Mite-killing composition based on sophorolipid and inula helianthus extract and application of mite-killing composition

Abstract

The invention relates to the field of daily chemicals, and discloses an acarus killing composition based on sophorolipid and an inula helianthus extract and application of the acarus killing composition. The acarus killing composition comprises the following components in percentage by weight in cosmetics or wash supplies: 0.5 to 20 percent of sophorolipid and 0.1 to 3 percent of water inula flower extract. According to the invention, the sophorolipid and the water-containing inula flower extract can generate a synergistic effect after being compounded, so that the acarus killing effect is remarkably improved. Besides, the acarus killing composition is simple in formula and capable of reducing the content of components such as high-irritation surfactants and the like, so that the acarus killing composition has the characteristics of mild property and low irritation and is more suitable for being applied to the fields of cosmetics or wash supplies and the like.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a mite removal composition based on sophorolipids and an Inula japonica flower extract and an application thereof. Background Art

[0002] Demodex, commonly known as follicle mites, belongs to the genus Demodex of the family Demodexidae. Human Demodex mites are milky white and translucent, and are permanent external parasites of mammals. Depending on the location of their infestation, they are divided into two types: Demodex folliculorum and Demodex brevis. They primarily inhabit areas of the face with well-developed sebaceous glands, such as the forehead, nose tip, nasolabial folds, and cheeks. Demodex mites feed on cellular metabolites such as sebum. Excessive reproduction can damage sebaceous glands and hair follicles, causing clogged and dilated hair follicles, rough and dry epidermis, and the development of red papules, nodules, and cysts. In severe cases, secondary bacterial infections can lead to lesions such as folliculitis, pustules, or inflammatory cysts. Demodex infection rates are high in all age groups, reaching over 90%, with oily skin being particularly susceptible, particularly in spring and summer. Demodectic diseases, such as seborrheic alopecia, acne, and seborrheic dermatitis caused by Demodex folliculorum, and erythema, nodules, and rosacea caused by Demodex sebaceum, seriously affect the patient's skin health and cause great physical and psychological harm to the patient.

[0003] Natural active substances are favored by consumers for their antibacterial, mite removal, and anti-inflammatory effects. Adding effective natural active substances has gradually become a new trend in the development of daily chemical products. Sophorolipids, as a green biosurfactant, have unique physical and chemical properties. Sophorolipids have good emulsifying properties and surface activity, can reduce oil-water interfacial tension, and have excellent surface activity. In addition, sophorolipids also have certain antibacterial and anti-inflammatory biological activities. They can be used as emulsifiers, moisturizers, antibacterial agents, etc. in daily chemical products such as daily detergents and skin care, showing the characteristics of safety, mildness, low irritation, and non-toxicity. Some research articles and patents show that sophorolipids also have a certain mite removal effect, but their mite removal effect is not ideal and needs to be further improved.

[0004] Inula strychnifolia is a perennial herbaceous plant of the genus Inula in the Asteraceae family. It is mainly distributed in Yunnan, Sichuan, southern Gansu and western Guizhou in my country. The "Compendium of Chinese Materia Medica" records that its inflorescence or whole plant tastes fishy, ​​salty, slightly bitter, and cool in nature, and has the functions of lowering qi, promoting water circulation, and removing phlegm. Inula strychnifolia contains polysaccharides, flavonoids and terpenoids, etc., and has significant anti-tumor and other biological activities. Patent CN 102000066 A discloses an extract of a mixed crystal of sesquiterpene lactone and chrysanthemin in an extract of Inula strychnifolia, and shows that it has good anti-tumor application prospects. However, there is no report that it has a mite removal effect on its own or can have a synergistic mite removal effect with other substances.

[0005] Although there have been reports on mite removal schemes based on a single natural plant extract or a combination of multiple plant extracts, such as patent CN 116602879 A discloses a mite removal composition comprising 4-terpineol, menthol, borneol, artificial bezoar, Mayinglong raw powder, Litsea cubeba fruit oil, clove bud extract, thyme extract and neem seed extract, which has both mite removal and antipruritic effects; patent CN 118787574 A discloses a shower gel comprising dipotassium glycyrrhizate, arbutin, sulfur, and extracts from multiple plants such as Platycladus orientalis, Polygonum multiflorum and Artemisia annua, which has mite removal, antipruritic and whitening effects; patent CN115414295 A provides a compound plant anti-acne and anti-mite composition composed of five plant extracts from Scutellaria baicalensis root, Salvia miltiorrhiza, Coptis chinensis, Centella asiatica and Gleditsia thorn. However, the compositions or formulas provided in these patents often use irritating sulfate surfactants and sulfur for cleaning and mite removal, or the plant-based ingredients are complex and have unclear mechanisms of action. There is a lack of effective mite removal compositions and products that are both mild and low-irritating, with simple ingredients and clear mechanisms. Therefore, there is an urgent need to develop a mild, low-irritation composition formula that can also achieve mite removal effectiveness for use in personal care products such as facial cleansers, shampoos, face creams, and body washes. Summary of the Invention

[0006] To address the above technical problems, the present invention provides a mite removal composition based on a sophorolipid biosurfactant and an extract of Inula japonica flower, and its application. The present invention discovered that the combination of sophorolipids and an extract of Inula japonica flower can produce a synergistic effect, thereby significantly improving the mite removal effect. Furthermore, the mite removal composition of the present invention has a simple formula, which can reduce the content of highly irritating ingredients such as surfactants. Therefore, it has the characteristics of mild properties and low irritation, making it more suitable for applications in cosmetics, toiletries, and other fields.

[0007] The specific technical solutions of the present invention are: In a first aspect, the present invention provides a mite removal composition based on sophorolipids and Inula japonica extract, wherein the content of the composition in cosmetics or toiletries is as follows: 0.5-20% sophorolipids and 0.1-3% Inula japonica extract, calculated by mass percentage.

[0008] The extract of Inula helianthus-aquatica CYWu ex Ling is rich in flavonoids, terpenes and other substances. The present invention is the first to discover that when sophorolipids are compounded with the extract of Inula helianthus-aquatica CYWu ex Ling according to the above-mentioned specific content, the two can produce a synergistic effect, which can significantly improve the mite removal effect compared with a single sophorolipid.

[0009] In addition, in addition to its mite removal effect, the sophorolipid in the composition of the present invention can also act as an emulsifier, moisturizer, and surfactant, which can kill mites while cleaning skin oil and mite corpses. That is, the sophorolipid plays a dual role of mite removal and surfactant at the same time. Therefore, its presence can reduce the amount of other highly irritating ingredients such as surfactants in the product, making the product mild and low in irritation, making it more suitable for use in cosmetics or toiletries.

[0010] Preferably, the content of the sophorolipid is 1-15%, and the content of the Inula fragrans extract is 0.1-2.5%; further preferably, the content of the sophorolipid is 2-10%, and the content of the Inula fragrans extract is 0.5-2%.

[0011] The present invention has found that within the above concentration range, the composition can exert a good effect in removing mites.

[0012] Preferably, the sophorolipid is one or a combination of acid-type sophorolipid and lactone-type sophorolipid.

[0013] Preferably, the Inula helianthus-aquatica CYWu ex Ling extract refers to an extract of the dried inflorescence of Inula helianthus-aquatica CYWu ex Ling.

[0014] Preferably, the preparation method of the Inula fuchsia extract comprises: 1) Cleaning and drying: clean the columnar inflorescence of Inula japonica L. with fresh water and dry it; 2) Grinding and sieving: Grind the flower heads of Inula shuichaoyangensis and then sieve; 3) Extraction: The obtained Inula japonica powder is mixed with an ethanol aqueous solution at a solid-liquid ratio of 1 / 10-1 / 40 g / mL, and reflux extraction is performed. The obtained extract is concentrated under reduced pressure, extracted, and the aqueous phase is collected. After removing the solvent, the obtained extract is the Inula japonica extract.

[0015] Preferably, in step 1), the drying method is one or a combination of natural air drying, oven drying, vacuum drying, and vacuum freeze drying. Preferably, the drying method is one or more of vacuum drying and vacuum freeze drying.

[0016] Preferably, in step 2), the powder is collected after being sieved through a 30-50 mesh sieve.

[0017] Preferably, in step 3), the concentration of the ethanol aqueous solution is 70%-100%; more preferably 80%-100%; and even more preferably 85%-95%.

[0018] Preferably, in step 3), the solid-to-liquid ratio is 1:15-1:30; most preferably 1:20.

[0019] Preferably, in step 3), the number of reflux extractions is 1-6 times; preferably 2-5 times; and most preferably 3 times.

[0020] Preferably, in step 3), ethyl acetate is used for the extraction, and the number of extractions is 1-3 times.

[0021] In a second aspect, the present invention provides the use of a mite removal composition based on sophorolipids and an Inula chinensis flower extract in the preparation of cosmetics or toiletries.

[0022] Preferably, the mite removal composition is prepared together with a solvent, carrier or excipient acceptable to cosmetics or toiletries to prepare cosmetics or toiletries with mite removal efficacy.

[0023] Preferably, the cosmetics or toiletries are creams, lotions, solutions, or films, for example, face creams, skin care lotions, shampoos, shower gels, facial masks, eye patches, and the like.

[0024] Compared with the prior art, the present invention has the following beneficial effects: (1) The composition of the present invention contains both sophorolipids and Inula japonica extract, and the synergistic effect of the two makes the composition have an excellent mite removal effect.

[0025] (2) Since sophorolipids have good emulsifying, solubilizing, and dispersing properties, and have certain surface activity, they are mild and low in irritation. When used in cosmetics or toiletries, they can reduce the irritation of traditional surfactants or emulsifiers. Therefore, they are particularly suitable for addition to cosmetics and toiletries designed for sensitive skin and sensitive areas such as the eyes. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1This is a statistical chart of the mite removal effects of different sophorolipid-Inula fragrans extract compositions; Figure 2 The results show the effects of different sophorolipid-Inula fragrans extract compositions on NO release in RAW264.7 cells; Figure 3 These are the results of the effects of different sophorolipid-inula flavescentis extract compositions on the release of inflammatory factor IL-6 from RAW264.7 cells. DETAILED DESCRIPTION

[0027] In order to facilitate the understanding of those skilled in the art, the present invention is further described below in conjunction with several preferred embodiments and test results of the present invention. It should be noted that the following embodiments are not limitations of the present invention, and any changes or modifications to the present invention will fall within the scope of protection of the present invention.

[0028] First, a mite removal composition based on sophorolipids and Inula japonica flower extract is provided, wherein the content of the composition in cosmetics or toiletries is as follows: 0.5-20% sophorolipids and 0.1-3% Inula japonica flower extract, calculated by mass percentage.

[0029] Preferably, the content of the sophorolipid is 1-15%, and the content of the Inula fragrans extract is 0.1-2.5%; further preferably, the content of the sophorolipid is 2-10%, and the content of the Inula fragrans extract is 0.5-2%.

[0030] Preferably, the sophorolipid is one or a combination of acid-type sophorolipid and lactone-type sophorolipid.

[0031] Preferably, the Inula helianthus-aquatica CYWu ex Ling extract refers to an extract of the dried inflorescence of Inula helianthus-aquatica CYWu ex Ling.

[0032] Preferably, the preparation method of the Inula fuchsia extract comprises: 1) Cleaning and drying: clean the columnar inflorescence of fresh water Inula japonica and dry it; 2) Grinding and sieving: Grind the flower heads of Inula shuichaoyangensis and then sieve; 3) Extraction: The obtained Inula japonica powder is mixed with an ethanol aqueous solution at a solid-liquid ratio of 1 / 10-1 / 40 g / mL, and reflux extraction is performed. The obtained extract is concentrated under reduced pressure, extracted, and the aqueous phase is collected. After removing the solvent, the obtained extract is the Inula japonica extract.

[0033] Preferably, in step 1), the drying method is one or a combination of natural air drying, oven drying, vacuum drying, and vacuum freeze drying. Preferably, the drying method is one or more of vacuum drying and vacuum freeze drying.

[0034] Preferably, in step 2), the powder is collected after being sieved through a 30-50 mesh sieve.

[0035] Preferably, in step 3), the concentration of the ethanol-water solution is 70%-100%, more preferably 80%-100%, and even more preferably 85%-95%. The solid-to-liquid ratio is 1:15-1:30, and most preferably 1:20. The reflux extraction is performed 1-6 times, preferably 2-5 times, and most preferably 3 times. The extraction is performed using ethyl acetate, and the number of extractions is 1-3.

[0036] Secondly, a cosmetic or toiletries containing the above mite removal composition.

[0037] Preferably, the mite removal composition is prepared together with a solvent, carrier or excipient acceptable to cosmetics or toiletries to prepare cosmetics or toiletries with mite removal efficacy.

[0038] Preferably, the cosmetics or toiletries are creams, lotions, solutions, or films, for example, face creams, skin care lotions, shampoos, shower gels, facial masks, eye patches, and the like.

[0039] Example 1: Preparation of Inula japonica Flower Extract The preparation method of the Inula japonica extract is as follows: (1) Cleaning and drying: Take fresh columnar inflorescences of Inula japonica Thunb., clean them, drain the water, and then use vacuum freeze drying to completely dry the Inula japonica Thunb.

[0040] (2) Grinding and sieving: Grind the completely dried inflorescence of Inula japonica L. and pass it through a sieve. Collect the powder collected after sieving through a 40-mesh sieve and set aside.

[0041] (3) Extraction: Mix the powder of Inula japonica Thunb. with 90% ethanol aqueous solution at a solid-liquid ratio of 1 g:20 mL, and reflux extraction for 3 times, each time for 2.5 hours; combine the extracts, concentrate under reduced pressure, and remove most of the solvent.

[0042] (4) Extraction: The extract was thoroughly mixed with ethyl acetate, then allowed to stand and the aqueous phase was collected. The extraction was repeated twice, and the aqueous phase was collected. After removing most of the solvent, the extract obtained was the extract of Inula japonica L.

[0043] Example 2-10: Preparation of Sophorolipid-Inula fragrans Extract Composition As shown in Table 1, a combination scheme of sophorolipids and Inula japonica extract is shown, and the percentages shown are weight percentages. Sophorolipids were purchased from Nanjing Xuankai Biotechnology Co., Ltd., and Inula japonica extract was extracted according to the method of Example 1.

[0044] The preparation method is as follows: a certain amount of sophorolipids and water inula fragrans extract are mixed, water is added to make the final concentration reach the final concentration shown in Example 2-10 in Table 1, heated at 50°C while stirring to mix thoroughly, and cooled to room temperature for use.

[0045] Table 1: Content of the sophorolipid-Inula fragrans extract composition in Examples 2-10 Comparative Examples 1-3: The preparation method only contains sophorolipids at different concentrations and does not contain Inula japonica extract (as shown in Table 2). The specific concentration and preparation method are the same as those in the embodiment.

[0046] Comparative Examples 4-6: The preparations only contain different concentrations of Inula japonica flower extract, but do not contain sophorolipids (as shown in Table 2). The specific concentrations and preparation methods are the same as those in the examples.

[0047] Table 2: Contents of sophorolipids and Inula japonica extract in Comparative Examples 1-6 Performance Testing The mite removal and anti-inflammatory effects of the sophorolipid-Inula fragrans complex were evaluated using an in vitro Demodex growth inhibition experiment and an LPS-induced mouse mononuclear macrophage RAW264.7 inflammation model. The specific test methods are as follows: (1) In vitro mite removal test materials: stereo microscope, artificial climate chamber, RPMI 1640 culture medium, transparent tape, pure water, micropipette, slide, etc.

[0048] Test mite type: Human Demodex mites were collected from the patient's face using the transparent tape method.

[0049] Test method: The mite removal test was conducted by making a simple modification to the mite removal method provided in the group standard "Evaluation Method for Mite Repellent, Mite Suppressant and Mite Killing Performance of Daily Chemical Products" (T / CHCIA002-2022). The method is briefly described as follows: (1) Obtaining human Demodex mites: The day before collecting mites, volunteers clean their faces and then apply transparent tape to the forehead, cheeks, nose, chin, and other parts of the face, gently pressing. The next day, the tape is gently removed, and the obtained Demodex mites are confirmed by microscopic examination and transferred to RPMI 1640 culture medium and stored at 4°C until ready for use.

[0050] (2) 200 μL of different concentrations of sophorolipid biosurfactant-Inula japonica extract compositions were pipetted onto a glass slide using a micropipette. After spreading evenly, Demodex mites were placed on the slide. The slide was then transferred to an artificial climate chamber and incubated at 28°C and 75% humidity for 4 hours. The survival of the mites was then observed under a microscope. Pure water was used as a blank control group. In the experiment, 30 Demodex mites were tested under each condition, and the test was repeated three times in parallel. The final mite removal rate was averaged.

[0051] The criteria for judging the death of mites are as follows: observe continuously for 1 minute under a 400x microscope. If the chelicerae or claws of the Demodex mite do not move, it can be preliminarily judged to be dead; then incubate at room temperature for 30 minutes, observe the claws again for 1 minute, and if there is still no movement, the mites are determined to be dead.

[0052] (3) The calculation method of Demodex mortality rate is as follows: Corrected mortality rate = [(number of surviving insects in the blank control group - number of surviving insects in the experimental group) / number of surviving insects in the blank control group] × 100%.

[0053] The mite removal effects of Examples 2-10 and Comparative Examples 1-6 are shown in Table 3.

[0054] Table 3: Mite removal effect According to the data results in Table 3, we can see that: The mite removal rates of the samples containing only sophorolipids in Comparative Examples 1 to 3 were all greater than 80%, and as the concentration of sophorolipids increased, the mite removal rate also increased, indicating that the sophorolipids as a single active ingredient themselves have a certain mite removal effect. The samples of Comparative Examples 4 to 6 contained only the extract of Inula japonica, and the results showed that their mite removal rates were very low.

[0055] In Examples 2 to 4, the concentration of sophorolipids was the same, and the concentration of Inula japonica extract gradually increased, and the mite removal rates were all above 89%, and were higher than those in Comparative Example 1. Figure 1 As shown, the mite removal efficiency of the combination is significantly higher than that of using either one alone; in addition, the sum of the mite removal rates of using 2% sophorolipids or 1% water Inula fragrans extract alone (85.4%) is lower than the mite removal efficiency when the two are used together (89.4%), indicating that there is a synergistic effect between the two. Similarly, the mite removal efficiency of the samples of Examples 5-7 is higher than that of Comparative Example 2, and the mite removal rates of Examples 8-10 are higher than those of Comparative Example 3.

[0056] In summary, the results show that a single sophorolipid has a certain mite removal effect. On this basis, the addition of Inula scabra extract can significantly improve the mite removal efficiency of sophorolipid, and the two have a synergistic effect.

[0057] (II) LPS-induced inflammation model experiment in macrophage RAW264.7 cells Main experimental materials: mouse mononuclear macrophage leukemia cells RAW264.7 (from Wuhan Punosai Life Science Technology Co., Ltd.); high-glucose DMEM culture medium, fetal bovine serum, PBS and trypsin (all from Gibco); mouse inflammatory factor IL-6 ELISA kit, NO detection kit and CCK-8 detection kit (from Beyotime).

[0058] The test method is as follows: (1) Culture of RAW264.7 cells: Cells in the logarithmic growth phase were taken and inoculated into each well of a 24-well plate at 0.5 × 10 5 The cells were cultured in an incubator containing 5% CO2 at 37°C and used for cytotoxicity detection or LPS-induced inflammation model establishment after 24 hours.

[0059] (2) The cytotoxicity of the sophorolipid-Inula japonica extract composition was detected using the CCK-8 method to evaluate the cytotoxicity of the sophorolipid-Inula japonica extract composition under different concentrations and ratios. The wells without the composition were used as blank controls, 1 μg / mL LPS was used as the modeling condition (model group), and the composition in Table 1 was used as the experimental group. Three replicate wells were set up for each group.

[0060] (3) LPS-induced inflammation model: The experiment set up a blank control group (no LPS, only DMEM culture medium), a model group (only LPS and DMEM culture medium were added, and no sophorolipid-water Chaoyang Inula flower extract composition was added), and an experimental group. The experimental group includes Examples 2 to 7 and Comparative Examples 1 to 6 listed in Table 1. In the experiment, the cell wells cultured in the first step were added with corresponding LPS or a mixture of sophorolipid composition and LPS according to the blank group, model group, and experimental group. Three replicate wells were set for each group, and the cells were cultured for 24 hours before being used for inflammatory factor testing. In the experiment, the LPS concentration was 1 μg / mL.

[0061] (4) Detection of inflammatory factor IL-6 and NO release levels: According to the operating instructions of the Biyuntian ELISA detection kit and the NO detection kit, the effects of the sophorolipid-inula fragrans extract composition on the release levels of cellular inflammatory factors and NO release levels were detected to evaluate the anti-inflammatory efficacy of the sophorolipid-inula fragrans extract composition.

[0062] The toxicity test results of the sophorolipid-Inula fragrans composition on macrophage RAW264.7 are shown in Table 4.

[0063] Table 4: Cytotoxicity test results of Examples 2-7 and Comparative Examples 1-6 on RAW264.7 As can be seen from Table 4, whether it is sophorolipids alone, Inula japonica extract, or a combination of the two, the survival rate of mouse macrophages RAW264.7 is slightly affected. The survival rate of RAW264.7 cells under the condition of 1μg / mL LPS is greater than 90%. The above results show that 1μg / mL LPS can be used as an inflammatory induction condition for macrophages RAW264.7; 5% sophorolipids and their combination with 0.5%-2% Inula japonica extract have little effect on the survival rate of RAW264.7 cells and have no obvious cytotoxicity.

[0064] Table 5 shows the level of NO released by RAW264.7 cells under different composition conditions. The bar graph is drawn from the data in Table 5. Figure 2 .

[0065] Table 5: NO release from RAW264.7 cells induced by LPS under the conditions of Examples 2-7 and Comparative Examples 1-6 Observe Table 5 and Figure 2 It can be seen that, first, after induction with 1 μg / mL LPS, the amount of NO released by the cells was significantly higher than that of the blank control group, indicating that the model was successfully established. For either the single sophorolipid or the water inula flower extract, it also had the effect of reducing the amount of NO released, and showed a concentration-dependent effect. When the sophorolipid and the water inula flower extract were combined, the NO level was lower than when either was used alone, indicating that there was a synergistic effect between the sophorolipid and the water inula flower extract.

[0066] Similarly, the level of inflammatory factor IL-6 in RAW264.7 cells under the same experimental conditions was detected. The results are summarized in Table 6 and plotted as a bar graph as shown in Figure 3 shown.

[0067] Table 6: Expression of inflammatory factor IL-6 in RAW264.7 cells induced by LPS under the conditions of Examples 2-7 and Comparative Examples 1-6 From Table 6 and Figure 3It can be seen that, similar to the NO level, after 1 μg / mL LPS induction, the content of inflammatory factor IL-6 in RAW264.7 cells was significantly higher than that in the blank control group, indicating that the modeling was successful. Secondly, both the sophorolipids and the water Chaoyang Inula flower extract composition have a certain ability to reduce the expression of the inflammatory factor IL-6, and the IL-6 level of the water Chaoyang Inula flower extract group (Comparative Example 4-Comparative Example 6) is lower, indicating that both have a certain anti-inflammatory ability, and the anti-inflammatory ability of the water Chaoyang Inula flower extract is better than that of sophorolipids. Most importantly, when the two are combined together, the cell IL-6 release level is lower, indicating that the anti-inflammatory effect of the composition is better than that of the water Chaoyang Inula flower extract alone, that is, there is a certain synergistic effect between the two.

[0068] Application Example 1: A shampoo containing a composition of sophorolipids and an Inula chinensis flower extract, the formula of which is shown in Table 7.

[0069] Table 7 Components Raw material name Content (mass percentage, %) 1 water Replenish to 100 2 Polyquaternium-10 0.1 3 Hydroxypropyl guar trimethylammonium chloride 0.4 4 Disodium EDTA 0.1 5 Cocamidopropyl Betaine 3.0 6 Sodium Laureth Sulfate 5.0 7 Sophorolipids 5.0 8 Cocoyl monoethanolamine 1.0 9 Cetearyl Alcohol 0.35 10 Ethylene glycol distearate 1.0 11 Allantoin 0.15 12 Acrylates copolymers 2.0 13 Citric acid / sodium citrate 0.5 14 PEG-14M 0.8 15 Methylchloroisothiazolinone 0.2 16 essence 0.5 17 Polydimethylsiloxyethanol 2.5 18 Polydimethylsiloxane 1.0 19 Inula japonica extract (Example 1) 1.0 The shampoo formula above incorporates 5% sophorolipids and 1% aqueous Inula japonica extract to achieve highly effective worm removal. Furthermore, this formula reduces the use of the traditional surfactant sodium laureth sulfate, using sophorolipids to supplement the surface activity required for cleansing and reduce shampoo irritation. The other ingredients in this formula are common base ingredients found in shampoos.

[0070] Application Example 2: A facial cleanser containing a composition of sophorolipids and an Inula chinensis flower extract, the formula of which is shown in Table 8.

[0071] Table 8 Application Example 3: An emulsion containing a composition of sophorolipids and an Inula japonica flower extract, the formula of which is shown in Table 9: Table 9 Components Raw material name Content (mass percentage, %) 1 water Replenish to 100 2 Sodium Lauryl Sulfate 8.0 3 APG 8.0 4 Sophorolipids 5.0 5 Inula japonica extract (Example 1) 1.0 6 Ethylene glycol stearate 1.0 7 glycerin 2.0 8 Disodium EDTA 0.2 9 citric acid 0.2 10 Sodium chloride 1.5 11 preservative 0.5 12 essence 0.5 Application Example 4: A facial cream containing a composition of sophorolipids and an Inula japonica flower extract, the formula of which is shown in Table 10.

[0072] Table 10 Application Example 1-4: In vitro mite removal effect The same in vitro mite removal efficacy testing method as in Examples 2-10 was used, with the test samples adjusted to the corresponding shampoo, facial cleanser, lotion, and cream in Application Examples 1-4. The mite mortality rate (mite kill rate) was observed and calculated. The statistical results are shown in Table 11.

[0073] Table 11: In vitro mite removal effects of application examples 1-4 In the field of daily chemical products, many functional compositions are easily affected by other components in the product. This means that after adding these functional compositions to a product, some other components in the formula antagonize the functional compositions, preventing them from fully exerting their intended efficacy. However, as shown in Table 11, the mite-killing composition of the present invention, when added to the shampoo, facial cleanser, lotion, and cream of Application Examples 1-4, is virtually unaffected by the other components in the formulations, and the corresponding products still exhibit excellent mite-killing effects.

Claims

1. A mite removal composition based on sophorolipids and Inula japonica extract, characterized by: Calculated by mass percentage, the content of the biosurfactant in cosmetics or toiletries is: 0.5-20% of sophorolipids and 0.1-3% of Inula japonica flower extract.

2. The mite removal composition according to claim 1, wherein: The content of the sophorolipid is 1-15%, and the content of the Inula fragrans extract is 0.1-2.5%.

3. The mite removal composition according to claim 2, wherein: The content of the sophorolipid is 2-10%, and the content of the Inula japonica flower extract is 0.5-2%.

4. The mite removal composition according to any one of claims 1 to 3, characterized in that: The sophorolipid is one or a combination of acid-type sophorolipid and lactone-type sophorolipid.

5. The mite removal composition according to any one of claims 1 to 3, characterized in that: The Inula japonica flower extract refers to an extract of the dried inflorescence of Inula japonica flower.

6. The mite removal composition according to claim 5, wherein: The preparation method of the Inula fuchsia extract comprises: 1) Cleaning and drying: Take fresh water to clean the columnar inflorescence of Inula japonica and dry it; 2) Grinding and sieving: Grind the flower heads of Inula shuichaoyangensis and sieve them; 3) Extraction: The obtained Inula japonica flower powder is mixed with an ethanol aqueous solution at a solid-liquid ratio of 1 / 10-1 / 40 g / mL, and the mixture is refluxed for extraction. The obtained extract is concentrated under reduced pressure, extracted, and the aqueous phase is collected. After removing the solvent, the obtained extract is the Inula japonica flower extract.

7. The mite-killing composition according to claim 6, wherein: In step 3), The concentration of the ethanol aqueous solution is 70%-100%; The solid-liquid ratio is 1:15-1:30; The number of reflux extractions is 1-6 times; The extraction uses ethyl acetate, and the number of extractions is 1-3 times.

8. Use of the mite-killing composition based on sophorolipid and Inula japonica flower extract according to any one of claims 1 to 7 in the preparation of cosmetics or toiletries.

9. The use according to claim 8, characterized in that: The cosmetics or toiletries are creams, lotions, water solutions, and films.

10. Use of an extract of Inula japonica Thunb as a mite removal accelerator in the preparation of a mite removal composition, characterized in that: The mite removal composition comprises sophorolipids and Inula japonica flower extract, and the contents thereof in cosmetics or toiletries are, by mass percentage, 0.5-20% sophorolipids and 0.1-3% Inula japonica flower extract.

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