Human mesenchymal stem cell culture and separation device and method thereof

By designing a cell culture separation device with a rotating cylinder, stirring blades and cleaning scrapers, the problems of low extraction efficiency and low yield of highly active mesenchymal stem cells are solved, and efficient and lossless cell collection is achieved.

CN120699770AInactive Publication Date: 2025-09-26BEIJING ZHONGKE JIANLAN GRP CO LTD
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Patent Information

Application Number
CN202510898085.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-01
Publication Date
2025-09-26
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

In the existing technology, during the culture process of mesenchymal stem cells, highly active cells are difficult to extract efficiently, resulting in low extraction efficiency and low yield, and cell adhesion and waste are easily caused when the culture medium is replaced.

Method used

A human mesenchymal stem cell culture and separation device was designed, which includes a rotating cylinder, stirring blades, a cleaning element, and a scraping element. The device uses a motor to drive stirring and centrifugal separation of cells, and uses an electric push rod and a scraper to clean the cells. Combined with a hydraulic cylinder lifting device, efficient collection is achieved.

Benefits of technology

The extraction efficiency and yield of highly active mesenchymal stem cells are improved, cell adhesion and waste are reduced, and the operation process is simplified.

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Abstract

The invention discloses a human mesenchymal stem cell culture and separation device and a method thereof.The human mesenchymal stem cell culture and separation device comprises a culture bottle and a base arranged at the bottom of the culture bottle, a rotating cylinder is rotationally arranged in the culture bottle, stirring blades are fixedly arranged on the surface of the rotating cylinder, and a cleaning piece is arranged on the surface of the rotating cylinder; a collecting box is movably arranged in the base, and a scraping piece is arranged in the collecting box. The cleaning part comprises a cleaning plate which is arranged on the surface of the rotating cylinder in a sliding manner. According to the human mesenchymal stem cell culture and separation device and method, through the arrangement of the cleaning part and the scraping part, high-activity mesenchymal stem cells adhering to the inner wall of the culture bottle and the surfaces of the stirring blades and the rotating cylinder can be scraped into the collecting box, unified collection by personnel is facilitated, the personnel do not need to stretch a tool into the bottle body for extraction, and the working efficiency is improved. The working intensity is reduced, and meanwhile, the extraction efficiency and the yield of the high-activity mesenchymal stem cells are also improved.
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Citation Information

Patent Citations

  • Mesenchymal stem cell culture and separation device

    CN212476783U