for subsequent staining, dehydration, and clearing.

[0111] When using for the first time, check whether the fixative is fresh; if so, continue with the subsequent operation;

[0112] For each sample centrifuge rotor assembly, the total volume of fixative required for all test tubes with weights or samples is calculated before each round begins. This volume is then compared with the current volume of fixative available. If the volume is sufficient, the process continues. If the volume is insufficient, methanol and acetic acid are added to the tubes based on the difference.

[0113] Under the condition that the total volume of the fixative is sufficient, the sample centrifugal rotor assembly on the centrifugal disk is brought to the position for adding the fixative; then the vibration vertical movement motor on the vibration vertical movement module is driven to make the vibration rubber block and the vibration pendulum rubber block of the sample centrifugal rotor assembly press against each other;

[0114] Then, the fixative solution injection needle tube assembly is controlled to be located directly above the sample centrifuge rotor assembly, and the fixative solution injection needle is inserted into the corresponding test tube containing the counterweight or sample;

[0115] Subsequently, the solenoid valve of the fixative corresponding to the number of the test tube with the counterweight or sample is opened, and the mixer motor is operated according to the sample volume to complete the operation of adding the fixative to all the test tubes with the counterweight or sample on the single sample centrifuge rotor assembly;

[0116] Subsequently, the corresponding fixative solenoid valve is closed, and the reagent vertical movement motor on the reagent vertical movement module is reset; then, step i is performed to precipitate and shake evenly, completing the fixation operation of all test tubes containing counterweights or samples on the single sample centrifuge rotor assembly;

[0117] After the fixation operation is completed for all the test tubes with weights or samples placed on the centrifuge rotor assembly, perform step f, centrifuge, step g, discard the supernatant, and step h, rinse the needle tube. After completing steps f, g, and h, perform step i again, precipitate and shake well, thus completing the first fixation process.

[0118] After the first fixation process is completed, there are second and third fixation processes;

[0119] The second and third fixation processes are the same as the first fixation process;

[0120] 1. Test tube removal step: After completing step k, all test tubes containing weights or samples are removed after processing; the chromosome samples obtained from the test tubes containing samples can be used to prepare subsequent chromosome sample slides for observation and karyotype analysis under a microscope.

[0121] Bring the sample centrifuge rotor assembly on the centrifuge disk to the test tube placement position. The touch screen will then prompt the user to remove all test tubes with weights or samples placed on the current sample centrifuge rotor assembly. After removing all test tubes, press the confirmation button on the touch screen.

[0122] After the confirmation button is pressed, the control device reads the diffuse reflection sensor group to perform test tube detection. After confirming that the counterweight or sample test tube placed on the current sample centrifugal rotor assembly has been completely removed, the centrifugal servo motor is driven to move, and the next set of sample centrifugal rotor assemblies on the centrifugal disk are brought to the test tube insertion position. The above test tube removal process is repeated until the counterweight or sample test tube on all sample centrifugal rotor assemblies are removed, completing the test tube removal step.

[0123] At this point, the chromosome sample harvesting process is completed.

[0124] The beneficial effects of the present invention are:

[0125] The present invention realizes a fully automatic chromosome sample harvesting process, integrating all steps of chromosome sample harvesting into one. The automated process of the system replaces manual operation, thus avoiding the influence of human intervention on the chromosome sample harvesting process, effectively preventing operators from being infected, and avoiding the occurrence of nosocomial contamination.

[0126] The present invention can input the number of samples to be operated through the console interface, and flexibly adjust the amount of various reagents according to different sample volumes, which has high flexibility in use and can effectively save the amount of reagents;

[0127] The present invention can support the simultaneous operation of up to 96 chromosome samples, which can meet the needs of harvesting multiple samples simultaneously while ensuring sample quality, greatly improving the efficiency of sample harvesting and saving the time cost of sample harvesting;

[0128] The present invention wraps a layer of constant temperature protective material around the interior of the constant temperature box and the periphery of the hypotonic fluid heating bottle, and keeps the heater in working condition throughout the chromosome sample harvesting process, thereby ensuring that the temperature conditions of the entire process are met while greatly reducing energy consumption.

[0129] During the process of aspirating the supernatant after centrifugation, the present invention uses a needle with precise specifications as the supernatant aspirating needle, and cooperates with a stepping motor capable of achieving precise control. While ensuring that the upper layer of the supernatant can be completely aspirated, the impact on the sediment such as cells in the lower layer during the aspiration process can be controlled to a very small range;

[0130] During the cleaning process, the present invention uses a clean water gear pump to extract water flow, and with a needle of fine and strict specifications, it can stably control the flow rate of clean water, ensuring that the entire cleaning process is carried out smoothly. Through multiple cycles of cleaning and waste liquid extraction, it is ensured that the supernatant sampling needle is thoroughly cleaned and no residual liquid is left, effectively avoiding contamination of other samples when it is reused later;

[0131] During the addition of hypotonic solution, the present invention uses a sensor to provide feedback on the amount of hypotonic solution used. Combined with a hypotonic solution sample needle with precise specifications, it can ensure that the volume of hypotonic solution added to each test tube is strictly controlled at 8 ml, thereby reducing the differences in the harvested chromosome samples caused by different reagent addition amounts.

[0132] During the process of adding the fixative, the present invention uses a stepper motor in conjunction with a specially customized syringe tube to prepare the fixative immediately. By strictly controlling the moving speed and number of steps of the stepper motor, it is ensured that the two reagents, methanol and acetic acid, used to prepare the fixative are fully mixed, and that air is not mixed in during the preparation process to contaminate the reagents and affect the subsequent harvesting process.

[0133] The present invention can monitor the operation of each electrical appliance in real time. When an electrical appliance is found to be working abnormally, the relevant process can be terminated in time and feedback on the abnormal situation can be provided to ensure that the loss can be minimized.

[0134] In the present invention, there are several fans on the top and middle layers, which can be adjusted according to the needs of use to ensure good heat dissipation conditions of the device;

[0135] The present invention is equipped with an ultraviolet lamp, which can disinfect the inside of the system through the system's built-in disinfection program. It also supports users to manually control the ultraviolet lamp to disinfect the inside of the system according to usage needs;

[0136] The present invention is equipped with two filters inside. The filters have a four-layer filter structure consisting of a fine mesh primary filter layer, a HEPA filter layer, and two layers of activated carbon filter layers. They can fully filter the gas inside the device to achieve the purpose of purifying the air.

[0137] The electrical appliance part of the present invention includes a photoelectric induction switch and a precision sensor, which can effectively provide feedback on the operating conditions of each electrical appliance in the system, and make adjustments based on the feedback to achieve precise control. BRIEF DESCRIPTION OF THE DRAWINGS

[0138] Figure 1 It is a structural schematic diagram of the instrument box of the present invention;

[0139] Figure 2 This is a schematic diagram of the structure of the layered instrument case of the present invention;

[0140] Figure 3 This is a schematic structural diagram of a first-layer partition of the instrument case of the present invention;

[0141] Figure 4 This is a schematic structural diagram of the second-layer partition of the instrument box of the present invention;

[0142] Figure 5 Schematic diagram of the structure of the centrifugal servo control device of the present invention;

[0143] Figure 6 This is a bottom view of the structure of the detection device in which the sample is placed according to the present invention;

[0144] Figure 7 This is a schematic structural diagram of the cleaning and tightening assembly of the present invention;

[0145] Figure 8 It is a structural schematic diagram of the sample vibration device of the present invention;

[0146] Figure 9 This is a schematic structural diagram of the internal compartment device of the constant temperature box of the present invention;

[0147] Figure 10 This is a schematic structural diagram of the top liquid injection assembly on the reagent adding device of the present invention;

[0148] Figure 11 This is a schematic structural diagram of a hypotonic solution adding component on the reagent adding device of the present invention;

[0149] Figure 12 This is a schematic structural diagram of a fixing solution adding component on a reagent adding device of the present invention;

[0150] Figure 13 It is a structural schematic diagram of the waste liquid treatment device of the present invention;

[0151] Figure 14 It is a structural schematic diagram of the sample centrifuge rotor assembly of the present invention;

[0152] Figure 15 It is a schematic diagram of the partial structure of the bottom plate of the instrument box of the present invention;

[0153] Figure 16 It is a schematic diagram of the partial structure of the bottom surface of the top plate of the instrument box of the present invention. DETAILED DESCRIPTION

[0154] The present invention is described in detail below with reference to the accompanying drawings and embodiments.

[0155] See Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 16 The present invention provides a fully automatic chromosome sample harvesting system, comprising an instrument housing 1, a centrifugal servo control device 2, a sample placement detection device 3, a cleaning and supernatant discarding device 4, a waste liquid treatment device 5, a reagent adding device 6, a reagent storage device 7, a sample vibration device 8, a constant temperature chamber inner compartment device 9, and a control device 10.

[0156] The instrument case 1 is a hexahedron, and is provided with a top plate 11 and a bottom plate 15. A first-layer partition 12, a second-layer partition 13, and a third-layer partition 14 are arranged in order from top to bottom in the case. A touch screen 101 is provided on the surface, an operating cabin cover 19 is provided between the top plate 11 and the first-layer partition 12, and a reagent storage door 16 is provided between the third-layer partition 14 and the bottom plate 15; an ultraviolet lamp 111, a reagent adding device seat 112, an illuminating lamp 113, and a filter 117 are provided on the bottom surface of the top plate 11, and a heat dissipation device 118 is provided on the outside of the top surface that passes through the filter 117;

[0157] The first layer of the partition plate 12 is provided with a turntable hole 121, and the periphery of the turntable hole 121 is provided with a cleaning and supernatant discarding device seat 122, a fixed liquid-hypotonic liquid waste tank seat 124 and a supernatant discarding waste tank seat 125;

[0158] A centrifugal disc shaft seat 131 is provided in the center of the second-layer partition 13, a constant temperature box inner compartment heating cavity seat 133 is provided around it, and a centrifugal servo motor seat 132 is provided on one side;

[0159] A sample placement detection device seat 134 , a sample vibration device seat 135 , and a sample tightening device seat 136 are provided on the bottom surface of the second-layer partition 13 .

[0160] See Figure 1 、 Figure 2 、 Figure 16 Furthermore, the ultraviolet lamp 111, heat dissipation device 118 and filter 117 are used to disinfect, dissipate heat and filter the air of the instrument box 1; there are four filter layers inside the filter 117: a fine sand mesh primary filter layer, a HAPA filter layer and two activated carbon filter layers.

[0161] See Figure 2 、 Figure 4 、 Figure 5 The centrifugal servo control device 2 is composed of a centrifugal servo motor 20, a centrifugal disc 23, a centrifugal shaft 24, a centrifugal bearing seat 25 and a centrifugal transmission structure 26;

[0162] The centrifugal disc 23 is concentrically connected to the centrifugal bearing seat 25 via the centrifugal shaft 24;

[0163] The centrifugal servo motor 20 is mounted on a centrifugal servo motor seat 132 on the surface of the second-layer partition 13;

[0164] The centrifugal transmission structure 26 is provided on the bottom surface of the second-layer partition 13, and the centrifugal servo motor 20 is connected to the centrifugal shaft 24 via the centrifugal transmission structure 26;

[0165] The centrifugal disk 23 is provided with a plurality of sample station holes 231 evenly distributed along the circumference thereof, and a sample centrifugal rotor assembly 27 is provided in the sample station holes 231;

[0166] The centrifugal bearing seat 25 is disposed on the centrifugal disc shaft seat 131 on the surface of the second-layer partition plate 13 , and the centrifugal disc 23 is located in the turntable hole 121 on the first-layer partition plate 12 .

[0167] See Figure 2 、 Figure 4 、 Figure 6 The sample placement detection device 3 is composed of a diffuse reflection sensor group 30, a detection vertical movement module 32, and a detection tightening rubber block 33;

[0168] The diffuse reflection sensor group 30 and the detection and tightening rubber block 33 are sequentially arranged at the output end of the detection vertical movement module 32;

[0169] The detection vertical movement module 32 is provided with a detection vertical movement motor 321;

[0170] The sample placement detection device 3 is provided with a detection vertical moving module 32 on the sample placement detection device seat 134 on the bottom surface of the second layer of the partition 13 , and the detection tightening rubber block 33 corresponds to the sample position hole 231 on the centrifugal disk 23 .

[0171] See Figure 1 、 Figure 2 、 Figure 11 、 Figure 12 、 Figure 13 、 Figure 15 The reagent adding device 7 is composed of several reagent bottles 70, a reagent bottle base 71 and a liquid level sensor 72;

[0172] The reagent bottle 70 is provided with a liquid level sensor 72 , and the reagent bottle 70 is disposed on the bottom plate 15 inside the reagent storage door 16 via a reagent bottle base 71 .

[0173] See Figure 2 、 Figure 3 、 Figure 4 、 Figure 13 The cleaning and discarding supernatant device 4 is composed of a cleaning and waste liquid suction component 41, a cleaning and tightening component 42 and a clean water gear pump 43;

[0174] The cleaning and waste liquid suction assembly 41 is composed of a supernatant liquid suction needle assembly 46, a cleaning two-dimensional movable module 48, a waste liquid suction electromagnetic valve assembly 44 and a supernatant liquid discarding waste liquid tank 49;

[0175] The supernatant aspirating needle group 46 is provided at the output end of the cleaning two-dimensional moving module 48;

[0176] The supernatant aspirating needle tube group 46 is provided with a plurality of independently controlled supernatant aspirating needles 461;

[0177] The waste liquid suction solenoid valve group 44 is provided with a plurality of waste liquid suction solenoid valves 441;

[0178] The supernatant sample aspirating needle 461 is connected to the waste liquid aspirating electromagnetic valve 441;

[0179] The cleaning two-dimensional moving module 48 is provided with a cleaning horizontal moving motor 481 and a cleaning vertical moving motor 482;

[0180] The cleaning and waste liquid suction component 41 of the cleaning and supernatant discarding device 4 is arranged on the cleaning and supernatant discarding device seat 122 of a layer of the partition 12 through the cleaning two-dimensional moving module 48;

[0181] The supernatant liquid discarding tank 49 is arranged on the supernatant liquid discarding tank seat 125 of a layer of partition 12.

[0182] See Figure 2 、 Figure 4 、 Figure 7 and Figure 13 The cleaning and tightening assembly 42 is composed of a cleaning and tightening vertical moving module 421 and a cleaning and tightening rubber block 423;

[0183] The cleaning and tightening rubber block 423 is provided at the output end of the cleaning and tightening vertical moving module 421;

[0184] The cleaning and tightening vertical moving module 421 is provided with a cleaning and tightening vertical moving motor 4211;

[0185] The cleaning and tightening assembly 42 is arranged on the sample tightening device seat 136 on the bottom surface of the second-layer partition 13 through the cleaning and tightening vertical moving module 421, and the cleaning and tightening rubber block 423 corresponds to the sample position hole 231 on the centrifugal disk 23;

[0186] The clean water gear pump 43 is connected between the supernatant waste tank 49 and the reagent bottle 70 and is disposed on the bottom surface of the three-layer partition 14 .

[0187] See Figure 2 、 Figure 4 、 Figure 8 The sample vibration device 8 is composed of a vibration vertical movement module 81, a vibration servo platform 82, a vibration rubber block 821, an eccentric disk 803, a vibration servo motor 800 and a vibration shaft 804;

[0188] The vibration servo platform 82 is provided at the output end of the vibration vertical movement module 81;

[0189] The vibration shaft 804, eccentric disk 803 and vibration rubber block 821 are sequentially arranged at the output end of the vibration servo motor 800;

[0190] The vibration vertical movement module 81 is provided with a vibration vertical movement motor 811;

[0191] The vibration servo motor 800 is provided on the vibration servo platform 82;

[0192] The sample vibrating device 8 is disposed on the sample vibrating device seat 135 on the bottom surface of the second partition plate 13 via the vibrating vertical moving module 81 , and the vibrating rubber block 821 corresponds to the sample station hole 231 on the centrifugal disk 23 .

[0193] See Figure 2 、 Figure 9 The constant temperature box inner chamber device 9 is composed of a constant temperature box inner chamber heating cavity 90, a working chamber upper cover 91, a sample placement cover 92 and a door opening and closing sensor 93;

[0194] The working chamber cover 91 is provided on the top of the heating chamber 90 in the constant temperature box, the sample placement cover 92 is provided on the working chamber cover 91, and the door opening and closing sensor 93 is provided directly below the sample placement cover 92, between the sample placement cover 92 and the sample centrifuge rotor assembly 27;

[0195] The constant temperature box inner chamber device 9 is arranged on the constant temperature box inner chamber heating cavity seat 133 of the second layer of the partition 13 through the constant temperature box inner chamber heating cavity 90.

[0196] See Figure 2 、 Figure 9 Furthermore, the heating cavity 90 in the constant temperature box is used to heat the air in the constant temperature box, and cooperate with the insulation effect of the insulation layer in the constant temperature box to ensure that the air temperature in the constant temperature box can meet the temperature conditions required during operation. The air temperature in the constant temperature box is monitored by the temperature sensor in the constant temperature box.

[0197] See Figure 2 、 Figure 3 、 Figure 10 The reagent adding device 6 is composed of a top liquid injection component 60, a hypotonic solution adding component 61, a fixative solution adding component 62 and a fixative solution-hypotonic solution waste liquid tank 64.

[0198] See Figure 10 The top injection assembly 60 is composed of a reagent vertical movement module 600, a reagent rotation motor 601, a fixed solution sample injection needle group 603, a hypotonic solution sample injection needle group 604, and a reagent injection system suspension member 602;

[0199] The fixative solution sample injection needle group 603 and the hypotonic solution sample injection needle group 604 are respectively arranged on both sides of the reagent injection system suspension member 602; the fixative solution sample injection needle group 603 is composed of several independently controlled fixative solution sample injection needles 6031;

[0200] The hypotonic solution sampling needle tube set 604 is composed of several independently controlled hypotonic solution sampling needles 6041;

[0201] The reagent rotating motor 601 is provided at the output end of the reagent vertical moving module 600 , and the reagent injection system suspension member 602 is provided at the output end of the reagent rotating motor 601 ;

[0202] The reagent vertical movement module 600 is provided with a reagent vertical movement motor 6001;

[0203] The top liquid injection assembly 60 is disposed on the reagent adding device seat 112 of the top plate 11 via the reagent vertical moving module 600 .

[0204] See Figure 1 、 Figure 2 、 Figure 3 、 Figure 11 、 Figure 15 The hypotonic solution adding assembly 61 is composed of a hypotonic solution solenoid valve assembly 610, a hypotonic solution infusion tube assembly 611, a hypotonic solution heating bottle 612, a hypotonic solution inlet solenoid valve 614, a hypotonic solution inlet / outlet solenoid valve 615 and a hypotonic solution gear pump 616;

[0205] The hypotonic solution adding component 61 is divided into a hypotonic solution bottle inlet circuit and a hypotonic solution bottle outlet circuit;

[0206] First, the hypotonic solution bottle inlet circuit is connected by a pipeline from the reagent bottle 70 of the reagent adding device 7 through the hypotonic solution-inlet / outlet electromagnetic valve 615, the hypotonic solution gear pump 616, the hypotonic solution-inlet electromagnetic valve 614 to the hypotonic solution heating bottle 612;

[0207] Second, the hypotonic solution bottle outlet circuit is connected via the hypotonic solution heating bottle 612 through the hypotonic solution inlet / outlet electromagnetic valve 615, the hypotonic solution gear pump 616, the hypotonic solution electromagnetic valve assembly 610, the hypotonic solution infusion tube assembly 611 to the hypotonic solution sample injection needle assembly 604 of the top injection assembly 60;

[0208] The hypotonic liquid heating bottle 612 is provided with a hypotonic liquid bottle heater 613 and a hypotonic liquid heating bottle insulation layer 617;

[0209] The hypotonic solution adding assembly 61 is provided on the bottom plate 15 inside the reagent storage compartment door 16;

[0210] The stationary solution-hypotonic solution waste liquid tank 64 is disposed on the stationary solution-hypotonic solution waste liquid tank seat 124 of a layer of the partition plate 12 .

[0211] See Figure 1 、 Figure 2 、 Figure 3 、 Figure 12 、 Figure 15The fixing liquid adding component 62 is composed of a fixing liquid solenoid valve group 620, a fixing liquid infusion tube group 621, a mixer motor moving module 622, a mixed liquid syringe 623, a mixer-methanol solenoid valve 631, a mixer-acetic acid solenoid valve 632 and a mixer flow channel 630;

[0212] The fixing liquid inlet circuit of the fixing liquid adding component 62 is divided into two paths;

[0213] First, adding methanol: the methanol reagent bottle 70 of the reagent adding device 7 is connected through the mixer-methanol solenoid valve 631 and the mixer flow channel 630 to the mixed liquid syringe 623;

[0214] Second, add acetic acid: connect the acetic acid reagent bottle 70 of the reagent adding device 7 through the mixer-acetic acid solenoid valve 632 and the mixer flow channel 630 to the mixed liquid injector 623;

[0215] The methanol and acetic acid reagents are mixed in the mixed liquid syringe 623;

[0216] The fixing liquid is added to the fixing liquid outlet circuit of the component 62:

[0217] The mixed liquid syringe 623 is connected through the mixer flow channel 630, the fixative liquid solenoid valve assembly 620, the fixative liquid infusion tube assembly 621 to the fixative liquid sample injection needle assembly 603 of the top injection assembly 60;

[0218] The fixing solution adding assembly 62 is disposed on the bottom plate 15 inside the reagent storage compartment door 16 .

[0219] See Figure 2 、 Figure 3 、 Figure 13 、 Figure 15 The waste liquid treatment device 5 is composed of a waste liquid diaphragm pump 50, a waste liquid barrel 51, a waste liquid barrel filling solenoid valve 53, a waste liquid barrel-cleaning solenoid valve 54 and a waste liquid barrel-fixed liquid / hypotonic liquid solenoid valve 55:

[0220] The waste liquid recovery circuit of the waste liquid treatment device 5 is divided into two circuits:

[0221] First, the supernatant liquid waste tank 49 on the cleaning and waste liquid suction assembly 41 is connected through the waste liquid barrel-cleaning solenoid valve 54, the waste liquid diaphragm pump 50, the waste liquid barrel filling solenoid valve 53 to the waste liquid barrel 51;

[0222] Secondly, the reagent adding device 6 is connected to the waste liquid tank 51 through the waste liquid barrel-fixative liquid / hypotonic liquid electromagnetic valve 55, the waste liquid diaphragm pump 50, and the waste liquid barrel filling electromagnetic valve 53;

[0223] The waste liquid treatment device 5 is disposed on a bottom plate 15 inside the reagent storage compartment door 16 .

[0224] See Figure 1 、 Figure 2 The control device 10 is a PLC programmable controller, and is electrically connected to the touch screen 101 on the instrument case 1, the centrifugal servo control device 2, the sample placement detection device 3, the cleaning and supernatant discarding device 4, the waste liquid treatment device 5, the reagent adding device 6, the reagent storage device 7, the sample vibration device 8 and the electrical parts of the constant temperature chamber device 9; the control device 10 is arranged on the three-layer partition 14.

[0225] See Figure 2 、 Figure 4 、 Figure 5 、 Figure 14 The sample centrifugal rotor assembly 27 is composed of a sample tube rack mounting base 270, a centrifugal system limit block 271, a sample tube rack cover 272, a sample tube rack bracket 273, a sample tube rack 274, a vibrating pendulum rod 275, a test tube 276 and a vibrating pendulum rubber block 277;

[0226] The sample tube rack 274 and the sample tube rack cover 272 are sequentially arranged at the top of the vibrating pendulum rod 275, the vibrating pendulum rubber block 277 is arranged at the tail end of the vibrating pendulum rod 275, and the test tube 276 is placed in the sample tube rack 274;

[0227] The sample tube rack support 273 of the sample centrifugal rotor assembly 27 is placed in the sample station hole 231 of the centrifugal disk 23 and is connected to the centrifugal disk 23 via the sample tube rack mounting seat 270 .

[0228] Example

[0229] This embodiment uses human peripheral blood as an example and combines the system of the present invention to implement fully automatic chromosome sample harvesting. The specific steps are as follows:

[0230] a. Consumables placement steps: Before the system starts working, clear the waste liquid left in the waste liquid barrel 51 to ensure that the corresponding reagent capacity in the reagent bottle 70 is sufficient.

[0231] b. Device reset step: After executing step a, start the system and operate the control device 10 to reset the device.

[0232] c. Reagent detection step: After executing step b, read the liquid level sensors 72 of several reagents to confirm that the reagent capacity is sufficient.

[0233] d. Leftover test tube detection step: After executing step c, the test tube 276 that may be left in the sample centrifuge rotor assembly 27 is detected; first, the control device 10 drives the centrifugal servo motor 20 to move the sample centrifuge rotor assembly 27 on the centrifugal disk 23 to the test tube insertion position;

[0234] Then, the detection vertical movement motor 321 of the sample placement detection device 3 is driven to raise the detection pressing rubber block 33 to the working position. At this time, the sample centrifugal rotor assembly 27 has entered the detection range of the diffuse reflection sensor group 30. By emitting laser light and receiving reflected light, the distance between the output end of the diffuse reflection sensor 301 and the obstacle is calculated. This distance is compared with the detection range of the diffuse reflection sensor 301 to determine whether there is a test tube 276 left in its detection range.

[0235] If it is detected that a test tube 276 is left on the sample centrifuge rotor assembly 27, the touch screen 101 displays the location of the left test tube 276 and prompts the user to remove the test tube 276. After confirming that there is no test tube 276, the detection and tightening rubber block 33 of the sample placement detection device 3 is reset, and the centrifugal servo motor 20 is controlled to move the next set of sample centrifuge rotor assemblies 27 to the test tube placement position. The detection vertical movement motor 321 of the sample placement detection device 3 is driven again to raise the detection and tightening rubber block 33 to the working position, and the diffuse reflection sensor group 30 detects the sample centrifuge rotor assembly 27.

[0236] The above detection steps are repeated until all the sample centrifuge rotor assemblies 27 are tested.

[0237] e. Test tube placement step: After completing step d, the test tubes 276 are placed; the user enters the number of test tubes 276 to be placed on the touch screen 101, and then, according to the system prompts, places the test tubes 276 containing counterweights or samples in the position of the sample tube rack 274 specified by the sample centrifuge rotor assembly 27.

[0238] f. Centrifugation step: After executing step e, the test tube 276 containing the weight or sample is subjected to high-speed centrifugation. The control device 10 drives the centrifugal servo motor 20 to rotate at 1400 RPM for 9 minutes, thereby separating the cells in the test tube 276 containing the sample from other substances.

[0239] g. Supernatant Discarding Step: After completing step f, the supernatant liquid in the test tube 276 containing the counterweight or sample after centrifugation needs to be aspirated. The control device 10 drives the centrifugal servo motor 20 to move the sample centrifuge rotor assembly 27 on the centrifugal disk 23 to the supernatant discarding position. The control device 10 then drives the cleaning and tightening vertical movement motor 4211 on the cleaning and tightening vertical movement module 421 to press the cleaning and tightening rubber block 423 against the vibrating pendulum rubber block 277 of the sample centrifuge rotor assembly 27.

[0240] Subsequently, the cleaning horizontal moving motor 481 on the cleaning two-dimensional moving module 48 is driven to move the supernatant aspirating needle tube assembly 46 to the supernatant discarding position;

[0241] At this time, the cleaning vertical movement motor 482 on the cleaning two-dimensional movement module 48 is driven to move the supernatant aspirating needle 461 of the supernatant aspirating needle tube assembly 46 downward into the test tube 276 of the sample centrifugal rotor assembly 27 into which the counterweight or sample is placed;

[0242] Open the waste liquid diaphragm pump 50 and the corresponding waste liquid suction solenoid valve 44, and then slowly drive the cleaning vertical movement motor 482 on the cleaning two-dimensional movement module 48 to drive the supernatant aspirating needle 461 to move upward to the end position of discarding the supernatant;

[0243] After the aspiration is completed, the cleaning vertical moving motor 482 is reset, and the waste liquid diaphragm pump 50 and the corresponding waste liquid aspiration solenoid valve 44 are closed. Then, the cleaning and tightening vertical moving motor 4211 is reset, thereby completing the process of discarding the supernatant of all the test tubes 276 with weights or samples placed on the single sample centrifuge rotor assembly 27;

[0244] Then, the centrifugal servo motor 20 is controlled to move the next set of sample centrifugal rotor assemblies 27 to the supernatant discarding position, and the cleaning and tightening vertical movement motor 4211 on the cleaning and tightening vertical movement module 421 is driven again to make the cleaning and tightening rubber block 423 press against the vibrating pendulum rubber block 277 of the sample centrifugal rotor assembly 27;

[0245] The above steps are repeated until the supernatant discarding operation is completed for all the test tubes 276 with weights or samples placed in them on the sample centrifuge rotor assembly 27 .

[0246] h. Needle cleaning step: After executing step g, the supernatant aspiration needle assembly 46 needs to be cleaned to prevent the supernatant waste liquid aspirated in step g from remaining on the supernatant aspiration needle 461; the control device 10 drives the cleaning horizontal movement motor 481 on the cleaning two-dimensional movement module 48 to move the supernatant aspiration needle assembly 46 to a horizontal cleaning position. At this time, the supernatant aspiration needle assembly 46 is located directly above the supernatant waste liquid discarding tank 49;

[0247] Subsequently, the control device 10 drives the cleaning vertical movement motor 482 on the cleaning two-dimensional movement module 48 to move downward, moving the supernatant aspirating needle tube assembly 46 to the vertical cleaning position. At this time, the supernatant aspirating needle 461 has been inserted into the supernatant waste tank 49.

[0248] Subsequently, the control device 10 drives the clean water gear pump 43 to start working, and injects clean water into the supernatant waste tank 49;

[0249] When the clean water in the waste liquid tank 49 reaches a certain height, the clean water gear pump 43 is turned off;

[0250] Subsequently, the waste liquid diaphragm pump 50 and all the waste liquid suction solenoid valves 44 are opened. The waste liquid diaphragm pump 50 draws water, causing it to flow through the supernatant liquid aspirating needle 461 and the corresponding waste liquid aspirating solenoid valve 44, and then through the waste liquid diaphragm pump 50 and the waste liquid barrel dispensing solenoid valve 53, and finally flows into the waste liquid barrel 51, thereby completing a single cleaning process of the supernatant liquid aspirating needle tube set 46;

[0251] After completing a single cleaning, the waste liquid diaphragm pump 50 and all waste liquid suction solenoid valves 44 are closed; then, the waste liquid bucket-cleaning solenoid valve 54 is opened and the waste liquid diaphragm pump 50 is opened again to drain the liquid remaining in the discarded supernatant liquid waste tank 49 after the needle tube is cleaned; after draining, the clean water gear pump 43 is controlled to work again to inject clean water into the discarded supernatant liquid waste tank 49;

[0252] After repeating the above steps for a certain number of times, the cleaning vertical moving motor 482 on the cleaning two-dimensional moving module 48 is reset to complete the cleaning process of the supernatant aspirating needle tube group 46.

[0253] i. Sedimentation and Shaking Step: After completing step h, the sample centrifuge rotor assembly 27 containing the counterweight or sample in the test tube 276 needs to be sedimented and shaken. The control device 10 drives the centrifugal servo motor 20 to move the sample centrifuge rotor assembly 27 on the centrifugal disk 23 to the sample vibration position. The control device 10 then drives the vibration vertical movement motor 811 on the vibration vertical movement module 81 to cause the vibration rubber block 82 to contact the vibration pendulum rubber block 277 of the sample centrifuge rotor assembly 27.

[0254] At this time, the vibration servo motor 800 is driven to move, and the vibration servo motor 800 drives the vibration rubber block 82 and the test tube 276 with the counterweight or sample placed on the sample centrifugal rotor assembly 27 to vibrate through the vibration shaft 804 and the eccentric disk 803;

[0255] After the vibration ends, the vibration vertical movement module 81 is reset;

[0256] Subsequently, the control device 10 drives the centrifugal servo motor 20 to move, bringing the next group of sample centrifuge rotor assemblies 27 on the centrifugal disk 23 to the sample shaking position, and repeats the sedimentation and shaking process until the sedimentation and shaking operation of the test tubes 276 with counterweights or samples placed on all sample centrifuge rotor assemblies 27 is completed.

[0257] j. Hypotonic treatment step: After executing step i, the test tube 276 containing the weight or sample is subjected to hypotonic treatment by adding hypotonic solution, thereby causing the sample cells to swell, the chromosome sample to disperse, and the stretching and separation process to be completed. Since the hypotonic treatment of the sample must be performed at a constant temperature of 37°C, it is necessary to ensure that the temperature of the heating chamber 90 in the constant temperature chamber has reached 37°C before adding the hypotonic solution. The control device 10 reads the value of the temperature sensor in the constant temperature chamber to confirm whether the internal temperature of the heating chamber 90 in the constant temperature chamber has reached 37°C. If not, subsequent operations will be carried out after the temperature condition is met. If it has reached 37°C, subsequent operations can be started directly.

[0258] After confirming that the temperature conditions are met, for each hypotonic treatment operation of the test tube 276 containing the counterweight or sample on the centrifugal rotor assembly 27, the hypotonic solution in the hypotonic solution heating bottle 612 is replenished to the highest point before each round.

[0259] Then, the control device 10 drives the centrifugal servo motor 20 to move the sample centrifugal rotor assembly 27 on the centrifugal disk 23 to the position for adding hypotonic fluid; then, the control device 10 drives the vibration vertical movement motor 811 on the vibration vertical movement module 81 to make the vibration rubber block 82 and the vibration pendulum rubber block 277 of the sample centrifugal rotor assembly 27 press against each other;

[0260] Subsequently, the reagent rotation motor 601 is controlled to move the hypotonic solution sample injection needle tube assembly 604 to the waste liquid discharge position. At this time, the hypotonic solution sample injection needle tube assembly 604 is located directly above the fixative-hypotonic solution waste liquid tank 64. Then, the reagent vertical movement motor 6001 on the reagent vertical movement module 600 is moved downward to the vertical waste liquid discharge position. At this time, the hypotonic solution sample injection needle 6041 has been inserted into the fixative-hypotonic solution waste liquid tank 64.

[0261] Then, the hypotonic liquid gear pump 616, the hypotonic liquid inlet / outlet solenoid valve 615, and all the hypotonic liquid solenoid valves 610 are opened to discharge the cooled hypotonic liquid at the front end of the hypotonic liquid sampling needle 6041. At the same time, the waste liquid bucket-fixative liquid / hypotonic liquid solenoid valve 55 and the waste liquid diaphragm pump 50 are also opened to pump the discharged cooled hypotonic liquid into the waste liquid bucket 51 through the waste liquid bucket dispensing solenoid valve 53.

[0262] After all the cooled hypotonic fluid at the front end of the hypotonic fluid sampling needle 6041 is discharged, the reagent vertical movement motor 6001 on the reagent vertical movement module 600 is reset;

[0263] Then, the reagent rotating motor 601 is driven to move the hypotonic solution adding needle tube assembly 604 to the hypotonic solution adding position, and then the reagent vertical moving motor 6001 on the reagent vertical moving module 600 is controlled to move downward to the hypotonic solution adding position in the vertical direction. At this time, the hypotonic solution adding needle tube assembly 604 is located directly above the sample centrifuge rotor assembly 27, and the hypotonic solution adding needle 6041 has been inserted into the corresponding test tube 276 containing the counterweight or sample.

[0264] At this time, the hypotonic fluid gear pump 616 and the hypotonic fluid solenoid valve 610 containing the test tube 276 containing the counterweight or sample are opened, and 8 ml of hypotonic fluid at a temperature of 37° C. is injected. After confirming through sensor feedback that the volume of hypotonic fluid added to the test tube 276 containing the counterweight or sample has reached 8 ml, the relevant solenoid valve is closed; and the reagent vertical movement motor 6001 on the vertical movement module 600 is reset.

[0265] Then, the precipitation and shaking process in step i is performed to complete the hypotonic treatment operation of all the test tubes 276 containing weights or samples on the single sample centrifuge rotor assembly 2;

[0266] Next, the control device 10 drives the centrifugal servo motor 20 to move, bringing the next set of sample centrifuge rotor assemblies 27 on the centrifugal disk 23 to the position for adding hypotonic solution, and repeating the above steps to complete the hypotonic treatment operation for the test tubes 276 with weights or samples placed on all sample centrifuge rotor assemblies 27;

[0267] After all the test tubes 276 with weights or samples placed therein have completed the hypotonic treatment operation, a constant temperature of 37°C is maintained for 30 minutes. After the static condition is completed, the heater (900) in the constant temperature box is turned off to complete the hypotonic treatment process for all the test tubes 276 with weights or samples placed therein.

[0268] k. Fixation step: After completing step j, all test tubes 276 containing weights or samples are fixed with a fixative to stabilize the structure of the chromosome sample and facilitate subsequent staining, dehydration, and clearing. Furthermore, because the sample may contain a large number of cells or a complex structure, multiple fixation steps may be required to ensure that the fixation requirements at different stages are met, thereby achieving the desired fixation effect.

[0269] Since fresh fixative is used during the chromosome sample harvesting process, it is necessary to check whether the fixative is freshly prepared on the same day when it is used for the first time on the same day. If it is, the subsequent operation will continue. If not, the fixative is replaced with the freshly prepared fixative on the same day and then the subsequent operation will be carried out. After confirming that the fixative is freshly prepared on the same day, the test tube 276 containing the counterweight or sample on each sample centrifuge rotor assembly 27 is fixed.

[0270] Before each round of the fixation process of each test tube 276 containing weights or samples on the centrifuge rotor assembly 27, the control device 10 calculates the total volume of fixative required for all test tubes 276 containing weights or samples in the current round and compares it with the current volume of fixative. If the volume is sufficient, the process of drawing methanol and acetic acid for the fixative is skipped. If the volume is insufficient, the corresponding volume of methanol and acetic acid is drawn according to the difference in volume for the fixative.

[0271] When the total volume of the stationary solution is insufficient, the mixer-acetic acid solenoid valve 632 is first opened, and the mixer motor 6220 is controlled to move a certain number of steps to draw a certain volume of acetic acid reagent into the mixed solution syringe 623. The mixer-acetic acid solenoid valve 632 is then closed. The mixer-methanol solenoid valve 631 is then opened, and the mixer motor 6220 is controlled to move a certain number of steps to draw a certain volume of methanol reagent into the mixed solution syringe 623. The mixer-methanol solenoid valve 631 is then closed. The two reagents are mixed in the mixed solution syringe 623, thereby completing the preparation of the required volume of stationary solution.

[0272] When the total volume of the fixative solution is sufficient, the control device 10 drives the centrifugal servo motor 20 to move the sample centrifugal rotor assembly 27 on the centrifugal disk 23 to the position where the fixative solution is added; then the control device 10 drives the vibration vertical movement motor 811 on the vibration vertical movement module 81 to make the vibration rubber block 82 contact the vibration pendulum rubber block 277 of the sample centrifugal rotor assembly 27;

[0273] Next, the reagent rotation motor 601 is controlled to move the fixative solution injection needle assembly 603 to the fixative solution injection position, and then the reagent vertical movement motor 6001 on the reagent vertical movement module 600 is controlled to move downward to the fixative solution injection position in the vertical direction. At this time, the fixative solution injection needle assembly 603 is located directly above the sample centrifuge rotor assembly 27, and the fixative solution injection needle 6031 has been inserted into the corresponding test tube 276 containing the counterweight or sample.

[0274] Then, the fixative solenoid valve 620 corresponding to the number of the test tube 276 containing the counterweight or sample is opened, and the mixer motor 6220 is operated according to the sample volume to complete the operation of adding the fixative to all the test tubes 276 containing the counterweight or sample on the single sample centrifuge rotor assembly 27;

[0275] Subsequently, the corresponding fixative solenoid valve 620 is closed, and the reagent vertical movement motor 6001 on the reagent vertical movement module 600 is reset; then, step i is performed to shake the sedimentation well to accelerate the diffusion of the fixative and allow it to fully function, thereby completing the fixation operation of all the test tubes 276 containing the counterweights or samples on the single sample centrifuge rotor assembly 27;

[0276] After the fixation operation is repeated for all test tubes 276 containing weights or samples on the sample centrifuge rotor assembly 27, step f, centrifugation, step g, discarding the supernatant, and step h, needle cleaning are performed. After completing steps f, g, and h, step i, sedimentation and shaking, is performed again, thereby completing the first fixation process.

[0277] After the first fixation process is completed, there are second and third fixation processes. Different fixation processes are designed to meet the fixation requirements at different stages, thereby achieving the ideal fixation effect for the chromosome sample.

[0278] The second and third fixation processes are basically the same as the first fixation process. The difference is that in the second and third fixation processes, after all the weights on the sample centrifuge rotor assembly 27 are placed or the fixative solution is added to the sample tubes, it is necessary to stand for 20 minutes to complete the fixation process of the chromosome sample. The remaining steps are also consistent with the first fixation process.

[0279] 1. Test tube removal step: After completing step k, all test tubes 276 containing weights or samples are removed. The chromosome samples obtained from the test tubes 276 containing samples can be used to prepare subsequent chromosome sample slides for observation under a microscope and karyotype analysis.

[0280] The control device 10 drives the centrifugal servo motor 20 to move the sample centrifuge rotor assembly 27 on the centrifuge disk 23 to the test tube insertion position. The touch screen 101 then prompts the user to remove all test tubes 276 containing weights or samples placed on the current sample centrifuge rotor assembly 27. After the removal is completed, the user presses the confirmation button on the touch screen 101.

[0281] After the user presses the confirmation button, the control device 10 reads the diffuse reflection sensor group 30 to perform test tube detection. After confirming that the test tube 276 with the inserted weight or sample placed on the current sample centrifuge rotor assembly 27 has been completely removed, the centrifugal servo motor 20 is driven to move, and the next set of sample centrifuge rotor assemblies 27 on the centrifugal disk 23 is brought to the test tube insertion position. The above test tube removal process is repeated until the inserted weight or sample test tube 276 on all sample centrifuge rotor assemblies 27 are removed, completing the test tube removal step.

[0282] At this point, the chromosome sample harvesting process is completed, and the harvested chromosome samples can be directly used for the subsequent preparation of chromosome sample slides.

[0283] The present invention transforms the chromosome sample harvesting process, which currently requires manual operation by an operator, into a fully automated system, reducing the labor and time costs of chromosome sample harvesting. While improving chromosome sample harvesting efficiency and ensuring the quality and consistency of harvested samples, it also reduces the possibility of infection for operators and avoids the generation of nosocomial contamination. The above describes the basic principles, main features, and advantages of the embodiments of the present invention.

[0284] Finally, it should be noted that the above embodiment is only a preferred embodiment of the present invention, and the present invention is not limited to the above specific embodiments. Those skilled in the art to which the present invention belongs can also make changes and modifications to the above embodiments. Those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent substitutions for some of the technical features therein, but should be noted that they cannot deviate from the purpose, principle and scope of the technical solution of the present invention, and any modifications, replacements, improvements, etc. made should be included in the scope of protection of the present invention and the scope of the claims.

Claims

1. A fully automatic chromosome sample harvesting system, characterized in that: The system comprises an instrument housing (1), a centrifugal servo control device (2), a sample placement detection device (3), a cleaning and supernatant discarding device (4), a waste liquid treatment device (5), a reagent adding device (6), a reagent storage device (7), a sample vibration device (8), a constant temperature chamber inner compartment device (9) and a control device (10); The instrument box (1) is a hexahedron, and is provided with a top plate (11) and a bottom plate (15). A first-layer partition (12), a second-layer partition (13) and a third-layer partition (14) are sequentially provided in the box from top to bottom. A touch screen (101) is provided on the surface, an operating cabin cover (19) is provided between the top plate (11) and the first-layer partition (12), and a reagent storage door (16) is provided between the third-layer partition (14) and the bottom plate (15); an ultraviolet lamp (111), a reagent adding device seat (112), an illuminating lamp (113) and a filter (117) are provided on the bottom surface of the top plate (11), and a heat dissipation device (118) is provided on the outside of the top surface that is connected to the filter (117); The first layer of the partition plate (12) is provided with a turntable hole (121), and a cleaning and supernatant discarding device seat (122), a stationary liquid-hypotonic liquid waste tank seat (124) and a supernatant discarding waste tank seat (125) are provided around the turntable hole (121); A centrifugal disc shaft seat (131) is provided at the center of the second-layer partition (13), a constant temperature box inner chamber heating cavity seat (133) is provided on the periphery, and a centrifugal servo motor seat (132) is provided on one side; The bottom surface of the second-layer partition (13) is provided with a sample placement detection device seat (134), a sample vibration device seat (135) and a sample tightening device seat (136); The centrifugal servo control device (2) is composed of a centrifugal servo motor (20), a centrifugal disc (23), a centrifugal shaft (24), a centrifugal bearing seat (25) and a centrifugal transmission structure (26); the centrifugal servo motor (20) is arranged on a centrifugal servo motor seat (132) on the surface of the second-layer partition (13); the centrifugal transmission structure (26) is arranged on the bottom surface of the second-layer partition (13); the centrifugal bearing seat (25) is arranged on the centrifugal disc shaft seat (131) on the surface of the second-layer partition (13); the centrifugal disc (23) is located in a turntable hole (121) on the first-layer partition (12); The sample placement detection device (3) is composed of a diffuse reflection sensor group (30), a detection vertical movement module (32), and a detection tightening rubber block (33); The diffuse reflection sensor group (30) and the detection tightening rubber block (33) are sequentially arranged at the output end of the detection vertical movement module (32); The detection vertical movement module (32) is provided with a detection vertical movement motor (321); The sample placement detection device (3) is arranged on the sample placement detection device seat (134) on the bottom surface of the second-layer partition (13) through the detection vertical moving module (32), and the detection tightening rubber block (33) corresponds to the sample position hole (231) on the centrifugal disk (23); The reagent adding device (7) is composed of reagent bottles (70) of several reagents, a reagent bottle base (71) and a liquid level sensor (72); The reagent bottle (70) is provided with a liquid level sensor (72), and the reagent bottle (70) is arranged on the bottom plate (15) inside the reagent storage door (16) via a reagent bottle base (71); The cleaning and supernatant liquid discarding device (4) is composed of a cleaning and waste liquid suction component (41), a cleaning and tightening component (42) and a clean water gear pump (43); The cleaning and waste liquid aspirating assembly (41) is composed of a supernatant liquid aspirating needle tube assembly (46), a cleaning two-dimensional movable module (48), a waste liquid aspirating electromagnetic valve assembly (44) and a supernatant liquid discarding waste liquid tank (49); The cleaning and waste liquid suction component (41) of the cleaning and supernatant liquid discarding device (4) is arranged on the cleaning and supernatant liquid discarding device seat (122) of a layer of partition (12) via a cleaning two-dimensional movable module (48); The supernatant liquid waste tank (49) is arranged on a supernatant liquid waste tank seat (125) of a layer of partition (12); The clean water gear pump (43) is arranged on the bottom surface of the three-layer partition plate (14); The sample vibration device (8) is composed of a vibration vertical movement module (81), a vibration servo platform (82), a vibration rubber block (821), an eccentric disk (803), a vibration servo motor (800) and a vibration shaft (804); the sample vibration device (8) is arranged on a sample vibration device seat (135) on the bottom surface of the second-layer partition (13) via the vibration vertical movement module (81), and the vibration rubber block (821) corresponds to the sample station hole (231) on the centrifugal disk (23); The constant temperature box inner chamber device (9) is composed of a constant temperature box inner chamber heating cavity (90), a working chamber upper cover (91), a sample placement cover (92) and a door opening / closing sensor (93); The working chamber upper cover (91) is arranged on the top of the heating chamber (90) in the constant temperature box, the sample placement cover (92) is arranged on the working chamber upper cover (91), and the door opening and closing sensor (93) is arranged directly below the sample placement cover (92) and between the sample placement cover (92) and the sample centrifuge rotor assembly (27); The constant temperature box inner chamber device (9) is arranged on the constant temperature box inner chamber heating cavity seat (133) of the second layer of the partition (13) via the constant temperature box inner chamber heating cavity (90); The reagent adding device (6) is composed of a top liquid injection component (60), a hypotonic solution adding component (61), a fixative solution adding component (62) and a fixative solution-hypotonic solution waste liquid tank (64); The top liquid injection assembly (60) is arranged on the reagent adding device seat (112) of the top plate (11) via the reagent vertical moving module (600); The hypotonic liquid adding assembly (61) is arranged on the bottom plate (15) inside the reagent storage door (16); The stationary liquid-hypotonic liquid waste tank (64) is arranged on the stationary liquid-hypotonic liquid waste tank seat (124) of a layer of partition (12); The fixing solution adding assembly (62) is arranged on the bottom plate (15) inside the reagent storage door (16); The waste liquid treatment device (5) is composed of a waste liquid diaphragm pump (50), a waste liquid barrel (51), a waste liquid barrel filling solenoid valve (53), a waste liquid barrel-cleaning solenoid valve (54) and a waste liquid barrel-fixed liquid / hypotonic liquid solenoid valve (55). The waste liquid treatment device (5) is arranged on a bottom plate (15) inside the reagent storage door (16); The control device (10) is a PLC programmable controller, and the control device (10) is electrically connected to the touch screen (101) on the instrument box (1), the centrifugal servo control device (2), the sample placement detection device (3), the cleaning and supernatant discarding device (4), the waste liquid treatment device (5), the reagent adding device (6), the reagent storage device (7), the sample vibration device (8), and the electrical parts of the constant temperature chamber device (9); The control device (10) is arranged on the three-layer partition plate (14).

2. The fully automatic chromosome sample harvesting system according to claim 1, characterized in that: The centrifugal disc (23) of the centrifugal servo control device (2) is coaxially connected to the centrifugal bearing seat (25) via the centrifugal shaft (24); The centrifugal servo motor (20) is connected to the centrifugal shaft (24) via a centrifugal transmission structure (26); The centrifugal disk (23) is provided with a plurality of sample station holes (231) evenly distributed along the circumference, and a sample centrifugal rotor assembly (27) is provided in the sample station hole (231).

3. The fully automatic chromosome sample harvesting system according to claim 2, characterized in that: The sample centrifugal rotor assembly (27) is composed of a sample tube rack mounting seat (270), a centrifugal system limit block (271), a sample tube rack upper cover (272), a sample tube rack support (273), a sample tube rack (274), a vibrating pendulum rod (275), a test tube (276) and a vibrating pendulum rubber block (277); The sample tube rack (274) and the sample tube rack upper cover (272) are sequentially arranged at the top end of the vibrating pendulum rod (275), the vibrating pendulum rubber block (277) is arranged at the tail end of the vibrating pendulum rod (275), and the test tube (276) is placed in the sample tube rack (274); The sample tube rack support (273) of the sample centrifugal rotor assembly (27) is placed in the sample station hole (231) of the centrifugal disk (23) and is connected to the centrifugal disk (23) via the sample tube rack mounting seat (270).

4. The fully automatic chromosome sample harvesting system according to claim 1, characterized in that: The supernatant aspirating needle group (46) of the cleaning and supernatant discarding device (4) is arranged at the output end of the cleaning two-dimensional moving module (48); The supernatant aspirating needle tube group (46) is provided with a plurality of independently controlled supernatant aspirating needles (461); The waste liquid suction solenoid valve group (44) is provided with a plurality of waste liquid suction solenoid valves (441); The supernatant liquid aspirating needle (461) is connected to the waste liquid aspirating electromagnetic valve (441); The cleaning two-dimensional moving module (48) is provided with a cleaning horizontal moving motor (481) and a cleaning vertical moving motor (482); The cleaning and tightening assembly (42) is composed of a cleaning and tightening vertical moving module (421) and a cleaning and tightening rubber block (423); The cleaning and tightening rubber block (423) is arranged at the output end of the cleaning and tightening vertical moving module (421); The cleaning, tightening and vertically moving module (421) is provided with a cleaning, tightening and vertically moving motor (4211); The cleaning and tightening assembly (42) is arranged on the sample tightening device seat (136) on the bottom surface of the second-layer partition (13) through the cleaning and tightening vertical moving module (421), and the cleaning and tightening rubber block (423) corresponds to the sample station hole (231) on the centrifugal disk (23); The clean water gear pump (43) is connected between the supernatant waste tank (49) and the reagent bottle (70).

5. The fully automatic chromosome sample harvesting system according to claim 1, characterized in that: The vibration servo platform (82) of the sample vibration device (8) is arranged at the output end of the vibration vertical movement module (81); The vibration shaft (804), the eccentric disk (803) and the vibration rubber block (821) are sequentially arranged at the output end of the vibration servo motor (800); The vibrating vertical movement module (81) is provided with a vibrating vertical movement motor (811); The vibration servo motor (800) is arranged on the vibration servo platform (82).

6. The fully automatic chromosome sample harvesting system according to claim 1, characterized in that: The top liquid injection assembly (60) of the reagent adding device (6) is composed of a reagent vertical moving module (600), a reagent rotating motor (601), a fixed solution sample injection needle tube group (603), a hypotonic solution sample injection needle tube group (604), and a reagent injection system suspension member (602); The fixed solution sample injection needle tube group (603) and the hypotonic solution sample injection needle tube group (604) are respectively arranged on both sides of the reagent injection system suspension member (602); The fixative solution sample injection needle tube set (603) is composed of several independently controlled fixative solution sample injection needles (6031); The hypotonic solution sampling needle tube set (604) is composed of several independently controlled hypotonic solution sampling needles (6041); The reagent rotating motor (601) is provided at the output end of the reagent vertical moving module (600), and the reagent injection system suspension component (602) is provided at the output end of the reagent rotating motor (601); The reagent vertical movement module (600) is provided with a reagent vertical movement motor (6001); The hypotonic solution adding assembly (61) is composed of a hypotonic solution electromagnetic valve assembly (610), a hypotonic solution infusion tube assembly (611), a hypotonic solution heating bottle (612), a hypotonic solution inlet electromagnetic valve (614), a hypotonic solution inlet / outlet electromagnetic valve (615), and a hypotonic solution gear pump (616); The hypotonic liquid adding component (61) is divided into a hypotonic liquid bottle liquid inlet circuit and a hypotonic liquid bottle liquid outlet circuit; First, the hypotonic solution bottle inlet circuit is connected by a pipeline from the reagent bottle (70) of the reagent adding device (7) through the hypotonic solution-inlet / outlet electromagnetic valve (615), the hypotonic solution gear pump (616), the hypotonic solution-inlet electromagnetic valve (614) to the hypotonic solution heating bottle (612); Second, the hypotonic solution bottle outlet circuit is connected via a hypotonic solution heating bottle (612) through a hypotonic solution inlet / outlet electromagnetic valve (615), a hypotonic solution gear pump (616), a hypotonic solution electromagnetic valve assembly (610), a hypotonic solution infusion tube assembly (611) to a hypotonic solution sample injection needle assembly (604) of a top injection assembly (60); The hypotonic liquid heating bottle (612) is provided with a hypotonic liquid bottle heater (613) and a hypotonic liquid heating bottle insulation layer (617); The fixing liquid adding component (62) is composed of a fixing liquid electromagnetic valve group (620), a fixing liquid infusion tube group (621), a mixer motor moving module (622), a mixed liquid syringe (623), a mixer-methanol electromagnetic valve (631), a mixer-acetic acid electromagnetic valve (632) and a mixer flow channel (630); The fixing liquid inlet circuit of the fixing liquid adding component (62) is divided into two paths; First, adding methanol: connecting the methanol reagent bottle (70) of the reagent adding device (7) through the mixer-methanol solenoid valve (631), the mixer flow channel (630) to the mixed liquid syringe (623); Second, add acetic acid: connect the acetic acid reagent bottle (70) of the reagent adding device (7) through the mixer-acetic acid solenoid valve (632), the mixer flow channel (630) to the mixed liquid injector (623); The methanol and acetic acid reagents are mixed in a mixed liquid syringe (623); The fixative liquid outlet circuit of the fixative addition component (62) is connected by a mixed liquid syringe (623) through a mixer flow channel (630), a fixative liquid solenoid valve assembly (620), a fixative liquid infusion tube assembly (621) to a fixative liquid sample injection needle assembly (603) of the top liquid injection assembly (60).

7. The fully automatic chromosome sample harvesting system according to claim 1, characterized in that: The waste liquid recovery circuit of the waste liquid treatment device (5) is divided into two circuits: First, the supernatant liquid waste tank (49) on the cleaning and waste liquid suction component (41) is connected through a waste liquid barrel-cleaning electromagnetic valve (54), a waste liquid diaphragm pump (50), a waste liquid barrel filling electromagnetic valve (53) to a waste liquid barrel (51) pipeline; Secondly, the reagent adding device (6) is connected to the waste liquid tank (51) by the fixed liquid-hypotonic liquid waste tank (64) through the waste liquid barrel-fixed liquid / hypotonic liquid electromagnetic valve (55), the waste liquid diaphragm pump (50), the waste liquid barrel filling electromagnetic valve (53).

8. A method for fully automatic chromosome sample harvesting, characterized in that: The method implements fully automatic chromosome sample harvesting based on the system of claim 1, specifically comprising the following steps: a. Consumables removal step: Before the system starts working, remove the waste liquid left in the waste barrel (51) to ensure that the corresponding reagent capacity in the reagent bottle (70) is sufficient; b. Device reset step: After executing step a, start the system and operate the control device (10) to reset the device; c. Reagent detection step: After executing step b, read the liquid level sensor (72) of several reagents to confirm that the reagent capacity is sufficient; d. Remaining test tube detection step: After executing step c, the test tube (276) that may be left in the sample centrifugal rotor assembly (27) is detected; the diffuse reflection sensor group (30) of the sample placement detection device (3) determines whether there is a test tube (276) left on the sample centrifugal rotor assembly (27); If it is detected that there is a test tube (276) left on the sample centrifugal rotor assembly (27), the touch screen (101) displays the location of the remaining test tube (276) and prompts to remove the test tube (276); after confirming that there is no test tube (276), the diffuse reflection sensor group (30) detects the next group of sample centrifugal rotor assemblies (27); Repeat the detection steps until all sample centrifuge rotor assemblies (27) are tested; e. Test tube placement step: After completing step d, start placing the test tubes (276); input the number of test tubes (276) to be placed on the touch screen (101), and then, according to the prompts, place the test tubes (276) with weights or samples in the position of the sample tube rack (274) specified by the sample centrifuge rotor assembly (27); f. Centrifugation step: After executing step e, the test tube (276) containing the weight or sample is subjected to high-speed centrifugation; the control device (10) drives the centrifugal servo motor (20) to rotate at a speed of 1000-1400 RPM for 5-10 minutes, thereby separating the cells in the test tube (276) containing the sample from other substances; g. Step of discarding the supernatant: After executing step f, the supernatant is removed from the test tube (276) containing the counterweight or sample after centrifugation; the cleaning top tight vertical moving motor (4211) on the cleaning top tight vertical moving module (421) is driven to press the cleaning top tight rubber block (423) against the vibrating pendulum rubber block (277) of the sample centrifugal rotor assembly (27); Driving the cleaning horizontal moving motor (481) on the cleaning two-dimensional moving module (48) to move the supernatant aspirating needle tube group (46) to a supernatant discarding position; Driving the cleaning vertical moving motor (482) on the cleaning two-dimensional moving module (48) to move the supernatant aspirating needle (461) of the supernatant aspirating needle tube assembly (46) downward into the sample centrifugal rotor assembly (27) and place it into the counterweight or sample test tube (276); Open the waste liquid diaphragm pump (50) and the corresponding waste liquid suction solenoid valve (44), then slowly drive the cleaning vertical movement motor (482) on the cleaning two-dimensional movement module (48) to drive the supernatant aspiration needle (461) to move upward to the end position of discarding the supernatant; After the aspiration is completed, the cleaning vertical moving motor (482) is reset, and the waste liquid diaphragm pump (50) and the corresponding waste liquid aspiration electromagnetic valve (44) are closed, and then the cleaning and tightening vertical moving motor (4211) is reset, thereby completing the process of discarding the supernatant of all the test tubes (276) with counterweights or samples placed on the single sample centrifuge rotor assembly (27); Then, the centrifugal servo motor (20) is controlled to move all the test tubes (276) with weights or samples placed on the next group of sample centrifugal rotor assemblies (27) to the supernatant discarding position to complete the supernatant discarding process; until the supernatant discarding operation is completed for all the test tubes (276) with weights or samples placed on the sample centrifugal rotor assemblies (27); h. Needle cleaning step: After executing step g, clean the supernatant sampling needle group (46) to avoid the supernatant waste liquid absorbed in step g on the supernatant sampling needle (461); move the supernatant sampling needle group (46) to the top of the supernatant waste liquid tank (49); Controlling the supernatant aspirating needle (461) to be inserted into the supernatant waste tank (49); Subsequently, the control device (10) drives the clean water gear pump (43) to start working and inject clean water into the supernatant waste liquid tank (49); After the clean water in the waste liquid tank (49) for discarding the supernatant reaches the set height, the clean water gear pump (43) is turned off; subsequently, the waste liquid diaphragm pump (50) and all the waste liquid suction solenoid valves (44) are opened, and the water is drawn by the waste liquid diaphragm pump (50) so that the water flows through the supernatant sample suction needle (461) and the corresponding waste liquid suction solenoid valve (44), and then flows through the waste liquid diaphragm pump (50) and the waste liquid barrel dispensing solenoid valve (53), and finally flows into the waste liquid barrel (51), thereby completing a single cleaning of the supernatant sample suction needle tube group (46); After completing a single cleaning, pour clean water into the waste liquid tank (49) for discarding the supernatant; After repeating the above steps several times, the cleaning vertical moving motor (482) on the cleaning two-dimensional moving module (48) is reset to complete the cleaning process of the supernatant aspirating needle tube group (46); i. Sedimentation and shaking step: After executing the above step h, the counterweight or sample test tube (276) placed in the sample centrifugal rotor assembly (27) is sedimented and shaken; the vibration vertical movement motor (811) on the vibration vertical movement module (81) is driven to make the vibration rubber block (82) and the vibration pendulum rubber block (277) of the sample centrifugal rotor assembly (27) bear against each other; at this time, the vibration servo motor (800) is driven to move, and the vibration servo motor (800) vibrates together with the counterweight or sample test tube (276) placed on the sample centrifugal rotor assembly (27); After the vibration ends, the vibration vertical movement module (81) is reset; Subsequently, the next set of sample centrifugal rotor assemblies (27) on the centrifugal disk (23) is brought to the sample shaking position, and the sedimentation and shaking process is repeated until the sedimentation and shaking operation of the test tubes (276) with weights or samples placed on all sample centrifugal rotor assemblies (27) is completed; j. Hypotonic treatment step: After executing step i, it is necessary to add hypotonic solution to the test tube (276) containing the weight or sample obtained after treatment to perform hypotonic treatment, so as to expand the sample cells, disperse the chromosome sample, and complete the stretching and separation process; before adding the hypotonic solution, ensure that the temperature of the heating chamber (90) in the constant temperature box has reached 37°C; only after reaching 37°C can subsequent operations be started; For the hypotonic treatment operation of the test tube (276) with the counterweight or sample placed on each sample centrifuge rotor assembly (27), the hypotonic solution in the hypotonic solution heating bottle (612) is first replenished to the highest point before each round starts; Driving the vibration vertical movement motor (811) on the vibration vertical movement module (81) to make the vibration rubber block (82) and the vibration pendulum rubber block (277) of the sample centrifugal rotor assembly (27) press against each other; Then, the reagent rotating motor (601) is controlled to move the hypotonic solution sample injection needle assembly (604) to the waste liquid discharge position, and the hypotonic solution sample injection needle (6041) is inserted into the fixative-hypotonic solution waste liquid tank (64); Then, the hypotonic liquid gear pump (616), the hypotonic liquid-inlet / outlet electromagnetic valve (615) and all the hypotonic liquid electromagnetic valves (610) are opened to discharge the cooled hypotonic liquid at the front end of the hypotonic liquid sampling needle (6041); at the same time, the waste liquid barrel-fixative liquid / hypotonic liquid electromagnetic valve (55) and the waste liquid diaphragm pump (50) are opened to pump the discharged cooled hypotonic liquid into the waste liquid barrel (51) through the waste liquid barrel dispensing electromagnetic valve (53); After all the cooled hypotonic fluid at the front end of the hypotonic fluid sampling needle (6041) is discharged, the reagent vertical movement motor (6001) on the reagent vertical movement module (600) is reset; Then, the hypotonic solution adding needle assembly (604) is driven to move to the hypotonic solution adding position, and the hypotonic solution adding needle (6041) is inserted into the corresponding test tube (276) into which the counterweight or sample is placed; At this time, the hypotonic liquid gear pump (616) and the hypotonic liquid solenoid valve (610) containing the test tube (276) containing the counterweight or sample are opened, and 8 ml of hypotonic liquid at a temperature of 37° C. are injected; after confirming through sensor feedback that the volume of the hypotonic liquid added to the test tube (276) containing the counterweight or sample reaches 8 ml, the relevant solenoid valve is closed; and the reagent vertical movement motor (6001) on the vertical movement module (600) is reset; Then, the precipitation and shaking process of step i is performed to complete the hypotonic treatment operation of all the test tubes (276) containing weights or samples on the single sample centrifuge rotor assembly (27); Next, the next set of sample centrifugal rotor assemblies (27) on the centrifugal disk (23) is brought to the position for adding hypotonic solution, and the above steps are repeated to complete the hypotonic treatment operation of the test tubes (276) with weights or samples placed on all sample centrifugal rotor assemblies (27); k. Fixation treatment step: After performing step j, all the test tubes (276) containing weights or samples are fixed by adding a fixative to stabilize the structure of the chromosome sample, facilitating subsequent staining, dehydration, and clearing treatments; When using for the first time, check whether the fixative is fresh; if so, continue with the subsequent operation; For the fixation process of the test tube (276) with the weight or sample placed on each sample centrifuge rotor assembly (27), before each round begins, the total volume of the fixative required for all the test tubes (276) with the weight or sample placed in this round is calculated and compared with the current volume of the fixative. If the volume is sufficient, the process continues. If the volume is insufficient, the corresponding volume of methanol and acetic acid will be drawn according to the difference for configuration; Under the condition that the total volume of the fixative is sufficient, the sample centrifugal rotor assembly (27) on the centrifugal disk (23) is brought to the position for adding the fixative; then the vibration vertical movement motor (811) on the vibration vertical movement module (81) is driven to make the vibration rubber block (82) and the vibration pendulum rubber block (277) of the sample centrifugal rotor assembly (27) press against each other; Then, the fixative injection needle assembly (603) is controlled to be located directly above the sample centrifuge rotor assembly (27), and the fixative injection needle (6031) is inserted into the corresponding test tube (276) containing the counterweight or sample; Subsequently, the fixative solenoid valve (620) corresponding to the number of the test tube (276) containing the counterweight or sample is opened, and the mixer motor (6220) is operated according to the sample volume to complete the operation of adding the fixative to all the test tubes (276) containing the counterweight or sample on the single sample centrifuge rotor assembly (27); Subsequently, the corresponding fixing liquid solenoid valve (620) is closed, and the reagent vertical movement motor (6001) on the reagent vertical movement module (600) is reset; then, step i is performed to precipitate and shake evenly, completing the fixing operation of all the test tubes (276) with counterweights or samples placed on the single sample centrifuge rotor assembly (27); After the fixing operation is repeated for all the test tubes (276) with weights or samples placed on the sample centrifuge rotor assembly (27), the centrifugation in step f, the supernatant in step g, and the needle cleaning in step h are performed; after completing steps f, g, and h, the precipitation and shaking in step i are performed again as a whole, thereby completing the first fixing process; After the first fixation process is completed, there are second and third fixation processes; 1. Test tube removal step: After completing step k, all test tubes (276) containing weights or samples are removed after the processing; at this time, the chromosome samples obtained in the test tubes (276) containing samples can be used for the subsequent preparation of chromosome sample slides, so as to perform observation and karyotype analysis under a microscope; Bring the sample centrifugal rotor assembly (27) on the centrifugal disk (23) to the test tube placement position, and then prompt the user on the touch screen (101) to remove all the test tubes (276) with weights or samples placed on the current sample centrifugal rotor assembly (27). After the removal is completed, press the confirmation key on the touch screen (101); After the confirmation button is pressed, the control device (10) reads the diffuse reflection sensor group (30) to perform test tube detection. After confirming that the test tube (276) with the counterweight or sample placed on the current sample centrifugal rotor assembly (27) has been completely removed, the centrifugal servo motor (20) is driven to move, and the next group of sample centrifugal rotor assemblies (27) on the centrifugal disk (23) is brought to the test tube placement position, and the above test tube removal process is repeated until the counterweight or sample test tube (276) on all sample centrifugal rotor assemblies (27) are removed, completing the test tube removal step.

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