Preparation method and application of composition of traditional Chinese medicine whitening extracts

Abstract

The invention discloses a preparation method and application of a composition of traditional Chinese medicine whitening extracts, belongs to the technical field of whitening oral products, and particularly relates to a composition of a dendrobium polysaccharide extract, an almond extract, a polygonatum odoratum extract and a smoked plum fermentation extract.The composition has the whitening effect through verification of zebra fish experiments, can be processed into functional health-care food and has a good application prospect. And a new choice is provided for consumers who like whitening.

Description

Technical Field

[0001] The invention belongs to the technical field of oral whitening skin care products, and particularly relates to a preparation method and application of a composition of traditional Chinese medicine whitening extracts. Background Art

[0002] At present, there are many whitening products on the market, most of which are for external use, and many chemical components have certain side effects. Common whitening ingredients, such as kojic acid, hydroquinone, niacinamide, etc., although they can inhibit the accumulation of melanin synthesis, may also cause adverse reactions such as allergies, skin irritation and contact dermatitis, and even have potential carcinogenicity. In traditional Chinese medicine, there are many Chinese medicines with whitening effects. These Chinese medicines can not only achieve the effect of beauty by external application, but also can achieve beauty purposes by oral conditioning of body functions. The present invention is based on the theory of traditional Chinese medicine and combines modern pharmacological efficacy evaluation methods to make an oral product with whitening effect, providing new options for whitening consumers. Summary of the Invention

[0003] The present invention aims to provide a preparation method of a composition of a whitening extract of traditional Chinese medicine and its application.

[0004] The technical solution of the invention mainly includes:

[0005] A method for preparing extracts of dendrobium, bitter almond, polygonatum and black plum, characterized by comprising the following steps:

[0006] (1) Slicing fresh dendrobium strips, freeze-drying, crushing, and sieving to obtain a dendrobium polysaccharide extract;

[0007] (2) roasting the bitter almonds, adding ethanol, and adding β-glucosidase to convert the bitter almonds, distilling to recover the ethanol, and drying to obtain the bitter almond extract;

[0008] (3) crushing the polygonatum, adding water and adding cellulase to hydrolyze it to obtain a polygonatum hydrolyzate, filtering the polygonatum hydrolyzate, concentrating it, and freeze-drying it to obtain a polygonatum polysaccharide extract;

[0009] (4) adding water to the black plum and then inoculating it with lactic acid bacteria for fermentation, centrifuging to obtain the black plum fermentation liquid, and concentrating and freeze-drying the fermentation liquid to obtain the black plum fermentation extract;

[0010] (5) The four extracts of dendrobium, bitter almond, polygonatum and black plum are mixed in a weight ratio of 1:(1-3):(2-6):(0.05-0.5) to obtain a compound extract, and the composition is prepared into an oral product with a whitening effect.

[0011] A method for preparing extracts of dendrobium, bitter almond, polygonatum, and black plum, characterized in that the dendrobium is the dried stem of Dendrobium officinale Kimura et Migo, a plant of the orchid family; the bitter almond is the dried mature seed of Prunus armeniaca L.var.ansu Maxim., Prunus sibirica L., Prunus mandshurica (Maxim.) Koehne, or Prunus armeniaca L., a plant of the Rosaceae family; the polygonatum is the dried rhizome of Polygonatum odoratum (Mill.) Druce, a plant of the Liliaceae family; and the black plum is the dried nearly mature fruit of Prunus mume (Sieb.) Sieb. et Zucc., a plant of the Rosaceae family.

[0012] The preparation method of the dendrobium extract comprises the following steps: washing and cutting fresh dendrobium strips, freeze-drying the dried dendrobium strips to obtain a moisture content of ≤7%, crushing the dried dendrobium strips, and passing the dried dendrobium strips through a 60-mesh sieve to obtain dendrobium fine powder, wherein the dendrobium polysaccharide content is not less than 55%.

[0013] The bitter almond extract preparation method comprises the following steps: baking the bitter almonds at 60°C for 0.5-2h, adding 8-12 times the volume of 40-70% ethanol after crushing, adding 0.1-0.5% β-glucosidase, and reacting at 35-40°C for 2-5h, distilling to recover the ethanol, and drying to obtain the bitter almond extract. Preferably, the bitter almonds are baked at 60°C for 1h, crushed, adding 10 times the volume of 50% ethanol, adding 0.3% β-glucosidase, and reacting at 37°C for 4h.

[0014] The preparation method of the polysaccharide extract of polygonatum odoratum comprises the following steps: adding 5-10 times the volume of water after crushing polygonatum odoratum, adjusting the pH to 4.0-5.5, adding 0.2-0.5% cellulase (enzyme activity ≥10000U / g), performing enzymatic hydrolysis at 40-60°C for 1-3 hours, boiling to inactivate the enzyme, obtaining a polysaccharide hydrolyzate, filtering the polysaccharide hydrolyzate, concentrating, and freeze-drying to obtain the polysaccharide extract of polygonatum odoratum. Preferably, adding 8 times the volume of water after crushing polygonatum odoratum, adjusting the pH to 4.5, adding 0.5% cellulase (enzyme activity ≥10000U / g), and performing enzymatic hydrolysis at 50°C for 2 hours.

[0015] The method for preparing the black plum fermentation extract comprises the following steps: after removing the core of the black plum, adding 1-5 times the volume of water, inoculating 2-6% lactic acid bacteria (Lactobacillus plantarum), fermenting at 37°C for 24-72 hours, taking the supernatant after centrifugation to obtain the black plum fermentation liquid, and concentrating and freeze-drying the fermentation liquid to obtain the black plum fermentation extract; preferably, adding 2 times the volume of water, inoculating 5% lactic acid bacteria (Lactobacillus plantarum), and fermenting at 37°C for 48 hours.

[0016] The invention comprises the extract and composition prepared by the method, and pharmaceutically acceptable excipients, and the preparation is in the form of granules, tablets or oral liquid.

[0017] The compound extract is prepared into an oral liquid, and the volume proportion of the compound extract in the oral liquid is 3%-10%, and functional excipients such as oligofructose and vitamin C can be added.

[0018] Compared with the prior art, the method of the present invention has the following beneficial effects:

[0019] (1) This composition is a mixture of Dendrobium polysaccharide extract, Bitter Almond extract, Polygonatum polysaccharide extract, and Prunus mume fermented extract in a weight ratio of (1:1:2:0.1) to form a compound. The results of a zebrafish whitening experiment showed that the compound has a strong tyrosinase inhibitory effect. Further research found that the compound may regulate the expression of MITF and TYR genes by intervening in the occurrence of oxidative reactions, thereby reducing melanin synthesis. See the attached figure.

[0020] (2) The content of dendrobium polysaccharide extract in the composition is as high as 55%; the content of polygonatum polysaccharide is more than 80%; and the bitter almonds are treated with enzymes to remove toxic components, making oral administration safer.

[0021] (3) The preparation method of the present invention is mature and suitable for large-scale industrial production. The composition can be made into a functional food with whitening effect, which not only has whitening effect but also has good taste. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a visual diagram of the melanin area of ​​zebrafish embryos in each group.

[0023] Figure 2 It is a histogram of melanin area in zebrafish embryos of each group.

[0024] Figure 3 This is a comparison chart of the relative melanin content of zebrafish embryos in each group.

[0025] Figure 4 This is a comparison of tyrosinase activity levels in zebrafish embryos of each group.

[0026] Figure 5 This is a comparison of CAT activity levels in zebrafish embryos of each group.

[0027] Figure 6 This is a comparison of the expression levels of mitf, tyr, and tyrp2 genes in each group of zebrafish embryos. DETAILED DESCRIPTION

[0028] The present invention will be further described below with reference to the examples, but the present invention is not limited to the following examples.

[0029] Example 1

[0030] (1) Preparation of Dendrobium polysaccharide extract: Fresh Dendrobium strips were rinsed, cut, and freeze-dried under the following operating parameters: vacuum degree 0.01-0.05 mbar, cold well temperature -90--100°C, and the moisture content of the dried Dendrobium was about 6%. The extracted Dendrobium was crushed and passed through a 60-mesh sieve to obtain a fine powder having a polysaccharide content of not less than 55%.

[0031] (2) Preparation of bitter almond extract: Bitter almonds were roasted at 60°C for 1 hour, crushed, and then added with 8 times the volume of 60% ethanol and 0.3% β-glucosidase. The mixture was shaken at 37°C for 4 hours, and the ethanol was recovered by distillation and dried to obtain bitter almond extract.

[0032] (3) Preparation of Polygonatum odoratum polysaccharide extract: After crushing Polygonatum odoratum, add 8 times the volume of water, adjust the pH to 4.0, add 0.2% cellulase (enzyme activity ≥ 10000U / g), enzymatically hydrolyze at 50℃ for 2h, boil to inactivate the enzyme, and obtain Polygonatum odoratum enzymatic hydrolyzate. The Polygonatum odoratum enzymatic hydrolyzate is filtered, concentrated, and freeze-dried to obtain Polygonatum odoratum polysaccharide extract.

[0033] (4) Preparation of black plum fermentation extract: After removing the core of the black plum, add 3 times the volume of water, inoculate 5% lactic acid bacteria (Lactobacillus plantarum), ferment at 37°C for 24 hours, and collect the supernatant after centrifugation to obtain the black plum fermentation liquid. The fermentation liquid is concentrated and freeze-dried to obtain the black plum fermentation extract.

[0034] (5) The above-mentioned Dendrobium polysaccharide extract, bitter almond extract, Polygonatum odoratum polysaccharide extract, and Prunus mume fermented extract were mixed in a weight ratio of (1:1:2:0.1), diluted with water, and prepared into mixtures of different concentrations (292.5 μg / mL, 195 μg / mL, and 130 μg / mL), and the following zebrafish whitening experiment was performed.

[0035] Detection of melanin area in zebrafish embryos after treatment with mixed extracts:

[0036] 48 hpf embryos were selected and transferred to 12-well plates, with six embryos placed in each group. No drug was added to the blank group, while the remaining groups received 2 mL of pre-prepared high, medium, and low concentration drug solutions. After 24 hours of exposure, zebrafish embryos were anesthetized with 0.2% tricaine and fixed with 3% sodium carboxymethylcellulose. Embryos were uniformly adjusted to a left-side-up position and then transferred to a stereomicroscope for observation and recording. Quantitative analysis was performed using ImageJ software.

[0037] Test results are shown in Figure 1 and Figure 2The results showed that in the blank group, the embryonic melanin was darker in color and distributed over a larger area on the head and abdomen. As the drug concentration increased, the melanin distribution area gradually decreased. The blank control group had the highest melanin area, while the melanin area in the high and medium concentration treatment groups was significantly reduced (P<0.05), demonstrating a strong inhibitory effect. The melanin area in the low concentration treatment group had a slight inhibitory effect on melanin production compared to the blank group. The inhibitory effect of the medium concentration group was slightly weaker than that of the high concentration group; at higher concentrations, melanin production was significantly reduced.

[0038] Table 1 Melanin area of ​​zebrafish embryos in each group

[0039]

[0040] Note: Compared with the blank control group, a P<0.05; compared with the low-dose group, b P<0.01, c P<0.001; compared with the arbutin group, d P<0.05, e P<0.01, f P<0.001

[0041] Determination of tyrosinase activity and relative melanin content in zebrafish embryos after treatment with mixed extracts:

[0042] 48hpf embryos were selected and transferred to a 6-well plate, with three replicates per group and 40 embryos placed in each well. No drugs were added to the blank group, and high, medium, and low concentrations of drug solutions were added to the remaining groups. When the embryos developed to 72hpf, the drug solution was aspirated and washed three times with embryo culture water. Subsequently, the embryos were transferred to a glass homogenizer by group, 150.0μL of sodium deoxycholate solution (5.0mg / mL) was added, and homogenized in an ice bath. The samples were then centrifuged at 10,000rpm for 5 minutes at 4°C, and the supernatant and precipitate were collected after centrifugation.

[0043] Add 300 μL of sodium hydroxide solution (1.0 mol / L) to the precipitate and sonicate for 1 hour until the precipitate is completely dissolved. Take 100.0 μL of sample solution and add it to a 96-well plate. Take another 100.0 μL of sodium hydroxide solution as a zero-adjustment control group. Place the sample in a microplate reader and measure the absorbance at a wavelength of 405 nm. Melanin content inhibition rate (%) = [1-(OD sample group-OD zero-adjustment group) / (OD blank control group-OD zero-adjustment group)] × 100%;

[0044] The protein concentration in the supernatant was determined using a BCA kit. 15 μL of sample was taken from each group, diluted 20-fold, and then 300 μL of levodopa solution (5.0 mmol / L) was added. 200 μL of sample solution was added to a 96-well plate, and another 200 μL of lysate was mixed with levodopa solution to serve as the zero-adjustment control group. All samples were incubated in a 37°C constant temperature incubator for 1 hour, and the absorbance was measured at a wavelength of 475 nm using a microplate reader. Tyrosinase activity inhibition rate (%) = [1-(OD sample group-OD zero-adjustment group) / (OD blank control group-OD zero-adjustment group)] × 100%.

[0045] The analysis results are shown in Figure 3 and Figure 4 The melanin content of the positive control group (arbutin group) was reduced by about 50% compared with the blank control group. The inhibition rates of the high, medium and low concentration treatment groups decreased in sequence. The results showed that the mixed extract reduced the melanin content of zebrafish embryos. By calculating the inhibition rate of tyrosinase activity in the mixed extract treatment group, it was found that the inhibition rate gradually increased with increasing concentration, and the inhibition rate of the high concentration group reached about 50%. Tyrosinase activity directly determines the efficiency of melanin synthesis. The experiment proved that by quantifying the inhibitory effect of the mixed extract on tyrosinase activity, the potential role of the mixed extract in inhibiting melanin production was further demonstrated.

[0046] Table 2 Inhibition rate of TYR activity and melanin content in zebrafish embryos of each group (%)

[0047]

[0048] Note: Compared with the high-dose group, a P<0.05, b P<0.01, c P<0.001; compared with the arbutin group, d P<0.01, e P<0.001Determination of catalase activity in zebrafish embryos after treatment with mixed extracts:

[0049] 48hpf embryos were selected and transferred to a 6-well plate, with 3 replicates per group and 40 embryos placed in each well. No drug was added to the blank group, and 6 mL of drug solution of different concentrations was added to the other groups. At 72hpf, all the drug solution was aspirated and washed 3 times with embryo culture medium. The larvae were then transferred to a 1.5 mL centrifuge tube, and physiological saline was added at a ratio of 1:10 of larval weight (g) to 0.9% NaCl solution (mL). The tube was homogenized in an ice bath to obtain 10% tissue fluid of the larvae. The tube was centrifuged at 4°C and 10,000 r / min for 7 minutes, and the supernatant was collected. The protein concentration of the sample was determined using a BCA kit and adjusted uniformly. The color working solution was prepared according to the instructions of the CAT detection kit, and a standard curve of hydrogen peroxide content and absorbance was drawn. Finally, the catalase activity in the sample was detected and calculated.

[0050] The analysis results are shown in Figure 5 The results showed that the mixed extract can significantly enhance CAT activity. The blank control group had the lowest CAT activity, while the low-concentration mixed extract-treated group had significantly higher CAT activity than the blank group (P<0.01). The high-concentration mixed extract group had the highest CAT activity, while the medium-concentration group had slightly lower CAT activity than the high-concentration group, but still significantly higher than the arbutin group (P<0.05). The experimental results showed that the mixed extract can achieve an antioxidant effect by enhancing CAT activity, and it is speculated that the mixed extract may indirectly inhibit the melanin production process by enhancing CAT activity. The experiment also showed that the difference in CAT activity between the mixed extract groups with different concentrations was small, and the enhancing effect of the mixed extract on CAT activity may exist within a certain concentration range and reach a saturation effect.

[0051] Table 3 CAT activity levels of zebrafish embryos in each group

[0052]

[0053]

[0054] Note: Compared with the blank control group, a P<0.01, b P<0.001; compared with the arbutin group, c P<0.05, d P<0.01; compared with the high-dose group, e P<0.05

[0055] Determination of mitf, tyr, and tyrp2 gene expression levels in zebrafish embryos after treatment with the mixed extract:

[0056] Zebrafish embryos from each group were collected and total RNA was extracted using the TRIzol method. Subsequently, the extracted RNA was reverse transcribed into cDNA using a reverse transcription kit and subjected to real-time fluorescence quantitative PCR. The reaction conditions were pre-denaturation at 95°C for 1 min, 95°C for 20 s, and 60°C for 1 min for 40 cycles. Gapdh was used as an internal reference, and primer sequences were designed according to references. 2 -△△Ct The target gene expression levels were analyzed.

[0057] The analysis results are shown in Figure 6 Results showed that MITF and TYR mRNA expression levels were significantly inhibited by high, medium, and low drug concentrations (P<0.01). The mixed extract treatment group exhibited a weak inhibitory effect on TYRP2 gene expression, but there was no significant difference in expression compared to the blank control group. Overall, the high-concentration mixed extract was more effective in inhibiting MITF and TYR mRNA expression than the lower concentration. This suggests that the mixed extract may inhibit the activity of melanin-related enzymes and melanin production by interfering with MITF-related pathways in zebrafish embryos.

[0058] Table 4 MITF, TYR and TYRP2 mRNA expression levels in zebrafish embryos of each group

[0059]

[0060] Note: Compared with the blank control group, a P<0.001, b P<0.01, c P<0.05

[0061] Example 2

[0062] (1) Preparation of Dendrobium polysaccharide extract: Fresh Dendrobium strips were rinsed, cut, and freeze-dried under vacuum conditions of 0.01 to 5 mbar and a cold well temperature of -90 to -100°C. The dried Dendrobium strips had a moisture content of approximately 6%. The strips were crushed and passed through a 60-mesh sieve to obtain fine Dendrobium powder containing no less than 55% polysaccharide.

[0063] (2) Preparation of bitter almond extract: Bitter almonds were roasted at 60°C for 1 hour, crushed, and then 8 times the volume of 50% ethanol was added. 0.3% β-glucosidase was added and the mixture was shaken at 37°C for 4 hours. The ethanol was recovered by distillation and dried to obtain bitter almond extract.

[0064] (3) Preparation of Polygonatum odoratum polysaccharide extract: After crushing Polygonatum odoratum, add 8 times the volume of water, adjust the pH to 4.5, add 0.5% cellulase (enzyme activity ≥ 10000U / g), enzymatically hydrolyze at 50℃ for 2h, boil to inactivate the enzyme, and obtain Polygonatum odoratum enzymatic hydrolyzate. The Polygonatum odoratum enzymatic hydrolyzate is filtered, concentrated, and freeze-dried to obtain Polygonatum odoratum polysaccharide extract.

[0065] (4) Preparation of black plum fermentation extract: After removing the core of the black plum, add 3 times the volume of water, inoculate 4% lactic acid bacteria (Lactobacillus plantarum), ferment at 37°C for 24 hours, and collect the supernatant after centrifugation to obtain the black plum fermentation liquid. The fermentation liquid is concentrated and freeze-dried to obtain the black plum fermentation extract.

[0066] (5) The above-mentioned dendrobium polysaccharide extract, bitter almond extract, polygonatum polysaccharide extract, and black plum fermentation extract are mixed in a weight ratio of (1:1:2:0.2).

[0067] (6) Granulate the mixed extract of (5), soluble starch and sucrose in a weight ratio of (1:2:1), dry, and pack into 2 g / bag.

[0068] Example 3

[0069] (1) Preparation of Dendrobium polysaccharide extract: Fresh Dendrobium strips were rinsed, cut, and freeze-dried under the following operating parameters: vacuum degree 0.01-1 mbar, cold well temperature -80--100°C, and the moisture content of the dried Dendrobium was about 7%. The extracted Dendrobium strips were crushed and passed through a 60-mesh sieve to obtain a fine powder having a polysaccharide content of not less than 55%.

[0070] (2) Preparation of bitter almond extract: Bitter almonds were roasted at 60°C for 1 hour, crushed, and added with 10 times the volume of 60% ethanol, 0.5% β-glucosidase, and reacted at 37°C for 4 hours. The ethanol was recovered by distillation and dried to obtain bitter almond extract.

[0071] (3) Preparation of Polygonatum odoratum polysaccharide extract: After crushing Polygonatum odoratum, add 8 times the volume of water, adjust the pH to 4.0, add 0.4% cellulase (enzyme activity ≥ 10000U / g), enzymatically hydrolyze at 50℃ for 2h, boil to inactivate the enzyme, and obtain Polygonatum odoratum enzymatic hydrolyzate. The Polygonatum odoratum enzymatic hydrolyzate is filtered, concentrated, and freeze-dried to obtain Polygonatum odoratum polysaccharide extract.

[0072] (4) Preparation of black plum fermentation extract: After removing the core of the black plum, add 3 times the volume of water, inoculate 3% lactic acid bacteria (Lactobacillus plantarum), ferment at 37°C for 48 hours, and collect the supernatant after centrifugation to obtain the black plum fermentation liquid. The fermentation liquid is concentrated and freeze-dried to obtain the black plum fermentation extract.

[0073] (5) The above-mentioned dendrobium polysaccharide extract, bitter almond extract, polygonatum polysaccharide extract, and black plum fermentation extract are mixed in a weight ratio of (1:2:3:0.1).

[0074] (6) Mix the mixed extract of (5) with oligofructose and vitamin C in a weight ratio of (1:2:0.05), dilute with water to 100 times the amount, and package into 50 ml / bottles.

Claims

1. A method for preparing a composition of a traditional Chinese medicine whitening extract, characterized in that: The following steps are involved: (1) Slicing fresh dendrobium strips, freeze-drying, crushing, and sieving to obtain a dendrobium polysaccharide extract; (2) roasting the bitter almonds, adding ethanol, and adding β-glucosidase to convert the bitter almonds, distilling to recover the ethanol, and drying to obtain the bitter almond extract; (3) crushing the polygonatum, adding water and adding cellulase to hydrolyze it to obtain a polygonatum hydrolyzate, filtering the polygonatum hydrolyzate, concentrating it, and freeze-drying it to obtain a polygonatum polysaccharide extract; (4) adding water to the black plum and then inoculating it with lactic acid bacteria for fermentation, centrifuging to obtain the black plum fermentation liquid, and concentrating and freeze-drying the fermentation liquid to obtain the black plum fermentation extract; (5) The four extracts of dendrobium, bitter almond, polygonatum and black plum are mixed in a weight ratio of 1:(1-3):(2-6):(0.05-0.5) to obtain a compound extract.

2. The method according to claim 1, characterized in that The dendrobium is the dried stem of Dendrobium officinale Kimura et Migo of the Orchidaceae family; the bitter almond is the dried mature seed of Prunus armeniaca L. var. ansu Maxim., Prunus sibirica L., Prunus mandshurica (Maxim.) Koehne or Prunus armeniaca L. of the Rosaceae family; The polygonatum odoratum is the dried rhizome of Polygonatum odoratum (Mill.) Druce of the Liliaceae family; The black plum is the dried nearly mature fruit of the Rosaceae plant Prunus mume (Sieb.) Sieb. et Zucc.

3. The method according to claim 1, characterized in that Step (1) The fresh dendrobium strips are washed, cut into sections, and freeze-dried. The dried dendrobium strips have a moisture content of ≤7%, are crushed, and passed through a 60-mesh sieve to obtain dendrobium fine powder, wherein the dendrobium polysaccharide content is not less than 55%.

4. The method according to claim 1, characterized in that Step (2) Bitter almonds are roasted at 60°C for 0.5-2h, crushed, and then 8-12 times the volume of 40-70% ethanol and 0.1-0.5% β-glucosidase are added. The mixture is shaken at 35-40°C for 2-5 hours, ethanol is recovered by distillation, and dried to obtain a bitter almond extract. Preferably, the bitter almonds are roasted at 60°C for 1 hour, crushed, and then 10 times the volume of 50% ethanol is added, 0.3% β-glucosidase is added, and the mixture is shaken at 37°C for 4 hours.

5. The method according to claim 1, characterized in that Step (3) After the Polygonatum odoratum is crushed, 5-10 times the volume of water is added, the pH is adjusted to 4.0-5.5, 0.2-0.5% cellulase (enzyme activity ≥ 10000U / g) is added, and enzymatic hydrolysis is carried out at 40-60°C for 1-3 hours, and the enzyme is boiled to inactivate the enzyme to obtain a Polygonatum odoratum enzymatic hydrolyzate, which is filtered, concentrated, and freeze-dried to obtain a Polygonatum odoratum polysaccharide extract. Preferably, after the Polygonatum odoratum is crushed, 8 times the volume of water is added, the pH is adjusted to 4.5, 0.5% cellulase (enzyme activity ≥ 10000U / g) is added, and enzymatic hydrolysis is carried out at 50°C for 2 hours.

6. The method according to claim 1, characterized in that Step (4) After removing the core of the black plum, add 1-5 times the volume of water, inoculate 2-6% lactic acid bacteria (Lactobacillus plantarum), ferment at 37°C for 24-72h, centrifuge and take the supernatant. Obtaining a fermented plum liquor, concentrating the fermented liquor, and freeze-drying the fermented plum extract; preferably, adding 2 times the volume of water, inoculating 5% lactic acid bacteria (Lactobacillus plantarum), and fermenting at 37° C. for 48 hours.

7. A composition prepared according to the method of any one of claims 1 to 6.

8. Use of the composition prepared by the method according to any one of claims 1 to 6 for preparing an oral whitening skin care product.

9. A whitening oral preparation, characterized in that: A composition prepared by the method according to any one of claims 1 to 6, further comprising a pharmaceutically acceptable excipient, wherein the preparation is in the form of granules, tablets or oral solution.

10. The oral whitening preparation according to claim 9, characterized in that: The extract is prepared into an oral liquid, with the volume proportion of the extract in the oral liquid being 3%-10%, and functional auxiliary materials such as oligofructose and vitamin C can be added.

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