A method and apparatus for measuring the three-dimensional distribution of a food-medicine homology composition.

Abstract

This invention discloses a method and apparatus for measuring the three-dimensional distribution of a medicinal and edible homology composition, belonging to the field of plant detection technology. The invention uses laser ablation to vaporize a slice of the composition, generating gas molecules while preserving the molecular structure of the target component. The vaporization completion time is determined based on first spectral data, reducing impurities in the gas molecules. Then, the gas molecules are ionized in an ionization chamber to generate sample ions, improving ionization efficiency, increasing ion signal intensity, and minimizing interference. The target ions and other ions in the sample ions are separated using a mobility chamber and a flight channel, improving detection accuracy. Furthermore, based on the two-dimensional data, thickness, and target component content of the composition slice, multiple three-dimensional slice models are generated to establish a three-dimensional content model of the analyte, clearly displaying the content and uniformity of the target component in the analyte.

Description

Technical Field

[0001] This invention relates to the field of plant detection technology, and in particular to a method and apparatus for measuring the three-dimensional distribution of a medicinal and edible homologous composition. Background Technology

[0002] Hawthorn, as a medicinal and edible herb, possesses various effects and functions, and can be used to prepare medicinal and edible compositions. Quercetin is one of the key active ingredients in hawthorn, possessing antioxidant, anti-inflammatory, and cardiovascular protective effects. Substances in hawthorn with similar mass-to-charge ratios to quercetin, such as kaempferol and isorhamnetin, require differentiation to determine the quercetin content. Chinese patent application CN120314418A discloses a method for determining mangiferin in mango extract. This method first ionizes sample ions from a target material, with at least a portion of the sample ions entering a mobility cell. After capturing the sample ions, mass spectrometry data is generated, and first and second sampling data are extracted from the mass spectrometry data. This invention directly ionizes sample ions from the target material, resulting in excessive impurities and a high degree of ion fragmentation, which is detrimental to the subsequent separation of the target analyte, substances with similar mass-to-charge ratios, and isomers based on the mobility cell, thus reducing detection accuracy. Food and medicine homology compositions typically exist in solid forms such as extracts and pills. Due to the uneven mixing of raw materials, multiple slicing operations are required to obtain a three-dimensional content distribution when detecting the active ingredients in these compositions, in order to more accurately determine the content of each active ingredient. Therefore, there is a need for further improvement in existing technologies. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention proposes a method and apparatus for measuring the three-dimensional distribution of food-medicine homology compositions. First, the analyte is sliced ​​to obtain multiple slices. These slices are then vaporized using laser ablation to generate gas molecules. The vaporization completion time is determined based on first spectral data to reduce impurities in the gas molecules. Next, the gas molecules are ionized in an ionization chamber to generate sample ions. Target ions and impurity ions are separated from the sample ions using a mobility chamber and a flight channel to obtain the content of the target component, thus improving detection accuracy. Furthermore, based on the two-dimensional data, thickness, and target component content of the slices, multiple three-dimensional slice models are generated, thereby establishing a three-dimensional content model of the analyte. This clearly displays the content and uniformity of the target component in the analyte, expanding its application scope and facilitating data analysis.

[0004] The technical solution of this invention is implemented as follows:

[0005] A method for measuring the three-dimensional distribution of a food-medicine homology composition includes the following steps:

[0006] Step 1: Place the sample to be tested on the experimental stage, and the first light emitter vertically aligns with the sample to be tested on the experimental stage to emit the first laser.

[0007] Step 2: The sample to be tested generates gas molecules. The optical transceiver emits a second laser to the gas molecules to generate the first spectral data. Based on the first spectral data, the first optical emitter and the optical transceiver are turned off, and the first moment is recorded.

[0008] Step 3: Open the first and second valves to introduce the carrier gas. The gas molecules enter the ionization chamber, and the ionization chamber generates an electrospray to ionize the gas molecules and generate sample ions. Open the third valve to introduce the sample ions into the mobility chamber and record the second time.

[0009] Step 4: Sample ions pass through the mobility chamber and enter the flight channel. After the mass analyzer captures the sample ions, it generates the first ion mobility spectrum and extracts the sampling data.

[0010] Step 5: Repeat steps 1 to 4 to obtain the sampling dataset, optimize the ablation parameters, ionization parameters, and mobility parameters, obtain the standard curve of the target component, prepare multiple composite slices of the analyte, and place the composite slices on the experimental table in the order of slices.

[0011] Step 6: Obtain the two-dimensional data and thickness of the composition slices, and divide the target mesh;

[0012] Step 7: Move the first light emitter and light transceiver according to the target grid, repeat steps 1 to 4, obtain the second ion mobility spectrum, and calculate the content of the target component in the target grid;

[0013] Step 8: If the target grid has been completely traversed, determine whether the composition slice has been completely traversed. If yes, proceed to step 9; otherwise, place the next composition slice on the experimental table and return to step 6. Otherwise, proceed to the next target grid and return to step 7.

[0014] Step 9: Based on the two-dimensional data, thickness, and content of target components in multiple target grids of the composition slices, generate multiple three-dimensional slice models to establish a three-dimensional content model of the analyte to be detected.

[0015] In this invention, in step 1, the experimental stage includes a glass cover and a sample stage. The glass cover maintains a vacuum environment. The sample to be tested is a thin sheet. The sample to be tested is placed on the sample stage. The laser energy is initially set to a reference energy.

[0016] In this invention, in step 2, the optical transceiver includes a second optical transmitter and a signal acquisition device. The signal acquisition device is equipped with a bandpass filter. The signal acquisition device receives fluorescence signals and generates first spectral data. The first spectral data is fluorescence spectral data. When the fluorescence signal drops to the baseline, the first optical transmitter and the optical transceiver are turned off. The first moment is the time when the first optical transmitter and the optical transceiver are turned off.

[0017] In this invention, in step 3, the first valve controls the carrier gas to enter the experimental stage, the second valve controls the gas molecules to enter the ionization chamber, and the third valve controls the sample ions to enter the mobility chamber. The carrier gas carries the gas molecules into the ionization chamber, and the sample ions are introduced into the mobility chamber through the ion funnel. The carrier gas is helium, the ionization chamber is in negative ion mode, and the electrospray contains methanol, water, and formic acid. The mass fraction of formic acid is 0.1%, and the volume ratio of methanol to water is 70%:30%. The second moment is the moment when the third valve is opened.

[0018] In this invention, in step 4, a first migration interval is calculated based on the first property of the target component and the buffer gas, a first flight interval is calculated based on the second property of the target component and the buffer gas, a sampling interval of the target component is calculated based on the first migration interval and the first flight interval, and the minimum sampling time and the maximum sampling time of the target component are obtained by combining the second time and the sampling interval, and sampling data is extracted from the first ion mobility spectrum based on the minimum sampling time and the maximum sampling time.

[0019] In this invention, in step 4, the mobility chamber contains a buffer gas, which is helium; the flight channel is a high vacuum environment; the sampling data is the target ion peak; the target component is quercetin; the first attribute is the ion collision cross section; and the second attribute is the mass-to-charge ratio.

[0020] In this invention, in step 5, the sampling dataset contains multiple sampling data with different parameters. An algorithm parameter set is obtained based on the sampling dataset. The algorithm parameter set includes a symmetry factor and peak width. An objective function is established based on the algorithm parameter set. The erosion parameters, ionization parameters, and mobility parameters are optimized based on the objective function and the optimization algorithm to obtain the target erosion parameters, target ionization parameters, and target mobility parameters. The substance to be detected is a food-medicine homology composition.

[0021] In this invention, in step 7, the center of the light spot of the first light emitter is aligned with the center of the target grid, the first laser is perpendicular to the experimental stage, the optical transceiver is parallel to the experimental stage, the second laser is not on the same plane as the first laser, the minimum sampling time and the maximum sampling time are recalculated, and the target ion peak of the second ion mobility spectrum is extracted. The content of the target component in the target grid is calculated according to the standard curve and the second ion mobility spectrum.

[0022] In this invention, in step 9, the target grid is aligned with the two-dimensional data of the composition slice, the content of the target component in the corresponding target grid is filled into the two-dimensional data, the two-dimensional data is widened according to the thickness to generate a three-dimensional slice model, and multiple three-dimensional slice models are stacked in sequence to establish a three-dimensional content model of the analyte.

[0023] A measuring device for measuring the three-dimensional distribution of the aforementioned food-medicine homology composition, comprising:

[0024] An experimental setup includes a glass cover and a sample stage. The glass cover maintains a vacuum environment, and the sample stage holds the sample to be tested and slices of the composition.

[0025] A first light emitter, which is used to emit a first laser;

[0026] An optical transceiver includes a second optical transmitter and a signal acquisition device. The second optical transmitter is used to emit a second laser, and the signal acquisition device is used to acquire fluorescence signals to generate first spectral data.

[0027] Ionization chamber, which is used to generate electrospray to ionize gas molecules and produce sample ions;

[0028] The mobility chamber is used to distinguish the target ion from other ions with similar mass-to-charge ratios;

[0029] The flight channel is used to separate the target ion and other ions with different mass-to-charge ratios in the sample ions through free flight in a high vacuum environment;

[0030] The mass analyzer is used to capture sample ions and generate a first ion mobility spectrum and a second ion mobility spectrum.

[0031] The data processing device is used to calculate the minimum sampling time and the maximum sampling time, extract the target ion peak from the second ion mobility spectrum, obtain the content of the target component in the target grid according to the standard curve, and establish a three-dimensional content model of the analyte.

[0032] The data analysis device is used to analyze the first moment based on the first spectral data, obtain the algorithm parameter set based on the sampling dataset, establish the objective function based on the algorithm parameter set, and optimize the ablation parameters, ionization parameters, and mobility parameters based on the objective function and the optimization algorithm.

[0033] A three-dimensional positioning system is used to acquire two-dimensional data and thickness of the composition slice, divide the target grid, move the first light emitter and light transceiver according to the target grid, and replace the composition slice to complete the measurement.

[0034] The method and apparatus for measuring the three-dimensional distribution of a food-medicine homology composition according to the present invention have the following beneficial effects: Gas molecules are generated by vaporizing the composition slices through laser ablation, preserving the molecular structure of the target component. The completion time of vaporization is determined based on the first spectral data. Then, the gas molecules are ionized in an ionization chamber to generate sample ions, reducing impurities in the gas molecules, improving ionization efficiency, increasing ion signal intensity, and minimizing interference. The target ions and other ions in the sample ions are separated using a mobility chamber and a flight channel, improving detection accuracy. Furthermore, multiple three-dimensional slice models are generated based on the two-dimensional data, thickness, and target component content of the composition slices, establishing a three-dimensional content model of the analyte, clearly displaying the content and uniformity of the target component in the analyte. Attached Figure Description

[0035] Figure 1 This is a flowchart of the method for measuring the three-dimensional distribution of the food-medicine homology composition of the present invention;

[0036] Figure 2 This is a schematic diagram of the experimental platform of the present invention;

[0037] Figure 3 This is a schematic diagram of the target ion peak in the ion mobility spectrum of this invention;

[0038] Figure 4 This is a schematic diagram illustrating the target mesh division of the present invention;

[0039] Figure 5 This is a schematic diagram of the first laser and the second laser of the present invention;

[0040] Figure 6 A schematic diagram illustrating the establishment of a three-dimensional content model for this invention;

[0041] Figure 7 This is a schematic diagram illustrating the principle of the method for measuring the three-dimensional distribution of the food-medicine homology composition of the present invention.

[0042] Figure 8 This is a block diagram of the apparatus for measuring the three-dimensional distribution of the food-medicine homology composition of the present invention.

[0043] The reference numerals in the attached figures are: ionization channel 100, first pipe 101, second pipe 102, first collimation channel 200, mobility pool 300, inlet gate 301, outlet gate 302, third pipe 303, second collimation channel 400, flight channel 500, repulsion zone 501, acceleration zone 502, field-free zone 503, first reflection zone 504, second reflection zone 505, exhaust pipe 506, and mass analyzer 600. Detailed Implementation

[0044] To better understand the purpose, technical solution, and advantages of this application, the application is described and illustrated below in conjunction with the accompanying drawings and embodiments.

[0045] Direct ionization leads to numerous fragmented ions and impurities in the target component of the analyte, resulting in many ion peaks and low ion signal intensity detected by the mass analyzer, thus reducing detection accuracy and prolonging detection time. Laser ablation of the analyte slices generates gas molecules that preserve the molecular structure of the target component (avoiding pyrolysis or fragmentation). The intact molecular structure of the target component (quercetin) combined with the electrospray droplets results in higher ionization efficiency, more readily forming quasi-molecular ions (such as the target ion with a mass-to-charge ratio of 301.03), higher ion signal intensity with less interference, a cleaner ion mobility spectrum, avoidance of overlapping peaks, and easier detection.

[0046] Among the analytes, substances with a mass-to-charge ratio similar to quercetin mainly include flavonoid isomers (such as kaempferol and isorhamnetin) and flavonoid glycoside dissociation products. Because the target ion of quercetin (an ion with a mass-to-charge ratio of 301.03) has a different structure and collision area than the characteristic ion of the flavonoid isomers, the mobility chamber uses an applied electric field to cause different migration velocities of different ions in the sample when colliding with the buffer gas, thus separating the target ion. This ensures that the target ion and the characteristic ion of the flavonoid isomer reach the mass analyzer at different times, preventing the target ion peak in the first ion mobility spectrum from overlapping with the peaks of other ions, thereby improving detection accuracy. The flight channel is a high-vacuum environment. Due to the different mass-to-charge ratios of the target ion and other ions, the target ion and other ions in the sample are separated through free flight under high vacuum. Example 1

[0047] like Figures 1 to 6 As shown, the method for measuring the three-dimensional distribution of the food-medicine homology composition of the present invention includes the following steps.

[0048] Step 1: Place the sample to be tested on the experimental stage, and the first light emitter vertically aligns with the sample on the experimental stage to emit the first laser beam. For example... Figure 2 As shown, the experimental setup includes a glass cover and a sample stage. A vacuum environment is maintained inside the glass cover, and the sample to be tested is placed on the sample stage. The sample to be tested is a thin sheet. The first light emitter can be an ultraviolet nanosecond laser with a wavelength of 266 nm. The initial laser energy is set to a reference energy, which can be set to 0.1 mJ / pulse. The reference energy cannot be too high, as this can easily lead to excessively rapid heating, pyrolysis of the target component, premature disappearance of the fluorescence signal, and simultaneous pyrolysis of impurities such as sugars, pigments, and pectin in the sample to be tested, producing volatile organic compounds. In this embodiment, the target component is quercetin.

[0049] Step 2: The sample to be tested generates gas molecules. The optical transceiver emits a second laser beam towards the gas molecules, generating first spectral data. Based on the first spectral data, the first optical emitter and the optical transceiver are turned off, and the first moment is recorded. The optical transceiver includes a second optical emitter and a signal acquisition device. The wavelength of the second laser is 370 nm, and the signal acquisition device is equipped with a bandpass filter of 500 nm to 550 nm. The target component (quercetin) generates a fluorescence signal (fluorescence peak at 520 nm) under the induction of the second laser. This fluorescence signal passes through the bandpass filter, shielding the fluorescence emitted by the pyrolysis products (such as impurities like pectin) and the laser-scattered light. The signal acquisition device receives the fluorescence signal and generates the first spectral data, which is the fluorescence spectrum data. When the fluorescence signal drops to the baseline, the first optical emitter and the optical transceiver are turned off. The first moment is the turning-off moment of the first optical emitter and the optical transceiver. Since the vaporization temperature of the target component is approximately 200℃-280℃ and the decomposition temperature is 250℃-350℃, the temperature is controlled at 200℃-250℃ by adjusting the laser energy of the first light emitter, thus preferentially vaporizing the target component. Meanwhile, impurities such as sugars remain due to carbonization. The time required for the target component to completely vaporize in the composition slice is analyzed based on the first spectral data, thereby reducing the amount of impurity gas entering the ionization chamber.

[0050] Step 3: Open the first and second valves to introduce carrier gas. Gas molecules enter the ionization chamber, where electrospray ionizes the gas molecules to produce sample ions. Open the third valve to introduce the sample ions into the mobility chamber and record the second time point. Figure 2 As shown, the glass cover includes a first valve and a second valve. The first valve controls the flow of carrier gas into the experimental stage, and the second valve controls the flow of gas molecules into the ionization chamber. The carrier gas carries the gas molecules into the ionization chamber, and then the sample ions are introduced into the mobility chamber through an ion funnel. The carrier gas is helium, and the ionization chamber operates in negative ion mode. The electrospray contains methanol, water, and formic acid, with a formic acid mass fraction of 0.1% and a methanol to water volume ratio of 70%:30%. A third valve controls the flow of sample ions into the mobility chamber; the second time point is the opening time of the third valve.

[0051] Step 4: Sample ions pass through the mobility chamber and enter the flight channel. After the mass analyzer captures the sample ions, it generates a first ion mobility spectrum and extracts the sampling data. The mobility chamber contains a buffer gas, namely helium. Sample ions are collected through an ion funnel and the carrier gas is filtered before being introduced into the mobility chamber to avoid affecting the mobility pressure of the mobility chamber. A first migration interval is calculated based on the target component and the first property of the buffer gas. A first flight interval is calculated based on the target component and the second property of the buffer gas. The sampling interval of the target component is calculated based on the first migration interval and the first flight interval. Then, the minimum sampling time and the maximum sampling time of the target component are obtained by combining the second time and the sampling interval. Sampling data is extracted from the first ion mobility spectrum based on the minimum and maximum sampling times. The flight channel is a high-vacuum environment, and the sampling data is the target ion peak. The first property is the ion collision cross section, and the second property is the mass-to-charge ratio.

[0052] Step 5: Repeat steps 1 to 4 to obtain the sampling dataset, optimize the ablation parameters, ionization parameters, and mobility parameters, obtain the standard curve of the target component, prepare multiple composite slices of the analyte, and place the composite slices on the experimental stage according to the slice order. The ablation parameters include the energy and spot size of the first laser. The ionization parameters include the ionization voltage. The mobility parameters include the electric field gradient and electric field strength. The flight parameters include the acceleration voltage, flight path length, and pulse frequency. The sampling dataset contains sampling data under multiple different parameters. Based on the sampling dataset, obtain the algorithm parameter set, which includes the symmetry factor and peak width. Establish an objective function based on the algorithm parameter set, and optimize the ablation parameters, ionization parameters, and mobility parameters based on the objective function and the optimization algorithm to obtain the target ablation parameters, target ionization parameters, and target mobility parameters. The optimization algorithm can be a particle swarm optimization algorithm, an ant colony optimization algorithm, etc.

[0053] like Figure 3 As shown, the horizontal axis represents time, and the vertical axis represents ion signal intensity. The symmetry factor is the peak shape symmetry of the target ion peak in the ion mobility spectrum, reflecting the transport efficiency of sample ions. Its value is equal to the ratio of the peak front width (the horizontal distance from 5% to 50% of the peak height) to the peak back width (the horizontal distance from 50% of the peak height to 95% of the peak back). The peak width is the width of the target ion peak in the ion mobility spectrum, usually expressed as the full width at half maximum (FWHM), that is, the width of the target ion peak at half its height. The narrower the peak width, the better the target ion (quercetin produces ions with a mass-to-charge ratio of 301.03 in negative ion mode), the less collision loss, and the higher the resolution.

[0054] Step 6: Obtain the two-dimensional data and thickness of the composition slices, and divide the target mesh. For example... Figure 4As shown, two-dimensional data of the composition slice is obtained by scanning with a three-dimensional positioning system. The slice center is generated based on the two-dimensional data of the composition slice. The grid size is adjusted according to the spot size of the first laser. The target grid is divided through the slice center and displayed through a low-power LED grid, reducing the impact on vaporization and detection.

[0055] Step 7: Move the first light emitter and transceiver according to the target grid, repeat steps 1 to 4, obtain the second ion mobility spectrum, and calculate the content of the target component in the target grid. For example... Figure 5 As shown, both the first light emitter and the optical transceiver are located outside the glass enclosure. The center of the light spot of the first light emitter is aligned with the center of the target grid. The first laser is perpendicular to the experimental stage, and the optical transceiver is parallel to the experimental stage. The second laser is not on the same plane as the first laser to avoid affecting the vaporization process of the target component. The minimum and maximum sampling times are recalculated, and the target ion peak of the second ion mobility spectrum is extracted. The standard curve is the relationship between the content of the target component and the intensity of the target ion peak under the target ablation parameters, target ionization parameters, and target mobility parameters.

[0056] Step 8: If the target grid has been completely traversed, determine whether the composition slices have been completely traversed. If yes, proceed to step 9; otherwise, place the next composition slice on the experimental platform and return to step 6. Otherwise, proceed to the next target grid and return to step 7.

[0057] Step 9: Based on the two-dimensional data, thickness, and content of the target component in multiple target grids of the composition slices, generate multiple three-dimensional slice models to establish a three-dimensional content model of the analyte. For example... Figure 6 As shown, the target grid is aligned with the two-dimensional data of the composition slice according to the fill arrows, and the content of the target component in the corresponding target grid is filled into the two-dimensional data (x, y, S). The three-dimensional slice model (x, y, D) is generated by widening the two-dimensional data according to the thickness. i Based on the center of each composite slice, align the three-dimensional slice model with the three-dimensional slice model, and stack multiple three-dimensional slice models in sequence to establish a three-dimensional content model (x, y, D, S) of the analyte. x is the abscissa of the target grid, y is the ordinate of the target grid, S is the content of the target component, and D... i Let be the thickness of slice i of the composition, and D be the thickness of the analyte. The three-dimensional content model of the analyte clearly displays the content and uniformity of the target component. Example 2

[0058] like Figure 7 As shown in the figure, this embodiment further discloses the principle of the method for measuring the three-dimensional distribution of food-medicine homology compositions.

[0059] The carrier gas flows out from the second valve port at a predetermined flow rate (1 L / min) and enters the ionization chamber 100 through the first pipe 101, heating the first pipe 101 to a predetermined temperature to prevent gas molecules from condensing. Electrospray enters the ionization chamber 100 through the second pipe 102, generating an electrospray to ionize the gas molecules and produce sample ions. The ionized sample ions are collected through an ion funnel and filtered by the carrier gas before entering the first collimation channel 200. Buffer gas enters the mobility cell 300 through the third pipe 103. The mobility cell 300 has an inlet gate 301 and an outlet gate 302. When the inlet gate 301 and outlet gate 302 are open, sample ions can freely enter and exit. The first collimation channel 200 modulates the sample ions into a first ion beam. After the outlet gate 302 is opened, at least a portion of the sample ions enter the second collimation channel 400, which modulates the sample ions into a second ion beam and guides the second ion beam into the flight channel 500. To reduce other interference, an exhaust duct 506 may be installed on the side wall of the flight passage 500 to reduce the internal air pressure.

[0060] When the second ion beam enters the flight channel 500, its vertical velocity is first accelerated by the repulsion zone 501 and the acceleration zone 502. When the second ion beam enters the field-free zone 503, it maintains a constant speed. Then, under the action of the first reflection zone 504 and the second reflection zone 505, it first decelerates to 0 and then accelerates in the opposite direction. When it re-enters the field-free zone 503, it maintains a constant speed until the second ion beam falls onto the mass analyzer 600. However, its horizontal velocity remains constant throughout the flight. Example 3

[0061] like Figure 8As shown, a measuring device for measuring the three-dimensional distribution of a medicinal and edible homology composition includes: an experimental stage, a first light emitter, a light transceiver, an ionization chamber, a mobility chamber, an ion funnel, a flight channel, a mass analyzer, a three-dimensional positioning system, a data processing device, and a data analysis device. The experimental stage includes a glass cover and a sample stage. The glass cover maintains a vacuum environment, and the sample stage holds the sample to be tested and slices of the composition. The first light emitter emits a first laser to ablate the composition slices and the sample to be tested, vaporizing them and generating gas molecules. The light transceiver includes a second light emitter and a signal acquisition device. The second light emitter emits a second laser to the gas molecules, and the signal acquisition device acquires fluorescence signals to generate first spectral data. The ionization chamber generates an electrospray to ionize the gas molecules, producing sample ions. The mobility chamber distinguishes the target ion from other ions with similar mass-to-charge ratios. The ion funnel is connected to the ionization chamber and the mobility chamber, filtering impurity gases and introducing sample ions from the ionization chamber into the mobility chamber. The flight channel is used to separate target ions and other ions with different mass-to-charge ratios from sample ions through free flight in a high-vacuum environment. The mass analyzer captures sample ions and generates a first and second ion mobility spectrum. The three-dimensional positioning system acquires two-dimensional data and thickness of the composition slice, divides the target grid, moves the first light emitter and transceiver according to the target grid, replaces the composition slice, and completes the measurement.

[0062] The data processing unit calculates the minimum and maximum sampling times, extracts sampling data from the second ion mobility spectrum based on these times, obtains the content of the target component in the target grid according to the standard curve, and establishes a three-dimensional content model of the analyte. The data analysis unit analyzes the first moment based on the first spectral data, obtains the algorithm parameter set based on the sampling dataset, establishes an objective function based on the parameter set, and optimizes the ablation parameters, ionization parameters, and mobility parameters based on the objective function and the optimization algorithm to obtain the target ablation parameters, target ionization parameters, and target mobility parameters.

[0063] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for measuring the three-dimensional distribution of a food and drug composition, characterized by, The method comprises the following steps: Step 1: placing a sample to be detected on a test bench, a first light emitter vertically aligned with the sample to be detected on the test bench emitting a first laser; Step 2: the sample to be detected generates gas molecules, the optical transceiver emits a second laser to the gas molecules to generate first spectral data, the first light emitter and the optical transceiver are closed according to the first spectral data, and the first time is recorded; Step 3: opening the first valve and the second valve, introducing a carrier gas, the gas molecules enter the ionization chamber, the ionization chamber generates an electrospray to ionize the gas molecules to generate sample ions, opening the third valve, the sample ions are introduced into the mobility chamber, and the second time is recorded; Step 4: the sample ions pass through the mobility chamber into the flight channel, the mass analyzer captures the sample ions to generate a first ion mobility spectrum, and the sampling data is intercepted; Step 5: repeating steps 1 to 4, obtaining a sampling data set, optimizing ablation parameters, ionization parameters and mobility parameters, obtaining a standard curve of a target component, preparing multiple composition slices of the sample to be detected, and placing the composition slices on the test bench according to the slice order; Step 6: obtaining two-dimensional data and thickness of the composition slice, and dividing a target grid; Step 7: moving the first light emitter and the optical transceiver according to the target grid, repeating steps 1 to 4, obtaining a second ion mobility spectrum, and calculating the content of the target component in the target grid; Step 8: if the target grid is completely traversed, it is judged whether the composition slice is completely traversed, if yes, step 9 is entered, otherwise the next composition slice is placed on the test bench, and step 6 is returned, otherwise the next target grid is entered, and step 7 is returned; Step 9: generating multiple three-dimensional slice models according to the two-dimensional data, thickness of the composition slice and the content of the target component in multiple target grids, and establishing a three-dimensional content model of the sample to be detected.

2. The method of claim 1, wherein the functional food composition is a food composition. In step 1, the test bench comprises a glass cover and a sample table, a vacuum environment is maintained in the glass cover, the sample to be detected is a thin slice, the sample to be detected is placed on the sample table, and the initial laser energy is set as a reference energy.

3. The method of claim 1, wherein the functional food composition is a food composition. In step 2, the optical transceiver comprises a second light emitter and a signal acquisition device, the signal acquisition device is installed with a band-pass filter, the signal acquisition device receives a fluorescent signal to generate first spectral data, the first spectral data is fluorescent spectral data, the first light emitter and the optical transceiver are closed when the fluorescent signal decreases to a baseline, and the first time is the closing time of the first light emitter and the optical transceiver.

4. The method of claim 1, wherein the functional food composition is a food composition. In step 3, the first valve controls the carrier gas to enter the test bench, the second valve controls the gas molecules to enter the ionization chamber, the third valve controls the sample ions to enter the mobility chamber, the carrier gas carries the gas molecules into the ionization chamber, the sample ions are introduced into the mobility chamber through an ion funnel, the carrier gas is helium, the ionization chamber is in a negative ion mode, the electrospray comprises methanol, water and formic acid, the mass fraction of formic acid is 0.1%, the volume ratio of methanol and water is 70:30, and the second time is the opening time of the third valve.

5. The method of claim 1, wherein the functional food composition is a food composition. In step 4, the first migration interval is calculated based on the first attribute of the target component and the buffer gas, the first flight interval is calculated based on the second attribute of the target component and the buffer gas, the sampling interval of the target component is calculated according to the first migration interval and the first flight interval, and the minimum sampling time and the maximum sampling time of the target component are obtained by combining the second time and the sampling interval, and the sampling data is intercepted from the first ion mobility spectrum according to the minimum sampling time and the maximum sampling time.

6. The method of claim 5, wherein the functional food composition is a food composition. In step 4, the mobility chamber contains a buffer gas, the buffer gas is helium, the flight channel is a high vacuum environment, the sampling data is a target ion peak, the target component is quercetin, the first attribute is an ion collision cross section, and the second attribute is a mass-to-charge ratio.

7. The method of claim 1, wherein the functional food composition is a food composition. In step 5, the sampling data set contains sampling data under multiple different parameters, the algorithm parameter set is obtained according to the sampling data set, the algorithm parameter set includes a symmetry factor and a peak width, a target function is established according to the algorithm parameter set, and the target ablation parameter, the target ionization parameter and the target mobility parameter are obtained by optimizing the ablation parameter, the ionization parameter and the mobility parameter according to the target function and the optimization algorithm, and the to-be-detected substance is a medicinal and edible composition.

8. The method of claim 6, wherein the functional food composition is a food composition. In step 7, the center of the light spot of the first light emitter is aligned with the center of the target grid, the first laser is perpendicular to the experimental table, the optical transceiver is parallel to the experimental table, the second laser is not in the same plane as the first laser, the minimum sampling time and the maximum sampling time are recalculated, the target ion peak of the second ion mobility spectrum is intercepted, and the content of the target component in the target grid is calculated according to the standard curve and the second ion mobility spectrum.

9. The method of claim 1, wherein the functional food composition is a food composition. In step 9, the target grid is aligned with the two-dimensional data of the composition slice, the content of the target component in the corresponding target grid is filled into the two-dimensional data, the three-dimensional slice model is generated by widening the two-dimensional data according to the thickness, and the three-dimensional content model of the to-be-detected substance is established by stacking multiple three-dimensional slice models in sequence.

10. A measuring device for measuring the method for measuring the three-dimensional distribution of the functional food composition according to claim 1, characterized by, Comprise: An experimental table, the experimental table comprises a glass cover and a sample table, the glass cover maintains a vacuum environment, and the sample table contains a to-be-detected sample and a composition slice; A first light emitter for emitting a first laser; An optical transceiver, the optical transceiver comprises a second light emitter and a signal acquisition device, the second light emitter is used for emitting a second laser, and the signal acquisition device is used for acquiring a fluorescence signal to generate first spectrum data; An ionization chamber for generating sample ions by ionizing gas molecules through electrospray; A mobility chamber for distinguishing target ions from other ions with similar mass-to-charge ratios; A flight channel for separating target ions from other ions with different mass-to-charge ratios by free flight in a high vacuum environment; A mass analyzer for capturing sample ions to generate a first ion mobility spectrum and a second ion mobility spectrum; A data processing device for calculating a minimum sampling time and a maximum sampling time, intercepting a target ion peak from the second ion mobility spectrum, obtaining the content of a target component in a target grid according to a standard curve, and establishing a three-dimensional content model of a to-be-detected substance. The data analysis device is used for analyzing the first time according to the first spectral data, obtaining an algorithm parameter set according to the sampling data set, establishing a target function according to the algorithm parameter set, and optimizing the ablation parameter, the ionization parameter and the mobility parameter according to the target function and an optimization algorithm. The three-dimensional positioning system is used for obtaining two-dimensional data and thickness of the composition slice, dividing a target grid, moving the first light emitter and the light transceiver according to the target grid, and replacing the composition slice to complete the measurement.

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