Biological sample detection card box and biological sample detection method

Abstract

The invention relates to the technical field of biological sample detection equipment, and discloses a biological sample detection card box and a biological sample detection method. The biological sample detection card box comprises a box body assembly, a pipetting suction head and a plunger assembly, wherein the pipetting suction head and the plunger assembly are arranged on the box body assembly; the box body assembly comprises a bottom box, a packaging film, a detection box and an upper box rotationally matched with the bottom box, a sample storage area, a liquid storage area and a detection area are arranged at the end, close to the upper box, of the bottom box, a plurality of detection holes corresponding to the detection area are formed in the end, away from the upper box, of the bottom box, the packaging film seals the liquid storage area, and the detection box is arranged on the bottom box and corresponds to the detection area; the detection box is used for arranging at least two detection tubes for storing detection reagents, and the upper box is provided with a sample adding hole; the pipetting suction head is used for sucking and transferring liquid; the plunger assembly is used for sealing the detection pipe. The biological sample detection card box solves the problem of single detection index in the prior art, so that the practicability is high, and meanwhile, the detection efficiency and precision are improved.

Description

Technical Field

[0001] The present application relates to the technical field of biological sample detection equipment, and in particular, to a biological sample detection cartridge and a biological sample detection method. Background Art

[0002] Nucleic acids are the carriers of genetic information in living organisms and are essential components of all known life forms. Two types of nucleic acids, DNA and RNA, are primarily found within the cell nucleus, bound to proteins. With the rapid development of molecular biology, the research and analysis of nucleic acids has been increasingly promoted and applied in clinical diagnosis, food safety, environmental testing, agriculture, forestry, and animal husbandry.

[0003] Nucleic acid extraction is a very important part of life science research, biotechnology applications and genetic diagnosis. Early nucleic acid extraction mainly used phenol-chloroform extraction to remove impurities and ethanol precipitation of nucleic acids, but this method is cumbersome to operate. However, the nucleic acid extraction reagents currently on the market are mainly based on solid-phase adsorption methods, that is, nucleic acid molecules are adsorbed on the surface of a certain solid-phase adsorbent under specific conditions (such as pH, specific salts, etc.), and then eluted for purification. There are mainly two types: 1) Centrifugal column method: This method has high extraction efficiency and good extraction effect. It is currently widely used, but it requires repeated centrifugation, which is not conducive to high-throughput and automated operations; 2) Magnetic bead method: This method is based on magnetic adsorption and is easy to automate and operate in high-throughput, but the cost is high and the extraction efficiency is easily affected. Both of the above methods have the following problems: the operation is relatively cumbersome, and only one pathogen can be detected once at a time, and the detection indicators are relatively single. Summary of the Invention

[0004] The purpose of this application is to provide a biological sample detection cartridge and a biological sample detection method to solve the problem of single detection index in the prior art.

[0005] To achieve the above objectives, in a first aspect, the present application provides a biological sample detection cartridge, comprising:

[0006] The box body assembly includes a bottom box, a packaging film, a detection box, and an upper box rotatably matched with the bottom box, the bottom box having a sample storage area, a liquid storage area, and a detection area at one end close to the upper box, and a plurality of detection holes corresponding to the detection areas at one end of the bottom box away from the upper box. The packaging film covers the end of the bottom box close to the upper box for sealing the liquid storage area. The detection box is arranged in the bottom box and corresponds to the detection area. The detection box is used to set at least two detection tubes for storing detection reagents. The upper box has a sample addition hole for communicating with the sample storage area.

[0007] A pipette tip, disposed on the upper box, for aspirating and transferring liquid; and

[0008] The plunger assembly is movably arranged on the upper box and is used for sealing the detection tube.

[0009] As a further improvement of the above technical solution:

[0010] In combination with the first aspect, in a possible implementation manner, the detection box is detachably connected to the base box.

[0011] In combination with the first aspect, in a possible implementation manner, at least two positioning through holes are provided on the detection box, and the positioning through holes correspond to the detection holes and are used to accommodate the detection tube.

[0012] In combination with the first aspect, in one possible implementation, the plunger assembly includes:

[0013] A plunger rod is movably arranged on the upper box, and one end of the plunger rod away from the bottom box is flush with the other end of the upper box away from the bottom box;

[0014] A plunger frame, disposed at one end of the plunger rod close to the bottom box; and

[0015] A plurality of plunger heads are arranged on the plunger rack, and the plunger heads correspond to the detection tubes.

[0016] In combination with the first aspect, in a possible implementation manner, the box body assembly further includes a connecting hook, which penetrates and connects the upper box and the bottom box and can rotate relative to the bottom box.

[0017] In combination with the first aspect, in a possible implementation manner, a first limiting portion and a second limiting portion are respectively provided at both ends of the connecting hook, the first limiting portion is engaged with the upper box in a limiting manner, and the second limiting portion is engaged with the bottom box in a limiting manner;

[0018] Wherein, a concave-convex positioning structure is provided between the upper box and the first limiting portion.

[0019] In combination with the first aspect, in a possible implementation, the box assembly further includes a sample loading cover, the sample loading cover including a cover body and a sealing ring integrally connected to the cover body;

[0020] Wherein, the sealing ring is arranged between the connecting hook and the upper box, and the cover body is used to seal the sample addition hole.

[0021] In combination with the first aspect, in a possible implementation manner, a slot for accommodating the sealing ring is provided on the upper box, and limiting protrusions for limiting the sealing ring are provided on two opposite side walls of the slot.

[0022] To achieve the above objectives, in a second aspect, the present application further provides a biological sample detection method, which is applied to the biological sample detection cartridge provided according to the first aspect, wherein the sample storage area includes a sample cavity, and the liquid storage area includes a liquid storage cavity for separately storing an additive liquid. The biological sample detection method comprises:

[0023] S100: adding a sample into the sample chamber through the sample addition hole;

[0024] S200: heating the sample to a preset temperature;

[0025] S300: rotating the upper box according to the operating instruction so that the sample adding hole can be switched between the sample storage area and the liquid storage area, and the liquid is transferred to the sample chamber by piercing the packaging film with the pipette tip;

[0026] S400: Mixing, heating and magnetic adsorption of mixed samples;

[0027] S500: transferring the processed mixed sample into each test tube and mixing it evenly, and then sealing all the test tubes by the plunger assembly;

[0028] S600: Detect all the detection tubes simultaneously or sequentially through the detection holes.

[0029] As a further improvement of the above technical solution:

[0030] In conjunction with the second aspect, in a possible implementation, the liquid storage area further includes a cleaning chamber storing cleaning liquid, and before step S500, the following steps are further included:

[0031] The upper box is rotated according to the operating instructions, and the sample addition hole is made to correspond to the cleaning cavity, and the pipette tip is controlled to pass through the sample addition hole and pierce the packaging film to enter the cleaning cavity for washing.

[0032] Compared with the prior art, the present invention has the following advantages:

[0033] The present application provides a biological sample detection card box and a biological sample detection method, wherein the biological sample detection card box is provided with a sample storage area, a liquid storage area and a detection area on the bottom box. When in use, the liquid for detection is first added to the corresponding chamber in the liquid storage area, and then the packaging is sealed by a packaging film. At least two detection tubes are provided in the detection box, and each detection tube can store the same detection reagent or different detection reagents. Before the detection, the sample to be detected is first added to the sample storage area through the sample addition hole, and then the upper box is rotated and the liquid is sucked and transferred with a pipette tip to perform pre-detection treatment on the sample to be detected. Finally, the treated sample is added to the detection tube, and each detection tube is sealed by a plunger assembly. Then, each detection tube is detected simultaneously or sequentially. Therefore, the biological sample detection card box provided by the present application is compatible with liquid systems and freeze-drying systems, and is configured with a detection box. At least two detection tubes are provided in the detection box, and different detection tubes can store different or the same detection reagents. In this way, the same pathogen can be detected multiple times on the same sample at one time, or different pathogens can be detected at one time, which solves the problem of single detection indicators in the existing technology, thereby having strong practicality and improving the efficiency and accuracy of detection.

[0034] Furthermore, the biological sample detection card box provided in this application only requires manual sample addition once, and the entire process is carried out in a closed working environment, with high detection accuracy. At the same time, it reduces the requirements for professional personnel and dependence on standard laboratories. There is no need to match professional technicians and standard laboratory environments. It is suitable for immediate nucleic acid extraction and detection needs outside the laboratory, is conducive to immediate detection, is small in size, easy to carry, and simple to operate.

[0035] Other features and advantages of the present application will be described in detail in the subsequent detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the following detailed description, they are used to explain the present application. It should be understood that the following drawings only illustrate certain embodiments of the present application and should not be considered as limiting the scope. A person of ordinary skill in the art can also derive other relevant drawings based on these drawings without inventive effort. In the drawings:

[0037] Figure 1 The embodiment of the present application provides a front view of a biological sample detection cartridge;

[0038] Figure 2 Shown Figure 1 An exploded schematic diagram of the biological sample detection cartridge shown;

[0039] Figure 3 Shown Figure 2A top view of the bottom box in the biological sample detection cartridge shown;

[0040] Figure 4 Shown Figure 2 A schematic diagram of the three-dimensional structure of the bottom box in the biological sample detection card box shown;

[0041] Figure 5 Shown Figure 2 A schematic diagram of the three-dimensional structure of the detection box in the biological sample detection card box shown;

[0042] Figure 6 Shown Figure 2 A schematic diagram of the three-dimensional structure of the upper box in the biological sample detection card box shown;

[0043] Figure 7 Shown Figure 2 A schematic diagram of the three-dimensional structure of the plunger assembly in the biological sample detection cartridge shown;

[0044] Figure 8 Shown Figure 2 The three-dimensional structural diagram of the connecting hook in the biological sample detection cartridge is shown.

[0045] Description of reference numerals:

[0046] 100, box assembly; 101, center hole; 110, bottom box; 111, sample storage area; 1110, sample chamber; 112, liquid storage area; 1120, cleaning chamber; 1121, magnetic bead chamber; 113, detection area; 1130, detection guide hole; 114, detection hole; 115, detection chamber; 120, packaging film; 130, detection box; 131, detection tube; 1310, sealing film; 132 , positioning through hole; 140, upper box; 141, sample loading hole; 142, receiving hole; 143, piston hole; 144, sink; 145, card slot; 146, limiting protrusion; 147, positioning hole; 150, connecting hook; 151, first limiting portion; 152, second limiting portion; 160, sample loading cover; 161, cover body; 162, sealing ring; 163, flexible connector; 170, bottom sealing membrane;

[0047] 200, pipette tips;

[0048] 300, plunger assembly; 310, plunger rod; 320, plunger frame; 330, plunger head. DETAILED DESCRIPTION

[0049] The following describes the specific implementation of the embodiment of the present application in detail with reference to the accompanying drawings. It should be understood that the specific implementation described herein is only used to illustrate and explain the embodiment of the present application and is not intended to limit the embodiment of the present application.

[0050] It should be noted that, unless there is any conflict, the embodiments and features in the embodiments of this application can be combined with each other.

[0051] In the embodiments of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0052] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. Throughout the description of this application, "plurality" means two or more, unless otherwise specifically defined.

[0053] In this application, unless otherwise expressly specified or limited, terms such as "mounted," "connected," "connect," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components or interactions between two components. Those skilled in the art will understand the specific meanings of these terms in this application based on specific circumstances.

[0054] The present application will be described in detail below with reference to the accompanying drawings and in conjunction with exemplary embodiments.

[0055] Example

[0056] See also Figure 1 、 Figure 2 and Figure 3 This embodiment provides a biological sample detection cartridge that is compatible with both liquid and freeze-drying systems, is highly practical, and improves detection efficiency and accuracy.

[0057] The biological sample detection cartridge provided in this embodiment includes a cartridge assembly 100, a pipette tip 200, and a plunger assembly 300. The pipette tip 200 and the plunger assembly 300 are both disposed in the cartridge assembly 100. When in use, the pipette tip 200 can be removed by a pipette gun for aspirating and transferring liquid.

[0058] The box assembly 100 includes a base box 110, a packaging film 120, a detection box 130, and an upper box 140 that rotates with the base box 110. The end of the base box 110 near the upper box 140 is provided with a sample storage area 111, a liquid storage area 112, and a detection area 113. The end of the base box 110 away from the upper box 140 is provided with a plurality of detection holes 114 corresponding to the detection area 113. The packaging film 120 covers the end of the base box 110 near the upper box 140 and is used to seal the liquid storage area 112. The upper box 140 is provided with a sample loading hole 141 for communicating with the sample storage area 111. In this way, by rotating the upper box 140, the sample loading hole 141 can pass through the sample storage area 111, the liquid storage area 112, and the detection area 113 in sequence, allowing the pipette tip 200 to pass through the sample loading hole 141 and enter the corresponding sample storage area 111, the liquid storage area 112, and the detection area 113.

[0059] The detection box 130 is arranged in the bottom box 110 and corresponds to the detection area 113. The detection box 130 is used to set at least two detection tubes 131, and the detection tubes 131 are used to store detection reagents. In this way, it can be understood that each detection tube 131 has a corresponding detection hole 114, and the corresponding detection tube 131 can be detected through the corresponding detection hole 114. It should also be noted that the same detection reagent can be stored in different detection tubes 131, so that the same indicator can be tested multiple times at a time, improving the detection precision and accuracy. Of course, different detection reagents can be stored in different detection tubes 131, so that multiple different indicators can be tested at a time, improving detection efficiency, and effectively solving the problem of single detection in the existing technology.

[0060] Furthermore, the packaging film 120 is connected to the bottom box 110 through a thermal packaging process. Of course, in some embodiments, the packaging film 120 can also be connected to the bottom box 110 by gluing.

[0061] Please also refer to Figure 6 The upper case 140 also includes a receiving hole 142 for receiving a pipette tip 200. Initially, the pipette tip 200 is placed in the receiving hole 142, ready for removal for liquid transfer. The upper case 140 also includes a piston hole 143. A plunger assembly 300 is positioned within the piston hole 143 and is movable relative to the upper case 140. The plunger assembly 300 is used to seal the detection tube 131.

[0062] See also Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 and Figure 5Furthermore, the base box 110 is provided with a detection cavity 115 for accommodating the detection box 130, and the detection box 130 is detachably connected to the base box 110. This facilitates the placement and removal of detection tubes 131 in the detection box 130, thereby adjusting the type and quantity of detection tubes 131 as needed. Furthermore, detection boxes 130 of different capacities can be replaced according to testing requirements to ensure that the number of detection tubes 131 matches the testing needs.

[0063] Optionally, the detachable connection between the detection box 130 and the base box 110 includes a snap connection or a screw connection.

[0064] Furthermore, the detection box 130 is provided with at least two positioning holes 132, which correspond to the detection holes 114 and are used to accommodate the detection tube 131. It is understood that the number of positioning holes 132 can also be designed to be three, four, five, or another number. In this embodiment, the number of positioning holes 132 can be adjusted based on the size of the base box 110 and the detection requirements, and therefore is not limited in this embodiment.

[0065] It should be noted that when the detection tube 131 is inserted into the positioning through hole 132 , the detection tube 131 is pre-installed with a detection reagent, and the port of the detection tube 131 is sealed by a sealing film 1310 to avoid contamination.

[0066] In this embodiment, the sample storage area 111 includes a sample chamber 1110 for storing samples. The liquid storage area 112 includes multiple liquid storage chambers and at least one cleaning chamber 1120 for storing cleaning fluid. The detection area 113 includes multiple detection guide holes 1130, which connect to the detection chamber 115 and correspond in number to the positioning holes 132 on the detection box 130.

[0067] See also Figure 2 and Figure 7 The plunger assembly 300 includes a plunger rod 310, a plunger frame 320, and a plurality of plunger heads 330. The plunger rod 310 is movably mounted on the upper housing 140 and positioned within the piston hole 143 of the upper housing 140. The end of the plunger rod 310 distal from the bottom housing 110 is flush with the end of the upper housing 140 distal from the bottom housing 110. In this embodiment, the piston rod can move in the piston hole 143 under external force.

[0068] The plunger frame 320 is mounted on the end of the plunger rod 310 near the base housing 110. Multiple plunger heads 330 are mounted on the plunger frame 320. The plunger heads 330 correspond to the detection tube 131 and are used to seal the detection tube 131. Thus, when an external force drives the piston rod to move, the plunger frame 320 can drive the plunger heads 330 to move toward or away from the detection tube 131.

[0069] See also Figure 1 、 Figure 2 、 Figure 6 and Figure 8 In this embodiment, the box body assembly 100 further includes a connecting hook 150, which penetrates and connects the upper box 140 and the bottom box 110 and can rotate relative to the bottom box 110. Thus, the box body assembly 100 is provided with a central hole 101 that penetrates the upper box 140 and the bottom box 110, and the connecting hook 150 is located in the central hole 101, so that the upper box 140 can rotate relative to the bottom box 110 around the central hole 101.

[0070] The connecting hook 150 has a first stopper 151 and a second stopper 152 at both ends. The first stopper 151 is engaged with the upper box 140, while the second stopper 152 is engaged with the bottom box 110. Thus, the first stopper 151 and the second stopper 152 maintain contact between the bottom box 110 and the upper box 140, preventing them from separating.

[0071] Furthermore, a recess 144 for receiving the first limiting portion 151 is provided at one end of the upper box 140 away from the bottom box 110. When the first limiting portion 151 is received in the recess 144, the first limiting portion 151 is flush with the upper box 140, making the structure more compact and the appearance simpler.

[0072] The first stopper 151 is a plate-like structure, and a rotation-stopping fit is formed between the first stopper 151 and the recess 144. This prevents relative rotation between the first stopper 151 and the upper case 140, so that when the upper case 140 rotates, the entire connecting hook 150 also rotates. Furthermore, in this embodiment, a concave-convex positioning structure is provided between the upper case 140 and the first stopper 151 to facilitate positioning of the connecting hook 150 during installation, improving assembly efficiency.

[0073] The second limiting portion 152 is an umbrella-shaped buckle. A snap ring may be provided on the bottom case 110 to abut against the umbrella-shaped buckle. A clearance groove is provided on the end of the bottom case 110 away from the upper case 140 to allow passage of the connecting hook 150. The biological sample testing cartridge also includes a bottom sealing membrane 170 that seals the clearance groove. The sealing effect of the bottom sealing membrane 170 effectively prevents external contaminants from entering the cartridge assembly 100 through the assembly gap between the connecting hook 150 and the cartridge body assembly 100, potentially causing contamination or interfering with test accuracy, thereby improving overall sealing.

[0074] See also Figure 1 and Figure 2In this embodiment, the cartridge assembly 100 further includes a sample loading cover 160 for sealing the sample loading hole 141. The sample loading cover 160 includes a cover body 161 and a sealing ring 162 integrally connected to the cover body 161. Specifically, the sealing ring 162 and the cover body 161 are connected as a whole via a flexible connector 163.

[0075] The sealing ring 162 is disposed between the connecting hook 150 and the upper box 140, and the cover 161 is used to seal the sample injection hole 141. Thus, the sealing ring 162 not only connects the cover 161 to the upper box 140, but also seals the assembly gap between the connecting hook 150 and the upper box 140. This prevents external contaminants from entering the box assembly 100 through the assembly gap between the connecting hook 150 and the box assembly 100, potentially causing contamination or interfering with test accuracy, thereby improving overall sealing.

[0076] See also Figure 6 Furthermore, a groove 145 for accommodating the sealing ring 162 is provided on the bottom of the groove 144 of the upper box 140, and limiting protrusions 146 for limiting the sealing ring 162 are provided on the two opposite side walls of the groove 145. The limiting protrusions 146 are provided with multiple ones uniformly distributed around the axial direction of the groove 145. The sealing ring 162 can be better limited in the groove 145 through the limiting protrusions 146.

[0077] Optionally, the sealing ring 162 may be an O-ring, a trapezoidal ring, or the like.

[0078] See also Figures 1 to 8 When assembling the biological sample detection cartridge provided in this embodiment, first install the sealing ring 162 of the sample loading cap 160 and the connecting hook 150, then install it into the central hole 101 of the upper box 140, and then install the pipette tip 200 and the plunger assembly 300 into the upper box 140. When assembling the bottom box 110, first heat-seal the bottom sealing film 170 with the bottom box 110, then add the detection liquid to the corresponding liquid storage cavity in the liquid storage area 112 of the bottom box 110, and then heat-seal the packaging film 120 with the bottom box 110. The packaged bottom box 110 and the upper box 140 are connected as a whole through the connecting hook 150. Prepare the detection tube 131, add the detection reagent (pre-packaged liquid reagent / lyophilized ball) into the detection tube 131, and seal it with the sealing film 1310. The prepared detection tube 131 is installed into the corresponding positioning through hole 132 on the detection box 130, and then the detection box 130 is installed into the detection cavity 115 of the base box 110 for standby use. The assembly of the entire biological sample detection cartridge is completed.

[0079] See also Figures 1 to 8Furthermore, this embodiment also provides a biological sample detection method, which is applied to the biological sample detection cartridge provided above. The sample storage area 111 includes a sample cavity 1110, and the liquid storage area 112 includes a liquid storage cavity for storing a separate additive liquid. The biological sample detection method includes:

[0080] S100: Add sample to the sample chamber 1110 through the sample addition hole 141;

[0081] S200: heating the sample to a preset temperature;

[0082] S300: Rotate the upper box 140 according to the operating instructions so that the sample addition hole 141 can be switched between the sample storage area 111 and the liquid storage area 112, and use the pipette tip 200 to pierce the packaging film 120 to absorb the liquid and transfer it to the sample chamber 1110;

[0083] S400: Mixing, heating and magnetic adsorption of mixed samples;

[0084] S500: Transfer the processed mixed sample to each detection tube 131 and mix it evenly, and then seal all the detection tubes 131 using the plunger assembly 300;

[0085] S600 : Detect all the detection tubes 131 simultaneously or sequentially through the detection holes 114 .

[0086] The liquid storage area 112 further includes a cleaning chamber 1120 for storing cleaning liquid. Before step S500, the following steps are further included:

[0087] The upper box 140 is rotated according to the operating instructions, and the sample loading hole 141 is aligned with the cleaning chamber 1120 , and the pipette tip 200 is controlled to pass through the sample loading hole 141 and pierce the packaging film 120 to enter the cleaning chamber 1120 for washing.

[0088] In some embodiments, the plurality of liquid storage chambers may further include a magnetic bead chamber 1121, a lysis liquid chamber, an elution chamber, a first washing liquid chamber, a second washing liquid chamber, two cleaning chambers 1120 for storing pure water, and a protease chamber. The arrangement order of the above-mentioned liquid storage chambers can be adjusted according to actual use requirements.

[0089] When using the biological sample detection cartridge for nucleic acid detection, the cover 161 of the sample loading cover 160 can be opened first, and the sample can be added to the sample chamber 1110 through the sample loading hole 141. Then, the cover 161 is closed, and the biological sample detection cartridge is placed in the biological sample detection device. The sample in the sample chamber 1110 is heated to 65°, and the driving member is inserted into the two oppositely arranged positioning holes 147 of the upper box 140 for positioning; then the pipette gun takes out the pipette tip 200 and rises a certain distance; then the upper box 140 is driven to rotate a certain angle against the bottom box 110 so that the sample loading hole 141 is aligned with the magnetic bead chamber 1121, and the pipette tip 200 is driven downward to pierce the packaging film 120, and the liquid in the magnetic bead chamber 1121 is taken out and transferred to the sample chamber 1110; then the liquid in the protease chamber and the lysate chamber is rotated in sequence and added to the sample chamber 1110 and blown evenly. After heating for a period of time, the magnet is close to the side wall of the sample chamber 1110. After adsorption for a period of time, the adsorbed liquid is transferred to the lysis liquid chamber, and the upper box 140 is driven to rotate again to transfer the liquids in the first washing liquid chamber and the second washing liquid chamber to the sample chamber 1110 in turn for magnet adsorption, and then put them back into their respective chambers in turn; then the heating is started again, the sample chamber 1110 is dried and volatilized for a preset time, and then the upper box 140 is rotated, and the pipette tip 200 is aligned with the washing chamber 1120 for up and down washing; after the end, the upper box 140 is rotated, the pipette tip 200 is transferred to the top of the elution chamber, and then it is lowered to puncture and take out the liquid into the sample chamber 1110. The magnet is lowered to blow and mix the liquid to ensure that the eluent and magnetic beads are evenly mixed; then the magnet is adsorbed, and after the preset time, the clarified liquid is quantitatively transferred to each detection tube 131 and blown and mixed, and then the preset angle is rotated to reset the pipette tip 200, and the plunger assembly 300 is driven to press down and seal the detection tube 131.

[0090] At this point, the entire sample pre-treatment and PCR reaction system is completed, and fluorescence detection is directly performed through the detection hole 114 through the heating and fluorescence collection module.

[0091] It should be noted that, since the detection reagent is pre-packaged in the detection tube 131 in this embodiment, steps such as enzyme catalysis and internal standardization can be omitted.

[0092] Therefore, the biological sample detection card box provided in this embodiment is compatible with liquid systems and freeze-drying systems, and is configured with a detection box 130. At least two detection tubes 131 are set in the detection box 130. Different detection tubes 131 can store different or the same detection reagents. In this way, multiple tests of the same pathogen can be performed on the same sample at one time, or different pathogens can be detected at one time. It is highly practical and improves the efficiency and accuracy of the detection.

[0093] Furthermore, the biological sample detection cartridge provided in this embodiment only requires manual sample addition once, and the entire process is carried out in a closed working environment, with high detection accuracy. At the same time, it reduces the requirements for professional personnel and dependence on standard laboratories. There is no need to match professional technicians and standard laboratory environments. It is suitable for the needs of immediate nucleic acid extraction and detection outside the laboratory, is conducive to immediate detection, is small in size, easy to carry, and simple to operate.

[0094] The above describes in detail the optional implementation methods of the embodiments of the present application in conjunction with the accompanying drawings. However, the embodiments of the present application are not limited to the specific details of the above implementation methods. Within the technical concept of the embodiments of the present application, various simple modifications can be made to the technical solutions of the embodiments of the present application, and these simple modifications all fall within the scope of protection of the embodiments of the present application.

[0095] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner unless there is any contradiction. In order to avoid unnecessary repetition, the embodiments of this application will no longer separately describe various possible combinations.

[0096] In addition, the various implementation methods of the embodiments of the present application can be arbitrarily combined, as long as they do not violate the ideas of the embodiments of the present application, they should also be regarded as the contents disclosed in the embodiments of the present application.

Claims

1. A biological sample detection cartridge, characterized in that: include: The box body assembly (100) comprises a bottom box (110), a packaging film (120), a detection box (130) and an upper box (140) rotatably matched with the bottom box (110), wherein the end of the bottom box (110) close to the upper box (140) is provided with a sample storage area (111), a liquid storage area (112) and a detection area (113), and the end of the bottom box (110) away from the upper box (140) is provided with a plurality of detection holes (114) corresponding to the detection area (113). The membrane (120) covers one end of the bottom box (110) close to the upper box (140) and is used to seal the liquid storage area (112). The detection box (130) is arranged on the bottom box (110) and corresponds to the detection area (113). The detection box (130) is used to set at least two detection tubes (131), and the detection tubes (131) are used to store detection reagents. The upper box (140) is provided with a sample addition hole (141) for communicating with the sample storage area (111); a pipette tip (200), disposed on the upper box (140), for sucking and transferring liquid; and The plunger assembly (300) is movably arranged on the upper box (140) and is used to seal the detection tube (131).

2. The biological sample detection cartridge according to claim 1, wherein: The detection box (130) is detachably connected to the base box (110).

3. The biological sample detection cartridge according to claim 1, wherein: The detection box (130) is provided with at least two positioning through holes (132), and the positioning through holes (132) correspond to the detection holes (114) and are used to accommodate the detection tube (131).

4. The biological sample detection cartridge according to claim 1, wherein: The plunger assembly (300) comprises: A plunger rod (310) is movably arranged on the upper box (140), and one end of the plunger rod (310) away from the bottom box (110) is flush with one end of the upper box (140) away from the bottom box (110); A plunger frame (320) is provided at one end of the plunger rod (310) close to the bottom box (110); and A plurality of plunger heads (330) are arranged on the plunger rack (320), and the plunger heads (330) correspond to the detection tubes (131).

5. The biological sample detection cartridge according to claim 1, wherein: The box body assembly (100) further comprises a connecting hook (150), wherein the connecting hook (150) penetrates and connects the upper box (140) and the bottom box (110), and is rotatable relative to the bottom box (110).

6. The biological sample detection cartridge according to claim 5, characterized in that: The two ends of the connecting hook (150) are respectively provided with a first limiting portion (151) and a second limiting portion (152), the first limiting portion (151) is in limiting cooperation with the upper box (140), and the second limiting portion (152) is in limiting cooperation with the bottom box (110); Wherein, a concave-convex positioning structure is provided between the upper box (140) and the first limiting portion (151).

7. The biological sample detection cartridge according to claim 5, characterized in that: The box assembly (100) further includes a sample loading cover (160), wherein the sample loading cover (160) includes a cover body (161) and a sealing ring (162) integrally connected to the cover body (161); The sealing ring (162) is provided between the connecting hook (150) and the upper box (140), and the cover (161) is used to seal the sample addition hole (141).

8. The biological sample detection cartridge according to claim 7, wherein: The upper box (140) is provided with a slot (145) for accommodating the sealing ring (162), and two opposite side walls of the slot (145) are provided with limiting protrusions (146) for limiting the sealing ring (162).

9. A biological sample detection method, characterized in that: Applicable to a biological sample detection cartridge according to any one of claims 1 to 8, the sample storage area (111) includes a sample cavity (1110), the liquid storage area (112) includes a liquid storage cavity for separately storing an additive liquid, and the biological sample detection method includes: S100: adding a sample into the sample chamber (1110) through the sample addition hole (141); S200: heating the sample to a preset temperature; S300: rotating the upper box (140) according to the operating instructions so that the sample addition hole (141) can be switched between the sample storage area (111) and the liquid storage area (112), and piercing the packaging film (120) with the pipette tip (200) to absorb the liquid and transfer it to the sample chamber (1110); S400: Mixing, heating and magnetic adsorption of mixed samples; S500: transferring the processed mixed sample into each detection tube (131) and mixing it evenly, and then sealing all the detection tubes (131) by the plunger assembly (300); S600: Detect all the detection tubes (131) simultaneously or sequentially through the detection hole (114).

10. The biological sample detection method according to claim 9, characterized in that: The liquid storage area (112) further includes a cleaning chamber (1120) storing cleaning liquid, and before step S500, the following steps are further included: The upper box (140) is rotated according to the operating instructions so that the sample loading hole (141) corresponds to the cleaning chamber (1120), and the pipette tip (200) is controlled to pass through the sample loading hole (141) and pierce the packaging film (120) to enter the cleaning chamber (1120) for washing.

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