Composition for preventing and controlling pigeon epidemic diseases as well as preparation method and application of composition
By using compositions of Ligustrum, Morindae, Cistanche, Cistanche, Big Green Leaf and Rehmannia, the problem of preventing and controlling pigeon pox viral diseases is solved, efficient and simple epidemic prevention and control effects are achieved, and the immunity and anti-viral ability of pigeons are improved.
Patent Information
- Application Number
- CN202510628063.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-15
- Publication Date
- 2025-08-15
AI Technical Summary
The existing technology is difficult to effectively and easily prevent and control pigeon pox virus diseases, and vaccination consumes a lot of financial and manpower.
The compositions of Ligustrum, Morindaemon, Cistanche, Cistanche, and Rehmannia are used to improve pigeon immunity and prevent and control pigeon pox viral diseases after processing through a specific process.
It improves the immunity of pigeons, significantly improves the prevention and control effect of pigeon pox virus, and enhances the body's antiviral ability and immune function.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of drug preparation, and in particular relates to a composition for preventing and controlling pigeon diseases, a preparation method and an application thereof. Background Art
[0002] As a high-protein, low-fat food rich in various nutrients, pigeons are experiencing a continuous growth in market demand. Younger consumers, in particular, are more inclined to choose green, organic, and high-quality foods, creating a promising market opportunity for the pigeon breeding industry.
[0003] Pigeons are often susceptible to a wide variety of diseases during breeding, including viral, bacterial, and other diseases. Pigeon pox is a highly contagious viral disease caused by the pigeon pox virus, which primarily infects young pigeons. Following infection, the virus rapidly reproduces in the body, and symptoms begin to appear after an incubation period. Vaccination is the primary means of preventing and controlling pigeon pox, but this method requires significant financial, human, and material resources. Therefore, finding a quick, simple, and effective way to prevent and control pigeon pox is a challenge urgently needed by those skilled in the art. Summary of the Invention
[0004] The purpose of the present invention is to provide a composition for preventing and controlling pigeon diseases, a preparation method and an application thereof. The preparation process is simple, and the obtained composition can improve the immunity of pigeons and effectively prevent and control pigeon pox.
[0005] The invention provides a composition for preventing and controlling pigeon diseases, comprising the following components: Ligustrum lucidum fruit (Fructus Ligustri Lucidi), Morinda officinalis (Morinda officinalis), Cistanche deserticola (Cistanche deserticola), Isatis indigotica (Isatis indigotica), Radix Rehmanniae Preparata (Radix Rehmanniae Preparata) and Rosa laevigatae (Fructus Rosae Laevigatae).
[0006] Preferably, the following components are included in parts by mass: 28-32 parts of Ligustrum lucidum fruits, 16-20 parts of Morinda officinalis roots, 15-18 parts of Cistanche deserticola roots, 12-15 parts of Isatis indigotica leaves, 8-12 parts of Rehmannia root prepared and 5-6 parts of Rosa laevigata fruits.
[0007] The present invention provides a method for preparing the composition described in the above technical solution, comprising the following steps:
[0008] soaking the Ligustrum lucidum fruit in a bacterial solution of Aspergillus oryzae to obtain soaked Ligustrum lucidum fruit;
[0009] drying and crushing the soaked Ligustrum lucidum fruits in sequence to obtain crushed Ligustrum lucidum fruits;
[0010] Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and then pulverized to obtain a pulverized product;
[0011] Reflux extraction is performed on the crushed material using ethanol to obtain an extract;
[0012] The crushed isatis indigotica leaf, crushed glossy privet fruit and the extract are mixed, and then ultrasonically dispersed, freeze-dried and passed through a 100-mesh sieve in sequence, and the sieve residue obtained is the composition.
[0013] Preferably, when the Ligustrum lucidum fruit is soaked in Aspergillus oryzae liquid, the effective viable bacteria count of the Aspergillus oryzae liquid is 3×10 8 -3.5×10 8 cfu / mL;
[0014] The soaking parameters include: time of 3-5 hours and temperature of 28-32°C.
[0015] Preferably, the volume mass ratio of the ethanol to the crushed material is 7-8 mL: 1 g;
[0016] The volume concentration of the ethanol is 65%-75%.
[0017] Preferably, the parameters of the reflux extraction include: temperature of 80-85° C., time of 1.5-2.5 h, and number of times of 2-3 times.
[0018] Preferably, the ultrasonic dispersion parameters include: power of 300-400w, time of 35-45min, temperature of 50-55°C;
[0019] The freeze-drying parameters include: temperature of 3-5° C. and time of 8-10 min.
[0020] The present invention provides the use of the composition described in the above technical solution in the preparation of a product for preventing and controlling pigeon diseases.
[0021] Preferably, the types of diseases include: pigeon pox.
[0022] The present invention provides a medicine for preventing and controlling pigeon diseases, comprising: the composition described in the above technical solution.
[0023] Beneficial effects:
[0024] The present invention provides a composition for preventing and controlling pigeon diseases, comprising the following components: Ligustrum lucidum fruit, Morinda officinalis root, Cistanche deserticola root, Isatis indigotica leaf, Rehmannia root prepared, and Rosa laevigata fruit. The Ligustrum lucidum fruit provided by the present invention has antiviral activity, effectively inhibiting viral replication and proliferation, thereby helping pigeons resist viral infection; Morinda officinalis root and Cistanche deserticola root have anti-fatigue and immune-enhancing effects; Isatis indigotica leaf can alleviate heat and toxic symptoms in pigeons by clearing away heat and detoxifying; Rehmannia root prepared promotes normal growth and development of pigeons by nourishing yin and replenishing blood; and Rosa laevigata fruit has the effect of maintaining intestinal balance. Therefore, after the above ingredients are specifically combined, the ingredients can synergistically enhance their effectiveness, improve the immunity of pigeons, and prevent and control pigeon diseases.
[0025] Based on the above technical advantages, the present invention can effectively prevent and control pigeon diseases by using the composition described in the above technical solution to prepare a product for preventing and controlling pigeon diseases. DETAILED DESCRIPTION
[0026] In the present invention, unless otherwise specified, the materials, equipment and methods used are conventional ones.
[0027] There is no special requirement for the sources of the Morinda officinalis, Cistanche deserticola, Isatis indigotica, Rehmannia glutinosa, Rosa laevigata fruit and Ligustrum lucidum fruit, and they can be purchased conventionally; the Ligustrum lucidum fruit is preferably the fruit of Ligustrum lucidum fruit; the Rehmannia glutinosa is the processed root of Rehmannia glutinosa, a plant of the Scrophulariaceae family; there is no special requirement for the medicinal parts of the Morinda officinalis, Cistanche deserticola, Isatis indigotica and Rosa laevigata fruit, and conventionally used parts can be used.
[0028] In the present invention, when the Ligustrum lucidum fruit is soaked with the Aspergillus oryzae liquid, there is no special requirement for the amount of the Aspergillus oryzae liquid used, as long as the Ligustrum lucidum fruit is completely submerged in the liquid.
[0029] In order to further illustrate the present invention, the solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be understood as limiting the scope of protection of the present invention.
[0030] Example 1
[0031] Preparation of composition for preventing and controlling pigeon diseases
[0032] (1) Based on the number of preparations, the composition comprises: 28 parts of Ligustrum lucidum fruit, 16 parts of Morinda officinalis root, 18 parts of Cistanche deserticola root, 13 parts of Isatis indigotica leaf, 10 parts of Rehmannia root and 5 parts of Rosa laevigata fruit.
[0033] (2) Preparation method:
[0034] At 28-30℃, soak the fruit of Ligustrum lucidum in Aspergillus oryzae solution (the effective viable bacteria count of Aspergillus oryzae solution is 3×10 8 cfu / mL) for 3 h, and obtain the soaked Ligustrum lucidum fruit;
[0035] Drying the soaked Ligustrum lucidum fruits at 45° C. for 2 h, and crushing the dried Ligustrum lucidum fruits to obtain crushed Ligustrum lucidum fruits (at this point, the particle size of the Ligustrum lucidum fruits is ≤1 cm);
[0036] The cleaned and dried Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and crushed to obtain a crushed product (at this time, the particle size of the crushed product is ≤1 cm);
[0037] The crushed material was mixed with 65% ethanol at a mass volume ratio of 1 g:7 mL, and refluxed at 85° C. for 2.5 h, the reflux extraction was repeated twice, and an extract was obtained.
[0038] The cleaned and dried Folium Isatidis is ground into powder to obtain ground Folium Isatidis (at this point, the particle size of Folium Isatidis is ≤ 1 cm);
[0039] The crushed Folium Isatidis, the crushed Fructus Ligustri Lucidi and the extract are mixed, and ultrasonically dispersed at 350W and 52°C for 35 minutes to obtain a sonicated material; the sonicated material is freeze-dried at 5°C for 10 minutes to obtain a dried material; the dried material is crushed again and passed through a 100-mesh sieve, and the sieved material obtained is the composition.
[0040] Example 2
[0041] The only difference from Example 1 is step (1), which is as follows:
[0042] (1) Based on the number of preparations, the composition comprises: 30 parts of Ligustrum lucidum fruit, 20 parts of Morinda officinalis root, 15 parts of Cistanche deserticola root, 13 parts of Isatis indigotica leaf, 8 parts of Rehmannia root and 5 parts of Rosa laevigata fruit.
[0043] Example 3
[0044] The only difference from Example 1 is step (2), which is as follows:
[0045] (2) Preparation method:
[0046] At 28-30℃, soak the fruit of Ligustrum lucidum in Aspergillus oryzae solution (the effective viable bacteria count of Aspergillus oryzae solution is 3.5×10 8 cfu / mL) for 3 h, and obtain the soaked Ligustrum lucidum fruit;
[0047] Drying the soaked Ligustrum lucidum fruits at 45° C. for 2 h, and crushing the dried Ligustrum lucidum fruits to obtain crushed Ligustrum lucidum fruits (at this point, the particle size of the Ligustrum lucidum fruits is ≤1 cm);
[0048] The cleaned and dried Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and crushed to obtain a crushed product (at this time, the particle size of the crushed product is ≤1 cm);
[0049] The crushed material was mixed with 75% ethanol at a mass volume ratio of 1 g:7 mL, and refluxed at 85° C. for 2.5 h, with the reflux extraction performed twice, to obtain an extract;
[0050] The cleaned and dried Folium Isatidis is ground into powder to obtain ground Folium Isatidis (at this point, the particle size of Folium Isatidis is ≤ 1 cm);
[0051] The crushed Folium Isatidis, the crushed Fructus Ligustri Lucidi and the extract are mixed, and ultrasonically dispersed at 350W and 55°C for 35 minutes to obtain a sonicated material; the sonicated material is freeze-dried at 5°C for 10 minutes to obtain a dried material; the dried material is crushed again and passed through a 100-mesh sieve, and the sieved material obtained is the composition.
[0052] Comparative Example 1
[0053] The only difference from Example 1 is steps (1) and (2), which are as follows:
[0054] (1) Based on the number of preparations, the composition comprises: 28 parts of Ligustrum lucidum fruit, 16 parts of Morinda officinalis root, 18 parts of Cistanche deserticola root, 13 parts of Isatis indigotica leaf, 10 parts of Astragalus root and 5 parts of Rosa laevigata fruit.
[0055] And in the preparation method (2), Rehmannia root is replaced by Astragalus root.
[0056] Comparative Example 2
[0057] The only difference from Example 1 is step (2), which is as follows:
[0058] (2) Preparation method:
[0059] The cleaned Ligustrum lucidum fruit was dried at 45°C for 2 hours, and the dried Ligustrum lucidum fruit was crushed to obtain crushed Ligustrum lucidum fruit (at this time, the particle size of Ligustrum lucidum fruit was ≤1 cm);
[0060] The cleaned and dried Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and crushed to obtain a crushed product (at this time, the particle size of the crushed product is ≤1 cm);
[0061] The crushed material was mixed with 65% ethanol at a mass volume ratio of 1 g:7 mL, and refluxed at 85° C. for 2.5 h, the reflux extraction was repeated twice, and an extract was obtained.
[0062] The cleaned and dried Folium Isatidis is ground into powder to obtain ground Folium Isatidis (at this point, the particle size of Folium Isatidis is ≤ 1 cm);
[0063] The crushed Folium Isatidis, the crushed Fructus Ligustri Lucidi and the extract are mixed, and ultrasonically dispersed at 350W and 52°C for 35 minutes to obtain a sonicated material; the sonicated material is freeze-dried at 5°C for 10 minutes to obtain a dried material; the dried material is crushed again and passed through a 100-mesh sieve, and the sieved material obtained is the composition.
[0064] Comparative Example 3
[0065] The only difference from Example 1 is step (2), which is as follows:
[0066] (2) Preparation method:
[0067] At 28-30℃, soak the fruit of Ligustrum lucidum in Aspergillus oryzae solution (the effective viable bacteria count of Aspergillus oryzae solution is 3×10 8 cfu / mL) for 3 h, and obtain the soaked Ligustrum lucidum fruit;
[0068] Drying the soaked Ligustrum lucidum fruits at 45° C. for 2 h, and crushing the dried Ligustrum lucidum fruits to obtain crushed Ligustrum lucidum fruits (at this point, the particle size of the Ligustrum lucidum fruits is ≤1 cm);
[0069] The cleaned and dried Morinda officinalis, Cistanche deserticola, Rehmannia root, Rosa laevigata fruit and Folium Isatidis were mixed and pulverized to obtain a pulverized product (at this time, the particle size of the pulverized product was ≤1 cm);
[0070] The crushed material was mixed with 65% ethanol at a mass volume ratio of 1 g:7 mL, and refluxed at 85° C. for 2.5 h, the reflux extraction was repeated twice, and an extract was obtained.
[0071] The crushed Ligustrum lucidum fruit and the extract are mixed, and ultrasonically dispersed at 350w and 52°C for 35 minutes to obtain a sonicated material; the sonicated material is freeze-dried at 5°C for 10 minutes to obtain a dried material; the dried material is crushed again and passed through a 100-mesh sieve, and the material below the sieve is the composition.
[0072] Comparative Example 4
[0073] The only difference from Example 1 is step (2), which is as follows:
[0074] (2) Preparation method:
[0075] At 28-30℃, soak the fruit of Ligustrum lucidum in Aspergillus oryzae solution (the effective viable bacteria count of Aspergillus oryzae solution is 3×10 8 cfu / mL) for 3 h, and obtain the soaked Ligustrum lucidum fruit;
[0076] Drying the soaked Ligustrum lucidum fruits at 45° C. for 2 h, and crushing the dried Ligustrum lucidum fruits to obtain crushed Ligustrum lucidum fruits (at this point, the particle size of the Ligustrum lucidum fruits is ≤1 cm);
[0077] The cleaned and dried Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and crushed to obtain a crushed product (at this time, the particle size of the crushed product is ≤1 cm);
[0078] The crushed material was mixed with 65% ethanol at a mass volume ratio of 1 g:7 mL, and refluxed at 85° C. for 2.5 h, the reflux extraction was repeated twice, and an extract was obtained.
[0079] The cleaned and dried Folium Isatidis is ground into powder to obtain ground Folium Isatidis (at this point, the particle size of Folium Isatidis is ≤ 1 cm);
[0080] The ground Folium Isatidis, the ground Fructus Ligustri Lucidi and the extract are mixed, ground and passed through a 100-mesh sieve, and the sieve residue obtained is the composition.
[0081] Application Example 1
[0082] Eighty healthy, three-month-old white king pigeons were selected and artificially inoculated with pigeon pox virus (PPV) at five locations on the pigeons' hairless or sparsely haired areas, such as the corners of their eyes or under their abdomens. Pigeons that developed scabs at the inoculated areas served as experimental subjects. Pigeons with scabs were randomly divided into eight groups of ten pigeons each (i.e., a total of 50 scabs per treatment).
[0083] The compositions prepared in Examples 1-3 and Comparative Examples 1-4 were dissolved in water to obtain a test solution with a final concentration of 0.5 g / mL; the solution was fed to pigeons at a dose of 2 mL / kg body weight, once a day, for 10 consecutive days. The treatment of feeding the same mass of water was used as a blank control, and the descabbing and healing of pigeon pox in each treatment were observed, and the cure rate was statistically calculated. The results are shown in Table 1.
[0084] Table 1 Recovery status of pigeons in different treatments
[0085] deal with Number of pigeon pox scabs Number of pigeon pox healed Number of scabs Example 1 5 45 0 Example 2 6 44 0 Example 3 5 44 1 Comparative Example 1 10 35 5 Comparative Example 2 12 30 8 Comparative Example 3 11 36 3 Comparative Example 4 8 35 7 Blank control 4 0 46
[0086] The data in Table 1 show that after adopting the technical solutions in Examples 1-3, the scab removal and healing rates of pigeon pox were high, with the highest cure rate reaching 90%; while after adopting the technical solutions in Comparative Examples 1-4, the number of pigeon pox healed decreased sharply, with the highest healing rate being only 36. This shows that the changes in the raw materials, dosage or preparation method of the composition in the comparative examples were not conducive to the prevention and control of pigeon pox.
[0087] Application Example 2
[0088] Description of the breeding environment for experimental squabs: The squabs were raised in a glass greenhouse with a humidity of 60%-65%. The temperature requirements were as follows: the temperature was maintained at 38-39°C from 1 to 7 days old, and at 37-38°C from 8 to 14 days old. The temperature was then lowered by 1°C every day. After 20 days, artificial temperature control was stopped and the temperature of the greenhouse was maintained by natural light.
[0089] Based on the results of Application Example 1, the compositions prepared in Examples 1-3, Comparative Example 1, and Comparative Example 3 were selected for verification of Application Example 2: 120 healthy one-day-old squabs were selected and randomly divided into 6 groups, 20 in each group; the composition prepared in each treatment was fed to the pigeons at a dosage of 3.5% of the pigeons' body weight, and a treatment without the composition was used as a blank control; before feeding the pigeons on the 1st and 30th day, 3 squabs were randomly selected from each treatment and weighed. After being sacrificed by bleeding from the back of the neck, the spleen and thymus were removed, and the fresh weight of the squabs after fat removal was weighed. The average immune organ index was calculated for each pigeon. The results are shown in Table 2.
[0090] Among them, immune organ index (mg / g) = immune organ fresh weight (mg) / fasting live weight before slaughter (g).
[0091] Table 2 Average immune organ index of squabs in different treatments
[0092]
[0093] The weight of the thymus and spleen can be used to assess the immune status of the chicks. The larger the corresponding organ index, the stronger the body's cellular and humoral immune functions. Combining the data in Table 2, it can be seen that when the compositions prepared in Examples 1-3 were fed to squabs for 30 days, the thymus immune index of the squabs reached a maximum of 7.5241 mg / g, and the spleen immune index reached a maximum of 1.2415 mg / g; both were higher than the corresponding immune organ indices in the comparative example and the blank control. This shows that the compositions prepared by the present invention are beneficial for improving the cellular immunity of pigeons.
[0094] On the 28th day, before feeding the pigeons, three pigeons were randomly selected from each treatment for serum IgA, IgG, and IgM assays: blood was collected from the pigeons at the time of lethal treatment, and the serum was separated and frozen for preservation. The concentrations of each antibody were determined using ELISA test kits produced by GBD Company according to the requirements of the corresponding instructions. The results are shown in Table 3.
[0095] Table 3 Average antibody concentrations in squabs under different treatments
[0096]
[0097]
[0098] It can be seen from the data in Table 3 that, compared with the blank control and the comparative example, after the composition prepared in Examples 1-3 was fed to squab, the antibody concentrations of IgA, IgG and IgM in the serum of squab were significantly increased. It can be seen that the technical solution of the present invention is conducive to improving the immunity of pigeons, and is thus conducive to preventing and controlling the occurrence of pigeon diseases.
[0099] In summary, the Fructus Ligustri Lucidi provided by the present invention has antiviral activity, effectively inhibits viral replication and proliferation, thereby helping pigeons resist viral infection; Morinda officinalis has the effect of resisting fatigue and enhancing the body's immune function; Cistanche deserticola can increase the level of specific antibodies; Folium Isatidis can alleviate the symptoms of heat and toxicity in pigeons by clearing away heat and detoxifying; Radix Rehmanniae Preparata promotes the normal growth and development of pigeons by nourishing yin and replenishing blood; Fructus Rosae Laevigatae has the effect of maintaining intestinal balance. Therefore, after the above raw materials are specifically combined, each raw material can synergize and enhance the immunity of pigeons, and prevent and control the epidemic diseases of pigeons.
[0100] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.
Claims
1. A composition for preventing and controlling pigeon diseases, characterized in that: The invention comprises the following components: Fructus Ligustri Lucidi, Morinda Officinalis, Cistanche Deserticola, Folium Isatidis, Rehmanniae Preparata and Fructus Rosae Laevigatae.
2. The composition according to claim 1, characterized in that The invention comprises the following components in parts by mass: 28-32 parts of Ligustrum lucidum fruits, 16-20 parts of Morinda officinalis roots, 15-18 parts of Cistanche deserticola roots, 12-15 parts of Isatis indigotica leaves, 8-12 parts of Rehmannia root prepared and 5-6 parts of Rosa laevigata fruits.
3. The method for preparing the composition according to claim 1 or 2, characterized in that: The steps include: soaking the Ligustrum lucidum fruits in Aspergillus oryzae liquid to obtain soaked Ligustrum lucidum fruits; drying and crushing the soaked Ligustrum lucidum fruits in sequence to obtain crushed Ligustrum lucidum fruits; Morinda officinalis, Cistanche deserticola, Rehmannia root and Rosa laevigata fruit are mixed and then pulverized to obtain a pulverized product; Reflux extraction is performed on the crushed material using ethanol to obtain an extract; The crushed isatis indigotica leaf, crushed glossy privet fruit and the extract are mixed, and then ultrasonically dispersed, freeze-dried and passed through a 100-mesh sieve in sequence, and the sieve residue obtained is the composition.
4. The preparation method according to claim 3, characterized in that When the glossy privet fruit is soaked in the Aspergillus oryzae solution, the effective viable bacteria count of the Aspergillus oryzae solution is 3×10 8 -3.5×10 8 cfu / mL; The soaking parameters include: time of 3-5 hours and temperature of 28-32°C.
5. The preparation method according to claim 3, characterized in that The volume mass ratio of the ethanol to the crushed material is 7-8 mL:1 g; The volume concentration of the ethanol is 65%-75%.
6. The preparation method according to claim 3, characterized in that The parameters of the reflux extraction include: temperature of 80-85° C., time of 1.5-2.5 h, and number of times of 2-3 times.
7. The preparation method according to claim 3, characterized in that The parameters of the ultrasonic dispersion include: power of 300-400w, time of 35-45min, temperature of 50-55°C; The freeze-drying parameters include: temperature of 3-5° C. and time of 8-10 min.
8. Use of the composition according to claim 1 or 2 in preparing a product for preventing and controlling pigeon diseases.
9. The use according to claim 8, characterized in that The types of diseases include: pigeon pox.
10. A drug for preventing and controlling pigeon diseases, characterized in that: The medicine comprises: the composition according to claim 1 or 2.