Ultra-micro spectrophotometer with variable range and use method of ultra-micro spectrophotometer

Abstract

The invention relates to the technical field of spectrophotometers, in particular to an ultramicro spectrophotometer with a variable range, which comprises a base assembly, an ultramicro spectrophotometer, an ultramicro spectrophotometer and an ultramicro spectrophotometer, the base assembly comprises a lower base and an upper base cover, and the lower base and the upper base cover are hinged through a rotating shaft; the adjusting mechanism comprises an optical fiber shaft and a linear motor, and the optical fiber shaft is installed on the lower base through a limiting bearing; the linear motor is connected with the optical fiber shaft through a transmission structure and drives the optical fiber shaft to axially move; the xenon lamp optical fiber is fixedly arranged on the lower base; the spectrum fiber is fixed at one end of the fiber shaft; the flip optical fiber is arranged on the upper base cover and comprises two optical fiber heads, a center hole of one optical fiber head is coaxially aligned with a center hole of the xenon lamp optical fiber, and a center hole of the other optical fiber head is coaxially aligned with a center hole of the spectrum optical fiber. Through cooperation of the linear motor and the optical fiber shaft, the distance from the spectrum optical fiber to the flip optical fiber is adjustable, and the measuring range of the ultramicro spectrophotometer can be adjusted according to requirements.

Description

Technical Field

[0001] The present invention relates to the technical field of spectrophotometers, in particular to an ultra-micro spectrophotometer with a variable range and a use method thereof. Background Art

[0002] An ultra-micro-volume spectrophotometer is an instrument that integrates nucleic acid detection, protein detection, cell solution detection, and full-wavelength spectral detection. It needs to quickly and accurately detect nucleic acids, proteins, and cell solutions, so its precise structure is crucial to making this compact instrument popular and recognized in fields ranging from biological laboratories to research institutes.

[0003] The accuracy of ultra-micro-spectrophotometer test results depends on the alignment of the test hole base. However, the rotation of the upper and lower bases of existing ultra-micro-spectrophotometers affects the accuracy of fiber alignment, and the fiber measurement travel is limited, which cannot meet the requirements of ensuring accurate fiber alignment within a variable travel. Summary of the Invention

[0004] The object of the present invention is to provide a variable range ultra-micro spectrophotometer and a method of use, which solves the problems raised by the above background technology.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: a variable-range ultra-micro spectrophotometer, comprising: a base assembly, the base assembly comprising a lower base and an upper base cover, the lower base and the upper base cover being hingedly connected via a rotating shaft; An adjustment mechanism includes an optical fiber shaft and a linear motor. The optical fiber shaft is mounted on the lower base via a limit bearing. The linear motor is connected to the optical fiber shaft via a transmission structure to drive the optical fiber shaft to move axially. A xenon lamp optical fiber, wherein the xenon lamp optical fiber is fixedly arranged on the lower base; A spectrum optical fiber, wherein the spectrum optical fiber is fixed to one end of the optical fiber shaft; The flip-up optical fiber is arranged on the upper base cover and includes two optical fiber heads, wherein the center hole of one optical fiber head is coaxially aligned with the center hole of the xenon lamp optical fiber, and the center hole of the other optical fiber head is coaxially aligned with the center hole of the spectrum optical fiber.

[0006] Furthermore, as a preferred embodiment of the present invention, a sensor sheet is provided on the surface of the optical fiber shaft, and the sensor sheet is connected to a position sensor, and the position sensor is used to monitor the position of the optical fiber shaft.

[0007] Furthermore, as a preferred embodiment of the present invention, a fiber axis placement hole is provided on the lower base, the spectrum optical fiber passes through the fiber axis placement hole, and the spectrum optical fiber is gap-fitted with the fiber axis placement hole.

[0008] Furthermore, as a preferred embodiment of the present invention, the upper base cover is provided with a dust cover covering the rotating shaft, and a dust pad is provided on the upper surface of the optical fiber shaft placement hole.

[0009] Furthermore, as a preferred embodiment of the present invention, the rotating shaft passes through the lower base and the upper base cover, and is mounted on the lower base through a bearing and a shaft ring, so that the upper base cover maintains coaxiality when rotating around the rotating shaft.

[0010] Furthermore, as a preferred embodiment of the present invention, a limit bearing is externally mounted on the optical fiber shaft, and the limit bearing is used to limit the radial movement range of the optical fiber shaft.

[0011] Furthermore, as a preferred embodiment of the present invention, the linear motor is fixed on a motor base, and the top of the motor base is fixed to the lower surface of the lower base by screws.

[0012] In the present invention, a method for using a variable-range ultramicro spectrophotometer comprises the following steps: S1. Coaxial alignment: Pass the centering axis through the upper base cover, the rotating shaft and the lower base, and adjust the center hole of the flip cover optical fiber to align coaxially with the center hole of the xenon lamp optical fiber; S2. Parallel positioning: Place the parallel tool on the surface of the lower base so that the upper base cover and the lower base are equidistant, and insert the positioning pins to fix the distance; S3. Base locking: Use locking screws to fasten the shaft to the lower base and upper base cover; S4. Fiber fine-tuning: Adjust the spectrum fiber by adjusting the horizontal displacement of the left and right adjustment tool, and adjust the longitudinal displacement of the front and back adjustment tool to achieve alignment with the center of the flip-cover fiber. The left and right adjustment tool and the front and back adjustment tool are installed on the periphery of the fiber axis; S5, variable stroke detection: Start the linear motor to drive the optical fiber axis to move, and complete the sample detection under the constraint of the limit bearing.

[0013] Beneficial effects: The technical solution of this application has the following technical effects: 1. The distance between the spectral optical fiber and the flip-cover optical fiber can be adjusted by the cooperation of the linear motor and the optical fiber shaft, so that the range of the ultra-micro spectrophotometer can be adjusted according to needs; 2. By first using the center holes of the xenon lamp fiber and the flip-cover fiber as a reference, adjust the center alignment of the flip-cover fiber and the spectrum fiber; fix one end and adjust the other end, which can reduce the difficulty of component processing of the equipment and thus reduce costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1It is a schematic diagram of the structure of the present invention; Figure 2 for Figure 1 Structural diagram of the cross section along the middle A-A'; Figure 3 for Figure 1 Structural diagram of the cross section along the middle B-B'; Figure 4 A schematic diagram of a device for the calibration process in the method of use of the present invention; Figure 5 for Figure 4 Structural diagram of the cross section along the middle C-C'; Figure 6 for Figure 4 Cross-sectional structural diagram along the D-D' line.

[0015] In the figure, the meanings of the reference numerals are as follows: 1, lower base; 2, upper base cover; 3, rotating shaft; 4, bearing; 5, shaft collar; 6, dust cover; 7, flip-up optical fiber; 8, xenon lamp optical fiber; 9, spectrum optical fiber; 10, limit bearing; 11, optical fiber shaft; 12, linear motor; 13, dustproof pad; 14, motor support frame; 15. Position sensor; 16. Sensor plate; 17. Bracket; 18. Centering axis; 19. Parallel tooling; 20. Left and right adjustment tooling; 21. Fore and aft adjustment tooling; 22. Positioning pin. DETAILED DESCRIPTION

[0016] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0017] As attached Figure 1 To the attached Figure 3 As shown: This embodiment provides a variable range ultra-micro spectrophotometer, a base assembly, the base assembly includes a lower base 1 and an upper base cover 2, the lower base 1 and the upper base cover 2 are hinged by a rotating shaft 3; An adjustment mechanism includes an optical fiber shaft 11 and a linear motor 12. The optical fiber shaft 11 is mounted on the lower base 1 via a limit bearing 10. The linear motor 12 is connected to the optical fiber shaft 11 via a transmission structure (such as a lead screw, a gear rack, etc.) to drive the optical fiber shaft 11 to move axially. A xenon lamp optical fiber 8, wherein the xenon lamp optical fiber 8 is fixedly arranged on the lower base 1; A spectrum optical fiber 9, wherein the spectrum optical fiber 9 is fixed to one end of an optical fiber shaft 11; The flip-up optical fiber 7 is disposed on the upper base cover 2 and includes two optical fiber tips. The center hole of one optical fiber tip is coaxially aligned with the center hole of the xenon lamp optical fiber 8, and the center hole of the other optical fiber tip is coaxially aligned with the center hole of the spectrum optical fiber 9. The two optical fiber tips of the flip-up optical fiber are connected by an optical fiber.

[0018] The surface of the optical fiber shaft 11 is provided with a sensor sheet 16, which is connected to a position sensor 15. The position sensor 15 is used to monitor the position of the optical fiber shaft 11. The lower base 1 is provided with an optical fiber shaft mounting hole, through which the spectral optical fiber 9 passes, with a clearance fit between the optical fiber shaft mounting hole and the spectral optical fiber 9. The upper base cover 2 is provided with a dust cover 6 covering the rotating shaft 3, and a dustproof pad 13 is provided on the upper surface of the optical fiber shaft mounting hole. The linear motor 12 is fixed to the motor base 14, the top of which is fixed to the lower surface of the lower base 1 by screws.

[0019] Furthermore, the rotating shaft 3 passes through the lower base 1 and the upper base cover 2 and is mounted to the lower base 1 via a bearing 4 and a collar 5, ensuring that the upper base cover 2 maintains coaxiality when rotating about the rotating shaft 3. The optical fiber shaft 11 is externally coupled with a limit bearing 10. The use of the limit bearing also effectively limits the radial direction of the optical fiber shaft, preventing the linear motor from shaking when driving the optical fiber shaft in axial motion. This could cause significant radial deviation of the optical fiber on the optical fiber shaft, affecting the alignment of the optical fiber and the flip cover optical fiber.

[0020] Reference Figure 4-6 The following describes a method for using a variable-range ultra-micro spectrophotometer. The method comprises the following steps: S1. Coaxial alignment: Insert the centering shaft 18 through the upper base cover 2, the rotating shaft 3 and the lower base 1, and adjust the center hole of the flip cover optical fiber 7 and the xenon lamp optical fiber 8 to be coaxially aligned; S2. Parallel positioning: Place the parallel tool 19 on the surface of the lower base 1 so that the upper base cover 2 and the lower base 1 are equidistant, and insert the positioning pins 22 to fix the distance; S3, base locking: Use locking screws to fasten the shaft 3 to the lower base 1 and the upper base cover 2; S4. Fiber fine-tuning: Adjust the spectrum fiber 9 laterally by the left-right adjustment tool 20, and adjust the spectrum fiber 9 longitudinally by the front-back adjustment tool 21, so as to achieve alignment with the center of the flip-cover fiber 7. The left-right adjustment tool 20 and the front-back adjustment tool 21 are placed on the periphery of the fiber axis 11; S5, variable stroke detection: start the linear motor 12 to drive the optical fiber shaft 11 to move, and complete the sample detection under the constraint of the limit bearing 10.

[0021] Among them, the centering axis is a cylindrical shaft rod, whose diameter is slightly smaller than the axial hole on the rotating shaft 3. The centering axis passes through the fiber optic head placement hole of the flip-up optical fiber on the upper base cover 2, the axial hole of the rotating shaft 3 and the xenon lamp optical fiber placement hole on the lower base 1 in sequence to realize the alignment of the flip-up optical fiber head placement hole and the xenon lamp optical fiber placement hole. After alignment, the centering axis is taken out, and the flip-up optical fiber and the xenon lamp optical fiber are installed in the flip-up optical fiber head placement hole and the flip-up optical fiber head placement hole respectively.

[0022] The shape of the parallel tooling 19 is identical to that of the upper base, and the thickness of the parallel tooling 16 is the required spacing from the upper base to the lower base. Inserting the parallel tooling 16 between the upper base and the lower base ensures that the spacing between the upper base and the lower base is consistent.

[0023] The shape of the front and rear adjustment tooling 21 is the same as that of the motor support frame, and the left and right adjustment tooling 20 is sheet-shaped. During calibration, the front and rear adjustment tooling is inserted into the interior of the motor support frame and sleeved on the optical fiber shaft. Then the left and right adjustment tooling 20 is installed on the front and rear adjustment tooling 21. The position of the optical fiber shaft is fine-tuned by adjusting the screws inserted on the front and rear adjustment tooling and the left and right adjustment tooling.

[0024] Before performing variable range detection, start the xenon lamp fiber and check the transmission of the light beam to determine whether the flip-cover fiber is aligned with the xenon lamp fiber and whether the flip-cover fiber is aligned with the spectrum fiber.

[0025] The above is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modifications to equivalent embodiments of the technical content disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A variable range ultra-micro spectrophotometer, characterized in that: include: A base assembly, the base assembly comprising a lower base (1) and an upper base cover (2), the lower base (1) and the upper base cover (2) being hinged via a rotating shaft (3); An adjustment mechanism, the adjustment mechanism comprising an optical fiber shaft (11) and a linear motor (12), wherein the optical fiber shaft (11) is mounted on the lower base (1) via a limit bearing (10); the linear motor (12) is connected to the optical fiber shaft (11) via a transmission structure to drive the optical fiber shaft (11) to move axially; A xenon lamp optical fiber (8), wherein the xenon lamp optical fiber (8) is fixedly arranged on the lower base (1); A spectrum optical fiber (9), wherein the spectrum optical fiber (9) is fixed to one end of the optical fiber shaft (11); A flip-up optical fiber (7) is provided on the upper base cover (2), and comprises two optical fiber heads, wherein the center hole of one optical fiber head is coaxially aligned with the center hole of the xenon lamp optical fiber (8), and the center hole of the other optical fiber head is coaxially aligned with the center hole of the spectrum optical fiber (9).

2. The variable range ultra-micro spectrophotometer according to claim 1, characterized in that: A sensing sheet (16) is provided on the surface of the optical fiber shaft (11), and the sensing sheet (16) is connected to a position sensor (15). The position sensor (15) is used to monitor the position of the optical fiber shaft (11).

3. The variable range ultra-micro spectrophotometer according to claim 1, characterized in that: The lower base (1) is provided with an optical fiber shaft placement hole, the spectrum optical fiber (9) passes through the optical fiber shaft placement hole, and the spectrum optical fiber (9) is clearance-matched with the optical fiber shaft placement hole.

4. The variable-range ultra-micro spectrophotometer according to claim 3, characterized in that: The upper base cover (2) is provided with a dust cover (6) covering the rotating shaft (3), and a dust pad (13) is provided on the upper surface of the optical fiber shaft placement hole.

5. The variable range ultra-micro spectrophotometer according to claim 1, characterized in that: The rotating shaft (3) passes through the lower base (1) and the upper base cover (2), and is mounted on the lower base (1) via a bearing (4) and a shaft ring (5), so that the upper base cover (2) maintains coaxiality when rotating around the rotating shaft (3).

6. The variable-range ultra-micro spectrophotometer according to claim 1, characterized in that: The optical fiber shaft (11) is outer-mounted with a limiting bearing (10), and the limiting bearing (10) is used to limit the radial movement range of the optical fiber shaft (11).

7. The variable-range ultra-micro spectrophotometer according to claim 1, characterized in that: The linear motor (12) is fixed on a motor seat (14), and the top of the motor seat (14) is fixed to the lower surface of the lower base (1) by screws.

8. A method for using a variable-range ultramicro spectrophotometer, wherein the ultramicro spectrophotometer is the ultramicro spectrophotometer according to any one of claims 1 to 7, characterized in that: The steps include: S1. Coaxial alignment: insert the centering shaft (18) through the upper base cover (2), the rotating shaft (3) and the lower base (1), and adjust the center hole of the flip cover optical fiber (7) and the xenon lamp optical fiber (8) to be coaxially aligned; S2. Parallel positioning: Place the parallel tool (19) on the surface of the lower base (1) so that the upper base cover (2) and the lower base (1) are equidistant, and insert the positioning pin (22) to fix the spacing; S3, base locking: use locking screws to fasten the shaft (3) to the lower base (1) and the upper base cover (2); S4, optical fiber fine-tuning: adjusting the spectral optical fiber (9) by lateral displacement of the left-right adjustment tool (20), and adjusting the spectral optical fiber (9) by longitudinal displacement of the front-back adjustment tool (21), so as to achieve alignment with the center of the flip optical fiber (7), wherein the left-right adjustment tool (20) and the front-back adjustment tool (21) are placed on the periphery of the optical fiber axis (11); S5, variable stroke detection: start the linear motor (12) to drive the optical fiber shaft (11) to move, and complete the sample detection under the constraint of the limit bearing (10).

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