Preparation method of SUNPACLE light particle fertilizer synergist

Abstract

The invention relates to the technical field of fertilizer synergism, and discloses a SUNPACLE light particle fertilizer synergist preparation method, which comprises: in a reaction kettle, adding a nanometer photonic crystal material, a manganese-tyrosine compound and a phospholipid material into deionized water according to a certain ratio, and dissolving to obtain a light particle base liquid; and carrying out centrifugal separation and ultrasonic treatment on the light particle basic solution to obtain the SUNPACLE light particle fertilizer synergist. The utilization efficiency of the fertilizer can be improved, the photosynthesis of plants is promoted, and the environmental burden of the fertilizer is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizer synergists, and in particular to a method for preparing a SUNPACLE light particle fertilizer synergist. Background Art

[0002] In traditional agriculture, while fertilizer use can significantly increase crop yields in the short term, a significant portion of the nutrients often fail to be effectively absorbed by plants after application, resulting in a waste of fertilizer resources. For example, the utilization rate of nitrogen fertilizer is typically only 30%-50%, while that of phosphorus fertilizer is even lower. Furthermore, some fertilizers are easily lost to water, volatilized, or fixed in the soil, making them unusable by crops.

[0003] Furthermore, excessive use not only wastes fertilizer but can also cause environmental problems such as water and soil pollution. Especially after rainfall, fertilizer components (such as nitrogen and phosphorus) not absorbed by crops often enter water bodies through runoff, leading to eutrophication.

[0004] Therefore, how to improve fertilizer utilization efficiency and reduce the negative impact of fertilizers on the environment has become a difficult problem that needs to be solved urgently in modern agriculture. Summary of the Invention

[0005] In view of this, the present invention proposes a preparation method of SUNPACLE light particle fertilizer synergist, which aims to solve the problems of low fertilizer utilization efficiency and high environmental burden of fertilizer in current technology.

[0006] On the one hand, the present invention provides a method for preparing a SUNPACLE light particle fertilizer synergist, comprising the following steps:

[0007] In a reaction kettle, nanophotonic crystal materials, manganese-tyrosine complexes, and phospholipid materials are added to deionized water in proportion and dissolved to obtain a light particle base solution;

[0008] The light particle base liquid is centrifuged and ultrasonically treated to obtain the SUNPACLE light particle fertilizer synergist.

[0009] Furthermore, the nanophotonic crystal thin film material precursor is prepared by the following method:

[0010] 1 g of polystyrene microspheres with a diameter of 200 nm was added to 100 mL of deionized water, stirred for 30 min using a magnetic stirrer, and then sonicated for 10 min to obtain a PS dispersion.

[0011] The PS dispersion was dropped onto a clean glass slide and allowed to dry naturally at room temperature for 12 hours, allowing the PS microspheres to self-assemble into a photonic crystal array layer with structural color during the evaporation process.

[0012] The glass slide forming the PS photonic crystal was first immersed in a 0.1 mol / L ZnSO4 solution for 30 minutes, then transferred to a 0.1 mol / L TAA solution for another 30 minutes. Both reactions were carried out at 25°C. Zn2+ reacted with the slowly released S2- on the PS surface to form a dense and uniform ZnS shell.

[0013] The two-reaction process was repeated three times to obtain ZnS-PS composite microspheres with a shell thickness of 15-25 nm.

[0014] The ZnS-PS composite microspheres were scraped off the glass slide with a spatula, redispersed in 20 mL of deionized water, and subjected to ultrasound for 5 minutes to form a stable suspension to obtain the nanophotonic crystal material.

[0015] Furthermore, the magnetic stirring speed in the nano-photonic crystal thin film material precursor is 300-500 rpm.

[0016] Furthermore, the configuration process of the manganese-tyrosine complex is:

[0017] Weigh 0.1 g of MnSO4·H2O and dissolve it in 100 mL of deionized water. Add 0.3 g of L-tyrosine and continue stirring for 10 minutes.

[0018] NaOH was added to ionize the phenolic hydroxyl group, and the reaction was carried out at room temperature for 30 minutes to form a light brown or light yellow transparent liquid, which was filtered to obtain a manganese-tyrosine complex.

[0019] Furthermore, after adding NaOH, the pH was controlled to be maintained at 6.5-7.0.

[0020] Furthermore, during the preparation of the manganese-tyrosine complex, the filtration is to remove free particles or uncoordinated substances through a 0.22 μm filter membrane.

[0021] Furthermore, the phospholipid material is prepared by the following method:

[0022] Weigh 1 g of lecithin and add it to a 100 mL beaker. Add 0.5 g of glycerol and 98.5 g of deionized water. Stir at room temperature for 5 minutes to obtain a mixed solution.

[0023] Use a probe sonicator, insert the probe into the mixed solution, set the ultrasonic power to 200W, and the ultrasonic time to 5 minutes, with a 10-second pause every 30 seconds. During the ultrasonication, place the beaker in a constant temperature water bath at 25°C.

[0024] After the ultrasound, a lecithin solution with a particle size of 80-150 nm is formed;

[0025] The emulsion after ultrasonication was transferred to a magnetic stirrer, the temperature was controlled at 25-35°C, and stirred for 60 minutes. After the aging was completed, a phospholipid material was obtained.

[0026] Furthermore, the volume ratio of the nano-photonic crystal material, the manganese-tyrosine complex, the phospholipid material and the deionized water is 10-12:1-3:2-4:3-6.

[0027] Furthermore, the centrifugal separation is performed at 10,000-15,000 rpm for 10-20 min at a temperature of 4°C;

[0028] The ultrasonic treatment is performed at 20-40 kHz for 5-15 minutes.

[0029] On the other hand, the present invention also protects a fertilizer synergist obtained by the above preparation method.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] By adding nanophotonic crystal materials, particularly photonic crystal thin films, to fertilizer synergists, they can selectively enhance light intensity within the spectral band (450-680nm). These photonic crystal materials can significantly increase the light absorption efficiency of plant leaves, especially in the visible and ultraviolet light ranges, thereby improving the efficiency of plant photosynthesis. By optimizing the utilization of light energy, photonic crystal materials increase the absorption capacity of chlorophyll, thereby accelerating the plant's photosynthesis process and providing more energy for plant growth.

[0032] Nanophotonic crystals effectively transmit external light energy to plant chloroplasts through their optical effects, ensuring that plants can carry out efficient photosynthesis under more light conditions. This photosynthesis enhancement effect is particularly important in increasing crop growth and yield.

[0033] Manganese-tyrosine complexes play a crucial role in plant photosystem II (PSI I) by enhancing its stability and promoting electron transport. Manganese ions, a crucial component of PSI I, accelerate electron transport. The addition of tyrosine helps stabilize the reaction center, further enhancing photosynthetic efficiency. Under high temperatures or intense light, manganese-tyrosine complexes can effectively reduce damage to PSI II, enabling plants to continue photosynthesis under these harsh conditions.

[0034] Manganese-tyrosine complex can effectively improve the anti-photoinhibition ability of photosystem II, delay the photoinhibition phenomenon of plant leaves under strong light irradiation, prolong the photosynthetic reaction time of plants, and improve the utilization rate of light energy.

[0035] Phospholipid materials are used to mimic plant cell membrane structure and optimize membrane protein activity during photosynthesis. By increasing the density of thylakoid membrane ATP synthase and the activity of C4 plant PEP carboxylase, phospholipid materials promote ATP production and CO2 fixation efficiency, thereby significantly improving the overall efficiency of photosynthesis.

[0036] By increasing the density of ATP synthase and the activity of PEP carboxylase, phospholipid materials optimize the efficiency of energy conversion during photosynthesis and enhance the plant's adaptability to the environment. Furthermore, by enhancing membrane stability, phospholipid materials enable each step of photosynthesis to proceed more efficiently, avoiding energy loss caused by membrane instability.

[0037] By using this light particle fertilizer synergist, the photosynthesis efficiency of plants is significantly improved, thereby improving the absorption and utilization of nutrients in fertilizers. The synergist can promote plants to absorb nitrogen, phosphorus, potassium and other nutrients in the soil more efficiently, thereby reducing fertilizer waste.

[0038] Due to the improved efficiency of photosynthesis, plants can better utilize nutrients in fertilizers during their growth, reducing the amount of fertilizer lost to water, volatilization, or unabsorbed fertilizer components that remain fixed in the soil. This not only helps reduce fertilizer waste, but also effectively reduces fertilizer pollution to soil and water sources, alleviates eutrophication of water bodies, and protects the environment.

[0039] Because the manganese-tyrosine complex enhances the stability of photosystem II and the phospholipid material optimizes the membrane structure, the light particle fertilizer synergist can help plants maintain good growth in adverse environments such as high temperature, strong light, drought, or salinity, thereby enhancing their stress resistance. Crops can effectively maintain normal photosynthesis in more adverse environments, reducing growth stagnation or yield reduction caused by adverse conditions. DETAILED DESCRIPTION

[0040] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0041] Example 1

[0042] Step S1: Preparation of light particle base solution:

[0043] Nanophotonic crystal material: Take 10 mL (volume ratio is 10), add 30 mL of deionized water, and stir using a magnetic stirrer for 30 minutes.

[0044] Manganese-tyrosine complex: Add 1 mL of manganese-tyrosine solution (containing 0.1 g MnSO4·H2O and 0.3 g L-tyrosine) and continue stirring for 10 minutes.

[0045] Phospholipid materials: Add 2 mL of lecithin solution (lecithin: glycerol: water = 1:0.5:98.5) and stir for 5 minutes.

[0046] Step S2: Ultrasonic treatment and centrifugal separation:

[0047] Ultrasonic treatment: Set the ultrasonic power to 200 W and the frequency to 30 kHz for 10 minutes, with a 10-second pause every 30 seconds.

[0048] Centrifugation: centrifuge at 12000 rpm for 15 minutes at 4°C.

[0049] Step S3: Final filtration:

[0050] Filter using a 0.22 μm filter membrane to obtain 20 mL of the final enhancer solution.

[0051] Example 2

[0052] Step S1: Preparation of light particle base solution:

[0053] Nanophotonic crystal material: Take 12 mL (volume ratio is 12), add 30 mL of deionized water, and stir using a magnetic stirrer for 30 minutes.

[0054] Manganese-tyrosine complex: Add 1 mL of manganese-tyrosine solution (containing 0.1 g MnSO4·H2O and 0.3 g L-tyrosine) and continue stirring for 10 minutes.

[0055] Phospholipid materials: Add 2.5 mL of lecithin solution (lecithin: glycerol: water = 1:0.5:98.5) and stir for 5 minutes.

[0056] Step S2: Ultrasonic treatment and centrifugal separation:

[0057] Ultrasonic treatment: Set the ultrasonic power to 250 W and the frequency to 40 kHz for 15 minutes, with a 10-second pause every 30 seconds.

[0058] Centrifugation: centrifuge at 10,000 rpm for 20 minutes at 4°C.

[0059] Step S3: Final filtration:

[0060] Filter using a 0.22 μm filter membrane to obtain 18 mL of the final enhancer solution.

[0061] Example 3

[0062] Step S1: Preparation of light particle base solution:

[0063] Nanophotonic crystal material: Take 8 mL (volume ratio is 10), add 32 mL of deionized water, and stir using a magnetic stirrer for 30 minutes.

[0064] Manganese-tyrosine complex: Add 1 mL of manganese-tyrosine solution (containing 0.1 g MnSO4·H2O and 0.3 g L-tyrosine) and continue stirring for 10 minutes.

[0065] Phospholipid materials: Add 2 mL of lecithin solution (lecithin: glycerol: water = 1:0.5:98.5) and stir for 5 minutes.

[0066] Step S2: Ultrasonic treatment and centrifugal separation:

[0067] Ultrasonic treatment: Set the ultrasonic power to 200 W and the frequency to 30 kHz for 5 minutes, with a 10-second pause every 30 seconds.

[0068] Centrifugation: centrifuge at 15000 rpm for 10 minutes at 4°C.

[0069] Step S3: Final filtration

[0070] Filter using a 0.22 μm filter membrane to obtain 22 mL of the final enhancer solution.

[0071] In order to verify the effectiveness of the fertilizer synergist proposed in this invention, a series of experiments were designed to investigate plant growth, photosynthesis efficiency, stress resistance, and nutrient absorption. The experimental design and experimental data are as follows:

[0072] Experimental Materials:

[0073] Crop options: corn, wheat, tomatoes

[0074] Fertilizer types: light particle fertilizer, NPK fertilizer (nitrogen, phosphorus, and potassium mixed fertilizer, hereinafter referred to as NPK fertilizer); the light particle fertilizer is prepared by adding the fertilizer synergist of Example 1 to the NPK fertilizer and mixing them, with the addition amount being 1 g of fertilizer synergist per 10 g of NPK fertilizer.

[0075] Experimental design: Using a controlled experimental method, two groups were set up for each crop: Experimental group: light particle fertilizer was applied. Control group: traditional nitrogen, phosphorus, and potassium fertilizers were applied.

[0076] Experimental steps: Soil preparation: Select the same type of soil from the same location and conduct basic fertility tests (pH, moisture content, nitrogen, phosphorus, and potassium content). Plants in each experimental and control group are grown under the same conditions, ensuring consistent growth conditions such as light and temperature.

[0077] Fertilization: Light particle fertilizer and NPK fertilizer were applied to the experimental group and control group respectively according to the above method. Fertilizer application methods included foliar spray (light particle fertilizer) and root irrigation (NPK fertilizer).

[0078] Growth monitoring: Regularly monitor plant growth every week, including plant height, leaf area, root development, etc.

[0079] To determine the photosynthetic efficiency of crops, a photosynthetic meter was used to measure the photosynthetic rate and transpiration rate of plants.

[0080] Stress treatment: A portion of the plants were subjected to drought treatment (stop irrigation for one week) and salinity treatment (add appropriate amount of salt to the irrigation water) to test the effect of fertilizer on crop stress resistance.

[0081] The evaluation is carried out by measuring plant stress resistance-related indicators (such as leaf water content, salt tolerance, etc.).

[0082] The experimental data are shown in Tables 1 to 4.

[0083] Table 1 Plant growth effect

[0084]

[0085] Table 2 Photosynthesis efficiency

[0086]

[0087]

[0088] Table 3 Stress resistance test

[0089]

[0090] Table 4 Nutrient absorption efficiency

[0091]

[0092] Experimental conclusion:

[0093] Growth effect: All crops showed higher growth rate, leaf area and root development after application of light particle fertilizer, which shows that light particle fertilizer has higher fertilizer utilization efficiency and can promote healthy growth of plants.

[0094] Photosynthesis: The photosynthesis efficiency of crops treated with light particle fertilizer was significantly improved, indicating that light particle fertilizer can enhance the plant's utilization of light energy, thereby providing more growth support.

[0095] Stress resistance: Under drought and saline-alkali conditions, crops treated with light particle fertilizers showed higher stress resistance and maintained better leaf water content, indicating that light particle fertilizers help enhance the adaptability of crops in harsh environments.

[0096] Nutrient absorption: Light particle fertilizer significantly improves the crop's absorption efficiency of nutrients such as nitrogen, phosphorus, and potassium, and reduces fertilizer waste, indicating that this fertilizer has significant advantages in fertilizer resource utilization and environmental protection.

[0097] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered by the scope of protection of the claims of the present invention.

Claims

1. A method for preparing SUNPACLE light particle fertilizer synergist, characterized in that: The following steps are involved: In a reaction kettle, nanophotonic crystal materials, manganese-tyrosine complexes, and phospholipid materials are added to deionized water in proportion and dissolved to obtain a light particle base solution; The light particle base liquid is centrifuged and ultrasonically treated to obtain the SUNPACLE light particle fertilizer synergist.

2. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 1, characterized in that: The nanophotonic crystal thin film material precursor is prepared by the following method: 1 g of polystyrene microspheres with a diameter of 200 nm was added to 100 mL of deionized water, stirred for 30 min using a magnetic stirrer, and then sonicated for 10 min to obtain a PS dispersion. The PS dispersion was dropped onto a clean glass slide and allowed to dry naturally at room temperature for 12 hours, allowing the PS microspheres to self-assemble into a photonic crystal array layer with structural color during the evaporation process. The glass piece forming the PS photonic crystal was immersed in a 0.1 mol / L ZnSO4 solution for 30 minutes, and then transferred to a 0.1 mol / L TAA solution for 30 minutes. The two reactions were carried out at 25 °C. 2+ With slow release of S 2- Reacts on the PS surface to form a dense and uniform ZnS shell; The two-reaction process was repeated three times to obtain ZnS-PS composite microspheres with a shell thickness of 15-25 nm. The ZnS-PS composite microspheres were scraped off the glass slide with a spatula, redispersed in 20 mL of deionized water, and subjected to ultrasound for 5 minutes to form a stable suspension to obtain the nanophotonic crystal material.

3. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 2, characterized in that: The magnetic stirring speed in the nano-photonic crystal thin film material precursor is 300-500 rpm.

4. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 1, characterized in that: The configuration process of the manganese-tyrosine complex is: Weigh 0.1 g of MnSO4·H2O and dissolve it in 100 mL of deionized water. Add 0.3 g of L-tyrosine and continue stirring for 10 minutes. NaOH was added to ionize the phenolic hydroxyl group, and the reaction was carried out at room temperature for 30 minutes to form a light brown or light yellow transparent liquid, which was filtered to obtain a manganese-tyrosine complex.

5. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 4, characterized in that: After adding NaOH, the pH was controlled to be maintained at 6.5-7.

0.

6. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 4, characterized in that: During the preparation of the manganese-tyrosine complex, the filtration is performed by using a 0.22 μm filter membrane to remove free particles or uncoordinated substances.

7. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 1, characterized in that: The phospholipid material is prepared by the following method: Weigh 1 g of lecithin and add it to a 100 mL beaker. Add 0.5 g of glycerol and 98.5 g of deionized water. Stir at room temperature for 5 minutes to obtain a mixed solution. Use a probe sonicator, insert the probe into the mixed solution, set the ultrasonic power to 200W, and the ultrasonic time to 5 minutes, with a 10-second pause every 30 seconds. During the ultrasonication, place the beaker in a constant temperature water bath at 25°C. After the ultrasound, a lecithin solution with a particle size of 80-150 nm is formed; The emulsion after ultrasonication was transferred to a magnetic stirrer, the temperature was controlled at 25-35°C, and stirred for 60 minutes. After the aging was completed, a phospholipid material was obtained.

8. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 1, characterized in that: The volume ratio of the nano-photonic crystal material, the manganese-tyrosine complex, the phospholipid material and the deionized water is 10-12:1-3:2-4:3-6.

9. The method for preparing a SUNPACLE light particle fertilizer synergist according to claim 1, characterized in that The centrifugal separation is performed at 10,000-15,000 rpm for 10-20 min at a temperature of 4° C. The ultrasonic treatment is performed at 20-40 kHz for 5-15 minutes.

10. A fertilizer synergist obtained according to the preparation method according to any one of claims 1 to 9.

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