Manufacturing method and device for long-term preservation of whole section specimen of rice soil

Abstract

The invention discloses a manufacturing method and device for long-term preservation of a rice soil whole section profile specimen, and belongs to the technical field of soil specimen manufacturing, and the method comprises the following steps: S1, sample collection: selecting a position with a clear soil layer and compact surface soil in a rice field to excavate a collection foundation pit, and repairing geometric sample soil on the pit wall of the collection foundation pit, in the repairing process, a thin sheet is used for inserting a plurality of inserting grooves in the sample soil, a sample box is used for stripping the sample soil from the foundation pit, and an original rice soil sample is obtained; s2, sample soil surface treatment: calculating the thickness of the required sample soil, removing soil blocks with redundant thickness by adopting a stainless steel knife, controlling the thickness of the trimmed sample soil to be 2-3cm greater than the calculated thickness, keeping each surface of the sample soil flat, avoiding pits, and storing at constant temperature and humidity; the method is used for solving the problems of short preservation time, easiness in weathering and loosening and difficulty in keeping a soil profile structure during preparation of an existing rice soil specimen.

Description

Technical Field

[0001] The invention belongs to the technical field of soil specimen preparation, and in particular relates to a preparation method and device for long-term preservation of a whole section specimen of paddy soil. Background Art

[0002] Paddy soil, a key factor in determining rice growth and yield, exhibits distinct stratification from the surface downward. Soil sampling is an essential step in conducting in-depth research on paddy soils.

[0003] Given that rice soils are rich in water and organic matter, the traditional method for preparing specimens is to collect soil samples in the field, allow them to air-dry to remove moisture, and then analyze them. However, over time, this method has exposed numerous drawbacks, such as soil weathering, loosening of the soil structure, and even the growth of other organisms. This results in extremely short sample storage times and difficulty maintaining the integrity of the soil profile, seriously compromising the accuracy and reliability of research. Therefore, this study proposed a specimen preparation method capable of long-term preservation of rice soil profiles and designed an auxiliary tool to improve the efficiency and quality of specimen preparation. Summary of the Invention

[0004] In response to the above problems, the present invention provides a method and device for long-term preservation of whole-section cross-section specimens of paddy soil, which is used to solve the problems of short storage time, easy weathering and looseness during the preparation of existing paddy soil specimens, and difficulty in maintaining the soil cross-section structure.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] A method for long-term preservation of a whole section of paddy soil specimen comprises the following steps:

[0007] S1 Sample Collection: A sampling pit was excavated at a location in the rice field with a clear soil layer and compacted surface soil. A geometric sample of soil was cut out from the pit wall. During the repair process, a thin sheet was used to insert several slots into the sample soil. The sample soil was then peeled off from the pit using a sample box to obtain the original rice soil sample.

[0008] S2 Sample soil surface treatment: Calculate the required sample soil thickness, use a stainless steel knife to remove excess soil clods, control the trimmed sample soil thickness to be 2-3 cm greater than the calculated thickness, and keep the sample soil surface flat to avoid pits, and store it under constant temperature and humidity;

[0009] S3 Moisture Control: Allow the soil to dry naturally, keeping the moisture content of the top 3-5cm of the planting area at 20%-25%, and the moisture content of the top 1-2cm of the remaining area at 18%-20%;

[0010] S4 irradiation disinfection: Place the rice soil samples that have been dried to sterilize them in an irradiation workshop, maintaining a minimum irradiation dose of 10 kGy.

[0011] S5 Gluing and curing: Use AB glue to seal the disinfected soil sample; Two coats of glue are used in total. The fluidity of the first coat is greater than that of the second coat, and the first coat is left to stand for at least 18 hours and cured for at least 48 hours. The second coat of glue is applied after the first coat is completely cured, and the glue is left to stand for at least 10 hours and cured for at least 48 hours. The gluing process should be carried out slowly to avoid damage to the soil caused by the impact of the glue. After curing, the excess glue is trimmed and polished to facilitate observation.

[0012] S6 Sealed Storage: Place the solidified and trimmed soil sample in a dedicated sample storage container and store it in vacuum.

[0013] As a further improvement to the above solution, in step S2, the thickness of the sample soil is calculated according to the radiation sterilization dose and the minimum specimen thickness, and the calculation formula is as follows: , and x>minimum specimen thickness;

[0014] Where x represents the soil thickness, c is a constant related to the irradiation source, t is the irradiation time, and D is the target dose.

[0015] As a further improvement of the above solution, in step S3, in order to prevent the soil surface from cracking due to excessive drying, water mist is sprayed on the soil surface until the moisture control requirement of the sample soil is met.

[0016] Compared with the existing technology, the beneficial effects of the present invention are as follows: in the process of preparing soil samples, unlike traditional sample preparation methods, by irradiating and sterilizing the soil, bacteria and microorganisms in the soil can be effectively killed, the original characteristics of the soil can be maintained, and the damage to the soil samples by organisms and microorganisms in the soil can be avoided, thereby achieving the effect of long-term preservation; and after irradiation and sterilization, glue is used for curing, which can achieve sealed preservation of the soil samples. In particular, after the glue is cured, the structure of the soil can be significantly fixed, the soil can be prevented from dispersing, and the stability of the soil structure can be maintained, which is convenient for research and use.

[0017] In addition, a device for long-term preservation of whole-section cross-section specimens of paddy soil is provided, which is used to implement the gluing and curing steps in the specimen preparation method, comprising a first holding box and a second holding box. The sample soil is placed in the second holding box to complete the first gluing, and the second holding box is detachably buckled into the first holding box to complete the second gluing.

[0018] The first storage box can be flipped, and a lifting assembly connected to the interior of the first storage box is arranged on the side of the first storage box. A support member that can lift the bottom of the inverted second storage box is arranged on the top lifting rod of the lifting assembly.

[0019] The technical effect of the above improvement is: during the first gluing process, firstly, glue with good fluidity is injected into the second holding box, and the glue will quickly wrap the sample soil, so that a layer of gel coat can be formed on the surface of the sample soil first. The purpose of the gel coat is mainly to simply seal the sample soil to prevent the soil from dispersing and cracking, which is conducive to maintaining the soil structure. After the first gluing is completed, the first holding box is flipped down, and then the second holding box is installed in the first holding box. The first holding box is flipped up again, and the second holding box and the sample soil are buckled in the first holding box. At this time, by injecting glue into the first holding box for the second time, the second glue can be protected by the first glue to avoid damage to the soil. Therefore, the two gluings can improve the integrity of the sample soil during gluing and preservation.

[0020] As a further improvement to the above solution, a bottom plate is movably provided in the second storage box, and a delivery pipe for delivering glue is fixedly provided at the bottom edge of the second storage box, and an overflow port is provided on the delivery pipe to facilitate the glue to evenly overflow outward;

[0021] A protruding support platform is provided on the bottom plate, and a plurality of overflow holes are provided on the edge of the bottom plate. The feed pipe is surrounded by the bottom plate, the support platform and the second storage box, and the glue transported on the feed pipe passes through the overflow holes and enters the second storage box.

[0022] The technical effect of the above improvement is that the first layer of glue can be evenly injected into the second holding box through the provided feed pipe, and with the support of the bottom plate, the glue gradually wraps the sample soil upward through the overflow hole, thereby preventing the impact of the glue flowing out of the feed pipe from affecting the integrity of the soil.

[0023] As a further improvement to the above solution, the bottom of the first holding box is connected to a plurality of glue injection ports for injecting glue into the first holding box. A support plate is provided in the first holding box to support the inverted soil sample on the second holding box that has been glued for the first time.

[0024] The feed port of the lifting assembly is connected to a connecting pipe that is interconnected with the first containing box, and a solenoid valve is provided on the pipe of the discharge port. The solenoid valve and the glue injection port are respectively connected to a pipeline system for controlling the flow of glue.

[0025] The technical effect of the above improvement is: through the set solenoid valve, the glue injected into the first holding box can be synchronously entered into the lifting assembly. Therefore, during the continuous glue injection process, the lifting rod of the lifting assembly can be synchronously pushed upward, and the second holding box can also be synchronously driven to lift upward, so as to facilitate the sample soil in the second holding box to be slowly exposed, so that continuous pressure support can be provided on the edge of the soil to prevent the soil from breaking.

[0026] As a further improvement to the above solution, a sealing cover plate is detachably provided on the top surface of the first storage box, the sealing cover plate and the second storage box are movably sealed with each other, and the sealing cover plate and the support plate are fixed to each other by a connecting rod;

[0027] There is a channel between the support plate and the inner wall of the first containing box to facilitate the flow of glue, and the support plate is also provided with a number of fine holes running through it from top to bottom. The side of the support plate is provided with an elastic valve in contact with the inner wall of the first containing box, and a heating component is also provided inside the support plate.

[0028] The technical effect of the above improvement is: under the support of the sealing cover and the connecting rod, the support plate can be kept in the first holding box without blocking the glue injection port, so that the glue injected into the glue injection port can smoothly enter the first holding box. The heating component will heat the glue at the beginning of the glue injection process, thereby accelerating the solidification and condensation of the glue on the support plate, so that a support area with relatively high hardness is formed on the support plate, which can effectively support the sample soil above. Moreover, as time goes by, the hardness of the glue supported above the support plate becomes stronger and stronger, and the supporting effect becomes better and better. When the glue in the first holding box completely wraps the sample soil, the glue injection is stopped, and the glue in the first holding box and the lifting component is reversely drawn back through the pipeline system. During the withdrawal process, due to the one-way sealing effect of the elastic valve, the glue above the support plate can be prevented from being sucked away, so the glue above the support plate can be maintained until it is completely solidified.

[0029] As a further improvement to the above solution, the lifting assembly and the first receiving box are respectively fixedly arranged on a base, a bottom frame is provided below the base, a turning space is provided on the bottom frame, the base and the bottom frame are rotatably arranged relative to each other, and the base can be turned around an axis within the turning space;

[0030] The base frame is also provided with a locking assembly that supports the position of the locking base. The locking assembly includes locking plates that are slidably arranged on the top surface of the base frame and are symmetrically arranged. When the locking plates move toward the middle, they contact and support the base ground.

[0031] The technical effect of the above improvement is that the symmetrical arrangement of the locking plates allows them to move synchronously toward the middle after the first and second storage boxes are flipped upwards, so as to support the lower support column of the base, thereby fixing the first and second storage boxes.

[0032] As a further improvement to the above solution, a support arm is fixedly provided on the top of the locking plate, and a clamping seat is slidably provided on the horizontal arm of the support arm;

[0033] The second receiving box is provided with a connecting port which passes through the connecting port, and the bottom plate is detachably provided with a supporting column which can pass through the connecting port and be clamped and fixed with the clamping seat.

[0034] As a further improvement of the above solution, a connecting platform is provided on the second storage box, the connecting port is located in the middle of the connecting platform, and a connecting socket is provided on the side of the connecting platform. A fixing hook that can be inserted into the connecting socket is movably provided on the horizontal arm of the support arm.

[0035] The technical effect of the above improvement is as follows: by providing the support column, the bottom plate can be supported during the second glue injection process, so that when the second holding box moves upward, the bottom plate is supported and does not move, so that the sample soil in the second holding box can be evenly pushed out of the second holding box for the second glue injection. When the second holding box moves to the uppermost position, since excess glue needs to be drawn back, in order to prevent the second holding box from falling, the fixing hook can be inserted into the connecting jack to hook and fix the second holding box. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the overall structure of the device of the present invention;

[0037] Figure 2 for Figure 1 Schematic diagram of the structure in the A direction;

[0038] Figure 3 for Figure 1 Schematic diagram of the local enlarged structure at B in the middle;

[0039] Figure 4 for Figure 1 Schematic diagram of the local enlarged structure at C in the middle;

[0040] Figure 5 for Figure 1 Schematic diagram of the local enlarged structure at D in the middle;

[0041] Figure 6 Schematic diagram of the structure inside the second containing box;

[0042] Figure 7 for Figure 6 Schematic diagram of the local enlarged structure at E in the middle;

[0043] Figure 8 Schematic diagram of the state during the second gluing process;

[0044] Figure 9 for Figure 8 Schematic diagram of the local enlarged structure at F in the middle;

[0045] Figure 10 Schematic diagram of the structure of the feed pipe;

[0046] Figure 11 This is a simple schematic diagram of the piping system;

[0047] Figure 12 Schematic diagram of the structure of the collected paddy soil samples;

[0048] Figure 13 Schematic diagram of the simplified process of gluing.

[0049] In the figure: 10. First containing box; 11. Second containing box; 111. Bottom plate; 112. Support platform; 113. Overflow hole; 114. Feed pipe; 1141. Overflow port; 115. Connecting port; 116. Connecting platform; 117. Connecting socket; 12. Lifting assembly; 13. Support member; 14. Connecting pipe; 15. Solenoid valve; 16. Glue injection port; 17. Support plate; 171. Heating assembly; 18. Base; 19. Sealing cover; 20. Connecting rod; 21. Elastic valve; 22. Base frame; 221. Turning space; 23. Locking assembly; 231. Locking plate; 24. Support arm; 25. Clamping seat; 26. Support column; 27. Fixing hook; 30. First pump body; 31. First valve body; 32. Second valve body; 33. Second pump body. DETAILED DESCRIPTION

[0050] In order to enable those skilled in the art to better understand the technical solution, the present invention is described in detail below in conjunction with embodiments. The description in this section is only exemplary and explanatory and should not have any limiting effect on the scope of protection of the present invention.

[0051] The specific solution of this embodiment is: a method for preparing a long-term preservation of a whole section of paddy soil specimen, comprising the following steps:

[0052] S1 Sample collection: A collection pit is excavated at a location in the rice field with a clear soil layer and compacted surface soil. The size of the excavated collection pit is generally a 1m×1m×1m cubic pit, and a geometric sample soil is repaired on the pit wall of the collection pit, generally in the shape of a rectangular parallelepiped. During the repair process, a thin sheet is used to insert several slots in the sample soil. Specifically, the slot is a gap that can be inserted by a blade, and a cross-shaped slot can be used. The number of slots should not be too many, 3-5. The slots are mainly set to facilitate the evaporation of water and to facilitate the entry into the gap during the gluing process to improve the curing strength. Then, a sample box is used to peel the sample soil from the pit to obtain the original rice soil sample;

[0053] S2 Sample soil surface treatment: Calculate the required sample soil thickness, use a stainless steel knife to remove excess soil clods, and control the thickness of the trimmed sample soil to be 2-3 cm greater than the calculated thickness. This is mainly to reserve a margin for water evaporation, but also to maintain the strength of the soil and avoid breakage. The surface of the sample soil should be kept flat to avoid pits and stored under constant temperature and humidity.

[0054] S3 Moisture Control: Allow to dry naturally, keeping the moisture content of the top 3-5cm layer of the planting area at 20%-25%, and the moisture content of the top 1-2cm layer of the remaining area at 18%-20%. The planting area is the deepest part that the rice roots can reach during the planting process, and the rest of the area is basically the deep soil below the planting area. Please refer to the attached Figure 12 In the actual samples, the boundary between the planted part and the deep soil is not obvious. The boundary in the attached figure is only for reference and does not represent the actual boundary;

[0055] S4 irradiation disinfection: Place the rice soil samples that have been dried to sterilize them in an irradiation workshop, maintaining a minimum irradiation dose of 10 kGy.

[0056] S5 gluing and curing; AB glue is used to seal the disinfected soil sample; two coats of gluing are used in total, the fluidity of the first coat is greater than that of the second coat, and the first coat is left to stand for at least 18 hours after application and cured for at least 48 hours. The first coat of gluing is mainly to fix the structure of the soil to avoid soil breakage, falling off, or peeling of some soil; the second coat of gluing is applied after the first coat is completely cured, and the coat is left to stand for at least 10 hours after application and cured for at least 48 hours. The gluing process should be carried out slowly to avoid damage to the soil due to the impact of the glue. After curing, the excess glue is trimmed and polished to facilitate observation;

[0057] S6 Sealed Storage: Place the solidified and trimmed soil sample in a dedicated sample storage container and store it in vacuum.

[0058] As a preferred embodiment of the above, in step S2, the thickness of the sample soil is calculated according to the dose of irradiation disinfection and the minimum thickness of the specimen, and the calculation formula is as follows: , and x>minimum specimen thickness;

[0059] Wherein, x represents the soil thickness, c is a constant related to the irradiation source, t is the irradiation time, and D represents the target dose. For the specific derivation, please refer to patent CN112121188B.

[0060] As a preferred embodiment of the above embodiment, in step S3, in order to prevent the soil surface from cracking after being too dry, water mist is sprayed on the soil surface until the moisture control requirements of the sample soil are met. The water mist is sprayed mainly to control the moisture of the surface soil to avoid the surface soil from cracking and breaking after water loss. This is mainly because the planting part of paddy soil is more sticky than ordinary soil, and the moisture in the outer layer of the soil evaporates easily, but the moisture in the inner layer of the soil evaporates more difficultly. Therefore, it is difficult to ensure that the moisture inside and outside are consistent. By extending the water control time and continuously replenishing water to the surface soil, it is also to preserve the complete cross-sectional structure of the soil while keeping the moisture inside the soil up to standard, so as to facilitate dispersion and cracking in the later injection process.

[0061] In order to maintain the integrity of the soil structure during the process of soil glue curing and avoid the soil from splitting and dispersing during the glue curing process, the present application also provides a preparation device for long-term preservation of the entire section specimen of paddy soil, which is used to implement the glue curing step in the preparation method provided by the present application. For details, please refer to the attached Figure 1-11 13. The structure of the device includes a first holding box 10 and a second holding box 11. The sample soil is placed in the second holding box 11 to complete the first gluing. The second holding box 11 can be detachably buckled into the first holding box 10 to complete the second gluing.

[0062] The first storage box 10 can be turned over, and a lifting assembly 12 is provided on the side of the first storage box 10 and is connected to the interior of the first storage box 10. A support member 13 that can lift the bottom of the inverted second storage box 11 is provided on the top lifting rod of the lifting assembly 12. For details, please refer to the attached Figure 1As shown, the lifting assembly 12 and the first holding box 10 are respectively fixedly arranged on the base 18, and the base 18 includes a supporting plate at the top and a supporting square steel at the bottom of the supporting plate. A base frame 22 is provided below the base 18, and a turning space 221 is provided on the base frame 22. The turning space 221 is specifically a through-hole groove that is completely penetrated from top to bottom and is provided on the base frame 22. The base 18 and the base frame 22 are rotatably arranged relative to each other, and the base 18 can be turned around the axis in the turning space 221. The rotation axis between the base 18 and the base frame 22 is set at the lower surface position of the platform plate at the top of the base frame 22; during the second gluing process, in order to facilitate the transfer of the sample after the first gluing on the second holding box 11 to the first holding box 10, the base 18 is first turned over to the position where the opening of the first holding box 10 is facing downward, and then the sample is buckled in the first holding box 10. After the base 18 is turned over again, the opening of the first holding box 10 is facing upward, so that the soil sample inside can be supported when the sample is transferred to avoid damage;

[0063] The base frame 22 is also provided with a locking assembly 23 for supporting the position of the locking base 18. The locking assembly 23 includes a locking plate 231 which is slidably arranged on the top surface of the base frame 22 and is symmetrically arranged. When the locking plate 231 moves toward the middle, it contacts the ground of the base 18 for support. Figure 2 As shown, gear racks are used between the symmetrical locking plates 231 to achieve synchronous movement of the two locking plates 231, that is, when the first storage box 10 is opened upward and the second gluing process is normally carried out, by pulling the locking plate 231 to move toward the middle, the locking plate 231 can synchronously support the bottom of the base 18, thereby avoiding the possibility of the base 18 flipping to both sides. In the process of transferring samples, the locking plate 231 is synchronously pulled to both sides to open the flipping space 221, and the first storage box 10 can be normally flipped downward to the position where the opening faces downward.

[0064] like Figure 6 、 7 As shown in FIG10 , as a preferred embodiment of the above embodiment, a bottom plate 111 is movably provided in the second holding box 11, and the bottom plate 111 supports the sample soil. A delivery pipe 114 for delivering glue is fixedly provided at the bottom edge of the second holding box 11, and an overflow port 1141 is provided on the delivery pipe 114 to facilitate the glue to evenly overflow outward. Figure 10 The overflow port 1141 is a spiral notch provided on the delivery pipe 114, which allows the injected glue to overflow evenly;

[0065] The bottom plate 111 is provided with a protruding support platform 112 for attaching Figure 6 、 7Taking the up and down direction as an example, the support platform 112 is arranged on the lower side of the bottom plate 111 and contacts the bottom wall of the second storage box 11. The support platform 112 is a ring structure, close to the edge of the bottom plate 111, mainly to lift the bottom plate 111 upward by a certain height. The edge of the bottom plate 111 is provided with a plurality of overflow holes 113 running through the top and bottom. The feeding pipe 114 is surrounded by the bottom plate 111, the support platform 112 and the second storage box 11, and the glue transported on the feeding pipe 114 passes through the overflow hole 113 into the second storage box 11. In addition, in order to allow the glue delivered from the feeding pipe 114 to spread on the bottom plate 111 and fill the gap between the bottom plate 111 and the sample soil, a large number of fine grooves are provided on the top surface of the bottom plate 111. The fine grooves can guide the injected glue so that the glue can also cover the ground of the sample soil. Moreover, in order to reduce the transfer process of the sample soil from the sampling step to the gluing step, the second holding box 11 can be directly used as a sampling frame in the sampling process, and can also be directly used as a storage container in the irradiation sterilization process.

[0066] like Figure 4 、 5 As shown in Figures 8 and 11, as a preferred embodiment of the above embodiment, the bottom of the first holding box 10 is connected to a plurality of glue injection ports 16 for injecting glue into the first holding box 10. A support plate 17 is provided in the first holding box 10 to support the inverted soil sample on the second holding box 11 that has been glued for the first time. This can reduce the direct impact caused by the glue injection and prevent the sample soil from being crushed.

[0067] The feed port of the lifting assembly 12 is connected to a connecting pipe 14 that is connected to the first receiving box 10. Specifically, in this embodiment, the lifting assembly 12 is a piston-type lifting cylinder, and a solenoid valve 15 is provided on the pipe of the discharge port. The solenoid valve 15 and the glue injection port 16 are respectively connected to a pipeline system for controlling the flow of glue. The pipeline system is as follows: Figure 11As shown, the specific working process is as follows; during the second glue injection process, the solenoid valve 15 and the first valve body 31 are first opened, and the second valve body 32 is closed. By starting the first pump body 30, glue is injected into the first receiving box 10. Since the first receiving box 10 is pressurized by the second receiving box 11, the glue in the first receiving box 10 will enter the lifting assembly 12 through the connecting pipe 14, and flow out through the second pump body 33 after passing through the solenoid valve 15. Then, by closing the solenoid valve 15, the pressure in the lifting assembly 12 and the first receiving box 10 will accumulate, and the glue will continuously enter the first receiving box 10 and the lifting assembly 12, so that in the process of the lifting assembly 12 pushing the first receiving box 10 to rise, the second glue injection operation is carried out synchronously. After the glue injection is completed, the first pump body 30 is stopped, the first valve body 31 is closed, the solenoid valve 15 and the second valve body 32 are opened, and the second pump body 33 is started, so that the excess glue in the first receiving box 10 and the lifting assembly 12 can be sucked out.

[0068] A sealing cover 19 is detachably provided on the top surface of the first holding box 10. The sealing cover 19 is annular and is movably sealed with the second holding box 11. Specifically, a sealing ring is provided on the sealing cover 19 to seal with the second holding box 11. The sealing cover 19 and the support plate 17 are fixed to each other through a connecting rod 20. The connecting rod 20 is a connecting rod provided, so that the support plate 17 can be suspended in the second holding box 11, thereby avoiding the support plate 17 from obstructing the glue injection port 16 and also supporting and protecting the sample soil. Figure 4 、 5 As shown; there is a channel between the support plate 17 and the inner wall of the first storage box 10 for facilitating the flow of glue, and the channel is specifically the interval between the support plate 17 and the inner wall of the first storage box 10, and the support plate 17 is also provided with a plurality of fine holes running through it from top to bottom, and the side of the support plate 17 is provided with an elastic valve 21 in contact with the inner wall of the first storage box 10, and the elastic valve 21 is specifically annular and fixed at the edge of the support plate 17, as shown Figure 4As shown, the elastic valve 21 is made of rubber material and has a one-way flow restriction function that only allows glue to flow upward. A heating component 171 is also provided in the support plate 17. The heating component 171 is specifically an electric heating plate. During the glue injection process, the heating of the heating component 171 can increase the solidification speed of the glue around the support plate 17, thereby increasing the hardness of the glue. Especially when the glue is just being injected, the glue on the support plate 17 can be allowed to solidify to a certain hardness before continuous glue injection. When the sample soil in the second holding box 11 is separated from the second holding box 11, it will better support the sample soil and avoid direct contact with the support plate 17. In the sample after the final molding, the second glue will completely wrap and seal the sample soil, so that the glue application of the sample has a better sealing effect.

[0069] In addition, in this embodiment, in order to ensure the normal circulation of glue, some valves for maintaining air pressure balance are provided on the first containing box 10 and in the piping system, but they are not shown in the drawings and do not affect the operation of the system.

[0070] like Figure 1 、 2 , 3, 8, and 9, as a preferred embodiment of the above, a support arm 24 is fixedly provided on the top of the locking plate 231, and a clamping seat 25 is slidably provided on the horizontal arm of the support arm 24, and the clamping seat 25 is "C"-shaped;

[0071] The second container 11 is provided with a connecting port 115, and the bottom plate 111 is detachably provided with a supporting column 26 which can pass through the connecting port 115 and be fixed to the clamping seat 25. For details, please refer to the attached Figure 3 As shown, the support column 26 and the second receiving box 11 are fixed by threads, and the support column 26 is provided with a plurality of grooves that engage with the clamping seat 25; the second receiving box 11 is provided with a connecting platform 116, and the connecting port 115 is located in the middle position of the connecting platform 116. The connecting platform 116 is specifically located at the bottom of the second receiving box 11, and the side of the connecting platform 116 is provided with a connecting socket 117. A fixing hook 27 that can be inserted into the connecting socket 117 is movably provided on the cross arm of the support arm 24. Specifically, during the working process, the second gluing will be pushed upward to the top position by the lifting assembly 12. In order to prevent the second receiving box 11 from falling after the excess glue is drawn back, the second receiving box 11 can be hooked by the fixing hook 27 to fix the position of the second receiving box 11, thereby avoiding affecting the curing process of the second gluing.

[0072] The working principle of the device is as follows: first, when sampling soil, the sample soil is placed in the second holding box 11. After processing, during the first gluing process, the first layer of glue can be introduced into the second holding box 11 by connecting the feed pipe 114 to the glue injection pipe. The glue can completely cover the sample soil, thus completing the first gluing process;

[0073] After the first glue is completely cured, the base 18 and the first receiving box 10 are flipped downward so that the opening of the first receiving box 10 is downward, and then the second receiving box 11 is buckled into the first receiving box 10. At the same time, the first receiving box 10 is flipped upward, and the locking plate 231 is pushed toward the middle to fix the position of the base 18. Then, a second layer of glue is injected into the second receiving box 11 through the pipeline system. During the process of injecting glue, the second receiving box 11 is driven to rise upward under the action of the lifting component 12, so that the sample soil is gradually glued for the second time in the second receiving box 11. After the gluing is completed, the pipeline system is controlled again to extract the excess glue, and then the glue in the second receiving box 11 is kept completely cured. After the curing is completed, it is taken out of the second receiving box 11 and separated from the support plate 17. After trimming, the cured sample is obtained. During the two gluing processes of the sample soil by this device, the change process of the sample soil is as follows Figure 13 shown.

[0074] It should be noted that, in this article, the terms include, comprise or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. Specific examples are used herein to illustrate the principles and implementation methods of the technical solution of the present invention. The above examples are only used to help understand the method of the present invention and its core idea. The above is only a preferred embodiment of the present invention. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should all be regarded as the scope of protection of the present invention.

Claims

1. A method for long-term preservation of whole-section cross-section specimens of paddy soil, characterized in that: The following steps are involved: S1 Sample Collection: A sampling pit was excavated at a location in the rice field with a clear soil layer and compacted surface soil. A geometric sample of soil was cut out from the pit wall. During the repair process, a thin sheet was used to insert several slots into the sample soil. The sample soil was then peeled off from the pit using a sample box to obtain the original rice soil sample. S2 Sample soil surface treatment: Calculate the required sample soil thickness, use a stainless steel knife to remove excess soil clods, control the trimmed sample soil thickness to be 2-3 cm greater than the calculated thickness, and keep the sample soil surface flat to avoid pits, and store it under constant temperature and humidity; S3 Moisture Control: Allow the soil to dry naturally, keeping the moisture content of the top 3-5cm of the planting area at 20%-25%, and the moisture content of the top 1-2cm of the remaining area at 18%-20%; S4 irradiation disinfection: Place the rice soil specimens that have been dried to a minimum of 10 kGy in an irradiation room for disinfection. S5 Gluing and curing; Use AB glue to seal the disinfected soil sample; Two coats of glue are used in total. The fluidity of the first coat is greater than that of the second coat, and the first coat is left to stand for at least 18 hours and cured for at least 48 hours. The second coat of glue is applied after the first coat is completely cured, and the coat is left to stand for at least 10 hours and cured for at least 48 hours. During the gluing process, avoid damaging the soil due to impact of the glue. After curing, trim the excess glue and polish it to make it smooth for observation. S6 Sealed Storage: Place the solidified and trimmed soil sample in a dedicated sample storage container and store it in vacuum.

2. The method for long-term preservation of a whole section of paddy soil according to claim 1, characterized in that: In step S2, the thickness of the sample soil is calculated according to the radiation sterilization dose and the minimum thickness of the specimen. The calculation formula is as follows: , and x>minimum specimen thickness; Where x represents the soil thickness, c is a constant related to the irradiation source, t is the irradiation time, and D is the target dose.

3. The method for long-term preservation of a whole section of paddy soil according to claim 1, characterized in that: In step S3, in order to prevent the soil surface from cracking due to excessive drying, water mist is sprayed on the soil surface until the moisture control requirement of the sample soil is met.

4. A device for long-term preservation of whole-section cross-section specimens of paddy soil, used to implement the gluing and curing step in the method according to any one of claims 1 to 3, characterized in that: The invention comprises a first containing box (10) and a second containing box (11), wherein the sample soil is placed in the second containing box (11) to complete the first gluing, and the second containing box (11) is detachably buckled in the first containing box (10) to complete the second gluing; The first storage box (10) is flippable, and a lifting assembly (12) connected to the interior of the first storage box (10) is provided on the side of the first storage box (10), and a support member (13) capable of lifting the bottom of the inverted second storage box (11) is provided on the top lifting rod of the lifting assembly (12).

5. The device according to claim 4, characterized in that A bottom plate (111) is movably provided in the second containing box (11), a delivery pipe (114) for delivering glue is fixedly provided at the bottom edge of the second containing box (11), and an overflow port (1141) is provided on the delivery pipe (114) for facilitating the glue to evenly overflow outwards; A protruding support platform (112) is provided on the bottom plate (111), and a plurality of overflow holes (113) extending vertically through the edge of the bottom plate (111) are provided. The feed pipe (114) is surrounded by the bottom plate (111), the support platform (112), and the second storage box (11), and the glue transported on the feed pipe (114) passes through the overflow holes (113) and enters the second storage box (11).

6. The device according to claim 4, characterized in that The bottom of the first containing box (10) is connected to a plurality of glue injection ports (16) for injecting glue into the first containing box (10), and a support plate (17) is provided in the first containing box (10), and the support plate (17) supports the inverted soil sample on the second containing box (11) that has been glued for the first time; The feed port of the lifting assembly (12) is connected to a connecting pipe (14) that is interconnected with the first containing box (10), and a solenoid valve (15) is provided on the pipe of the discharge port. The solenoid valve (15) and the glue injection port (16) are respectively connected to a pipeline system for controlling the flow of glue.

7. The device according to claim 6, characterized in that A sealing cover plate (19) is detachably provided on the top surface of the first receiving box (10), the sealing cover plate (19) and the second receiving box (11) are movably sealed to each other, and the sealing cover plate (19) and the support plate (17) are fixed to each other via a connecting rod (20); A channel for facilitating the flow of glue is provided between the support plate (17) and the inner wall of the first receiving box (10), and a plurality of fine holes extending upward and downward are provided on the support plate (17). An elastic valve (21) in contact with the inner wall of the first receiving box (10) is provided on the side of the support plate (17), and a heating component (171) is also provided in the support plate (17).

8. The device according to claim 5, characterized in that The lifting assembly (12) and the first receiving box (10) are respectively fixedly arranged on the base (18); a bottom frame (22) is arranged below the base (18); a turning space (221) is arranged on the bottom frame (22); the base (18) and the bottom frame (22) are rotatably arranged relative to each other, and the base (18) can be turned around an axis in the turning space (221); The base frame (22) is also provided with a locking assembly (23) for supporting the position of the locking base (18). The locking assembly (23) includes a locking plate (231) slidably arranged on the top surface of the base frame (22) and arranged symmetrically. When the locking plate (231) moves toward the middle, it contacts and supports the base (18) on the ground.

9. The device according to claim 8, characterized in that A support arm (24) is fixedly provided on the top of the locking plate (231), and a clamping seat (25) is slidably provided on the horizontal arm of the support arm (24); The second receiving box (11) is provided with a connecting port (115) that penetrates through the connecting port (115), and the bottom plate (111) is detachably provided with a supporting column (26) that can pass through the connecting port (115) and be clamped and fixed to the clamping seat (25).

10. The device according to claim 9, characterized in that A connecting platform (116) is provided on the second receiving box (11), a connecting port (115) is located in the middle of the connecting platform (116), a connecting socket (117) is provided on the side of the connecting platform (116), and a fixing hook (27) that can be inserted into the connecting socket (117) is movably provided on the cross arm of the support arm (24).

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