A device for detecting avian influenza virus antibody and a detection process thereof

Abstract

The application relates to the technical field of biological detection, in particular to a device for detecting avian influenza virus antibodies, which comprises an instrument main body, a detection cavity is arranged in the instrument main body, three excitation light sources are arranged at the bottom of the detection cavity, a moving rod is slidably arranged on the instrument main body, a circular cavity is arranged in the instrument main body and communicates with the detection cavity, a rotating hole that communicates with the circular cavity is arranged at the upper end of the instrument main body, a rotating disc is rotatably arranged in the circular cavity, a rotating column is vertically rotatably arranged in the rotating hole, a plurality of fixing holes are equidistantly arranged on the circumference of the rotating disc, a plurality of narrow-band optical filters are fixedly arranged in the fixing holes, and the narrow-band optical filters can be sequentially moved into the detection cavity. When multiple antibodies in the avian influenza virus need to be detected, the multiple excitation light sources and the multiple narrow-band optical filters are switched to realize the detection of the multiple antibodies in the avian influenza virus, and the detection efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to a device and detection process for detecting avian influenza virus antibodies. Background Technology

[0002] Avian influenza is an acute infectious disease caused by avian influenza viruses. It is characterized by rapid transmission and high pathogenicity, causing huge economic losses to the poultry farming industry. At the same time, some subtypes of avian influenza viruses can infect humans, seriously threatening human health. Therefore, rapid and accurate detection of avian influenza virus antibodies is crucial for epidemic prevention and control. At present, there are many methods for detecting avian influenza virus antibodies. Among them, immunofluorescence technology is widely used in multi-antibody joint detection scenarios due to its advantages such as high sensitivity, strong specificity, and fast detection speed. Its core principle is to use the specific binding of fluorescent labels with avian influenza virus antibodies, and use an optical system to excite and capture the fluorescence signal to achieve qualitative and quantitative analysis of antibodies.

[0003] During the detection process, in order to avoid interference from background light sources and astigmatism on the detection results, a narrow-band filter is usually installed between the sample and the image sensor to filter out stray light of non-target wavelengths, allowing only the specific wavelength signal light generated by the sample to reach the image sensor, thereby improving the signal-to-noise ratio.

[0004] In the existing technology, since one excitation light source can only excite one fluorescent dye, most devices can only detect a single antibody. If multiple antibodies need to be detected, the excitation light source and narrow-band filter in the detection device need to be replaced, which is cumbersome and has low detection efficiency. Summary of the Invention

[0005] The purpose of this invention is to address the following shortcomings in the prior art: most devices can only detect a single antibody, and if multiple antibodies are to be detected, the excitation light source and narrow-band filter in the detection device need to be replaced, which is cumbersome and has low detection efficiency. Therefore, this invention proposes a device and detection process for detecting avian influenza virus antibodies.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A device for detecting avian influenza virus antibodies includes an instrument body, a detection cavity inside the instrument body, a mounting hole communicating with the detection cavity at the upper end of the instrument body, an image sensor fixedly installed in the mounting hole, a rectangular insertion hole communicating with the detection cavity in the instrument body, a sample placement plate slidably inserted in the rectangular insertion hole, and one end of the sample placement plate extending into the detection cavity. Three different types of excitation light sources are slidably installed at the bottom of the detection chamber via a sliding assembly. The main body of the instrument has a movable hole that communicates with the detection chamber. A movable rod is slidably inserted into the movable hole, and one end of the movable rod is fixedly connected to the side of one of the excitation light sources. The instrument body has a circular cavity communicating with the detection chamber. The upper end of the instrument body has a rotating hole communicating with the circular cavity. A rotating disk is rotatably installed inside the circular cavity. A rotating column is vertically rotatably installed inside the rotating hole. The lower end of the rotating column is fixedly connected to the center of the rotating disk. An adjusting block is fixedly installed at the upper end of the rotating column through the rotating hole. Multiple fixing holes are equidistantly opened on the circumference of the rotating disk. Narrow-band filters are fixedly installed in each of the multiple fixing holes. The multiple narrow-band filters can be moved sequentially into the detection chamber.

[0007] As a preferred embodiment, the sliding assembly includes a slide rail fixedly installed at the bottom of the detection chamber and a sliding seat slidably installed on the slide rail. The three excitation light sources are fixedly installed at equal intervals on the sliding seat. A focusing cover is fixedly installed inside the detection chamber, and the focusing cover is installed between the sample placement plate and the excitation light source.

[0008] As a preferred embodiment, the number of fixing holes is six, and three pairs of positioning grooves are equidistantly opened on the side of the rotating disk. The multiple positioning grooves correspond to multiple fixing holes. The positioning grooves are composed of rectangular grooves and triangular grooves. A sliding hole communicating with the circular cavity is opened on the side of the instrument body. A top rod is horizontally slidably installed in the sliding hole. A triangular positioning block is fixedly installed at one end of the top rod near the rotating disk. The triangular positioning block is inserted into one of the triangular grooves.

[0009] As a preferred embodiment, a bracket is fixedly installed on the side of the instrument body. The bracket has a threaded hole, and an adjusting screw is threaded into the threaded hole. One end of the adjusting screw is rotatably installed on the instrument body, and a rotating block is fixedly installed on the other end. A moving block is threaded onto the adjusting screw, and the lower end of the moving block is fixedly connected to the top rod.

[0010] As a preferred embodiment, the depth of the three pairs of positioning grooves increases sequentially along the rotation direction of the rotating disk, and the instrument body is provided with a transmission component for driving the moving rod to move on its side.

[0011] As a preferred embodiment, a support rod is fixedly installed on the side of the instrument body, and a lever is rotatably installed at one end of the support rod. The rotatable connection between the support rod and the lever is located in the upper half of the lever. An upper slotted hole is opened at the upper end of the lever, and a lower slotted hole is opened at the lower end of the lever. The end of the top rod away from the triangular positioning block is slidably hinged in the upper slotted hole, and one end of the moving rod is slidably hinged in the lower slotted hole of the lever. A protective shell is installed on the side of the instrument body, and the adjusting screw, the moving block, and the lever are all located inside the protective shell.

[0012] As a preferred embodiment, the sample placement plate has stepped placement holes at the portion located within the detection chamber, and a reaction chip is placed within the placement holes. A U-shaped mounting groove is formed on the side of the placement holes, and a sealing airbag is fixedly installed within the mounting groove. A cylindrical hole is formed at one end of the sample placement plate near the detection chamber, and one end of the cylindrical hole communicates with the interior of the sealing airbag. An inflation component for inflating the sealing airbag is provided within the detection chamber, and a locking component for locking the sample placement plate is installed on the front side of the instrument body.

[0013] As a preferred embodiment, the inflation assembly includes a mounting rod horizontally fixedly installed in the detection chamber and a piston fixedly installed at one end of the mounting rod. The piston and the cylindrical hole are located on the same horizontal line, and the diameter of the piston is the same as the diameter of the cylindrical hole.

[0014] As a preferred embodiment, the locking assembly includes a mounting block fixedly mounted on the instrument body, two spring rods symmetrically fixedly mounted on the mounting block, and a limiting block with a right-angled trapezoidal longitudinal section fixedly mounted on one end of the two spring rods, wherein the inclined surface of the limiting block abuts against one end of the sample placement plate.

[0015] A detection process for detecting avian influenza virus antibodies, the detection process comprising the following steps: S1: Insert the sample placement plate containing the reaction chip into the rectangular socket, so that the reaction chip is in the detection chamber. Rotate the adjustment block to drive the rotating disk to rotate and move the required narrowband filter to the top of the reaction chip. S2: Rotate the adjusting screw, which will move the push rod towards the rotating disk via the moving block, insert the triangular positioning block into the positioning groove, and lock the position of the rotating disk; S3: The distance the push rod moves when it is inserted into the positioning slot is transmitted to the moving rod through the lever, which drives the moving rod to move in the moving hole. The moving rod drives the excitation light source to slide on the slide rail until the excitation light source moves to the bottom of the condenser. The excitation light source emits excitation light to excite the fluorescent marker in the sample and generate a fluorescence signal. The image sensor then converts the fluorescence signal into an electrical signal to realize antibody detection. S4: When other antibodies need to be detected, rotate the rotating disk to move other types of narrowband filters into the detection chamber, and insert one end of the push rod into the positioning slot. At this time, the moving rod will automatically move the excitation light source corresponding to the narrowband filter to the bottom of the condenser, so as to realize the detection of other antibodies.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. When multiple antibodies in avian influenza virus need to be detected, the excitation light source can be moved within the detection chamber by moving the moving rod to position the required excitation light source directly below the sample. Simultaneously, the rotating disk is rotated within the circular cavity by the adjusting block and rotating column, moving the narrowband filter corresponding to the excitation light source into the detection chamber. Different excitation light sources excite multiple fluorescent labels in the sample to generate fluorescence signals. The narrowband filter is used to filter background light and astigmatism to match the wavelength requirements of different signals, ensuring that each signal is accurately captured. Thus, by switching between multiple excitation light sources and multiple narrowband filters, multiple antibodies in avian influenza virus can be detected, improving detection efficiency.

[0017] 2. When the required narrowband filter is moved into the detection cavity, insert one end of the push rod into the positioning groove. The two inclined surfaces of the triangular positioning block abut against the two inclined surfaces of the triangular groove, ensuring that the rotating disk can be accurately locked when it is rotated to the designated position. This ensures the alignment accuracy of the narrowband filter and the excitation light source, and further improves the reliability of the detection.

[0018] 3. The depths of the three pairs of positioning slots increase sequentially. When the push rod is inserted into the positioning slots of different depths, the moving distance of the push rod is also different. At this time, through the cooperation of the support rod and the lever, the moving rod is driven to move different distances. The three different moving distances can move the three different types of excitation light sources to directly below the sample. The automatic switching of the excitation light source is realized by the positioning and locking of the rotating disk, which effectively improves the automation level of the device.

[0019] 4. The reaction chip containing the sample is placed in the placement hole of the sample placement plate. When the sample placement plate is inserted into the instrument, the piston will be inserted into the cylindrical hole and inflate the sealing bladder. After the sealing bladder is inflated, it can automatically lock the reaction chip on the sample placement plate to prevent displacement during the detection process. At the same time, the expansion of the sealing bladder can fill the gap between the reaction chip and the sample placement plate, which can prevent the excitation light from passing through the gap and affecting the recognition and detection of weak fluorescence signals.

[0020] 5. Install a condenser between the excitation source and the sample placement plate. The condenser can focus the excitation light emitted by the excitation source onto the sample, thereby increasing the intensity of the excitation light. Attached Figure Description

[0021] Figure 1This is a frontal three-dimensional structural diagram of a device for detecting avian influenza virus antibodies proposed in this invention; Figure 2 This is a side-view three-dimensional structural diagram of a device for detecting avian influenza virus antibodies proposed in this invention; Figure 3 for Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 This is a schematic diagram of a three-dimensional partial cross-sectional structure of a device for detecting avian influenza virus antibodies proposed in this invention; Figure 5 This is a three-dimensional cross-sectional structural diagram of a device for detecting avian influenza virus antibodies proposed in this invention; Figure 6 for Figure 5 Enlarged structural diagram at point B; Figure 7 A three-dimensional structural diagram of the sliding component, excitation light source, and condenser. Figure 8 A three-dimensional structural diagram of the support rod and lever; Figure 9 This is a top-view cross-sectional structural diagram of the rotating disk; Figure 10 for Figure 9 Enlarged structural diagram at point C; Figure 11 A schematic diagram of the three-dimensional cross-sectional structure of the instrument body and the sample placement plate; Figure 12 for Figure 11 Enlarged structural diagram at point D; Figure 13 This is a partial three-dimensional structural diagram of the sample placement plate.

[0022] In the diagram: 1. Instrument body, 2. Detection chamber, 3. Image sensor, 4. Sample placement plate, 5. Slide rail, 6. Sliding seat, 7. Excitation light source, 8. Moving rod, 9. Rotating disk, 10. Rotating column, 11. Adjusting block, 12. Narrow band filter, 13. Positioning groove, 1301. Rectangular groove, 1302. Triangular groove, 14. Top rod, 15. Triangular positioning block, 16. Support, 17. Adjusting screw, 18. Moving block, 19. Support rod, 20. Lever, 21. Upper strip hole, 22. Lower strip hole, 23. Placement hole, 24. Reaction chip, 25. Sealing airbag, 26. Cylindrical hole, 27. Mounting rod, 28. Piston, 29. Mounting block, 30. Spring rod, 31. Limiting block, 32. Concentrator, 33. Protective shell, 34. Rotating block. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0024] Reference Figures 1-2 A device for detecting avian influenza virus antibodies includes an instrument body 1, a detection chamber 2 inside the instrument body 1, a mounting hole at the upper end of the instrument body 1 communicating with the detection chamber 2, an image sensor 3 fixedly installed in the mounting hole, a rectangular insertion hole at the instrument body 1 communicating with the detection chamber 2, a sample placement plate 4 slidably inserted in the rectangular insertion hole, one end of the sample placement plate 4 extending into the detection chamber 2. The image sensor 3 is existing technology and can be purchased commercially. It is a semiconductor device that converts light signals into electrical signals through photoelectric conversion and finally forms a digital image. It can detect antibodies that generate fluorescent signals. It will not be described in detail here. The image sensor 3 is installed at the upper end of the detection chamber 2, and the sample placed on the sample placement plate 4 is located directly below the image sensor 3.

[0025] Reference Figures 4-5 Three different types of excitation light sources 7 are slidably installed at the bottom of the detection chamber 2 via a sliding assembly. The sliding assembly includes a slide rail 5 fixedly installed at the bottom of the detection chamber 2 and a sliding seat 6 slidably installed on the slide rail 5. The three excitation light sources 7 are equidistantly fixedly installed on the sliding seat 6. A condenser 32 is fixedly installed inside the detection chamber 2. The condenser 32 is installed between the sample placement plate 4 and the excitation light source 7. The instrument body 1 has a moving hole that communicates with the detection chamber 2. A moving rod 8 is slidably inserted into the moving hole. One end of the moving rod 8 is fixedly connected to the side of one of the excitation light sources 7.

[0026] Reference Figure 7 Three different types of excitation light sources 7 slide horizontally on the slide rail 5 via the sliding seat 6. The three different types of excitation light sources 7 can emit excitation light of different wavelengths. The three excitation light sources 7 are 405nm, 488nm and 532nm light sources, respectively. When the excitation light source 7 moves to the lower end of the condenser 32, the condenser 32 can focus the excitation light emitted by the excitation light source 7 onto the sample, thereby increasing the intensity of the excitation light. By moving the moving rod 8, the three excitation light sources 7 can be moved at the bottom of the detection cavity 2.

[0027] Reference Figures 4-5The instrument body 1 has a circular cavity inside that communicates with the detection chamber 2. The upper end of the instrument body 1 has a rotating hole that communicates with the circular cavity. A rotating disk 9 is rotatably installed inside the circular cavity. A rotating column 10 is vertically rotatably installed inside the rotating hole. The lower end of the rotating column 10 is fixedly connected to the center of the rotating disk 9. The upper end of the rotating column 10 passes through the rotating hole and is fixedly installed with an adjusting block 11. Multiple fixing holes are equidistantly opened on the circumference of the rotating disk 9. Narrow band filters 12 are fixedly installed in each of the multiple fixing holes. The multiple narrow band filters 12 can be moved into the detection chamber 2 in sequence.

[0028] The rotating disk 9 has six fixing holes, on which six narrow-band filters 12 are fixedly installed. The six narrow-band filters 12 are 450nm, 460nm, 520nm, 530nm, 570nm and 580nm filters, respectively. The 450nm and 460nm filters correspond to a 405nm light source, the 520nm and 530nm filters correspond to a 488nm light source, and the 570nm and 580nm filters correspond to a 532nm light source. When the rotating adjustment block 11 drives the rotating column 10 to rotate, it can drive the rotating disk 9 to rotate in the circular cavity. During the rotation of the rotating disk 9, different types of narrow-band filters 12 can be moved into the detection cavity 2. The three different wavelength light sources and the six different types of narrow-band filters 12 can excite a variety of fluorescent labels, thereby detecting a variety of antibodies, such as H3, H5, H7 and H9.

[0029] The rotating disk 9 has three pairs of positioning grooves 13 equidistantly spaced on its side circumference. The multiple positioning grooves 13 correspond to multiple fixing holes. The positioning grooves 13 are composed of rectangular grooves 1301 and triangular grooves 1302. The instrument body 1 has a sliding hole on its side that communicates with the circular cavity. A push rod 14 is horizontally slidably installed in the sliding hole. A triangular positioning block 15 is fixedly installed at one end of the push rod 14 near the rotating disk 9. The triangular positioning block 15 is inserted into one of the triangular grooves 1302.

[0030] Reference Figure 6 A bracket 16 is fixedly installed on the side of the instrument body 1. A threaded hole is opened on the bracket 16, and an adjusting screw 17 is installed in the threaded hole. One end of the adjusting screw 17 is rotatably installed on the instrument body 1, and a rotating block 34 is fixedly installed on the other end. A moving block 18 is threadedly connected to the adjusting screw 17. The lower end of the moving block 18 is fixedly connected to the top rod 14. When the top rod 14 moves, it can drive the adjusting screw 17 to rotate by rotating the rotating block 34, thereby driving the moving block 18 to move on the adjusting screw 17. The movement of the moving block 18 drives the top rod 14 to move.

[0031] Reference Figure 9 and Figure 10The depth of the three pairs of positioning grooves 13 increases sequentially along the rotation direction of the rotating disk 9. There are six positioning grooves 13 in total, and the positioning grooves 13 have three depths. The shallowest positioning groove 13 corresponds to 450nm and 460nm filters, the deepest positioning groove 13 corresponds to 570nm and 580nm filters, and the middle depth positioning groove 13 corresponds to 520nm and 530nm filters. The inclination of the two inclined surfaces of the triangular positioning block 15 is the same as the inclination of the two inclined surfaces of the triangular groove 1302. When the triangular positioning block 15 is fully inserted into the triangular groove 1302, the two inclined surfaces of the triangular positioning block 15 just abut against the two inclined surfaces of the triangular groove 1302, which can accurately position and lock the rotating disk 9.

[0032] Reference Figure 6 and Figure 8 The instrument body 1 has a transmission component on its side for moving the moving rod 8. A support rod 19 is fixedly installed on the side of the instrument body 1. A lever 20 is rotatably installed at one end of the support rod 19. The rotatable connection between the support rod 19 and the lever 20 is located in the upper part of the lever 20. An upper slot 21 is opened at the upper end of the lever 20, and a lower slot 22 is opened at the lower end of the lever 20. The end of the top rod 14 away from the triangular positioning block 15 is slidably hinged in the upper slot 21. One end of the moving rod 8 is slidably hinged in the lower slot 22 of the lever 20. A protective shell 33 is installed on the side of the instrument body 1. The adjusting screw 17, the moving block 18 and the lever 20 are all inside the protective shell 33.

[0033] When the push rod 14 moves, one end of it slides in the upper slot 21 of the lever 20, while driving the lever 20 to rotate at one end of the support rod 19. The movement of the push rod 14 can drive the moving rod 8 to move in the moving hole through the lever 20. When the push rod 14 is inserted into the positioning groove 13 at different depths, the distance the push rod 14 moves will be different, and the distance the moving rod 8 moves through the lever 20 will also be different. When the push rod 14 moves in the positioning groove 13 at three different depths, the moving rod 8 and the lever 20 can move the three different types of excitation light sources 7 to the lower end of the focusing cover 32 respectively.

[0034] Reference Figures 11-13The sample placement plate 4 has a stepped placement hole 23 located inside the detection chamber 2. A reaction chip 24 is placed inside the placement hole 23. A U-shaped mounting groove is provided on the side of the placement hole 23. A sealing airbag 25 is fixedly installed in the mounting groove. A cylindrical hole 26 is provided at one end of the sample placement plate 4 near the detection chamber 2. One end of the cylindrical hole 26 is connected to the inside of the sealing airbag 25. An inflation component is provided inside the detection chamber 2 for inflating the sealing airbag 25. A locking component is installed on the front side of the instrument body 1 for locking the sample placement plate 4. The stepped placement hole 23 can be used to place the reaction chip 24 containing antibodies. The reaction chip 24 is a microfluidic immunofluorescence chip. The chip integrates multiple independent reaction chambers for antigen-specific immune binding reactions. After the immune reaction is completed, a fluorescent labeling process is performed in the reaction chamber. When the reaction chip 24 is placed in the placement hole 23, the sealing airbag 25 surrounds the reaction chip 24.

[0035] The inflation assembly includes a mounting rod 27 horizontally fixedly installed in the detection chamber 2 and a piston 28 fixedly installed at one end of the mounting rod 27. The piston 28 and the cylindrical hole 26 are located on the same horizontal line. The diameter of the piston 28 is the same as the diameter of the cylindrical hole 26. When the sample placement plate 4 is inserted into the instrument, the piston 28 will be inserted into the cylindrical hole 26. The side of the piston 28 is sealed with the inner wall of the cylindrical hole 26. When the piston 28 moves in the cylindrical hole 26, it can inject the air in the cylindrical hole 26 into the sealing airbag 25, causing the sealing airbag 25 to inflate.

[0036] Reference Figure 3 The locking assembly includes a mounting block 29 fixedly mounted on the instrument body 1, two spring rods 30 symmetrically fixedly mounted on the mounting block 29, and a limiting block 31 with a right-angled trapezoidal longitudinal section fixedly mounted on one end of the two spring rods 30. When the sample placement plate 4 is inserted into the rectangular insertion hole, the inclined surface of the limiting block 31 abuts against one end of the sample placement plate 4. At this time, the spring rods 30 are in a compressed state. Under the action of the elastic force of the spring rods 30, the limiting block 31 locks the sample placement plate 4 in the rectangular insertion hole. When it is necessary to remove the sample placement plate 4, the limiting block 31 is moved upward, and the spring rods 30 are further compressed, so that the limiting block 31 is separated from the sample placement plate 4.

[0037] The detection process for avian influenza virus antibodies includes the following steps: S1: Insert the sample placement plate 4 containing the reaction chip 24 into the rectangular socket so that the reaction chip 24 is in the detection cavity 2. Rotate the adjustment block 11 to drive the rotating disk 9 to rotate and move the narrow band filter 12 to be used directly above the reaction chip 24. S2: Rotate the adjusting screw 17, and drive the top rod 14 to move towards the rotating disk 9 through the moving block 18, insert the triangular positioning block 15 into the positioning groove 13, and lock the position of the rotating disk 9. S3: The distance that the top rod 14 moves after being inserted into the positioning slot 13 is transmitted to the moving rod 8 through the lever 20, which drives the moving rod 8 to move in the moving hole. The moving rod 8 drives the excitation light source 7 to slide on the slide rail 5 until the excitation light source 7 moves to the lower end of the condenser 32. The excitation light source 7 emits excitation light to excite the fluorescent markers in the sample and generate a fluorescence signal. The image sensor 3 then converts the fluorescence signal into an electrical signal to realize antibody detection. S4: When other antibodies need to be detected, rotate the rotating disk 9 to move other types of narrow band filters 12 into the detection chamber 2, and insert one end of the top rod 14 into the positioning groove 13. At this time, the moving rod 8 will automatically move the excitation light source 7 corresponding to the narrow band filter 12 to the lower end of the condenser 32 to realize the detection of other antibodies.

[0038] In this invention, firstly, various fluorescent agents are added to the reaction chip 24 to specifically bind with avian influenza virus antibodies, completing the fluorescent labeling process. Then, the reaction chip 24 containing avian influenza virus antibodies is placed in the placement hole 23 of the sample placement plate 4, with the sealing airbag 25 in contact with the reaction chip 24. Next, the sample placement plate 4 is inserted into the rectangular insertion hole, and the reaction chip 24 is sent into the detection chamber 2. During this process, the piston 28 installed at one end of the mounting rod 27 is inserted into the cylindrical hole 26, and the piston 28 squeezes the air in the cylindrical hole 26 into the sealing airbag 25, causing the sealing airbag 25 to expand. After the sealing airbag 25 is inflated, it can automatically lock the reaction chip 24 on the sample placement plate 4, preventing it from shifting during the detection process. At the same time, the expansion of the sealing airbag 25 can fill the gap between the reaction chip 24 and the sample placement plate 4, preventing the excitation light emitted by the excitation light source 7 from passing through the gap and directly irradiating the narrow band filter 12, ensuring the recognition and detection of weak fluorescence signals.

[0039] During testing, rotating the adjusting block 11 drives the rotating column 10 to rotate, which in turn drives the rotating disk 9 to rotate within the circular cavity, moving the required narrowband filter 12 into the testing cavity 2. Then, rotating the rotating block 34 drives the adjusting screw 17 to rotate. The rotation of the adjusting screw 17 drives the moving block 18 to move towards the instrument body 1. The moving block 18 drives the top rod 14 to move towards the rotating disk 9. At the same time, the top rod 14 drives the triangular positioning block 15 to insert into the positioning groove 13 on the side of the rotating disk 9 until the two inclined surfaces of the triangular positioning block 15 abut against the two inclined surfaces of the triangular groove 1302. This accurately locks the rotating disk 9 in the designated position, ensuring that the narrowband filter 12 is aligned with the sample.

[0040] When the movable block 18 moves the push rod 14, one end of the push rod 14 slides in the upper slot 21 of the lever 20, thereby causing the lever 20 to rotate at one end of the support rod 19. When the lever 20 rotates, it transmits the horizontal movement of the push rod 14 to the movable rod 8, thereby causing the movable rod 8 to move horizontally within the movable hole. The hinge between the movable rod 8 and the lever 20 slides in the lower slot 22. When the movable rod 8 moves, it causes the sliding seat 6 to slide on the slide rail 5, thereby simultaneously moving the three excitation light sources 7. When the triangular positioning block 1... When the 5 is fully inserted into the triangular groove 1302, one of the excitation light sources 7 moves to the lower end of the condenser 32. The excitation light source 7 emits excitation light to excite the corresponding fluorescent marker in the sample and generate a fluorescence signal. The image sensor 3 then converts the fluorescence signal into an electrical signal to realize antibody detection. Different excitation light sources 7 emit excitation light of different wavelengths to excite the corresponding fluorescent markers in various fluorescent markers and generate fluorescence signals. Thus, when switching between different excitation light sources 7 and different narrowband filters 12, multiple fluorescence signals can be displayed in turn.

[0041] When other antibodies need to be detected, the push rod 14 and the triangular positioning block 15 are moved out of the positioning groove 13, and the adjusting block 11 is rotated again to drive the rotating disk 9 to rotate, so that other types of narrow band filters 12 are moved into the detection chamber 2. Then the push rod 14 and the triangular positioning block 15 are inserted into the positioning groove 13 to lock the rotating disk 9.

[0042] The depth of the multiple pairs of positioning grooves 13 on the side of the rotating disk 9 increases sequentially. When the top rod 14 and the triangular positioning block 15 are inserted into the shallowest positioning groove 13, the top rod 14 moves the shortest distance. At this time, the excitation light source 7 on the sliding seat 6 that is closest to the moving rod 8 moves to the bottom of the condenser hood 32. When the top rod 14 and the triangular positioning block 15 are inserted into the deepest positioning groove 13, the excitation light source 7 on the sliding seat 6 that is farthest from the moving rod 8 moves to the bottom of the condenser hood 32. When the top rod 14 and the triangular positioning block 15 are inserted into the positioning groove 13 of the middle depth, the middle excitation light source 7 on the sliding seat 6 moves to the bottom of the condenser hood 32. While the rotating disk 9 is locking the positioning, it can realize the automatic switching of the excitation light source 7, without the need for the testing personnel to manually adjust the excitation light source 7, effectively improving the automation level of the device.

[0043] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A device for detecting avian influenza virus antibodies, comprising an instrument body (1), characterized in that, The instrument body (1) has a detection cavity (2) inside. The upper end of the instrument body (1) has a mounting hole that communicates with the detection cavity (2). An image sensor (3) is fixedly installed in the mounting hole. The instrument body (1) has a rectangular insertion hole that communicates with the detection cavity (2). A sample placement plate (4) is slidably inserted in the rectangular insertion hole. One end of the sample placement plate (4) extends into the detection cavity (2). The bottom of the detection chamber (2) is slidably mounted with three different types of excitation light sources (7) via a sliding assembly. The instrument body (1) has a movable hole that communicates with the detection chamber (2). A movable rod (8) is slidably inserted into the movable hole. One end of the movable rod (8) is fixedly connected to the side of one of the excitation light sources (7). The instrument body (1) has a circular cavity that communicates with the detection cavity (2) inside. The upper end of the instrument body (1) has a rotating hole that communicates with the circular cavity. A rotating disk (9) is rotatably installed inside the circular cavity. A rotating column (10) is vertically rotatably installed inside the rotating hole. The lower end of the rotating column (10) is fixedly connected to the center of the rotating disk (9). The upper end of the rotating column (10) passes through the rotating hole and is fixedly installed with an adjusting block (11). Multiple fixing holes are equidistantly opened on the circumference of the rotating disk (9). Narrow band filters (12) are fixedly installed in each of the multiple fixing holes. The multiple narrow band filters (12) can be moved into the detection cavity (2) in sequence. The sliding assembly includes a slide rail (5) fixedly installed at the bottom of the detection chamber (2) and a sliding seat (6) slidably installed on the slide rail (5). The three excitation light sources (7) are fixedly installed at equal intervals on the sliding seat (6). The number of fixing holes is six. The rotating disk (9) has three pairs of positioning grooves (13) equidistantly arranged on the side circumference. The multiple positioning grooves (13) correspond to multiple fixing holes. The positioning grooves (13) are composed of rectangular grooves (1301) and triangular grooves (1302). The instrument body (1) has a sliding hole connected to the circular cavity on the side. A top rod (14) is horizontally slidably installed in the sliding hole. A triangular positioning block (15) is fixedly installed at one end of the top rod (14) near the rotating disk (9). The triangular positioning block (15) is inserted into one of the triangular grooves (1302). The depth of the three pairs of positioning grooves (13) increases sequentially along the rotation direction of the rotating disk (9). A support rod (19) is fixedly installed on the side of the instrument body (1). A lever (20) is rotatably installed at one end of the support rod (19). The rotatable connection between the support rod (19) and the lever (20) is located in the upper half of the lever (20). An upper strip hole (21) is opened at the upper end of the lever (20), and a lower strip hole (22) is opened at the lower end of the lever (20). The end of the top rod (14) away from the triangular positioning block (15) is slidably hinged in the upper strip hole (21), and one end of the moving rod (8) is slidably hinged in the lower strip hole (22) opened in the lever (20).

2. The device for detecting avian influenza virus antibodies according to claim 1, characterized in that, A focusing cover (32) is fixedly installed inside the detection cavity (2), and the focusing cover (32) is installed between the sample placement plate (4) and the excitation light source (7).

3. The apparatus for detecting avian influenza virus antibodies according to claim 2, characterized in that, A bracket (16) is fixedly installed on the side of the instrument body (1). A threaded hole is provided on the bracket (16). An adjusting screw (17) is threaded in the threaded hole. One end of the adjusting screw (17) is rotatably installed on the instrument body (1), and a rotating block (34) is fixedly installed on the other end. A moving block (18) is threaded on the adjusting screw (17). The lower end of the moving block (18) is fixedly connected to the top rod (14). A protective shell (33) is installed on the side of the instrument body (1). The adjusting screw (17), the moving block (18), and the lever (20) are all inside the protective shell (33).

4. The device for detecting avian influenza virus antibodies according to claim 3, characterized in that, The sample placement plate (4) has a stepped placement hole (23) located inside the detection chamber (2). A reaction chip (24) is placed inside the placement hole (23). A U-shaped mounting groove is provided on the side of the placement hole (23). A sealing airbag (25) is fixedly installed in the mounting groove. A cylindrical hole (26) is provided at one end of the sample placement plate (4) near the detection chamber (2). One end of the cylindrical hole (26) is connected to the inside of the sealing airbag (25). An inflation component for inflating the sealing airbag (25) is provided inside the detection chamber (2). A locking component for locking the sample placement plate (4) is installed on the front side of the instrument body (1).

5. The apparatus for detecting avian influenza virus antibodies according to claim 4, characterized in that, The inflation assembly includes a mounting rod (27) horizontally fixedly installed in the detection chamber (2) and a piston (28) fixedly installed at one end of the mounting rod (27). The piston (28) and the cylindrical hole (26) are located on the same horizontal line, and the diameter of the piston (28) is the same as the diameter of the cylindrical hole (26).

6. The apparatus for detecting avian influenza virus antibodies according to claim 5, characterized in that, The locking assembly includes a mounting block (29) fixedly mounted on the instrument body (1), two spring rods (30) symmetrically fixedly mounted on the mounting block (29), and a limiting block (31) with a right-angled trapezoidal longitudinal section fixedly mounted on one end of the two spring rods (30). The inclined surface of the limiting block (31) abuts against one end of the sample placement plate (4).

7. A detection method for an apparatus for detecting avian influenza virus antibodies according to any one of claims 4-6, characterized in that, The detection method includes the following steps: S1: Insert the sample placement plate (4) containing the reaction chip (24) into the rectangular socket so that the reaction chip (24) is in the detection cavity (2). Rotate the adjustment block (11) to drive the rotating disk (9) to rotate and move the required narrow band filter (12) directly above the reaction chip (24). S2: Rotate the adjusting screw (17), and drive the top rod (14) to move towards the rotating disk (9) through the moving block (18), insert the triangular positioning block (15) into the positioning groove (13), and lock the position of the rotating disk (9); S3: The distance that the top rod (14) moves when inserted into the positioning slot (13) will be transmitted to the moving rod (8) through the lever (20), which will drive the moving rod (8) to move in the moving hole. The moving rod (8) will drive the excitation light source (7) to slide on the slide rail (5) until the excitation light source (7) moves to the lower end of the condenser (32). The excitation light source (7) will emit excitation light to excite the fluorescent markers in the sample and generate a fluorescence signal. The image sensor (3) will then convert the fluorescence signal into an electrical signal to realize antibody detection. S4: When other antibodies need to be detected, rotate the rotating disk (9) to move other narrowband filters (12) into the detection chamber (2), and insert one end of the top rod (14) into the positioning groove (13). At this time, the moving rod (8) will automatically move the excitation light source (7) corresponding to the narrowband filter (12) to the lower end of the condenser (32) to realize the detection of other antibodies.

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