Culture equipment for biological cell tissues

Abstract

The invention discloses culture equipment for biological cell tissues, and relates to the technical field of biological cell culture. The device comprises a culture box and a rotating shaft, the rotating shaft is rotatably installed in the culture box, a box door is fixedly installed on the rotating shaft, a transmission mechanism is arranged on the rotating shaft, the transmission mechanism is meshed with a rack, a connecting rod is fixedly installed on the rack, the connecting rod is tightly attached to an air rod, the air rod is slidably connected into an air cylinder, and an air guide pipe is arranged at the front end of the air cylinder. A one-way control valve is installed at the front end of the air guide pipe, the front end of the air guide pipe is communicated with a disinfection water tank, the front side of the disinfection water tank is communicated with a pressure valve pipe, the lower end of the pressure valve pipe is communicated with a splitter plate, and a rotating shaft is arranged in the incubator. By opening the box door, the splitter plate can automatically spray vaporific disinfectant fluid downwards, and the vaporific disinfectant fluid can form an atomized protective layer of the disinfectant fluid, so that bacteria or impurities in the external environment are prevented from entering the incubator after the box door is opened.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological cell culture, and in particular relates to a culture device for biological cell tissues. Background Art

[0002] Biological cell and tissue culture equipment refers to various instruments and tools used to culture and observe cell tissues in vitro. These devices have a wide range of applications in fields such as cell biology, biomedicine, and drug discovery. They help scientists and researchers gain a deeper understanding of the mechanisms of cell growth, development, and disease progression, and can also be used in therapeutics and drug development. Common biological cell and tissue culture equipment includes cell culture dishes, cell culture plates, microscopes, centrifuges, shakers, and microplate readers. These devices require strict sterile conditions to ensure that the cell growth environment is similar to that in vivo, and they require regular cleaning and maintenance to ensure accuracy and reliability.

[0003] After searching, Chinese patent number CN202310956296.3 discloses a biological stem cell culture method and equipment thereof, which relates to the technical field of cell culture equipment, including a culture box body, a sealed door with a door lock hingedly provided on the culture box body, an air pump installed on the top of the culture box body, and the air pump is connected to the culture box body through a hose. In the present invention, when in use, the culture plate is first placed in the culture box body, and when it is placed, the sealed door is closed. The rotation of the sealed door will cause the fixed plate to move upward. When the sealed door is completely closed, the fixed plate will abut against the horizontal block, and then multiple culture plates can be quickly fixed, thereby reducing the opening time of the sealed door, avoiding fixing one by one and causing the sealed door to be opened for a long time, which affects the culture of stem cells. When it is necessary to take the culture bottle, the sealed door is opened. The opening of the sealed door will cause the fixed plate to move downward, thereby releasing the fixation of the culture plate, making it easier for the user to take it.

[0004] However, when the above patent is in use, when the staff takes and places cells, they need to open the box door, which will cause bacteria or impurities in the external environment to enter the interior of the box. As a result, bacteria and impurities in the external environment continue to enter the interior of the box, which may cause contamination of the cells cultured therein; at the same time, the above patent is also unable to fix cell culture tubes of different sizes. Summary of the Invention

[0005] In response to the problems in the related art, the present invention proposes a culture device for biological cell tissue to overcome the above technical problems existing in the existing related art.

[0006] To solve the above technical problems, the present invention is achieved through the following technical solutions:

[0007] The present invention provides a culture device for biological cell tissues, comprising an incubator and a rotating shaft, wherein the rotating shaft is rotatably mounted inside the incubator, the rotating shaft is fixedly mounted with a box door, the rotating shaft is provided with a transmission mechanism, the transmission mechanism is meshed with a rack, the rack is fixedly mounted with a connecting rod, the connecting rod is tightly attached to the gas rod, the gas rod is slidably connected to the inside of the gas cylinder, an air guide tube is provided at the front end of the gas cylinder, a one-way control valve is installed at the front end of the air guide tube, the front end end of the air guide tube is connected to a disinfection water tank, the front side of the disinfection water tank is connected to a pressure valve pipe, the lower end of the pressure valve pipe is connected to a diverter plate, a rotating shaft is provided inside the incubator, one end of a rod is rotatably mounted on the rotating shaft, and a slider is rotatably mounted on the other end of the rod, the slider is slidably connected to the box door, the rotating shaft is provided with a fixed plate, and a test tube placement mechanism is provided inside the fixed plate.

[0008] Furthermore, the test tube placement mechanism includes a test tube placement box arranged on a fixed plate, the test tube placement box is fixedly mounted on the fixed plate, a disc is fixedly mounted inside the test tube placement box, a clamping plate is slidably connected above the disc, a protrusion is fixedly mounted above the clamping plate, the protrusion is slidably connected to the inside of an arc-shaped slide groove, the arc-shaped slide groove is opened on a rotating disk, and the rotating disk is rotatably mounted inside the test tube placement box.

[0009] Furthermore, a plurality of the clamping plates, protruding blocks and arc-shaped slide grooves are provided, and their parts and installation methods are the same.

[0010] Furthermore, the transmission mechanism includes a first gear arranged on the rotating shaft, the first gear is fixedly mounted on the first gear, the first gear is connected to the second gear through a gear belt transmission, and the second gear is meshed with a rack.

[0011] Furthermore, the rotating shaft is rotatably installed inside the incubator, the sliding block is slidably connected inside a chute, the chute is opened on the inner side of the box door, and the fixing plate is fixedly installed on the rotating shaft.

[0012] Furthermore, the air guide tube is connected to the front end of the air cylinder, the disinfection water tank is fixedly installed on the incubator, the diverter plate is fixedly installed on the incubator, and a nozzle is provided at the bottom of the diverter plate, and a plurality of nozzles are provided.

[0013] Furthermore, the box door is provided with a transparent observation window, and the box door and the incubator can be made of stainless steel.

[0014] Furthermore, a sealing strip is provided between the box door and the incubator.

[0015] The present invention has the following beneficial effects:

[0016] 1. The present invention opens the door, and the diverter plate automatically sprays disinfectant water in a mist form downwards. At this time, the disinfectant water in a mist form an atomized protective layer of disinfectant water, thereby reducing the bacteria or impurities in the external environment from entering the interior of the incubator after the door is opened.

[0017] 2. The present invention drives the slider to slide along the inner side of the door while the door is being opened, thereby driving the rod to rotate inside the incubator, thereby driving the fixed plate to rotate, so that the fixed plate is rotated to the outside of the incubator. At this time, the test tube placement mechanism can be disinfected by disinfectant mist, avoiding the need for the operator to reach into the incubator to take the cell culture tube.

[0018] 3. The present invention rotates the rotating disk, which drives the arc-shaped slide to rotate, thereby driving the protruding block to rotate along the arc-shaped slide, and then driving a plurality of clamping plates along the circular disk to move closer to the center position of the test tube placement box, so that the plurality of clamping plates clamp and fix the cell culture test tube. At the same time, the clamping force of the plurality of clamping plates can be controlled according to the angle of the rotating disk, thereby clamping cell culture test tubes of different sizes.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 The three-dimensional structure of the present invention Figure 1 ;

[0022] Figure 2 The three-dimensional structure of the present invention Figure 2 ;

[0023] Figure 3 It is an enlarged view of point A of the present invention;

[0024] Figure 4 It is an enlarged view of point B of the present invention;

[0025] Figure 5 is a cross-sectional view of the present invention;

[0026] Figure 6 It is a partial structural diagram of the present invention;

[0027] Figure 7 This is an enlarged exploded view of point C of the present invention;

[0028] Figure 8 It is a top view of the present invention.

[0029] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0030] 1. Incubator; 2. Rotating shaft; 3. Chamber door; 4. Rotating shaft; 5. Rod; 6. Slider; 7. Rack; 8. Connecting rod; 9. Gas rod; 10. Gas cylinder; 11. Air guide tube; 12. One-way control valve; 13. Disinfection water tank; 14. Pressure valve tube; 15. Diverter plate; 16. Fixed plate; 17. Test tube box; 18. Disc; 19. Clamping plate; 20. Protrusion; 21. Arc chute; 22. Rotating disc; 23. First gear; 24. Gear belt; 25. Second gear; 26. Chute; 27. Nozzle; 28. Transparent observation window. DETAILED DESCRIPTION

[0031] The following will clearly and completely describe the technical solutions in the embodiments of the invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of the invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0032] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "top", "middle", "inside" and the like indicating orientation or positional relationship are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the invention.

[0033] See also Figure 1-8 As shown, the present invention is a culture device for biological cell tissue, including an incubator 1 and a rotating shaft 2, the rotating shaft 2 is rotatably installed inside the incubator 1, the rotating shaft 2 is fixedly installed with a box door 3, the rotating shaft 2 is provided with a transmission mechanism, the transmission mechanism is engaged with a rack 7, the rack 7 is fixedly installed with a connecting rod 8, the connecting rod 8 is close to the gas rod 9, the gas rod 9 is slidably connected to the inside of the gas cylinder 10, the front end of the gas cylinder 10 is provided with an air guide tube 11, the front end of the air guide tube 11 is installed with a one-way control valve 12, the front end of the air guide tube 11 is connected to a disinfection water tank 13, the front side of the disinfection water tank 13 is connected to a pressure valve pipe 14, the lower end of the pressure valve pipe 14 is connected to a diverter plate 15, a rotating shaft 4 is provided inside the incubator 1, one end of a rod 5 is rotatably installed on the rotating shaft 4, and a slider 6 is rotatably installed on the other end of the rod 5, the slider 6 is slidably connected to the box door 3, the rotating shaft 4 is provided with a fixed plate 16, and a test tube placement mechanism is provided inside the fixed plate 16.

[0034] The working principle of the biological cell tissue culture device proposed by the present invention is as follows: when using the device, first, the box door 3 is opened, thereby driving the rotating shaft 2 to rotate, and then the rack 7 is moved toward the box door 3 through the transmission mechanism, thereby driving the connecting rod 8 to move. Since the connecting rod 8 is in close contact with one end of the gas rod 9, the gas rod 9 is displaced forward horizontally, and the external air is injected into the interior of the disinfection water tank 13 through the air guide tube 11 through the cooperation between the gas rod 9 and the gas cylinder 10;

[0035] After air is injected into the disinfection water tank 13, the pressure inside it will rise. At this time, since the disinfection water tank 13 and the diverter plate 15 are connected through the pressure valve pipe 14, when the pressure inside the disinfection water tank 13 reaches the preset pressure value of the pressure valve pipe 14, the pressure valve pipe 14 will automatically open, and the pressure valve pipe 14 will cause the disinfection water tank 13 to be connected with the diverter plate 15, that is, the disinfection water inside the disinfection water tank 13 is quickly injected into the diverter plate 15 through the pressure valve pipe 14. Since the diverter plate 15 is fixedly installed in an embedded manner above the incubator 1, and the diverter plate 15 and the door 3 are on the same horizontal line, as the door 3 is rotated to open, the diverter plate 15 will automatically spray the disinfection water in the form of mist downwards. At this time, the disinfection water in the form of mist will form a layer of atomized protective layer of disinfection water, thereby reducing the bacteria or impurities in the external environment from entering the interior of the incubator 1 after the door 3 is opened.

[0036] During the above operation, when the door 3 is opened, the slider 6 is synchronously driven to slide along the inner side of the door 3, thereby driving the rod 5 to rotate inside the incubator 1. Since the rod 5 is fixedly mounted with the fixing plate 16, the fixing plate 16 can be driven to rotate, so that the fixing plate 16 is rotated to the outside of the incubator 1. At this time, the test tube placement mechanism can be disinfected by the disinfectant mist, avoiding the operator having to reach into the incubator 1 to take the cell culture tubes.

[0037] Similarly, after the cell culture tubes are placed, the door 3 is closed to drive the above-mentioned device to reset.

[0038] In one embodiment, for the above-mentioned test tube placement mechanism, the test tube placement mechanism includes a test tube placement box 17 arranged on a fixed plate 16, the test tube placement box 17 is fixedly installed on the fixed plate 16, a disc 18 is fixedly installed inside the test tube placement box 17, a clamping plate 19 is slidably connected above the disc 18, a protrusion block 20 is fixedly installed above the clamping plate 19, the protrusion block 20 is slidably connected to the inside of the arc-shaped slide groove 21, the arc-shaped slide groove 21 is opened on the rotating disk 22, and the rotating disk 22 is rotatably installed inside the test tube placement box 17.

[0039] The working principle of the biological cell tissue culture device proposed in the present invention is to first place the cell culture test tube into the test tube placement box 17, and then rotate the rotating disk 22 to drive the arc-shaped slide 21 to rotate, thereby driving the protrusion 20 to rotate along the arc-shaped slide 21, and then drive several clamping plates 19 along the circular disk 18 to move closer to the center position of the test tube placement box 17, so that the several clamping plates 19 clamp and fix the cell culture test tube. At the same time, the clamping force of the several clamping plates 19 can be controlled according to the angle of the rotating disk 22, so that cell culture test tubes of different sizes can be clamped.

[0040] In one embodiment, for the above-mentioned clamping plate 19, a plurality of clamping plates 19, protruding blocks 20 and arc-shaped sliding grooves 21 are provided, and their parts and installation methods are the same.

[0041] In one embodiment, the transmission mechanism includes a first gear 23 provided on the rotating shaft 2, the first gear 23 is fixedly mounted on the first gear 23, the first gear 23 is connected to the second gear 25 via a gear belt 24, and the second gear 25 is meshed with the rack 7.

[0042] The working principle of the biological cell tissue culture device proposed in the present invention is to open the box door 3, thereby driving the first gear 23 to rotate, and then driving the second gear 25 to rotate through the gear belt 24 transmission connection, thereby realizing the above working principle.

[0043] In one embodiment, for the above-mentioned rotating shaft 4, the rotating shaft 4 is rotatably installed inside the incubator 1, the slider 6 is slidably connected inside the slide groove 26, the slide groove 26 is opened on the inner side of the box door 3, and the fixed plate 16 is fixedly installed on the rotating shaft 4.

[0044] In one embodiment, for the above-mentioned air duct 11, the air duct 11 is connected to the front end of the gas cylinder 10, the disinfection water tank 13 is fixedly installed on the incubator 1, the diverter plate 15 is fixedly installed on the incubator 1, and a nozzle 27 is provided at the bottom of the diverter plate 15, and there are several nozzles 27.

[0045] In one embodiment, the door 3 is provided with a transparent observation window 28 , and the door 3 and the incubator 1 can be made of stainless steel.

[0046] In one embodiment, for the above-mentioned door 3 , a sealing strip is provided between the door 3 and the incubator 1 .

[0047] The working principle of the biological cell tissue culture device proposed by the present invention is that the disinfectant water is sprayed downward in a mist form through the nozzle 27 at the diverter plate 15, and the sealing strip can increase the sealing between the box door 3 and the incubator 1.

[0048] Throughout this specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0049] The preferred embodiments of the invention disclosed above are intended only to help illustrate the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A culture device for biological cell tissue, comprising an incubator (1) and a rotating shaft (2), characterized in that: The rotating shaft (2) is rotatably mounted inside the incubator (1), the rotating shaft (2) is fixedly mounted with a box door (3), the rotating shaft (2) is provided with a transmission mechanism, the transmission mechanism is engaged with a rack (7), the rack (7) is fixedly mounted with a connecting rod (8), the connecting rod (8) is tightly attached to the gas rod (9), the gas rod (9) is slidably connected to the inside of the gas cylinder (10), the front end of the gas cylinder (10) is provided with an air guide tube (11), the front end of the air guide tube (11) is installed with a one-way control valve (12), the front end of the air guide tube (11) is provided with a one-way control valve (12), and the front end of the air guide tube (11) is provided with a one-way control valve (12). The end is connected to a disinfection water tank (13), the front side of the disinfection water tank (13) is connected to a pressure valve pipe (14), the lower end of the pressure valve pipe (14) is connected to a diverter plate (15), a rotating shaft (4) is provided inside the incubator (1), one end of a rod (5) is rotatably mounted on the rotating shaft (4), the other end of the rod (5) is rotatably mounted on a slider (6), the slider (6) is slidably connected to the box door (3), the rotating shaft (4) is provided with a fixed plate (16), and a test tube placement mechanism is provided inside the fixed plate (16).

2. A biological cell tissue culture device according to claim 1, characterized in that: The test tube placement mechanism comprises a test tube placement box (17) arranged on a fixed plate (16), the test tube placement box (17) is fixedly mounted on the fixed plate (16), a disc (18) is fixedly mounted inside the test tube placement box (17), a clamping plate (19) is slidably connected above the disc (18), a protruding block (20) is fixedly mounted above the clamping plate (19), the protruding block (20) is slidably connected inside an arc-shaped chute (21), the arc-shaped chute (21) is opened on a rotating disk (22), and the rotating disk (22) is rotatably mounted inside the test tube placement box (17).

3. A biological cell tissue culture device according to claim 2, characterized in that: The clamping plates (19), protruding blocks (20) and arc-shaped sliding grooves (21) are all provided in plurality, and their parts and installation methods are the same.

4. The biological cell tissue culture device according to claim 1, characterized in that: The transmission mechanism comprises a first gear (23) arranged on a rotating shaft (2), the first gear (23) being fixedly mounted on the first gear (23), the first gear (23) being connected to a second gear (25) via a gear belt (24), and the second gear (25) being meshed with a rack (7).

5. The biological cell tissue culture device according to claim 4, characterized in that: The rotating shaft (4) is rotatably mounted inside the incubator (1), the slider (6) is slidably connected inside a chute (26), the chute (26) is opened on the inner side of the box door (3), and the fixed plate (16) is fixedly mounted on the rotating shaft (4).

6. The biological cell tissue culture device according to claim 1, characterized in that: The air guide tube (11) is connected to the front end of the air cylinder (10), the disinfection water tank (13) is fixedly mounted on the incubator (1), the diverter plate (15) is fixedly mounted on the incubator (1), and a nozzle (27) is provided at the bottom of the diverter plate (15), and a plurality of nozzles (27) are provided.

7. The biological cell tissue culture device according to claim 6, characterized in that: The box door (3) is provided with a transparent observation window (28), and the box door (3) and the incubator (1) can be made of stainless steel.

8. The biological cell tissue culture device according to claim 6, characterized in that: A sealing strip is provided between the box door (3) and the incubator (1).

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