Sleep-promoting pitaya fermented liquid complex and preparation method thereof

Abstract

The present invention relates to the field of medical technology, and more particularly to a sleep-promoting pitaya fermentation liquor complex and a preparation method thereof, comprising the following raw materials in parts by weight: 65-70 parts of red pitaya fermentation liquor, 3-5 parts of microencapsulated lavender oil powder, 2-4 parts of synergistic microcapsule powder, 1.5-2.5 parts of a sweetness regulator, and 0.3-0.5 parts of a stabilizer; wherein the microencapsulated lavender oil powder is prepared with casein peptide-soybean lecithin as a shell material and lavender oil as a core material; the synergistic microcapsule powder is formed by cross-linking tannic acid with chitosan-carboxymethyl cellulose as a shell material and theanine, 5-hydroxytryptophan and piperine as a core material. The sleep-promoting pitaya fermentation liquor complex is prepared by processes such as homogenization, filling, and sterilization. The complex of the present invention can effectively help sleep, and the preparation method is simple, with broad application prospects.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to a sleep-promoting pitaya fermentation liquid complex and a preparation method thereof. Background Art

[0002] In modern society, sleep disorders have become a common problem affecting human health. Long-term insomnia or poor sleep quality not only reduces quality of life but can also lead to cardiovascular and cerebrovascular diseases and weakened immunity. Consequently, more and more people are interested in foods and health supplements that improve sleep quality. Currently, many sleep-aid products on the market rely primarily on melatonin or chemical sedatives, which carry the risk of dependency and side effects. Natural plant ingredients, such as lavender essential oil, while capable of modulating GABA receptors, suffer from rapid metabolism and low bioavailability.

[0003] Chinese patent application number CN202210054401.X discloses a tranquilizing and sleep-inducing composition, its preparation method and application. The tranquilizing and sleep-inducing composition of the invention is composed of the following raw materials in parts by mass: 10-30 parts of jujube seed powder, 10-30 parts of lily peptide, 10-15 parts of corn oligopeptide, 3-5 parts of sorbitol, 5-13 parts of γ-aminobutyric acid, 10-20 parts of passion fruit powder, 0.1-0.3 parts of sweetener, and 1-2.5 parts of acidulant. The tranquilizing and sleep-inducing composition provided by the present invention can regulate the entire sleep process, allowing insomniacs to fall asleep quickly, and making people still full of energy after waking up, without becoming drowsy. Chinese patent application number CN202411166075.7 discloses a tranquilizing and sleep-inducing composition containing agarwood leaves, its preparation method, use, and health tea. The tranquilizing and sleep-inducing composition comprises the following ingredients by weight: 10-18 parts agarwood leaves; 5-12 parts Alpinia oxyphylla seeds; 8-16 parts Polygonum multiflorum vine; 8-16 parts Platycladi seed; and 3-7 parts theanine. However, these two inventions simply combine the ingredients without fully considering the interactions between these ingredients, making it difficult to determine whether this combination truly offers advantages over single ingredients or traditional combinations.

[0004] Therefore, developing a sleep-aiding complex based on pitaya fermentation liquid can not only integrate different functional ingredients and play a complementary role, but also help meet the market demand for natural, safe and effective sleep-aiding products, providing users with a better sleep experience. Summary of the Invention

[0005] To address the above issues, the present invention provides a sleep-enhancing pitaya fermentation broth complex and its preparation method. By utilizing the synergistic cross-linking effect of casein peptides and soy lecithin, a stable shell material solution is formed through transglutaminase catalysis, resulting in microencapsulated lavender oil powder. A synergistic microcapsule powder with a synergistic sedative effect is obtained by precisely blending the chitosan-carboxymethyl cellulose composite shell material with theanine, 5-hydroxytryptophan, and piperine. Finally, combined with the natural active ingredients of red pitaya fermentation broth, a sleep-enhancing pitaya fermentation broth complex with excellent sleep-promoting efficacy is obtained through high-pressure homogenization and sterilization.

[0006] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are:

[0007] A sleep-enhancing pitaya fermentation liquid complex, comprising the following raw materials in parts by weight: 65-70 parts of red pitaya fermentation liquid, 3-5 parts of microencapsulated lavender oil powder, 2-4 parts of synergistic microcapsule powder, 1.5-2.5 parts of a sweetness regulator, and 0.3-0.5 parts of a stabilizer;

[0008] The preparation method of the microencapsulated lavender oil powder is as follows:

[0009] Step S1, dissolving casein peptide and soybean lecithin in deionized water, adjusting the pH to 6.0-7.0, adding transglutaminase, and cross-linking at 40-50° C. for 30-60 minutes to obtain a shell material solution;

[0010] Step S2: adding lavender essential oil to the shell material solution, homogenizing under high pressure, and obtaining microencapsulated lavender oil powder through post-processing.

[0011] Furthermore, the mass ratio of the casein peptide to soybean lecithin in step S1 is 1:1.5-2.5, the amount of casein peptide added to deionized water is 0.02-0.03 g / mL, and the amount of transglutaminase added per gram of casein peptide is 9-10 U / g.

[0012] Furthermore, in step S2, the mass volume ratio of the lavender essential oil to the shell material solution is 0.01-0.025 g / mL.

[0013] Furthermore, the preparation method of the synergistic microcapsule powder is as follows:

[0014] Step a, dissolving chitosan in acetic acid solution to form a chitosan solution, dissolving carboxymethyl cellulose in deionized water, then adding the chitosan solution, and mixing uniformly to obtain a shell material solution;

[0015] Step b, mixing the theanine solution, 5-hydroxytryptophan solution, and piperine solution, and ultrasonicating for 10-20 minutes to obtain a core material solution;

[0016] Step c: mixing the shell material solution and the core material solution, adjusting the pH to 4.5-5.0, stirring at room temperature for 20-40 minutes, adding tannic acid solution, stirring at room temperature for 4-5 hours, and obtaining synergistic microcapsule powder through post-treatment.

[0017] Furthermore, the concentration of the chitosan solution in step a is 1-3%, the amount of carboxymethyl cellulose added to deionized water is 1-1.5 g / 100 ml, and the mass ratio of chitosan to carboxymethyl cellulose is 2-2.5:1.

[0018] Furthermore, the mass concentration of theanine solution in step b is 0.1-0.2 g / 100 mL, the mass concentration of 5-hydroxytryptophan solution is 0.05-0.1 g / 100 mL, the mass concentration of piperine solution is 0.01-0.02 g / 100 mL, and the mass ratio of theanine, 5-hydroxytryptophan, and piperine is 4-6:2-3:1.

[0019] Furthermore, in step c, the volume ratio of the shell material solution to the core material solution is 1:3-4, and the mass volume ratio of tannic acid to the shell material solution is 0.0075-0.015 / 100 mL.

[0020] Furthermore, the sweetness regulator is a mixture of erythritol and mogroside.

[0021] Furthermore, the stabilizer is a mixture of high-ester pectin and propylene glycol alginate.

[0022] The present invention also provides a method for preparing a sleep-promoting pitaya fermented liquid complex, comprising the following steps:

[0023] (1) Mixing the fermented pitaya liquid, microencapsulated lavender oil powder, synergistic microcapsule powder, sweetness regulator, and stabilizer to obtain a mixture;

[0024] (2) The mixture obtained in step (1) is homogenized, and then filled, sterilized, and cooled.

[0025] The present invention has the following beneficial effects:

[0026] The present invention first prepares microencapsulated lavender oil powder and synergistic microcapsule powder through microcapsule technology. The preparation of microencapsulated lavender oil powder adopts casein peptide and soybean lecithin as shell material raw materials, and forms a stable shell material structure through transglutaminase cross-linking, which effectively protects the volatile components of lavender essential oil and prevents its loss during storage and use. At the same time, the microencapsulation of lavender essential oil improves its stability in the product and prolongs its sustained release performance, so that it can continue to exert a calming and sleep-inducing effect after ingestion; the synergistic microcapsule powder first uses a variety of ingredients with neuromodulatory effects such as theanine, 5-hydroxytryptophan and piperine as core materials, and prepares the shell material through the synergistic effect of chitosan and carboxymethyl cellulose, forming a stable microcapsule structure, ensuring the stability and effectiveness of these active ingredients in the product.

[0027] Microencapsulated lavender oil powder and synergistic microcapsule powder are compounded with red pitaya fermentation broth, sweetness modifiers, stabilizers, and other raw materials to prepare a sleep-enhancing pitaya fermentation broth complex. Through homogenization, filling, and sterilization, the uniformity and safety of the sleep-enhancing pitaya fermentation broth complex are ensured, thereby improving the product's shelf life and user experience. Therefore, through a scientific formula and advanced technology, the present invention prepares a sleep-enhancing pitaya fermentation broth complex that effectively improves sleep quality and has safe ingredients. DETAILED DESCRIPTION

[0028] The following will be combined with the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the embodiments described are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0029] The raw materials used in the following examples are all common commercial products. Casein peptide has an effective ingredient content of 99%, a particle size of 80-100 mesh, and is purchased from Shanxi Zhongnuo Biotechnology Co., Ltd.; soybean lecithin has an effective ingredient content of 99%, a particle size of 120 mesh, and a drying loss (w / %) of 0.82, and is purchased from Hubei Haijia Biotechnology Co., Ltd.; transglutaminase has an effective ingredient content of 99%, an enzyme activity preservation rate of 99%, and an arsenic content of 0.01%, and is purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.; lavender essential oil has an effective ingredient content of 99%, a density of 0.882 g / mL, and is purchased from Wuhan Kemik Biomedical Technology Co., Ltd.; chitosan has a deacetylation degree of 0.22%, a viscosity of 21 cps, and complies with GB 29941-2013 standard, purchased from Sichuan Huanxu Biotechnology Co., Ltd.; carboxymethyl cellulose viscosity 3100mPa.s, active ingredient content 99.7%, in line with GB1886.232-2016 standard, purchased from Shandong Xinxiong Biotechnology Co., Ltd.; theanine active ingredient content 99.8%, pH value 5.45, purchased from Shandong Xinxiong Biotechnology Co., Ltd.; mogroside active ingredient content 99%, particle size 120 mesh, purchased from Shandong Xinxiong Biotechnology Co., Ltd.; high ester pectin active ingredient content 99%, molecular weight 20000-400000, purchased from Hefei Shengrun Biological Products Co., Ltd.; propylene glycol alginate viscosity 340mPa.s, active ingredient content 99%, in line with GB1886.226-2016 standard, purchased from Shaanxi Rankang Biotechnology Co., Ltd.; red pitaya fermentation liquid and its preparation method have been disclosed in the patent document with application number CN201810157407.3. Example

[0030] A sleep-enhancing pitaya fermentation liquid complex, comprising the following raw materials in parts by weight: 65 parts of red pitaya fermentation liquid, 3 parts of microencapsulated lavender oil powder, 2 parts of synergistic microcapsule powder, 1.5 parts of a sweetness regulator, and 0.3 parts of a stabilizer;

[0031] The preparation method of the microencapsulated lavender oil powder is as follows:

[0032] Step S1, dissolving casein peptide and soybean lecithin in deionized water, adjusting the pH value to 6.0-7.0 with 0.1M NaOH aqueous solution, adding transglutaminase, cross-linking at 45°C for 40 minutes, treating at 70°C for 10 minutes to inactivate the enzyme and terminate the reaction to obtain a shell material solution, wherein the mass ratio of casein peptide to soybean lecithin is 1:2, the amount of casein peptide added in deionized water is 0.025g / mL, and the amount of transglutaminase added per gram of casein peptide is 9.5U / g;

[0033] Step S2, adding lavender essential oil dropwise to the shell material solution at a dropping rate of 2 mL / min while stirring, and after the dropwise addition is completed, homogenizing under high pressure for 3 times at a pressure of 60 MPa and a homogenization temperature of 25-30°C, spray drying for 20 minutes under the conditions of an inlet air temperature of 160°C, an outlet air temperature of 75°C, an atomization pressure of 0.3 MPa, and a feed rate of 10 mL / min to obtain microencapsulated lavender oil powder, wherein the mass volume ratio of lavender essential oil to shell material solution is 0.02 g / mL.

[0034] The preparation method of the synergistic microcapsule powder is as follows:

[0035] Step a, dissolving chitosan in 1% acetic acid solution to form a chitosan solution, adjusting the pH of the chitosan solution to 4.5-5.0 with a 1M NaOH aqueous solution, dissolving carboxymethyl cellulose in deionized water, adjusting the pH to 5.5-6.5 in the same manner, then adding the chitosan solution, and mixing uniformly to obtain a shell material solution, wherein the concentration of the chitosan solution is 2%, the amount of carboxymethyl cellulose added to the deionized water is 1.3g / 100ml, and the mass ratio of chitosan to carboxymethyl cellulose is 2.2:1;

[0036] Step b, mixing the theanine aqueous solution, the 5-hydroxytryptophan aqueous solution, and the piperine solution, and ultrasonicating for 15 minutes to obtain a core material solution, wherein the mass concentration of the theanine aqueous solution is 0.15 g / 100 mL, the mass concentration of the 5-hydroxytryptophan aqueous solution is 0.08 g / 100 mL, and the mass concentration of the piperine solution is 0.015 g / 100 mL. The piperine solution is a mixed solvent of ethanol and water with a volume ratio of 1:9, and the mass ratio of theanine, 5-hydroxytryptophan, and piperine is 5:2.5:1;

[0037] Step c, mixing the shell material solution and the core material solution, adjusting the pH to ‌4.5-5.0 with a 1M NaOH aqueous solution or a 1M HCl aqueous solution, stirring at room temperature for 30 minutes, adding a 2% tannic acid solution dropwise at a rate of 0.5 mL / min, stirring in the dark for 4.5 hours at room temperature, centrifuging at 4000 rpm for 10 minutes, washing the centrifuged product twice with deionized water, dispersing the washed product in a 10% sucrose aqueous solution, pre-freezing at −40° C. for 12 hours, and then drying at −50° C. and a vacuum degree of ≤10 Pa for 24 hours to obtain a synergistic microcapsule powder, wherein the volume ratio of the shell material solution to the core material solution is 1:3.5, the mass-to-volume ratio of the tannic acid to the shell material solution is 0.01 / 100 mL, and the mass ratio of sucrose to the washed product is 0.8-1:9.

[0038] The sweetness regulator is a mixture of erythritol and mogroside in a mass ratio of 3:1, and the stabilizer is a mixture of high-ester pectin and propylene glycol alginate in a mass ratio of 1:1.

[0039] A method for preparing a sleep-promoting pitaya fermented liquid complex comprises the following steps:

[0040] (1) Mixing the fermented pitaya liquid, microencapsulated lavender oil powder, synergistic microcapsule powder, sweetness regulator, and stabilizer to obtain a mixture;

[0041] (2) The mixture obtained in step (1) is homogenized at 30 MPa and a temperature of 25-30°C for 10 minutes, and then filled, sterilized, and cooled. Example

[0042] A sleep-enhancing pitaya fermented liquid complex is composed of the following raw materials in parts by weight: 70 parts of red pitaya fermented liquid, 5 parts of microencapsulated lavender oil powder, 4 parts of synergistic microcapsule powder, 2.5 parts of a sweetness regulator, and 0.5 parts of a stabilizer; the remaining ingredients are the same as in Example 1. Example

[0043] A sleep-enhancing pitaya fermented liquid complex is composed of the following raw materials in parts by weight: 67 parts of red pitaya fermented liquid, 4 parts of microencapsulated lavender oil powder, 3 parts of synergistic microcapsule powder, 2 parts of a sweetness regulator, and 0.54 parts of a stabilizer; the remaining ingredients are the same as in Example 1. Example

[0044] A sleep-enhancing pitaya fermentation liquid complex, comprising the following raw materials in parts by weight: 67 parts of red pitaya fermentation liquid, 4 parts of microencapsulated lavender oil powder, 3 parts of synergistic microcapsule powder, 2 parts of a sweetness regulator, and 0.54 parts of a stabilizer;

[0045] The preparation method of the microencapsulated lavender oil powder is as follows:

[0046] Step S1, dissolving casein peptide and soybean lecithin in deionized water, adjusting the pH value to 6.0-7.0 with 0.1M NaOH aqueous solution, adding transglutaminase, cross-linking at 50°C for 60 minutes, treating at 70°C for 10 minutes to inactivate the enzyme and terminate the reaction to obtain a shell material solution, wherein the mass ratio of casein peptide to soybean lecithin is 1:2.5, the amount of casein peptide added in deionized water is 0.03g / mL, and the amount of transglutaminase added per gram of casein peptide is 10U / g;

[0047] Step S2, adding lavender essential oil dropwise to the shell material solution at a dropping rate of 2 mL / min while stirring, and after the dropwise addition is completed, high-pressure homogenization is performed three times at a pressure of 60 MPa and a homogenization temperature of 25-30°C, and spray drying is performed for 20 minutes under the conditions of an inlet air temperature of 160°C, an outlet air temperature of 75°C, an atomization pressure of 0.3 MPa, and a feed rate of 10 mL / min to obtain microencapsulated lavender oil powder, wherein the mass volume ratio of lavender essential oil to shell material solution is 0.025 g / mL.

[0048] The preparation method of the synergistic microcapsule powder is as follows:

[0049] Step a, dissolving chitosan in 1% acetic acid solution to form a chitosan solution, adjusting the pH of the chitosan solution to 4.5-5.0 with a 1M NaOH aqueous solution, dissolving carboxymethyl cellulose in deionized water, adjusting the pH to 5.5-6.5 in the same manner, then adding the chitosan solution, and mixing uniformly to obtain a shell material solution, wherein the concentration of the chitosan solution is 3%, the amount of carboxymethyl cellulose added to the deionized water is 1.5g / 100ml, and the mass ratio of chitosan to carboxymethyl cellulose is 2.5:1;

[0050] Step b, mixing the theanine aqueous solution, the 5-hydroxytryptophan aqueous solution, and the piperine solution, and ultrasonicating for 20 minutes to obtain a core material solution, wherein the mass concentration of the theanine aqueous solution is 0.2 g / 100 mL, the mass concentration of the 5-hydroxytryptophan aqueous solution is 0.1 g / 100 mL, and the mass concentration of the piperine solution is 0.02 g / 100 mL. The piperine solution is a mixed solvent of ethanol and water with a volume ratio of 1:9, and the mass ratio of theanine, 5-hydroxytryptophan, and piperine is 6:3:1;

[0051] Step c, mixing the shell material solution and the core material solution, adjusting the pH to 4.5-5.0 with a 1M NaOH aqueous solution or a 1M HCl aqueous solution, stirring at room temperature for 40 minutes, adding a 2% tannic acid solution dropwise at a rate of 0.5 mL / min, stirring in the dark at room temperature for 5 hours, centrifuging at 4000 rpm for 10 minutes, washing the centrifuged product twice with deionized water, dispersing the washed product in a 10% sucrose aqueous solution, pre-freezing at −40° C. for 12 hours, and then drying at −50° C. and a vacuum degree of ≤10 Pa for 24 hours to obtain a synergistic microcapsule powder, wherein the volume ratio of the shell material solution to the core material solution is 1:4, the mass-to-volume ratio of the tannic acid to the shell material solution is 0.015 / 100 mL, and the mass ratio of sucrose to the washed product is 0.8-1:9.

[0052] The rest is the same as Example 1.

[0053] A sleep-enhancing pitaya fermentation liquid complex, comprising the following raw materials in parts by weight: 30 parts of red pitaya fermentation liquid, 1 part of microencapsulated lavender oil powder, 1 part of synergistic microcapsule powder, 1.5 parts of a sweetness regulator, and 0.3 parts of a stabilizer;

[0054] The preparation method of the microencapsulated lavender oil powder is as follows:

[0055] Step S1, dissolving casein peptide and soybean lecithin in deionized water, adding transglutaminase, cross-linking at room temperature for 40 minutes, treating at 70°C for 10 minutes to inactivate the enzyme and terminate the reaction to obtain a shell material solution, wherein the mass ratio of casein peptide to soybean lecithin is 1:1, the amount of casein peptide added to deionized water is 0.025 g / mL, and the amount of transglutaminase added per gram of casein peptide is 9.5 U / g;

[0056] Step S2, adding lavender essential oil dropwise to the shell material solution at a dropping rate of 2 mL / min while stirring, and after the dropwise addition is completed, homogenizing under high pressure for 3 times at a pressure of 60 MPa and a homogenization temperature of 25-30°C, spray drying for 20 minutes under the conditions of an inlet air temperature of 160°C, an outlet air temperature of 75°C, an atomization pressure of 0.3 MPa, and a feed rate of 10 mL / min to obtain microencapsulated lavender oil powder, wherein the mass volume ratio of lavender essential oil to shell material solution is 0.1 g / mL.

[0057] The preparation method of the synergistic microcapsule powder is as follows:

[0058] Step a, dissolving chitosan in 1% acetic acid solution to form a chitosan solution, adjusting the pH of the chitosan solution to 4.5-5.0 with a 1M NaOH aqueous solution, dissolving carboxymethyl cellulose in deionized water, adjusting the pH to 5.5-6.5 in the same manner, then adding the chitosan solution, and mixing uniformly to obtain a shell material solution, wherein the concentration of the chitosan solution is 2%, the amount of carboxymethyl cellulose added to the deionized water is 1.3g / 100ml, and the mass ratio of chitosan to carboxymethyl cellulose is 1:1;

[0059] Step b, mixing the theanine aqueous solution, the 5-hydroxytryptophan aqueous solution, and the piperine solution, and ultrasonicating for 15 minutes to obtain a core material solution, wherein the mass concentration of the theanine aqueous solution is 0.15 g / 100 mL, the mass concentration of the 5-hydroxytryptophan aqueous solution is 0.08 g / 100 mL, and the mass concentration of the piperine solution is 0.015 g / 100 mL. The piperine solution is a mixed solvent of ethanol and water with a volume ratio of 1:9, and the mass ratio of theanine, 5-hydroxytryptophan, and piperine is 1:1:1;

[0060] Step c, mixing the shell material solution and the core material solution, stirring at room temperature for 30 minutes, adding a 2% tannic acid solution dropwise at a rate of 0.5 mL / min, stirring in the dark for 4.5 hours at room temperature, centrifuging at 4000 rpm for 10 minutes, washing the centrifuged product twice with deionized water, dispersing the washed product in a 10% sucrose aqueous solution, pre-freezing at −40° C. for 12 hours, and then drying at −50° C. and a vacuum degree of ≤10 Pa for 24 hours to obtain a synergistic microcapsule powder, wherein the volume ratio of the shell material solution to the core material solution is 1:1, the mass volume ratio of tannic acid to the shell material solution is 0.001 / 100 mL, and the mass ratio of sucrose to the washed product is 0.8-1:9.

[0061] The rest is the same as Example 1.

[0062] A sleep-aiding pitaya fermented liquid complex and a preparation method thereof, wherein microencapsulated lavender oil powder is replaced with lavender essential oil, and the rest is the same as in Example 1.

[0063] A sleep-aiding pitaya fermented liquid complex and a preparation method, wherein the synergistic microcapsule powder is replaced with theanine, and the rest is the same as in Example 1.

[0064] A sleep-aiding pitaya fermented liquid complex and a preparation method, wherein microencapsulated lavender oil powder is replaced with lavender essential oil, and synergistic microcapsule powder is replaced with theanine, and the rest is the same as in Example 1.

[0065] Clean, NIH-grade healthy adult mice, half male and half female, weighing 24-28g, were randomly divided into 11 groups (n=10 / group, half male and half female) after one week of adaptive feeding. These groups were designated as a blank control, model control, positive control, Example 1-4, and Comparative Example 1-4. Mice in the model control, positive control, Example 1-4, and Comparative Example 1-4 groups received intraperitoneal injections of 400 mg / kg of a PCPA suspension (PCPA suspension preparation: para-chlorophenylalanine powder was added to saline and ultrasonically dispersed for 20 minutes to obtain a 30 mg / ml PCPA suspension) for three consecutive days; the blank control group received the same dose of saline. Mice in the model control, positive control, Example 1-4, and Comparative Example 1-4 groups all displayed significant signs of circadian rhythm impairment, manifested by a significant decrease in daytime activity, lethargy, and increased aggressive behavior. These changes were particularly significant compared to the blank control group, fully demonstrating the effectiveness and success of the established insomnia model.

[0066] Mice in Example 1-4 and Comparative Example 1-4 groups were gavaged with the corresponding sample (the sleep-enhancing pitaya fermentation broth complex) for 15 consecutive days. The model control and blank control groups were fed an equal amount of distilled water. The positive control group was fed Yixin Ningshen tablets at a dose of 1.3 g / kg. Yixin Ningshen tablets were dissolved and dispersed in water for easy feeding. The human equivalent dose for Example 1-4 and Comparative Example 1-4 groups was administrated (the human dose is 10 g / day, calculated based on body surface area, with a feed volume of 0.5 mL per mouse). Twenty minutes after the last dose, sodium pentobarbital solution was injected intraperitoneally at a dose of 35 mg / kg, with an injection volume of 0.1 mL / 10 g based on mouse body weight. Immediately after injection, the mice were observed and recorded for sleep latency and sleep duration. The improvement in sleep rate was also observed at 11:00 PM. The test results are shown in Table 1.

[0067] Sleep was assessed using the loss of the righting reflex as an indicator of sleep. When placed in a dorsal recumbent position, mice were able to right themselves immediately. If they were unable to do so for more than 60 seconds, they were considered to have lost their righting reflex and entered sleep. Restoration of the righting reflex marked awakening. The time from injection of sodium pentobarbital to the loss of the righting reflex was defined as sleep latency; the time from loss of the righting reflex to its recovery was defined as sleep duration. At 11:00 PM, mice were observed for activity, and the proportion of inactive mice in each group was calculated. This percentage was then subtracted from the proportion in the model control group to determine the sleep improvement rate.

[0068] Table 1 Sleep index test results of mice in each group

[0069] Group Sleep time (min) Sleep latency (min) Sleep improvement rate (%) Blank control 36.8 8.0 / Model comparison 17.3 10.8 / Positive control 28.6 5.9 67.2 Example 1 39.1 3.5 79.1 Example 2 37.9 3.7 79.5 Example 3 38.5 3.2 80.0 Example 4 38.6 3.4 78.9 Comparative Example 1 34.4 4.4 74.3 Comparative Example 2 28.5 6.1 68.8 Comparative Example 3 27.8 5.9 66.8 Comparative Example 4 20.4 8.2 60.5

[0070] As can be seen from Table 1, compared with the examples, the complex prepared in Comparative Example 1 reduced the sleep time and sleep improvement rate of mice in the mouse experiment, and increased the sleep latency, indicating that the complex prepared in Comparative Example 1 is less effective in improving sleep quality than the examples. Therefore, the ratio of the sleep-enhancing pitaya fermented liquid complex proposed in the present invention is optimal.

[0071] As can be seen from Table 1, compared with the embodiment, the complex prepared in Comparative Example 2 has reduced sleep time and sleep improvement rate of mice in the mouse experiment, and improved sleep latency, indicating that the complex prepared in this comparative example has limited effect on improving sleep quality. This is because the microencapsulated lavender oil powder of Comparative Example 2 is replaced with lavender essential oil, and encapsulation can protect lavender oil from external environmental factors such as oxygen, light, temperature, etc. These factors can cause lavender oil to oxidize, volatilize or deteriorate, thereby reducing its sleep-aiding effect. Therefore, the microencapsulated lavender oil powder can improve the stability of lavender oil in the complex and extend the shelf life of the complex; the powdered microencapsulated lavender oil is easier to disperse evenly in the complex and is fully mixed with other raw materials, which helps to ensure the uniformity of the components in the complex, so that each complex can contain a relatively stable lavender oil content, thereby ensuring the consistency of the sleep-aiding effect.

[0072] As can be seen from Table 1, compared with the examples, the composite prepared in Comparative Example 3 reduced the sleep time and sleep improvement rate of mice in the mouse experiment, and increased the sleep latency, indicating that the composite prepared in this comparative example is not effective in improving sleep quality. This is because the synergistic microcapsule powder of Comparative Example 3 is replaced with theanine. The theanine, 5-hydroxytryptophan and piperine in the synergistic microcapsule powder have the effects of calming and relieving fatigue, promoting melatonin secretion and improving the bioavailability of the former two, respectively. Their synergistic effect can more effectively regulate the sleep cycle and improve sleep quality; the dense network formed by this chitosan / carboxymethyl cellulose shell material can block the degradation of active ingredients by oxygen, light and gastric acid; tannic acid, as a cross-linking agent, forms a Schiff base bond with the amino group of chitosan, significantly improving the strength of the shell material, thereby more effectively protecting the core material, so that theanine, 5-hydroxytryptophan and piperine can more effectively improve sleep quality.

[0073] As can be seen from Table 1, compared with the embodiment, the complex prepared in Comparative Example 4 has the lowest sleep time and sleep improvement rate of mice in the mouse experiment, and the highest sleep latency, indicating that the complex prepared in this comparative example has the worst effect on improving sleep quality. This is because the microencapsulated lavender oil powder in Comparative Example 4 is replaced by lavender essential oil, and the synergistic microcapsule powder is replaced by theanine. Due to the lack of microencapsulation technology, the lavender essential oil and active ingredients cannot be effectively protected, and will be degraded due to oxidation, light or temperature changes during storage, thereby reducing the shelf life of the sleep-aiding pitaya fermentation liquid complex; in addition, theanine and piperine have synergistic free radical scavenging ability, which can reduce the damage of oxidative stress to neurons, thereby prolonging the effective period of lavender essential oil in helping sleep and enhancing the sleep-aiding effect of the sleep-aiding pitaya fermentation liquid complex.

[0074] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A sleep-promoting pitaya fermentation liquid complex, characterized in that: The invention is composed of the following raw materials in parts by weight: 65-70 parts of red pitaya fermentation liquid, 3-5 parts of microencapsulated lavender oil powder, 2-4 parts of synergistic microcapsule powder, 1.5-2.5 parts of sweetness regulator, and 0.3-0.5 parts of stabilizer. The preparation method of the microencapsulated lavender oil powder is as follows: Step S1, dissolving casein peptide and soybean lecithin in deionized water, adjusting the pH to 6.0-7.0, adding transglutaminase, and cross-linking at 40-50° C. for 30-60 minutes to obtain a shell material solution; Step S2, adding lavender essential oil to the shell material solution, homogenizing under high pressure, and obtaining microencapsulated lavender oil powder through post-processing; The preparation method of the synergistic microcapsule powder is as follows: Step a, dissolving chitosan in acetic acid solution to form a chitosan solution, dissolving carboxymethyl cellulose in deionized water, then adding the chitosan solution, and mixing uniformly to obtain a shell material solution; Step b, mixing the theanine solution, 5-hydroxytryptophan solution, and piperine solution, and ultrasonicating for 10-20 minutes to obtain a core material solution; Step c: mixing the shell material solution and the core material solution, adjusting the pH to 4.5-5.0, stirring at room temperature for 20-40 minutes, adding tannic acid solution, stirring at room temperature for 4-5 hours, and obtaining synergistic microcapsule powder through post-treatment.

2. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The mass ratio of the casein peptide to soybean lecithin in step S1 is 1:1.5-2.5, the amount of casein peptide added to deionized water is 0.02-0.03 g / mL, and the amount of transglutaminase added per gram of casein peptide is 9-10 U / g.

3. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The mass volume ratio of the lavender essential oil to the shell material solution in step S2 is 0.01-0.025 g / mL.

4. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The concentration of the chitosan solution in step a is 1-3%, the amount of carboxymethyl cellulose added to deionized water is 1-1.5 g / 100 ml, and the mass ratio of chitosan to carboxymethyl cellulose is 2-2.5:

1.

5. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The mass concentration of theanine solution in step b is 0.1-0.2 g / 100 mL, the mass concentration of 5-hydroxytryptophan solution is 0.05-0.1 g / 100 mL, the mass concentration of piperine solution is 0.01-0.02 g / 100 mL, and the mass ratio of theanine, 5-hydroxytryptophan and piperine is 4-6:2-3:

1.

6. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: In step c, the volume ratio of the shell material solution to the core material solution is 1:3-4, and the mass volume ratio of tannic acid to the shell material solution is 0.0075-0.015 / 100 mL.

7. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The sweetness regulator is a mixture of erythritol and mogroside.

8. The sleep-aiding pitaya fermented liquid complex according to claim 1, characterized in that: The stabilizer is a mixture of high-ester pectin and propylene glycol alginate.

9. The method for preparing the sleep-aiding pitaya fermented liquid complex according to any one of claims 1 to 8, wherein: The following steps are involved: (1) Mixing the fermented pitaya liquid, microencapsulated lavender oil powder, synergistic microcapsule powder, sweetness regulator, and stabilizer to obtain a mixture; (2) The mixture obtained in step (1) is homogenized, and then filled, sterilized, and cooled.