Bacterial liquid preparation device in anaerobic environment

Abstract

The invention relates to the technical field of flora transplantation treatment, in particular to bacterial liquid preparation equipment in an anaerobic environment, which comprises an anaerobic glove box, a buffer partition box, an operation power assisting device, a glove body, an airtight delivery window device and a pickup device, an isolation window is arranged on the front side of the anaerobic glove box, the glove body is embedded on the isolation window, and the airtight delivery window device is arranged on the isolation window. The glove body comprises a sealing ring body used for sealing the butt joint isolation window, an operation display screen is arranged on the upper portion of the anaerobic glove box, an operation power assisting device is installed at the top end of the interior of the anaerobic glove box and comprises an auxiliary micro camera, and the operation display screen can be used for observing pictures shot by the auxiliary micro camera. A flora extraction device and centrifugal separation equipment are arranged at the bottom end in the anaerobic glove box. According to the bacteria liquid preparation equipment in the anaerobic environment, the strict anaerobic environment is provided, and the survival rate of anaerobic flora in the transplanting process is remarkably increased through the sealed steady-state environment.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial colony transplantation and processing, and in particular to a bacterial liquid preparation device under an anaerobic environment. Background Art

[0002] With the advancement of medical technology, researchers have discovered that human health is closely linked to the structure of our microbiome. The intestinal flora not only participates in the digestion and absorption of nutrients but also plays a key role in immune regulation, metabolic balance, and neural signaling. An imbalance in the intestinal microbiome can lead to a variety of diseases.

[0003] Intestinal microbial transplantation, an emerging therapeutic approach, extracts and prepares the intestinal flora of a healthy donor and transplants it into the patient's digestive tract, restoring the microecological balance and thereby treating the disease. This treatment method primarily works by interacting with the patient's intestinal tissue cells to exert its therapeutic effect. However, over 90% of the microbial communities in the human gut are strictly anaerobic, rapidly inactivating or even dying in the presence of oxygen. Therefore, maintaining a stable, anaerobic environment during the preparation of the microbial culture is crucial. The routine process for intestinal microbial transplantation includes donor screening, sample collection, microbial culture preparation, quality testing, and clinical transplantation. The microbial culture preparation step is particularly critical, as it involves homogenizing, filtering, and centrifuging the fecal sample, and preparing the microbial suspension. The entire process requires strict oxygen avoidance and is handled in an anaerobic environment to ensure the activity of these microorganisms.

[0004] Existing anaerobic sample processing equipment lacks adequate sealing performance and effective anaerobic environment control methods, resulting in low survival rates of anaerobic bacteria during transplantation, which in turn impacts transplantation effectiveness and directly affects the stability and repeatability of clinical efficacy. Therefore, there is an urgent need for a bacterial solution preparation device with a well-sealed anaerobic environment to effectively improve the success rate of anaerobic transplantation. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a bacterial liquid preparation device under anaerobic environment in view of the deficiencies of the existing technology.

[0006] The technical problem to be solved by the present invention is achieved through the following technical solutions. The present invention discloses a bacterial liquid preparation device under an anaerobic environment, including an anaerobic glove box, a buffer compartment box, an operation assist device, a glove body, an airtight transfer window device and a picking device. The front side of the anaerobic glove box is provided with an isolation window, the isolation window is embedded with a glove body, the glove body includes a sealing ring body for sealing the isolation window, the upper part of the anaerobic glove box is provided with an operation display screen, the inner top of the anaerobic glove box is equipped with an operation assist device, the operation assist device includes an auxiliary micro camera, and the operation display screen can be used to observe the auxiliary micro camera. The picture taken by the micro-camera shows that a bacterial colony extraction device and a centrifugal separation device are provided at the bottom inner end of the anaerobic glove box, and both the bacterial colony extraction device and the centrifugal separation device include a sliding mechanism. The buffer compartment box is adjacently arranged on the side of the anaerobic glove box, and an airtight transfer window device for dynamic isolation is provided between the anaerobic glove box and the buffer compartment box. Both the anaerobic glove box and the buffer compartment box are provided with an air pressure display device and a nitrogen and oxygen detection device. The anaerobic glove box and the buffer compartment box are respectively connected to a first air extraction and replacement system and a second air extraction and replacement system. A buffer window and a sealing window are provided at the front of the buffer compartment box, and the sealing window includes a sealing lock pin assembly.

[0007] The technical problem to be solved by the present invention can also be further achieved through the following technical solutions: the operating assist device includes a top hanging platform and a sliding joint, the top hanging platform is provided with a hanging plate, the hanging plate is provided with a slide groove, the sliding joint is slidably connected to the slide groove, and the lower part of the sliding joint is provided with a hand-held pendulum column.

[0008] The technical problem to be solved by the present invention can be further achieved by the following technical solution: the lower part of the sliding joint is connected to a hand-held pendulum column through a universal head, and the hand-held pendulum column is installed with an auxiliary micro camera through an elastic stretching kit.

[0009] The technical problem to be solved by the present invention can also be further achieved through the following technical solutions: a temperature control system and a humidity control system are provided inside the anaerobic glove box; the first air extraction and replacement system and the second air extraction and replacement system both include an air extraction device for replacing air and nitrogen; the first air extraction and replacement system is configured to trigger a quick-start working mode, and the second air extraction and replacement system is configured to intermittently start and adjust the working mode.

[0010] The technical problem to be solved by the present invention can also be further achieved through the following technical solutions. The airtight transfer window device includes a partition body, a central drive shaft and a rotary cover. The partition body is arranged between the anaerobic glove box and the buffer compartment box. The partition body is provided with an installation opening. The central drive shaft rotates vertically on the installation opening. The central drive shaft is connected to a driving device. A rotary window shaft is fixedly mounted on the outer side of the central drive shaft. A rotary cover is slidably mounted on the outer side of the rotary window shaft.

[0011] The technical problem to be solved by the present invention can also be further achieved through the following technical solution: a sealing block is provided at the edge of the partition body, and a rubber strip group is provided at the front of the sealing block for contacting and sealing with the outer cylindrical surface of the rotating window shaft. The rubber strip group includes vertical rubber strips arranged at intervals, and the outer contour line of the overall vertical projection of the rubber strip group is umbrella-shaped.

[0012] The technical problem to be solved by the present invention can be further achieved by the following technical solution: a receiving slot is provided on the side of the rotating window shaft, the cross section of the receiving slot is a quarter circle, and the receiving slot is provided with an adsorption seat for receiving the bacterial container.

[0013] The technical problem to be solved by the present invention can also be further achieved through the following technical solution: the cross-section of the rotary cover is a three-quarter ring, a rack is attached to the outside of the rotary cover, the partition body is provided with a stepping drive device, the stepping drive device is provided with a toothed rubber wheel, and the rotary cover is driven and connected via the toothed rubber wheel.

[0014] The technical problem to be solved by the present invention can also be further achieved through the following technical solution. The anaerobic glove box is provided with a picking device near the airtight transfer window device. The picking device includes a parallel four-bar swing mechanism and a horizontal docking plate. A reset airbag package is provided in the middle of the parallel four-bar swing mechanism. The front and rear ends of the horizontal docking plate are respectively provided with an electromagnetic attraction device and a handle. The picking device is used to assist in picking up samples transferred by the airtight transfer window device.

[0015] Compared with the prior art, the present invention has the following advantages:

[0016] (1) The bacterial liquid preparation device under anaerobic environment provided by the present invention can provide a strict anaerobic environment and significantly improve the survival rate of anaerobic bacteria during the transplantation process by sealing the steady-state environment.

[0017] (2) The present invention uses a sealed glove box in conjunction with a buffer compartment box to achieve overall sealing, thereby reducing the risk of contamination caused by the migration of bacteria in a dynamic operating environment. At the same time, through efficient disinfection and isolation measures, cross contamination and other problems are avoided.

[0018] (3) The design of the anaerobic glove box of the present invention allows the operator to operate without directly contacting the sample, which is easy to operate. In addition, the high dynamic sealing structure can be used for other experimental operations of bacterial liquids that require strict anaerobic conditions in addition to enterobacteria transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the main view of the overall structure of the present invention;

[0020] Figure 22 is a bottom view of the structure of the operating assist device 3 of the present invention;

[0021] Figure 3 Schematic diagram of the structure of the airtight transfer window device 5 of the present invention;

[0022] Figure 4 It is a schematic structural diagram of the pickup device 6 of the present invention.

[0023] 1-Anaerobic glove box, 11-Isolation window, 12-Operation display screen, 13-Second air extraction and replacement system, 14-Bacteria extraction device, 15-Centrifugal separation device, 16-Sliding mechanism, 2-Buffer compartment, 21-Buffer window, 22-Sealing window, 221-Sealing lock pin assembly, 23-First air extraction and replacement system, 3-Operation power assist device, 4-Glove body, 41-Sealing ring body, 5-Airtight transfer window device, 51-Partition body, 511-sealing block, 52-center drive shaft, 521-rotating window shaft, 522-holding slot, 522a-adsorption seat, 53-rotating cover, 531-stepping drive device, 532-toothed rubber wheel, 6-picking device, 61-parallel four-bar swing mechanism, 61a-resetting airbag package, 611-horizontal docking plate, 611a-handle, 611b-electromagnetic attraction device, 7-air pressure display device, 8-nitrogen and oxygen detection device. DETAILED DESCRIPTION

[0024] like Figure 1-4 As shown, a bacterial liquid preparation device under an anaerobic environment of the present invention includes an anaerobic glove box 1, a buffer compartment box 2, an operation assist device, a glove body 4, an airtight transfer window device 5 and a picking device 6.

[0025] An isolation window 11 is provided on the front side of the anaerobic glove box 1, and a glove body 4 is embedded in the isolation window 11. The glove body 4 includes a sealing ring body 41 for sealing and docking the isolation window 11. An operation display screen 12 is provided on the upper part of the anaerobic glove box 1. An operation power assist device is installed on the top of the inner top of the anaerobic glove box 1. The operation power assist device includes an auxiliary micro camera. The operation display screen 12 can be used to observe the shooting picture of the auxiliary micro camera.

[0026] Specifically, the operating assist device 3 includes a top platform and a sliding joint. The top platform is equipped with a hanging plate, which is equipped with a slide slot. The sliding joint is slidably connected to the slide slot. The lower portion of the sliding joint is connected to a hand-held pendulum via a universal joint. The hand-held pendulum is equipped with an auxiliary micro-camera via an elastic stretch kit. Because the hands are constantly inside the box and cantilevered through gloves, fatigue can easily occur. Therefore, the provision of a power assist structure not only reduces fatigue but also helps the operator operate more accurately.

[0027] A bacterial flora extraction device 14 and a centrifugal separation device 15 are provided at the bottom of the anaerobic glove box 1 . The centrifugal separation device 15 includes a centrifuge device and a separator device. Both the bacterial flora extraction device 14 and the centrifugal separation device 15 include a sliding mechanism 16 .

[0028] The buffer compartment box 2 is adjacent to the side of the anaerobic glove box 1. An airtight transfer window device 5 for dynamic isolation is provided between the anaerobic glove box 1 and the buffer compartment box 2. A picking device 6 is provided near the airtight transfer window device 5 of the anaerobic glove box 1. The picking device 6 assists in hand operation to avoid excessive movement.

[0029] The structure of the airtight transfer window device 5 is further described below. It includes a partition body 51, a central drive shaft 52 and a rotary cover 53. The partition body 51 is arranged between the anaerobic glove box 1 and the buffer compartment box 2. The partition body 51 is provided with a mounting opening. A sealing block 511 is provided at the edge of the partition body 51. The front of the sealing block 511 is provided with a rubber strip group for contacting and sealing with the outer cylindrical surface of the rotary window shaft 521. The rubber strip group includes vertical rubber strips arranged at intervals, and the outer contour line of the overall vertical projection of the rubber strip group is umbrella-shaped.

[0030] Among them, the central driving shaft 52 rotates vertically on the mounting port, the central driving shaft 52 is connected to a driving device, a rotating window shaft 521 is fixedly sleeved on the outside of the central driving shaft 52, and a rotating cover 53 is slidingly sleeved on the outside of the rotating window shaft 521. A holding slot 522 is opened on the side of the rotating window shaft 521, and the cross-section of the holding slot 522 is a quarter circle. The holding slot 522 is provided with an adsorption seat 522a for holding a bacterial container.

[0031] The cross section of the rotary cover 53 is three-quarters ring-shaped. A rack is attached to the outside of the rotary cover 53. The partition body 51 is provided with a stepping drive device 531. The stepping drive device 531 is provided with a toothed rubber wheel 532. The rotary cover 53 is driven and connected via the toothed rubber wheel 532.

[0032] According to the above structural configuration, the corresponding specific movement steps are as follows:

[0033] The notch of the rotary cover 53 coincides with the receiving slot 522 of the rotary window shaft 521 at the initial position, thereby opening the space of the receiving slot 522. After the bacterial sample container is placed from the outside, the air is replaced with nitrogen, and then the rotary cover 53 is rotated 90° clockwise to lock the receiving slot 522. Then, the rotary window shaft 521 and the rotary cover 53 are synchronously rotated 90° clockwise, and the bacterial sample enters the internal space of the anaerobic glove box 1. Then the rotary cover 53 is rotated 90° counterclockwise to realize the cover opening action. With the assistance of the picking device 6, the operator can smoothly and slowly remove the bacterial sample and place it in the corresponding processing device.

[0034] During the above process, two independent rotary drive mechanisms cooperate with a certain action rhythm to ensure that the sealing block 511 always maintains a closed contact with the whole formed by the rotary window shaft 521 and the rotary cover 53, thereby achieving a dynamic sealing effect, reducing the impact of the bacterial delivery process on the internal box gas environment, and at the same time reducing bacterial contamination and leakage.

[0035] Furthermore, the picking device 6 includes a parallelogram swing mechanism 61 and a horizontal docking plate 611. A reset airbag body 61a is provided in the middle of the parallelogram swing mechanism 61. An electromagnetic attraction device 611b and a handle 611a are provided at the front and rear ends of the horizontal docking plate 611, respectively. The picking device 6 is used to assist in picking up samples transferred from the airtight transfer window device 5. The samples transferred from the airtight transfer window device 5 are attracted to the horizontal docking plate 611 or the electromagnetic attraction device 611b by the electromagnetic attraction device 611b. The parallelogram swing mechanism 61 can swing left and right, synchronously driving the horizontal docking plate 611 and the sample above it to move left and right, thereby receiving the sample transferred from the airtight transfer window device 5 and moving it to a position close to the operator's hand, assisting the operator in picking up the sample, avoiding excessive movement amplitude, and reducing hand fatigue.

[0036] The anaerobic glove box 1 is provided with a temperature control system and a humidity control system. The first air extraction and replacement system 23 and the second air extraction and replacement system 13 both include an air extraction device for replacing air with nitrogen.

[0037] The anaerobic glove box 1 and the buffer compartment box 2 are both provided with an air pressure display device 7 and a nitrogen oxide detection device 8. The anaerobic glove box 1 and the buffer compartment box 2 are respectively connected to a first air extraction and replacement system 23 and a second air extraction and replacement system 13. A buffer window 21 and a sealing window 22 are provided at the front of the buffer compartment box 2. The sealing window 22 includes a sealing lock pin assembly 221.

[0038] Specifically, the first air extraction and replacement system 23 is configured to trigger and start a quick-opening working mode, and the second air extraction and replacement system 13 is configured to start an intermittent adjustment working mode.

[0039] The specific method is:

[0040] A second exhaust replacement system 13 is provided on the left side of the anaerobic glove box 1, which is used to realize the nitrogen concentration replacement process under the control of the sensor control system. The main working state is normally closed and is only activated when the concentration exceeds the threshold value to intermittently maintain a constant concentration.

[0041] A first air extraction and replacement system 23 is installed on the right side of the buffer compartment 2. After the bacterial colony container is placed and the sealing window 22 is closed, the first air extraction and replacement system 23 is used to extract and replace the oxygen in the air within the buffer compartment 2 with nitrogen, until an anaerobic environment is achieved within the anaerobic glove box 1. Furthermore, when the first and second air extraction and replacement systems 23 and 13 are in operation, they can quickly establish or maintain a positive pressure state within the corresponding enclosed space, forming a holistic, dynamically and statically sealed operating space in conjunction with the airtight transfer window assembly 5.

[0042] The apparatus of the present invention fully considers the issue of operating environment contamination, namely, preventing the ingress of outside air. Therefore, the aforementioned pumping and displacement action can achieve a positive pressure process in the internal gas environment, or alternatively, can ensure a continuous steady state, i.e., maintain the internal gas environment within a strictly proportional state, as required. The present invention can achieve a steady state process by equipping the apparatus with an internal gas environment detection device and a gaseous environment stabilization device.

[0043] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A bacterial liquid preparation device under anaerobic environment, characterized by: The anaerobic glove box comprises an anaerobic glove box, a buffer compartment, an operation assist device, a glove body, an airtight transfer window device and a picking device. The front side of the anaerobic glove box is provided with an isolation window, the glove body is embedded in the isolation window, the glove body includes a sealing ring body for sealing and docking the isolation window, the upper part of the anaerobic glove box is provided with an operation display screen, the inner top end of the anaerobic glove box is installed with an operation assist device, the operation assist device includes an auxiliary micro camera, and the operation display screen can be used to observe the shooting image of the auxiliary micro camera. The inner bottom end of the anaerobic glove box is provided with a bacterial flora extraction device and a centrifugal separation device, both of which include a sliding mechanism. The buffer compartment is adjacent to the side of the anaerobic glove box, and an airtight transfer window device for dynamic isolation is provided between the anaerobic glove box and the buffer compartment. The anaerobic glove box and the buffer compartment are both provided with an air pressure display device and a nitrogen and oxygen detection device. The anaerobic glove box and the buffer compartment are respectively connected to a first air extraction and replacement system and a second air extraction and replacement system. The front part of the buffer compartment is provided with a buffer window and a sealing window, and the sealing window includes a sealing lock pin assembly.

2. The bacterial liquid preparation device under anaerobic environment according to claim 1, characterized in that: The operation assist device includes a top hanging platform and a sliding joint. The top hanging platform is provided with a hanging plate. The hanging plate is provided with a slide groove. The sliding joint is slidably connected to the slide groove. The lower part of the sliding joint is provided with a hand-held pendulum column.

3. The bacterial liquid preparation device under anaerobic environment according to claim 2, characterized in that: The lower part of the sliding joint is connected to a hand-held pendulum column through a universal head, and the hand-held pendulum column is installed with an auxiliary micro camera through an elastic stretching kit.

4. The bacterial liquid preparation device under anaerobic environment according to claim 1, characterized in that: The anaerobic glove box is provided with a temperature control system and a humidity control system. The first air extraction and replacement system and the second air extraction and replacement system both include an air extraction device for replacing air with nitrogen. The first air extraction and replacement system is configured to trigger a quick-open working mode, and the second air extraction and replacement system is configured to intermittently start and adjust the working mode.

5. The bacterial liquid preparation device under anaerobic environment according to claim 1, characterized in that: The airtight transfer window device includes a partition body, a central drive shaft and a rotary cover. The partition body is arranged between the anaerobic glove box and the buffer compartment box. The partition body is provided with a mounting opening. The central drive shaft rotates vertically on the mounting opening. The central drive shaft is connected to a driving device. A rotary window shaft is fixedly mounted on the outer side of the central drive shaft. A rotary cover is slidably mounted on the outer side of the rotary window shaft.

6. The bacterial liquid preparation device under anaerobic environment according to claim 5, characterized in that: A sealing block is provided at the edge of the partition body, and a rubber strip group is provided in front of the sealing block for contacting and sealing with the outer cylindrical surface of the rotating window shaft. The rubber strip group includes vertical rubber strips arranged at intervals, and the outer contour line of the overall vertical projection of the rubber strip group is umbrella-shaped.

7. The bacterial liquid preparation device under anaerobic environment according to claim 6, characterized in that: A holding notch is provided on the side of the rotary window shaft, the cross section of the holding notch is a quarter circle, and the holding notch is provided with an adsorption seat for holding a bacterial colony container.

8. The bacterial liquid preparation device under anaerobic environment according to claim 5, characterized in that: The cross section of the rotary cover is three-quarters ring-shaped, a rack is attached to the outside of the rotary cover, the partition body is provided with a stepping drive device, the stepping drive device is provided with a toothed rubber wheel, and the rotary cover is driven and connected via the toothed rubber wheel.

9. The bacterial liquid preparation device under anaerobic environment according to claim 1, characterized in that: The anaerobic glove box is provided with a picking device near the airtight transfer window device, which includes a parallel four-bar swing mechanism and a horizontal docking plate. A reset airbag package is provided in the middle of the parallel four-bar swing mechanism, and an electromagnetic attraction device and a handle are provided at the front and rear ends of the horizontal docking plate respectively. The picking device is used to assist in picking up samples transferred from the airtight transfer window device.

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