Lung cancer tumor sampling device and method

Abstract

The invention relates to the technical field of medical equipment, in particular to a lung cancer tumor sampling device and method.The lung cancer tumor sampling device comprises multiple needle cores and multiple sleeves in coaxial sliding fit with the needle cores, the multiple needle cores and the multiple sleeves are in one-to-one correspondence, and the multiple needle cores are parallel; the ends, back to the sampling groove, of the sleeves are jointly connected with a shell of a hollow structure, a push plate is slidably connected into the shell in the axial direction of the needle cores, and the ends, back to the sampling groove, of the needle cores penetrate through the shell and are inserted into the shell to be connected with the push plate; and an adjusting part for driving the push plate to slide in the shell is arranged between the shell and the push plate. The problem that in the prior art, single-point sampling may miss a key mutation area, and false negative is caused is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a lung cancer tumor sampling device and method. Background Art

[0002] Lung cancer, the malignant tumor with the highest morbidity and mortality worldwide, is primarily classified by pathological type into non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Due to the significant spatial heterogeneity of lung cancer, different regions of the same tumor lesion may exhibit differential expression of driver genes such as EGFR, ALK, and KRAS. This molecular heterogeneity directly impacts the selection of clinical treatment options and the evaluation of treatment efficacy. Therefore, obtaining representative tumor tissue samples is crucial for achieving accurate pathological diagnosis and personalized treatment.

[0003] Currently, commonly used lung cancer sampling techniques in clinical practice include percutaneous lung puncture biopsy, bronchoscopic biopsy, and ex vivo sampling after surgical resection. Among them, percutaneous lung puncture biopsy is an important diagnostic method, and its operation process is as follows:

[0004] First, the tumor location is determined and the puncture path is planned using CT or ultrasound imaging guidance;

[0005] Subsequently, a single-core biopsy needle (such as a Tru-Cut needle) is inserted into the target area along a predetermined path;

[0006] During the tissue capture phase, the operator first pushes the needle core out of the cannula to expose the sample well, then pushes the cannula forward while retracting the needle core. The tissue strip is cut and captured through mechanical squeezing (the relative movement of the cannula and needle core creates a scissor-like closing effect).

[0007] After the sampling is completed, the puncture needle is removed and the obtained tissue strip is subjected to pathological analysis;

[0008] When the sample volume is insufficient, the puncture operation usually needs to be repeated 3-5 times.

[0009] However, a single puncture can only obtain a single-point sample, and multiple punctures are required to cover tumor heterogeneity, which prolongs the operation time. Moreover, single-point sampling may miss key mutation areas (such as only obtaining necrotic tissue), resulting in false negatives. Summary of the Invention

[0010] In view of this, the purpose of the present invention is to provide a lung cancer tumor sampling device and method to solve the problem in the prior art that single-point sampling may miss key mutation areas and cause false negatives.

[0011] The present invention is achieved through the following technical solutions:

[0012] A lung cancer tumor sampling device comprises a needle core and a cannula coaxially slidingly engaged with the needle core, wherein a plurality of needle cores and a plurality of cannulae are provided, the plurality of needle cores correspond to the plurality of cannulae one by one, and the plurality of needle cores are parallel;

[0013] The ends of the multiple sleeves facing away from the sampling slot are commonly connected to a hollow housing, a push plate is slidably connected to the housing along the axial direction of the needle core, and the ends of the multiple needle cores facing away from the sampling slot pass through the housing, are inserted into the housing, and are connected to the push plate;

[0014] An adjusting portion for driving the push plate to slide in the shell is provided between the shell and the push plate.

[0015] Furthermore, the plurality of sleeves are arranged in a linear array, and the arrangement direction is perpendicular to the sleeve axis, and the plurality of sleeves are divided into two symmetrical collection parts;

[0016] Two connecting bars corresponding to the two collecting parts are provided in the shell, and the multiple sleeves in the collecting parts are detachably fixedly connected to the corresponding connecting bars;

[0017] The two connecting strips are both rotatably connected to the inner wall of one end of the housing facing the sampling slot, and the rotation center line is parallel to the needle core;

[0018] The needle core is slidably connected to the baffle, and the sliding track coincides with the corresponding sleeve rotation track.

[0019] Furthermore, the connecting strip is provided with a plurality of insertion holes corresponding to the plurality of sleeves one by one, and the sleeves are inserted into the insertion holes at one end facing away from the sampling slot.

[0020] Furthermore, one end of the connecting strip is provided with two coaxial arc-shaped sliding holes, and the axes of the arc-shaped sliding holes are coplanar with the axes of the multiple needle cores in the corresponding collection part;

[0021] A sliding rod is provided in each of the two arc-shaped sliding holes. One end of the sliding rod extends axially along the needle core and is fixedly connected to the end wall of the shell. The outer circular surface of the sliding rod is in contact with the two radial side walls of the arc-shaped sliding hole.

[0022] Furthermore, the two connecting bars are fixedly connected to outer gear rings at opposite ends, the axes of the outer gear rings overlap with the rotation center lines of the corresponding connecting bars, and the two outer gear rings are meshed;

[0023] A limiting portion for limiting the rotation of the outer gear ring is provided between the outer gear ring and the housing.

[0024] Furthermore, the limiting portion includes a limiting block embedded in the top wall of the housing, and a first protrusion adapted to the tooth groove of the outer gear ring is provided on a side of the limiting block facing the outer gear ring, and the first protrusion is inserted into the tooth groove of the outer gear ring;

[0025] The limit block is radially slidably engaged with the top wall of the shell along the outer gear ring. The limit block is fixedly connected to a side facing away from the outer gear ring with a second protrusion, and when the first protrusion is embedded in the tooth groove of the outer gear ring, the second protrusion protrudes from the outer top surface of the shell.

[0026] Furthermore, a handle with an open end and a hollow structure is provided at one end of the housing facing away from the sampling slot, the open end of the handle is detachably fixedly connected to the housing, and the interior of the handle is in communication with the interior of the housing;

[0027] The adjusting portion includes a compression spring disposed in the housing, a rack parallel to the needle core, and a gear meshing with the rack. One end of the compression spring abuts against the end wall of the housing facing the sampling slot, and the other end abuts against the push plate. When the compression spring is in a naturally extended state, the sampling slot on the needle core is retracted into the sleeve and covered and sealed.

[0028] One end of the rack is fixedly connected to the push plate, and the other end is inserted into the handle and slidably engaged with the inner wall of the handle;

[0029] The two ends of the gear are respectively inserted into the two side walls of the handle and rotated together. A shift rod is provided on the side of the gear facing away from the rack. One end of the shift rod is fixedly connected to the outer circular surface of the gear, and the other end passes through the side wall of the handle along the radial direction of the gear and extends out of the handle.

[0030] Furthermore, the gear is provided with a rack below, a limit groove is provided on the top surface of the rack, a rotation bar is provided above the rack, the middle portion of the rotation bar is rotatably connected to the inner wall of the handle, and the rotation center line is parallel to the rotation center line of the gear;

[0031] The bottom end of the rotating bar extends downwardly in the direction of the sampling slot and abuts against the top surface of the rack, and the top end passes through the top wall of the handle and extends out of the handle. When the bottom end of the rotating bar is inserted into the limiting groove, the angle between the rotating bar and the rack is acute.

[0032] A method for sampling lung cancer tumors, comprising using the above-mentioned device, and the following steps:

[0033] S1. Preoperative localization: Determine the tumor location and puncture path through imaging examination;

[0034] S2, array puncture: multiple needle cores are inserted into the tumor tissue simultaneously, with the sampling grooves of each needle core located in different areas of the tumor;

[0035] S3, step-by-step sampling:

[0036] S31, pushing the push plate through the adjustment part to move the needle core forward, and each needle core sampling groove is moved out of the corresponding sleeve and exposed;

[0037] S32: Keeping the cannula in a fixed position, the needle core is pulled back by the push plate, and the barbs on the front edge of the sampling groove are used to anchor the tumor tissue, and the cannula front end is used to shear the tumor tissue, and the sheared tumor tissue is stored in the sampling groove;

[0038] S4. Sample recovery: Pull out the needle core and cannula as a whole, push the push plate again through the adjustment part to move the needle core forward, expose the sampling grooves on each needle core, remove the samples in multiple sampling grooves, and record them by number;

[0039] S5. Heterogeneity analysis: Molecular testing is performed on samples obtained from each needle core.

[0040] Furthermore, the step S2 specifically includes:

[0041] S21. Rotate the two connecting bars to adjust their relative positions and fix them, for example, the two connecting bars are collinear / parallel / intersecting in their extended lines, and the corresponding plurality of needle cores are arranged in a straight line / matrix / radially.

[0042] S22. Synchronously inserting multiple needle cores into the tumor tissue, with the sampling grooves of the needle cores located in different areas of the tumor.

[0043] The beneficial effects of the present invention are:

[0044] The present invention relates to a lung cancer tumor sampling device and method. A plurality of cannulas are provided on a housing, and a plurality of needle cores corresponding to the plurality of cannulas are provided on a push plate. An adjustment portion is used to drive the push plate to slide on the housing, thereby achieving synchronous movement of the plurality of needle cores on the corresponding cannulas. Multiple locations of tumor tissue are sampled simultaneously, reducing the risk of false negatives caused by the sampling point deviating from the key mutation area.

[0045] Other advantages, objects, and features of the present invention will be described in part in the following description and, in part, will be apparent to those skilled in the art upon examination of the following description or may be learned from practice of the present invention. The objects and other advantages of the present invention may be realized and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 Schematic diagram of the three-dimensional structure of an embodiment of the present invention (state 1);

[0047] Figure 2 Schematic diagram of the three-dimensional structure of an embodiment of the present invention (state 2);

[0048] Figure 3 Schematic diagram of the three-dimensional structure of an embodiment of the present invention (state three);

[0049] Figure 4 An exploded view of an embodiment of the present invention;

[0050] Figure 5 A top view of an embodiment of the present invention;

[0051] Figure 6 for Figure 5 Cross-sectional view of AA;

[0052] Figure 7 for Figure 6 Enlarged view of point C in the middle;

[0053] Figure 8 for Figure 5 Cross-sectional view of the middle BB;

[0054] Figure 9 Schematic diagram of the three-dimensional structure of the housing in an embodiment of the present invention;

[0055] Figure 10 Schematic diagram of the three-dimensional structure of the rotating bar in an embodiment of the present invention;

[0056] Figure 11 Schematic diagram of the three-dimensional structure of the limiting block in an embodiment of the present invention;

[0057] Figure 12 Schematic diagram of the three-dimensional structure of the sleeve in an embodiment of the present invention;

[0058] Figure 13 Schematic diagram of the three-dimensional structure of the push plate and the needle core in an embodiment of the present invention;

[0059] Figure 14 Schematic diagram of the three-dimensional structure of the needle core in an embodiment of the present invention;

[0060] Figure 15 Schematic diagram of the three-dimensional structure of the handle in an embodiment of the present invention.

[0061] In the figure: 1. needle core; 11. sampling slot; 2. sleeve; 3. shell; 31. connecting strip; 311. jack; 312. arc-shaped sliding hole; 313. slide rod; 32. outer gear ring; 33. limit block; 331. first protrusion; 332. second protrusion; 4. push plate; 41. guide hole; 42. slider; 421. threaded hole; 5. grip; 51. cover plate; 61. compression spring; 62. rack; 621. limit slot; 63. gear; 64. lever; 65. rotating bar. DETAILED DESCRIPTION

[0062] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0063] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0064] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not require further definition or explanation in subsequent drawings.

[0065] In the above description of the present invention, it should be noted that the terms "one side," "the other side," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, or the orientations or positional relationships in which the inventive product is typically placed when in use. These terms are intended solely to facilitate the description of the present invention and simplify the description, and are not intended to indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first," "second," etc., etc., are used solely for distinction and should not be construed as indicating or implying relative importance.

[0066] Furthermore, the term "identical" and similar terms do not necessarily require that the components be absolutely identical; slight variations are permitted. The term "perpendicular" simply refers to the positional relationship between components being more perpendicular than "parallel," not that the structure must be perfectly vertical; rather, it can be slightly tilted.

[0067] See also Figure 1-15 The present invention provides a technical solution: a lung cancer tumor sampling device, comprising a needle core 1 and a cannula 2 coaxially slidingly matched with the needle core 1, wherein a plurality of the needle cores 1 and the cannula 2 are provided, the plurality of the needle cores 1 corresponds to the plurality of cannula 2 one by one, and the plurality of the needle cores 1 are parallel;

[0068] The ends of the multiple cannulas 2 facing away from the sampling slot 11 are commonly connected to a hollow housing 3, and a push plate 4 is connected to the housing 3 along the axial direction of the needle core 1. The ends of the multiple needle cores 1 facing away from the sampling slot 11 pass through the housing 3, are inserted into the housing 3, and are connected to the push plate 4.

[0069] An adjusting portion is provided between the housing 3 and the push plate 4 for driving the push plate 4 to slide in the housing 3 .

[0070] In this solution, multiple cannulas 2 are positioned on a housing 3, and multiple needle cores 1 corresponding to these cannulas 2 are positioned on a push plate 4. An adjustment mechanism drives the push plate 4 to slide on the housing 3, enabling the simultaneous movement of multiple needle cores 1 along their corresponding cannulas 2, allowing for simultaneous sampling of multiple locations within tumor tissue. Compared to traditional single-needle sampling devices, this device uses the push plate 4 to propel multiple needle cores 1 in synchronous motion for sampling, simultaneously sampling multiple locations. This expands the sampling coverage area and significantly reduces the risk of false negatives due to sampling points straying from critical mutation regions.

[0071] Among them, the needle core 1 and the push plate 4 can be fixedly connected together by bonding, threading, etc.; the sleeve 2 and the shell 3 can be fixedly connected together by bonding, threading, etc.; the shell 3 has a semi-elliptical cylindrical shape, and the push plate 4 has a semi-elliptical plate shape and is adapted to the interior of the shell 3. After the push plate 4 is embedded in the shell 3, the outer contour of the push plate 4 fits the inner wall of the shell 3, and the push plate 4 has a structural thickness that can limit the push plate 4 from tipping over. The push plate 4 can only slide in a single degree of freedom along the axial direction of the needle core 1 in the shell 3, so that the overall structure of the device is compact and the structural stability is high. Figure 9 、 13 shown.

[0072] The front end of the needle core 1 (the end that is inserted into the tumor tissue) is sharp, which plays the role of guiding and assisting the needle core 1 to be inserted into the tumor tissue. The outer surface of the front end of the needle core 1 is provided with a sampling groove 11 for storing the sheared and separated tumor tissue; the edge of the front end of the sampling groove 11 is provided with a relatively sharp conical barb, such as Figure 14 As shown, the needle core 1 is used to anchor the tumor tissue during the process of retraction into the cannula 2, and the front edge of the cannula 2 is used to shear the tumor tissue. The principle of the relative movement of the needle core 1 and the cannula 2 to shear the tumor tissue is a mature technology and will not be elaborated here.

[0073] Alternatively, the adjustment portion may be configured as a motor and a screw. The motor is fixedly mounted on the end of the housing 3 facing away from the sampling slot 11. One end of the screw is connected to the motor output end via a coupling or the like. The other end of the screw extends axially along the needle core 1, sequentially penetrating the end wall of the housing 3 and the push plate 4. The screw and the housing 3 are rotationally coupled, and the screw and the push plate 4 are threadedly coupled. While the screw is restricted from axial movement, the motor is simply started to drive the needle core 1 and the corresponding cannula 2 to relative motion, thereby performing sampling.

[0074] When using, the specific steps are as follows:

[0075] Preoperative localization (imaging guidance): Using imaging methods such as CT, ultrasound, or MRI, the tumor's location, size, and optimal puncture path are determined to avoid important blood vessels and organs;

[0076] Device inspection and disinfection: Check that the internal structure of the device is stable and operates smoothly (such as the motor screw mechanism), and sterilize the device to avoid infection risks;

[0077] Puncture (array puncture): multiple cannulas 2 are synchronously inserted into the tumor tissue along a predetermined puncture path, so that the sampling grooves 11 of each needle core 1 are distributed in different areas of the tumor (such as the edge, center, and junction);

[0078] Stepwise sampling (tissue shearing): The adjustment unit is activated (e.g., a motor drives the screw in forward rotation), pushing the push plate 4 to move the needle cores 1 forward. The sampling slots 11 of each needle core 1 extend from the cannula 2 and are exposed to the tumor tissue. Then, while the cannula 2 is fixed, the push plate 4 is pulled back (e.g., a motor drives the screw in reverse rotation). The barbs at the front end of the sampling slots 11 hook the tissue, and the tumor sample is sheared under the squeezing action of the front end of the cannula 2. The sample remains in the sampling slots 11 until the sampling slots 11 are completely covered and sealed by the cannula 2.

[0079] Sample recovery and labeling: Pull the needle core 1 and cannula 2 out of the body as a whole. Push the push plate 4 again to move the needle core 1 forward to expose the sampling slots 11. Use tweezers or special tools to remove tissue samples from each slot. Label the samples according to the spatial arrangement of the needle core 1 (e.g., numbered 1-N corresponds to from left to right, from outside to inside, etc.), and record their anatomical positions.

[0080] Heterogeneity analysis: Pathological examination or molecular testing (such as gene mutation and PD-L1 expression) is performed on tissues at each sampling point to assess the heterogeneity within the tumor.

[0081] In this embodiment: the plurality of sleeves 2 are arranged in a linear array, and the arrangement direction is perpendicular to the axis of the sleeve 2, and the plurality of sleeves 2 are divided into two symmetrical collection parts;

[0082] Two connecting strips 31 corresponding to the two collecting parts are provided in the housing 3, and the multiple sleeves 2 in the collecting part are detachably fixedly connected to the corresponding connecting strips 31;

[0083] The two connecting strips 31 are both rotatably connected to the inner wall of one end of the housing 3 facing the sampling slot 11, and the rotation center line is parallel to the needle core 1;

[0084] The needle core 1 is slidably connected to the baffle, and the sliding trajectory coincides with the rotation trajectory of the corresponding sleeve 2.

[0085] In this solution, the multiple cannulas 2 are designed as two symmetrical collection sections, and a rotatable connecting strip 31 is used for flexible adjustment, so that the arrangement of the multiple cannulas 2 can be adjusted and the sampling layout can be adjusted. The two collection sections can be independently adjusted in angle (e.g., 0° to 90°), making the arrangement of the cannulas 2 diverse and capable of presenting the following three usage states:

[0086] State 1 (such as Figure 1 As shown): multiple sleeves 2 are arranged in a straight line (two connecting strips 31 are collinear), which is suitable for linear sampling of long tumors;

[0087] State 2 (such as Figure 2 As shown): multiple sleeves 2 are arranged in a V-shape or radial shape (the extension lines of the two connecting strips 31 intersect), which is suitable for multi-region coverage of dispersed tumors;

[0088] State 3 (such as Figure 3 As shown): Multiple sleeves 2 are arranged in a matrix (two connecting strips 31 are parallel), which can perform grid-based zoning sampling on the tumor cross section, which is particularly suitable for tumors with large volume (>3cm) or significant heterogeneity, significantly reducing the risk of missed detection.

[0089] In addition, the rear end of the sleeve 2 (the end facing away from the sampling slot 11) can be inserted into the connecting strip 31 and connected through threaded fit to achieve the purpose of detachability. The sleeve 2 can be disassembled and installed according to actual use needs to facilitate sterilization of the sleeve 2. In addition, a plurality of avoidance holes corresponding to the plurality of sleeves 2 are opened on the end wall of the housing 3 facing the sampling slot 11. The sleeve 2 is inserted into the corresponding avoidance hole and can rotate normally. Figure 2 shown.

[0090] In this embodiment, a plurality of insertion holes 311 corresponding to the plurality of sleeves 2 are formed on the connecting bar 31 , and one end of the sleeve 2 facing away from the sampling slot 11 is inserted into the insertion hole 311 .

[0091] In this solution, the insertion hole 311 can be designed as a relatively flat cylindrical hole, such as Figure 9 As shown, the sleeve 2 is tightly inserted into the socket 311 to achieve the purpose of fixing the sleeve 2, which is simple and convenient to operate. Alternatively, the sleeve 2 can be designed as a circular hole with internal threads on the inner surface. The sleeve 2 can be fixed by inserting the rear end of the sleeve into the socket 311 and connecting through the threads. In this case, the tight-fitting method is preferred.

[0092] The baffle is provided with guide holes 41 corresponding to the multiple needle cores 1 in the collection part. A slider 42 is slidably fitted in the guide hole 41, and the sliding track of the slider 42 coincides with the rotation track of the corresponding sleeve 2 (needle core 1). When the needle core 1 is connected to the corresponding slider 42, the connecting strip 31 can normally drive the needle core 1 to move, changing the layout of the needle core 1, such as Figure 13 shown.

[0093] In addition, a threaded hole 421 can be provided on the slider 42, and an external thread can be provided on the outer circumferential surface of the rear end of the needle core 1 (the end facing away from the sampling groove 11). By inserting the rear end of the needle core 1 into the threaded hole 421 and connecting them with the threaded connection, the purpose of detachment is achieved, so that the cannula 2 and the needle core 1 can be removed from the device for easy maintenance and replacement, or the number of cannula 2 and needle core 1 can be increased or decreased according to actual usage, thereby changing the number and spacing of sampling points. At the same time, the cannula 2 and the needle core 1 can be used as disposable products, reducing the risk of cross infection caused by incomplete sterilization and disinfection. The housing 3 and other components do not come into contact with the patient's skin, so the risk of cross infection is low. Therefore, they can be recycled after sterilization and disinfection, reducing the cost of use.

[0094] In this embodiment: one end of the connecting strip 31 is provided with two coaxial arc-shaped sliding holes 312, and the axis of the arc-shaped sliding hole 312 is coplanar with the axis of the multiple needle cores 1 in the corresponding collection part;

[0095] A sliding rod 313 is provided in each of the two sections of the arc-shaped sliding hole 312. One end of the sliding rod 313 extends axially along the needle core 1 and is fixedly connected to the end wall of the housing 3. The outer circular surface of the sliding rod 313 is in contact with the two radial side walls of the arc-shaped sliding hole 312.

[0096] In this solution, the outer surface of the slide rod 313 is in contact with the radial side walls of the arc-shaped slide hole 312, and two slide rods 313 are provided, so that the slide rod 313 can only slide along the length direction of the arc-shaped slide hole 312, and the connecting bar 31 can only rotate around the axial direction of the arc-shaped slide hole 312. Figure 9 、 10 The free end of the slide bar 313 extends through the arc-shaped slide hole 312 and out of the arc-shaped slide hole 312. The end of the slide bar 313 can limit the axial movement of the connecting bar 31 along the slide bar 313 by inserting a pin, threading and matching a connecting nut, etc., so as to achieve the purpose of rotationally connecting the connecting bar 31 and the housing 3.

[0097] In this embodiment, the two connecting bars 31 are fixedly connected to the outer gear rings 32 at the opposite ends. The axis of the outer gear rings 32 overlaps with the rotation center line of the corresponding connecting bar 31, and the two outer gear rings 32 are meshed.

[0098] A limiting portion for limiting the rotation of the outer gear ring 32 is provided between the outer gear ring 32 and the housing 3 .

[0099] In this scheme, if Figure 4As shown, two connecting bars 31 are connected and driven by two outer gear rings 32. When one connecting bar 31 rotates, the other connecting bar 31 rotates in the opposite direction. When the sampling layout needs to be adjusted, the restraints on the connecting bars are released, and the outer gear rings mesh to achieve symmetrical angle adjustment, improving adjustment efficiency. The two outer gear rings 32 also support and limit each other, further improving the structural stability of the device.

[0100] In this embodiment, the limiting portion includes a limiting block 33 embedded in the top wall of the housing 3. The limiting block 33 is provided with a first protrusion 331 adapted to the tooth groove of the outer gear ring 32 on a side facing the outer gear ring 32. The first protrusion 331 is inserted into the tooth groove of the outer gear ring 32.

[0101] The limit block 33 is radially slidably engaged with the top wall of the shell 3 along the outer ring gear 32. The limit block 33 is fixedly connected to a side facing away from the outer ring gear 32 with a second protrusion 332, and when the first protrusion 331 is embedded in the tooth groove of the outer ring gear 32, the second protrusion 332 protrudes from the outer top surface of the shell 3.

[0102] In this solution, a concave cavity is provided on the inner top wall of the shell 3 for accommodating the slider 42, and the limit block 33 is embedded in the concave cavity and tightly connected (interference fit). In the absence of external force, friction can be used to limit the limit block 33 from sliding in the concave cavity (radially sliding along the outer gear ring 32).

[0103] When in use, when the positions of the two connecting strips 31 need to be fixed, it is only necessary to press the second protrusion 332 outside the shell 3 to make the limit block 33 slide close to the outer ring gear 32, so that the first protrusion 331 is inserted into the tooth groove of the outer ring gear 32 to form an engaged state. Due to the interference of the side wall of the concave cavity, the limit block 33 is limited to move along the tangential direction of the circumference of the outer ring gear 32, so that the limit block 33 and the outer ring gear 32 form a clamping state, thereby limiting the rotation of the connecting strip 31.

[0104] When the relative positions of the two connecting bars 31 need to be adjusted, just hold the second protrusion 332 and pull it out of the housing 3 to slide the limit block 33 away from the outer gear ring 32. After the first protrusion 331 is removed from the tooth groove, the locking state is eliminated and the connecting bar 31 can rotate normally. Figure 8 、 11 shown.

[0105] In this embodiment, a handle 5 having a hollow structure with an open end is provided at one end of the housing 3 facing away from the sampling slot 11. The open end of the handle 5 is detachably fixed to the housing 3, and the interior of the handle 5 is in communication with the interior of the housing 3.

[0106] The adjusting portion includes a compression spring 61 disposed within the housing 3, a rack 62 parallel to the needle core 1, and a gear 63 meshing with the rack 62. One end of the compression spring 61 abuts against the end wall of the housing 3 facing the sampling slot 11, and the other end abuts against the push plate 4. When the compression spring 61 is in a naturally extended state, the sampling slot 11 on the needle core 1 is retracted into the cannula 2 and covered and blocked.

[0107] One end of the rack 62 is fixedly connected to the push plate 4, and the other end is inserted into the handle 5 and slidably engaged with the inner wall of the handle 5;

[0108] The two ends of the gear 63 are respectively inserted into the two side walls of the handle 5 and rotate together. A shift rod 64 is provided on the side of the gear 63 facing away from the rack 62. One end of the shift rod 64 is fixedly connected to the outer circular surface of the gear 63, and the other end penetrates the side wall of the handle 5 along the radial direction of the gear 63 and extends out of the handle 5.

[0109] In this scheme, if Figure 6 、 15 As shown, the housing 3 is open at one end facing away from the sampling slot 11. A cover plate 51 is provided at the open end of the handle 5 to cover and seal the opening of the housing 3. The cover plate 51 is bolted to the housing 3 to facilitate radial disinfection and maintenance of the components within the housing 3. The handle 5 is provided at the end of the housing 3 facing away from the sampling slot 11. The stopper 33 is located on the sidewall of the housing 3, keeping the handle 5 and the lever 64 away from the stopper 33 to prevent accidental contact with the stopper 33 and its position from being altered.

[0110] The compression spring 61 plays an elastic supporting role to the push plate 4. The handle 5 is provided with a clearance notch on the shift rod 64 that connects the inside and outside of the handle 5.

[0111] During use, the relevant technician holds the handle with his palm, hooks the lever 64 at the outer end of the grip 5 with his index finger, and drives the gear 63 by shaking the lever 64, pushing the push plate 4 and the needle core 1 forward through the rack 62 to expose the sampling slot 11, and the compression spring 61 is squeezed and contracted to store energy; when the finger slides off the lever 64, the resistance to the lever 64 disappears, the spring releases energy and rebounds quickly, prompting the push plate 4 to drive the needle core 1 to move backward to shear the tumor tissue.

[0112] In this embodiment, the gear 63 is provided below the rack 62, a limiting groove 621 is provided on the top surface of the rack 62, and a rotating bar 65 is provided above the rack 62. The middle portion of the rotating bar 65 is rotatably connected to the inner wall of the handle 5, and the rotation centerline is parallel to the rotation centerline of the gear 63;

[0113] The bottom end of the rotating bar 65 extends obliquely downward toward the sampling slot 11 and abuts against the top surface of the rack 62. The top end penetrates the top wall of the handle 5 and extends out of the handle 5. When the bottom end of the rotating bar 65 is inserted into the limiting slot 621, the angle between the rotating bar 65 and the rack 62 is acute.

[0114] In this scheme, if Figure 7 As shown, there are multiple limit grooves 621, and the multiple limit grooves 621 are evenly arranged along the length direction of the rack 62. The weight of the section from the top end of the rotating bar 65 to the rotation center line is less than the weight of the section from the bottom end of the rotating bar 65 to the rotation center line, so that the bottom end of the rotating bar 65 naturally droops and is always in conflict with the rack 62.

[0115] When the bottom end of the rotating bar 65 is inserted into the limiting groove 621, the distance from the rotation centerline of the rotating bar 65 to the top surface of the rack 62 is less than the length from the top end of the rotating bar 65 to the rotation centerline. Two sliding grooves are provided at the open end of the handle 5, into which the two side walls of the middle portion of the rack 62 respectively fit, slidingly engaging with each other. Furthermore, one end of the rack 62 is fixedly connected to the push plate 4. Therefore, when the bottom end of the rotating bar 65 is inserted into the limiting groove 621, the rotating bar 65 and the limiting groove 621 are locked, restricting the rack 62 from sliding away from the sleeve 2 (restraining the needle core 1 from moving backward), thereby maintaining the spring in a stored energy state.

[0116] When the relevant technician presses the top of the rotating bar 65 with his thumb, the rotating bar 65 rotates and lifts up the bottom end until it slides out of the limit groove 621, eliminating the stuck state. Under the elastic force of the compression spring 61, the push plate 4 drives the rack 62 and the needle core 1 to move backward together, and the shearing work is automatically performed.

[0117] A method for sampling lung cancer tumors, comprising using the above-mentioned device, and the following steps:

[0118] S1. Preoperative localization: Determine the tumor location and puncture path through imaging examination;

[0119] S2, array puncture: multiple needle cores 1 are synchronously inserted into the tumor tissue, with the sampling grooves 11 of each needle core 1 located in different areas of the tumor;

[0120] S3, step-by-step sampling:

[0121] S31, pushing the push plate 4 by the adjusting portion to move the needle core 1 forward, and the sampling groove 11 of each needle core 1 is moved out of the corresponding sleeve 2 and exposed;

[0122] S32: Keeping the cannula 2 in a fixed position, the needle core 1 is pulled back by the push plate 4, and the barbs on the front edge of the sampling groove 11 are used to anchor the tumor tissue, and the barbs cooperate with the front end of the cannula 2 to shear the tumor tissue, and the sheared tumor tissue is stored in the sampling groove 11;

[0123] S4. Sample recovery: Pull out the needle core 1 and cannula 2 as a whole, push the push plate 4 again through the adjustment part to move the needle core 1 forward, exposing the sampling grooves 11 on each needle core 1, and take out the samples in multiple sampling grooves 11 and record them by number;

[0124] S5. Heterogeneity analysis: molecular testing is performed on the samples obtained from each needle core 1.

[0125] In this embodiment, step S2 specifically includes:

[0126] S21, rotating the two connecting bars 31 to adjust the relative position and fix them, for example, the two connecting bars 31 are collinear / parallel / the extended lines intersect, corresponding to the plurality of needle cores 1 being arranged in a straight line / in a matrix / in a radial arrangement;

[0127] S22 , inserting multiple needle cores 1 into the tumor tissue simultaneously, with the sampling grooves 11 of the needle cores 1 being located in different areas of the tumor.

[0128] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not limiting. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A lung cancer tumor sampling device, comprising a needle core (1) and a sleeve (2) coaxially slidingly engaged with the needle core (1), characterized in that: The needle cores (1) and the sleeves (2) are both provided in plurality, the plurality of needle cores (1) correspond one-to-one to the plurality of sleeves (2), and the plurality of needle cores (1) are parallel; One end of the plurality of sleeves (2) facing away from the sampling slot (11) is commonly connected to a housing (3) having a hollow structure, a push plate (4) is slidably connected to the inside of the housing (3) along the axial direction of the needle core (1), and one end of the plurality of needle cores (1) facing away from the sampling slot (11) passes through the housing (3), is inserted into the housing (3), and is connected to the push plate (4); An adjusting portion for driving the push plate (4) to slide in the shell (3) is provided between the shell (3) and the push plate (4).

2. The lung cancer tumor sampling device according to claim 1, characterized in that: The plurality of sleeves (2) are arranged in a linear array, and the arrangement direction is perpendicular to the axis of the sleeve (2), and the plurality of sleeves (2) are divided into two symmetrical collection parts; Two connecting bars (31) corresponding to the two collecting parts are provided in the housing (3), and the multiple sleeves (2) in the collecting parts are all detachably fixedly connected to the corresponding connecting bars (31); The two connecting strips (31) are both rotatably connected to the inner wall of one end of the housing (3) facing the sampling slot (11), and the rotation center line is parallel to the needle core (1); The needle core (1) is slidably connected to the baffle, and the sliding track coincides with the rotation track of the corresponding sleeve (2).

3. The lung cancer tumor sampling device according to claim 2, characterized in that: The connecting strip (31) is provided with a plurality of insertion holes (311) corresponding one-to-one to the plurality of sleeves (2), and one end of the sleeve (2) facing away from the sampling slot (11) is inserted into the insertion hole (311).

4. The lung cancer tumor sampling device according to claim 2, characterized in that: One end of the connecting strip (31) is provided with two coaxial arc-shaped sliding holes (312), and the axes of the arc-shaped sliding holes (312) are coplanar with the axes of the multiple needle cores (1) in the corresponding collection part; A sliding rod (313) is provided in each of the two arc-shaped sliding holes (312). One end of the sliding rod (313) extends axially along the needle core (1) and is fixedly connected to the end wall of the housing (3). The outer circular surface of the sliding rod (313) is in contact with both radial side walls of the arc-shaped sliding hole (312).

5. The lung cancer tumor sampling device according to claim 2, characterized in that: The two connecting bars (31) are fixedly connected to outer gear rings (32) at opposite ends, the axes of the outer gear rings (32) overlap with the rotation center lines of the corresponding connecting bars (31), and the two outer gear rings (32) are meshed with each other; A limiting portion for limiting the rotation of the outer gear ring (32) is provided between the outer gear ring (32) and the housing (3).

6. The lung cancer tumor sampling device according to claim 5, characterized in that: The limiting portion comprises a limiting block (33) embedded in the top wall of the housing (3); a first protrusion (331) adapted to the tooth groove of the outer gear ring (32) is provided on a side of the limiting block (33) facing the outer gear ring (32); the first protrusion (331) is inserted into the tooth groove of the outer gear ring (32); The limit block (33) is radially slidably engaged with the top wall of the housing (3) along the outer gear ring (32); a second protrusion (332) is fixedly connected to a side of the limit block (33) facing away from the outer gear ring (32); and when the first protrusion (331) is embedded in the tooth groove of the outer gear ring (32), the second protrusion (332) protrudes from the outer top surface of the housing (3).

7. The lung cancer tumor sampling device according to claim 1, characterized in that: The housing (3) is provided with a handle (5) with one end open and a hollow structure at one end, the handle (5) having an open end being detachably fixedly connected to the housing (3), and the interior of the handle (5) is in communication with the interior of the housing (3); The regulating portion comprises a compression spring (61) disposed in the housing (3), a rack (62) parallel to the needle core (1), and a gear (63) meshing with the rack (62); one end of the compression spring (61) abuts against the end wall of the housing (3) facing the sampling slot (11), and the other end abuts against the push plate (4); and when the compression spring (61) is in a naturally extended state, the sampling slot (11) on the needle core (1) is retracted into the sleeve (2) and is covered and sealed; One end of the rack (62) is fixedly connected to the push plate (4), and the other end is inserted into the handle (5) and slidably engaged with the inner wall of the handle (5); The two ends of the gear (63) are respectively inserted into the two side walls of the handle (5) and are rotated together. A shift rod (64) is provided on the side of the gear (63) facing away from the rack (62). One end of the shift rod (64) is fixedly connected to the outer circular surface of the gear (63), and the other end penetrates the side wall of the handle (5) along the radial direction of the gear (63) and extends out of the handle (5).

8. The lung cancer tumor sampling device according to claim 7, characterized in that: The gear (63) is provided below the rack (62), a limiting groove (621) is provided on the top surface of the rack (62), a rotating bar (65) is provided above the rack (62), the middle portion of the rotating bar (65) is rotatably connected to the inner wall of the handle (5), and the rotation center line is parallel to the rotation center line of the gear (63); The bottom end of the rotating bar (65) extends obliquely downward toward the sampling slot (11) and contacts the top surface of the rack (62). The top end penetrates the top wall of the handle (5) and extends out of the handle (5). When the bottom end of the rotating bar (65) is inserted into the limiting slot (621), the angle between the rotating bar (65) and the rack (62) is acute.

9. A method for sampling lung cancer tumors, comprising using the device according to any one of claims 2 to 6, characterized in that: Here are the steps: S1. Preoperative localization: Determine the tumor location and puncture path through imaging examination; S2. Array puncture: multiple needle cores (1) are synchronously inserted into the tumor tissue, with the sampling grooves (11) of each needle core (1) located in different areas of the tumor; S3, step-by-step sampling: S31, pushing the push plate (4) through the adjustment part to move the needle core (1) forward, and the sampling groove (11) of each needle core (1) is moved out from the corresponding sleeve (2) to be exposed; S32, keeping the position of the cannula (2) fixed, pulling back the needle core (1) by the push plate (4), anchoring the tumor tissue by the barb on the front edge of the sampling groove (11), cooperating with the front end of the cannula (2) to shear the tumor tissue, and storing the sheared tumor tissue in the sampling groove (11); S4. Sample recovery: Pull out the needle core (1) and the cannula (2) as a whole, push the push plate (4) again through the adjustment part to move the needle core (1) forward, expose the sampling grooves (11) on each needle core (1), take out the samples in the multiple sampling grooves (11), and record them by number; S5. Heterogeneity analysis: molecular testing is performed on the samples obtained from each needle core (1).

10. The lung cancer tumor sampling method according to claim 9, characterized in that: The step S2 specifically includes: S21, rotating the two connecting bars (31) to adjust the relative position and fix them, for example, the two connecting bars (31) are collinear / parallel / the extension lines intersect, and the corresponding plurality of needle cores (1) are arranged in a straight line / in a matrix / in a radial arrangement; S22, inserting multiple needle cores (1) into the tumor tissue simultaneously, with the sampling grooves (11) of the needle cores (1) being located in different areas of the tumor.

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