Method for preparing marine bioactive substance with repairing effect by utilizing marine product processing byproducts
By acid treatment and filtration of oyster shells and mussel cooking liquid, marine biological actives were prepared, which solved the problem of unefficient use of seafood by-products, and achieved the dual effects of skin repair and environmental gain.
Patent Information
- Application Number
- CN202510931467.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-07
- Publication Date
- 2025-08-19
AI Technical Summary
By-products generated during seafood processing such as oyster shells and mussel cooking liquid are not efficiently utilized, resulting in environmental pollution and waste of resources, and the existing technology has failed to effectively increase its added value.
By acid treatment of oyster shells and mussel cooking liquid, mixing and filtering, marine biological actives with skin repair effects, including compounding of calcium ions and polyols, forming marine biological actives.
It significantly promotes the increase of hyaluronic acid and cell migration of keratinocytes, has good skin repair effects, improves the added value of seafood by-products, and reduces environmental pollution.
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Figure CN120501686A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of high-value utilization of seafood by-products, and in particular to a method for preparing marine bioactive substances with repair efficacy by utilizing seafood processing by-products. Background Art
[0002] The processing of fish, crustaceans, and shellfish produces a large number of byproducts, such as fish bones, shrimp and crab shells, and shells. Most of these byproducts are directly discarded or processed into low-value fish meal, which can easily cause environmental pollution and waste resources. For example, the production and processing of oysters is mostly limited to the edible meat, while the shells, which account for over 60% of the oyster's mass, are not efficiently utilized. Research shows that for every kilogram of Pacific oysters consumed, approximately 370 to 700 grams of oyster shells are produced. In my country, approximately 10 million tons of shell waste must be processed annually. These discarded oyster shells are often discarded in landfills, which not only consumes valuable land and tidal flat resources, but also oxidizes and decays the organic matter in the discarded shells in the air, breeding a large number of pathogenic microorganisms, causing serious environmental pollution and waste of solid waste resources. Mussels, along with oysters and scallops, are considered one of the world's three major shellfish species. Mussels have a high harvest season with a short, high yield and are prone to spoilage if not processed promptly after harvesting. To extend the storage life of mussels, harvested mussels are typically steamed and stored as dried or frozen products. This process produces a large amount of mussel cooking liquid. It's reported that for every ton of mussels processed, 1.5 tons of cooking liquid is produced. Directly discharging this cooking liquid not only pollutes the environment but also wastes nutrients. Therefore, there's a need to strengthen the comprehensive utilization of these processing byproducts.
[0003] Based on this, if these discarded oyster shells and mussel cooking liquid waste can be recycled, it will not only bring considerable economic benefits, but also generate environmental gains, thereby achieving the purpose of environmental protection and value-added of marine organisms and realizing the sustainable and healthy development of the marine organism breeding and processing industry. Summary of the Invention
[0004] In response to the shortcomings of the existing technology, the present invention utilizes seafood processing by-products such as oyster shells and mussel cooking liquid to prepare a marine bioactive substance with skin repair effect, which not only increases the added value of seafood but also reduces environmental pollution.
[0005] In order to achieve the above purpose, the present invention solves the technical problem by adopting the following technical solutions:
[0006] In a first aspect, the present invention provides a method for preparing marine bioactive substances with repair efficacy using seafood processing byproducts, the method comprising the following steps:
[0007] S1. Take oyster shell powder and perform a first acid treatment to obtain a slurry;
[0008] S2. Take the mussel cooking liquid for a second acid treatment to obtain a slurry II;
[0009] S3. The slurry 1 and slurry 2 were mixed and reacted at 45 to 55 ° C for 80 to 100 min to obtain slurry 3;
[0010] S4. The slurry is centrifuged and filtered to obtain the filtrate, which is filtered through a 50 nm ceramic membrane and a 1000 Da ultrafiltration membrane in sequence. The filtrate is compounded with glycerol, polyols and preservatives to produce marine bioactive substances.
[0011] The present invention performs acid treatment on oyster shell powder and mussel cooking liquid, which can not only remove the fishy smell of the oyster shell and mussel cooking liquid, but also promote the dissolution of calcium, thereby increasing the calcium content in the active substance, so that the prepared marine biological active substance can significantly promote the increase of hyaluronic acid in keratinocytes and cell migration.
[0012] In some embodiments, in step S1, the first acid treatment is to add oyster shell powder to an acid solution and water, and react at a constant temperature of 35°C for 80 minutes to obtain slurry 1; the oyster shell powder is oyster shells crushed and passed through a 100-mesh sieve.
[0013] In some embodiments, in step S1, the liquid-solid ratio of the acid solution to the oyster shell powder is 6 mL / g or 10 mL / g; the liquid-solid ratio of the water to the oyster shell powder is 50 mL / g.
[0014] In some embodiments, in step S2, the second acid treatment is to take mussel cooking liquid and acid solution, react at a constant temperature of 80°C for 30 minutes to obtain slurry 2; the volume ratio of the mussel cooking liquid to the acid solution is 20:1 or 100:1.
[0015] In some embodiments, in step S3, the ratio of the slurry 2 to the slurry 1 is 30:1 or 45:1.
[0016] In some embodiments, the first acid treatment and the second acid treatment use a hydrochloric acid solution;
[0017] Optionally, in the turbid liquid system treated with the first acid and the second acid, H + The concentration is 3 mol / L.
[0018] In a second aspect, the present invention provides marine bioactive substances prepared by the above-mentioned method for preparing marine bioactive substances with repair efficacy using seafood processing by-products.
[0019] In a third aspect, the present invention also provides the use of the above-mentioned marine bioactive substances in cosmetic products.
[0020] In some embodiments, the cosmetic product is in the form of an aqueous solution, an emulsion, a cream, an oil, a spray, a powder or a gel.
[0021] Compared with the prior art, the present invention has the following beneficial effects:
[0022] The present invention provides a marine bioactive substance with skin repair efficacy prepared using seafood processing by-products such as oyster shells and mussel cooking liquid. The marine bioactive substance can significantly promote the increase of hyaluronic acid in keratinocytes and cell migration, and has a good skin repair effect.
[0023] The method of the present invention is simple to operate and suitable for large-scale industrial production. The preparation of natural active substances from seafood processing by-products is safe and non-toxic, thereby increasing the added value of seafood processing by-products and reducing environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the implementation. Obviously, the drawings described below are only some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0025] Figure 1 These are actual pictures of cell scratch experiments with different active substances in the examples of the present invention, in which: A is the blank group at 0 h; B is the blank group at 23 h; C is Example 1; D is Example 2; E is Example 3; and F is Example 4.
[0026] Figure 2 These are actual pictures of cell scratch experiments with different active ingredients in the comparative examples of the present invention. In the pictures: A is comparative example 1; B is comparative example 2; C is comparative example 3; D is comparative example 4; and E is comparative example 8. DETAILED DESCRIPTION
[0027] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0028] In the present invention, when referring to numerical ranges, unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as every value between such minimum and maximum values. Furthermore, when a range refers to an integer, every integer between the minimum and maximum values of the range is included. In addition, when multiple ranges are provided to describe a feature or characteristic, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges subsumed therein.
[0029] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.
[0030] In the present invention, the test methods used are conventional methods unless otherwise specified; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified.
[0031] Oyster shell origin: Beihai, Guangxi;
[0032] Mussel cooking liquid: A certain aquatic products limited liability company in Shengsi County, Zhoushan City.
[0033] Example 1
[0034] A method for preparing marine bioactive substances with repairing effects by utilizing seafood processing byproducts comprises the following steps:
[0035] 1. Crush the oyster shells into small pieces and grind them in a high-speed grinder. Pass the crushed shell powder through a 100-mesh sieve to obtain oyster shell powder.
[0036] 2. Take oyster shell powder, add 3 mol / L hydrochloric acid solution and an appropriate amount of purified water, and react at a constant temperature of 35°C for 80 minutes to obtain slurry 1; the liquid-solid ratio of the 3 mol / L hydrochloric acid solution to the oyster shell powder is 6 mL / g, and the liquid-solid ratio of the purified water to the oyster shell powder is 50 mL / g.
[0037] 3. Take mussel cooking liquid and 3 mol / L hydrochloric acid solution, react at a constant temperature of 80°C for 30 minutes to obtain slurry 2; the ratio of the mussel cooking liquid to the hydrochloric acid solution is 20:1.
[0038] 4. Slurry 1 and slurry 2 were mixed and the temperature was adjusted to 50°C, and the mixture was reacted for 90 minutes to obtain slurry 3; the ratio of slurry 2 to slurry 1 was 30:1.
[0039] 5. The slurry was centrifuged at 3000 rpm for 10 min and filtered to remove insoluble matter. The filtrate was then filtered using a 50 nm ceramic membrane. The retentate was then ultrafiltered using a 1000 Da ultrafiltration membrane to obtain a filtrate, which was then compounded with glycerol, polyols, and preservatives to produce marine bioactive substances.
[0040] The marine biological active substance comprises, by mass fraction, 25% of filtrate, 20% of glycerol, 0.7% of 1,2-hexanediol, 0.3% of preservative (p-hydroxyacetophenone), and the balance is purified water.
[0041] Example 2
[0042] The difference between Example 2 and Example 1 is that the liquid-to-solid ratio of the 3 mol / L hydrochloric acid solution to the oyster shell powder in step 2 is 10 mL / g, and the others are the same.
[0043] Example 3
[0044] The difference between Example 3 and Example 1 is that in step 3, the ratio of mussel cooking liquid to hydrochloric acid solution is 100:1, and the other steps are the same.
[0045] Example 4
[0046] The difference between Example 4 and Example 1 is that the ratio of slurry 2 to slurry 1 in step 3 is 45:1, and the other aspects are the same.
[0047] Comparative Example 1
[0048] The difference between Comparative Example 1 and Example 1 is that calcium chloride is used instead of oyster shells to prepare slurry 1 in Comparative Example 1. The preparation method of marine bioactive substances is as follows:
[0049] 1. Take mussel cooking liquid and 3 mol / L hydrochloric acid solution, react at a constant temperature of 80°C for 30 minutes to obtain slurry 1; the ratio of the mussel cooking liquid to the hydrochloric acid solution is 20:1.
[0050] 2. Calcium chloride was added to the acid extract of the mussel cooking liquid, the temperature was adjusted to 50° C., and the reaction was carried out for 90 minutes to obtain slurry 2; the solid-liquid ratio of the calcium chloride to the acid extract was 530 mL / g.
[0051] 3. The slurry was centrifuged at 3000 rpm for 10 min and filtered to remove insoluble matter. The filtrate was filtered using a 50 nm ceramic membrane, and then the retentate was ultrafiltered using a 1000 Da ultrafiltration membrane. The filtrate was compounded with glycerol, polyol, and preservative to obtain marine bioactive substances (this step is the same as in Example 1).
[0052] Comparative Example 2
[0053] The difference between Comparative Example 2 and Example 1 is that calcium gluconate is used instead of oyster shells to prepare the slurry in Comparative Example 2. The preparation method of the marine bioactive substance is as follows:
[0054] 1. Take mussel cooking liquid and 3 mol / L hydrochloric acid solution, react at a constant temperature of 80℃ for 30 minutes to obtain slurry 1. The ratio of mussel cooking liquid to hydrochloric acid solution is 20:1.
[0055] 2. Calcium gluconate was added to the acid extract of the mussel cooking liquid, the temperature was adjusted to 50° C., and the reaction was carried out for 90 minutes to obtain slurry 2; the solid-liquid ratio of the calcium gluconate to the acid extract was 530 mL / g.
[0056] 3. The slurry was centrifuged at 3000 rpm for 10 min and filtered to remove insoluble matter. The filtrate was filtered using a 50 nm ceramic membrane, and then the retentate was ultrafiltered using a 1000 Da ultrafiltration membrane. The filtrate was compounded with glycerol, polyol, and preservative to obtain marine bioactive substances (this step is the same as in Example 1).
[0057] Comparative Example 3
[0058] Comparative Example 3 differs from Example 1 in that taurine is used instead of mussel cooking liquid for treatment. The preparation method of the marine bioactive substance is as follows:
[0059] 1. Crush the oyster shells into small pieces and grind them in a high-speed grinder. Pass the crushed shell powder through a 100-mesh sieve to obtain oyster shell powder.
[0060] 2. Take oyster shell powder, add 3mol / L hydrochloric acid solution and an appropriate amount of purified water, and react at a constant temperature of 35°C for 80 minutes. The liquid-solid ratio of 3mol / L hydrochloric acid solution to oyster shell powder is 6ml / g, and the liquid-solid ratio of purified water to oyster shell powder is 50ml / g to obtain slurry one.
[0061] 3. Adjust the temperature of slurry 1 to 50°C, add taurine and react for 90 minutes to obtain slurry 2. The solid-liquid ratio of taurine to slurry 1 is 10 ml / g.
[0062] 4. Centrifuge at 3000 rpm for 10 min and filter to remove insoluble matter. The filtrate is filtered using a 50 nm ceramic membrane, and the retentate is then ultrafiltered using a 1000 Da filter. The filtrate is then compounded with glycerol, polyol, and preservative to obtain marine bioactive substances (this step is the same as in Example 1).
[0063] Comparative Example 4
[0064] Comparative Example 4 differs from Example 1 in that L-aspartic acid is used instead of mussel cooking liquid for treatment. The preparation method of the marine bioactive substance is as follows:
[0065] 1. Crush the oyster shells into small pieces and grind them in a high-speed grinder. Pass the crushed shell powder through a 100-mesh sieve to obtain oyster shell powder.
[0066] 2. Take oyster shell powder, add 3mol / L hydrochloric acid solution and an appropriate amount of purified water, and react at a constant temperature of 35°C for 80 minutes. The liquid-solid ratio of 3mol / L hydrochloric acid solution to oyster shell powder is 6ml / g, and the liquid-solid ratio of purified water to oyster shell powder is 50ml / g to obtain slurry one.
[0067] 3. Adjust the temperature of slurry 1 to 50°C, add L-aspartic acid and react for 90 minutes to obtain slurry 2; the solid-liquid ratio of the L-aspartic acid to slurry 1 is 10 ml / g.
[0068] 4. The slurry was centrifuged at 3000 rpm to remove insoluble matter. The filtrate was filtered using a 50 nm ceramic membrane. The retentate was ultrafiltered using a 1000 Da ultrafiltration membrane. The filtrate was compounded with glycerol, polyol, and preservative to obtain marine bioactive substances (this step is the same as in Example 1).
[0069] Comparative Example 5
[0070] The difference between Comparative Example 5 and Example 1 is that the slurry 3 of Comparative Example 5 is not subjected to the ceramic membrane and ultrafiltration impurity removal process, that is, glycerol, polyol and preservative are directly compounded after removing the insoluble matter, and the other aspects are the same.
[0071] Comparative Example 6
[0072] The difference between Comparative Example 6 and Example 1 is that, in Comparative Example 6, oyster shells are not used, and trypsin is directly used to enzymatically hydrolyze the mussel cooking liquid.
[0073] The preparation method of marine bioactive substances is as follows:
[0074] The mussel cooking liquid was taken, and trypsin (enzyme activity of 1000U / mL) was added in an enzyme amount of 0.5%. The reaction was carried out at a constant temperature of 37°C for 30 minutes, and then the enzyme was inactivated at 95°C for 10 minutes. The mixture was then centrifuged at 3000rpm for 10 minutes and filtered to remove insoluble matter. The filtrate was then filtered using a 50nm ceramic membrane, and the retentate was ultrafiltered using a 1000Da ultrafiltration membrane to obtain a filtrate, which was then compounded with glycerol, polyol, and preservative to prepare marine bioactive substances.
[0075] Comparative Example 7
[0076] The difference between Comparative Example 7 and Example 1 is that, in Comparative Example 7, oyster shells are not used, and papain is directly used to enzymatically hydrolyze the mussel cooking liquid.
[0077] The preparation method of marine bioactive substances is as follows:
[0078] The mussel cooking liquid was taken, papain (enzyme activity of 500 U / mL) was added, and the enzyme addition amount was 0.5%. The reaction was carried out at a constant temperature of 37°C for 30 minutes, and then the enzyme was inactivated at 95°C for 10 minutes. The mixture was then centrifuged at 3000 rpm for 10 minutes and filtered to remove insoluble matter. The filtrate was then filtered using a 50 nm ceramic membrane, and the retentate was then ultrafiltered using a 1000 Da ultrafiltration membrane to obtain a filtrate, which was then compounded with glycerol, polyol, and preservative to prepare a marine bioactive substance.
[0079] Comparative Example 8
[0080] Taurine 0.8 mol / L, calcium chloride 0.4 mol / L, react at 80°C water bath temperature for 30 min to obtain the active substance.
[0081] Test Example 1
[0082] The properties and stability of the active ingredients of the present invention were observed to evaluate the stability of the product properties.
[0083] The properties (color, clarity, odor) of the active ingredients prepared in Examples 1 to 4 and Comparative Examples 1 to 8 were observed, and the samples were placed in a 40° C. thermostat for 1 month for stability observation.
[0084] Table 1 Stability results of different active ingredients
[0085]
[0086] As shown in Table 1, the active ingredients of Examples 1-4 and Comparative Examples 1-8 have good appearance. Comparative Examples 6 and 7, which do not use acid, have a distinct fishy odor. This is because the main sources of seafood fishy odor are terpenes, amines, and pyridines. The acid used in the processes of Examples 1-4 and Comparative Examples 1-5 can neutralize the weakly alkaline amines, causing an acid-base neutralization reaction and converting the amines into ammonium salts. Ammonium salts are odorless, thus achieving the purpose of removing the fishy odor.
[0087] Furthermore, by comparing the stability results, it can be seen that the active ingredients of Examples 1 to 4, Comparative Examples 1 to 4, and 6 to 8 have good stability, while the material of Comparative Example 5 has precipitation. Seafood inevitably contains various small molecular substances such as salts or incompletely hydrolyzed macromolecular substances. If stored for a long time, these impurities will slowly polymerize and precipitate. In step 3, the ceramic membrane and microfiltration membrane are used to concentrate the material to remove impurities, which is helpful to the stability of the product.
[0088] Test Example 2
[0089] Hyaluronic acid not only has a moisturizing effect, but also helps maintain the skin's barrier function and reduces the damage to the skin by external irritants. When the skin barrier is damaged, the supplementation of hyaluronic acid can help repair the damaged stratum corneum, enhance the skin's defense ability, and regulate the composition of the extracellular matrix, promote the synthesis and remodeling of the extracellular matrix, thereby accelerating the repair of the skin. Therefore, the active ingredients of Examples 1 to 4, Comparative Examples 1 to 4, and 6 to 8 were tested for in vitro keratinocyte hyaluronic acid HA content to evaluate their skin evaluation and repair function.
[0090] The active ingredient was diluted with purified water to 1.25% and the efficacy of promoting the hyaluronic acid HA content in keratinocytes in the sample was determined as follows:
[0091] (1) Inoculation with human keratinocytes HACAT: Inoculate cells into a 96-well plate at a certain cell density and culture in a CO2 incubator for 24±2 hours.
[0092] (2) Dosing: When the cell plating rate in the 96-well plate reaches 40% to 60%, discard the culture medium in the 96-well plate and begin dosing. Add 200 μL of culture medium containing the test substance (1.25%) to the test wells. After dosing, place the 96-well plate in a CO2 incubator and incubate for 24 h ± 2 h.
[0093] (3) Testing: After the sample has been cultured for a period of time, collect 200 μL of the cell culture supernatant into a 1.5 mL sterile centrifuge tube and store it in a -80°C ultra-low temperature freezer. Perform the assay according to the instructions of the hyaluronic acid (HA) enzyme-linked immunosorbent assay (ELISA) kit and calculate the HA content and HA upregulation rate.
[0094]
[0095] Table 2 Repair function test results of different active substances
[0096]
[0097]
[0098] As can be seen from Table 2, although the active ingredients prepared from inorganic calcium and organic calcium (Comparative Examples 1 and 2) and chemically synthesized taurine and L-aspartic acid (Comparative Examples 3 and 4) also have the function of promoting cellular hyaluronic acid secretion, the hyaluronic acid upregulation rate is significantly lower than that of the active ingredients of Examples 1 to 4. This shows that the active ingredients prepared from seafood processing by-products of the present invention can significantly promote the synthesis of hyaluronic acid, which helps to exert moisturizing and repair effects.
[0099] Then, from the test results of Comparative Examples 6 and 7, it can be seen that Comparative Examples 6 and 7 do not significantly promote the ability of keratinocytes to secrete hyaluronic acid. It can be seen that in the process of extracting active ingredients from mussel cooking liquid, compared with enzymatic hydrolysis, the active ingredients prepared by the acid extraction process can significantly promote the synthesis of hyaluronic acid and have better skin barrier repair effect.
[0100] Test Example 3
[0101] The active ingredients prepared in Examples 1 to 4 and Comparative Examples 1 to 4 and 8 were tested for their cell migration promoting efficacy to evaluate their skin repair function. The specific method is as follows:
[0102] HACAT human immortalized keratinocytes in logarithmic growth phase and in good condition were digested with trypsin to prepare a single-cell suspension. The cells were seeded into a 96-well plate. When the cells were nearly confluent, they were streaked evenly with a 200 μL pipette tip. The blank group was replaced with complete medium supplemented with 1% serum, while the sample group was replaced with complete medium supplemented with the test substance (0.1%) and 1% serum.
[0103] Observation and Photography: Take a photo of the scratched area under a 4X microscope objective (at 0 hours of incubation). Then, place the culture plate in a 37°C, 5% CO2 incubator and continue incubation. Observe cell migration for 18-24 hours. Remove the culture plate at a predetermined time point (e.g., 23 hours) and take a photo of the same location. Calculate the scratched area using ImageJ software and calculate the migration rate using the following formula:
[0104]
[0105] Table 3 Cell migration efficacy test results
[0106] sample 23h average migration rate (%) Blank group 29.24 Example 1 33.23 Example 2 34.56 Example 3 33.14 Example 4 31.24 Comparative Example 1 32.84 Comparative Example 2 28.53 Comparative Example 3 25.15 Comparative Example 4 24.26 Comparative Example 8 28.48
[0107] As can be seen from Table 3, the active substances prepared using seafood processing by-products as the central calcium ion and the active substances prepared using inorganic calcium and organic calcium as the central ion calcium sources (Comparative Examples 1 and 2) also have the function of promoting cell migration, but the average migration rate over 23 hours is substantially lower than that of the active substances of Examples 1 to 3. Furthermore, the average migration rates of Comparative Examples 3, 4, and 8 are significantly lower than those of the active substances of Examples 1 to 3. This indicates that the active substances of Comparative Examples 3, 4, and 8 do not have the ability to significantly promote cell migration.
[0108] At the same time, by Figures 1-2 It can be seen that after the cells were treated with the active ingredients of Examples 1 to 3, the scratches continued to shrink as the cells continued to migrate, proving that the active ingredients prepared using seafood processing by-products as central calcium ions have good skin barrier repair effects.
[0109] Test Example 4
[0110] The active ingredients prepared in Examples 1 to 4 and Comparative Examples 1 to 4 and 8 were subjected to the eye irritation / corrosiveness test using a chick embryo chorioallantoic membrane to evaluate their mildness. The specific method is as follows:
[0111] Nine-day-old chicken embryos were used. The eggshells in the air cell area were peeled off, and the eggshell membranes were removed. The chorioallantoic sac was recorded with a camera. The test substance was applied to a thin layer of plastic film and covered with the chorioallantoic sac. After the test substance was applied to the chorioallantoic sac, it was gently rinsed with saline. The damage to the chorioallantoic vasculature was recorded with a camera and scored using the reaction time method.
[0112] Table 4 Mildness test judgment standard
[0113] Stimulus score Irritation classification IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 Moderate irritation IS≥10 Strong irritant / corrosive
[0114] Table 5 Mildness test results of different active ingredients
[0115]
[0116]
[0117] As shown in Table 5, the marine bioactives prepared in Examples 1 to 4 have relatively good mildness, with an IS value of 0, while Comparative Examples 1, 2, and 8 have slight irritation. This indicates that the marine bioactives prepared in the present invention using seafood processing by-products as the central calcium ion are milder than inorganic calcium and organic calcium.
[0118] In summary, the marine bioactive substances prepared from mussel cooking liquid and oyster shells, which are by-products of seafood processing, have significant advantages in promoting hyaluronic acid HA in keratinocytes and promoting cell migration, and are very helpful in repairing skin barrier properties.
[0119] Moreover, the present invention uses seafood processing by-products as raw materials, which are safe and non-toxic. It not only meets the market demand for natural and mild skin care, but also increases the added value of seafood processing by-products, reduces environmental pollution, and provides new ideas for the research and development of repair functional raw materials.
[0120] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.
Claims
1. A method for preparing marine bioactive substances with repairing effects using seafood processing by-products, characterized in that: The method comprises the following steps: S1. Take oyster shell powder and perform a first acid treatment to obtain a slurry; S2. Take the mussel cooking liquid for a second acid treatment to obtain a slurry II; S3. The slurry 1 and slurry 2 were mixed and reacted at 45 to 55 ° C for 80 to 100 min to obtain slurry 3; S4. The slurry is centrifuged and filtered to obtain the filtrate, which is filtered through a 50 nm ceramic membrane and a 1000 Da ultrafiltration membrane in sequence. The filtrate is compounded with glycerol, polyols and preservatives to produce marine bioactive substances.
2. The method for preparing marine bioactive substances with repairing effects using seafood processing by-products according to claim 1, characterized in that: In step S1, the first acid treatment is to add oyster shell powder to an acid solution and water, and react at a constant temperature of 35°C for 80 minutes to obtain slurry 1; the oyster shell powder is oyster shells crushed and passed through a 100-mesh sieve.
3. The method for preparing marine bioactive substances with repairing effects using seafood processing by-products according to claim 2, characterized in that: In step S1, the liquid-solid ratio of the acid solution to the oyster shell powder is 6 mL / g or 10 mL / g; the liquid-solid ratio of the water to the oyster shell powder is 50 mL / g.
4. The method for preparing marine bioactive substances with repairing effects using seafood processing byproducts according to claim 1, characterized in that: In step S2, the second acid treatment is to take mussel cooking liquid and acid solution, react at a constant temperature of 80°C for 30 minutes to obtain slurry 2; the volume ratio of the mussel cooking liquid to the acid solution is 20:1 or 100:
1.
5. The method for preparing marine bioactive substances with repairing effects using seafood processing by-products according to claim 1, characterized in that: In step S3, the ratio of the slurry 2 to the slurry 1 is 30:1 or 45:
1.
6. The method for preparing marine bioactive substances with repair efficacy using seafood processing by-products according to claim 1, characterized in that: The first acid treatment and the second acid treatment use a hydrochloric acid solution; Optionally, in the turbid liquid system treated with the first acid and the second acid, H + The concentration is 3 mol / L.
7. The marine bioactive substance obtained by the method for preparing marine bioactive substances with repair efficacy using seafood processing by-products according to any one of claims 1 to 6.
8. Use of the marine bioactive substance according to claim 7 in the preparation of cosmetic products.
9. The use according to claim 8, characterized in that The dosage form of the cosmetic product is water, emulsion, cream, oil, spray, powder or gel.