Aquatic feed compound enzyme growth promoter and preparation method thereof

Through the combination of lysozyme and preservatives, combined with the adhesion of pectin and oil, the use of plant carbon black to absorb ultraviolet rays has solved the shortcomings of aquatic feed composite enzyme growth promoters in bactericidal, antiseptic, floating and UV stability, and achieved more efficient fish nutrition absorption and growth promotion.

CN120477291APending Publication Date: 2025-08-15JIANGSU YOUHENG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510659197.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-21
Publication Date
2025-08-15

AI Technical Summary

Technical Problem

The existing aquatic feed composite enzyme growth promoters have shortcomings in killing bacteria in feed, reducing preservative damage to fish, floating properties, polymerization of proteases and feed, and preventing ultraviolet damage.

Method used

The combination of lysozyme and preservatives is used to combine the adhesion of pectin and oil, and the ultraviolet rays are absorbed by plant carbon black, combined with components such as vitamins, amylase, and cellulase to prepare aquatic feed complex enzyme growth promoter.

Benefits of technology

Effectively prevent mold, reduce the use of preservatives, improve floating performance, reduce ultraviolet damage, enhance protein decomposition efficiency, and promote fish growth.

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Abstract

The invention discloses an aquatic feed compound enzyme growth promoter and a preparation method thereof, and relates to the technical field of enzyme preparation growth promoters. The aquatic feed compound enzyme growth promoter comprises the following components in parts by weight: 8-16 parts of compound vitamins, 2-4 parts of magnesium acetate, 2-4 parts of betaine, 4-6 parts of antibacterial functional enzyme, 15-30 parts of pectin, 10-20 parts of plant carbon black, 10-20 parts of grease, 20 parts of compound lytic enzyme, 4-6 parts of amylase, 4-6 parts of traditional Chinese medicine powder, 4-6 parts of a preservative, 10-20 parts of cellulase and 2-4 parts of compound hormone. Mildew of the compound enzyme growth promoter can be effectively reduced by adding the lysozyme and the preservative, meanwhile, the content of the preservative can be reduced by adding the lysozyme, the amount of the preservative eaten by fishes is reduced, and therefore damage of the preservative to the fishes is reduced.
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Description

Technical Field

[0001] The invention relates to the technical field of enzyme preparation growth promoters, in particular to an aquatic feed composite enzyme growth promoter and a preparation method thereof. Background Art

[0002] Compound enzyme production promoter is a compound enzyme promoter that can effectively decompose feed or food in farmed organisms. It can decompose large molecular proteins, starch and cellulose in feed or food into small molecules, thereby improving the fish's absorption efficiency of nutrients in feed or other foods, thereby promoting fish growth. The combination of compound enzymes and other types of growth promoters such as traditional Chinese medicine and hormones can promote fish growth more comprehensively.

[0003] The defects of existing complex enzyme growth promoters are: 1. The prior art JPS63133952A discloses an animal growth promoter, which does not kill bacteria in feed. When preservatives are used to prevent feed spoilage, long-term consumption of large amounts of preservatives by fish can easily affect their health. Eating fish that have accumulated preservatives can also easily cause harm to the human body. Therefore, a composite enzyme growth promoter for aquatic feed that can kill bacteria in feed while reducing the damage of preservatives to fish is needed to solve this problem.

[0004] 2. The prior art JPH08333263A discloses an animal growth promoter, which does not float on the water surface. After the promoter is mixed with feed, the feed easily sinks to the bottom of the water, making it difficult for fish to use the feed and resulting in feed waste. Therefore, a composite enzyme growth promoter for aquatic feed that can float and reduce the amount of sinking to the bottom of the water is needed to solve this problem.

[0005] 3. The prior art US07608444B2 discloses the use of acid-stable proteases in animal feed. This technology does not polymerize the protease and feed. When the mixed feed is scattered in the water, the compound enzyme is easily decomposed by the fish activity, causing the feed to sink to the bottom of the water. Therefore, a compound enzyme growth promoter for aquatic feed that can polymerize the feed and the compound enzyme growth promoter is needed to solve this problem.

[0006] 4. Prior art CN103865900B discloses a feed compound enzyme containing alkaline xylanase and a preparation method thereof. This technology does not have the function of reducing the damage of ultraviolet rays to proteases. When the compound enzyme on the water surface or in the open air is irradiated by ultraviolet rays, the compound enzyme is easily destroyed by ultraviolet rays, causing the compound enzyme to lose the ability to decompose substances such as protein in the feed, thereby reducing the absorption and utilization rate of the feed by fish. Therefore, there is a need for an aquatic feed compound enzyme growth promoter that can prevent the protease in the compound enzyme growth promoter from being destroyed by ultraviolet rays to solve this problem. Summary of the Invention

[0007] One purpose of the present application is to provide an aquatic feed compound enzyme growth promoter and a preparation method thereof, which can solve the technical problems raised in the prior art.

[0008] To achieve the above object, the present invention provides the following technical solution: a composite enzyme growth promoter for aquatic feed, comprising, by weight, 8 to 16 parts of composite vitamins, 2 to 4 parts of magnesium acetate, 2 to 4 parts of betaine, 4 to 6 parts of antibacterial functional enzymes, 15 to 30 parts of pectin, 10 to 20 parts of plant carbon black, 10 to 20 parts of oil, 20 parts of composite decomposition enzymes, 4 to 6 parts of amylase, 4 to 6 parts of traditional Chinese medicine powder, 4 to 6 parts of preservatives, 10 to 20 parts of cellulase and 2 to 4 parts of composite hormones.

[0009] Preferably, the vitamin complex comprises 2 to 4 parts of vitamin C, 2 to 4 parts of vitamin D, and 2 to 4 parts of vitamin E by weight.

[0010] Preferably, the antibacterial functional enzyme is lysozyme.

[0011] Preferably, the oil is one or more of soybean oil, rapeseed oil, palm oil and linseed oil.

[0012] Preferably, the composite decomposition enzyme comprises 10 parts of subtilisin and 10 parts of aspergillus protease by weight.

[0013] Preferably, the Chinese medicinal powder is one or more of hawthorn, tangerine peel and angelica.

[0014] Preferably, the preservative is one of sodium dehydroacetate or potassium sorbate.

[0015] Preferably, the compound hormone is one or more of a steroid hormone and a thyroid hormone.

[0016] Preferably, the preparation method of the aquatic feed compound enzyme growth promoter comprises the following steps: S1. Place Bacillus subtilis powder in Petri dish A, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight, and let stand at 20° C. for 48 hours to obtain subtilisin; S2. Aspergillus niger powder was placed in a culture dish B. 15 g of soybean meal powder, 15 g of sodium chloride, 25 g of peptone, 10 g of starch, 25 g of agar, and 1200 mL of distilled water were added to the culture dish B by weight. The mixture was allowed to stand at 20° C. for 48 hours to obtain koji proteinase. S3. The products in the culture dish A in step S1 and the culture dish B in step S2 are moved to a stirring device, and then multivitamins, magnesium acetate, betaine, antibacterial functional enzymes, amylase, Chinese medicine powder, preservatives, cellulase and compound hormones are added to the stirring device respectively, and stirred for 20 minutes; S4. The stirring device is then placed inside a hot air drying device, with a drying temperature of 40 degrees Celsius and a drying time of 40 minutes.

[0017] Preferably, the step S4 further includes the following steps: S41. After drying in the stirring device for 40 minutes, pectin, plant carbon black and oil are added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The invention can effectively reduce the mildew of the complex enzyme growth promoter by adding lysozyme and preservatives. At the same time, the addition of lysozyme can reduce the content of preservatives, reduce the amount of preservatives consumed by fish, and thus reduce the damage of preservatives to fish.

[0019] When the complex enzyme growth promoter and fish feed are mixed and scattered on the water surface, the possibility of the complex enzyme growth promoter being decomposed in water can be reduced through the mixed bonding and gluing effect of pectin and oil with other materials in the complex enzyme growth promoter and the feed. Therefore, under the action of the oil, the complex enzyme growth promoter floats on the water surface for a long time and avoids falling to the bottom of the water, thereby making it easier for fish to eat the complex enzyme growth promoter and reducing the waste of the complex enzyme growth promoter.

[0020] The invention reduces the dispersion of the complex enzyme growth promoter in water caused by the activities of fish by using the certain polymerization effect of pectin, thereby ensuring the integrity of the complex enzyme growth promoter.

[0021] The invention can absorb ultraviolet rays through the plant carbon black, thereby reducing the damage of ultraviolet rays to lysozyme, amylase, cellulase, subtilisin and aspergillus protease, ensuring that various proteases effectively decompose protein, cellulose or starch in feed, and lysozyme kills bacteria in feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is a flow chart of the preparation method of the present invention; DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] See also Figure 1 A compound enzyme growth promoter for aquatic feed, which comprises, by weight, 8 to 16 parts of complex vitamins, 2 to 4 parts of magnesium acetate, 2 to 4 parts of betaine, 4 to 6 parts of antibacterial functional enzymes, 15 to 30 parts of pectin, 10 to 20 parts of plant carbon black, 10 to 20 parts of oil, 20 parts of compound decomposition enzymes, 4 to 6 parts of amylase, 4 to 6 parts of traditional Chinese medicine powder, 4 to 6 parts of preservatives, 10 to 20 parts of cellulase and 2 to 4 parts of compound hormones.

[0025] The complex vitamin comprises 2 to 4 parts of vitamin C, 2 to 4 parts of vitamin D, and 2 to 4 parts of vitamin E by weight.

[0026] The antibacterial functional enzyme is lysozyme.

[0027] The oil is one or more of soybean oil, rapeseed oil, palm oil and linseed oil.

[0028] The composite decomposition enzyme comprises 10 parts of subtilisin and 10 parts of aspergillus protease by weight.

[0029] The Chinese medicinal powder is one or more of hawthorn, tangerine peel and angelica.

[0030] The preservative is one of sodium dehydroacetate or potassium sorbate.

[0031] Compound hormones are one or more of steroid hormones and thyroid hormones.

[0032] The preparation method of the aquatic feed compound enzyme growth promoter comprises the following steps: S1. Place Bacillus subtilis powder in Petri dish A, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight, and let stand at 20° C. for 48 hours to obtain subtilisin; S2. Aspergillus niger powder was placed in a culture dish B. 15 g of soybean meal powder, 15 g of sodium chloride, 25 g of peptone, 10 g of starch, 25 g of agar, and 1200 mL of distilled water were added to the culture dish B by weight. The mixture was allowed to stand at 20° C. for 48 hours to obtain koji proteinase. S3. The products in the culture dish A in step S1 and the culture dish B in step S2 are moved to a stirring device, and then multivitamins, magnesium acetate, betaine, antibacterial functional enzymes, amylase, Chinese medicine powder, preservatives, cellulase and compound hormones are added to the stirring device respectively, and stirred for 20 minutes; S4. The stirring device is then placed inside a hot air drying device, with a drying temperature of 40 degrees Celsius and a drying time of 40 minutes.

[0033] S4 also includes the following steps: S21. After drying in the stirring device for 40 minutes, pectin, vegetable carbon black and oil are added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter.

[0034] Subtilisin and aspergillus protease can effectively decompose the protein in the feed, vitamin C, vitamin D and vitamin E can supplement the nutritional elements of fish and promote fish growth, magnesium acetate and oil can promote fish growth, betaine can attract fish to eat, lysozyme and preservatives can play a preservative role, so that the growth promoter and feed will not deteriorate for a long time, amylase can decompose the starch in the feed, cellulase can decompose the cellulose in the feed, compound hormones can promote rapid growth of fish, Chinese medicine powder can promote the metabolic growth of fish, and at the same time can attract fish to eat. Example 1

[0035] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 4 parts of lysozyme, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After the stirring device is dried for 40 minutes, 20 parts of pectin, 15 parts of vegetable carbon black and 20 parts of soybean oil are added to the stirring device, followed by mechanical stirring for 10 minutes to obtain an aquatic feed compound enzyme growth promoter. Example 2

[0036] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After the stirring device is dried for 40 minutes, 20 parts of pectin, 15 parts of vegetable carbon black and 20 parts of soybean oil are added to the stirring device, followed by mechanical stirring for 10 minutes to obtain an aquatic feed compound enzyme growth promoter. Example 3

[0037] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 4 parts of lysozyme, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After drying in the stirring device for 40 minutes, 15 parts of vegetable carbon black and 20 parts of soybean oil were added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter. Example 4

[0038] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 4 parts of lysozyme, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After the stirring device is dried for 40 minutes, 20 parts of pectin and 20 parts of soybean oil are added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter. Example 5

[0039] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 4 parts of lysozyme, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After the stirring device is dried for 40 minutes, 20 parts of pectin and 15 parts of vegetable carbon black are added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter. Example 6

[0040] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 4 parts of lysozyme, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 6 parts of potassium sorbate, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After drying in the stirring device for 40 minutes, 15 parts of plant carbon black were added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter. Example 7

[0041] 1. Place Bacillus subtilis powder in Petri dish A. Add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight. Let stand at 20°C for 48 hours to obtain subtilisin. 2. Place the Aspergillus niger powder into Petri dish B, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar and 1200 mL distilled water into Petri dish B according to weight, and let it stand at 20°C for 48 hours to obtain Aspergillus acid protease; 3. The products in the culture dish A in step 1 and the culture dish B in step S2 were moved to a stirring device, and then 4 parts of vitamin C, 4 parts of vitamin D, 4 parts of vitamin E, 4 parts of magnesium acetate, 4 parts of betaine, 6 parts of amylase, 1 part of hawthorn, 1 part of dried tangerine peel and 2 parts of angelica, 15 parts of cellulase and 4 parts of steroid hormone were added to the stirring device respectively, and stirred for 20 minutes; 4. Then place the stirring device inside the hot air drying device, the drying temperature is 40 degrees Celsius, and the drying time is 40 minutes; 5. After the stirring device is dried for 40 minutes, 20 parts of pectin, 15 parts of vegetable carbon black and 20 parts of soybean oil are added to the stirring device, followed by mechanical stirring for 10 minutes to obtain an aquatic feed compound enzyme growth promoter.

[0042] Performance testing: Mildew resistance test: The products of each embodiment were placed in 25°C water and observed for mildew after 72 hours. At the same time, the products of each embodiment were observed to see if they sank to the bottom of the water. Anti-ultraviolet test: The products of each example were irradiated with ultraviolet light for 12 hours, then crushed and placed in 25°C starch water. After 72 hours, the products were observed for mildew.

[0043] Test data of each embodiment under the same single variable test conditions

[0044] Experimental data show that the present invention can effectively reduce the mildew of the complex enzyme growth promoter by adding lysozyme and a preservative. At the same time, the addition of lysozyme can reduce the content of the preservative and the amount of preservative consumed by fish, thereby reducing the damage of the preservative to the fish. When the complex enzyme growth promoter and fish feed are mixed and spread on the water surface, the possibility of the complex enzyme growth promoter decomposing in water can be reduced by the mixed bonding and gluing effect of pectin and oil with other materials in the complex enzyme growth promoter and the feed. As a result, under the action of oil, the complex enzyme growth promoter floats on the water surface for a long time and avoids falling to the bottom of the water, thereby making it easier for fish to consume the complex enzyme growth promoter and reducing the waste of the complex enzyme growth promoter. In addition, pectin has a certain polymerization effect, which reduces the dispersion of the complex enzyme growth promoter in water caused by fish activities and ensures the integrity of the complex enzyme growth promoter. The plant carbon black can absorb ultraviolet rays, thereby reducing the damage of ultraviolet rays to lysozyme, amylase, cellulase, subtilisin and aspergillus protease.

[0045] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description. It is intended that all variations within the meaning and range of equivalents of the claims be embraced herein, and any reference signs in the claims should not be construed as limiting the rights involved.

Claims

1. A composite enzyme growth promoter for aquatic feed, characterized by: The aquatic feed compound enzyme growth promoter comprises, by weight, 8 to 16 parts of compound vitamins, 2 to 4 parts of magnesium acetate, 2 to 4 parts of betaine, 4 to 6 parts of antibacterial functional enzymes, 15 to 30 parts of pectin, 10 to 20 parts of plant carbon black, 10 to 20 parts of oil, 20 parts of compound decomposition enzymes, 4 to 6 parts of amylase, 4 to 6 parts of traditional Chinese medicine powder, 4 to 6 parts of preservatives, 10 to 20 parts of cellulase and 2 to 4 parts of compound hormones.

2. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The vitamin complex comprises 2 to 4 parts of vitamin C, 2 to 4 parts of vitamin D, and 2 to 4 parts of vitamin E by weight.

3. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The antibacterial functional enzyme is lysozyme.

4. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The oil is one or more of soybean oil, rapeseed oil, palm oil and linseed oil.

5. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The composite decomposition enzyme comprises 10 parts of subtilisin and 10 parts of aspergillus protease by weight.

6. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The Chinese medicinal powder is one or more of hawthorn, tangerine peel and angelica.

7. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The preservative is one of sodium dehydroacetate or potassium sorbate.

8. The aquatic feed compound enzyme growth promoter according to claim 1, characterized in that: The compound hormone is one or more of a steroid hormone and a thyroid hormone.

9. The method for preparing a composite enzyme growth promoter for aquatic feed according to any one of claims 1 to 8, characterized in that: The preparation method of the aquatic feed compound enzyme growth promoter comprises the following steps: S1. Place Bacillus subtilis powder in Petri dish A, add 15 g soybean meal powder, 15 g sodium chloride, 25 g peptone, 10 g starch, 25 g agar, and 1200 mL distilled water to Petri dish A by weight, and let stand at 20° C. for 48 hours to obtain subtilisin; S2. Aspergillus niger powder was placed in a culture dish B. 15 g of soybean meal powder, 15 g of sodium chloride, 25 g of peptone, 10 g of starch, 25 g of agar, and 1200 mL of distilled water were added to the culture dish B by weight. The mixture was allowed to stand at 20° C. for 48 hours to obtain koji proteinase. S3. The products in the culture dish A in step S1 and the culture dish B in step S2 are moved to a stirring device, and then multivitamins, magnesium acetate, betaine, antibacterial functional enzymes, amylase, Chinese medicine powder, preservatives, cellulase and compound hormones are added to the stirring device respectively, and stirred for 20 minutes; S4. The stirring device is then placed inside a hot air drying device, with a drying temperature of 40 degrees Celsius and a drying time of 40 minutes.

10. The method for preparing a composite enzyme growth promoter for aquatic feed according to claim 9, characterized in that: The step S4 also includes the following steps: S41. After drying in the stirring device for 40 minutes, pectin, plant carbon black and oil are added to the stirring device, followed by mechanical stirring for 10 minutes, thereby obtaining an aquatic feed compound enzyme growth promoter.

Citation Information

Patent Citations

  • A feed complex enzyme containing alkaline xylanase and its preparation method

    CN103865900B

  • Refillable polyester vessel and preform for forming said vessel

    JP1988000042A

  • Use of acid stable protease in animal feed

    US7608444B2