Plasma nano amino acid foliar fertilizer bacterial fertilizer and quality control method thereof

Abstract

The invention relates to the technical field of fertilizers, and discloses a plasma nano amino acid foliar bacterial fertilizer and a quality control method thereof, the plasma nano amino acid foliar bacterial fertilizer comprises 50-150mg / L of a plasma nano carrier, 2 * 10 < 8 >-1 * 10 < 9 > cfu / mL of a compound microbial agent, 15-30 parts of amino acid, 5-60mg / L of medium trace elements and 10-20 parts of a function enhancer; wherein the plasma nano carrier is a silver nano particle coated with silicon dioxide, or a TiO2 hollow nanosphere, or magnetic Fe3O4; the compound microbial agent is formed by compounding bacillus subtilis and phosphate solubilizing bacteria according to the ratio of 2: 1; the amino acid is yeast source amino acid, or tyrosine derivative, or gamma-aminobutyric acid; the medium trace elements are Fe, Zn and Mn, or Ca and Mg, or Se; the functional synergist is humic acid, trehalose or ascorbic acid, and ensures that the nutrient release behavior is dynamically matched with the crop demand.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and in particular to a plasma nano-amino acid foliar fertilizer and a bacterial fertilizer and a quality control method thereof. Background Art

[0002] In the field of agricultural production, especially in the areas of crop nutrition supply and disease control, the synergistic effects, environmental friendliness, and functional synergy of foliar fertilizers and microbial fertilizers have become a research hotspot. However, existing technologies generally suffer from key issues such as uncontrolled nutrient release, poor microbe-carrier synergy, and a disconnect between disease control and nutrient supply, which seriously hinder the green transformation and sustainable development of agriculture.

[0003] Chinese patent application CN103992191A discloses a composite biofertilizer composed of 30-40 parts of edible fungus waste extract, 30-40 parts of soybean meal extract, 5-10 parts of a mixed bacterial solution, 2-3 parts of potassium humate, and 1-2 parts of aminoethyl hexanoate. The invention also discloses a method for preparing the biofertilizer. While the biofertilizer has advantages such as increased yield, disease resistance, and environmental friendliness, it does not address the issue of dynamically matching nutrient release rates with crop demand. Summary of the Invention

[0004] To this end, the present invention provides a plasma nano-amino acid foliar fertilizer to overcome the problems of single release behavior and poor environmental adaptability of traditional foliar fertilizers.

[0005] In one aspect, the present invention provides a plasma nano-amino acid foliar fertilizer, comprising:

[0006] Plasma nanocarrier 50-150mg / L, composite microbial agent 2×10 8 -1×10 9 cfu / mL, amino acids 15-30, trace elements 5-60mg / L, and functional enhancers 10-20 parts;

[0007] Wherein, the plasma nanocarrier is silica-coated silver nanoparticles, or TiO2 hollow nanospheres, or magnetic Fe3O4;

[0008] The composite microbial agent is a 2:1 mixture of Bacillus subtilis and phosphate-solubilizing bacteria;

[0009] The amino acid is a yeast-derived amino acid, a tyrosine derivative, or γ-aminobutyric acid;

[0010] The trace elements are Fe, Zn and Mn, or Ca and Mg, or Se;

[0011] The functional enhancer is humic acid, trehalose, or ascorbic acid.

[0012] Furthermore, the plasma nanocarriers, amino acids, trace elements and functional enhancers are selected according to different components in the seedling stage, flowering and fruiting stage and late fruiting stage.

[0013] Furthermore, during the flowering and fruiting period, the plasma nanocarriers, amino acids, trace elements and functional enhancers are selected from the following components:

[0014] The silica-coated silver nanoparticles 50-80 mg / L, the composite microbial agent 10 9 cfu / mL, 15 g / L of yeast-derived amino acids, and 10 parts of humic acid;

[0015] The trace elements selected are Fe, Zn and Mn, each at 20 mg / L.

[0016] During the flowering and fruiting period, the plasma nanocarriers, amino acids, trace elements and functional enhancers are selected from the following components:

[0017] The TiO2 hollow nanospheres 80-120 mg / L, composite microbial agent 5×10 8 cfu / mL, tyrosine derivative 20 g / L and trehalose 15 parts;

[0018] The trace elements selected are Ca and Mg, 30 mg / L each.

[0019] The components selected from the plasma nanocarrier, amino acid, trace elements and functional enhancer are:

[0020] The magnetic Fe3O4100-150mg / L, composite microbial agent 2×10 8 cfu / mL, 30 g / L of γ-aminobutyric acid, and 20 parts of ascorbic acid;

[0021] The trace element is Se 5 mg / L.

[0022] In another aspect, the present invention provides a quality control method for plasma nano-amino acid foliar fertilizer, comprising the following steps:

[0023] By simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency.

[0024] Adjust the carrier modification process and functional component loading parameters based on the surface functional group characteristics of the nanocarrier and the metabolic activity of the microorganisms;

[0025] Adopt online monitoring and feedback adjustment system to ensure the batch stability of nanocarrier structure, microbial activity and release curve in batch production;

[0026] Evaluate the nutrient retention and ecological safety of products under adverse conditions through salt stress, drought simulation and leaching experiments;

[0027] Integrate near-infrared spectroscopy (NIR), flow cytometers, and manufacturing execution systems (MES) to achieve full quality traceability and dynamic control from raw materials to finished products.

[0028] The crop microenvironment includes pH, temperature and humidity.

[0029] Furthermore, by simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency, including:

[0030] pH gradient test, set 5 gradient intervals of 1.0 in the pH range of 3.0-9.0, and measure the nutrient release rate over 24 hours;

[0031] Temperature gradient test: set 5 gradients with intervals of 5°C in the range of 20°C-40°C to measure the nutrient release rate;

[0032] Humidity simulation: Control the humidity between 40% and 90% RH with saturated salt solution to verify the moisture absorption and release balance of the carrier;

[0033] The release curve was fitted using the Korsmeyer-Peppas equation, the release index was calculated, and the release mechanism was determined to be:

[0034] Diffusion control n<0.45, skeleton dissolution 0.45 <n<0.89。

[0035] The release efficiency benchmark is set as a release rate of ≥60% in the pH range of 5.5-6.8 and a release rate of ≥80% over 24 hours at 30°C.

[0036] Furthermore, based on the surface functional group characteristics of the nanocarrier and the metabolic activity of the microorganisms, the carrier modification process and functional component loading parameters are adjusted, including:

[0037] The surface -NH2 functional group content was determined by X-ray photoelectron spectroscopy (XPS), with a target value of 1.2-1.5 mmol / gSiO2;

[0038] Zeta potential was verified using electrophoretic light scattering (ELS), with a target value >-30 mV to ensure pH response sensitivity.

[0039] Immobilized bacterial agent loading ≥1×10 10 CFU / g carrier, and the viable bacteria rate ≥90%;

[0040] Functional gene expression verification: qPCR was used to detect the expression of the phoD gene of phosphate-solubilizing bacteria, with a target value of ΔCt≤2.0;

[0041] The freeze-drying protective agent formula is 20% trehalose and 10% glycerol.

[0042] Furthermore, an online monitoring and feedback control system is used to ensure the batch stability of nanocarrier structure, microbial activity and release profile during mass production, including:

[0043] Near infrared spectroscopy (NIR) was used to monitor the functional groups of nanocarriers in real time, and the -NH2 peak intensity fluctuation was set to ≤5%.

[0044] Flow cytometry was used to dynamically detect microbial activity, with a viable bacterial rate set at ≥95% and sampling every 30 minutes;

[0045] The MES system is linked to the pH / temperature sensor to automatically calibrate the reactor stirring speed and set a ±5% fluctuation warning;

[0046] If the release curve deviates from the preset model by more than 10%, a shutdown alarm is triggered and the abnormal batch is locked.

[0047] Furthermore, through salt stress, drought simulation and leaching experiments, the nutrient retention rate and ecological safety of the product under adverse conditions are evaluated, including:

[0048] Salt stress test, NaCl concentration gradient 0-300mM, determination of chlorophyll fluorescence parameter Fv / Fm recovery rate ≥ 90%;

[0049] Drought simulation, PEG 6000 concentration gradient 0-20%, verifying the sustained nutrient release rate of ≤15% over 7 days;

[0050] Leaching experiment, Ag + Mobility detection ICP-MS method, set the target value <0.1mg / L;

[0051] The effects on soil microbial communities were analyzed by high-throughput sequencing. The Shannon index of soil bacterial diversity was set to decrease by ≤10% after application.

[0052] Compared with the existing technology, the beneficial effect of the present invention lies in that the present invention verifies the response sensitivity of the nanocarrier to environmental parameters by simulating the release kinetics test of the crop microenvironment (pH / temperature / humidity), ensures the dynamic matching of nutrient release behavior with crop needs, and solves the problem of single release behavior and poor environmental adaptability of traditional foliar fertilizers.

[0053] Furthermore, by quantitatively regulating the proportion of functional groups on the surface of nanocarriers and the metabolic activity of microorganisms, the degree of carrier modification and the loading efficiency of functional components can be optimized, breaking through the limitations of conventional foliar fertilizers that rely on empirical formulas.

[0054] Furthermore, an online monitoring and feedback adjustment system is used to eliminate fluctuations in nanocarrier structure, microbial activity and release curves during batch production, ensure batch stability of product performance, and overcome the shortcomings of lagging traditional process quality control methods.

[0055] Furthermore, through adversity simulation experiments (salt stress, drought, leaching), the product's nutrient retention rate and ecological safety under complex field conditions are verified, solving the pain points of conventional foliar fertilizers being easily affected by environmental interference and having a high risk of heavy metal residues.

[0056] Furthermore, by integrating near-infrared spectroscopy (NIR), flow cytometers and manufacturing execution systems (MES), we can achieve full quality traceability and dynamic regulation from raw materials to finished products, replacing the traditional manual sampling mode and improving production efficiency and quality control accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 This is a flow chart of the steps of plasma nano-amino acid foliar fertilizer and bacterial fertilizer and its quality control method according to an embodiment of the present invention.

[0058] Figure 2 This is a flow chart of the steps for optimizing environmental response parameters according to an embodiment of the present invention.

[0059] Figure 3 This is a flowchart of the steps of release behavior consistency control according to an embodiment of the present invention.

[0060] Figure 4 A roadmap for verifying the environmental adaptability of an embodiment of the present invention. DETAILED DESCRIPTION

[0061] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are merely used to explain the present invention and are not intended to limit the present invention.

[0062] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0063] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside", and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0064] In the invention description, verification delay refers to the time it takes for engineering technicians to detect conflicts when carrying out construction according to the BIM model; adjustment records refer to the number of times engineering technicians modify BIM model data or generate conflict detection when carrying out construction according to the BIM model.

[0065] Furthermore, it should be noted that, in the description of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0066] In one aspect, the present invention provides a plasma nano-amino acid foliar fertilizer, comprising:

[0067] Plasma nanocarrier 50-150mg / L, composite microbial agent 2×10 8 -1×10 9 cfu / mL, amino acids 15-30, trace elements 5-60mg / L, and functional enhancers 10-20 parts;

[0068] Wherein, the plasma nanocarrier is silica-coated silver nanoparticles, or TiO2 hollow nanospheres, or magnetic Fe3O4;

[0069] The composite microbial agent is a 2:1 mixture of Bacillus subtilis and phosphate-solubilizing bacteria;

[0070] The amino acid is a yeast-derived amino acid, a tyrosine derivative, or γ-aminobutyric acid;

[0071] The trace elements are Fe, Zn and Mn, or Ca and Mg, or Se;

[0072] The functional enhancer is humic acid, trehalose, or ascorbic acid.

[0073] Specifically, different components of plasma nanocarriers, amino acids, trace elements and functional enhancers are selected according to the seedling stage, flowering and fruiting stage and late fruiting stage.

[0074] Specifically, during the flowering and fruiting period, the components selected for plasma nanocarriers, amino acids, trace elements and functional enhancers are:

[0075] Silica coated silver nanoparticles 50-80 mg / L, composite microbial agent 10 9 cfu / mL, 15 g / L of yeast-derived amino acids, and 10 parts of humic acid;

[0076] The trace elements used were Fe, Zn and Mn at 20 mg / L each.

[0077] During the flowering and fruiting period, the components selected for plasma nanocarriers, amino acids, trace elements and functional enhancers are:

[0078] TiO2 hollow nanospheres 80-120 mg / L, composite microbial agent 5×10 8 cfu / mL, tyrosine derivative 20 g / L and trehalose 15 parts;

[0079] The trace elements used were Ca and Mg at 30 mg / L each.

[0080] The components selected for plasma nanocarriers, amino acids, trace elements and functional enhancers are:

[0081] Magnetic Fe3O4100-150mg / L, composite microbial agent 2×10 8 cfu / mL, 30 g / L of γ-aminobutyric acid, and 20 parts of ascorbic acid;

[0082] Se 5mg / L was selected as the trace element.

[0083] See also Figure 1 As shown, Figure 1 This is a flow chart of the steps of plasma nano-amino acid foliar fertilizer and bacterial fertilizer and its quality control method according to an embodiment of the present invention.

[0084] In another aspect, the present invention provides a quality control method for plasma nano-amino acid foliar fertilizer, comprising the following steps:

[0085] By simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency.

[0086] Adjust the carrier modification process and functional component loading parameters based on the surface functional group characteristics of the nanocarrier and the metabolic activity of the microorganisms;

[0087] Adopt online monitoring and feedback adjustment system to ensure the batch stability of nanocarrier structure, microbial activity and release curve in batch production;

[0088] Evaluate the nutrient retention and ecological safety of products under adverse conditions through salt stress, drought simulation and leaching experiments;

[0089] Integrate near-infrared spectroscopy (NIR), flow cytometry, and manufacturing execution system (MES) to achieve full quality traceability and dynamic control from raw materials to finished products;

[0090] The crop microenvironment includes pH, temperature and humidity.

[0091] Specifically, by simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency, including:

[0092] pH gradient test, set 5 gradient intervals of 1.0 in the pH range of 3.0-9.0, and measure the nutrient release rate over 24 hours;

[0093] Temperature gradient test: set 5 gradients with intervals of 5°C in the range of 20°C-40°C to measure the nutrient release rate;

[0094] Humidity simulation: Control the humidity between 40% and 90% RH with saturated salt solution to verify the moisture absorption and release balance of the carrier;

[0095] The release curve was fitted using the Korsmeyer-Peppas equation, the release index was calculated, and the release mechanism was determined to be:

[0096] Diffusion control n<0.45, skeleton dissolution 0.45 <n<0.89。

[0097] The release efficiency benchmark is set as a release rate of ≥60% in the pH range of 5.5-6.8 and a release rate of ≥80% over 24 hours at 30°C.

[0098] See also Figure 2 , Figure 2 This is a flow chart of the steps for optimizing environmental response parameters according to an embodiment of the present invention.

[0099] Specifically, based on the surface functional group characteristics of the nanocarrier and the metabolic activity of the microorganisms, the carrier modification process and functional component loading parameters are adjusted, including:

[0100] The surface -NH2 functional group content was determined by X-ray photoelectron spectroscopy (XPS), with a target value of 1.2-1.5 mmol / gSiO2;

[0101] Zeta potential was verified using electrophoretic light scattering (ELS), with a target value >-30 mV to ensure pH response sensitivity.

[0102] Immobilized bacterial agent loading ≥1×10 10 CFU / g carrier, and the viable bacteria rate ≥90%;

[0103] Functional gene expression verification: qPCR was used to detect the expression of the phoD gene of phosphate-solubilizing bacteria, with a target value of ΔCt≤2.0;

[0104] The freeze-drying protective agent formula is 20% trehalose and 10% glycerol.

[0105] See also Figure 3, Figure 3 This is a flowchart of the steps of release behavior consistency control according to an embodiment of the present invention.

[0106] Specifically, an online monitoring and feedback control system is used to ensure batch stability of nanocarrier structure, microbial activity, and release profile during mass production, including:

[0107] Near infrared spectroscopy (NIR) was used to monitor the functional groups of nanocarriers in real time, and the -NH2 peak intensity fluctuation was set to ≤5%.

[0108] Flow cytometry was used to dynamically detect microbial activity, with a viable bacterial rate set at ≥95% and sampling every 30 minutes;

[0109] The MES system is linked to the pH / temperature sensor to automatically calibrate the reactor stirring speed and set a ±5% fluctuation warning;

[0110] If the release curve deviates from the preset model by more than 10%, a shutdown alarm is triggered and the abnormal batch is locked.

[0111] See also Figure 4 , Figure 4 A roadmap for verifying the environmental adaptability of an embodiment of the present invention.

[0112] Specifically, through salt stress, drought simulation and leaching experiments, we evaluate the nutrient retention and ecological safety of products under adverse conditions, including:

[0113] Salt stress test, NaCl concentration gradient 0-300mM, determination of chlorophyll fluorescence parameter Fv / Fm recovery rate ≥ 90%;

[0114] Drought simulation, PEG 6000 concentration gradient 0-20%, verifying the sustained nutrient release rate of ≤15% over 7 days;

[0115] Leaching experiment, Ag + Mobility detection ICP-MS method, set the target value <0.1mg / L;

[0116] The effects on soil microbial communities were analyzed by high-throughput sequencing. The Shannon index of soil bacterial diversity was set to decrease by ≤10% after application.

[0117] Thus far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art may make equivalent changes or substitutions to the relevant technical features, and the technical solutions after such changes or substitutions will fall within the scope of protection of the present invention.

[0118] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A plasma nano amino acid foliar fertilizer, characterized in that: include: Plasma nanocarrier 50-150mg / L, composite microbial agent 2×10 8 -1×10 9 cfu / mL, amino acids 15-30, trace elements 5-60mg / L, and functional enhancers 10-20 parts; Wherein, the plasma nanocarrier is silica-coated silver nanoparticles, or TiO2 hollow nanospheres, or magnetic Fe3O4; The composite microbial agent is a 2:1 mixture of Bacillus subtilis and phosphate-solubilizing bacteria; The amino acid is a yeast-derived amino acid, a tyrosine derivative, or γ-aminobutyric acid; The trace elements are Fe, Zn and Mn, or Ca and Mg, or Se; The functional enhancer is humic acid, trehalose, or ascorbic acid.

2. The plasma nano amino acid foliar fertilizer according to claim 1, characterized in that The plasma nanocarriers, amino acids, trace elements and functional enhancers are selected according to different components in the seedling stage, flowering and fruiting stage and late fruiting stage.

3. The plasma nano amino acid foliar fertilizer according to claim 2, characterized in that During the flowering and fruiting period, the plasma nanocarriers, amino acids, trace elements and functional enhancers are selected from the following components: The silica-coated silver nanoparticles 50-80 mg / L, the composite microbial agent 10 9 cfu / mL, 15 g / L of yeast-derived amino acids, and 10 parts of humic acid; The trace elements selected are Fe, Zn and Mn, each at 20 mg / L.

4. The plasma nano amino acid foliar fertilizer according to claim 2, characterized in that During the flowering and fruiting period, the plasma nanocarriers, amino acids, trace elements and functional enhancers are selected from the following components: The TiO2 hollow nanospheres 80-120 mg / L, composite microbial agent 5×10 8 cfu / mL, tyrosine derivative 20 g / L and trehalose 15 parts; The trace elements selected are Ca and Mg, 30 mg / L each.

5. The plasma nano amino acid foliar fertilizer according to claim 2, characterized in that In the later stage of the results, the components selected for the plasma nanocarriers, amino acids, trace elements and functional enhancers are: The magnetic Fe3O4100-150mg / L, composite microbial agent 2×10 8 cfu / mL, 30 g / L of γ-aminobutyric acid, and 20 parts of ascorbic acid; The trace element is Se 5 mg / L.

6. The quality control method of the plasma nano-amino acid foliar fertilizer according to any one of claims 1 to 5, characterized in that: The following steps are involved: By simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency. Adjust the carrier modification process and functional component loading parameters based on the surface functional group characteristics of the nanocarrier and the metabolic activity of the microorganisms; Adopt online monitoring and feedback adjustment system to ensure the batch stability of nanocarrier structure, microbial activity and release curve in batch production; Evaluate the nutrient retention and ecological safety of products under adverse conditions through salt stress, drought simulation and leaching experiments; Integrate near-infrared spectroscopy (NIR), flow cytometers, and manufacturing execution systems (MES) to achieve full quality traceability and dynamic control from raw materials to finished products. The crop microenvironment includes pH, temperature and humidity.

7. The quality control method of the plasma nano-amino acid foliar fertilizer according to claim 6, characterized in that: By simulating the crop microenvironment, the nutrient release kinetics of the nanocarriers under different environmental parameters were measured to verify their response threshold and release efficiency, including: pH gradient test, set 5 gradient intervals of 1.0 in the pH range of 3.0-9.0, and measure the nutrient release rate over 24 hours; Temperature gradient test: set 5 gradients with intervals of 5°C in the range of 20°C-40°C to measure the nutrient release rate; Humidity simulation: Control the humidity between 40% and 90% RH with saturated salt solution to verify the moisture absorption and release balance of the carrier; The release curve was fitted using the Korsmeyer-Peppas equation, the release index was calculated, and the release mechanism was determined to be: Diffusion control n<0.45, skeleton dissolution 0.45 <n<0.89。 The release efficiency benchmark is set as a release rate of ≥60% in the pH range of 5.5-6.8 and a release rate of ≥80% over 24 hours at 30°C.

8. The quality control method of the plasma nano-amino acid foliar fertilizer according to claim 6, characterized in that: Based on the characteristics of the functional groups on the nanocarrier surface and the metabolic activity of the microorganisms, the carrier modification process and functional component loading parameters are adjusted, including: The surface -NH2 functional group content was determined by X-ray photoelectron spectroscopy (XPS), with a target value of 1.2-1.5 mmol / gSiO2; Zeta potential was verified using electrophoretic light scattering (ELS), with a target value >-30 mV to ensure pH response sensitivity. Immobilized bacterial agent loading ≥1×10 10 CFU / g carrier, and the viable bacteria rate ≥90%; Functional gene expression verification: qPCR was used to detect the expression of the phoD gene of phosphate-solubilizing bacteria, with a target value of ΔCt≤2.0; The freeze-drying protective agent formula is 20% trehalose and 10% glycerol.

9. The quality control method of the plasma nano-amino acid foliar fertilizer according to claim 6, characterized in that: Online monitoring and feedback control systems are used to ensure batch stability of nanocarrier structure, microbial activity, and release profiles during mass production, including: Near infrared spectroscopy (NIR) was used to monitor the functional groups of nanocarriers in real time, and the -NH2 peak intensity fluctuation was set to ≤5%. Flow cytometry was used to dynamically detect microbial activity, with a viable bacterial rate set at ≥95% and sampling every 30 minutes; The MES system is linked to the pH / temperature sensor to automatically calibrate the reactor stirring speed and set a ±5% fluctuation warning; If the release curve deviates from the preset model by more than 10%, a shutdown alarm is triggered and the abnormal batch is locked.

10. The quality control method of the plasma nano-amino acid foliar fertilizer according to claim 6, characterized in that: Through salt stress, drought simulation and leaching experiments, we evaluate the nutrient retention rate and ecological safety of products under adverse conditions, including: Salt stress test, NaCl concentration gradient 0-300mM, determination of chlorophyll fluorescence parameter Fv / Fm recovery rate ≥ 90%; Drought simulation, PEG 6000 concentration gradient 0-20%, verifying the sustained nutrient release rate of ≤15% over 7 days; Leaching experiment, Ag + Mobility detection ICP-MS method, set the target value <0.1mg / L; The effects on soil microbial communities were analyzed by high-throughput sequencing. The Shannon index of soil bacterial diversity was set to decrease by ≤10% after application.

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