Graded ultrafiltration purification system for cartilage stem cell paracrine substances and application of graded ultrafiltration purification system

Abstract

The invention relates to a grading ultrafiltration purification system for cartilage stem cell paracrine substances and application thereof, and belongs to the technical field of microbiological treatment.The grading ultrafiltration purification system comprises a mounting ring, a separation ring is fixedly mounted in the mounting ring, an ultrafiltration cylinder is fixedly mounted at the upper end of the interior of the mounting ring, an ultrafiltration assembly is mounted in the ultrafiltration cylinder, and a cylinder cover is mounted at the upper end of the ultrafiltration cylinder; communicating holes are formed in the lower end of the interior of the ultrafiltration cylinder in a circumferential array mode, a bearing cylinder is installed at the lower end of the interior of the installation ring in a threaded mode, a bearing assembly is installed in the bearing cylinder, a temperature adjusting assembly is installed at the lower end of the exterior of the bearing cylinder, and supporting rods are fixedly installed at the lower end of the temperature adjusting assembly in a circumferential array mode. The ultra-filtration device is simple in structure and convenient for a user to clean, the ultra-filtered substance is temporarily stored in the receiving assembly, the storage temperature can be adjusted through the temperature adjusting assembly in the temporary storage process, so that the storage effect is improved, and the receiving assembly can be lifted during taking, so that the user can conveniently take out the substance.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial processing, in particular to a graded ultrafiltration purification system for cartilage stem cell paracrine substances and applications thereof. Background Art

[0002] The paracrine substances of cartilage stem cells play a key role in cartilage repair, regeneration and maintenance of cartilage homeostasis. These paracrine substances mainly include growth factors, cytokines, exosomes and other bioactive molecules. The paracrine substances of cartilage stem cells regulate the metabolism, proliferation, differentiation and synthesis and degradation of extracellular matrix of cartilage cells through complex signaling networks. The paracrine substances of cartilage stem cells will be ultrafiltered and purified when they are taken. The paracrine substances of cartilage stem cells may contain cell debris, culture medium and other substances. Impurities such as base components and protein aggregates not only affect the accurate analysis of paracrine substances, but may also interfere with their effectiveness in subsequent experiments or treatments. At the same time, the ultrafiltration and purification processes are usually carried out under mild conditions, avoiding factors such as high temperature and extreme pH values ​​that may destroy the biological activity of paracrine substances. Therefore, it is necessary to ultrafilter and purify the paracrine substances of cartilage stem cells. However, the existing ultrafiltration purification device is not convenient for replacing the molecular filter plate during use. At the same time, the ultrafiltered substance cannot be stored and is easily inactivated due to changes in the external environmental temperature. In view of this, the present invention is specially proposed. Summary of the Invention

[0003] In order to overcome the technical defects of the existing technology, the present invention provides a graded ultrafiltration purification system for cartilage stem cell paracrine substances and its application, which is convenient to use and has good substance preservation effects. The ultrafiltration component can be installed in the ultrafiltration cartridge through the cartridge cover, and the ultrafiltration component can perform ultrafiltration. At the same time, the ultrafiltration component can be disassembled for user convenience. The ultrafiltered substance falls through the connecting hole onto the receiving component for temporary storage. During the temporary storage process, the storage temperature can be adjusted by the temperature control component, thereby improving the storage effect.

[0004] The technical solution adopted by the present invention is: it includes a mounting ring, a separator ring is fixedly installed inside the mounting ring, an ultrafiltration cartridge is fixedly installed on the upper end of the inner part of the mounting ring, the ultrafiltration cartridge is located at the upper end of the separator ring, and an ultrafiltration component is installed inside the ultrafiltration cartridge, a cartridge cover is installed on the upper end of the ultrafiltration component, a connecting hole is provided in a circumferential array at the lower end of the inner part of the ultrafiltration cartridge, a receiving cartridge is threadedly installed on the lower end of the inner part of the mounting ring, a lifting receiving component is installed inside the receiving cartridge, and an adjusting cartridge is installed on the outer lower end of the receiving cartridge. Temperature component, the lower end circumferential array of the temperature control component is fixedly installed with support rods. When in use, first place this structure at the place of use, then put the cartilage stem cell paracrine substance into the ultrafiltration component, and then fix and seal it through the cylinder cover. The ultrafiltration component can ultrafilter, and the ultrafiltered substance will enter the side wall of the ultrafiltration cylinder, and under the action of gravity, it will fall through the connecting hole to the receiving culture dish in the receiving component for temporary storage. During the storage process, the temperature control component can adjust the temperature, thereby improving the storage effect and reducing the occurrence of substance inactivation.

[0005] Preferably, in order to be able to install this structure at an external use location, sealing gaskets are fixedly installed on the upper and lower ends of the separating ring, the outer lower end of the ultrafiltration cylinder and the upper end of the receiving cylinder are in close contact with the sealing gaskets, and the outer array of the mounting ring is fixedly installed with a mounting rod, the shape of the mounting rod is L-shaped, and the other end of the mounting rod is provided with a circular array of mounting holes.

[0006] Preferably, in order to enable the rotating disk to rotate in the ultrafiltration cartridge, a rotating hole in the shape of a cross is opened in the middle position of the lower end of the ultrafiltration cartridge, the ultrafiltration component includes a control motor, and the output end of the control motor is fixedly installed with a rotating shaft, the rotating shaft is rotatably clamped in the rotating hole, and the upper end of the rotating shaft is fixedly installed with a rotating disk, the upper end of the rotating disk is provided with a circumferential array of clamping holes, a bottom plate is placed on the upper end of the rotating disk, and the lower end of the bottom plate is fixedly provided with a circumferential array of clamping columns, and the clamping columns cooperate with the clamping holes.

[0007] Preferably, in order to achieve two-stage ultrafiltration and thus improve the effect of ultrafiltration, a clamping ring is fixedly installed on the upper end of the base plate, filter grooves are evenly opened on the clamping ring, molecular filter plates are clamped and installed between the clamping rings, and the molecular filter plates are in close contact with the clamping rings, and a sealing cover is clamped on the upper end of the clamping ring, a clamping groove is opened at the lower end of the sealing cover, the upper end of the clamping ring is clamped in the clamping groove, and a limiting hole is opened in a circumferential array on the upper end of the sealing cover.

[0008] Preferably, in order to fix the cylinder cover to the upper end of the ultrafiltration cylinder and limit the ultrafiltration component at the same time, lock buckles are fixedly installed on the four external sides of the cylinder cover, and the cylinder cover is clamped to the upper end of the ultrafiltration cylinder through the lock buckles. A plane bearing is fixedly installed on the lower end of the cylinder cover, and a clamping plate is fixedly installed on the lower end of the plane bearing. A limiting column is provided in a circumferential array at the lower end of the clamping plate, and the limiting column cooperates with the limiting hole.

[0009] Preferably, in order to drive the lifting plate and the receiving culture dish to rise and fall, so as to facilitate user access, a heat conducting plate is fixedly installed at the lower end of the inner part of the receiving tube, and the receiving assembly includes a fixing ring, and a micro self-locking motor is fixedly installed in a circumferential array at the upper end of the fixing ring, and a lifting threaded rod is fixedly installed at the output end of the micro self-locking motor, and the lower end of the lifting threaded rod extends to the lower end of the fixing ring, and a lifting plate is threadedly sleeved on the lifting threaded rod, and the lifting plate is slidably installed inside the receiving tube, and a receiving culture dish is fixedly installed at the middle position of the upper end of the lifting plate, and the receiving culture dish is located below the connecting hole.

[0010] Preferably, in order to improve the preservation effect and thus reduce the occurrence of substance inactivation, the temperature control component includes a temperature control box, which is fixedly mounted on the lower end of the receiving tube, and a slow-speed motor is fixedly mounted at the middle position of the outer lower end of the temperature control box, and a mounting disk is fixedly mounted on the output end of the slow-speed motor, the mounting disk is located inside the temperature control box, and a circuit board is fixedly mounted on the upper end of the mounting disk, a temperature control rod is fixedly mounted on the circuit board, and the temperature control rod is electrically connected to the circuit board.

[0011] The application of the graded ultrafiltration purification system for cartilage stem cell paracrine substances includes the following steps: Step 1: First, the mounting ring is installed at the external use location through the mounting rod, and then the cartilage stem cell paracrine substance is poured into the interior of the molecular filter plate, and then sealed with the sealing cover. The control motor can drive the rotating disk to rotate through the rotating shaft, thereby completing centrifugal ultrafiltration. The ultrafiltered substance falls onto the receiving component through the connecting hole for storage.

[0012] Step 2: During the storage process, the temperature regulating rod can adjust the temperature inside the receiving tube through the heat conducting plate, thereby preventing the substance in the receiving culture dish from being inactivated due to the external environment, and during the storage process, the slow motor can drive the mounting plate and the temperature regulating rod to rotate slowly, thereby improving the temperature uniformity. When taking it out, the receiving tube can be unscrewed and supported by the support rod. At the same time, the lifting threaded rod is driven to rotate by the micro self-locking motor, thereby driving the lifting plate to rise and fall, thereby lifting the receiving culture dish for user convenience.

[0013] The beneficial effects of the present invention are: 1. The present invention realizes basic ultrafiltration function by installing the ultrafiltration component in the ultrafiltration cartridge, and the ultrafiltration material can fall onto the receiving component through the connecting hole for temporary storage. During the temporary storage process, the storage temperature is adjusted by the rotatable temperature regulating component, the thermal insulation effect is constant and the temperature in the area is uniform, and the storage effect is better.

[0014] 2. The receiving assembly in the present invention can be raised and lowered in the receiving cylinder. When the filtered material on the receiving assembly needs to be taken out, the ultrafiltered material can be taken out quickly and conveniently by simply controlling the lifting of the receiving culture dish in the receiving assembly.

[0015] 3. The ultrafiltration component of the present invention is designed as a detachable structure, which can be easily and quickly disassembled after use, thereby facilitating the cleaning of substances attached to the ultrafiltration component. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of a half-section view of the overall structure of the present invention; Figure 3 It is a structural schematic diagram of the mounting ring in the present invention; Figure 4 Schematic diagram of the installation of the ultrafiltration component of the present invention; Figure 5 A half-section view of the ultrafiltration cartridge of the present invention; Figure 6 Schematic diagram of the explosion of the ultrafiltration component of the present invention; Figure 7 A half-section view of the drum cover of the present invention; Figure 8 A half-section view of the receiving tube and the temperature regulating assembly of the present invention; Figure 9 It is a partial cross-sectional view of the temperature control component in the present invention.

[0017] Explanation of the accompanying symbols: In the figure: 1, mounting ring; 2, separating ring; 3, ultrafiltration cartridge; 4, ultrafiltration assembly; 401, control motor; 402, rotating shaft; 403, rotating disk; 404, snap-fit ​​hole; 405, bottom plate; 406, snap-fit ​​column; 407, snap-fit ​​ring; 408, molecular filter plate; 409, sealing cover; 4010, limiting hole; 5, cartridge cover; 6, connecting hole; 7, receiving cartridge; 8, receiving assembly; 801, fixing ring; 802, Micro self-locking motor; 803, lifting threaded rod; 804, lifting plate; 805, culture dish receiving device; 9, temperature control assembly; 901, temperature control box; 902, slow motor; 903, mounting plate; 904, circuit board; 905, temperature control rod; 10, support rod; 11, sealing gasket; 12, mounting rod; 13, mounting hole; 14, rotating hole; 15, locking buckle; 16, plane bearing; 17, clamping plate; 18, limit column; 19, heat conduction plate. DETAILED DESCRIPTION

[0018] The present invention will be further described below in conjunction with the accompanying drawings: like Figures 1-9 As shown, this embodiment provides a graded ultrafiltration purification system for cartilage stem cell paracrine substances and its application, including a mounting ring 1, a separator ring 2 is fixedly installed inside the mounting ring 1, an ultrafiltration cartridge 3 is fixedly installed on the upper end of the inner part of the mounting ring 1, the ultrafiltration cartridge 3 is located at the upper end of the separator ring 2, and an ultrafiltration component 4 is installed inside the ultrafiltration cartridge 3, a cartridge cover 5 is installed on the upper end of the ultrafiltration cartridge 3, the cartridge cover 5 is located at the upper end of the ultrafiltration component 4, a connecting hole 6 is opened in a circumferential array at the lower end of the inner part of the ultrafiltration cartridge 3, a receiving cartridge 7 is threadedly installed on the lower end of the inner part of the mounting ring 1, a lifting receiving component 8 is installed inside the receiving cartridge 7, and the receiving cartridge 7 is provided with a lifting receiving component 8. A temperature control component 9 is installed at the lower end of the exterior, and a support rod 10 is fixedly installed in a circumferential array at the lower end of the temperature control component 9. When in use, the structure is first placed at the use location, and then the cartilage stem cell paracrine substance is placed in the ultrafiltration component 4, which is then fixed and sealed by the cylinder cover 5. The ultrafiltration component 4 can perform ultrafiltration, and the ultrafiltered substance will enter the side wall of the ultrafiltration cylinder 3, and under the action of gravity, it will fall through the connecting hole 6 to the receiving culture dish 805 in the receiving component 8 for temporary storage. During the storage process, the temperature control component 9 can adjust the temperature, thereby improving the storage effect and reducing the occurrence of substance inactivation.

[0019] As a technical optimization solution of the present invention, specifically Figure 3 As shown, sealing gaskets 11 are fixedly installed on the upper and lower ends of the separating ring 2, the outer lower end of the ultrafiltration cylinder 3 and the upper end of the receiving cylinder 7 are in close contact with the sealing gasket 11, and the outer array of the mounting ring 1 is fixedly installed with a mounting rod 12. The shape of the mounting rod 12 is L-shaped, and the other end of the mounting rod 12 is provided with a mounting hole 13 in a circumferential array. When in use, first use external bolts to fix this structure to the external use location through the mounting hole 13.

[0020] As a technical optimization solution of the present invention, specifically Figure 3 、 Figure 4 、 Figure 5 and Figure 6 As shown, a cross-shaped rotating hole 14 is provided at the middle position of the lower end of the ultrafiltration cartridge 3, and the ultrafiltration component 4 includes a control motor 401, and a rotating shaft 402 is fixedly installed at the output end of the control motor 401, and the rotating shaft 402 is rotatably clamped in the rotating hole 14, and a rotating disk 403 is fixedly installed on the upper end of the rotating disk 403, and a clamping hole 404 is provided in a circumferential array at the upper end of the rotating disk 403. A bottom plate 405 is placed on the upper end of the rotating disk 403, and a clamping column 406 is fixedly installed in a circumferential array at the lower end of the bottom plate 405, and the clamping column 406 is matched with the clamping hole 404. A clamping ring 407 is fixedly installed on the upper end of the bottom plate 405, and filter grooves are evenly provided on the clamping ring 407. Molecular filter plates 408 are clamped and installed between the clamping rings 407, and the molecular filter plates 408 are in close contact with the clamping rings 407. A sealing cover is clamped on the upper end of the clamping ring 407. 409. A card slot is provided at the lower end of the sealing cover 409. The upper end of the clamping ring 407 is clamped in the card slot. A limiting hole 4010 is provided in a circumferential array at the upper end of the sealing cover 409. Lock buckles 15 are fixedly installed on the four external sides of the cylinder cover 5. The cylinder cover 5 is clamped to the upper end of the ultrafiltration cylinder 3 through the lock buckle 15. A plane bearing 16 is fixedly installed at the lower end of the cylinder cover 5. A clamping plate 17 is fixedly installed at the lower end of the plane bearing 16. A limiting column 18 is provided in a circumferential array at the lower end of the clamping plate 17. The limiting column 18 cooperates with the limiting hole 4010. When in use, the cartilage stem cell paracrine substance is poured into the interior of the molecular filter plate 408, and then sealed by the sealing cover 409. The control motor 401 can drive the rotating disk 403 to rotate through the rotating shaft 402, thereby completing centrifugal ultrafiltration. The ultrafiltered substance falls onto the receiving component 8 through the connecting hole 6 for storage.

[0021] As a technical optimization solution of the present invention, specifically Figure 7 and Figure 8 As shown, the temperature control component 9 includes a temperature control box 901, which is fixedly mounted on the lower end of the receiving tube 7, and a slow motor 902 is fixedly mounted in the middle position of the lower end of the outer portion of the temperature control box 901, and a mounting disk 903 is fixedly mounted on the output end of the slow motor 902, the mounting disk 903 is located inside the temperature control box 901, and a circuit board 904 is fixedly mounted on the upper end of the mounting disk 903, and a temperature control rod 905 is fixedly mounted on the circuit board 904, and the temperature control rod 905 is electrically connected to the circuit board 904. During the storage process, the temperature control rod 905 can adjust the temperature inside the receiving tube 7 through the heat conducting plate 19, thereby preventing the substance in the receiving culture dish 805 from being inactivated due to the external environment, and during the storage process, the slow motor 902 can drive the mounting disk 903 and the temperature control rod 905 to rotate slowly, thereby improving the uniformity of the temperature.

[0022] As a technical optimization solution of the present invention, specifically Figure 7 As shown, a heat conducting plate 19 is fixedly installed at the lower end of the receiving tube 7, and the receiving assembly 8 includes a fixing ring 801, and a micro self-locking motor 802 is fixedly installed at the circumferential array at the upper end of the fixing ring 801, and a lifting threaded rod 803 is fixedly installed at the output end of the micro self-locking motor 802, and the lower end of the lifting threaded rod 803 extends to the lower end of the fixing ring 801, and a lifting plate 804 is threadedly sleeved on the lifting threaded rod 803, and the lifting plate 804 is slidably installed inside the receiving tube 7, and a receiving culture dish 805 is fixedly installed at the middle position of the upper end of the lifting plate 804, and the receiving culture dish 805 is located below the connecting hole 6. When taking it, the receiving tube 7 can be unscrewed and supported by the support rod 10, and at the same time, the lifting threaded rod 803 is driven to rotate by the micro self-locking motor 802, thereby driving the lifting plate 804 to rise and fall, thereby lifting the receiving culture dish 805, which is convenient for users to take it.

[0023] The application of the graded ultrafiltration purification system for cartilage stem cell paracrine substances includes the following steps: Step 1: First, install the mounting ring 1 at the external use location through the mounting rod 12, then pour the cartilage stem cell paracrine substance into the interior of the molecular filter plate 408, and then seal it with the sealing cover 409. The control motor 401 can drive the rotating disk 403 to rotate through the rotating shaft 402, thereby completing centrifugal ultrafiltration. The ultrafiltered substance falls onto the receiving component 8 through the connecting hole 6 for storage.

[0024] Step 2: During the storage process, the temperature regulating rod 905 can adjust the temperature inside the receiving tube 7 through the heat conducting plate 19, thereby preventing the substance in the receiving culture dish 805 from being inactivated due to the external environment, and during the storage process, the slow motor 902 can drive the mounting plate 903 and the temperature regulating rod 905 to rotate slowly, thereby improving the temperature uniformity. When taking it out, the receiving tube 7 can be unscrewed and supported by the support rod 10. At the same time, the micro self-locking motor 802 drives the lifting threaded rod 803 to rotate, thereby driving the lifting plate 804 to rise and fall, thereby lifting the receiving culture dish 805, making it convenient for users to take it out.

[0025] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which shall fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A graded ultrafiltration purification system for cartilage stem cell paracrine substances, comprising a mounting ring (1), characterized in that: A separator ring (2) is fixedly installed inside the mounting ring (1), an ultrafiltration cartridge (3) is fixedly installed on the upper end of the inner part of the mounting ring (1), the ultrafiltration cartridge (3) is located at the upper end of the separator ring (2), and an ultrafiltration assembly (4) is installed inside the ultrafiltration cartridge (3), a cartridge cover (5) is installed on the upper end of the ultrafiltration cartridge (3), the cartridge cover (5) is located at the upper end of the ultrafiltration assembly (4), a connecting hole (6) is provided in a circumferential array at the lower end of the inner part of the ultrafiltration cartridge (3), a receiving cartridge (7) is threadedly installed on the lower end of the inner part of the mounting ring (1), a lifting receiving assembly (8) is installed inside the receiving cartridge (7), a temperature control assembly (9) is installed on the outer lower end of the receiving cartridge (7), and a support rod (10) is fixedly installed in a circumferential array at the lower end of the temperature control assembly (9).

2. The system for fractionated ultrafiltration purification of cartilage stem cell paracrine substances according to claim 1, characterized in that: Sealing rings (11) are fixedly mounted on both upper and lower ends of the separation ring (2); the outer lower end of the ultrafiltration cylinder (3) and the upper end of the receiving cylinder (7) are in close contact with the sealing ring (11); a mounting rod (12) is fixedly mounted on the outer array of the mounting ring (1); the mounting rod (12) is L-shaped, and a mounting hole (13) is provided in a circumferential array at the other end of the mounting rod (12).

3. The system for fractionated ultrafiltration purification of cartilage stem cell paracrine substances according to claim 1, characterized in that: A cross-shaped rotating hole (14) is provided at the middle position of the lower end of the ultrafiltration cartridge (3), and the ultrafiltration component (4) includes a control motor (401). A rotating shaft (402) is fixedly installed at the output end of the control motor (401), and the rotating shaft (402) is rotatably engaged in the rotating hole (14). A rotating disk (403) is fixedly installed at the upper end of the rotating shaft (402), and engaging holes (404) are provided in a circumferential array at the upper end of the rotating disk (403). A bottom plate (405) is placed at the upper end of the rotating disk (403), and engaging posts (406) are fixedly installed in a circumferential array at the lower end of the bottom plate (405), and the engaging posts (406) are matched with the engaging holes (404).

4. The system for fractionated ultrafiltration purification of cartilage stem cell paracrine substances according to claim 3, characterized in that: A clamping ring (407) is fixedly mounted on the upper end of the base plate (405), and filter grooves are evenly provided on the clamping ring (407). A molecular filter plate (408) is clamped and mounted between the clamping rings (407), and the molecular filter plate (408) is in close contact with the clamping ring (407). A sealing cover (409) is clamped on the upper end of the clamping ring (407), and a clamping groove is provided on the lower end of the sealing cover (409). The upper end of the clamping ring (407) is clamped in the clamping groove, and a limiting hole (4010) is provided in a circumferential array on the upper end of the sealing cover (409).

5. The graded ultrafiltration purification system for cartilage stem cell paracrine substances according to claim 4, characterized in that: Lock buckles (15) are fixedly installed on the four outer sides of the cylinder cover (5), and the cylinder cover (5) is clamped to the upper end of the ultrafiltration cylinder (3) through the lock buckles (15). A plane bearing (16) is fixedly installed on the lower end of the cylinder cover (5), and a clamping plate (17) is fixedly installed on the lower end of the plane bearing (16). A limiting column (18) is arranged in a circumferential array at the lower end of the clamping plate (17), and the limiting column (18) cooperates with the limiting hole (4010).

6. The system for fractionated ultrafiltration purification of cartilage stem cell paracrine substances according to claim 1, characterized in that: A heat conducting plate (19) is fixedly mounted on the lower end of the receiving tube (7), and the receiving assembly (8) includes a fixing ring (801), a micro self-locking motor (802) is fixedly mounted in a circumferential array on the upper end of the fixing ring (801), and a lifting threaded rod (803) is fixedly mounted on the output end of the micro self-locking motor (802), the lower end of the lifting threaded rod (803) extends to the lower end of the fixing ring (801), and a lifting plate (804) is threadedly sleeved on the lifting threaded rod (803), and the lifting plate (804) is slidably mounted inside the receiving tube (7), and a receiving culture dish (805) is fixedly mounted at the middle position of the upper end of the lifting plate (804), and the receiving culture dish (805) is located below the connecting hole (6).

7. The system for fractionated ultrafiltration purification of cartilage stem cell paracrine substances according to claim 1, characterized in that: The temperature control assembly (9) includes a temperature control box (901), the temperature control box (901) is fixedly mounted on the lower end of the receiving tube (7), a slow motor (902) is fixedly mounted at the middle position of the lower end of the outside of the temperature control box (901), a mounting plate (903) is fixedly mounted on the output end of the slow motor (902), the mounting plate (903) is located inside the temperature control box (901), and a circuit board (904) is fixedly mounted on the upper end of the mounting plate (903), a temperature control rod (905) is fixedly mounted on the circuit board (904), and the temperature control rod (905) is electrically connected to the circuit board (904).

8. Use of the graded ultrafiltration purification system for cartilage stem cell paracrine substances according to any one of claims 1 to 7 in the treatment of cartilage stem cell paracrine substances, characterized in that: The following steps are involved: Step 1: First, the mounting ring (1) is installed at the external use location through the mounting rod (12), and then the cartilage stem cell paracrine substance is poured into the interior of the molecular filter plate (408), and then sealed through the sealing cover (409). The control motor (401) can drive the rotating disk (403) to rotate through the rotating shaft (402), thereby completing centrifugal ultrafiltration. The ultrafiltered substance falls onto the receiving component (8) through the connecting hole (6) for storage. Step 2: During the storage process, the temperature regulating rod (905) can adjust the temperature inside the receiving tube (7) through the heat conducting plate (19), thereby preventing the substance in the receiving culture dish (805) from being inactivated due to the external environment, and during the storage process, the slow motor (902) can drive the mounting plate (903) and the temperature regulating rod (905) to rotate slowly, thereby improving the uniformity of temperature. When taking out, the receiving tube (7) can be unscrewed and supported by the support rod (10), and at the same time, the lifting threaded rod (803) can be driven to rotate by the micro self-locking motor (802), thereby driving the lifting plate (804) to rise and fall, thereby lifting the receiving culture dish (805) to facilitate the user to take out.

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