Temperature control type tumor cell culture device

Abstract

The invention discloses a temperature control type tumor cell culture device, and relates to the technical field of medical instruments. The liquid adding device comprises a box body, and two liquid adding assemblies are arranged in the box body. When liquid is added into the culture dish, along with evaporation of a culture solution in the culture dish, the liquid level in the culture dish is gradually lowered, the culture solution in the liquid adding box is conveyed into the culture dish through the valve, the evaporated culture solution in the culture dish is supplemented, and after supplementing, the liquid level in the liquid adding box is gradually lowered; through the arrangement of the assembly, the problem that the nutrient solution cannot be supplemented in time due to evaporation loss is effectively solved, the effect of automatically supplementing the nutrient solution can be achieved, meanwhile, the problem that workers need to monitor in real time is solved, and the working efficiency is improved. The survival rate of tumor cells is improved, the problem that nutrient substances in the culture solution sink to the bottom is also avoided, and the effect of stirring the culture solution can be achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a temperature-controlled tumor cell culture device. Background Art

[0002] Temperature-controlled tumor cell culture devices are a type of experimental equipment that has been widely used in the field of tumor research in recent years. They are designed to provide a stable, controllable environment for tumor cell culture, simulating the in vivo tumor microenvironment, and thus providing reliable data support for research on tumor biology, drug screening, and anti-tumor therapy. Traditional cell culture devices mostly rely on conventional incubators, but these devices have the problem of difficult to precisely control temperature and gas concentration. Temperature-controlled tumor cell culture devices have been optimized based on this, and can provide more precise temperature control. They can not only regulate the temperature within the incubator, but also maintain constant gas in the microenvironment, ensuring optimal cell growth conditions. In addition, with the continuous in-depth study of tumor biology, increasing evidence shows that tumor cell growth is not only affected by temperature changes, but also regulated by multiple factors such as nutrition, oxygen, and pH. By providing tumor cells with a precisely controllable culture environment, temperature-controlled tumor cell culture devices have greatly promoted tumor cell research, especially in drug development and personalized research on tumor treatment.

[0003] A document (publication number: CN114672416B) discloses a tumor cell culture device. Each mating tooth plate engages with a corresponding mating tooth, enabling each rotating mounting rod to rotate during movement, thereby causing each vessel placement plate to rotate accordingly. This allows the assembly to adjust the angle of each culture dish while performing multiple movements, ensuring that every surface of the culture dish is heated. This effectively addresses the problem of uneven internal heating, which can lead to lower temperatures in some culture dishes and prevent successful cell culture. However, during actual use, the culture medium in the culture dish gradually evaporates into water vapor due to temperatures exceeding room temperature, resulting in a gradual decrease in the amount of culture medium in the dish. This makes it impossible to ensure the survival of tumor cells, reduces the success rate of tumor cell culture, and consumes a lot of experimenter's energy. Furthermore, harmful substances produced by the tumor cells are deposited in large quantities in the culture dish, affecting the survival rate of the tumor cells, making the device unable to ensure the successful survival of tumor cells during use. Summary of the Invention

[0004] The purpose of the present invention is to solve the above problems and provide a temperature-controlled tumor cell culture device.

[0005] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions: A temperature-controlled tumor cell culture device comprises a housing, wherein two sets of liquid-adding assemblies are disposed within the housing, each set of the liquid-adding assemblies comprising a liquid-adding box, a first adjusting rod, a second adjusting rod, a floating plate, a second screw, a first screw, a stirring blade, a sealing plug, and a second spring, wherein the first adjusting rod is fixedly connected to the sealing plug, one end of the second spring is fixedly connected to the first adjusting rod, the other end of the second spring is fixedly connected to the second adjusting rod, the second adjusting rod is fixedly connected to the floating plate, the floating plate is fixedly connected to the second screw, the second screw is threadedly connected to the first screw, and the first screw is fixedly connected to the stirring blade; A culture dish is fixedly installed inside the box, and a drainage assembly is provided inside the culture dish. The drainage assembly includes an adjustment plate, a spring, a sealing pad, a pressure rod, a receiving plate, a fixing block, a sealing plate, a bottom plate and a fixing ring.

[0006] Furthermore, the adjustment plate is fitted with the culture dish, the adjustment plate is fixedly connected to the sealing gasket, the sealing gasket is slidably connected to the culture dish, one end of the spring one is fixedly connected to the sealing gasket, the other end of the spring one is fixedly connected to the bottom plate, the bottom plate is fixedly connected to the culture dish, the pressure rod is fitted with the surface of the adjustment plate, the receiving plate is fixedly connected to the pressure rod, the receiving plate is fixedly connected to the fixed block, the fixed block is fixedly connected to the sealing plate, the sealing plate is fitted with the inner wall of the liquid adding box, the fixing ring is fixedly connected to the sealing plate, and the fixing ring is fixedly connected to the adjustment rod two.

[0007] Furthermore, two groups of drainage grooves are provided inside the bottom plate, and drainage holes are provided on the inner walls of the two groups of drainage grooves. A drainage pipe is fixedly installed inside the box body, and the drainage pipe is communicated with the drainage holes.

[0008] Furthermore, a truncated table is fixedly installed inside the culture dish, a partition net is fixedly installed on the upper surface of the truncated table, and the partition net and the culture dish are located at the same horizontal plane.

[0009] Furthermore, valves are fixedly installed on both sides of the culture dish, the valves are fixedly connected to the liquid adding box, and a slope block is fixedly installed inside the liquid adding box, and the slope block and the valve are located on the same horizontal plane.

[0010] Furthermore, a second heating tube is fixedly installed inside the liquid adding box, and a first heating tube is fixedly installed inside the liquid adding box.

[0011] Furthermore, a partition is fixedly connected to the interior of the liquid adding box, the partition is fitted with a sealing plug, observation tubes are fixedly installed on both sides of the box body, and a liquid adding tube is fixedly installed on the upper surface of the box body, and the liquid adding tube is connected to the liquid adding box.

[0012] Furthermore, a universal wheel is fixedly mounted on the lower surface of the box, and a foot brake is fixedly mounted on the surface of the universal wheel.

[0013] Furthermore, a box door is fixedly mounted on the surface of the box body, a handle is fixedly mounted on the surface of the box door, exhaust holes are opened on both sides of the box body, and a display screen is fixedly mounted on the surface of the box body.

[0014] Furthermore, two groups of fixing rods are fixedly installed inside the liquid adding box, and one end of the two groups of fixing rods is fixedly connected to a limiting tube, the limiting tube is fitted with the surface of the adjusting rod 2, and the limiting tube is fitted with the surface of the adjusting rod 1.

[0015] The beneficial effects of the present invention are as follows: 1. When the culture dish is added with liquid, as the culture liquid inside the culture dish evaporates, the liquid level inside the culture dish gradually decreases. The culture liquid inside the liquid adding box is transferred to the culture dish through the valve to replenish the culture liquid evaporated from the culture dish. After replenishment, the liquid level inside the liquid adding box gradually decreases, causing the float plate, the second regulating rod and the second screw to gradually decrease. The second screw drives the first screw and the stirring blade to rotate, thereby stirring the nutrients deposited at the bottom of the liquid adding box, thereby preventing the nutrients in the culture liquid from sinking to the bottom. As the second regulating rod descends, the compressed second spring gradually returns to its original position. When the elastic force of the second spring is less than the weight of the sealing plug and the first regulating rod, the sealing plug is closed. The plug is separated from the partition, allowing the culture fluid above the partition to flow into the bottom of the partition to replenish the culture fluid below. The inflow of culture fluid causes the liquid level to gradually rise, causing the float plate to gradually return to its original position, causing spring 2 to be compressed again, and then causing the sealing plug to re-fit with the partition, thereby stopping the inflow of culture fluid above the partition. The setting of this component effectively avoids the problem of evaporation and loss of culture fluid and the inability to replenish it in time, and can achieve the effect of automatically replenishing the culture fluid. At the same time, it avoids the problem of staff needing real-time monitoring, improves the survival rate of tumor cells, and avoids the problem of nutrients in the culture fluid sinking to the bottom, and can achieve the effect of stirring the culture fluid.

[0016] 2. The present invention replenishes the culture fluid inside the culture dish through a valve. The impact force of the replenished culture fluid causes the harmful substances produced on the upper surface of the circular table to fall onto the surface of the adjusting plate. As the liquid level gradually drops through the float plate and the adjusting rod 2, the fixed ring, the sealing plate, the fixed block and the receiving plate follow the adjusting rod 2 to move vertically downward, so that the pressure rod drives the adjusting plate and the sealing gasket to move vertically downward, so that the spring 1 is compressed, so that the culture fluid at the bottom of the culture dish and the harmful substances produced by the tumor cells flow into the drainage hole through the drainage groove and are discharged to the outside of the device through the drainage pipe. The setting of this component effectively avoids the problem of harmful substance deposition affecting the survival of tumor cells, and can achieve the effect of timely discharge of harmful substances. At the same time, it avoids the process of manual processing and improves the survival rate of tumor cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This invention Figure 1 Right view schematic diagram of the overall structure; Figure 3 This invention Figure 1 Look directly at the internal structure diagram; Figure 4 This invention Figure 3 Left view internal structure diagram; Figure 5 This invention Figure 4 A schematic diagram of the local structure from above; Figure 6 This invention Figure 4 Schematic diagram of the right view part structure; Figure 7 This invention Figure 6 Schematic diagram of the structure from above; Figure 8 This invention Figure 7 Looking up at the internal structure diagram; Figure 9 This invention Figure 7 Side view cross-sectional structure diagram.

[0018] Reference numerals: 1, box body; 11, universal wheel; 12, foot brake; 13, handle; 14, box door; 15, exhaust hole; 16, display screen; 17, liquid filling pipe; 18, observation pipe; 19, heating pipe 1; 110, heating pipe 2; 2, drainage assembly; 21, drainage pipe; 22, spring 1; 23, sealing pad; 24, pressure rod; 25, receiving plate; 26, fixing block; 27, sealing plate; 28, fixing ring; 29, adjustment plate; 210. Drain trough; 211. Drain hole; 3. Liquid adding assembly; 31. Liquid adding box; 32. Partition; 33. Adjustment rod 1; 34. Fixed rod; 35. Limiting tube; 36. Adjustment rod 2; 37. Screw 1; 38. Float plate; 39. Screw 2; 310. Stirring blade; 311. Sealing plug; 312. Spring 2; 313. Inclined block; 4. Culture dish; 41. Bottom plate; 42. Round table; 43. Partition net; 44. Valve. DETAILED DESCRIPTION

[0019] To make the objectives, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0020] Example 1, as Figures 1-9As shown, a temperature-controlled tumor cell culture device includes a housing 1, wherein two liquid adding assemblies 3 are disposed inside the housing 1. Each of the two liquid adding assemblies 3 includes a liquid adding box 31, an adjusting rod 1 33, an adjusting rod 2 36, a floating plate 38, a screw 2 39, a screw 1 37, a stirring blade 310, a sealing plug 311, and a spring 2 312. The adjusting rod 1 33 is fixedly connected to the sealing plug 311, one end of the spring 2 312 is fixedly connected to the adjusting rod 1 33, and the other end of the spring 2 312 is fixedly connected to the adjusting rod 2 36. The adjusting rod 2 36 is fixedly connected to the floating plate 38, the floating plate 38 is fixedly connected to the screw 2 39, the screw 2 39 is threadedly connected to the screw 1 37, and the screw 1 37 is fixedly connected to the stirring blade 310. A culture dish 4 is fixedly installed inside the box 1, and a drainage component 2 is provided inside the culture dish 4. The drainage component 2 includes an adjustment plate 29, a spring 22, a sealing gasket 23, a pressure rod 24, a receiving plate 25, a fixing block 26, a sealing plate 27, a bottom plate 41 and a fixing ring 28.

[0021] When culturing tumor cells using the temperature-controlled tumor cell culture device, it is necessary to fill the culture fluid in advance and heat the culture fluid to a suitable temperature. Then, the tumor cells are moved to the upper surface of the truncated table 42. When adding culture fluid to the device, the culture fluid is added to the liquid adding tank 31 through the liquid adding tube 17. At this time, there is no culture fluid under the partition 32. The floating plate 38 is located at the lowest end due to gravity, so that the screw 39 is in contact with the stirring blade 310. The culture fluid on the upper surface of the partition 32 gradually flows into the lower part of the partition 32 due to gravity. As the culture fluid flows in, the liquid The surface gradually rises, and the floating plate 38 floats up due to gravity. The floating plate 38 floats up, and the regulating rod 2 36 gradually rises due to the floating plate 38. When the liquid level is higher than the upper surface of the inclined surface block 313, the culture solution flows into the interior of the culture dish 4 through the valve 44. When the liquid level inside the culture dish 4 is flush with the liquid level inside the liquid adding tank 31, the buoyancy of the floating plate 38 makes the sealing plug 311 fit with the partition 32, so that the culture solution above the partition 32 cannot flow into the bottom of the partition 32. As the culture solution inside the culture dish 4 evaporates, the liquid level inside the culture dish 4 gradually drops, and the culture dish 4 needs to be adjusted. 4 is used to add liquid to prevent tumor cell death. The culture liquid in the liquid adding tank 31 is transferred to the culture dish 4 through the valve 44 to replenish the culture liquid evaporated from the culture dish 4. After replenishment, the liquid level in the liquid adding tank 31 gradually drops, causing the float plate 38, the second regulating rod 36 and the second screw 39 to gradually drop. The second screw 39 drives the first screw 37 and the stirring blade 310 to rotate, thereby stirring the nutrients deposited at the bottom of the liquid adding tank 31, thereby preventing the nutrients in the culture liquid from sinking to the bottom. As the regulating rod 36 drops, the compressed second spring 312 gradually Gradually return to its original position. When the elastic force of spring 2 312 is less than the gravity of sealing plug 311 and adjusting rod 1 33, sealing plug 311 separates from partition 32, allowing the culture fluid above partition 32 to flow into the bottom of partition 32 to replenish the culture fluid below. The inflow of culture fluid causes the liquid level to gradually rise, causing the float plate 38 to gradually return to its original position, causing spring 2 312 to be in a compressed state again, and then causing sealing plug 311 to fit into partition 32 again, thereby stopping the inflow of culture fluid above partition 32, and completing the replenishment of culture fluid in culture dish 4.

[0022] Example 2, as Figure 6-Figure 9As shown, on the basis of the above embodiment, it also includes: the adjustment plate 29 is fitted with the culture dish 4, the adjustment plate 29 is fixedly connected to the sealing gasket 23, the sealing gasket 23 is slidably connected to the culture dish 4, one end of the spring 22 is fixedly connected to the sealing gasket 23, the other end of the spring 22 is fixedly connected to the bottom plate 41, the bottom plate 41 is fixedly connected to the culture dish 4, the pressure rod 24 is fitted with the surface of the adjustment plate 29, the receiving plate 25 is fixedly connected to the pressure rod 24, the receiving plate 25 is fixedly connected to the fixing block 26, the fixing block 26 is fixedly connected to the sealing plate 27, the sealing plate 27 is fitted with the inner wall of the liquid adding box 31, the fixing ring 28 is fixedly connected to the sealing plate 27, the fixing ring 28 is fixedly connected to the adjustment rod 2 36, two groups of drainage grooves 210 are provided inside the bottom plate 41, and the inner walls of the two groups of drainage grooves 210 are provided with drainage holes 211. The interior of the box body 1 is fixed A drainage pipe 21 is fixedly installed, and the drainage pipe 21 is connected to the drainage hole 211. When the culture fluid in the culture dish 4 is replenished, the culture fluid inside the culture dish 4 is replenished through the valve 44. The impact force of the replenished culture fluid causes the harmful substances produced on the upper surface of the truncated table 42 to fall into the surface of the adjustment plate 29. After replenishment, the liquid level at the inclined block 313 drops. As the liquid level gradually drops through the floating plate 38 and the adjusting rod 2 36, the fixed ring 28, the sealing plate 27, the fixed block 26 and the receiving plate 25 follow the adjusting rod 2 36 to move vertically downward, so that the pressure rod 24 drives the adjusting plate 29 and the sealing gasket 23 to move vertically downward, so that the spring 1 22 is compressed, so that the culture fluid at the bottom of the culture dish 4 and the harmful substances produced by the tumor cells flow into the drainage hole 211 through the drainage groove 210 and are discharged to the outside of the device through the drainage pipe 21.

[0023] Example 3, as Figure 7 As shown, on the basis of the above embodiment, it also includes that a truncated cone 42 is fixedly installed inside the culture dish 4, and a partition net 43 is fixedly installed on the upper surface of the truncated cone 42. The partition net 43 and the culture dish 4 are located at the same horizontal plane. The partition net 43 fixedly installed on the upper surface of the truncated cone 42 can prevent tumor cells from falling into the upper surface of the adjustment plate 29 when harmful substances are conducted, thereby achieving the effect of protecting tumor cells.

[0024] Example 4, as Figure 5-Figure 7As shown, on the basis of the above embodiment, it also includes that valves 44 are fixedly installed on both sides of the culture dish 4, the valves 44 are fixedly connected to the liquid adding box 31, the interior of the liquid adding box 31 is fixedly installed with an inclined block 313, the inclined block 313 and the valve 44 are located at the same horizontal plane, the interior of the liquid adding box 31 is fixedly installed with a heating pipe 2 110, and the interior of the liquid adding box 31 is fixedly installed with a heating pipe 19. The culture fluid at the inclined block 313 can be transferred to the culture dish 4 through the valve 44 to replenish the evaporated culture fluid of the culture dish 4 to avoid the death of tumor cells. The culture fluid above the partition 32 is heated by the heating pipe 2 110, and the culture fluid below the partition 32 is heated by the heating pipe 19, thereby avoiding the death of tumor cells caused by the culture fluid temperature being too low.

[0025] Example 5, as Figures 1-4 As shown, on the basis of the above embodiment, it also includes that the interior of the liquid adding box 31 is fixedly connected to the partition 32, the partition 32 is fitted with the sealing plug 311, observation tubes 18 are fixedly installed on both sides of the box body 1, and a liquid adding tube 17 is fixedly installed on the upper surface of the box body 1, and the liquid adding tube 17 is connected to the liquid adding box 31. The observation tube 18 is convenient for the staff to observe the remaining culture medium content above the partition 32. When it is about to be used up, the culture medium is conveniently added through the liquid adding tube 17.

[0026] Example 6: Figure 1 As shown, on the basis of the above embodiment, it also includes that a universal wheel 11 is fixedly installed on the lower surface of the box 1, a foot brake 12 is fixedly installed on the surface of the universal wheel 11, a box door 14 is fixedly installed on the surface of the box door 14, a handle 13 is fixedly installed on the surface of the box door 14, exhaust holes 15 are provided on both sides of the box 1, and a display screen 16 is fixedly installed on the surface of the box 1. The universal wheel 11 and the foot brake 12 are provided to facilitate the movement of the device, and the handle 13 and the box door 14 are used to facilitate the staff to observe and take tumor cells. The temperature and humidity inside the box 1 can be displayed through the display screen 16.

[0027] Example 7, as Figure 4 As shown, on the basis of the above embodiment, it also includes that two groups of fixing rods 34 are fixedly installed inside the liquid filling box 31, and one end of the two groups of fixing rods 34 is fixedly connected to the limiting tube 35, the limiting tube 35 is in contact with the surface of the adjusting rod 2 36, and the limiting tube 35 is in contact with the surface of the adjusting rod 1 33. The limiting tube 35 is used to limit the adjusting rod 1 33 and the adjusting rod 2 36, so that the adjusting rod 1 33 and the adjusting rod 2 36 are more stable when moving vertically.

[0028] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A temperature-controlled tumor cell culture device, comprising a box (1), characterized in that: Two groups of liquid adding components (3) are provided inside the box body (1), and the two groups of liquid adding components (3) each include a liquid adding box (31), an adjusting rod (33), an adjusting rod (36), a floating plate (38), a screw (39), a screw (37), a stirring blade (310), a sealing plug (311) and a spring (312). The adjusting rod (33) is fixedly connected to the sealing plug (311), one end of the spring (312) is fixedly connected to the adjusting rod (33), the other end of the spring (312) is fixedly connected to the adjusting rod (36), the adjusting rod (36) is fixedly connected to the floating plate (38), the floating plate (38) is fixedly connected to the screw (39), the screw (39) is threadedly connected to the screw (37), and the screw (37) is fixedly connected to the stirring blade (310); A culture dish (4) is fixedly installed inside the box (1), and a drainage assembly (2) is provided inside the culture dish (4). The drainage assembly (2) includes an adjustment plate (29), a spring (22), a sealing gasket (23), a pressure rod (24), a receiving plate (25), a fixing block (26), a sealing plate (27), a bottom plate (41) and a fixing ring (28).

2. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: The adjusting plate (29) is fitted with the culture dish (4), the adjusting plate (29) is fixedly connected to the sealing gasket (23), the sealing gasket (23) is slidingly connected to the culture dish (4), one end of the spring (22) is fixedly connected to the sealing gasket (23), the other end of the spring (22) is fixedly connected to the bottom plate (41), the bottom plate (41) is fixedly connected to the culture dish (4), the pressure rod (24) is fitted with the surface of the adjusting plate (29), the receiving plate (25) is fixedly connected to the pressure rod (24), the receiving plate (25) is fixedly connected to the fixed block (26), the fixed block (26) is fixedly connected to the sealing plate (27), the sealing plate (27) is fitted with the inner wall of the liquid adding box (31), the fixing ring (28) is fixedly connected to the sealing plate (27), and the fixing ring (28) is fixedly connected to the adjusting rod (36).

3. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: Two groups of drainage grooves (210) are provided inside the bottom plate (41), and drainage holes (211) are provided on the inner walls of the two groups of drainage grooves (210). A drainage pipe (21) is fixedly installed inside the box body (1), and the drainage pipe (21) is connected to the drainage hole (211).

4. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: A truncated platform (42) is fixedly mounted inside the culture dish (4), and a partition net (43) is fixedly mounted on the upper surface of the truncated platform (42), wherein the partition net (43) and the culture dish (4) are located at the same horizontal plane.

5. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: Valves (44) are fixedly installed on both sides of the culture dish (4), and the valves (44) are fixedly connected to the liquid adding box (31). A slope block (313) is fixedly installed inside the liquid adding box (31), and the slope block (313) and the valve (44) are located on the same horizontal plane.

6. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: A second heating pipe (110) is fixedly installed inside the liquid adding box (31), and a first heating pipe (19) is fixedly installed inside the liquid adding box (31).

7. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: A partition (32) is fixedly connected to the interior of the liquid adding box (31), and the partition (32) is in contact with the sealing plug (311). Observation tubes (18) are fixedly installed on both sides of the box body (1). A liquid adding tube (17) is fixedly installed on the upper surface of the box body (1), and the liquid adding tube (17) is in communication with the liquid adding box (31).

8. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: A universal wheel (11) is fixedly mounted on the lower surface of the box body (1), and a foot brake (12) is fixedly mounted on the surface of the universal wheel (11).

9. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: A door (14) is fixedly mounted on the surface of the box body (1), a handle (13) is fixedly mounted on the surface of the door (14), exhaust holes (15) are provided on both sides of the box body (1), and a display screen (16) is fixedly mounted on the surface of the box body (1).

10. The temperature-controlled tumor cell culture device according to claim 1, characterized in that: Two groups of fixing rods (34) are fixedly installed inside the liquid adding box (31), and one end of each of the two groups of fixing rods (34) is fixedly connected to a limiting tube (35), and the limiting tube (35) is in contact with the surface of the second regulating rod (36), and the limiting tube (35) is in contact with the surface of the first regulating rod (33).

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