A multi-vitamin soothing and repairing composition containing oxidized trans-resveratrol microcapsules and application thereof

Abstract

The present application relates to a kind of multi-dimensional soothing care composition containing oxyresveratrol microcapsule and application, the composition includes the following components: oxyresveratrol microcapsule, kava leaf / root / stem extract, Tokyo sakura leaf extract and flaxseed extract;Oxyresveratrol microcapsule in the composition of the present application, using inulin as wall material, oxyresveratrol is wrapped, increase its solubility in water, and inulin can also improve the moisturizing effect of composition.The composition of the present application can achieve the effect of soothing and repairing from multiple aspects through the synergy of the four ingredients of oxyresveratrol microcapsule, kava leaf / root / stem extract, Tokyo sakura leaf extract and flaxseed extract.

Description

A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules and its application Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules and its application. Background Technology

[0002] Oxidized resveratrol is a natural polyphenol compound that is widely found in plants such as mulberry and jackfruit. It has significant antioxidant, anti-inflammatory, neuroprotective, tyrosinase inhibition, and anti-aging biological activities, showing broad application prospects in the fields of cosmetics, health products, and pharmaceuticals.

[0003] However, oxidized resveratrol has extremely poor water solubility, with its solubility in water being far lower than its solubility in organic solvents (such as methanol and ethanol). This severely limits its application in water-based formulations. Previous studies have employed methods such as using organic solvents to aid solubility, preparing liposomes, and using cyclodextrin inclusion complexes to improve its water solubility. However, these methods suffer from problems such as complex processes, high costs, the risk of organic solvent residue, and poor stability, making it difficult to meet the safety requirements of industrial production and consumers.

[0004] Therefore, it is of great significance to develop an efficient, safe, and industrially applicable method to improve the water solubility of oxidized resveratrol and, on this basis, construct an active composition with synergistic effects. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides a multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, the composition comprising the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; wherein the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is (0.5-2):(0.05-0.5):(0.05-0.3):(0.1-1);

[0008] The preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0009] S1. Add inulin to 8-10 times the volume of an acetate-sodium acetate buffer solution with a pH of 3-4. After it is completely dissolved, slowly add resveratrol oxide. The ratio of resveratrol oxide to inulin is 1:8-10. Continue stirring until a homogeneous mixed solution is formed. The mass ratio of resveratrol oxide to inulin is 1:(8-10).

[0010] S2. The mixed solution is subjected to ultrasonic treatment and then freeze-dried to obtain inulin-encapsulated resveratrol oxide, which is the resveratrol oxide microcapsule.

[0011] The specific functions of the four components in the composition of this invention are as follows:

[0012] Oxidized resveratrol microcapsules utilize inulin to encapsulate oxidized resveratrol. Inulin is a plant oligosaccharide with good water solubility, which enhances the water solubility of the microcapsules. Additionally, inulin increases the water content of the stratum corneum and exhibits good inhibitory effects against bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus, effectively maintaining the skin's microecological balance. Oxidized resveratrol can reduce the inflammatory response of keratinocytes by inhibiting the nuclear factor kappa B (NF-κB) signaling pathway.

[0013] Capsaicin in the leaf / root / stem extract of kava pepper can regulate neurotransmitters and enhance the inhibition of nerve pain transmission by affecting the binding of GABA to GABAA receptors, thus playing a sedative role.

[0014] The cherry blossom leaf extract from Tokyo contains components such as cherry blossom acid and cherry blossom alkaloids, which can promote the formation of the stratum corneum, repair damaged skin, and promote the regeneration of collagen in the skin. In addition, components such as isoflavones and cherry flavonoids also have soothing and anti-inflammatory effects.

[0015] Flaxseed extract has an antagonistic effect on NK-1 receptors, which can prevent vasodilation and mast cell degranulation caused by substance P, thereby reducing skin redness and sensitivity. In addition, the Omega-3 fatty acids contained in flaxseed extract can penetrate deep into the skin to replenish moisture and enhance the skin's ability to retain moisture.

[0016] Preferably, the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract and flaxseed extract is (0.8-1.5):(0.1-0.3):(0.05-0.15):(0.3-0.8).

[0017] Most preferably, the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 1:0.2:0.1:0.5.

[0018] Preferably, the method for preparing the acetate-sodium acetate buffer solution includes the following steps: taking 200 mL of 0.1 mol / L sodium acetate solution and placing it in a 500 mL beaker, adding 0.1 mol / L acetic acid solution dropwise with a pipette until the pH is 3-4, thereby obtaining the acetate-sodium acetate buffer solution, which is then sealed and stored for later use.

[0019] Preferably, in step S2 of the preparation of the oxidized resveratrol microcapsules, the ultrasonic treatment power is 200-300W, the temperature is 20-35℃, the ultrasonic treatment is repeated 3-4 times, and each time is 1-2 min; the freeze-drying temperature is (-50)-(-65)℃, and the time is 24-72h.

[0020] In a second aspect, the present invention provides the application of the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules in the first aspect in the preparation of cosmetics.

[0021] Preferably, the cosmetic is one of toner, lotion, cream, mask and serum, and the amount of the soothing and repairing composition added is 1%-5% of the total weight of the cosmetic.

[0022] Thirdly, the present invention provides a soothing and repairing lotion comprising the following ingredients by weight percentage: 1%-5% of the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in the first aspect, 2%-4% of moisturizer, 0.1%-0.3% of thickener, 2%-4% of emulsifier, 5%-8% of oil, 0.5%-4% of preservative, 0.01%-0.3% of pH adjuster, and the balance being deionized water.

[0023] Preferably, the moisturizer is at least one selected from allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerol, budding stalk polysaccharide, and ceramide.

[0024] Preferably, the thickener is at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum.

[0025] Preferably, the emulsifier is at least one selected from cetearyl alcohol, polyglycerol-6 distearate, and C14-22 alkyl alcohol / C12-20 alkyl glucoside.

[0026] Preferably, the oil is at least one of coconut oil alcohol-octanoate / decanoate, polydimethylsiloxane, hydrogenated palm kernel oil, and polymethylsilsesquioxane.

[0027] Preferably, the preservative is at least one selected from 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0028] Preferably, the pH adjuster is arginine and / or tromethamine.

[0029] Fourthly, the present invention provides a method for preparing the soothing and repairing lotion of the third aspect, comprising the following steps:

[0030] (1) Mix the humectant, thickener and part of deionized water, and homogenize at 75-85℃ to obtain an aqueous phase;

[0031] (2) Homogenize the emulsifier and oil at 75-85℃ to obtain the oil phase;

[0032] (3) Stir the aqueous phase and oil phase evenly, and add the preservative after cooling to 55-65℃. Stir evenly, and after the temperature drops to 35-45℃, first add the remaining deionized water and oxidized resveratrol microcapsules and stir to obtain a solution. Then add the remaining components in the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules and stir evenly. Finally, add pH adjuster to adjust the pH to obtain the soothing and repairing emulsion.

[0033] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0034] The oxidized resveratrol microcapsules in the composition of this invention use inulin as a wall material to encapsulate the oxidized resveratrol, increasing its solubility in water. Furthermore, inulin also enhances the moisturizing effect of the composition. Through the synergistic effect of four components—oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract—the composition of this invention achieves soothing and repairing effects on multiple levels. Attached Figure Description

[0035] Figure 1 shows the facial redness of the subjects before and after using the product described in Application Example 1. Detailed Implementation

[0036] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0037] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0038] Oxyresveratrol: Manufacturer: Naturalis Srl; Trade name: OXYRESVERATROL;

[0039] Kava Pepper Leaf / Root / Stem Extract: Manufacturer is Sethic, France; trade name is Kava Extract.

[0040] Tokyo cherry blossom leaf extract: manufactured by Japan's Ichimaru Natural Beauty Co., Ltd., product name SAKURAExtract B;

[0041] Flaxseed extract: The manufacturer is Shaanxi Ruimao Biotechnology Co., Ltd., and the product name is flaxseed extract.

[0042] Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0043] Example 1

[0044] A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, the composition comprising the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 1:0.2:0.1:0.5; the total mass of the composition is 100 parts.

[0045] The preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0046] S1. Add inulin to 9 times its volume of an acetate-sodium acetate buffer solution with a pH of 3.5. After complete dissolution, slowly add resveratrol oxide, with a resveratrol oxide to inulin ratio of 1:9. Continue stirring until a homogeneous mixed solution is formed. The mass ratio of resveratrol oxide to inulin is 1:9, and the stirring speed is 500 rpm. The preparation method of the acetate-sodium acetate buffer solution includes the following steps: Take 200 mL of 0.1 mol / L sodium acetate solution and place it in a 500 mL beaker. Add 0.1 mol / L acetic acid solution dropwise using a pipette, while monitoring the pH value of the solution in real time with a pH meter until the pH reaches 3.5 to obtain the acetate-sodium acetate buffer solution. Seal and store for later use.

[0047] S2. The mixed solution is subjected to ultrasonic treatment and then freeze-dried to obtain inulin-encapsulated oxidized resveratrol, which is the oxidized resveratrol microcapsule; wherein, the ultrasonic treatment power is 250W, the temperature is 25℃, the ultrasonic treatment is repeated 3 times, and each time is 2min; the freeze-drying temperature is -55℃ and the time is 36h.

[0048] Example 2

[0049] A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, the composition comprising the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 0.8:0.3:0.15:0.3; the total mass of the composition is 100 parts. The preparation method of the oxidized resveratrol microcapsules is the same as in Example 1.

[0050] Example 3

[0051] A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, the composition comprising the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 1.5:0.1:0.05:0.8; the total mass of the composition is 100 parts. The preparation method of the oxidized resveratrol microcapsules is the same as in Example 1.

[0052] Example 4

[0053] A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules is disclosed. The composition comprises the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 0.5:0.5:0.3:1; the total mass of the composition is 100 parts. The preparation method of the oxidized resveratrol microcapsules is the same as in Example 1.

[0054] Example 5

[0055] A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, the composition comprising the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 5:0.05:0.05:0.1; the total mass of the composition is 100 parts. The preparation method of the oxidized resveratrol microcapsules is the same as in Example 1.

[0056] Example 6

[0057] The only difference between Example 6 and Example 1 is that the preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0058] The preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0059] S1. Add inulin to 10 times its volume of an acetate-sodium acetate buffer solution with a pH of 4. After complete dissolution, slowly add resveratrol oxide and continue stirring until a homogeneous mixture is formed. The mass ratio of resveratrol oxide to inulin is 1:10, and the stirring speed is 600 rpm. The preparation method of the acetate-sodium acetate buffer solution includes the following steps: Take 200 mL of 0.1 mol / L sodium acetate solution and place it in a 500 mL beaker. Add 0.1 mol / L acetic acid solution dropwise using a pipette, while monitoring the pH value of the solution in real time with a pH meter until the pH reaches 4. The acetate-sodium acetate buffer solution is then obtained and sealed for later use.

[0060] S2. The mixed solution is subjected to ultrasonic treatment and then freeze-dried to obtain inulin-encapsulated oxidized resveratrol, which is the oxidized resveratrol microcapsule; wherein, the ultrasonic treatment power is 200W, the temperature is 20℃, the ultrasonic treatment is repeated 4 times, and each time is 1min; the freeze-drying temperature is -55℃ and the time is 24h.

[0061] Example 7

[0062] The only difference between Example 7 and Example 1 is that the preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0063] The preparation method of the oxidized resveratrol microcapsules includes the following steps:

[0064] S1. Add inulin to 8 times its volume of an acetate-sodium acetate buffer solution with a pH of 3. After complete dissolution, slowly add resveratrol oxide and continue stirring until a homogeneous mixture is formed. The mass ratio of resveratrol oxide to inulin is 1:8, and the stirring speed is 400 rpm. The preparation method of the acetate-sodium acetate buffer solution includes the following steps: Take 200 mL of 0.1 mol / L sodium acetate solution and place it in a 500 mL beaker. Add 0.1 mol / L acetic acid solution dropwise using a pipette, while monitoring the pH value of the solution in real time with a pH meter until the pH reaches 3. The acetate-sodium acetate buffer solution is then obtained and sealed for later use.

[0065] S2. The mixed solution is subjected to ultrasonic treatment and then freeze-dried to obtain inulin-encapsulated oxidized resveratrol, which is the oxidized resveratrol microcapsule; wherein, the ultrasonic treatment power is 300W, the temperature is 35℃, the ultrasonic treatment is repeated 3 times, and each time is 2min; the freeze-drying temperature is -50℃ and the time is 72h.

[0066] Comparative Example 1

[0067] The difference between Comparative Example 1 and Example 1 is that an equal amount of oxidized resveratrol was used instead of oxidized resveratrol microcapsules.

[0068] Comparative Example 2

[0069] The difference between Comparative Example 2 and Example 1 is that the total weight of the composition remains unchanged, oxidized resveratrol microcapsules are not added, and the missing amount is made up by kava leaf / root / stem extract, Tokyo cherry blossom leaf extract and flaxseed extract in a mass ratio of 0.2:0.1:0.5.

[0070] Comparative Example 3

[0071] The difference between Comparative Example 3 and Example 1 is that the total weight of the composition remains unchanged, no kava leaf / root / stem extract is added, and the missing amount is made up by oxidized resveratrol microcapsules, Tokyo cherry blossom leaf extract and flaxseed extract in a mass ratio of 1:0.1:0.5.

[0072] Comparative Example 4

[0073] The difference between Comparative Example 4 and Example 1 is that the total weight of the composition remains unchanged, Tokyo cherry blossom leaf extract is not added, and the missing amount is made up by oxidized resveratrol microcapsules, kava leaf / root / stem extract and flaxseed extract in a mass ratio of 1:0.2:0.5.

[0074] Comparative Example 5

[0075] The difference between Comparative Example 5 and Example 1 is that the total weight of the composition remains unchanged, flaxseed extract is not added, and oxidized resveratrol microcapsules, kava leaf / root / stem extract and Tokyo cherry blossom leaf extract in a mass ratio of 1:0.2:0.1 are used to make up for the missing amount.

[0076] Test Example 1: Water Solubility Test of Microcapsules

[0077] The method for testing the solubility of oxidized resveratrol microcapsules / oxidized resveratrol is as follows:

[0078] S1. Add 0.5g of oxidized resveratrol microcapsules obtained in Example 1 and 0.1g of oxidized resveratrol in Comparative Example 1 to 10mL of water respectively, and stir continuously at 20℃ for 48h until they are dissolved evenly to obtain two mixed solutions.

[0079] S2. Filter both mixed solutions using a 0.22μm membrane filter, dilute with methanol 1000 times, and measure the content of oxidized resveratrol in the solution. Each sample was measured in triplicate. The specific measurement method is as follows:

[0080] The content X of oxidized resveratrol was determined by HPLC. The test method was as follows: Standard working solution preparation: Oxidized resveratrol standards were prepared with methanol to obtain solutions with concentrations of 0.1 µg / mL, 0.5 µg / mL, 1 µg / mL, 2 µg / mL, 5 µg / mL, and 10 µg / mL. Sample testing method: The filtrate was filtered through a 0.2 µm filter membrane before being used in the HPLC. Test conditions were as follows: C18-150 mm × 3 mm × 2.7 µm column, column temperature 35℃, flow rate 0.8 mL / min, DAD detector, detection wavelength 328 nm, injection volume 5 µL, and mobile phase as shown in Table 1.

[0081] Table 1 Data of the mobile phase

[0082] Time / min A (0.1% phosphoric acid) / % B (methanol) / % 0 10 90 5 10 90 14 80 20 15 10 90 20 10 90 surface

[0083] Calculate using the following formula:

[0084] ;

[0085] Where X is the content of the analyte, %; C is the concentration of the analyte solution obtained according to the standard curve, µg / mL; V is the final volume of the solution, mL; f is the dilution factor; and m is the sample weight, g.

[0086] Solubility data are shown in Table 2.

[0087] Table 2. Solubility data of resveratrol oxidized microcapsules / resveratrol oxidized

[0088] Sample / Performance Solubility (mg / mL): Resveratrol Oxide 0.41; Resveratrol Oxide Microcapsules 33.62 surface

[0089] As shown in Table 2, the present invention encapsulates oxidized resveratrol within inulin to form microcapsules, which can significantly improve the solubility of oxidized resveratrol microcapsules in water, making the composition of the present invention suitable for water-based formulations.

[0090] Test Example 2: Anti-inflammatory effect test of the composition

[0091] TNF-α is the most common inflammatory cytokine in inflammatory responses. It can induce an inflammatory phenotype in vascular endothelial cells, leading to changes in metabolism and hemodynamics, and promoting the production of inflammatory mediators. These inflammatory mediators can increase vascular permeability, causing local congestion and edema, and may also stimulate nerve endings, resulting in skin itching and pain. Therefore, inhibiting TNF-α can help soothe and repair the skin.

[0092] The specific testing method is as follows:

[0093] S1. Plating: Logarithmically growing RAW264.7 cells were seeded into 24-well plates at a cell density of 2 × 10⁻⁶ cells / well. 5 Cells / mL, 0.5mL per well, after plating the plate, mark the top cover of the cell plate and incubate in a CO2 incubator for 24 h;

[0094] S2. Sample processing: After cell incubation for 24 hours, the supernatant was aspirated, and samples of less than 0.5 mL were added respectively: ① Blank control group: DMEM medium; ② Model group: 1 μg / mL LPS (DMEM medium containing 1 μg / mL LPS); ③ Positive control group: 0.001% dexamethasone + 1 μg / mL LPS (DMEM medium containing 0.001 wt% dexamethasone and 1 μg / mL LPS); ④ Experimental groups 1-12: 0.5% composition + 1 μg / mL LPS (DMEM medium containing 0.5 wt% of the compositions of Examples 1-7 and Comparative Examples 1-5 and 1 μg / mL LPS respectively).

[0095] S3. Detection of inflammatory factors in cell supernatants of each group: After 24 hours of cell treatment, the supernatant was pipetted into centrifuge tubes, centrifuged at 1000 rpm for 5 minutes, the precipitate was discarded, and the supernatant was collected. The changes in inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α inflammatory factor detection kit. The results were expressed as TNF-α inhibition rate, calculated as: TNF-α inhibition rate % = (TNF-α level in model group - TNF-α level in experimental group / TNF-α level in blank control group) / TNF-α level in model group × 100%. Specific data are shown in Table 3.

[0096] Table 3. TNF-α inhibition rate data for each composition.

[0097] Group / Performance TNF-α Inhibition Rate / % Example 1 45.72 Example 2 43.93 Example 3 44.51 Example 4 40.86 Example 5 42.26 Example 6 45.20 Example 7 44.84 Comparative Example 1 28.30 Comparative Example 2 24.23 Comparative Example 3 26.36 Comparative Example 4 31.18 Comparative Example 5 34.36 Positive Control Group 47.81 surface

[0098] A higher TNF-α inhibition rate indicates a better anti-inflammatory effect of the composition.

[0099] As shown in Table 3, and in conjunction with the data from Examples 1 and 2-5, when the total mass fraction of the soothing and repairing composition is the same, the anti-inflammatory effect of the composition is at a better level when the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is in the range of (0.8-1.5):(0.1-0.3):(0.05-0.15):(0.3-0.8).

[0100] Based on the data from Example 1 and Comparative Examples 2-5, it can be seen that when one of the following is missing: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract, the TNF-α inhibition rate of the composition is significantly reduced. This indicates that these four ingredients have a good soothing and repairing effect on the skin.

[0101] Based on the data from Example 1 and Comparative Example 1, it can be seen that the TNF-α inhibition rate of Comparative Example 1 is also significantly lower than that of Example 1. This may be because the oxidized resveratrol in Comparative Example 1 was not encapsulated with inulin, and the water solubility of oxidized resveratrol is poor, which prevents oxidized resveratrol from exerting its anti-inflammatory effect.

[0102] Application Example 1-7 and Comparative Application Example 1-5

[0103] The compositions of Examples 1-7 and Comparative Examples 1-5 were added to the soothing and repairing lotion at a concentration of 3 wt% to obtain the soothing and repairing lotions of Application Examples 1-7 and Comparative Application Examples 1-5. The formulations are shown in Table 4.

[0104] The preparation methods of the oil-controlling and acne-reducing lotions used in Examples 1-7 and Comparative Examples 1-5 include the following steps:

[0105] (1) Mix the humectant, thickener and half of the deionized water, and homogenize at 80°C and 1200 rpm for 5 min to obtain the aqueous phase;

[0106] (2) Homogenize the emulsifier and oil at 80°C and 1200 rpm for 5 min to obtain the oil phase;

[0107] (3) Stir the aqueous phase and oil phase evenly at 80°C. After cooling to 60°C, add the preservative and stir evenly at 300 rpm. After the temperature drops to 40°C, first add the remaining deionized water and oxidized resveratrol microcapsules and stir to obtain a solution. Then add the remaining components in the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules and continue stirring for 5 minutes. Finally, add a pH adjuster to adjust the pH to obtain the soothing and repairing emulsion.

[0108] Table 4. Formulations of the soothing and repairing lotions in Application Examples 1-7 and Comparative Application Examples 1-5

[0109]

[0110] Comparative Application Example 6

[0111] Compared to Application Example 6, the emulsion did not contain the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules. Instead, an equal amount of deionized water was used to replace the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules. The preparation method was the same as in Application Example 1.

[0112] Test Example 3: Test on the soothing, repairing, and moisturizing effects of each group of lotions on human skin.

[0113] (1) Experimental basis

[0114] The human efficacy evaluation test method shall be followed in accordance with the "Cosmetic Safety Technical Specifications" (2015 edition).

[0115] (2) Subject screening

[0116] Inclusion criteria: We are recruiting female volunteers aged 18-45 from Asia who meet the following criteria:

[0117] a) Screening healthy volunteers with facial blemishes or dull facial skin;

[0118] b) A self-reported history of skin allergies (confirmed by a dermatologist);

[0119] c) No history of serious systemic diseases or skin diseases.

[0120] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0121] Number of participants: A total of 65 qualified volunteers were included and divided into 13 groups of 5 people each using a random number table.

[0122] (3) Sample application method

[0123] Test samples: Soothing and repairing lotions prepared in Application Examples 1-7 and Comparative Application Examples 1-6 (double-blind method numbering). Among them, the lotion prepared in Comparative Application Example 6 served as the blank control group, and the other application examples and comparative application examples served as the sample groups.

[0124] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0125] (4) Testing cycle and process

[0126] Test period: 14 or 28 days (D0, D) 14 D 28 ).

[0127] Visit time points: D0 (baseline period), D 14 D 28 (At the end of the test) Instrument testing will be conducted.

[0128] Preparation before testing:

[0129] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces.

[0130] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0131] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0132] (5) Test indicators

[0133] The Tewameter®™ Hex (Courage + Khazaka) transdermal moisture loss probe and the Corneometer® CM 825 (Courage + Khazaka) moisture probe were used to test the initial TEWL value and moisture content of the subjects after facial cleansing. The Visia-7 was used to take facial pictures, and the exported red area images were analyzed to obtain the initial a* value.

[0134] The formulas for calculating the evaluation parameters are as follows:

[0135] (T14, 28) TEWL value improvement rate = (TEWL value) T0 -TEWL value Tn ) / TEWL value T0 ×100%; where T n =T 14 T 28 A higher TEWL value improvement rate indicates that the skincare product performs better in enhancing the skin barrier function, improving moisturizing and water-locking ability, and repairing the damaged barrier.

[0136] (T14, 28) Moisture content improvement rate = (Moisture content Tn - Moisture content T0 Moisture content T0 ×100%; where T n =T 14 T 28 The higher the improvement rate in skin hydration, the better the skincare product performs in increasing skin moisture content, enhancing moisturizing ability, and repairing the skin barrier function.

[0137] (T14, 28) a* value improvement rate = (a* value) T0 -a* value Tn ) / a* value T0×100%; where T n =T 14 T 28 A higher improvement rate in skin color a* value indicates better performance of the skincare product in reducing skin redness and improving redness. The results are averaged; see Table 5 for details.

[0138] Table 5. Results of human efficacy tests for each group of emulsions.

[0139]

[0140] As shown in Table 5, compared with Comparative Application Example 6 (blank control group), the moisturizing, repairing and redness-reducing effects of the emulsions in Application Examples 1-7 and Comparative Application Examples 1-5 were improved, indicating that the addition of the soothing and repairing composition is an important parameter affecting moisturizing, repairing and redness-reducing effects.

[0141] Based on the data from Application Examples 1 and 2-5, it can be seen that when the total mass fraction of the soothing and repairing composition is the same, the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is in the range of (0.8-1.5):(0.1-0.3):(0.05-0.15):(0.3-0.8), the soothing, repairing, and moisturizing effects of the composition are at a better level.

[0142] Combining the data from Application Example 1 and Comparative Application Examples 2-5, it can be seen that when one of the following is missing: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract, the soothing, repairing, and moisturizing effects of the lotion are reduced. This is positively correlated with the anti-inflammatory effect of the composition, indicating that these four ingredients work synergistically to have a good soothing and repairing effect on the skin.

[0143] In summary, the composition of the present invention achieves soothing and repairing effects on multiple levels through the synergistic effect of four components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract.

[0144] Based on the data from Application Example 1 and Comparative Application Example 1, it can be seen that the soothing, repairing, and moisturizing performance of the lotion in Comparative Application Example 1 is significantly lower than that in Application Example 1. This may be because the oxidized resveratrol in the lotion of Comparative Application Example 1 is not encapsulated by inulin, which means that the oxidized resveratrol cannot be completely dissolved in the water-based lotion system. As a result, the oxidized resveratrol cannot exert its anti-inflammatory and soothing effects, thereby reducing the soothing and repairing effects of the lotion.

[0145] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, characterized in that, The composition comprises the following components: oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract; the mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is (0.5-2):(0.05-0.5):(0.05-0.3):(0.1-1); wherein, the preparation method of the oxidized resveratrol microcapsules includes the following steps: S1, adding inulin to 8-10 times the amount of an acetate-sodium acetate buffer solution with a pH of 3-4, and slowly adding oxidized resveratrol after complete dissolution, and continuing to stir until a uniform mixed solution is formed; wherein, the mass ratio of oxidized resveratrol to inulin is 1:(8-10); S2, sonicating the mixed solution, and then freeze-drying it to obtain inulin-encapsulated oxidized resveratrol, which is the oxidized resveratrol microcapsules.

2. The multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in claim 1, characterized in that, The mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is (0.8-1.5):(0.1-0.3):(0.05-0.15):(0.3-0.8).

3. The multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in claim 1, characterized in that, The mass ratio of the oxidized resveratrol microcapsules, kava leaf / root / stem extract, Tokyo cherry blossom leaf extract, and flaxseed extract is 1:0.2:0.1:0.

5.

4. The multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in claim 1, characterized in that, In step S2 of the preparation of the oxidized resveratrol microcapsules, the ultrasonic treatment power is 200-300W, the temperature is 20-35℃, the ultrasonic treatment is repeated 3-4 times, and each time is 1-2 min; the freeze-drying temperature is (-50)-(-65)℃, and the time is 24-72h.

5. The use of the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules according to any one of claims 1-4 in the preparation of cosmetics.

6. The application of the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in claim 5 in the preparation of cosmetics, characterized in that, The cosmetic is one of toner, lotion, cream, mask and serum, and the amount of the soothing and repairing composition added is 1%-5% of the total weight of the cosmetic.

7. A soothing and repairing lotion, characterized in that, The ingredients comprise the following ingredients by weight percentage: 1%-5% of the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules as described in any one of claims 1-4, 2%-4% of moisturizer, 0.1%-0.3% of thickener, 2%-4% of emulsifier, 5%-8% of oil, 0.5%-4% of preservative, 0.01%-0.3% of pH adjuster, with the balance being deionized water.

8. The soothing and repairing lotion as described in claim 7, characterized in that, The raw materials are selected from at least one of (a)-(f): (a) the humectant is at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, D-panthenol, sodium hyaluronate, 1,2-butanediol, glycerin, budding stalk polysaccharide, and ceramide; (b) the thickener is at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, and sclerotium gum; (c) the emulsifier is cetearyl alcohol, polyglycerol-6 (d) The oil is at least one of distearate and C14-22 alkyl alcohol / C12-20 alkyl glucoside; (e) The preservative is at least one of coconut oil octanoate / decanoate, polydimethylsiloxane, hydrogenated palm kernel oil and polymethylsilsesquioxane; (f) The pH adjuster is arginine and / or tromethamine.

9. The method for preparing the soothing and repairing lotion according to claim 8, characterized in that, The process includes the following steps: (1) mixing moisturizer, thickener and part of deionized water, homogenizing at 75-85℃ to obtain an aqueous phase; (2) homogenizing emulsifier and oil at 75-85℃ to obtain an oil phase; (3) stirring the aqueous phase and oil phase evenly, adding preservative after cooling to 55-65℃, stirring evenly, adding the remaining deionized water and oxidized resveratrol microcapsules to obtain a solution, then adding the remaining components in the multidimensional soothing and repairing composition containing oxidized resveratrol microcapsules, stirring evenly, and finally adding a pH adjuster to adjust the pH to obtain the soothing and repairing emulsion.

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