Liver organ model culture device and culture method
By designing a liver organoid model culture device with liquid exchange holes for the injection and aspiration tubes, as well as a flow-promoting component and a flow-blocking plate, the problems of sudden temperature changes and gel matrix block damage during culture medium replacement were solved, achieving efficient and stable culture medium replacement and improving the culture effect.
Patent Information
- Application Number
- CN202510989913.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-17
- Publication Date
- 2025-10-28
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the process of culturing liver organoid models, the existing technology is prone to causing sudden temperature changes and damage to the gel matrix when changing the culture medium, which affects the culture effect.
A liver organoid model culture device was designed. The culture medium is replaced by inserting the injection tube and the aspiration tube into the temperature control box through the liquid replacement hole. The design of the flow propulsion component and the flow baffle plate enables the liquid replacement operation without the need to fully open the temperature control box, avoiding sudden temperature changes. The gel matrix block is protected by the curved tube opening and the drainage funnel structure.
It effectively prevents sudden temperature changes, improves culture results, saves medium change time, increases medium change efficiency, and avoids damage to the gel matrix block, thus ensuring culture results.
Smart Images
Figure CN120843273A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of cell culture, and more particularly to a liver organoid model culture device and culture method. Background Technology
[0002] Liver organoid culture is a type of micro-organ model constructed using in vitro three-dimensional culture technology to simulate the structure and function of liver tissue. Its core is to simulate the in vivo liver microenvironment to induce cells to self-assemble into a three-dimensional structure with multiple cell types such as hepatocytes and bile duct cells, and possess some liver functions.
[0003] During the culture process, in order to ensure sufficient supply of cell nutrition, the liquid culture medium needs to be replaced regularly. However, the temperature control chamber needs to be fully turned on to replace the culture medium in the culture dishes placed inside the temperature control chamber. This process can easily cause a sudden change in temperature around the culture dishes, which will affect the culture. At the same time, the gel matrix block is easily damaged due to suction and other factors during the replacement of the culture medium, which further affects the culture effect. Summary of the Invention
[0004] The purpose of this invention is to address the shortcomings of the prior art by proposing a liver organoid model culture device and culture method.
[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a liver organoid model culture device, comprising a temperature control chamber and a cover disposed on the temperature control chamber. A pressing column is slidably installed through the middle of the cover. A column spring is wound around the outside of the pressing column. One end of the column spring is connected to the upper end of the pressing column, and the other end of the column spring is fixed to the upper end of the cover. A culture element is disposed on the upper end of the temperature control chamber, located inside the cover. Multiple liquid exchange holes are opened through the upper end of the cover. A support frame is placed on the upper end of the cover. Multiple injection tubes and multiple suction tubes are embedded through the support frame. The injection tubes are located to the side of the suction tubes. The lower end of the injection tube is bent. Both the suction tubes and the injection tubes are inserted into the liquid exchange holes. The suction tubes are lower than the injection tubes. A flow-propelling element is disposed inside the injection tubes and the suction tubes. Two sealing plates are symmetrically installed on the top surface inside the cover.
[0006] Preferably, slots extend from both sides of the box cover, and inserts are fixedly installed on both sides of the support frame, with the inserts fitting into the slots.
[0007] Preferably, two plate shafts are symmetrically arranged on the inner top surface of the box cover, the sealing plate is fixed to the plate shafts, a bearing seat is rotatably installed on the outer surface of the plate shaft near the edge, the upper end of the bearing seat is fixed to the box cover, and a torsion spring is fixedly installed between the end of the plate shaft and the bearing seat.
[0008] Preferably, the propulsion component includes multiple No. 1 pistons disposed inside multiple injection tubes, and No. 2 pistons disposed inside each of the multiple suction tubes. An upper rod frame is fixedly installed between the upper ends of the multiple No. 1 pistons, and a lower rod frame is fixedly installed between the upper ends of the multiple No. 2 pistons. The lower rod frame is located below the upper rod frame. L-shaped frames are fixedly installed on both sides of the upper end of the support frame. A control gear is rotatably installed at the end of the L-shaped frame. A lower toothed plate meshes with one side of the control gear and is fixed to the lower rod frame. An upper toothed plate meshes with the other side of the control gear and is fixed to the upper rod frame. A handle is fixedly installed at the middle of the upper end of the upper rod frame.
[0009] Preferably, the culture medium includes a culture plate placed on the upper end of a temperature-controlled chamber, a chamber cover covering the outside of the culture plate, multiple culture tanks evenly distributed and fixedly installed on the upper end of the culture plate, a drainage hopper fixedly connected to the side of the culture tank, multiple receiving grooves fixedly installed on the upper end of the culture plate near the culture tank, the drainage hopper being connected to the receiving grooves, the bottom upper edge of the drainage hopper being flush with the top surface of the gel matrix block, the lower end of the injection tube being located inside the culture tank, and the lower end of the aspiration tube being located inside the receiving groove.
[0010] Preferably, a positioning block is fitted at the corner of the outer surface of the culture plate, the lower end of the positioning block is fixed to the temperature control box, a column sleeve is fixedly installed at the middle of the upper end of the culture plate, a push plate column is slidably installed inside the column sleeve, the push plate column extends through the upper end of the column sleeve, the push plate column is aligned with the pressing column, two protruding ears are symmetrically fixedly installed at the upper end of the push plate column, a pull plate is provided at both the front and rear edges of the upper end of the culture plate, a push plate frame is rotatably connected between the two protruding ears and the two pull plates, and multiple flow-blocking plates are linearly arrayed on the opposite surfaces of the two pull plates, the flow-blocking plates are inserted tightly into the drainage hopper.
[0011] Preferably, two convex guide posts are symmetrically fixedly installed on opposite sides of the two pull plates. A guide shell is slidably installed on the outer surface of the convex guide post. The guide shell is fixed to the culture plate. A return spring is fixedly installed between the inner end face of the guide shell and the end of the convex guide post.
[0012] A method for culturing liver organoid models is also provided, including the following steps:
[0013] S1: Mix liver progenitor cells with a basement membrane extract rich in collagen and laminin, and add the mixture dropwise into a culture tank;
[0014] S2: The culture plate is then placed in a temperature-controlled chamber at 37 degrees Celsius to allow the mixture in the culture tank to solidify and form a gel matrix block, providing a three-dimensional scaffold for the cells.
[0015] S3: Then insert the injection tube and aspiration tube into the box cover through the liquid exchange hole to add culture medium on top of the gel matrix block and perform subsequent culture medium replacement operations, thereby forming a three-dimensional environment for embedding and suspension for culture.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] 1. Through the set liquid exchange hole, the injection tube and the suction tube can be inserted into the box cover through the liquid exchange hole. During this process, the injection tube and the suction tube will push the sealing plate of the liquid exchange hole, so that the sealing plate flips down with the plate axis as the center, allowing the injection tube and the suction tube to be smoothly inserted into the box cover for liquid exchange operation. This process avoids the temperature control box being fully opened, effectively preventing sudden temperature changes and improving the culture effect.
[0018] 2. By pressing the pressing column, the push plate column is pushed downwards, which in turn moves the push plate frame to push the pull plate, allowing the flow-blocking plate on the pull plate to be pulled out from the drainage funnel. This opens the drainage funnel, allowing the liquid culture medium on the gel matrix block in the culture tank to flow into the receiving tank through the drainage funnel. Then, releasing the pressing column resets the flow-blocking plate. Next, pressing the handle lowers the first piston on the upper rod, injecting the culture medium from the injection tube into the gel matrix block in the culture tank. During this process, the lowering of the upper rod causes the upper toothed plate to move downwards, which in turn moves the lower toothed plate upwards via the control gear. This causes the second piston on the lower rod to move upwards, drawing the replaced culture medium flowing into the receiving tank into the suction tube, thus completing the process. The medium replacement process has several advantages. First, it allows for the simultaneous replacement of culture media in multiple culture tanks. It also enables the removal of old media while new media is being injected, effectively saving time and improving efficiency. Second, by channeling the liquid culture media from the gel matrix blocks within the culture tank into the receiving tank via a drainage funnel, it ensures that the suction force during subsequent media removal does not affect the gel matrix blocks, effectively preventing damage. Furthermore, the curved design of the injection tube pushes the culture media against the inner wall of the culture tank, allowing it to flow along the wall onto the gel matrix blocks, thus avoiding direct impact and damage. This further enhances the culture effect. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the structure of a liver organoid model culture device according to the present invention;
[0020] Figure 2 This is an exploded view of the support frame and box lid of a liver organoid model culture device according to the present invention;
[0021] Figure 3 This invention relates to a liver organoid model culture device. Figure 2 A magnified view of middle A;
[0022] Figure 4 This invention relates to a liver organoid model culture device. Figure 2 Enlarged view of middle B;
[0023] Figure 5 This is a schematic diagram of the support frame of the liver organoid model culture device of the present invention;
[0024] Figure 6 This is a schematic diagram of the insertion point of a liver organoid model culture device according to the present invention;
[0025] Figure 7 This is a schematic diagram of the culture plate of a liver organoid model culture device according to the present invention;
[0026] Figure 8 This is a schematic diagram of the culture tank of a liver organoid model culture device according to the present invention;
[0027] Figure 9 This is a schematic diagram from another perspective of the culture tank of the liver organoid model culture device of the present invention;
[0028] Figure 10 This is an internal view of the guide shell of a liver organoid model culture device according to the present invention;
[0029] Figure 11 This is a view of the inner top surface of the lid of the liver organoid model culture device of the present invention.
[0030] In the diagram: 1. Temperature control chamber; 2. Chamber lid; 3. Culture plate; 4. Support frame; 5. Slot; 6. Insertion frame; 7. Pressing column; 8. Column spring; 9. L-shaped frame; 10. Liquid changing hole; 11. Sealing plate; 12. Plate shaft; 13. Shaft seat; 14. Torsion spring; 15. Positioning block; 16. Guide shell; 17. Receiving groove; 18. Pull plate; 19. Push plate column; 20. Extending ear; 21. Push plate frame; 22. Column sleeve; 23. Culture tank; 24. Return spring; 25. Protruding guide column; 26. Baffle plate; 27. Drainage funnel; 28. Handle; 29. Upper rod frame; 30. Suction tube; 31. Injection tube; 32. Piston No. 1; 33. Piston No. 2; 34. Lower rod frame; 35. Upper gear plate; 36. Lower gear plate; 37. Control gear. Detailed Implementation
[0031] The following description is intended to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are merely examples, and those skilled in the art may conceive of other obvious variations.
[0032] like Figures 1-11 The illustrated liver organoid model culture device includes a temperature control chamber 1 and a cover 2 mounted on the temperature control chamber 1. The cover 2 is detachably connected to the temperature control chamber 1, allowing the cover 2 to be removed to place a culture plate 3 onto the temperature control chamber 1. A pressing post 7 is slidably mounted through the center of the cover 2, and a spring 8 is wound around the outside of the pressing post 7. One end of the spring 8 is connected to the upper end of the pressing post 7, and the other end of the spring 8 is fixed to the upper end of the cover 2. The spring 8 serves to reset the pressing post 7. The function is as follows: the upper part of the temperature control chamber 1, located inside the chamber cover 2, is equipped with culture media. Multiple liquid exchange holes 10 are provided through the upper part of the chamber cover 2. The injection tube 31 and suction tube 30 are inserted into the chamber cover 2 through the liquid exchange holes 10. During this process, the injection tube 31 and suction tube 30 push the sealing plate 11 that seals the liquid exchange holes 10, causing the sealing plate 11 to flip downwards around the plate shaft 12, allowing the injection tube 31 and suction tube 30 to be smoothly inserted into the chamber cover 2 for liquid exchange. This process avoids fully opening the temperature control chamber. 1. Effectively prevents sudden temperature changes and improves the cultivation effect. A support frame 4 is placed on the upper end of the box cover 2. Multiple injection tubes 31 and multiple suction tubes 30 are embedded through the support frame 4. The injection tubes 31 are located to the side of the suction tubes 30. The support frame 4 serves to support the suction tubes 30 and injection tubes 31. The lower end of the injection tube 31 is bent, which can push the culture medium towards the inner wall of the culture tank 23, allowing the culture medium to flow along the inner wall of the culture tank 23. The liquid is placed onto the gel matrix block to avoid direct impact from the culture medium and damage to the gel matrix block. The aspirator 30 and the injection tube 31 are both inserted into the liquid exchange hole 10. The aspirator 30 is lower than the injection tube 31, which can adapt to the height difference between the bottom of the gel matrix block in the culture tank 23 and the bottom of the receiving tank 17 for liquid exchange. The inside of the injection tube 31 and the inside of the aspirator 30 are provided with a flow propulsion component. Two sealing plates 11 are symmetrically installed on the top surface of the inside of the box cover 2. The sealing plates 11 serve to seal the liquid exchange hole 10.
[0033] Both sides of the cover 2 have slots 5 extending outwards, and both sides of the support frame 4 have inserts 6 fixedly installed. The cooperation between the slots 5 and the inserts 6 can guide the support frame 4 so that the injection tube 31 and the suction tube 30 can pass through the liquid exchange hole 10. The inserts 6 are inserted into the inside of the slots 5.
[0034] Two plate shafts 12 are symmetrically arranged on the inner top surface of the box cover 2. The sealing plate 11 is fixed to the plate shafts 12, and the plate shafts 12 serve to support the sealing plate 11. A bearing seat 13 is rotatably installed on the outer surface of the plate shaft 12 near the edge. The upper end of the bearing seat 13 is fixed to the box cover 2, and the bearing seat 13 serves to connect the plate shaft 12. A torsion spring 14 is fixedly installed between the end of the plate shaft 12 and the bearing seat 13. After the injection tube 31 and the suction tube 30 are pulled out from the liquid exchange hole 10, the torsion spring 14 will push the sealing plate 11 to reset it so that it can be reattached to the inner top surface of the box cover 2 to seal the liquid exchange hole 10.
[0035] The propulsion component includes multiple primary pistons 32 disposed inside multiple injection tubes 31. The primary pistons 32 inject the culture medium from the injection tubes 31 above the gel matrix block in the culture tank 23. Multiple aspiration tubes 30 each have secondary pistons 33 disposed inside. The secondary pistons 33 draw the replaced culture medium flowing into the receiving tank 17 into the aspiration tubes 30. An upper rod 29 is fixedly installed between the upper ends of the primary pistons 32, facilitating synchronous movement of the primary pistons 32. A lower rod 34 is fixedly installed between the upper ends of the secondary pistons 33, facilitating synchronous movement of the secondary pistons 33. The lower rod 34 is located below the upper rod 29. L-shaped frames 9 are fixedly installed on both sides of the upper end of the support frame 4. Control gears 37 are rotatably mounted at the ends of the L-shaped frames 9, supporting the control gears 37. A lower toothed plate 36 is engaged on one side and fixed to a lower rod 34. An upper toothed plate 35 is engaged on the other side of a control gear 37 and fixed to an upper rod 29. A handle 28 is fixedly installed at the middle of the upper end of the upper rod 29. Pressing the handle 28 will cause the first piston 32 on the upper rod 29 to move down, so as to inject the culture medium in the injection tube 31 into the gel matrix block in the culture tank 23. During this process, the moving upper rod 29 will drive the upper toothed plate 35 to move down, and then drive the lower toothed plate 36 to move up through the control gear 37, so as to move the second piston 33 on the lower rod 34 to move up, so as to suck the replaced culture medium flowing into the receiving tank 17 into the suction tube 30, thereby completing the culture medium replacement. This process can replace the culture medium in multiple culture tanks 23 at the same time, and can also simultaneously suck away the replaced culture medium while injecting new culture medium, effectively saving time and improving the culture medium replacement efficiency.
[0036] The culture medium includes a culture plate 3 placed on top of a temperature-controlled chamber 1, with a chamber cover 2 covering the outside of the culture plate 3. Multiple culture tanks 23 are evenly distributed and fixedly installed on the upper end of the culture plate 3. The culture tanks 23 contain the gel matrix blocks and culture medium. A drainage funnel 27 is fixedly connected to the side of the culture tank 23. Multiple receiving grooves 17 are fixedly installed on the upper end of the culture plate 3 near the side of the culture tank 23. The drainage funnel 27 serves to guide the flow of culture medium. The drainage funnel 27 is connected to the receiving grooves 17, and the bottom upper edge of the drainage funnel 27 is flush with the top surface of the gel matrix block, facilitating the flow of culture medium from above the gel matrix block. The lower end of the injection tube 31 is located inside the culture tank 23, and the lower end of the suction tube 30 is located inside the receiving groove 17. By allowing the liquid culture medium on the gel matrix block in the culture tank 23 to flow into the receiving groove 17 through the drainage funnel 27, it is ensured that the suction force will not affect the gel matrix block when the culture medium is subsequently aspirated, effectively preventing damage to the gel matrix block.
[0037] A positioning block 15 is fitted to the corner of the outer surface of the culture plate 3. The lower end of the positioning block 15 is fixed to the temperature control box 1, and the positioning block 15 serves to position the culture plate 3. A column sleeve 22 is fixedly installed in the middle of the upper end of the culture plate 3. A push plate column 19 is slidably installed inside the column sleeve 22. The push plate column 19 extends through the upper end of the column sleeve 22 and serves to guide the push plate column 19. The push plate column 19 is aligned with the pressing column 7. Two protruding ears 20 are symmetrically fixedly installed at the upper end of the push plate column 19. The protruding ears 20 serve to connect. Pull plates 18 are provided at the front and rear edges of the upper end of the culture plate 3. The two protruding ears 20 are connected to the pull plates 18. A push plate frame 21 is rotatably connected between the two pull plates 18. Multiple flow-blocking plates 26 are arranged linearly on the opposite surfaces of the two pull plates 18. The flow-blocking plates 26 can block the drainage hopper 27 to prevent the culture medium from flowing out. The flow-blocking plates 26 are inserted tightly into the drainage hopper 27. By pressing the pressing column 7, the push plate column 19 can be pushed down by the pressing column 7, which in turn drives the push plate frame 21 to move, thereby pushing the pull plates 18 so that the flow-blocking plates 26 on the pull plates 18 can be pulled out from the drainage hopper 27, allowing the drainage hopper 27 to be opened, so that the liquid culture medium on the gel matrix block in the culture tank 23 can flow into the receiving tank 17 through the drainage hopper 27.
[0038] Two convex guide posts 25 are symmetrically fixedly installed on opposite sides of the two pull plates 18. A guide shell 16 is slidably installed on the outer surface of the convex guide post 25. The convex guide post 25 and the guide shell 16 serve to guide the pull plates 18. The guide shell 16 is fixed to the culture plate 3. A return spring 24 is fixedly installed between the inner end face of the guide shell 16 and the end of the convex guide post 25. The return spring 24 can push the pull plate 18 to reset the flow-blocking plate 26 on the pull plate 18 so that it can be re-inserted into the drainage hopper 27 for sealing.
[0039] A method for culturing liver organoid models is also provided, including the following steps:
[0040] S1: Mix liver progenitor cells with a basement membrane extract rich in collagen and laminin, and add the mixture dropwise into culture tank 23;
[0041] S2: Then the culture plate 3 is placed in the temperature control box 1 to allow the mixture in the culture tank 23 to solidify into a gel matrix block at 37 degrees Celsius, so as to provide a three-dimensional scaffold for the cells.
[0042] S3: Then, insert the injection tube 31 and the aspiration tube 30 into the box cover 2 through the liquid exchange hole 10 to add culture medium on top of the gel matrix block and perform subsequent culture medium replacement operations, thereby forming a three-dimensional environment for embedding and suspension for culture.
[0043] During culture medium replacement, press the pressing column 7 to push the push plate column 19 downwards, which in turn moves the push plate frame 21 to push the pull plate 18, allowing the flow-blocking plate 26 on the pull plate 18 to be pulled out from the drainage funnel 27, thus opening the drainage funnel 27. This allows the liquid culture medium on the gel matrix block in the culture tank 23 to flow into the receiving tank 17 through the drainage funnel 27. Then, release the pressing column 7 to reset the flow-blocking plate 26. Next, insert the injection tube 31 and the suction tube 30 through the culture medium replacement hole 10 into the box cover 2. During this process, the injection tube 31 and the suction tube 30 will push the sealing plate 11 that seals the culture medium replacement hole 10, causing the sealing plate 11 to flip downwards around the plate shaft 12, allowing the injection tube to... 31. The aspirator 30 is successfully inserted into the cover 2. At this time, the inlet of the injection tube 31 inserted into the cover 2 will extend into the culture tank 23, and the inlet of the aspirator 30 will extend into the receiving tank 17. At the same time, the support frame 4 is attached to the upper end of the cover 2. Then, press the handle 28 to move the first piston 32 on the upper rod 29 downward to inject the culture medium in the injection tube 31 into the gel matrix block in the culture tank 23. During this process, the downward moving upper rod 29 will drive the upper toothed plate 35 downward, and then drive the lower toothed plate 36 upward through the control gear 37, so that the second piston 33 on the lower rod 34 will move upward to suck the replaced culture medium flowing into the receiving tank 17 into the aspirator 30, thus completing the culture medium replacement.
[0044] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention. The scope of protection claimed by the appended claims and their equivalents is defined.
Claims
1. A liver organoid model culture device, comprising a temperature control chamber (1) and a chamber cover (2) disposed on the temperature control chamber (1), characterized in that: A pressing post (7) is slidably installed through the middle of the box cover (2). A column spring (8) is wound around the outside of the pressing post (7). One end of the column spring (8) is connected to the upper end of the pressing post (7), and the other end of the column spring (8) is fixed to the upper end of the box cover (2). A culture medium is provided on the upper end of the temperature control box (1) inside the box cover (2). Multiple liquid exchange holes (10) are opened through the upper end of the box cover (2). A support frame (4) is placed on the upper end of the box cover (2). A support frame (4) is embedded through the support frame (4). There are multiple injection tubes (31), and multiple suction tubes (30) are embedded through the support frame (4). The injection tube (31) is located on the side of the suction tube (30). The lower end of the injection tube (31) is bent. The suction tube (30) and the injection tube (31) are both inserted into the liquid exchange hole (10). The suction tube (30) is lower than the injection tube (31). The inside of the injection tube (31) and the inside of the suction tube (30) are provided with a flow-pushing element. Two sealing plates (11) are symmetrically installed on the top surface of the inside of the box cover (2).
2. The liver organoid model culture device according to claim 1, characterized in that: The box cover (2) has slots (5) extending from both sides, and the support frame (4) has inserts (6) fixedly installed on both sides, with the inserts (6) inserted into the slots (5).
3. The liver organoid model culture device according to claim 2, characterized in that: Two plate shafts (12) are symmetrically arranged on the inner top surface of the box cover (2). The sealing plate (11) is fixed to the plate shafts (12). A bearing seat (13) is rotatably installed on the outer surface of the plate shaft (12) near the edge. The upper end of the bearing seat (13) is fixed to the box cover (2). A torsion spring (14) is fixedly installed between the end of the plate shaft (12) and the bearing seat (13).
4. The liver organoid model culture device according to claim 3, characterized in that: The propulsion component includes multiple first pistons (32) disposed inside multiple injection tubes (31), and multiple second pistons (33) disposed inside multiple suction tubes (30). An upper rod frame (29) is fixedly installed between the upper ends of the multiple first pistons (32), and a lower rod frame (34) is fixedly installed between the upper ends of the multiple second pistons (33). The lower rod frame (34) is located below the upper rod frame (29), and the upper end of the support frame (4) is... Both sides are fixedly installed with L-shaped frames (9). A control gear (37) is rotatably installed at the end of the L-shaped frame (9). A lower toothed plate (36) meshes with one side of the control gear (37). The lower toothed plate (36) is fixed to the lower rod frame (34). An upper toothed plate (35) meshes with the other side of the control gear (37). The upper toothed plate (35) is fixed to the upper rod frame (29). A handle (28) is fixedly installed at the middle of the upper end of the upper rod frame (29).
5. The liver organoid model culture device according to claim 4, characterized in that: The culture medium includes a culture plate (3) placed on the upper end of a temperature control box (1). The box cover (2) covers the outside of the culture plate (3). Multiple culture tanks (23) are evenly distributed and fixedly installed on the upper end of the culture plate (3). A drainage bucket (27) is fixedly connected to the side of the culture tank (23). Multiple receiving grooves (17) are fixedly installed on the side of the upper end of the culture plate (3) near the culture tank (23). The drainage bucket (27) is connected to the receiving groove (17). The bottom upper edge of the drainage bucket (27) is flush with the top surface of the gel matrix block. The lower end of the injection tube (31) is located in the culture tank (23). The lower end of the suction tube (30) is located in the receiving groove (17).
6. The liver organoid model culture device according to claim 5, characterized in that: A positioning block (15) is attached to the corner of the outer surface of the culture plate (3). The lower end of the positioning block (15) is fixed to the temperature control box (1). A column sleeve (22) is fixedly installed in the middle of the upper end of the culture plate (3). A push plate column (19) is slidably installed inside the column sleeve (22). The push plate column (19) extends through the upper end of the column sleeve (22). The push plate column (19) is aligned with the pressing column (7). Two protruding ears (20) are symmetrically fixedly installed at the upper end of the push plate column (19). Pull plates (18) are provided at the front and rear edges of the upper end of the culture plate (3). A push plate frame (21) is rotatably connected between the two protruding ears (20) and the two pull plates (18). Multiple flow-blocking plates (26) are linearly arrayed on the opposite surfaces of the two pull plates (18). The flow-blocking plates (26) are inserted tightly into the drainage hopper (27).
7. The liver organoid model culture device according to claim 6, characterized in that: Two convex guide posts (25) are symmetrically fixedly installed on opposite sides of the two pull plates (18). A guide shell (16) is slidably installed on the outer surface of the convex guide post (25). The guide shell (16) is fixed to the culture plate (3). A return spring (24) is fixedly installed between the inner end face of the guide shell (16) and the end of the convex guide post (25).
8. A method for culturing a liver organoid model, applied in the liver organoid model culture apparatus of claim 7, characterized in that, The following steps are involved: S1: Mix liver progenitor cells with a basement membrane extract rich in collagen and laminin, and add it dropwise into a culture tank (23); S2: The culture plate (3) is then placed in the temperature control box (1) to allow the mixture in the culture tank (23) to solidify into a gel matrix block at 37 degrees Celsius, so as to provide a three-dimensional scaffold for the cells. S3: Then insert the injection tube (31) and the suction tube (30) into the box cover (2) through the liquid exchange hole (10) to add culture medium on top of the gel matrix block and to perform subsequent culture medium replacement operations, thereby forming an embedded and suspended three-dimensional environment for culture.