Multifunctional fixing device for mouse ventricular microinjection and use method

Abstract

The invention discloses a multifunctional fixing device for mouse ventricular micro-injection and a using method. The multifunctional fixing device comprises an operation table, a height adjusting assembly, a fixing plate, a cranial height adjusting frame, a mounting base, an incisor hook and an ear rod. Wherein the operation table comprises a bottom plate and a vertical plate which are vertically connected, a sinking groove is formed in the bottom plate, and a lifting table is arranged in the groove; the height adjusting assembly is parallel to the vertical plate and close to the bottom plate side; the fixed plate is provided with two symmetrical extension strips at the moving end of the adjusting assembly and on the deviating side, the other surface of the fixed plate is provided with a length adjusting groove, and a first driving piece is arranged in the groove; the cranial height adjusting frame is connected with the first driving piece and provided with a second driving piece. A fixing hole is formed in the far end of the mounting seat; and the incisor hook is arranged on the mounting seat and is positioned above the fixing hole. The two ear rods are symmetrically arranged at the far end of the extension strip. The scheme has the effects of reducing damage to mice, reducing experimental pollution and improving operation stability.

Description

Technical Field

[0001] This application relates to the field of animal experimental instrument technology, specifically to a multifunctional fixation device and its method of use for microinjection into the ventricles of mice. Background Technology

[0002] Intraventricular microinjection in mice is a key technique for the precise delivery of drugs, viral vectors, or tracers into the ventricles of mice, while the fixation device is a core component that ensures the accuracy and stability of this technique.

[0003] Currently, existing fixation devices for intraventricular microinjection in mice are mainly divided into two parts: a head fixation module and a body fixation module. The head fixation module often uses a combination of ear rod clamping and incisor hook limiting structure. By adjusting the distance between the ear rods to match the width of the mouse's head, the incisor hook is used to fix the mouse's incisors to limit the forward and backward displacement of the head, ensuring that the position of the mouse's head is accurately aligned with the coordinate system of the stereotaxic instrument during the injection process. The body fixation module is mostly an integrated groove structure. After the mouse body is placed in, it is assisted by elastic straps or side baffles to avoid the mouse body shaking during the injection operation, which would interfere with the positioning accuracy. However, existing fixation devices still have significant shortcomings in practical applications and are difficult to meet diverse experimental needs.

[0004] On the one hand, the problem of poor head fixation adaptability is particularly prominent. There are significant deviations in the head proportions of mice of different strains and ages. Traditional head fixation components cannot accurately adjust the fixation position according to the head-mouth ratio, which may result in unstable fixation during experiments. Mice may also experience stress reactions due to discomfort in the fixation position, which can easily cause harm to the mice. On the other hand, the low efficiency of cleaning the operating table severely impacts experimental procedures. In mouse intraventricular microinjection, mice may urinate or defecate due to stress, or leakage of medication or tissue fluid may occur due to operational errors during injection. These contaminants can directly adhere to the operating table containing the fixation device. In existing technologies, the fixation device and operating table are often fixedly connected or integrated. When the operating table becomes contaminated, researchers must stop the current experiment and use sterile swabs dipped in alcohol or disinfectant to clean the gaps between the operating table and the fixation device. This process is not only cumbersome and time-consuming but also carries the risk of cross-contamination in subsequent experiments due to incomplete cleaning, reducing experimental efficiency and data reliability. Furthermore, repeated disassembly and cleaning may affect the structural stability of the fixation device, further increasing the risk of experimental errors and failing to meet the demands of high-throughput, high-precision neuroscience experiments. During the experiment, researchers typically had to hand-hold the drill bit when drilling holes in the mouse skull. However, this operation requires a high level of experience and precise control from the researchers. Novices often lack the expertise to control the drilling force and angle, and struggle to accurately locate the drilling position based on the mouse's varying head proportions, such as skull width, skull height, and ear spacing. This can lead to brain damage and death due to positioning errors, and may also cause the worktable to become contaminated with bodily fluids, tissue debris, or bone fragments from the drilling process during the operation. This further increases the cleaning burden on the worktable and the risk of cross-contamination, impacting the continuity of the experimental procedure and the reliability of the data. Summary of the Invention

[0005] Therefore, this application provides a multifunctional fixation device and method for use in mouse ventricular microinjection, in order to solve the problem of poor head fixation adaptability in the prior art.

[0006] To achieve the above objectives, this application provides the following technical solution: In a first aspect, a multifunctional fixation device for intraventricular microinjection in mice includes an operating table, a height adjustment component, a fixation plate, a skull height adjustment frame, a mounting base, incisor hooks, and ear rods. The operating table includes a vertically arranged base plate and a vertical plate, the vertical plate being connected to one side of the base plate. A recessed groove is formed on the top surface of the base plate, and a lifting platform is disposed within the recessed groove. The height adjustment component is arranged parallel to the vertical plate on the side of the vertical plate closest to the base plate. The fixation plate is located at the movable end of the height adjustment component, the height adjustment component providing lifting driving force to the fixation plate. Two extension strips are symmetrically arranged on the side of the fixation plate away from the height adjustment component, and the two extension strips are located at the movable end of the lifting platform. Above the lowering platform, a length adjustment groove is provided on the top surface of the fixed plate, and a first driving component is provided in the length adjustment groove; a head height adjustment frame is installed in the length adjustment groove and connected to the first driving component, the first driving component provides the head height adjustment frame with driving force to move closer to and away from the lifting platform, and a second driving component is provided on the head height adjustment frame; a mounting base is connected to the second driving component, the second driving component provides the mounting base with lifting driving force, and a fixing hole is provided at the end of the mounting base away from the head height adjustment frame; a front tooth hook is provided on the mounting base and located above the fixing hole; two ear rods are symmetrically provided at the ends of the two extension strips away from the fixed plate and are detachably connected to the extension strips.

[0007] Optionally, a counterweight plate is provided on the side of the base plate facing away from the lifting platform.

[0008] Optionally, it also includes an anti-fouling component, which includes a release roller, a recovery box, and a recovery element; wherein, the release roller is disposed on the counterweight plate, located on one side of the base plate, and a protective film is wound around the release roller; the recovery box is disposed on the counterweight plate, located on the side of the base plate away from the release roller, and the recovery box has an opening on the side near the base plate; the recovery element is disposed at the opening and is used to recover the protective film released by the release roller.

[0009] Optionally, the recycling component includes a recycling roller, a driven gear, and a driving gear; wherein, two recycling rollers are spaced apart along the height direction of the opening and are rotatably connected to the recycling box; the driven gear is located at one end of the recycling roller; the driving gear meshes with the driven gear, and a recycling motor is coaxially connected to the end of the driving gear away from the recycling roller.

[0010] Optionally, the multifunctional fixing device further includes a three-coordinate positioning component, which includes a Z-axis mounting bracket, a Y-axis mounting bracket, and an X-axis drive component; wherein, the Z-axis mounting bracket is disposed on one side of the base plate, and the Z-axis mounting bracket is provided with the Z-axis drive component; the Y-axis mounting bracket is connected to the Z-axis drive component and has a sliding degree of freedom along the Z-axis mounting bracket, and the Y-axis mounting bracket is provided with the Y-axis drive component; the X-axis drive component is connected to the Y-axis drive component.

[0011] Optionally, the multifunctional fixing device further includes a mounting block, a fine-tuning gear, a moving block, a drill, a limiting clamp, and a syringe; wherein, the mounting block includes a first connecting surface and a second connecting surface, the first connecting surface is provided with a receiving groove, and guide grooves are symmetrically provided on both sides of the receiving groove, the mounting block is connected to the X-axis drive component, and the mounting block can move along the length direction of the X-axis drive component; the fine-tuning gear is rotatably connected in the receiving groove, and a knob is provided at one end; the moving block is provided with a rack on one side, and guide blocks are symmetrically provided on both sides of the rack, the guide blocks are slidably connected in the guide groove, and the rack and the fine-tuning gear mesh; the drill is detachably connected to the side of the moving block near the base plate; the limiting clamp is provided on the second connecting surface; the syringe is detachably connected to the limiting clamp.

[0012] Optionally, the lifting platform includes multiple lifting cylinders and a placement plate, with the multiple lifting cylinders evenly arranged in the sinking trough and the placement plate located at the pushing end of the multiple lifting cylinders.

[0013] Secondly, a method of using a multifunctional fixation device for microinjection into the ventricles of mice, applied to the multifunctional fixation device described in any embodiment of this application, the method comprising the following steps: S1: First, control the lifting platform to rise. Pull the protective film out from the release roller and stick it to the upper surface of the lifting platform. Continue to pull it out until one end of the protective film extends into the opening of the recycling box. The recycling roller will then drive the protective film into the recycling box to tighten the protective film. S2: Fix the mouse body on the protective film on the lifting platform. Adjust the height of the fixing plate according to the position of the mouse's ears so that it is at the same height as the mouse's ears. Then insert the ear rod into the mouse's ears. Next, adjust the height of the mounting base according to the position of the mouse's mouth so that the mouse's lower jaw is in the fixing hole and the incisor hook is inserted into the mouse's mouth to fix the mouse's head. S3: The experimenters began to make an opening in the mouse's head, and then marked the location of the opening on the mouse's skull with a marker. S4: The experimenter manipulates the three-coordinate positioning component to move the mounting block to the top of the mouse's head, keeping the drill bit of the drill against the marked position. At this time, the drill is turned on to drill a hole in the mouse's skull. The experimenter operates the knob to control the drill to move downward to complete the hole opening. Then, the experimenter operates the knob to control the drill to rise to a safe position. Next, the syringe is installed on the limit clamp, the three-coordinate positioning component is adjusted, and the syringe pillow is moved to the hole opening position. The drug in the syringe is injected into the mouse's ventricle. Then, the three-coordinate positioning component is manipulated to move the mounting block away from the area above the mouse. S5: The experimenter sutures the skin on the mouse's head. After suturing, the mouse is removed from the lifting platform. If no obvious contamination is caused on the protective film during the experiment, the recovery roller will carry the exposed part of the protective film into the recovery box. If obvious contamination is caused, the experimenter needs to clean the obvious contaminants on the protective film and then the recovery roller will carry the contaminated part of the protective film into the recovery box.

[0014] Compared with the prior art, this application has at least the following beneficial effects: The base plate and vertical plate of the operating table are vertically connected to form a stable basic support structure. The lifting platform in the sinking groove can be adjusted in height according to the size of the mouse, ensuring that the mouse's body is in a suitable position for head fixation. The height adjustment component can drive the fixing plate to move along the height direction, providing a height adaptation basis for the ear rod to accurately insert into the mouse's ear, avoiding head clamping deviation due to improper ear rod height. Two symmetrically arranged extension strips provide a symmetrical mounting carrier for the ear rod, ensuring that the clamping force of the ear rod on both sides of the mouse's head is balanced, preventing head tilting caused by unilateral force. The length adjustment groove cooperates with the first drive component to adjust the skull height according to the horizontal distance between the mouse's head and mouth. The first mounting position allows the incisor hook to be precisely aligned with the mouse's mouth; the second drive unit drives the mounting base to rise and fall, adapting to different mouse head heights and ensuring the incisor hook can smoothly enter the mouse's mouth. At the same time, the fixing hole of the mounting base limits the mouse's lower jaw. Combined with the clamping of the ear rod and the hooking of the incisor hook, it achieves precise fixation of the mouse's head from multiple directions, solving the problem of poor adaptability of traditional head fixation components and reducing experimental errors caused by mouse stress and insecure fixation. The ear rod and extension strip are detachably connected, making it easy to replace ear rods of different specifications according to the size of the mouse's ears, further improving the adaptability of the device and reducing the risk of damage to the mouse's ears while ensuring stable clamping. Attached Figure Description

[0015] To more intuitively illustrate the prior art and this application, several exemplary figures are provided below. It should be understood that the specific shapes and structures shown in the figures should not generally be regarded as limiting conditions for implementing this application; for example, based on the technical concept disclosed in this application and the exemplary figures, those skilled in the art are able to easily make conventional adjustments or further optimizations to the addition / reduction / classification, specific shapes, positional relationships, connection methods, size ratios, etc. of certain units (components).

[0016] Figure 1 A three-dimensional structural diagram of a multifunctional fixation device for intraventricular microinjection in mice; Figure 2 This is a partial structural diagram of a multifunctional fixation device used for intraventricular microinjection in mice. Figure 3 A frontal sectional view of the operating table of a multifunctional fixation device for intraventricular microinjection in mice. Figure 4 A three-dimensional cross-sectional view of the anti-fouling component of a multifunctional fixation device for microinjection into the ventricle of mice. Figure 5 A three-dimensional enlarged view of the mounting base structure of the multifunctional fixation device for mouse ventricle microinjection provided in an embodiment of this application; Figure 6 A three-dimensional structural schematic diagram of the three-coordinate positioning component of the multifunctional fixation device for mouse ventricular microinjection provided in the embodiments of this application; Figure 7 This is a three-dimensional disassembled view of the mounting block and connecting block in the multifunctional fixation device for mouse ventricle microinjection provided in the embodiments of this application.

[0017] Explanation of reference numerals in the attached figures: 1. Operating platform; 101. Base plate; 1011. Sinking trough; 102. Vertical plate; 103. Lifting platform; 1031. Placement plate; 1032. Lifting cylinder; 104. Counterweight plate; 2. Height adjustment assembly; 3. Fixing plate; 301. Extension strip; 302. Length adjustment groove; 303. First drive component; 4. Head height adjustment frame; 401. Second drive component; 5. Mounting base; 501. Fixing hole; 502. Inclined tooth hook; 503. Ear rod; 6. Anti-fouling assembly; 601. Release roller; 602. Recycling box; 603. Recycling component; 6031. Recycling roller; 60 32. Driven gear; 6033. Drive gear; 6034. Recycling motor; 604. Protective film; 7. Three-axis positioning assembly; 701. Z-axis mounting bracket; 702. Y-axis mounting bracket; 703. Z-axis drive component; 704. Y-axis drive component; 705. X-axis drive component; 8. Mounting block; 801. First connecting surface; 802. Second connecting surface; 803. Receiving groove; 804. Guide groove; 9. Fine-tuning gear; 901. Knob; 10. Moving block; 1001. Rack; 1002. Guide block; 11. Drill; 12. Limiting clamp; 13. Syringe. Detailed Implementation

[0018] The present application will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0019] In the description of this application: unless otherwise stated, "a plurality of" means two or more. The terms "first," "second," "third," etc., in this application are intended to distinguish the objects referred to and do not have any special meaning in terms of technical connotation (e.g., they should not be construed as an emphasis on importance or order). Expressions such as "including," "comprising," and "having" also mean "not limited to" (certain units, components, materials, steps, etc.).

[0020] The terms used in this application, such as "upper," "lower," "left," "right," and "middle," are generally used to facilitate intuitive understanding by referring to the accompanying drawings, and are not absolute limitations on the positional relationships in the actual product. Changes in these relative positional relationships, without departing from the technical concept disclosed in this application, should also be considered within the scope of this application.

[0021] The following is combined Figures 1 to 7 The illustrated embodiments describe the technical solution of the present invention: This invention provides a multifunctional fixation device for intraventricular microinjection in mice, such as... Figures 1 to 7As shown, the device includes an operating platform 1, a height adjustment assembly 2, a fixing plate 3, a head height adjustment frame 4, a mounting base 5, an incisor hook 502, and an ear rod 503. The operating platform 1 includes a vertically arranged base plate 101 and a vertical plate 102. The vertical plate 102 is connected to one side of the base plate 101. A recessed groove 1011 is formed on the base plate 101, and a lifting platform 103 is located within the recessed groove 1011. The height adjustment assembly 2 is arranged parallel to the vertical plate 102 on the side of the vertical plate 102 closest to the base plate 101. The fixing plate 3 is located at the moving end of the height adjustment assembly 2, and the height adjustment assembly 2 provides lifting driving force to the fixing plate 3. Two extension bars 301 are symmetrically arranged on the side of the fixing plate 3 away from the height adjustment assembly 2, and the two extension bars 301 are located above the lifting platform 103. The top surface of the fixed plate 3 is provided with a length adjustment groove 302, and a first driving member 303 is provided in the length adjustment groove 302. The head height adjustment frame is installed in the length adjustment groove 302 and connected to the first driving member 303. The first driving member 303 provides the head height adjustment frame 4 with driving force to move closer to and away from the lifting platform 103. The head height adjustment frame 4 is provided with a second driving member 401. The mounting base 5 is connected to the second driving member 401. The second driving member 401 provides the mounting base 5 with lifting driving force. The end of the mounting base 5 away from the head height adjustment frame 4 is provided with a fixing hole 501. The incisor hook 502 is provided on the mounting base 5 and is located above the fixing hole 501. Two ear rods 503 are symmetrically arranged at the ends of the two extension strips 301 away from the fixed plate 3 and are detachably connected to the extension strips 301.

[0022] The height adjustment component 2 includes a threaded rod, a guide rod, a servo motor, a sleeve, and a connecting plate. The connecting plate is connected to one side of the fixed plate 3 by bolts. Two sleeves are symmetrically connected to the side of the connecting plate away from the fixed plate 3. The threaded rod and the guide rod are spaced apart on the side of the vertical plate close to the bottom plate 101. Two sleeves are respectively fitted onto the threaded rod and the guide rod. The sleeve fitted onto the threaded rod is threadedly connected to the threaded rod. The servo motor is connected to one end of the threaded rod to provide rotational driving force for the threaded rod.

[0023] The first driving component 303 includes a servo motor and a threaded rod. The threaded rod is rotatably connected in the length adjustment groove 302. The servo motor provides rotational driving force for the threaded rod. The head height adjustment bracket 4 is threadedly connected to the threaded rod.

[0024] A limiting groove is provided at one end of the extension rod where the ear rod 503 is set in a direction perpendicular to the extension rod. The ear rod 503 is placed in the limiting groove. The opposite ends of the two ear rods 503 are provided with ear plugs. The ear rods 503 are fixed and limited by bolts to tighten the gaskets to the end of the extension rod.

[0025] The second driving component 401 includes a threaded rod, a servo motor, and a guide rod. The two guide rods are symmetrically arranged on the head height adjustment frame 4. The threaded rod is arranged parallel to the guide rods and is rotatably connected to the head height adjustment frame 4. The servo motor provides rotational driving force for the threaded rod. The mounting base 5 is connected to both the threaded rod and the two guide rods, and the mounting base 5 and the threaded rod are threadedly connected.

[0026] Specifically, the base plate 101 of the operating table 1 is vertically connected to the vertical plate 102 to form a stable basic support structure. The lifting platform 103 in the sink trough 1011 can be adjusted in height according to the mouse's body size to ensure that the mouse's body is in a suitable position for head fixation. The height adjustment component 2 can drive the fixing plate 3 to move along the height direction, providing a height-adaptive basis for the ear rod 503 to accurately extend into the mouse's ear, avoiding head clamping deviation due to improper ear rod 503 height. The two symmetrically arranged extension strips 301 provide a symmetrical mounting carrier for the ear rod 503, ensuring that the ear rod 503 clamps the mouse's head with balanced force on both sides, preventing head tilting caused by unilateral force. The length adjustment groove 302 cooperates with the first driving component 303 to adjust the horizontal distance between the mouse's head and mouth according to different mice. The craniotomy height adjustment frame 4 position allows the incisor hook 502 to be precisely aligned with the mouse's mouth; the second drive component 401 drives the mounting base 5 to rise and fall, which can adapt to different mouse head heights, ensuring that the incisor hook 502 can be smoothly inserted into the mouse's mouth. At the same time, the fixing hole 501 of the mounting base 5 limits the mouse's lower jaw. With the clamping of the ear bar 503 and the hooking of the incisor hook 502, the mouse's head can be accurately fixed in multiple directions, solving the problem of poor adaptability of traditional head fixation components and reducing experimental errors caused by mouse stress response and unstable fixation; the ear bar 503 and the extension strip 301 are detachably connected, which makes it easy to replace the ear bar 503 of different specifications according to the size of the mouse's ear, further improving the adaptability of the device and reducing the risk of damage to the mouse's ear while ensuring stable clamping.

[0027] In an exemplary embodiment, see Figure 2 A counterweight plate 104 is provided on the side of the base plate 101 that is away from the lifting platform 103.

[0028] Specifically, the counterweight plate 104 is fixed below the base plate 101, which can significantly increase the weight of the bottom of the device, effectively lower the overall center of gravity of the device, and prevent the device from tipping over or shaking due to the combined weight of the fixed plate 3, the skull height adjustment frame 4, the mounting base 5, etc., or the external force applied during the experimental operation. By improving the overall stability of the device, it provides a stable operating basis for precise operations such as skull drilling and drug injection during the microinjection process in the mouse ventricle, reduces operational deviations caused by device instability, ensures the safety of the experimental process, reduces experimental errors, and improves the reliability of experimental data.

[0029] In an exemplary embodiment, see Figure 1 , Figure 2 and Figure 4It also includes an anti-fouling component 6, which includes a release roller 601, a recovery box 602, and a recovery element 603. The release roller 601 is mounted on the counterweight plate 104 and located on one side of the base plate 101. A protective film 604 is wound around the release roller 601. The recovery box 602 is mounted on the counterweight plate 104 and located on the side of the base plate 101 away from the release roller 601. An opening is provided on the side of the recovery box 602 near the base plate 101. The recovery element 603 is located at the opening and is used to recover the protective film 604 released by the release roller 601.

[0030] The 604 protective film can be a waterproof TPU film.

[0031] Specifically, the protective film 604 wound on the release roller 601 can be quickly pulled out and covers the surface of the lifting platform 103, allowing the mouse body to directly contact the protective film 604 instead of the lifting platform 103 itself. This effectively blocks the contaminants generated by the mouse's urination and defecation during the experiment, as well as those formed by drug leakage and tissue fluid overflow, preventing contaminants from directly adhering to the lifting platform 103 and the base plate 101. The recovery box 602 and the release roller 601 are located on opposite sides of the base plate 101, and their openings provide a channel for the recovery of the protective film 604. The recovery unit 603 can efficiently recover the used protective film 604, enabling convenient replacement of the protective film 604 without requiring the experimenter to interrupt the experiment to clean the operating table 1 one by one, greatly improving the cleaning efficiency of the operating table 1. Through the isolation effect of the protective film 604, cross-contamination in subsequent experiments caused by contaminant residues is completely eliminated, ensuring the accuracy of experimental data. At the same time, it avoids damage to the structural stability of the device caused by repeated disassembly and cleaning, meeting the requirements of high-throughput neuroscience experiments for operational continuity.

[0032] In an exemplary embodiment, see Figure 2 and Figure 4 The recycling component 603 includes a recycling roller 6031, a driven gear 6032, and a driving gear 6033. The two recycling rollers 6031 are spaced apart along the height direction of the opening and are rotatably connected to the recycling box 602. The driven gear 6032 is located at one end of the recycling roller 6031. The driving gear 6033 meshes with the driven gear 6032, and a recycling motor 6034 is coaxially connected to the end of the driving gear 6033 that is away from the recycling roller 6031.

[0033] Specifically, the two spaced-apart recovery rollers 6031 provide stable clamping for the protective film 604, ensuring that the film remains flat during recovery and preventing wrinkles that could hinder recovery or cause contaminants to fall onto the operating table 1. The driven gear 6032 is fixedly connected to one end of the recovery rollers 6031, and the driving gear 6033 meshes with the driven gear 6032 and is coaxially driven by the recovery motor 6034. The stable power provided by the motor enables automated recovery of the protective film 604, eliminating the need for manual pulling and reducing the workload of experimenters. It also ensures a uniform recovery speed, preventing uneven manual recovery forces that could cause the film 604 to break or result in incomplete recovery. This automated recovery method further enhances the ease of use and efficiency of the anti-fouling component 6, ensuring that the lifting platform 103 can quickly return to a clean state after each experiment, laying the foundation for rapid subsequent experiments and improving the overall automation level and operational standardization of the device.

[0034] In an exemplary embodiment, see Figure 1 and Figure 6 The multi-functional fixing device also includes a three-coordinate positioning component 7, which includes a Z-axis mounting bracket 701, a Y-axis mounting bracket 702, and an X-axis drive component 705. The Z-axis mounting bracket 701 is located on one side of the base plate 101, and the Z-axis drive component 703 is mounted on the Z-axis mounting bracket 701. The Y-axis mounting bracket 702 is connected to the Z-axis drive component 703 and has a sliding degree of freedom along the Z-axis mounting bracket 701. The Y-axis mounting bracket 702 is mounted on the Y-axis drive component 704. The X-axis drive component 705 is connected to the Y-axis drive component 704.

[0035] The Z-axis drive component 703 includes a threaded rod and a servo motor. The threaded rod is rotatably connected to the Z-axis mounting bracket 701, and the servo motor provides power to drive the threaded rod.

[0036] The Y-axis drive component 704 includes a threaded rod and a servo motor. The threaded rod is rotatably connected to the Y-axis mounting bracket 702, and the servo motor provides power to the threaded rod.

[0037] The X-axis drive unit 705 consists of a servo motor and a ball screw. One end of the ball screw is threadedly connected to the threaded rod of the Y-axis drive unit 704, and the other end is coaxially connected to the servo motor.

[0038] A support rod is connected between the Y-axis mounting bracket 702 and the Z-axis mounting bracket 701. One end of the support rod is threaded to the threaded rod on the Z-axis drive component 703, and the other end is connected to the bottom of the Y-axis mounting bracket 702.

[0039] Specifically, the Z-axis mounting bracket provides a stable base for the Z-axis drive component, which precisely controls the vertical displacement of the Y-axis mounting bracket, meeting the precise positioning requirements of the operating component in the height direction. The Y-axis drive component on the Y-axis mounting bracket enables the smooth movement of the X-axis drive component in the front-back direction, ensuring that the operating component can be accurately aligned with the target position in the front-back direction of the mouse's head. The X-axis drive component provides power for the left-right position adjustment of the operating component, allowing the operating component to cover the left and right areas of the mouse's head. This three-coordinate positioning component 7 replaces the traditional manual handheld operation method, eliminating the need for experimenters to rely on experience to control the operation position. It can achieve precise positioning of the operating component in three-dimensional space through mechanical drive, providing precise positional support for subsequent skull drilling and ventricular injection, avoiding problems such as brain structural damage or inaccurate injection position caused by manual positioning deviation, reducing reliance on experimenter experience, enabling novices to perform precise operations, significantly improving the accuracy, safety, and repeatability of experimental operations, and ensuring the reliability of experimental data.

[0040] In an exemplary embodiment, see Figure 7 It also includes a mounting block 8, a fine-tuning gear 9, a moving block 10, a drill 11, a limiting clamp 12, and a syringe 13; wherein, the mounting block 8 includes a first connecting surface 801 and a second connecting surface 802, the first connecting surface 801 is provided with a receiving groove 803, and guide grooves 804 are symmetrically opened on both sides of the receiving groove 803. The mounting block 8 is connected to the X-axis drive component 705, and the mounting block 8 can move along the length direction of the X-axis drive component 705; the fine-tuning gear 9 is rotatably connected to the receiving groove 802. The groove 803 is provided with a knob 901 at one end; the moving block 10 is provided with a rack 1001 on one side, and guide blocks 1002 are symmetrically provided on both sides of the rack 1001. The guide blocks 1002 are slidably connected in the guide groove 804, and the rack 1001 meshes with the fine-tuning gear 9; the drill 11 is detachably connected to the side of the moving block 10 near the base plate 101; the limiting clamp 12 is provided on the second connecting surface 802; the syringe 13 is detachably connected to the limiting clamp 12 by bolts.

[0041] Specifically, the first connecting surface 801 of the mounting block 8 provides installation and movement space for the fine-tuning gear 9 and the moving block 10 through the receiving groove 803 and the guide groove 804. The mounting block 8 can move along the X-axis drive component, coarsely positioning the operating component in conjunction with the three-coordinate positioning component 7. The fine-tuning gear 9 meshes with the rack 1001 of the moving block 10. The moving block 10 slides in conjunction with the guide groove 804 through the guide block 1002. Rotating the knob 901 drives the moving block 10 to move smoothly, achieving fine adjustment of the height of the drill 11. This compensates for possible positional deviations after coarse adjustment by the three-coordinate positioning component 7, ensuring that the drill 11 accurately abuts the mouse skull marker, precisely controlling the drilling depth, and preventing excessive drilling that could damage the mouse's brain. This design avoids the problem of shallow drilling, which prevents the hole from being opened, thus significantly improving the accuracy of drilling operations. The limiting clamp 12 on the second connecting surface 802 of the mounting block 8 can firmly fix the syringe 13, preventing the syringe 13 from shaking and causing the injection position to shift during injection. The syringe 13 and the limiting clamp 12 are detachably connected, making it convenient to replace syringes 13 of different specifications according to the dosage of the drug, meeting diverse injection needs. This structure integrates the drilling and injection components into the same mounting block 8, eliminating the need to adjust the positions of the two components separately, simplifying the operation process. Furthermore, the dual positioning method of "coarse adjustment (three-coordinate positioning) + fine adjustment (knob 901 drive)" further improves the accuracy of operation, reduces human error, and ensures the smooth progress of drilling and injection operations.

[0042] In an exemplary embodiment, see Figure 3 The lifting platform 103 includes multiple lifting cylinders 1032 and a placement plate 1031. The multiple lifting cylinders 1032 are evenly arranged in the sinking trough 1011, and the placement plate 1031 is located at the top pushing end of the multiple lifting cylinders 1032.

[0043] Specifically, multiple evenly distributed lifting cylinders 1032 can provide uniform and stable support for the placement plate 1031, preventing the placement plate 1031 from tilting due to uneven force, ensuring that the mouse body remains stable when placed on the placement plate 1031, and preventing the mouse's head position from shifting due to the tilt of the placement plate 1031, which would affect the accuracy of subsequent head fixation and experimental operations.

[0044] In some embodiments, this application provides a method for using a multifunctional fixation device for mouse ventricular microinjection. This method is applied to the multifunctional fixation device for mouse ventricular microinjection provided in any of the above embodiments, and includes the following steps: S1: First, control the lifting platform 103 to rise, pull the protective film 604 out from the release roller 601 and stick it to the upper surface of the lifting platform 103, and continue to pull it out until one end of the protective film 604 extends into the opening of the recycling box 602. The recycling roller 6031 drives the protective film 604 into the recycling box 602, thereby tightening the protective film 604. S2: Fix the mouse body on the protective film 604 on the lifting platform 103. Adjust the height of the fixing plate 3 to be at the same height as the mouse ear according to the position of the mouse ear. Then insert the ear rod 503 into the mouse ear. Next, adjust the height of the mounting base 5 according to the position of the mouse mouth so that the mouse jaw is in the fixing hole 501 and the incisor hook 502 is inserted into the mouse mouth to fix the mouse head. S3: The experimenters began to make an opening in the mouse's head, and then marked the location of the opening on the mouse's skull with a marker. S4: The experimenter manipulates the three-coordinate positioning component 7 to move the mounting block 8 to the top of the mouse's head, keeping the drill bit of the drill 11 against the marked position. At this time, the drill is turned on to drill a hole in the mouse's skull. The experimenter operates the knob 901 to control the drill 11 to move downward to complete the opening. Then, the experimenter operates the knob 901 to control the drill 11 to rise to a safe position. Then, the syringe 13 is installed on the limiting clamp 12. The three-coordinate positioning component 7 is adjusted to move the syringe 13 to the opening position. The drug in the syringe 13 is injected into the mouse's ventricle. Then, the three-coordinate positioning component 7 is manipulated to move the mounting block 8 away from the area above the mouse. S5: The experimenter sutures the skin on the mouse's head. After suturing, the mouse is removed from the lifting platform 103. If no obvious contamination is caused on the protective film 604 during the experiment, the recovery roller 6031 will carry the exposed part of the protective film 604 into the recovery box 602. If obvious contamination is caused, the experimenter needs to clean the obvious contaminants on the protective film 604 and then the recovery roller 6031 will carry the contaminated part of the protective film 604 into the recovery box 602.

[0045] The technical features of the above embodiments can be combined in any way (as long as there is no contradiction in the combination of these technical features). For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described; these embodiments not explicitly written should also be considered to be within the scope of this specification.

[0046] The present application has been described in a relatively specific and detailed manner above through general descriptions and specific embodiments. It should be understood that, based on the technical concept of the present application, several conventional adjustments or further innovations can be made to these specific embodiments; however, as long as they do not depart from the technical concept of the present application, the technical solutions obtained by these conventional adjustments or further innovations also fall within the protection scope of the claims of the present application.

Claims

1. A multifunctional fixation device for intracranial microinjection in mice, characterized in that, include: The operating platform (1) includes a vertically arranged base plate (101) and a vertical plate (102). The vertical plate (102) is connected to one side of the base plate (101). A sinkhole (1011) is provided on the top surface of the base plate (101), and a lifting platform (103) is provided in the sinkhole (1011). A height adjustment component (2) is arranged parallel to the vertical plate (102) and installed on the side of the vertical plate (102) near the bottom plate (101); A fixed plate (3) is provided at the moving end of the height adjustment component (2). The height adjustment component (2) provides lifting driving force for the fixed plate (3). Two extension strips (301) are symmetrically provided on the side of the fixed plate (3) away from the height adjustment component (2). The two extension strips (301) are located above the lifting platform (103). A length adjustment groove (302) is provided on the top surface of the fixed plate (3). A first driving member (303) is provided in the length adjustment groove (302). A head height adjustment frame (4) is provided in the length adjustment groove (302) and connected to the first drive member (303). The first drive member (303) provides the head height adjustment frame (4) with a driving force to move closer to and away from the lifting platform (103). A second drive member (401) is provided on the head height adjustment frame (4). Mounting base (5) is connected to the second driving member (401), the second driving member (401) provides lifting driving force for the mounting base (5), and the mounting base (5) has a fixing hole (501) at one end away from the head height adjustment frame (4); The incisor hook (502) is provided on the mounting base (5) and located above the fixing hole (501); Two ear rods (503) are symmetrically arranged at one end of the two extension strips (301) away from the fixing plate (3) and are detachably connected to the extension strips (301).

2. The multifunctional fixation device for intracranial microinjection in mice according to claim 1, characterized in that, The base plate (101) is provided with a counterweight plate (104) on the side facing away from the lifting platform (103).

3. The multifunctional fixation device for intracranial microinjection in mice according to claim 2, characterized in that, The multifunctional fixing device also includes a dirt-proof component (6), which includes: A release roller (601) is disposed on the counterweight plate (104) and located on one side of the base plate (101). A protective film (604) is wound around the release roller (601). The recycling bin (602) is located on the counterweight plate (104) on the side of the base plate (101) away from the release roller (601), and the recycling bin (602) has an opening on the side of the base plate (101); A recovery component (603) is provided at the opening for recovering the protective film (604) released by the release roller (601).

4. The multifunctional fixation device for intracranial microinjection in mice according to claim 3, characterized in that, The recyclable component (603) includes: Two recycling rollers (6031) are spaced apart along the height direction of the opening and are rotatably connected to the recycling bin (602); A driven gear (6032) is disposed at one end of the recovery roller (6031); The drive gear (6033) meshes with the driven gear (6032), and the end of the drive gear (6033) facing away from the recycling roller (6031) is coaxially connected to the recycling motor (6034).

5. The multifunctional fixation device for intracranial microinjection in mice according to claim 1, characterized in that, The multifunctional fixing device further includes a three-coordinate positioning component (7), which includes: A Z-axis mounting bracket (701) is provided on one side of the base plate (101), and a Z-axis drive component (703) is provided on the Z-axis mounting bracket (701); The Y-axis mounting bracket (702) is connected to the Z-axis drive (703) and has a sliding degree of freedom along the Z-axis mounting bracket (701). The Y-axis mounting bracket (702) is provided with a Y-axis drive (704). The X-axis drive (705) is connected to the Y-axis drive (704).

6. The multifunctional fixation device for intracranial microinjection in mice according to claim 5, characterized in that, The multifunctional fixing device also includes: The mounting block (8) includes a first connecting surface (801) and a second connecting surface (802). The first connecting surface (801) is provided with a receiving groove (803). The receiving groove (803) is symmetrically provided with guide grooves (804) on both sides. The mounting block (8) is connected to the X-axis drive component (705). The mounting block (8) can move along the length direction of the X-axis drive component (705). A fine-tuning gear (9) is rotatably connected in the receiving groove (803), and a knob (901) is provided at one end; The movable block (10) has a rack (1001) on one side, and guide blocks (1002) are symmetrically arranged on both sides of the rack (1001). The guide blocks (1002) are slidably connected in the guide groove (804). The rack (1001) meshes with the fine-tuning gear (9). A drill bit (11) is detachably connected to the side of the movable block (10) near the base plate (101); A limiting clamp (12) is provided on the second connecting surface (802); The syringe (13) is detachably connected to the limiting clamp (12).

7. The multifunctional fixation device for intracranial microinjection in mice according to claim 1, characterized in that, The lifting platform (103) includes multiple lifting cylinders (1032) and a placement plate (1031). The multiple lifting cylinders (1032) are evenly arranged in the sinking trough (1011), and the placement plate (1031) is located at the top pushing end of the multiple lifting cylinders (1032).

8. A method of using a multifunctional fixation device for microinjection into the ventricles of mice, characterized in that, The method, applied to the multifunctional fixing device according to any one of claims 1-7, comprises the following steps: S1: First, control the lifting platform (103) to rise, pull the protective film (604) out from the release roller (601) and stick it to the upper surface of the lifting platform (103), and continue to pull it out until one end of the protective film (604) extends into the opening of the recycling box (602). The recycling roller (6031) drives the protective film (604) into the recycling box (602) to tighten the protective film (604). S2: Fix the mouse body on the protective film (604) on the lifting platform (103), adjust the height of the fixing plate (3) according to the position of the mouse's ears so that it is at the same height as the mouse's ears, and then insert the ear rod (503) into the mouse's ears. Next, adjust the height of the mounting base (5) according to the position of the mouse's mouth so that the mouse's lower jaw is in the fixing hole (501), and the incisor hook (502) is inserted into the mouse's mouth to fix the mouse's head. S3: The experimenters began to make an opening in the mouse's head, and then marked the location of the opening on the mouse's skull with a marker. S4: The experimenter manipulates the three-coordinate positioning component (7) to move the mounting block (8) to the top of the mouse's head, keeping the drill bit of the drill (11) against the mark. At this time, the drill is turned on to drill the mouse's skull. The experimenter operates the knob (901) to control the drill (11) to move downward to complete the opening. Then, the experimenter operates the knob (901) to control the drill (11) to rise to a safe position. Then, the syringe (13) is installed on the limit clamp (12). The three-coordinate positioning component (7) is adjusted to move the syringe (13) to the opening position. The medicine in the syringe (13) is injected into the mouse's ventricle. Then, the three-coordinate positioning component (7) is manipulated to move the mounting block (8) away from the area above the mouse. S5: The experimenter sutures the skin on the mouse's head. After suturing, the mouse is removed from the lifting platform (103). If no obvious contamination is caused on the protective film (604) during the experiment, the recovery roller (6031) will carry the protective film (604) to collect the exposed part of the protective film (604) into the recovery box (602). If obvious contamination is caused, the experimenter needs to clean the obvious contaminants on the protective film (604) and then the recovery roller (6031) will collect the contaminated part of the protective film (604) into the recovery box (602).

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